The substitutes begin operating as members of the Shinsengumi. Their first job is escorting Sakuma Shozan. However, something seems to have happened between Sakuya and Shozan in the past...
I am Shinsengumi Vice-Commander 
Hijikata Toshizo.
Ich bin der Vize-Kommandant
Hijikata Toshizō.
Soy el segundo al mando
del Shinsengumi, Hijikata Toshizou.
Soy el segundo al mando
del Shinsengumi, Hijikata Toshizou.
Je suis Toshizô Hijikata,
vice-capitaine du Shinsengumi.
Sono il vice-comandante
della Shinsengumi, Hijigata Toshizo.
Sou o Vice-Comandante
do Shinsengumi, Toshizou Hijikata.
Я заместитель командующего
Синсэнгуми, Тосидзё Хидзиката.