When Mana visits GeGeGe Forest, she finds Kitaro and Ratman arguing over something trivial. They each declare for the I-don't-know-how-many-th time that they're cutting ties with each other. Meanwhile, in town, the water has been contaminated sending everyone running around looking for drinking water. No one knows why the water is polluted, and no attempts at purifying it work. Kitaro believes that the one behind the pollution incident is one of the Four Generals of Betrayal. Kitaro begins an investigation, but doesn't know how to stop the pollution of the water. Meanwhile, a survivor of the Way of the Demon Society, Isurugi Rei, begins to move again...!?
During the Ansei period
under the Tokugawa Shogunate,
،خلال عهد أنسي، تحت عهدة الشوغن توكوغاوا
In der Ansei-Periode der Edo-Zeit
Durante la era Edo,
contaminó el agua de pozos y ríos
Durante la era Edo,
contaminó el agua de pozos y ríos
Durant la période Ansei,
il a pollué puits et rivières d’Edo.
Si dice che nell'era Ansei abbia contaminato
 l'acqua dei pozzi e dei fiumi di tutta Edo
Durante a era Ansei,
sob o Xogunato Tokugawa,
Во время эры Ансэй, 
когда правил сёгунат Токугава,