The substitutes begin operating as members of the Shinsengumi. Their first job is escorting Sakuma Shozan. However, something seems to have happened between Sakuya and Shozan in the past...
After all, you'll be dead before 
this sand finishes falling.
Denn noch vor dem letzten Sandkorn
wirst du ein toter Mann sein.
Morirás antes
de que se acabe esta arena.
Morirás antes
de que se acabe esta arena.
Après tout, quand le sable sera écoulé,
tu seras mort.
Dopotutto, sarai morto prima che
la sabbia finisca di scendere.
Afinal, você estará morto antes
desta areia terminar de cair.
Ведь всё равно после падения
песка ты погибнешь.