The substitutes begin operating as members of the Shinsengumi. Their first job is escorting Sakuma Shozan. However, something seems to have happened between Sakuya and Shozan in the past...
I won't allow you to kill Sakuma Shozan.
Ich werde nicht zulassen,
dass du Sakuma Shōzan tötest.
No dejaré que maten a Sakuma Shouzan.
No dejaré que matéis a Sakuma Shouzan.
Je ne tuerai pas Shôzan Sakuma.
Non ti permetterò di uccidere Sakuma Shozan.
Eu não vou deixar que você
mate o Shozan Sakuma.
Я не дам вам убить Сёдзана Сакуму.