Steroidal glycosides from the bulbs of Fritillaria meleagris and their cytotoxic activities.
Steroidal glycosides (1-18), including 10 new compounds (1-10), were isolated from the bulbs of Fritillaria meleagris (Liliaceae). The structures of the new compounds were determined by two-dimensional (2D) NMR analysis, and by hydrolytic cleavage followed by spectroscopic and chromatographic analysis. The isolated compounds and their aglycones were evaluated for cytotoxic activity against HL-60 human promyelocytic leukemia cells and A549 human lung adenocarcinoma cells. Morphological observation and flow cytometry analysis showed that 5β-spirostanol glycoside (2) and a cholestane derivative (17a) induced apoptotic cell death in HL-60 cells through different mechanisms of action. Furthermore, the (22R)-spirosolanol glycoside (11) selectively induced apoptosis in A549 cells without affecting the caspase-3 activity level.
T1 T2 T3 T4 T5 T6
CHEMICAL CHEMICAL CHEMICAL CHEMICAL CHEMICAL GENE-Y
Steroidal glycosides 5β-spirostanol glycoside cholestane (22R)-spirosolanol glycoside Steroidal glycosides caspase-3