High salt diets during pregnancy affected fetal and offspring renal renin-angiotensin system.
Intrauterine environments are linked to fetal renal development and postnatal health. Influence of salt diets during pregnancy on renal functions and renin-angiotensin system (RAS) were determined in the ovine fetus and offspring. Pregnant ewes were fed high-salt diet (HSD) or normal-salt diet (NSD) for two months during middle-later gestation. Fetal renal functions, plasma hormones, mRNA and protein expressions of the key elements of renal RAS were measured in the fetus and offspring. Fetal renal excretion of sodium was increased while urine volume decreased in HSD group. Fetal blood urea nitrogen was increased, while kidney/body weight ratio decreased in HSD group. The altered ratio also was observed in the offspring at 15-day-old and 90-day-old. Maternal and fetal plasma antidiuretic hormone was elevated without change in plasma renin activity and Ang I levels, while plasma Ang II was decreased. The key elements of local renal RAS, including angiotensinogen, ACE, ACE2, AT1 and AT2 receptor expression in both mRNA and protein, except renin, were altered following maternal high salt intake. The results suggest that High intake of salt during pregnancy affected fetal renal development associated with an altered expression of the renal key elements of RAS, some alterations of fetal origins remained after birth as possible risks in developing renal or cardiovascular diseases.
T10 T11 T12 T13 T14 T15 T16 T17 T1 T2 T3 T4 T5 T6 T7 T8 T9
GENE-Y GENE-Y GENE-Y GENE-Y GENE-Y GENE-Y GENE-Y GENE-Y CHEMICAL CHEMICAL CHEMICAL GENE-Y GENE-Y GENE-Y GENE-Y GENE-Y GENE-Y
Ang II angiotensinogen ACE ACE2 AT1 AT2 receptor renin angiotensin sodium urea nitrogen renin renin angiotensin antidiuretic hormone renin Ang I