Ample experimental evidence indicates that intracerebral injection or infusion of amyloid-beta peptides (Aβ) to rodents induces learning and memory impairments as well as neurodegeneration in brain areas related to cognitive function. In the present study, we assessed the effects of a single intracerebroventricular (i.c.v.) injection of aggregated Aβ fragment (25–35) at a dose of 15nmol/rat on short-term and long-term memory in rats during the 6-month post-surgery period. The results demonstrate that Aβ(25–35)-induced memory impairments in spontaneous alternation behavior in a Y-maze at 17, 36, and 180 days after the surgery as well as in a social recognition task 110 days post-surgery. Aβ(25–35) also impaired spatial memory in an 8-arm radial maze, but did not influence performance of the step-down passive avoidance task. These results suggest that Aβ(25–35) preferably induces impairments of spatial and non-spatial short-term (working) memory rather than long-term memory in rats.