Quasi-equilibrium potential profiles of stoichiometric LiMn2O4 spinel in charge–discharge process have been recorded to investigate temperature dependence in lithium ions intercalation. Temperature of electrodes has been controlled within 273≤T≤298K in this research. Discharge curve reveals anomalous stepwise features when both charge and discharge processes are carried out at low temperature (T≤278K). However, discharge curve at 273K immediately after charge at 298K does not show stepwise shape but gently slope as potential curve in charge at 298K. In contrast to this, discharge curve at 298K immediately after charge at 273K shows stepwise anomaly. Temperature at discharge does not affect on shape of potential profile at next charge process. This temperature hysteresis has been confirmed repeatedly in several charge–discharge cycles. Temperature at Li ions de-intercalation has a large effect on next intercalation in LiMn2O4 spinel.