The decreased participation in physical activity by children with probable developmental coordination disorder (pDCD) has raised concerns about their aerobic fitness and lung function levels. The purpose of the present study was to examine assessment of cardiorespiratory and neuromotor fitness, using laboratory-based tests during an incremental treadmill protocol in healthy children with and without pDCD. Twenty sex children ages 6–9 years took part in this study. Motor coordination was assessed using the Movement Assessment Battery for Children (MABC). All participants performed a cardiopulmonary exercise test (CPET) on a cycle ergometer. Pulmonary function was assessed by spirometric measurements (forced vital capacity: FVC, forced expiratory volume in 1s: FEV1) and walking distance (6MWD) was assessed using the 6-min walking test. The children with pDCD had lower VO2max than children without pDCD (p <0.01). Moreover, FVC and FEV1 were significantly higher in children without pDCD than in children with the disorder (p <0.05, p <0.01 respectively). Likewise, children with pDCD had poorer performance on the 6MWD than children without pDCD (p <0.01). A significant correlation between the absolute value for FEV1 and 6MWD (r =0.637, p <0.05) in pDCD group was observed. We found a significant correlation between VO2max and MABC score (r =−0.612, p <.001) and between VO2max and 6MWD (r =0.502, p <.001) for all children. Moreover, a significant correlation between VO2max and FEV1 (r =0.668, p <.05) was found in children with pDCD. Overall, the reduced aerobic capacity of DCD was associated with decreased of lung function, as well as an alteration of peripheral muscle responses.