Li4Ti5O12 with long cycling life has been deemed to be one of the most promising anode materials for lithium ion battery. Nevertheless, poor electronic conductivity impedes its application for energy storage. Meanwhile MXene possesses high electrical conductivity and moderate electrochemical energy storage. Herein, we develop a novel strategy for the in-situ synthesis of layered-stacked Li4Ti5O12-MXene composite. The synergistic effect of Li4Ti5O12 and MXene greatly improved the electrochemical properties of Li4Ti5O12-MXene composite. At a high current density of 10Ag−1, a high discharge capacity of 116mAhg−1 can be achieved. Moreover, a high discharge capacity of 178mAhg−1 after 500cycles can be maintained at a current density of 5Ag−1. This work demonstrates a scalable route to assemble MXene-derived Ti-based materials for high-performance electrochemical energy storage applications.