A quick and efficient approach to prepare nitrogen doped graphene (NG) is proposed in this paper via microwave heating in NH3 atmosphere. Results show that graphene, as an allotrope of carbon, is a good microwave-absorbing material and can reach a high temperature in minutes, facilitating nitrogen incorporation into the structure under NH3. Elemental analysis and X-ray photoelectron spectroscope (XPS) verified the success of N-doping with the nitrogen content of 5.04wt%. For comparison, both plain grapheme (G) and the NG were used as supporting materials for platinum to investigate their potential application in fuel cells. Transmission electron microscope (TEM) images showed that the NG improved the distribution of Pt particles. Themogravimetry (TG) and differential scanning calorimeters (DSC) revealed better thermal stability of the Pt/NG than that of the Pt/G. Furthermore, the Pt/NG catalysts exhibited higher electrochemical active surface area, methanol catalytic activity, and tolerance to CO poisoning than those of the Pt/G under fuel cell conditions. It suggests that the NG prepared by microwave synthesis has provided a new way to improve electrocatalytic activity in fuel cells.