Research into the development of direct methanol alkaline fuel cell (DMAFC) using an anion exchange polymer electrolyte membrane is described. The commercial membrane used had a higher electric resistance, but a lower methanol diffusion coefficient than Nafion® membranes. Fuel cell tests were performed using carbon supported Pt catalyst, and the effect of temperature, methanol concentration, methanol flow rate, air pressure and Pt loading were investigated. It was found that the cell performance improved drastically with a membrane assembly electrode (MEA) which did not include the gas diffusion layer on the anode, because of lower reactant mass transfer resistance. To give suitable cathode performance, humidification of the air and a subtle balance between the air pressure and water transport is required.