The formation of metallic lithium on the negative graphite electrode in a lithium-ion (Li-ion) battery, also known as lithium plating, leads to severe performance degradation and may also affect the cell safety. This study is focused on the nondestructive characterization of the aging behavior during long-term cycling at plating conditions, i.e. low temperature and high charge rate. A commercial graphite/LiFePO4 Li-ion battery is investigated in order to elucidate the aging effects of lithium plating for real-world purposes. It is shown that lithium plating can be observed as a loss of cyclable lithium which affects the capacity balance of the electrodes. In this way, lithium plating counteracts its own occurrence during prolonged cycling. The capacity losses due to lithium plating are therefore decreasing at higher cycle numbers and the capacity retention curve exhibits an inflection point. It is further shown that the observed capacity fade is partly reversible. Electrochemical impedance spectroscopy (EIS) reveals a significant increase of the ohmic cell resistance due to electrolyte consumption during surface film formation on the plated lithium. Additional cell opening provides important quantitative information regarding the thickness of the lithium layer and the corresponding mass of the plated lithium.