A three-dimensionally (3D) patterned Li4Ti5O12 electrode was fabricated by using a micro printing technology. Composite slurry including Li4Ti5O12, acetylene black, poly(vinylidene difluoride) and N-methyl pyrolidone was applied onto a current collector. The electrode has many lined patterns with high aspect ratio that stand in line on the current collector. The cyclic voltammograms of the half cell with 3D patterned electrode shows that the prepared electrode worked effectively as anode of a rechargeable lithium-ion battery. The cell with the 3D patterned electrode showed much better charge and discharge capacities than a conventional cell with a flat Li4Ti5O12 electrode at high rates. It was also confirmed that the cycle performance of the cell with 3D patterned electrode was equal or better than that with the conventional electrode.