The poly(o-anisidine)–sulfuric acid–glucose oxidase (POA–H2SO4–GOx) electrode has been investigated in the present work. Platinum electrode was used for the synthesis of poly (o-anisidine)–sulfuric acid (POA–H2SO4) film using galvanostatic method with 0.2 M o-anisidine, 1.0 M H2SO4 solution, 1.0 pH and 2 mA/cm2 applied current density. The synthesized film was characterized using electrochemical technique, conductivity measurement, UV–visible spectroscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy. GOX was immobilized on synthesized POA–H2SO4 film by cross-linking via glutaraldehyde in phosphate and acetate buffer. The Michaelis–Menten constant (
Since the first demonstration by Clark and Lyons that an enzyme could be integrated into an electrode to form a biosensor, the developments of such electrochemical devices have made considerable progress [1–3]. The advantages associated with these devices are their high selectivity and simple use in complex media as well as the possibility of developing compact and portable analyzers. Several conducting polymers such as polypyrole, poly(N-methylpyrole), polyaniline, poly(o-anisidine), poly(o-toluidine), etc. have been used to immobilize desired enzymes (glucose oxidase, urease, cholesterol oxidase, etc.) using physical adsorption and entrapment techniques [4–9]. Polyaniline and its derivatives are considered to be an attractive class of polymer because this conducting material exhibits two redox couples in the right potential range to facilitate an enzyme–polymer charge transfer. Besides, polyaniline has shown variety of applications such as in rechargeable batteries, electrocatalysis, electrochromic displays, gas separation, and biosensors. However, the role of structural and mechanical behavior of polyaniline and its derivatives for their applications to biosensor is yet to be explored.
