Cation (Mg and Al)-substituted spinel were synthesized using metal oxide precursor by co-precipitation method. XRD revealed that the prepared substituted spinel has spinel structure with Fd3m space group. In order to compensate the decreased initial capacity of cation-substituted spinel, partial anion (F) substitution was also carried out. The cycling performance of all the substituted spinel was improved, compared to the Li1.05Mn1.95O4 at 55°C. Li1.05Al0.1Mn1.85O3.95F0.05 showed better capacity retention than the other substituted spinels. Both cation and anion substitution appeared to be effective for improving the cycling performance of spinel material at elevated temperature.