Distress tolerance represents the capacity to bear and manage negative psychological states associated with distress. Low distress tolerance is a transdiagnostic construct implicated in many psychopathological conditions. The Distress Tolerance Scale (DTS) is a 15-item self-report instrument specifically designed to assess this construct. The aim of this study was to investigate the psychometric properties of the Italian version of the DTS. Factor structure, internal consistency, temporal stability, and construct validity were investigated in 477 participants from the general population. Exploratory and confirmatory factor analyses supported the one-factor structure of the DTS. The scale also showed good internal consistency, temporal stability, and construct validity. These findings suggest that the Italian version of the DTS retains the adequate psychometric properties of the original and suggest that it can be confidently used as an assessment tool of distress tolerance in both clinical and research settings.