Herein, a layer in layer nanocomposition of flower-shaped MoS2 nanoclusters with nitrogen-doped reduced graphene oxide(N-RGO@MoS2) has been successfully prepared by a facile hydrothermal method to build up 3D matrix for supporting sulfur. This approach will integrate the advantages and avoid the disadvantages of graphene and MoS2 through synergetic effect to produce an outstanding Li-S battery cathode, such as high electronic conductivity of N-RGO to reach great rate performance; stronger interaction between MoS2 and polysulfide to suppress the shuttle effect for an improved durability; the great compatibility between two 2D components avoids their self-aggregation to increase capacity through wrap around and adhesion effect with loaded sulfur. A series of samples with various content of MoS2 are investigated to optimize the electrode performance. Finally, N-RGO@MoS2-10/S delivers an optimal discharge capacity of 729.1mAhg−1 at 0.3C with the coulombic efficiency of 97.3% after 180 cycles.