This study presents partial results of an extensive research project called Health-Enhancing and Health-Threatening Behaviour: Determinants, Models, and Consequences (GA13-19808S) and explores selected factors of health-promoting behaviour of Czech adolescents. The study discussed the interconnections found between components of health-related behaviour of adolescents during a key stage of their development. In addition, we also aimed to study possible developmental trend of the above-mentioned correlates during adolescence. The subjects participating in the above-described broad research project were administered an extensive battery of tests, and as a part of the presented study, the descriptive data concerning the subjects’ age and sex, the Health-Related Behaviour Scale (Dosedlová et al., 2013), the Subjective Health Problems Inventory (modified version of the inventory by Osecká et al., 1998), Mental Health Continuum, MHC-SF (Keyes, 2005) and the Big Five Inventory (O.P. Donahue, R.I. Kentle, 1991) were all used. The research sample consisted of 735 adolescents (47.4% women and 52.6% men) aged 12-19 years (35.3% in the period of early and middle adolescence and 64.7% in the period of late adolescence). In cluster analysis, six different life styles were defined using five health-related factors. Analyses of variance were conducted to examine whether adolescents with different life-styles differ in chosen personal characteristics.