A miniature microbial fuel cell (mini-MFC) is described that utilizes an aerobic culture of Shewanella oneidensis DSP10 as the active electrochemical species in the anode chamber. We find that the maximum aerobic mini-MFC power without the addition of exogenous mediators was 0.40mW, a 33% decrease when compared with an anaerobic DSP10 culture (0.6mW) operating in the mini-MFC. This decrease is most likely due to the presence of dissolved oxygen in the anode chamber that scavenges electrons to form water, thereby reducing the number of electrons donated to the anode. Aerobic power and current density at maximum power using the true surface area of the anode (611cm2) were calculated to be 6.5mWm−2 and 13mAm−2. The power density rises to 2.0Wm−2 and 330Wm−3 when calculated using the cross-sectional area and volume of the device (2cm2, 1.2cm3). The Coulombic efficiency was also reduced from 11 to 5% when using the aerobic versus anaerobic culture. Similar results were found when the external mediator anthraquinone-2,6-disulfonate (AQDS) was added to the aerobic culture, resulting in a maximum power of 0.54mW, a 37% drop in power when compared to the anaerobic mediated system.