The effects of temperature on the dissolution of solid electrolyte interface (SEI) films in the propylene carbonate (PC)-based electrolyte are investigated. The SEI films on the mesocarbon microbeads (MCMB) electrode of the Li|1.0M LiPF6-PC/DEC (= 7/3, v/v)|MCMB cell are characterized by cyclic voltammetry, scanning electron microscopy (SEM), AC impedance, X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy. The SEM and electrochemical results show that the MCMB electrode cannot undergo the lithiation and delithiation process at 30°C due to the co-intercalation of PC and the exfoliation of MCMB. By contrast, the Li∥MCMB cell can be successfully discharged and charged at 0°C because a PC-based SEI film forms on MCMB. The energy capacity of the MCMB electrode is 281.3 mAh g−1 at 0.2C. However, the SEI film dissolves in the PC-based electrolyte as the temperature is increased to 30°C. The results of Raman spectroscopy and XPS also confirm that the composition of the PC-based SEI formed at 0°C consists of LiF, LixPFy, LixPOyFz, ROLi, and ROCO2Li.