This paper deals with an experimental evaluation regarding the real performance of lithium based energy storage systems for automotive applications. In particular real working operations of different lithium based storage system technologies, such as Li[NiCoMn]O2 and LiFePO4 batteries, are compared in this work from the point of view of their application in supplying full electric and hybrid vehicles, taking as a reference the well-known behavior of lead acid batteries. For this purpose, the experimental tests carried out in laboratory are firstly performed on single storage modules in stationary conditions. In this case the related results are obtained by means of a bidirectional cycle tester based on the IGBT technology, and consent to evaluate, compare and contrast charge/discharge characteristics and efficiency at constant values of current/voltage/power for each storage technology analyzed. Then, lithium battery packs are tested in supplying a 1.8kW electric power train using a laboratory test bench, based on a 48V DC bus and specifically configured to simulate working operations of electric vehicles on the road. For this other experimentation the test bench is equipped with an electric brake and acquisition/control system, able to represent in laboratory the real vehicle conditions and road characteristics on predefined driving cycles at different slopes. The obtained experimental results on both charge/discharge tests and driving cycles demonstrate the advantages of using lithium technologies, mainly in terms of their high efficiency, particularly at high current values. That represents a feasible solution to offer vehicle designers and users extended driving ranges and reduced recharging times.