This paper used foot-operated fitness equipment, a power generation system and a biomedical measurement system to construct a fitness system for fitness exercise, energy storage and diagnosing the user’s movement conditions. The diagnostic mode is that the rotation speed and output power of pedal-dynamo are converted into the mileage and calorie consumption of the user’s pedaling movement. Second, the biomedical measurement module can diagnose the user’s movement health status timely according to the heartbeat signals and the consumed calories while the user is in exercise. The diagnostic data were integrated using touch tablet PC and LabVIEW software for the image controlled man-machine interface. The user can observe the real-time motion state through the virtual instrument interface. The built-in program can diagnose the health status, and count the total generation and movement process after exercise.