Large-capacity rechargeable batteries for mobile devices are strongly required now, and the metal-air battery is currently considered as one of the most promising rechargeable batteries with a large capacity. In this study, oxygen reduction (ORR) and oxygen evolution reaction (OER) on mesoporous MnCo2O4 spinel was studied for rechargeable Zn-air battery. Comparing the prepared spinel oxides containing Fe, it was found that the ORR/OER activity of MnCo2O4 was reasonably high and stably cycled. Mesoporous MnCo2O4 was successfully prepared by using a hard template method with mesoporous silica (SBA-15) for an inorganic template. The obtained mesoporous MnCo2O4 showed the BET surface area of 108 m2/g and the average pore size of 2 nm. By increasing the surface area with mesoporous structure, overpotential of MnCo2O4 to ORR/OER was much decreased. Zn-air battery was prepared by using mesoporous MnCo2O4 as air electrode and 4 M KOH aqueous electrolyte, the stable discharge potential and capacity were exhibited at 1.05 V and 700 mAh/g-Zn, respectively. In addition, stable charge/discharge cycles were also sustained more than 200 cycles.