Abstract A graphite–vegetable oil-derived polyurethane was proposed as a composite electrode material for use in electroanalytical procedures. The advantages of such material include nonswelling during use in aqueous media, possibility of molding the final device into a variety of shapes, resistance to organic solvents allowing its use in nonaqueous systems, and easiness of incorporating modifiers in bulk. The preparation, characterization, and application of the bare and modified electrodes are described. The bare electrodes could be used in the determination of organic substances in a wide range of potentials without adsorption of analytes and its oxidation/reduction products. Modification with organofunctionalized silica and bismuth films allowed the simultaneous determination of metal ions in water and in ethanol fuel; molecularly imprinted polymers (MIPs) improved selectivity of the device; and biomimetic systems could also be incorporated in the electrode. Imprinting of the uncured composite permitted the confection of disposable screen-printed electrodes with or without modification.