 Anatase TiO2 is prepared via a facile and eco-friendly synthesis method where titanium tetra isopropoxide and sodium alginate are used as the titanium precursor and templating agent, respectively. Structural characterization of the prepared sample via X-ray diffraction and Raman spectroscopies confirm the formation of nanoparticles Titanium Oxide with anatase type structure without impurities. Further morphological characterization of the material showed the spherical shape of the particles. The prepared TiO2 has been studied as an anode material for lithium-ion batteries. TiO2 electrodes have delivered a reversible capacity of 266 mAh g−1, 275 mAh g−1 with coulombic efficiencies of 70%, 75% during the first cycle under C/10 current rate for TiO2 calcined at 300 °C and 450 °C, respectively. The activity of Ti4+/Ti3+ redox couple during the lithiation/delithiation process was evidenced using X-ray Photoelectron Spectrometry. The high capacity retention was maintained for 100 cycles. The prepared nanoparticles TiO2 has prodigious potential for large scale production of anode materials for lithium-ion batteries.