Biofuel cells are important to generate alternative energy. This paper evaluates the electroactivity of a carbon cloth platform containing immobilized multiwalled carbon nanotubes (MWCNTs), a NAD+-dependent alcohol dehydrogenase enzyme (ADH), poly-methylene green (poly-MG), and an anchoring agent (PAMAM dendrimer, modified NAFION (m-NAFION), or polypyrrole (poly-Pyr)). The PAMAM dendrimer is an excellent medium to achieve ADH immobilization on the carbon nanostructured platform. We obtained power density and open circuit potential (OCP) of 0.278±0.038mWcm−2 and 0.459±0.025V, respectively, for PAMAM modified with MWCNTs. This new anode configuration is potentially applicable for small energy devices.