In the present paper, the influence of carbon nanofiber on interlaminar fracture toughness of CFRP investigated using MMB(Mixed Mode Bending) tests. Vapor grown carbon fiber VGCF and VGCF-S, and multi-walled carbon nanotube MWNT-7 has been employed for the toughener of the interlayer on the CFRP laminates. In order to evaluate the fracture toughness and mixed mode ratio of it, double cantilever beam (DCB) tests, end notched fracture (ENF) tests and mixed mode bending (MMB) tests have been carried out. Boundary element analysis was applied to the CFRP model to compute the interlaminar fracture toughness, where extrapolation method was used to determine the fracture toughness and mixed mode ratio. The interlaminar fracture toughness and mixed mode ratio can be extrapolated by stress distribution in the vicinity of the crack tip of the CFRP laminate. It was found that the interlaminar fracture toughness of the CFRP laminates was improved inserting the interlayer made by carbon nanofiber especially in the region where shear mode deformation is dominant.