A polymer electrolyte membrane fuel cell (PEMFC) stack has been operated under low humidity conditions during 1000h. The fuel cell characterisation is based both on polarisation curves and electrochemical impedance spectra recorded for various stoichiometry rates, performed regularly throughout the ageing process. Some design of experiment (DoE) techniques, and in particular the response surface methodology (RSM), are employed to analyse the results of the ageing test and to propose some numerical/statistical laws for the modelling of the stack performance degradation. These mathematical relations are used to optimise the fuel cell operating conditions versus ageing time and to get a deeper understanding of the ageing mechanisms. The test results are compared with those obtained from another stack operated in stationary regime at roughly nominal conditions during 1000h (reference test). The final objective is to ensure for the next fuel cell systems proper operating conditions leading to extended lifetimes.