Plasticized chitosan-proton conductor polymer electrolyte films were prepared by dissolving chitosan powder, ammonium nitrate (NH4NO3) salt and ethylene carbonate (EC) plasticizer in acetic acid solution. The highest conductivity of the chitosan-salt with 40wt.% NH4NO3 in the film at room temperature was 8.38±4.11×10−5 Scm−1 and this increased to 9.93±1.90×10−3 Scm−1 with 70wt.% EC. Batteries with a configuration of: Zn+ZnSO4·7H2O/18wt.% CA-12wt.% NH4NO3-70wt.% EC/MnO2 provided an open-circuit voltage of 1.56±0.06V. The discharge characteristics using a 1mA constant current demonstrated a capacity of 17.0±2.6mAh. The internal resistance was 29.8±5.1Ω. While the highest power density was 8.70±1.91mWcm−2.