Li4Ti5O12/C nano-particles is synthesized at 800°C, and investigated by XRD, SEM, TEM and HRTEM, and the carbon in the composite is detected by Raman spectroscopy. Experimental results indicate that compared with those synthesized at 600, 700 and 900°C and pure Li4Ti5O12 the Li4Ti5O12/C nanoparticles synthesized at 800°C remarkably enhances the rate performance and cycling performance of lithium-ion batteries. Lithium-ion batteries with nanocrystalline Li4Ti5O12 used as anode exhibit an excellent reversible capacity of 169, 141, 132, 106 and 82mAhg−1, at the current density of 1C, 10C, 20C, 40C and 80C, respectively.