Electronic cigarettes (ECs) have recently become very popular among the population due to the various flavors available for vaping products. Consequently, manufacturers are trying to attract more users through the addition of various additives including nicotine, new flavors, and aromas. However, the inhalation of unknown and untested chemicals may cause health problems. Therefore, more clinical reports need to be collected for standardization of ECs for their effective regulation. However, detailed compositional information for EC additives is not commonly available. The aim of this review is, therefore, to study the state-of-the-art methods that can be employed for the quantitative analysis of the composition of electronic cigarettes with respect to these additives and flavors along with the basic cigarette ingredient like nicotine (both before and after consumption). In addition, efforts have also been made to address the clinical impacts of vaping EC and its additives on both the organ and cellular level. It was found that the effect of flavorings in EC is clearly associated with pathogenicity at the molecular level and need of standardization of the usage of EC and its flavorings is urgently needed.