Municipal solid waste management (MSWM) is one of the major environmental issues in Tunisian cities. Rapid growth in urbanization and population rates and the changes in people’s lifestyle have prompted a dramatic increase quantity and a significant shift in the composition of municipal solid waste. There is insufficient data concerning the quantities and the composition of waste streams along with the absence of a comprehensive complete overview and a wider perspective of MSWM potential that provides detailed information at region and city level. As a result, it is still impossible for the scientific community and the authorities to provide synergetic schemes to tie the problems of MSWM with how to integrate economically feasible and environmentally sustainable practices holistically. In the present study, an attempt has been made to provide a comprehensive overview of MSW, through a qualitative (compositional) and quantitative (parametric) characterization of the generated total waste generated in Tunisian cities. A 1-year research survey was conducted in seven regions in Tunisia (Great Tunis, Northeast, Northwest, Midwest, Mideast, Southwest, and Southeast) that cover the 24 provinces of the country. Collected samples revealed that the distribution of waste by region was defined by the region’s demographic, economic, and industrial status. Approaches of possibly more efficient procedures that can be undertaken to improve MSW collection are discussed. At a final stage and based on the potential of biogas calculated in the seven regions, we suggest that the scientific community and the authorities should introduce applicable schemes to valorize MSW through generating biogas as a renewable energy. 