Lead (Pb) is a common cause of heavy metal poisonings in cattle. Sources of Pb on farms include crankcase oil, machinery grease, batteries, plumbing, and paint chips. Consequently, consumption of Pb from these sources may negatively impact animal health and Pb may be inadvertently introduced into the food supply. Therefore, the scope of poisoning incidents must be clearly assessed and sources of intoxication identified and strategies to mitigate exposure evaluated and implemented to prevent future exposures. Stable isotope analysis by inductively-coupled plasma mass spectrometry (ICP-MS) has proven itself of value in forensic investigations. We report on the extension of Pb stable isotope analysis to bovine tissues and profile comparisons with paint chips and soils collected from an affected dairy farm to elucidate the primary source. Pb occurs naturally as four stable isotopes: 204Pb, 206Pb, 207Pb, and 208Pb. Herein a case is reported to illustrate the use of 207Pb/206Pb and 208Pb/206Pb ratios to link environmental sources of exposure with tissues from a poisoned animal. Chemical Pb profiling provides a valuable tool for field investigative approaches to Pb poisoning in production agriculture and is applicable to subclinical exposures.