Seeds of Glinus lotoides L. (Molluginaceae) are used traditionally in the treatment of tapeworm infestation in Ethiopia. Previous studies on its anthelmintic activities confirmed its traditional claims, but data on safety profile were lacking. To this effect, single and repeated dose oral toxicities of the methanolic extracts of seeds of Glinus lotoides were conducted in rats. Doses of 0, 1000 and 5000mg/kg of crude extract of Glinus lotoides were employed in single dose toxicity studies, while doses of 0, 250, 500, and 1000mg/kg were used in repeated toxicity studies. In the single dose toxicity test, oral administration of 5000mg/kg of Glinus lotoides produced mortality in two females and one male on day 4. No significant differences in body and organ weights were observed between controls and treated surviving animals. Moreover, both gross and microscopic examinations of organs did not show detectable differences between controls and treated animals of both sexes. In repeated dose toxicity studies, no mortality was observed when varying doses of the extracts were administered per day for a period of 28 days. There were no significant differences in body weight, absolute and relative organ weights between controls and treated animals of both sexes. Hematological analysis showed no differences in most parameters examined. In the biochemistry parameter analysis, no significant change occurred. Pathologically, neither gross abnormalities nor histopathological changes were observed. These finding suggest that none of the organs appeared to be target and the data could provide satisfactory preclinical evidence of safety to launch clinical trial on standardized formulation of plant extracts.