Roger W. Giese With many researchers concentrating on a cure for
cancer, there are some focusing on detecting genetic
predispositions early enough for preventative measures. The
Environmental Cancer Research Program (ERCP) is one such place.
Specific research is being done on DNA adducts, however the
bigger bulkier adducts are having low yields. This research
focused on optimizing the conditions of the enzyme used to
detach the DNA adducts from DNA. The data showed that that
extended incubation of the enzyme at 45??C does not diminish the
enzymatic rate, and increasing the concentration of Mg2+ in the
buffer results in a lower enzymatic rate after extended
incubation at 55 C. DNA Adducts, Enzyme rate, Phosphodiesterase
I Calhoun, Shawna, "Optimization of exonuclease digestion for
isolation of DNA adducts" (2007). Honors Junior/Senior Projects.
Paper 34. http://hdl.handle.net/2047/d10006564 Click button
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