Light Scattering System NanoBiophysics Core Facility has a full
set of Light Scattering equipment from Wyatt including
Multiangle Light Scattering (MALS) device, Dynamic Light
Scattering (DLS) device, and HPLC system (Agilent) linked to
MALS. Light scattering is a non-invasive technique for
characterizing macromolecules and a wide range of particles in
solution. In contrast to most methods for characterization, it
does not require outside calibration standards. In this sense it
is an absolute technique. Wyatt Technology instruments make two
different types of light scattering measurements for absolute
molecular characterization: * Classical light scattering: Here,
the intensity of the scattered light is measured as a function
of angle. For the case of macromolecules, this is often called
Rayleigh scattering and can yield the molar mass, rms radius,
and second virial coefficient (A2). For certain classes of
particles, classical light scattering can yield the size, shape,
and structure. * Quasi-elastic (QELS) o