PREPARATION OF INOCULUM Preparatory to the test, inoculate the
surface of a suitable volume of solid agar medium from a
recently revived stock culture of each of the specified
microorganisms. The culture conditions for the inoculum culture
are described in Table 2 in which the suitable media are
Soybean–Casein Digest or Sabouraud Dextrose Agar Medium . To
harvest the bacterial and C. albicans cultures, use sterile
saline TS, washing the surface growth, collecting it in a
suitable vessel, and adding sufficient sterile saline TS to
obtain a microbial count of about 1 × 108 colony-forming units
(cfu) per mL. To harvest the cells of A. niger, use sterile
saline TS containing 0.05% of polysorbate 80, and add sufficient
sterile saline TS to obtain a count of about 1 × 108 cfu per mL.
Alternatively, the stock culture organisms may be grown in a
suitable liquid medium (i.e., Soybean–Casein Digest Broth or
Sabouraud Dextrose Broth) and the cells harvested by
centrifugation, then washed and resuspended in sterile salin