1. DVBD Survey for Arthropod-Borne Viruses in Uganda B. R.
Miller We are conducting adult mosquito trapping in pristine
habitats, at the human-animal interface, farms and ranchland in
Uganda. Captured mosquitoes are frozen and returned to the
Uganda Virus Research Institute where they will be shipped to
CDC Fort Collins to be sorted by species and tested for the
presence of arboviruses in Vero cell cultures. Viral isolates
will be identified using NIH grouping fluids, RT-PCR and
sequencing in CDC-Ft. Collins. Unknowns will be further
characterized in Fort Collins, Colorado in a BLS-3 enhanced
laboratory with HEPA air filtration with investigators wearing
personal protective suits and respirators. Low passage virus
isolates will be made available to other investigators by
placing isolates in WHO Collaborating Centers. This information
will be useful in determining arboviral activity in different
ecological zones in Uganda and because viral isolation will be
done, new viral threats will also be discovered. Crab