Debate and discussion of any biological questions not pertaining
to a particular topic. 3 posts • Page 1 of 1 A fluorescence
microscope can reveal whether there are detectable
concentrations of fluorescent material in a sample and how the
material is distributed in the sample. However, to check for
different excitation wavelengths and emission colors, you must
switch part of the optics in the microscope. This is easy in
most fluorescence microscope as you slide a different "cube"
into the light path. A fluorescence microscope cube contains a
filter for the excitation light that lets "usually" a fairly
narrow band of wavelengths through to reach the sample, a
dichroic mirror used to split excitation and emitted light,
sending them along different paths and an emission filter which
helps prevent excitation light from reaching the observer's eye.
The technique is interesting and useful because there are many
fluorescent probes which can be applied to a sample to reveal
different structures. Sometimes they are sm