Complete Antibiotic Production & Sensitivity Testing Examine the
Antibiotic plates and look for evidence of a zone of inhibition
(no growth or reduced growth) of any of the "control" organisms
in an area near the putative antibiotic producer's colonial
growth. Evidence of antibiotic production should appear as a
measurable zone of inhibition (section of a circle of no growth
or reduced growth compared to the growth seen on the control
plate). The size of the zone of inhibition is indicative of the
diffusion potential of the antibiotic and/or an indication of
how sensitive the test organism is to the secreted inhibitor.
Compare your results to other tested isolates in your lab
section. Think about why an antibiotic might work differently on
a Gram positive vs. a Gram negative organism or between two
bacteria that are both Gram positive or Gram negative. If you
have positive antibiotic producers or AI producers, or cool
interactions please show them to your lab instructor so they can
be shared with the class an