Figure 1. Parallel PCR amplification of PARV4-like (A) and PPV
(B) by using purified DNA from clotting FVIII preparations. The
results of this PCR usually suggested a higher PARV4 load
despite the higher efficiency of the PPV PCR (J. Szelei and P.
Tijssen, unpub. data). This finding was confirmed with the
quantitative MIMIC PCR method for PPV (11). Numbers indicate
different lots of FVIII prepared in 1:1994A, 2:1994B, 3:1996A,
4:1996B, 5:1999, 6:2000A, 7:2000B, 8:2001A, 9:2001B, 10:2001C,
11:2001D, and 12: DNA marker (1-kb ladder; Invitrogen, Carlsbad,
CA USA). PARV4, parvovirus 4; PPV, porcine parvovirus; FVIII,
factor VIII.