Isolate small RNA and DNA from plasma and serum. The NucleoSpin
miRNA Plasma procedure is a simple and fast method to isolate
small RNA >15 nt, such as micro RNA (miRNA), from serum or
plasma. The DNA can be isolated as well or digested on-column to
eliminate interference of miRNA genes with downstream
applications. This proven, efficient silica membrane-based
procedure isolates total RNA and DNA from plasma without the
need to resort to the cumbersome phenol/chloroform extraction or
a time-consuming proteinase digest. The sample material is
denatured in Lysis Buffer MLP. The protein is then precipitated
by Protein Precipitation Buffer MPP and pelleted by
centrifugation. After the removal of protein, the binding
conditions for nucleic acids are adjusted by adding isopropanol,
allowing small RNA and DNA to bind to the NucleoSpin miRNA
Column. Optionally, DNA can be removed by an on-column rDNase
digest. The remaining nucleic acids are washed and eluted with
minimal amounts of RNase-free water.