Fed-batch and perfusion culture are the two dominant modes of
operation for mammalian-cell-culture based processes, especially
for the production of glycosylated proteins required in large
amounts. This article provides an economic comparison for the
production of a typical glycosylated protein using the fed-
batch, concentrated fed-batch (CFB), and concentrated perfusion
(CP) technologies. The CFB and CP processes are based on the ATF
System, a platform technology developed by Refine Technology for
biologics production. The market for protein-derived products
has grown significantly in the past decade and continues to
accelerate at a rapid rate. As more recombinant therapeutic
proteins enter development and get through the approval phase,
more efficient large-scale production of such proteins is
necessary to meet the surging demand. Fed-batch and perfusion
culture are the two dominant modes of operation for mammalian-
cell-culture based processes, especially for the production of
glycosylated proteins required