sib at brown.edu Mon Oct 30 09:52:15 EST 1995
<Pine.SUN.3.91.951030083545.514B-100000 at
london.biology.lsa.umich.edu>, ldnum at BIOLOGY.LSA.UMICH.EDU
(Larry D Nooden) wrote: > My vote is for phaeophytin. Basically
what happens is that acids > released from the vacuoles during
homogenization of the cells cause the > Mg+2 in chlorophyll to
be replaced with H+, so you will need to use a > strong buffer
ca. pH 7. See Canfield et al. in Annals of Botany 75: 143- >
(1995) for discussion and references. Although pheophytin is
likely to be formed during extraction of acidic plant cells, I
doubt that it is the pigment under consideration, because
pheophytin is more hydrophobic than chlorophyll and would
therefore run ahead of chlorophyll rather than stay behind. More
information about the Plant-ed