priciples behind nucleic acid isolation - need this for
additional lecture information (Aug/02/2005 ) I really need some
information on the actual principles behind the following
procedures to isolate nucleic acids: alkaline lysis , phenol
chloroform extrcation , cesium chloride and silica dioxide. What
actually happens? why it happens ...the chemistry details
........... I am not going to answer all your questions, but the
basis of CsCl centrifugation is differences in density between,
RNA, DNA and protein. CsCl is used because it forms a
spontaneous gradient when centrifuged of the right density to
separate the above three fractions. DNA sequencing software try
to ask specfic questions.... IT would be easier to answer to a
particular point that doing all the chemistry of alkaline lyisi
for ex... Search at google