Specialized Microscopy Techniques Modern microscopists and
optical engineers have developed a wide spectrum of useful
techniques designed to aid in contrast enhancement, provide
better observation, and assist in the collection of
photomicrographs and digital images of a wide variety of
specimens. This section of the Molecular Expressions Microscopy
Primer describes many of these techniques in detail. Contrast in
Optical Microscopy - With the assistance of Dr. Robert Hoffman,
we review the problems of contrast enhancement with both
amplitude and phase specimens and review techniques that have
been developed to assist with specimen contrast. Darkfield
Microscopy - Oblique illumination can be used to increase the
visibility of specimens lacking in sufficient contrast that are
difficult to observe with standard brightfield microscopy. This
section discusses various aspects of the theory and practice of
condenser design and other important concepts in both
transmitted and reflected light darkfield microscopy. Diff