Date of this Version Great Salt Lake-biotype brine shrimp cysts
were added to 440-1 saltwater cultures maintained at 20° ,25° ,
and 30° C. A micronized corn-byproduct was added each day as
food. Planktonic bacteria found during growth of the brine
shrimp were enumerated on Plate Count Agar (Difco). From the 25°
and 30° brine shrimp cultures, a total of seven prevalent
bacterial types (five gram-negative and two gram-positive) were
isolated from throughout the 12-day growth cycle. Eight
antimicrobial agents were tested against these bacteria, with
chloramphenicol the most effective antibacterial agent overall,
followed by erythromycin and carbenicillin. The bacterial
density of the culture medium upon cyst addition was 106 colony
forming units (cfu)/ml. The density increased similarly in all
cultures from day 1 to day 3, and continued to increase in both
the 25°- and 30°- cultures to between 108 and 109 cfu/ml at the
end of day 12, at which time many brine shrimp had reached
sexual maturity. However, the bacte