Campylobacter coli is the most common Campylobacter species
found in pig(95%),but the ability of this bacterium to survive
in swine manure as well as the potential for causing human
illness are poorly understood. We present here laboratory-scale
experiments to investigate the effect of temperature on the
survival of C.coli in spiked swine manure samples at
temperatures from 4 to 52˚C. The survival of C.coli during
storage for 30 days was studied by three different methods:
bacterial culture(plate counting),DNA qPCR, and mRNART-qPCR.The
results indicate that C.coli could survive in swine manure up to
24 days at 4˚C. At higher temperatures,this bacterium survived
only 7 days(15˚C)or 6 days(22˚C)of storage. The survival of
C.coli was extremely short(few hours)in samples incubated at 42
and 52 ˚C. The results from the RT-qPCRmethodwereconsistentwitht
hedatafromthebacterialculturemethod, indicating that it detected
only viable C.colicells,thus eliminating false-positive
resulting from DNA from dead C.coli cells. Th