Chromatin Immunoprecipitation (ChIP) Chromatin
immunoprecipitation (ChIP) is a method used to determine the
location of DNA binding sites on the genome for a particular
protein of interest. This technique gives a picture of the
protein-DNA interactions that occur inside the nucleus of living
cells or tissues. Determining how proteins interact with DNA to
regulate gene expression is essential for fully understanding
many biological processes and disease states. Microarray-based
ChIP studies are restricted to a fixed number of probes
Currently, the most common method for genome-wide analysis of
DNA-binding proteins is Chip-on-chip (ChIP-chip), which combines
chromatin immunoprecipitation with DNA microarrays. The major
limitation of this hybridization-based method is that
microarrays are restricted to a fixed number of probes, thereby
introducing bias. ChIP-Seq with the SOLiD™ System SOLiD™ System-
based ChIP-Sequencing (ChIP-Seq) overcomes the limitation of
microarrays by combining chromatin immunoprecipitation