Quantitative reverse transcriptase PCR (QRT-PCR or qRT-PCR) is a
PCR technique used to determine the amount of cDNA in a sample.
It is the most commonly used form of quantitative PCR (qPCR).
This technique is also called real-time reverse transcriptase
PCR. Comparison of normalisation methods There is an ongoing
debate what is the best way to normalise qPCR data. Reference
genes are the most common method, although single unverified
reference genes invalidate the qPCR data generated. Total RNA,
ribosomal RNA, and genomic DNA have been suggested as
alternative methods. Most common method. Best practise is a
panel, e.g.  not just a single reference gene and including data
on suitability as reference genes. Often housekeeping gene is
used here instead of reference gene but the term is poorly
defined and can be misleading. Total rRNA  , or total RNA.
Drawback: rapidly dividing cells will have more rRNA and
different rRNA/mRNA ratio which will complicate comparison;
difference in cDNA synthesis not taken into acco