etd AT Indian Institute of Science > Division of Biological
Sciences > Molecular Biophysics Unit (mbu) > Please use this
identifier to cite or link to this item: |Title: ||Design And
Isolation Of Temperature Sensitive Mutants Of Gal4 In Yeast And
Drosophila| |Authors: ||Mondal, Kajari| |Advisors:
||Varadarajan, Raghavan| Temperature Sensitive (ts) Mutants
|Submitted Date: ||Dec-2006| |Series/Report no.: ||G21112|
|Abstract: ||Genomic and proteomic investigations have yielded,
and continue to produce, a large amount of information about
genes and their protein products. In contrast, the evidence
bearing on physiological roles of specific proteins is much more
scarce. To address the functional part of biological inquiry,
one would like to perturb, at will and selectively, the function
of any protein of interest in vivo and to analyze the resulting
phenotypic effects, thereby probing the protein’s role in a
cell. Ideally, a method for doing so should be applicable both
to individual gene products and to a large