- Pfu is a thermostable DNA polymerase that can be used for PCR.
- Pfu has proof reading activity which makes it more accurate
than for example Taq. - Pfu does not exhibit 5' -> 3'
exonuclease activity, which prevents degradation of the primers
in mutagenesis methods based on ligation of primers or elongated
primers. - Pfu is slower than Taq and one should calculate 2 min
per 1000 bp (1 min for Taq) (see Promega Pfu description). -
Elogation speed (bp/sec) tends to be lower than for example Taq.
- Build-in "Hotstart", because it's only active at high
temperature, unlike Taq which has some activity at lower
temperatures too - Can degrade oligonucleotides, causing
smearing (on gel).