Posted 14 September 2010 - 02:49 PM We know that a six base pair
sequence AGAATA occurs 1 time in 4096 nucleotides [ (1/4)^6 =
4096 ] correct? Let's say we have a DNA library that is 50,000
base pairs in total (small library of 50 clones to keep numbers
small) it should occur roughly ~12 times [ 50000/4096 = 12.2 ]
with 100% probability? 1. What is the probability that it would
occur twice? How about 5 times? 2. What is the probability it
would occur once in a single clone from this library if the
clone is 500 bp long? What about twice? 3. And finally, let's
say AGAATA occurs 3 times in one 500 bp clone. Is this
significant? What is the equation? I wanted to know basically,
"This six base pair sequence has an X-percent probability of
occurring within the clone, and an x-percent probability of
occurring in the whole library. Thanks. Posted 14 September 2010
- 06:49 PM Posted 14 September 2010 - 07:41 PM Let's assume it's
25% each. And I can replace this sequence with any sequence. I'm
just looking on how to do