- High-throughput SuperSAGE for digital gene expression analysis
of multiple samples using next generation sequencing (2010) - We
established a protocol of the SuperSAGE technology combined with
next-generation sequencing, coined “High-Throughput (HT-)
SuperSAGE”. SuperSAGE is a method of digital gene expression
profiling that allows isolation of 26-bp tag fragments from
expressed transcripts. In the present protocol, index (barcode)
sequences are employed to discriminate tags from different
samples. Such barcodes allow researchers to analyze digital tags
from transcriptomes of many samples in a single sequencing run
by simply pooling the libraries. Here, we demonstrated that HT-
SuperSAGE provided highly sensitive, reproducible and accurate
digital gene expression data. By increasing throughput for
analysis in HT-SuperSAGE, various applications are foreseen and
several examples are provided in the present study, including
analyses of laser-microdissected cells, biological replicates
and tag extraction using d