What is High Performance Liquid Chromatography Testing? Do you
use high performance liquid chromatography testing for your
products? Do you want to learn more about this popular test
method? High performance liquid chromatography , also known as
HPLC is one form of liquid chromatography frequently used in
analytical chemistry and biochemistry. This test method is used
to separate components in a mixture by performing a variety of
chemical interactions between the substances being analyzed and
the chromatography column. HPLC instruments consist of a
reservoir of mobile phase, a pump, an injector, a separation
column and a detector. The chemist separates the various
compounds by injecting an aliquot of the sample mixture onto the
column. The various parts in the mixture will pass through the
column at varying rates because of the differences in their
partitioning behavior between the mobile liquid phase and the
stationary phase. HPLC has been around since the early 1970s and
is a very popular method of analysis