Preserving RNA Before Isolation Appropriate storage of cells or
tissue samples prior to RNA isolation is critical for
preservation of intact RNA within those samples. RNAlater is an
aqueous solution designed specifically to maintain RNA integrity
during storage of fresh tissue and cell samples. Below are some
commonly asked questions regarding RNAlater® 1) How do I use
RNAlater to store my tissue/cell sample? - For fresh tissue,
simply cut samples to a maximum thickness of 0.5 cm in any one
dimension and submerge in 5 volumes of RNAlater. - Cultured
cells should be pelleted, resuspended in a small volume of PBS,
then mixed with 5-10 volumes of RNAlater. - Once in RNAlater,
samples can be stored for up to 1 day at 37°C, 1 week at 25°C, 1
month or more at 4°C, and long-term at -20°C or -80°. 2) Does
storing samples in RNAlater have advantages over freezing
samples on dry ice or in liquid nitrogen? 3) Has RNAlater been
tested with my tissue or with blood samples? 4) Which downstream
applications can be used with