GloMax®-Multi Jr Method for DNA Quantitation Using Hoechst 33258
- Comments & Ratings Quantitation of DNA is an important step
for many practices in molecular biology. Common techniques that
use DNA, such as sequencing, cDNA synthesis and cloning, RNA
transcription, transfection, nucleic acid labeling (e.g., random
prime labeling), etc., all benefit from a defined template
concentration. Failure to produce results from these techniques
sometimes can be attributed to an incorrect estimate of the DNA
template used. The concentration of a nucleic acid most commonly
is measured by UV absorbance at 260nm (A260). Absorbance methods
are limited in sensitivity, however, due to a high level of
background interference.