Living Colors PAmCherry is a photoactivatable fluorescent
protein (PAFP) derived from the red fluorescent protein mCherry.
PAmCherry is non-fluorescent until it is exposed to 350–400 nm
light. By selecting which cells or cellular regions to activate,
you can track cells, organelles, or proteins of interest against
a dark background using this photoactivatable fluorescent
protein. The activated PAmCherry excitation maximum is 564 nm,
and the emission maximum is 595 nm, which allows you to
visualize and monitor photoactivated PAmCherry with the same
filter sets used for other red fluorescent proteins, such as
DsRed variants and mCherry. Photoactivatable fluorescent
proteins such as PAmCherry are particularly useful for
determining protein half-life and protein transport pathways,
because molecules synthesized after activation are not able to
fluoresce. Therefore, what you observe is a snapshot of the
protein molecules that were present at the time of activation.
PAmCherry subcellular localization vectors are av