Transcription factors are recognized as important components of
signaling cascades controlling all types of normal cellular
processes as well as response to external stimulus, conditions
of disease, drug treatment, and more. However, comparatively few
known transcription factor binding sites have been
experimentally characterized relative to the total number of
binding sites expected. With the advance of next generation
sequencing (NGS) technologies, namely ChIP-seq, researchers now
have the ability to study transcription factor binding sites on
a genome-wide scale. This webinar discusses the advantages that
ChIP-seq technologies offer over traditional binding site
analysis and demonstrate a typical application of ChIP-seq
through the analysis of TAL1 intervals in two haematopoietic
lineages using the ExPlain™ analysis system. - Application of
ChIP-seq to the discovery of transcription factor binding sites
and composite modules - Strengths and weaknesses of ChIP-seq
compared to traditional binding site analys