Illumina Genome Analyzer Author: Rodney E. Shackelford, D.O.,
Ph.D., Tulane University (see Reviewers page) Revised: 13
February 2011, last major update February 2011 Copyright: (c)
2008-2011, PathologyOutlines.com, Inc. ● The Illumina Genome
Analyzer, similar to the 454 FLX Sequencer, begins by
constructing a sequence mixed library of DNA fragments of up to
several hundred bp ● Adaptor-sequences with forward and reverse
PCR primers are attached to each end of these DNA fragments ●
The adaptor-linked sequences are then amplified by bridge PCR,
where both the forward and reverse PCR primers are tethered to a
solid substrate by a flexible linker ● During PCR, the amplified
sequences remain immobilized and clustered in one location on
the array; eventually there are 1,000 to 1 million clustered
amplicons, sufficient for reporting incorporated bases as the
required signal intensity ● Several million different clusters
can be amplified and distinguished at different locations within
eight independent lanes that ar