Nucleoside-diphosphate (NDP) Lyophilisates Nucleoside
diphosphates (NDPs) are pivotal molecules in a plethora of
cellular processes. The exothermic hydrolysis of ATP yielding
ADP and inorganic phosphate (Pi) is the major source of
biological energy, and ATP/ADP as well as GTP/GDP are involved
in the signal transduction pathways by kinases and G-Proteins.
NDPs can be converted to their triphosphates (NTP) by kinase-
mediated enzymatic attachment of one additional phosphate while
NTPs in combination with a phosphatase are converted back to
NDPs (Fig. 1). NDPs are widely used in signal transduction
research as cofactors and serve as templates for chemical or
enzymatic synthesis of non-natural analogues. Dynamic
equilibrium of nucleoside diphosphate (NDP) and its triphosphate
(NTP)