1. The antibiotic "Cordycepin" is shown below. Of what
nucleoside is it an analog? Why does it inhibit bacterial RNA
synthesis? 2. For the E. coliTyr Promoter: 5’
CAACGTAACACTTTACAGCGGCGCGTCATTTGATATGATGCGCCCCGCTTCCCGATA 3’ 3’
GTTGCATTGTGAAATGTCGCCGCGCAGTAAACTATACTACGCGGGGCGAAGGGCTAT 5’
Indicate the -10 region, the -35 region, and the initiating
nucleotide on the sense strand. 3. Explain the effects of Gyrase
mutants and of Topoisomerase I mutants on transcription of
plasmids in E. coli. 4. An experiment is done in which a certain
RNAPII is incubated with an inhibitor of RNA chain elongation:
No 32P was found in the transcribed RNA. What might you conclude
from this finding? 5. Draw the structures of the side chains
that correspond to the following histone modifications, and
explain how each modification might affect replication or
transcription: a) Acetylation of Lys b) Phosphorylation of Ser
c) Phosphorylation of His d) Methylation of Lys e) Methylation
of Arg 6. Explain the chemical reactions of the 5'-cap