PCR of ADA Mutations - Prepare ADA primers in order to conduct a
polymerase chain reaction (PCR) - Run HRP Luminol Assay -
Assigned Primer= ADA K110A F (forward primer) - Sequence Issue
number: 87635082 - Sequence: ATT TGG CCA AAT GGG CAG TTA TTG AA
- TM= 65.5°C - MW= 16833.0 g/mol - 33.4 nm= .56 mg ( amount of
present plasmid) Concentration of plasmid needed for experiment=
100ng/μL .56mg= 5600 ng 5600 ng/μL x V1= 100ng x 1000μL - (The
container containing the plasmid only had room for 1mL of water
to be added- as a result, a dilution needed to be done in order
to get the appropriate concentration. 1mL of water was added to
dissolve the plasmid prior to the dilution V2= 17.857μL is the
amount of plasmid solution pipetted out in order to get a
concentration of 100ng/μL - 1mL of deionized water was added to
.56mg of ADA K110A F - 17.86μL of this solution was pipetted out
and added to a centrifuge tube. 982.14μL of autoclaved water was
then added to this sample in order to dilute it to 100ng/μL. -
Next, 2 50μL