Posted 25 April 2012 - 12:20 AM I want to isolate thiobacillus
species from soil. I need to add elemental sulfur to culture
media but it is not soluble.what can I do? Posted 25 April 2012
- 06:37 AM Posted 13 June 2012 - 12:01 AM Elemental sulfur won't
solubilize, but in these media it is not expected to happen...
as note from DSMZ: making it unusable by microorganisms. Hence,
it is important to sterilize the sulfur powder separately from
the liquid medium. This can be achieved by filling screw cap
tubes (18 x 100 mm, borosilicate glass) with sulfur powder
(approx. 2/3 volume) to which 1 or 2 drops of distilled water is
added. The screw cap should not be tightly closed. The tubes are
heated in a water bath to 90-100 °C for 3 hours on each of 3
successive days. The sterilized sulfur can be stored at room
temperature in the dark for several months. They state that the
sulfur remains at the liquid-air-interface in freshly prepared
sterile medium while as a result of growth most of the sulfur
gets coated by bacte