Pest science and management International Rice Research Notes
Seeds of rice variety MUT 7029 were soaked in water for 4 d, and
thewater was changed daily. After 4 d, 30 sprouted seeds were
each seeded inearthen pots containing 1 kg of soil. Ten pots
were used as replicates.Immediately after sowing, a nematode
suspension containing 3,000–5,000 activesecond-stage juveniles
(J2) was inoculated into each pot. The pots were wateredto field
capacity. Within 6–8 h, the radicles developed; this was
followed bypenetration of the active J2. One gall per radicle
was formed 2 d after inoculation.To determine life-cycle
duration, the first root galls of the 10 seedlingswere sampled
daily until the eggs and the J2 were observed. Each day, the
rootsystems of these seedlings, one from each pot, were gently
removed, thoroughlywashed, and stained with acid fuchsin in
lactophenol following a methoddescribed by Daykin and Hussey
(1985). The stained roots were then transferredinto Petri dishes
and the first root galls were cut an