Trophic effect of adipose tissue derived stem cells on porcine islet cells.
BACKGROUND Adipose tissue derived stem cells (ADSCs) which are widely known as multipotent progenitor cells, release several cytokines that support cell survival and repair. The aim of this study was to investigate whether ADSC secreted molecules could induce a trophic effect in pancreatic islet culture conditions in vitro. MATERIALS AND METHODS We cocultured porcine islet cells with ADSCs using a transwell system for 48 h and evaluated the viability of islet cells. We also determined the concentration levels of cytokines and insulin in the supernatant of the culture medium. We used anti vascular endothelial growth factor (VEGF) and anti interleukin (IL) 6 receptor antibodies to investigate the effect of VEGF and IL 6 on islet cells. RESULTS ADSCs improved the viability of islet cells in the absence of cell cell contact (P 0.05) VEGF and IL 6 levels in the culture medium increased when islet cells were cocultured with ADSCs (P 0.05) Furthermore, inhibition of VEGF decreased the viability of islet cells (P 0.05) however, inhibition of IL 6 did not affect islet cell viability. CONCLUSIONS These results suggested that trophic factors, particularly VEGF, secreted by human ADSCs enhanced the survival and function of porcine islet cells.
The Journal of surgical research