ANTIMICROBIAL AND STABILITY STUDY OF BIOSURFACTANT PRODUCED FROM Pseudomonas aeruginosa PBS29
The biosurfactant production using the test strain Pseudomonas aeruginosa PBS29 performed using acid precipitation followed by solvent extraction. The effectiveness of various solvents such as Diethyl ether, Chloroform/Methanol (2:1 ratio) Dichloromethane, Ethyl acetate and Acetone were evaluated and confirmed that Chloroform/Methanol as ideal solvent comparatively. The CMC value of the biosurfactant was found to be 60 mg/ml. The extracted biosurfactant showed its stability over a wide range of temperature, pH, sodium chloride concentration, Foaming activity and effect of metal ions such as lead and zinc. Alkaline pH showed a higher stability than the acidic pH. The effect of temperature on the biosurfactant found to stable till 100 oC. Emulsification activity has not been affected till 20 Sodium chloride concentration.