Optimized Production of Lentiviral Vectors for CAR T Cell.
Advances in the use of lentiviral vectors for gene therapy applications have created a need for large scale manufacture of clinical grade viral vectors for transfer of genetic materials. Lentiviral vectors can transduce a wide range of cell types and integrate into the host genome of dividing and nondividing cells, resulting in long term expression of the transgene both in vitro and in vivo. In this chapter, we present a method to transfect human cells, creating an easy platform to produce lentiviral vectors for CAR T cell application.
Methods in molecular biology