Chemicals and Antioxidant Activity of Ethanol Leaf Extract from Pandanus amaryllifolius Roxb. Cultivated in Salinity Soil in Ban Donman Village, Maha Sarakham, Thailand
The leaves of Pandanus amaryllifolius Roxb. have a strong aroma and are used as flavoring for various food products. They are also widely used in folklore and traditional medicine in rural areas of Thailand. In this research, P. amaryllifolius leaves were obtained from local cultivation in Ban Donman village in Maha Sarakham Province, Thailand. 95% ethanol extract of P. amaryllifolius leaves (EEPL) was investigated in order to determine its natural potential. Chemical composition of EEPL was analyzed using gas chromatography coupled with mass spectrometry (GC MS) Twenty six compounds were identified. Elemental analyses of EEPL employed energy dispersive X ray (EDX) microanalysis, where six elements were identified. The amount of total phenolic compound (TPC) by Folin Ciocalteu method in the EEPL was 5 6 .4 6 0.56 mg GAE/g dry weight. The amount of total flavonoid content (TFC) by AlCl3 method was 1 2 0 .6 2 7 .3 6 mg QCE/g dry weight. Radical scavenging activity was determined from IC50 using DPPH radical scavenging method. 50% inhibition concentration (IC50 values, mg/ml) of standard ascorbic acid was 0.97 0.01 mg/ml, which was more potent than that of EEPL (12.57+ 0 4 5 mg/ml) According to the correlation coefficient, there was a positive correlation between TPC and IC50. Additionally, it was revealed that the solvent in the extraction affected the Vol.10 No.1 (2020) 194 206 www.sci.rmutt.ac.th/stj Online ISSN 2229 1547 Sci. Tech. RMUTT J. Vol.10 No.1 (2020) 195 amount of TPC, TFC and IC50. This study demonstrates that the 95% ethanol extract of P. amaryllifolius leaves cultivated in salinity soil contains compounds with biological activity that can be a good antioxidant source.