Live cell imaging of alkyne tagged small biomolecules by stimulated Raman scattering
Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne tagged small precursors.
Nature Methods