Anti Mullerian Hormone Is an Endocrine Marker of Ovarian Gonadotropin Responsive Follicles and Can Help to Predict Superovulatory Responses in the Cow 1
The major limitation to the development of embryo production in cattle is the strong between animal variability in ovulatory response to FSH induced superovulation, mainly due to differences in ovarian activity at the time of treatment. This study aimed to establish whether anti Mullerian hormone (AMH) was an endocrine marker of follicular populations in the cow, as in human, and a possible predictor of the ovarian response to superovulation. Anti Mullerian hormone concentrations in plasma varied 10 fold between cows before treatment and were found to be highly correlated with the numbers of 3to 7 mm antral follicles detected by ovarian ultrasonography before treatment (r 1/4 0.79, P 0.001) and the numbers of ovulations after treatment (r1/4 0.64, P 0.01) Between animal differences in AMH concentrations were found to be unchanged after a 3 mo delay (r 1/4 0.87, P 0.01) indicating that AMH endocrine levels were characteristic of each animal on a longterm period. The population of healthy 3to 7 mm follicles was the main target of superovulatory treatments, contained the highest AMH concentrations and AMH mRNA levels compared with larger follicles, and contributed importantly to AMH endocrine levels. In conclusion, AMH was found to be a reliable endocrine marker of the population of small antral gonadotropin responsive follicles in the cow. Moreover, AMH concentrations in the plasma of individuals were indicative of their ability to respond to superovulatory treatments. AMH, assisted reproductive technology, follicular development, granulosa cells, ovary