Preparation, characterization, and antibacterial activity of silver nanoparticle decorated graphene oxide nanocomposite.
In this work, we report a facile and green approach to prepare a uniform silver nanoparticles (AgNPs) decorated graphene oxide (GO) nanocomposite (GO Ag) The nanocomposite was fully characterized by transmission electron microscopy (TEM) Fourier transform infrared (FTIR) spectra, ultraviolet visible (UV vis) absorption spectra, and X ray photoelectron spectroscopy (XPS) which demonstrated that AgNPs with a diameter of approximately 22 nm were uniformly and compactly deposited on GO. To investigate the silver ion release behaviors, HEPES buffers with different pH (5.5, 7, and 8.5) were selected and the mechanism of release actions was discussed in detail. The cytotoxicity of GO Ag nanocomposite was also studied using HEK 293 cells. GO Ag nanocomposite displayed good cytocompatibility. Furthermore, the antibacterial properties of GO Ag nanocomposite were studied using Gram negative E. coli ATCC 25922 and Gram positive S. aureus ATCC 6538 by both the plate count method and disk diffusion method. The nanocomposite showed excellent antibacterial activity. These results demonstrated that GO Ag nanocomposite, as a kind of antibacterial material, had a great promise for application in a wide range of biomedical applications.
ACS applied materials interfaces