Population genetic diversity of Kappaphycus alvarezii cultivated in China
Random Amplified Polymorphic DNA(RAPD)markers were used to estimate the population genetic diversity in six cultivated populations of Kappaphycus alvarezii in Hainan Province,China.Six primers(S345, S352,S384,S1510,S2003,S2114)were selected from 30 primers and 43 reproducible and discernible loci were amplified.The amplified fragment sizes ranged from 300 bp to 3 kb.The mean percentage of polymorphic loci was 98.27%.By using software POPGENE 32,the percentages of polymorphic loci of six populations of K.alvarezii were 62.79% 97.67%;Nei's(1973)gene diversity indices(H)were from 0.1455+ 0.1809 to 0.2463+ 0.1797;Shannon's information indices(I)were from 0.229 1+ 0.256 6 to 0.380 8+ 0.242 5;And genetic distances were 0.015 1 0.135 0. It indicated that the genetic variations among populations were high,and the genetic diversity index of population S (Neicun,Sanya,Hainan)was the highest.MEGA4 software was used for UPGMA dendrogram analysis.The result showed that population S clustered alone,and LA and XC first superficially clustered together,then ordinally clustered with LD,L and NC.The six populations really belong to the same species of Kappaphycus alvarezii,although differeces in shape and color exist among them.