IMPACT OF GEOGRAPHICAL VARIATION ON RUTIN CONTENT FROM MIMOSA PUDICA L. USING HPTLC TECHNIQUE
Mimosa pudica L. (Mimosaceae) commonly known as touch me not is reported in treatment of menorrhagia, dysfunctional uterine bleeding, and urinary tract infections etc. It is reported for various pharmacological activities like antiulcer, anti inflammatory, anticancer activity etc, due to the presence of various reported biomarkers like gallic acid, mimosine, mimosinic acid, betasitosterol etc. Mimosa pudica is also a part of various traditional formulations like Samangadi Churna, Pushyanuga Churna etc. In order to standardize the extract, phytochemical and proximate analysis of Mimosa pudica (whole plant) has been carried out along with its chromatographic evaluation. HPTLC fingerprint has been developed as a quality assurance tool. Further, a HPTLC method has been developed and validated as per ICH guidelines to quantitate the content of rutin from plant samples collected from various geographical regions of India. Extraction efficiencies of solvents of varying polarity and effect of various extraction techniques, on plant samples in terms of marker content were also analyzed. The concentration of phytochemical marker varied in samples collected from different regions. Variation was also observed in the samples extracted using solvents of varying polarity and different extraction techniques. The data generated in the current study can be used as a quality control tool for the use of authentic sample of Mimosa pudica singularly and as a part of various medicinal formulations. The method developed for the estimation of Rutin can be applied to various biological matrices. The variation in content of rutin implies the effect of regional and climatic variation on marker content.