Optimizing SIC assay method for acetyl salicylic acid and rosuvastatin and adapting to HPLC with performance comparison
Acetyl salicylic acid (ASA) as the major drug consumed in the world, has been combined with other various drugs. In this regard, ASA rosuvastatin is the most recent combination. The current article reported the first two separation methods for the assay of that combination utilizing sequential injection chromatography (SIC) and high performance liquid chromatography (HPLC) The optimization process was conducted for SIC, and the optimum conditions were then adapted to HPLC. Both methods, SIC and HPLC, were validated and successfully applied to tablet formulation. In both methods, the analytes were chromatographed onto a short C18 monolithic column (4.6 x 25 mm) by a mobile phase composition of 10 mmol L 1 phosphate:acetonitrile: methanol (50:30:20, v/v/v at pH 3.0) The performances of both methods were compared. The remarkable advantages of the SIC method over HPLC method were the reduction in reagent consumption and instrumentation simplicity and cost effectiveness. The total volume of mobile phase con.