A novel "turn on" mitochondria targeting near infrared fluorescent probe for determination and bioimaging cellular hydrogen sulfide.
Hydrogen sulfide (H2S) has been regarded as an important gas transmitter playing vital role in cytoprotective processes and redox signaling. It is very meaningful to monitor and analyze it in biosystem for obtaining important physiological and pathological information. Despite numerous fluorescent probes for cellular H2S have been reported in past decades, only a few have capability to detect mitochondrial H2S with near infrared (NIR) emission. Therefore, a new mitochondria targeting NIR fluorescent probe (Mito NSH) for detection of cellular H2S was developed by introducing 2,4 dinitrophenyl ether into a novel dye (Mito NOH) A large "turn on" NIR fluorescence response was obtained due to thiolysis of ether to hydroxyl group when Mito NSH was treated with NaHS. Moreover, Mito NSH could quantitatively detect H2S at concentration ranging from 0 to 30 mM with a detection limit of 68.2 nM, and it exerts some superior optical properties, such as large stokes shift (107 nm) highly selectively mitochondria location, fast response and high selectivity to H2S. More impressively, it was successfully applied to imaging exogenous and endogenously generated H2S in living HeLa cells via confocal fluorescence microscopy.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy