Isolation and characterization of a heterologously expressed bacterial laccase from the anaerobe Geobacter metallireducens
Bioinformatics has revealed the presence of putative laccase genes in diverse bacteria, including extremophiles, autotrophs, and, interestingly, anaerobes. Integrity of laccase genes in anaerobes has been questioned, since laccases oxidize a variety of compounds using molecular oxygen as the electron acceptor. The genome of the anaerobe Geobacter metallireducens GS 15 contains five genes for laccase like multicopper oxidases. In order to show whether one of the predicted genes encodes a functional laccase, the protein encoded by GMET_RS10855 was heterologously expressed in Escherichia coli cells. The His6 tagged enzyme (named GeoLacc) was purified to a large extent in the apoprotein, inactive form: incubation with CuSO4 allowed a 43 fold increase of the specific activity yielding a metallo enzyme. The purified enzyme oxidized some of the typical laccase substrates, including 2,2' azino bis(3 ethylbenzothiazoline 6 sulphonic acid) (ABTS) syringaldazine, and 2,6 dimethoxyphenol (2,6 DMP) along with pyrogallol and K4[Fe(CN)6] Temperature optimum was 75 degC and pH optimum for ABTS and 2,6 DMP oxidation was 6.0. As observed for other laccases, the enzyme was inhibited by halide anions and was sensitive to increasing concentrations of dimethyl sulfoxide and Tween 80. Notably, GeoLacc possesses a very high affinity for dioxygen: a similar activity was measured performing the reaction at air saturated or microaerophilic conditions.
Applied Microbiology and Biotechnology