HIGH THROUGHPUT SCREENING METHODS IN THE DIRECTED EVOLUTION OF PROTEINS
Directed evolution is a new powerful strategy for engineering proteins.Many feasible methods for generating gene libraries are developed.Therefore developing the high throughput screening methods for desired mutants is the key to the success in directed evolution.In this article,we summarize the high throughput screening methods developed resent years such as FACS(Fluorescence activated cell sorting) and the display technology including phage display,cell surface display,ribosome display and mRNA peptide fusion.Their principles and application in screening for desired mutants from mutant libraries are introduced.We discuss the unresolved questions and promising prospect in the screening methods.