Solid State Hydrogen Deuterium Exchange Mass Spectrometry: Correlation of Deuterium Uptake and Long Term Stability of Lyophilized Monoclonal Antibody Formulations.
Solid state hydrogen deuterium exchange with mass spectrometric analysis (ssHDX MS) has been used to assess protein conformation and matrix interactions in lyophilized solids. ssHDX MS metrics have been previously correlated to the formation of aggregates of lyophilized myoglobin on storage. Here, ssHDX MS was applied to lyophilized monoclonal antibody (mAb) formulations and correlated to their long term stability. After exposing lyophilized samples to D2O(g) the amount of deuterium incorporated at various time points was determined by mass spectrometry for four different lyophilized mAb formulations. Hydrogen deuterium exchange data were then correlated with mAb aggregation and chemical degradation, which was obtained in stability studies of >2.5 years. Deuterium uptake on ssHDX MS of four lyophilized mAb formulations determined at the initial time point prior to storage in the dry state was directly and strongly correlated with the extent of aggregation and chemical degradation during storage. Other measures of physical and chemical properties of the solids were weakly or poorly correlated with stability. The data demonstrate, for the first time, that ssHDX MS results are highly correlated with the stability of lyophilized mAb formulations. The findings thus suggest that ssHDX MS can be used as an early read out of differences in long term stability between formulations helping to accelerate formulation screening and selection.
Molecular pharmaceutics