Ultrasensitive competitive method based electrochemiluminescence immunosensor for diethylstilbestrol detection based on Ru(bpy)32+ as luminophor encapsulated in metal organic frameworks UiO 67.
In this work, Ru(bpy)32+ encapsulated in metal organic frameworks (MOFs) UiO 67 (Ru(bpy)32+/UiO 67) as luminophor was easily prepared and firstly applied in constructing an electrochemiluminescence (ECL) immunosensor to efficiently estimate diethylstilbestrol (DES) The competitive method based ECL immunosensor platform was fabricated by amino silicon dioxide which possesses large surface area. The poriness of UiO 67 was splendid so that Ru(bpy)32+ could be easily encapsulated. Ru(bpy)32+/UiO 67 with excellent ECL luminescence signal existed large specific surface area for easily labeled with antibodies. DES competed with bovine serum albumin diethylstilbestrol (BSA DES) for binding to antibody specific sites in the constructed immunosensor. However DES was micromolecule, which was easier to bond to antibodies than BSA DES. The ECL signal was gradually decreases with the increase of the concentration of DES. Under optimal conditions, the proposed immunosensor exhibited a wide linear range from 0.01 pg mL 1 to 50 ng mL 1 with a low detetion limit of 3.27 fg mL 1 (S/N 3) The novel fabricated immunosensor with interference immunity and high stability may cause an attractive approach for the other targets determination.
Biosensors bioelectronics