Proteolysis Targeting Chimaeras Induce the Degradation of Epidermal Growth Factor Receptor in Lung Cancer
The PROTAC degraders synthesized and assessed in this project were designed as follows: To characterize the EGFR PROTACs, we used: HCC 827 and NCI H 3255 human lung carcinoma cell lines with mutant EGFR Western Blot to assess protein levels of EGFR and downstream targets upon drug treatments Cell Proliferation Assay to determine impact of PROTACs on lung cancer cell proliferation Liquid Chromatography Mass Spectrometry to assess the specificity of the EGFR PROTACs