EFFECT OF PROBIOTIC SUPPLEMENTED DIET ON GUT ASSOCIATED BACTERIAL FLORA OF LABEO
This study was focused to evaluate the effect of probiotic mixture diet on the growth performance, autochthonous gut bacterial population and extracellular enzymatic activities like amylase, protease and cellulase of Labeo rohita. The control diet contained fish meal (FM) and soybean meal (SBM) and the experimental diet contained FM and SBM supplemented with a Probiotic Mixture (PM) (LactoBacil plus: Swiss Garnier Life Sciences) at the rate of (CFU kg 1 =15x10 8 The feeding trial was conducted in flow through 90L circular fibre glass tanks for 90 days under laboratory condition. After completion of feeding trial the significant difference was found in SGR of fish fed with PM diet than the fish fed with FM and SBM containing control diet. The significant difference also found in PER of fish fed with PM meal which is good for fish health. After feeding trial, it was also found that total log viable counts increased in the hindgut (log viable count 7.64 g 1 intestinal tissue) of rohu fed with PM supplemented diet. Highest quantitative amylase activity recorded in the strain LBF6 (52.49+ 0.77 U) highest cellulase activity showed by the strain LBH6 (23.48+ 0.31 U) and highest protease activity exhibited by the strain LBH8 (2.29+ 0.05 U) isolated from PM fed fish gut.