Performance of MALDI TOF Mass Spectrometry to Determine the Sex of Mosquitoes and Identify Specific Colonies from French Polynesia.
Mosquitoes are the main arthropod vectors of infectious diseases in humans. The current methods for mosquito identification include morphological and molecular methods. Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI TOF MS) now routinely used for bacterial identification, has recently emerged in the field of entomology. The aim of this study was to use MALDI TOF MS to identify mosquito colonies from French Polynesia. Five hundred specimens from French Polynesia belonging to three species, Aedes aegypti, Aedes polynesiensis, and Culex quinquefasciatus, were included in the study. Testing the legs of these mosquitoes by MALDI TOF MS revealed a 100% correct identification of all specimens at the species level. The MALDI TOF MS profiles obtained allowed differentiation of male from female mosquitoes and the specific identification of female mosquito colonies of the same species but different geographic origin.
The American journal of tropical medicine and hygiene