Colorimetric determination of glucose in solution and via the use of a paper strip by exploiting the peroxidase and oxidase mimicking activity of bimetallic Cu Pd nanoparticles deposited on reduced graphene oxide, graphitic carbon nitride, or MoS2 nanosheets
AbstractThis work describes the preparation of bimetallic Cu Pd nanoparticles (NPs) on supports like reduced graphene oxide (rGO) graphitic carbon nitride (g C3N4) and MoS2 sheets with a size of <10 nm. rGO is found to be the best support for synthesizing Cu Pd NPs with controlled shape, size and oxidation state. The Cu Pd/rGO nanocomposite also demonstrated the best peroxidase and oxidase mimicking activity compared to Cu Pd/g C3N4 and Cu Pd/MoS2 nanocomposites. The peroxidase mimicking activity of Cu Pd/rGO was investigated in more detail, and a glucose oxidase (GOx) based glucose sensor was constructed that is based on the enzymatic formation of H2O2 and the Cu Pd NPs assisted oxidation of tetramethylbenzidine by H2O2 to give a blue green coloration with absorption maxima at 652 nm. The assay has a 0.29 mM detection limit and a detection range that extends from 0.2 to 50 mM. The method was applied to the determination of glucose in diluted serum samples, and results compared well to those acquired with a clinical analyzer. The method also was applied in a colorimetric paper based test stripe that can detect glucose within 10 min. Graphical abstractSchematic representation of a sensitive colorimetric glucose assay based on bimetallic Cu Pd nanoparticles supported on 2D nanosheets, and construction of a paper based device for rapid glucose detection.
Microchimica Acta