Presence of nano sized chitosan layered silicate composites protects against toxicity induced by lead ions.
Protecting cells from toxicosis even apoptosis induced by a variety of toxic heavy metals stimulus has drawn more and more attentions. This study was designed to elucidate whether chitosan organic rectorite (CS OREC) composites exhibited any protective effects on altered oxidative stress parameter in PC12 cells exposed to lead ions (Pb2+ The cells were exposed to Pb2+ either alone or in combination with CS OREC composites for designated time to evaluate the efficacy of the composites on Pb2+ induced toxicity. The MTT assay results showed that the cell viability of PC12 was remarkably decreased when exposed to Pb2+ but significantly retained after adding CS OREC composites compared to that of the control. The beneficial effect of CS OREC composites on cytotoxicity was related, at least in part, to its ability to protect against apoptosis in PC12 cells exposed to 50mM Pb2+ Their protective effect was also associated with the inhibitory effect on Pb2+ induced activation of Bax/Bcl 2, P 38, and caspase 3 pathways, while was independent on JNK pathway.
Carbohydrate polymers