SINTESIS SENYAWA ASAM O (4 NITROBENZOIL) 5 METOKSISALISILAT) DAN UJI AKTIVITAS ANALGESIK PADA MENCIT (Mus musculus)
Background: Salicylic acid is a drug used for analgesic therapy, but has a smaller effect than aspirin. Synthesis of O (4 nitrobenzoil) 5 methoxisalicylic acid was obtained to obtain drugs with low side effects and higher analgesic activity compared to aspirin. Objective: To produce O (4 nitrobenzoil) 5 methoxisalicylic compounds by reaction between 5 methoxisalicylic acid and 4 nitrobenzoyl chloride and to find out the analgesic activity in mice (Mus musculus) compared to aspirin compounds. Methods: Compound synthesis was carried out through the esterification of acyl halides, purified using TLC test with three types of eluents namely Acetone: Methanol (5: 5) Acetone: Ethanol (9: 1) Ethyl acetate: Methanol (3: 7) Then the structure was identified, and the analgesic activity was tested using the stretching method (Writing test) Results: O (4 nitrobenzoil) 5 methoxisalicylic acid formed in the form of crystalline, yellow leucorrhoea, odorless with a yield percentage of 13.00% and melting point 241 243@C. From the thin layer chromatography test obtained a single stain. The identification of a UV Vis spectrophotometer showed a wavelength of 208 nm, and 258 nm. The IR spectrophotometer shows wave numbers 3444.65 cm 1 OH) 2992.56 cm 1 (CH3) 1693.32 cm 1 (C O) 1541.46 cm 1 (C C) and 1280.39 cm 1 (COOC) In a 1H NMR spectrometer showing 10 atoms H. The pain barrier percentage obtained at doses of 25, 50, and 100 mg/kgBB respectively is 39.03 mg/kgBB, 45.33 mg/kgBB, and 55.69 mg/kg kgBB. ED50 O (4 nitrobenzoil) 5 methoxisalicylic compound is 65.92 mg/kgBB greater than aspirin compound 142.23 mg/kgBB. Conclusions and Suggestions: O (4 nitrobenzoil) 5 methoxisalicylic acid compounds were successfully synthesized with higher analgesic activity than aspirin. Further research is needed to develop alternative candidates for analgesic drugs.