Cationic solid lipid nanoparticles loaded by integrin b1 plasmid DNA attenuates IL 1b induced apoptosis of chondrocyte
Aging related inflammation is tightly linked with the development of osteoarthritis (OA) As the pro inflammatory cytokine, IL 1b has been associated with physical dysfunction and frailty. It is still elusive whether and how IL 1b blockade improves the outcome of OA. Here we develop a cationic solid lipid nanoparticles (SLNs) system that effectively mediate non viral delivery of plasmid DNA (pDNA) into cells. Compared with other DNA transfer technologies including lipofetamin 2000, SLNs pDNA system is less toxic and exerts identical effectiveness on DNA transfer. Loaded with integrin b1 overexpression pDNA, the SLNs pDNA mainly localized in cytoplasm and enforced expression of integrin b1 in rat chondrocytes. Moreover, upon exposure to IL 1b stimulation, SLNs pDNA treatment attenuates the apoptosis rat chondrocytes and augments tissue repair. Our data thus demonstrate that SLNs pDNA functions as a potential therapeutic nanomedicine in the treatment of osteoarthritis.
Aging