Microbial Diversity In Weaver Ant (Oecophylla) Gut
The aim to study the microbial ecology is to use metagenomics tools to clearly understand the complete genomes of uncultured microorganisms present in the environment. This is done to enhance applications in finding new or novel microbial enzymes, signal molecules as antibiotics and many more. Ant gut microbiome plays a very important part in hosts development, growth and their environmental habitat. The present research on the ant gut microbiome showstheir important role in exploiting many biotechnological applications using metagenomics tools. Therefore, 16S/18S/ITS rDNA serve as identifiers of biological species, and are important for microbial phylogeny and taxonomic identification. 16S/18S/ITS rDNA amplicon sequencing has become an important tool for the study of the composition of microbial communities in weaver ant gut, and the order and genus of bacteria found are Actinomycetales, Bifidobacteriales, Actinobacteria, Bacteriodales, Flavobacteriales, Caulobacterales, Methanobacteriales, Lactobacillales, Clostridiales, Bradyrhizobacterium, Agrobacterium etc .Hence, in this review, the complete microbialdiversity of ant gut is studied by following 16S 18S ITS rDNA amplicon sequencing procedure includes,dissection, culturing,genomic DNA extraction, quality control, rDNA variable region amplification, library construction, high throughput sequencing, data analysis and identification of microbiome is done to exploit their important functional role in the scientific research like the use in biotechnological applications. Index Terms Metagenomics,16s/18s/ITSrDNAidentifiers, DNA extraction, Nonculturable microbes, culturable microbes Microbiome, Weaver ant gut,