The lncRNA myocardial infarction associated transcript centric competing endogenous RNA network in non small cell lung cancer
Background The leading cause of death for cancer is lung cancer, of which the majority subtype is non small cell lung cancer (NSCLC) Recent studies have shown long non coding RNAs are transcribed and contribute to cancer. Previous study has shown that a few single nucleotide polymorphisms (SNPs) in myocardial infarction associated transcript (MIAT) were associated with some diseases or function as competing endogenous RNA (ceRNA) in some cancer. Patients and methods We performed bioinformatic methods for analyzing RNA seq and miRNA seq data of NSCLC from The Cancer Genome Atlas database. 1352 NSCLC patients and 1320 cancer free controls for genotyping, and dual luciferase reporter assay, real time PCR are performed in A549 and H1975 lung cancer cell lines. Results are analyzed by SPSS v16.0. Results In the present study, we focus on the role of over expression MIAT in NSCLC. We confirmed that rs1061451 T>C (allele odds ratio 0.22; P 0.01) was associated with NSCLC. Furthermore, we constructed MIAT centric ceRNA network, and three mRNAs (MYO1B, SGK1 and WNT9A) was identified as targets by MIAT via miR 133a 5p. Conclusion C containing genotypes of MIAT rs1061451 were protective factor of NSCLC, and MIAT, which may act as ceRNA via miR 133a 5p, modulated MYO1B, SGK1 and WNT9A expression level.
Cancer management and research