Aspergillus niger Enhance Bioactive Compounds Biosynthesis As Well As Expression of Functional Genes in Adventitious Roots of Glycyrrhiza uralensis Fisch
In the present study, the culture conditions for the accumulation of Glycyrrhiza uralensis adventitious root metabolites in balloon type bubble bioreactors (BTBBs) have been optimized. The results of the culture showed that the best culture conditions were a cone angle of 90deg bioreactor and 0.4 0.6 0.4 vvm aeration volume. Aspergillus niger can be used as a fungal elicitor to enhance the production of defense compounds in plants. With the addition of a fungal elicitor (derived from Aspergillus niger) the maximum accumulation of total flavonoids (16.12 mg g 1) and glycyrrhetinic acid (0.18 mg g 1) occurred at a dose of 400 mg L 1 of Aspergillus niger resulting in a 3.47 fold and 1.8 fold increase over control roots. However, the highest concentration of polysaccharide (106.06 mg g 1) was achieved with a mixture of elicitors (Aspergillus niger and salicylic acid) added to the medium, resulting in a 1.09 fold increase over Aspergillus niger treatment alone. Electrospray ionization tandem mass spectrometry (ESI MSn) analysis was performed, showing that seven compounds were present after treatment with the elicitors, including uralsaponin B, licorice saponin B2, liquiritin, and (3R) vestitol, only identified in the mixed elicitor treatment group. It has also been found that elicitors (Aspergillus niger and salicylic acid) significantly upregulated the expression of the cinnamate 4 hydroxylase (C4H) b amyrin synthase (b AS) squalene epoxidase (SE) and a cytochrome P450 monooxygenase (CYP72A154) genes, which are involved in the biosynthesis of bioactive compounds, and increased superoxide dismutase (SOD) catalase (CAT) and peroxidase (POD) activity.
Applied Biochemistry and Biotechnology