Polysomal Profiling is a technique that uses cycloheximide to arrest translation and a sucrose gradient to separate the resulting cell extract by centrifugation.[1] Ribosome-associated mRNAs migrate faster than free mRNAs and polysome associated mRNAs migrate faster than ribosome associated mRNAs. Several peaks corresponding to mRNA are revealed by the measurement of total protein across the gradient. The corresponding mRNA is associated with increasing numbers of ribosomes as polysomes.