In politics the theory of patriarchy was complicated by regencies led by Margaret Tudor and Mary of Guise and by the advent of a regnant queen in Mary, Queen of Scots from 1561. Concerns over this threat to male authority were exemplified by John Knox's The First Blast of the Trumpet Against the Monstruous Regiment of Women (1558), which advocated the deposition of all reigning queens. Most of the political nation took a pragmatic view of the situation, accepting Mary as queen, but the strains that this paradox created may have played a part in the later difficulties of the reign.[7] How exactly patriarchy worked in practice is difficult to discern.