Directed differentiation is a bioengineering methodology at the interface of stem cell biology, developmental biology and tissue engineering.[1] It is essentially harnessing the potential of stem cells by constraining their differentiation in vitro toward a specific cell type or tissue of interest.[2] Stem cells are by definition pluripotent, able to differentiate into several cell types such as neurons,[3] cardiomyocytes, hepatocytes, etc. Efficient directed differentiation requires a detailed understanding of the lineage and cell fate decision, often provided by developmental biology.[2][4]