Many clinical case trials occurred in the beginning of the 20th century. However, the very first clinical trial to have occurred dates back to the 19th century when Denninger conducted a trial prescribing children and pregnant women with calcium fluoride.[1] From this trial, it was recognised fluoride's significance on tooth tissue. From this point, many clinical trials were conducted [2] Following these studies, the recognition of the positive outcome on dental tissues became clear and projects in water fluoridation became of significant importance.