On 12 April 1648 Tollius was appointed professor of history and Greek at the University of Harderwijk, where he was also secretary of the College of Curators. In January 1654 he succeeded Georgius Hornius as professor of history, political science, and geography. Tollius died unexpectedly in Gouda, shortly after his marriage on 5 March to Margaretha van Kent, the daughter of the mayor of Gouda.