Authors of 3DISCO method used it first for studying regeneration in the central nervous system (CNS) of mouse, including counting of microglia, astrocytes and mapping trajectories of axons after injury.[3] 3DISCO was also used for mapping the development of mouse CNS.[2] Its modification iDISCO was used for functional studies of brain activity[4] or for mapping amyloid plaques, microglia, vasculature and other properties of brains in Alzheimer diseased patients and mouse models.[32] Modification uDISCO was then used for single cell mapping of neurons in whole unsectioned CNS of mouse.