Original sequence was CGAGAA coding for arginine & glutamine at 33 rd & 34 th position respectively, which became CG ATG AA creating a new start codon& new open reading frame. This new ORF is translated to 6-aminohexanoic acid hydrolase, a 392 amino acid length protein compared to 427 amino acids in the original protein.[5] Ohno suggested that many unique new genes have evolved this way.