PykF is allosterically regulated by fructose 1,6-bisphosphate (FBP) which reflects the central position of PykF in cellular metabolism.[4] PykF transcription in E. coli is regulated by the global transcriptional regulator, Cra (FruR).[5][6][7] PfkB was shown to be inhibited by MgATP at low concentrations of Fru-6P, and this regulation is important for gluconeogenesis.[8]