It is possible that proteins with low stoichiometry of phosphorylation, in very low abundance, or phosphorylated as a target for rapid degradation will be lost.[10] Bioinformatics analyses of low-throughput phosphorylation data together with high-throughput phosphoproteomics data (based mostly on MS/MS) estimate that current high-throughput protocols, after several repetitions are capable of capturing 70% to 95% of total phosphoproteins, but only 40% to 60% of total phosphorylation sites.[2]