Neothramycin shows weak antimicrobial activity compared to other pyrrolo(1,4)benzodiazepines. It also shows lower toxicity in mice. Neothramycin has been shown to exhibit activity against Yoshida sarcoma in rats, leukemia P388, sarcoma 180, Ehrlich Ascites carcinoma, Walker carcinosarcoma-256, and light activity against leukemia L-1210 in mice.[6] These activities against cancer are typical to the activities seen in the other pyrrolo(1,4)benzodiazepine compounds.