Ine continued her studies in Nagasaki under Abe Roan.[lower-alpha 8][10] In 1854 she left Tada with her mother and went with Ninomiya Keisaku's nephew Mise Shūzō [ja] to study under Keisaku in Uwajima, whose lord, the daimyō Date Munenari, enthusiastically promoted Western learning.[11] After he suffered a stroke in 1856, Keisaku returned to Nagasaki with Ine and Shūzō.[12]