The flotilla operated mostly various marks of the Type VII U-boat and it concentrated its efforts mainly in the Mediterranean Sea, against convoys. In August 1943, the flotilla moved to Toulon, but did also have u-boats in Marseille and Salamis. The flotilla was disbanded in September 1944, when U-407 was sunk on September 19 and the two last flotilla boats U-565 and U-596 were scuttled in Salamis.