Tm calculations in OligoAnalyzer – Integrated DNA Technologies DNA thermodynamics calculations – Tm, melting profile, mismatches, free energy calculations Tm calculation – by bioPHP.org. https://web.archive.org/web/20080516194508/http://www.promega.com/biomath/calc11.htm#disc Invitrogen Tm calculation AnnHyb Open Source software for Tm calculation using the Nearest-neighbour method Sigma-aldrich technical notes Primer3 calculation "Discovery of the Hybrid Helix and the First DNA-RNA Hybridization" by Alexander Rich uMelt: Melting Curve Prediction Tm Tool Nearest Neighbor Database: Provides a description of RNA-RNA interaction nearest neighbor parameters and examples of their use.