Gloucester ran 15 boarding homes in New York and they lived in Brooklyn from 1855. Her husband founded the Siloam Presbyterian Church[3] and she helped to pay for the building of the church.[3] They hosted abolitionist John Brown and she contributed to his causes.[2] She purchased the Hamilton Club and turned it into an upscale boarding house called the Remsen House.[3] She hosted Fredrick Douglass, John Brown and many others at the house and held meetings for the Freedman's Friend Society, Ladies National Union Fair and Union Soldier Association.[4]