This and the following expeditions of Cristóvão Jacques to the River Plate and into the Parana River in 1521; and of Martim Afonso de Sousa and his brother Pero Lopes de Sousa, in 1530-1532, from the Amazon river, to Lagoa dos Patos and to the rivers Plate and Paraná, reinforced and demonstrated Portuguese interest in the River Plate.