Difflugia is the largest genus of Arcellinida, one of several groups of Tubulinea within the eukaryote supergroup Amoebozoa.[1] Arcellinida species produce shells or tests from mineral particles or biogeonic elements (e.g. diatom frustules) and are thus commonly referred to as testate amoebae or shelled amoebae. Difflugia are particularly common in marshes and other freshwater habitats.