Doctrinal disputes arose between Villegaignon and the Calvinists, especially in relation to the Eucharist, and in October 1557 the Calvinists were banished from Coligny island as a result.[1] They settled among the Tupinamba until January 1558, when some of them managed to return to France by ship together with Jean de Léry, and five others chose to return to Coligny island where three of them were drowned by Villegaignon for refusing to recant.[3]