Ho is the eldest of four children of retired businessman Ho Eng Hong and Chan Chiew Ping.[24][25] She has two brothers and a sister.[26] Her sister Ho Peng is the chairperson of the Singapore Examinations and Assessment Board while younger brother Ho Sing is executive director of Starhill Global REIT.[27] She met her husband, Lee Hsien Loong, the current Prime Minister of Singapore and eldest son of former Singapore Prime Minister Lee Kuan Yew, while starting out in her career at the Ministry of Defence.[28] They married on 17 December 1985 and have two sons, Hongyi and Haoyi.