Authors of iDISCO (stands for “immunolabeling-enabled imaging of solvent-cleared organs“) included pretreatment of sample with methanol, hydrogen peroxide, detergents and dimethyl sulfoxide (DMSO) together with antibody labeling before clearing. This preprocess overcome two drawbacks of antibody labeling of large samples. First lowering the autofluorescence of samples and enhance signal-to-noise ratio, and second make tissue more penetrable for antibodies.