Pasteur also experimented with the mechanisms of brewer's yeast in the absence of organic nitrogen.[1] By adding pure brewer's yeast to a solution of cane sugar, ammonium salt, and yeast ash, Pasteur was able to observe the alcoholic fermentation process with all of its usual byproducts: glycerin, succinic acid, and small amounts of cellulose and fatty matters.[6] However, if any of the ingredients were removed from the solution, no fermentation would occur.