The tricolored bat's natural predators include the northern leopard frog, birds of prey, raccoons, snakes, skunks, and prairie voles.[1][2] Feral cats will also catch and kill them as they leave their hibernacula (cave or mine used for hibernation).[3] There are also records of it being attacked by the hoary bat.[12][21] The tricolored bat is the host to several species of endoparasites (internal parasites) and ectoparasites.