The method was developed by team around Ali Ertürk and Hans-Ulrich Dodt from Max-Planck-Institute in Munich[1][2] primarily for clearing and imaging unsectioned mouse brain and spinal cord. Later on the method or its modifications were successfully used in many fields of biological research to image and investigate whole body of mouse,[3] structure and function of mouse brain,[1] stem cells,[4][5] tumor tissues,[6][7][8] developmental processes[9][10] or whole human embryos.[11]