RNA selection and depletion methods:[1] Strategy Type of RNA Ribosomal RNA content Unprocessed RNA content Genomic DNA content Isolation method Total RNA All High High High None PolyA selection Coding Low Low Low Hybridization with poly(dT) oligomers rRNA depletion Coding, noncoding Low High High Removal of oligomers complementary to rRNA RNA capture Targeted Low Moderate Low Hybridization with probes complementary to desired transcripts