In the simplest cases, the samples are combined into a group of a single color and the data is analyzed as described previously. However, by maintaining separate colors for each sample set, additional information on how the bubbles were formed, whether by error or by repeats, presents itself.[1] In 1997, the Department of Technology Development at Genzyme Genetics in Framingham, Massachusetts developed a new approach that provided a breakthrough in dealing with bubbles using the multiplex allele-specific diagnostic assay (MASDA). This program combines forward dot-blot, complex simultaneous probe hybridization and direct mutation detection to help solve the dual problem of multiple sample analysis.[6]