In 1884 Robert Warrington Jr. married Helen Louisa (1855–1898), third daughter of George H. Makins, M.R.C.S., chief assayer to the Bank of England.[1] Robert Warington Jr. and his first wife had five daughters: Elizabeth (Betty) born in 1888; Margaret born in 1890; Dorothy, born in 1892; and twins, Katherine and Helen (Kitty and Nell) born in 1897.[7] In 1902 he married Rosa Jane, daughter of Frederick Robert Spackman, M.D., of Harpenden.[1] Katherine Warington (1897–1993) became a famous botanist. Robert Warington Jr is buried with many of his family in a family plot in St Nicholas Churchyard, Harpenden, England.