RNA-Seq was first developed in mid 2000s with the advent of next-generation sequencing technology.[122] The first manuscripts that used RNA-Seq even without using the term includes those of prostate cancer cell lines[123] (dated 2006), Medicago truncatula[124] (2006), maize[125] (2007), and Arabidopsis thaliana[126] (2007), while the term "RNA-Seq" itself was first mentioned in 2008.[127] The number of manuscripts referring to RNA-Seq in the title or abstract (Figure, blue line) is continuously increasing with 6754 manuscripts published in 2018 (link to PubMed search).