Eckerson completed her bachelor's degree in 1905 and stayed at Smith College for her master's degree which she completed in 1907. During her time at Smith College Eckerson taught botany and plant microchemistry, working as a demonstrator and assistant in the botany department between 1905 and 1909. Travelling to the University of Chicago in 1909, Eckerson was awarded her PhD in 1911.[1]