Bolotov was found dead on 27 January 2017 in his own home in Moscow, Russia. The causes of his death are currently being investigated. The preliminary results of clinical tests showed an acute heart failure as reason of death.[10][11][12][13][14] The poisoning later was suspected.[15] Though more detailed report of the local police office claimed that there was no obvious signs of the acute heart failure and only a small atherosclerotic plaques was identified instead.[16] It is known that before death he was complaining to his wife about his health deterioration which happened right after drinking a cup of coffee on the business meeting in company with two men he allegedly knew.[17] It became later known that Bolotov met with ex-speaker of the People's council of the LNR Alexey Karyakin and Valery Alexadnrovich as Valery had said and added also that the meeting was appointed by request of Bolotov himself.[18] Bolotov's corpse was later tested for the presence of the poisoning drugs in his body at the request of his wife, but as of 2018 the results are unknown.[19]