Loveless hypothesized, however, that the allergens were concentrated in the insect venom rather than the whole body, and thus that injections of venom-sac extract would be a more effective therapy.[1] Since pure venom was unavailable, she collected and anesthetized insect samples herself before removing their venom sacs to prepare for injecting into her patients; by 1964, she had dissected some 30,000 insects, and reported that she had become so proficient at the process that she could dissect "a bug a minute".[1] From 1953 to 1956, she injected patients at her allergy clinic with increasing doses of venom and monitored their reactions to subsequent bee and wasp stings.[1] Receiving booster doses each year, these patients developed immunity to the insect venom and were no longer susceptible to anaphylaxis.[2]