Mary's husband, Lord Darnley, is said to have been jealous of their friendship, because of rumours that he had impregnated Mary, and joined in a conspiracy of Protestant nobles, led by Patrick Ruthven, 3rd Lord Ruthven, to murder him. The murder was the catalyst for the downfall of Darnley, and it had serious consequences for Mary's subsequent reign. Mary was having dinner with Rizzio and a few ladies-in-waiting when Darnley joined them, accused his wife of adultery and then had a group of knaves murder Rizzio[3], who was hiding behind Mary.