OpenCFU is written in C++ and uses OpenCV for image analysis.[8] NICE is a program written in MATLAB that provides an easy way to count colonies from images.[9][10] ImageJ and CellProfiler: Some ImageJ macros[11] and plugins and some CellProfiler pipelines[12] can be used to count colonies. This often requires the user to change the code in order to achieve an efficient work-flow, but can prove useful and flexible. One main issue is the absence of specific GUI which can make the interaction with the processing algorithms tedious.