Passage: The planned referendum was included in the Queen's Speech on 27 May 2015. It was suggested at the time that Cameron was planning to hold the referendum in October 2016, but the European Union Referendum Act 2015, which authorised it, went before the House of Commons the following day, just three weeks after the election. On the bill's second reading on 9 June, members of the House of Commons voted by 544 to 53 in favour, endorsing the principle of holding a referendum, with only the Scottish National Party voting against. In contrast to the Labour Party's position prior to the 2015 general election under Miliband, acting Labour leader Harriet Harman committed her party to supporting plans for an EU referendum by 2017.To enable the referendum to take place, the European Union Referendum Act was passed by the Parliament of the United Kingdom. It extended to include and take legislative effect in Gibraltar, and received royal assent on 17 December 2015. The Act was, in turn, confirmed, enacted and implemented in Gibraltar by the European Union (Referendum) Act 2016 (Gibraltar), which was passed by the Gibraltar Parliament and entered into law upon receiving the assent of the Governor of Gibraltar on 28 January 2016.

Question: How many people opposed the referendum?
53