Article: Open the vaccine vial or bottle and dilute it by using a syringe with 3 ml of diluent. The syringe and diluent should come with the vaccine. Make sure that the temperature of the diluent is 2° to 8° C (36° to 45° F).  To make sure that the diluent is always cold, always carry it in an ice box filled with ice. If you are going to vaccinate a lot of birds, you can divide the diluted vaccine into 2-3 clean bottles and keep them on ice. That way, the vaccine will stay at the right temperature. Gently shake the vaccine vial several times before attaching the dropper. Then, attach the eyedropper, which should have been provided with the vaccine and diluent. You should be able to attach the dropper by pulling it over the lip of the vaccine vial or by twisting it on. Gently grab the bird’s head and twist it slightly so that its eye is facing you. Position the dropper carefully over the eye and slowly squeeze out a drop of the vaccine. Then, wait a moment to ensure that the vaccine is absorbed by the eye instead of pooling and leaking out. If the drop is fully absorbed, the vaccine was successful.  If the drop was not fully absorbed, a new drop should be administered. It may be helpful to have an assistant hold the chicken in place while you administer the vaccine.
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Dilute the vaccine solution. Attach the eyedropper to the vaccine vial. Drop at least 0.03ml of the vaccine into the chicken’s eye.