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Mark the eggs with a pencil. Separate the eggs. Choose an incubation medium that will retain water and remain sterile. Keep the medium moist. Make places in the medium for the eggs with your finger. Move the eggs into the container very carefully. Place the container in the incubator.

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Once you uncover the eggs, you should not turn them. Lizard eggs begin developing almost immediately and attach to the side of the egg. Use a pencil to gently mark the side of the egg facing you when you found it. This helps you remember the top of the egg so you don't damage the lizard inside. Moving them or rolling them to the other side can damage the embryo and kill the developing lizard. Some lizards will lay their eggs in a pile, which makes them stick together. If you find them early enough, you should carefully separate the eggs, trying not to damage the fragile shells. If they are stuck together, don't force them apart. Separating the eggs help protect them. If one egg dies, mold or fungi from it can infect the healthy egg. The medium, or substance, that you place the eggs on inside the incubation tank is important. It should be able to retain water to help keep the incubator humid. It should be sterile or close to it, and it shouldn't promote growth of mold or fungi.  Perlite and vermiculite are common mediums that eggs can successfully incubate and hatch on. These mediums are very similar, and the choice is usually based on preference. You can find these substances at nurseries or home improvement stores with garden centers. Incubation medium goes on the bottom of the container you're placing your eggs in. Place about one to two inches (25 to 50 mm) of medium at the bottom of the container. It is important to keep the incubation medium moist while waiting for the eggs to hatch. Add water until the medium is barely clumping together. It should not be so wet that water drips out of it when it is squeezed. Keep the medium at this moisture level until the eggs hatch. Before you move the egg, make an indentation with your finger in the incubation medium where you want to put the egg. This gives the egg a secure place to lay so it won't roll around and hurt the embryo. This space also allows you to bury the egg slightly. The egg should be about half covered with the medium. Place multiple eggs about a fingers width apart from each other. Place the indentations in a line. When you are ready to move the eggs, use great care. First, make sure your hands are clean. Don't turn or roll the eggs around when moving them. Keep the top of the egg where you made a pencil mark facing up. Place them in the medium inside the container.  Don't let the eggs roll around when you move them. If the eggs are stuck on a branch, remove the branch and place it in the incubator.  Do not try to remove the eggs from the branch because they will break. Cut the branch as small as possible without disturbing the eggs and try to find a container big enough for the branch too. Close the lid on the container containing the eggs. Then, place the container in the incubator. Write down the date of when the eggs were laid and placed in the incubator, calculate when they should hatch, and mark it on your calendar.