So now notice we have this DNA molecule where one of the strands is this DNA template strand and the other strand is the newly synthesized green strand that contains this, this slightly modified codon sequence. Now, if we take this plasmid and repeat step one and two, so essentially we heated, we separated, then we basically isolate that green DNA molecule that was synthesized in step two. And now we add that DNA polymerase as well as the four types of deoxy nucleuside triphosphates. Now, the DNA polymerase will use this green strand that contains that point mutation to basically synthesize the complementary strand to the green strand. And so after step three, we essentially produce that plasmid molecule, the double stranded DNA molecule that will contain that point mutation. And now we can use this modify double stranded DNA molecule to basically synthesize that protein in which we replace the glutamate with the aspartape molecule.