Now, in that column, we basically have these bees. Now, we also have a beaker that contains a crude mixture of proteins. So we have three proteins, and we only want to isolate one of those three proteins. Now, what we know about these three proteins is one of those proteins binds to a specific molecule, let's say a glucose molecule, and the other two enzymes, proteins, do not bind to enzyme molecules. So we have a green molecule, which is enzyme number one, that has a high affinity for glucose. So it basically has an active side that can accommodate a glucose molecule.