So remember, the plasmid is this circular, double stranded DNA molecule. And let's suppose we want to delete, we want to remove this entire chunk of DNA shown by purple. So to remove this, all we really have to do is take those specific restriction enzymes that cleave at this position. And this position, we mix it with this plasmid molecule that basically cleaves, removes this unwanted section, and then we can essentially purify and remove that unwanted section by some type of process. For example, gel electrophoresis. And then we can add a DNA ligase molecule which will use ATP to basically create that phosphodiaste bond between this end and this end.