Well, the binding process differs for each one of these amino acids. And that's because they have different charge values and they have different types of side chain groups. And what that means is when we actually wash our column down with some type of material, when we wash them down, they will basically detach depending on what the concentration, what the PH, and what the volume is of that particular buffer. So we can use a buffer, for example, sodium side trait of increasing PH to basically wash down our column. And as we wash down the column, these amino acids will begin to elude at different rates. So the identity of the amino acid can be determined based on the volume of the buffer and the PH of the buffer that is needed to actually elude that amino acid.