Now, let's move on to equation seven. So by the same analogy, we see that the total enzyme inside our mixture is equal to the enzyme that is not bound to the substrate plus the enzyme that is bound to the substrate. Now, the same thing essentially what we assumed just a moment ago, we cannot assume for the enzyme case. And that's because the concentration of the enzyme is usually much, much smaller than the concentration of the substrate. For instance, we can have a million of the substrate molecules, but only 100 of the enzyme molecules. And so what that means is we can no longer make the assumption that this is much smaller than this.