So if our cells are to function correctly, the hemoglobin must be able to bring enough oxygen and release enough oxygen in the tissues of our body. And previously we spoke about one allosteric effector molecule, namely two, three BPG that binds into the center pocket in that deoxy hemoglobin, stabilizing the T state of the deoxy hemoglobin and decreasing the affinity of hemoglobin for oxygen. And this is precisely what allows that hemoglobin to basically release enough oxygen to the tissues of our body. Now, as it turns out, two, three BPG is not the only allosteric effect. Therefore hemoglobin, there are two other molecules found inside our body, inside the red blood cells that can bind onto a special region of the hemoglobin other than the oxygen binding side and decreased affinity of hemoglobin for oxygen, thereby shifting the oxygen binding curve to the right side. And these two molecules are hydrogen ions and carbon dioxide.