So here we have the DNA template that we're going to use to basically pair up the correct complementary basis. And this template is needed to actually form that polynucleotide chain correctly. So this is the DNA template, this is the three end and this is the five end. Now this is the primer. And the primer basically contains the sequence of nucleotides. And on the final nucleotide, we have the sugar with a base that contains this free three prime hydroxyl group.