And how did we discover this important fact about proteins in biochemistry? Well, basically in 1950s, an American chemist by the name of Christian Anthony conducted a series of important experiments that demonstrated that all the information that we need to basically form that threedimensional structure of the polypeptide is found is stored in the specific sequence of amino acids within that polypeptide. And later experiments confirm the fact that the primary structure determines that confirmation of that protein. Now, before we actually examine what these experiments were, let's take a look at some important molecules and reagents that he used in his experiment. So he used two important denaturing agents. Denaturing agents are these molecules that basically break down the structure of our protein.