And this position, we mix it with this plasmid molecule that basically cleaves, removes this unwanted section, and then we can essentially purify and remove that unwanted section by some type of process. For example, gel electrophoresis. And then we can add a DNA ligase molecule which will use ATP to basically create that phosphodiaste bond between this end and this end. So at the end, we produce this new synthesized plasmid in which we removed, we deleted a large chunk of that DNA molecule, as shown in this diagram. So we essentially removed this purple section from that plasmid. And now we can basically take the plasmid, place it into our bacterial cell, and that bacterial cell can synthesize the gene that the plasmid actually codes for.