Now, we are not yet done. Now, what we basically want to do is we want to assume this same condition that we assumed previously. So I said that under normal physiological conditions, when the reactions take place inside our cells, the concentration of the substrate is much smaller than the concentration than the Km value. So this is around where the concentration inside our cells is usually at. So basically, equation three, this equation here, as we'll see in just a moment, can be used to give us insight into how enzymes operate under typical physiological conditions. That is, when the concentration of the substrate is much, much smaller than our Km value.