We get that the concentration of the enzyme that is not bound to anything is equal to the total enzyme concentration minus the enzyme concentration that is bound to our substrate. And now we take equation five and replace this quantity with this here. And so we get the following result. Now we take this equation, we multiply this out and we solve for the enzyme substrate complex concentration because now we have this term appearing on the left side and this term appearing on the right side. So we want to solve for this enzyme substrate concentration and we get the following result. So the enzyme substrate concentration is equal to the total enzyme found inside the midstrip multiplied by the substrate concentration divided by Km.