And once that takes place, this structure is basically dissociated, it goes on and binds onto the phospholipac, which stimulates this enzyme to cleave the Pip two. And once the pip two is cleaved into two structures, the Dag and the IP three, these are the secondary messenger molecules. IP three goes on and binds onto this calcium channel, opens the calcium channel up and allows the movement of these calcium ions from the lumen of the Er into the cytoplasm. Then the calcium can either go on and bind to calmodulen, which forms a complex that can initiate many different types of processes, or it goes on and binds to protein kinase C. And in this case, with the help of Dag. It initiates protein kinase C, which goes on and basically carries out different types of cell processes and activates other enzymes that initiate cellular processes. Now, one last thing I want to mention is the following one actually allows the cell to establish an electrochemical gradient in which we have many calcium ions in the Er lumen and very few calcium ions in the cytoplasm.