Now, biotin is basically a water soluble B vitamin. Specifically, it's the B seven vitamin while our Thiamine pyrophosphate is also a B vitamin derivative. Now, our coenzymes can be subdivided into these two groups but what exactly is the major difference between these two groups? As discussed earlier? So we have the prosthetic group which basically bind tightly to the enzyme. So these coenzymes bind tightly, usually via Covalent bonds to our enzyme and remain tightly bound during the reaction.