So once we obtain the source of a mixture of proteins and inside that mixture protein, we have that protein that we want to study that will act as a source. And once we have the source, we now can use a variety of different types of specific purification processes. So proteins can be generally proteins can be generally separated based on four important different types of properties. So based on the size of the protein, based on the solubility of the protein, how well dissolves in a certain type of solvent, based on the charge found on that protein, and also based on the ability of that specific protein to bind to some specific type of biological molecule. For example, certain types of proteins are able to bind to specific type of DNA sequences and DNA molecules, while other proteins cannot bind to those DNA molecules. And using that property, we can separate them because of that ability to bind.