And positive super coils basically destabilize our molecule by increasing its energy. So to stabilize the unwound DNA molecule, a protein known as DNA gyrates has to bind to our molecule and introduces negative super coils which decreases the number of positive super coils and therefore stabilizes by decreasing the amount of stress that our molecule experiences. Now, once the helicase unwinds our DNA from this picture, we see that we expose these individual complementary single stranded DNA molecules. And once we expose these two molecules another type of enzyme known as primates which is basically an RNA polymerase binds to each one of these two individual and exposed single stranded DNA molecules and it adds a set or a series of nucleotides that together are known as RNA primer. So primase adds a series of nucleotides onto these two exposed single stranded DNA molecules and these are known as RNA primer. An RNA primer basically acts as a signal and signals for another protein known as DNA polymerase to actually bind to that primer region and initiate DNA replication.