So we see that the net charge on the protein is negative two. And what that means is our estimate, our guess, was incorrect. At a PH of seven, this protein will have a charge of negative two. Now, do we go up or do we go down? So basically, because we have a charge of negative two, that means we want to decrease the amount of negative charge we have on our protein. Now, if we go higher, if we go above eight, we will remove this positive charge.