So once again, isozymes are these different forms of the same type of enzyme and these isozymes come from different genes. And what that means is they're going to have different amino acid sequences and different threedimensional structures, but they will catalyze the same type of biochemical reaction. So we see that ISMES arise from different genes, they have different sequences of amino acids and what that means is they will generally have different biochemical properties. And this is exactly what allows us to actually separate or purify mixtures of isozymes. Now, not only that, but Icimes also generally have different enzyme kinetics. So they differ in the Km value, the mechanism constant in a turnover number Kcat, and they also differ in things like the V max, the maximum velocity at which the enzyme operates.