So we have enzyme number one in this, and we have a bunch of enzymes number one throughout our beads, throughout the column. Now, enzyme two and three don't bind to any of the bees, and so they simply continue moving all the way to the bottom. So we see that the mixture of protein two and three will essentially be mixed together because none of them actually bind, none of them are attracted to those glucose molecules bound to our beets. And so in step four, we essentially turn the knob and we open up our hole. And so that allows the movement of these two proteins that we did not want to separate in the first place. And we basically place them in this beaker.