Okay, so what exactly is a three to five or three prime to five prime phosphol diastolinkage? So let's take a look at the following subsection of our nucleic acid. Now notice because this sugar doesn't have a hydroxyl group on that second carbon. So this is carbon number one, carbon number two, three, four and five. Because there is no oxygen here, that means this must be a DNA nucleic acid. So we have a single strand of DNA and notice how these sugars are actually connected to one another.