And if we take a protein that contains a net negative charge, it will begin to move away from this end and towards this positively charged end. Now, the proteins will continue moving along our gel until they reach the PH value at which the overall charge is zero. And when the overall charge is zero, because there is no net charge, those proteins will no longer move along that electric field. So when the protein reaches its specific isoelectric point, the pi value, it will be, it will stop moving within that gel. And so if we, for example, have a mixture of three proteins that each have their own unique ISO electric point value, and we place them into our mixture, they will separate until they will separate, and they will stop moving when they reach their pi value. So for protein one, the pi value is acidic, or relatively acidic.