So we essentially have these values that we can find in a textbook and then we can actually determine what the values that we use are, and then we can compare them to those textbook values and see exactly what amino acid we are dealing with. So, to see what we mean, let's suppose we use a buffer at a relatively low PH, and we use a very small amount of that volume before we see that one of those amino acids actually eludes. And so if we know if we calculate how much volume we used and what the PH of that is, and we compare to our textbook value, we'll see that the first amino acid was this green amino acid, which is aspartate. So d. Now we continue our process, we continue pouring and we increase the amount of volume and we might change our PH. And then once we see that a second amino acid loops, then we write down how much volume we use and what the PH of that buffer was. We compare it to our textbook value and we see that it is this quantity here.