And this entire process is catalyzed by single enzyme. Now, once we form carbon well phosphate, this then leaves the active side of CPS and it moves into the active side of aspartade, transcarbolase a different enzyme. What this enzyme does is it takes an amino acid aspartate it attaches it onto this carbon here and it kicks off this orthophosphate group to form carbon oil aspartate. Now, in the next two steps, what we ultimately want to achieve is we want to build that six membered primidine ring, as shown here, that we're ultimately going to use to attach onto a sugar molecule to form that perimedine nucleotide. So in this step, we basically have a dehydration step. We essentially release a water molecule.