Now, this section is basically the origin of replication. This is where replication essentially initiates. So remember, because circular DNA, unlike linear DNA, do not have a beginning and an end, we have to have the origin of replication position for the DNA polymerase to actually know where to begin the process of replication. That's why we have the origin of replication. So the PBR three, two, two plasmid contains genes that give it resistance to antibiotics. Ampicillin, the green one and tetracycline, that blue one.