Now, in the presence of water, which we have plenty in a cytoplasm, remember, all these steps basically take place in a cytoplasm except step number one and step number ten. And so the water is used to hydrolyze this ester bond via the activity of fructose one six bisphosphatase. And remember, it's a phosphatase, because phosphatases take off phosphoryl groups while kinase is actually put on those phosphoryl groups. And we basically break the bond and we form this fructose six phosphate and we release that inorganic phosphate, the orthophosphate as shown here. Now, this enzyme is an allosteric enzyme. And what that means is it contains allosteric regulatory sites.