And so because we had you read in the mixture improper incorrect disulfide bonds were actually formed. So when the beta mercapto ethanol was removed, first an inactive enzyme was formed. And this is because the improper disulfide bonds were formed because those non covalent reactions could not basically dictate the formation of those proper disulfide pairings. Now let's move on to experiment number three. So in experiment number three, what he saw is if he took this scrambled enzyme and he added a tiny amount of beter mccaptoethanol. So basically we have a beaker that contains our scrambled enzyme and we don't have any type of agent inside that beaker.