And finally, let's carry out the final one. So in the final step, in step E, we take our extracted mixture of proteins and we expose it to affinity Chromatography. And in affinity Chromatography, we separate our proteins based on their specific ability to bind to these special molecules. And this usually creates a very high specific activity value because as we know, enzymes only bind to specific substrate to specific molecules. So let's see if that's true by taking the extracted mixture and placing it into our last well. So what we produce is a band distribution that only contains a single band.