Now let's suppose we take the DNA molecule, we expose it to our restriction enzymes and we create these many DNA fragments. And let's say that one of these fragments contains a specific gene that we want to study. The question is how do we identify that specific fragment that is carrying the gene and how do we isolate and separate that fragment to basically study it further? Well, we have to carry out a process known as southern Blotting. In southern Blotting we essentially use gel electrophoresis as we'll see in just a moment to basically identify and isolate a specific DNA fragment of interest. So let's begin with step number one.