And on both ends we basically contain those green sections that we want to remove. So in the next step, we add a molecule we call an exonuclease. And what an exonuclease does is it essentially removes these tiny portions at the end. And once the exonuclease removes these two portions, we can remove that exonuclease, remove these two tiny portions and then add DNA ligase to basically create that phosphodiasta bond between these two ends that now don't have these green sections. And so now we contain this new gene sequence, this new gene that now contains this new DNA sequence. And now we can add it into our bacterial cells and once again it can synthesize the protein that the plasmid actually codes for.