And so we have a PKA of 10.9, which is above seven. And so what that means this will have a neutral charge, just like in the other two cases. Let's move on to the third amino acid. So this one here, Lysine, PKA of 10.8, which is above seven, that means it will have a charge of positive one on that nitrogen. We move on to the next amino acid in line, which is glutamate has a PK of 4.1, which is below our neutral PH of seven. So this will bear a negative charge on the oxygens here.