And in the process, our electron transport chain establishes an electrochemical gradient between the intermembrane space of the mitochondria and the mitochondrial matrix. And one of the proteins of the electron transport chain known as ATP synthase utilizes the electrochemical gradient to synthesize our ATP molecules. Now we see that a single NADH molecule produced in the mitochondrial nature creates three ATP molecules. So that means our NADH that is formed in Pyruvate decarboxylation and the citric acid cycle which both take place in the mitochondrial matrix each produce three ATP molecules while the NADH that is formed in the cytosol of our cell via glycolysis produces only two ATP molecules. And that's because we actually have to use a single ATP molecule per one NADH to transport that NADH from the cytoplasm to the mitochondrial matrix. Now, we also see that a single fadh two that is formed in the citric acid cycle produces two ATP molecules.