So this section that is known as the pellet and the rest ends up at the surface. So higher up this entire section that is known as the supernatural. Now in the first centrifugation process, our acceleration is 1000 g's, where g is simply the gravitational pull due to the earth 9.8 meters/second squared. So 1000 G's basically means the molecules and things inside our test tube experience a gravitational or an acceleration that is 1000 times as high as the gravitational acceleration. Now in the first step, because we're only using 1000 GS, we're going to separate things like the nuclei of the cells. So in the pellet we're going to have the nuclei and above in a supernatin, we're going to have everything else.