We see that Aspartate is here. We see that Glycine is here and here and so forth. Now, once these are actually down, right, what will happen next? Well, the binding process differs for each one of these amino acids. And that's because they have different charge values and they have different types of side chain groups. And what that means is when we actually wash our column down with some type of material, when we wash them down, they will basically detach depending on what the concentration, what the PH, and what the volume is of that particular buffer.