As the calcium ions build up, that will build up the positive charge and that will basically cause it will trigger these synaptic vesicles, synaptic vesicles, to fuse with the membrane of the cell. Now, each one of these synaptic vesicles is carrying hundreds and thousands of neurotransmitters. And in the case of the neuromuscular junction, the neurotransmitter is acetylcholine. So inside each one of these vesicles, we have acetylcholine. And as soon as we have a high concentration of calcium, that will cause these vesicles to undergo an exocytotic process and fuse with the membrane and release these neurotransmitters, ouraceetylcholine. Now, as soon as our acetylcholine are released into the space between these two cells, known as our synaptic cleft, they will begin to travel to the membrane of our postsynaptic cell, our muscle cell.