So, once again, from the graph, we see that 23 BPG helps unload more oxygen into the metabolically active tissue. At a partial pressure of about 40 mercury, the red curve shows a smaller percent saturation, about 60%, compared to the blue curve, which shows about 70%. And this implies that when 23 bifoxylglycerate is present, it is bound to deoxy hemoglobin, and it makes it much less likely to attach to oxygen. And so more oxygen actually unloads into those exercising tissues that have a high metabolic rate. So we conclude that just like increasing the temperature and increasing the concentration of carbon dioxide inside our blood plasma shifts the entire curve to the right, so does increasing the concentration of two three BPG. Now, if we decrease the concentration of two three BPG, the red curve will essentially shift to the left side.