So let's suppose we break it down into five different fragments and these fragments differ in their size. So we have the largest fragment of fragment A, we have the smallest fragment of fragment E and the fragment in between these fragments in terms of their size is fragment C. And that's the fragment, let's say, that contains the gene that we want to actually study. So fragment C is that DNA fragment, that restriction fragment that we actually want to identify and then separate. So let's move on to step two. So we take these fragments, we essentially place them into a solution that denatures the double helix structure. And so now in our solution we have these single stranded individual DNA molecules and now we take them and place them into a gel electrophoresis seven.