So we essentially pour our solution into our column. And so initially, at the initial moment we pour our solution, all of these proteins essentially congregate at the top of our column. Now, over time, what happens is because these two proteins have no affinity for the glucose on the beach, they will continue moving down our column as a result of the gravitational pull. But that protein, enzyme number one, that has the high affinity for glucose, will essentially bind onto that glucose molecule that is bound onto the beads. And this will happen ultimately at the beginning of our column. Now, some of these enzymes will make it farther down, but most of these enzymes bind in the beginning, towards the beginning of that column.