And once all the active sides are completely filled. This is when the enzyme mixture is operating at a maximum velocity, at a maximum rate. And in this moment in time, the concentration of the enzyme substrate complex, which is this here, is equal to the initial total concentration of that enzyme. So three is equal to three. So we can basically transform this equation to give us the maximum velocity, the maximum rate of that enzyme mixture simply by replacing the enzyme substrate concentration with the e total concentration. And this is given by this equation here.