And so once we form this intermediate, this is a trans delta two enol coenzyme A, as we saw in this particular case. Now this can basically complete that beta oxidation pathway to basically form a CIS delta four enol coenzyme A molecule that now contains only one double bond, as can be seen between the carbon four and carbon five. Now, this molecule is a substrate to two acyl coenzyme a dehydrogenase. And once the ACL coenzyme a dehydrogenase acts acts on this molecule, it forms a double bond between carbon two and carbon three. And so he formed this molecule, which we call the 24 dienol coenzyme a intermediate. Now, the problem with this molecule is this molecule is not a substrate molecule to enol coenzyme a hydrate.