So to see what we mean, let's take a look at the following diagram. So, we have the donor cell or the positive cell, the f positive, that contains this small circular f plasma shown in green. So when our two cells come in close proximity, this f plasmid is used to build the protein that is involved in the cytoplasmic bridge in the sex Pillus. And so that connects our two cells at the same time. The f plasmid is also replicated. And once it's replicated, we transfer it onto this cell.