Now, what we know about these three proteins is one of those proteins binds to a specific molecule, let's say a glucose molecule, and the other two enzymes, proteins, do not bind to enzyme molecules. So we have a green molecule, which is enzyme number one, that has a high affinity for glucose. So it basically has an active side that can accommodate a glucose molecule. We have enzyme number two in red that does not bind to glucose, and also enzyme number three in orange that also does not bind to glucose. So we take the beaker of these mixture proteins, we essentially dump them into our column, and they begin to move along our column. Now, only those enzymes that have the active side that can accommodate and bind onto the glucose will actually bind onto the glucose.