Now, once this reaction takes place, this oxygen of the Serene and the active side is oscillated. So this entire group is attached onto the oxygen as shown and this is the Covalent bond that is temporarily formed. Remember, Chimetrypsin uses Covalent catalysis and that means we form a temporary Covalent bond. So this intermediate is known as the acid enzyme intermediate. And in the next step we have the water molecule that joins in the active side and it basically hydrolyzes the cleavage of the peptide bond. So it ultimately breaks this bond here and informs the bond between the oxygen and this carbon.