And by going into that pocket, the negative charges of this two three PPG can interact with the positive charges of the residues found on the beta one and the beta two subunits. And if we zoom in on this interaction so this black molecule is the two, three BPG. If we zoom in on this molecule, this is what we get. So this is histidine 141 Lysine 82 and histidine two. These are the three residues that contain the positive charges found on the beta one subunits located at the center of the hemoglobin. And these are the three residues, HistoGene two Lysine 82 and HistoGene 143 that are found on the beta two subunits.