And we do this for a specific region because this is an important part of the process of diagonal electrophoresis as we'll see in just a moment. So these are basically our steps in diagonal electrophoresis. Now once we have these four fragments in our mixture in our solution we now place them onto a sheet and we run electrophoresis on that sheet along the horizontal direction. So we now place the fragments onto the corner of a sheet and we run electrophoresis along the horizontal direction. So essentially we place them onto the corner and they begin to migrate. And once again the ones that are heaviest, the ones that are largest will essentially migrate the least.