And that's essentially the point where exactly half of those DNA molecules or exactly half of a DNA molecule, has associated its double helix structure. Now, as I mentioned earlier, in the double helix, the bases are interacting via non Covalent bonds. And this interferes with the ability of those bases to actually absorb the light. And as we increase the temperature, we essentially cause the bases to break those bonds. And so once they're free, they can essentially absorb more light than before. And that's exactly what we see in the following diagram.