So, to see exactly how we can basically carry out this experiment with these three proteins. Let's take a look at the following diagram. So we have diagram A through E, and let's begin with diagram A. So in diagram A, we take our solution of three proteins and we pour it into our funnel in diagram B. That protein mixture, at the initial moment that we pour it in, basically collects at the top of the column and it has not separated yet. So this is our protein mixture that consists of three proteins.