So what happens is, when we move on to step two, in step two, at the top of our column, we have this collection, the mixture of three different types of proteins. So initially, they have not yet separated because they have not yet traveled through the section of our column through the beads. Now, as we begin to wait, what happens is these proteins begin to travel. They are pulled by the force of gravity. So as they travel, those tiny proteins, the green proteins, will travel slowest because they have to travel through the tiny pores of those beads, while the red proteins will travel slightly quicker, because sometimes they get stuck inside the beads. And have to travel through the beads.