So in this case, we're going to use negatively charged beads. So this is our ion exchange chromatography technique. And we take the beaker that consists of these amino acids in their individual form and we essentially pour it into our funnel so that eventually they end up in this column. And because these have charges, they will interact with the charges found inside our column. And so what will happen is they're going to bind the different sections of our column. So for example, we see that Alamine is somewhere here.