And then in the next step, we want to use that estimated PH value. In the second step, we want to find the average of the two PKA values right above and right below that estimated PH that we obtained in part one. Now, to actually see these two rules, these two steps in action, let's take a look at these two examples, beginning with example one. So find the pi value, the isoelectric point of the tripepptide, aspartate glycine and glutamate. So let's begin by drawing our structure for this tripeptide. So on the left side, we have this amino acid.