So we take a bacterial plasma and we place it, we mix it with these genes and we make sure that we also mix the plasmids with the same exact restriction enzyme. And the reason we want to mix those plasmids with the same restriction enzyme is to make sure that the sticky ends that are produced are complementary to the sticky ends that are produced within this particular case. So that once we mix the bacterial plasmids with these genes, those genes can basically use their complementary sticky end and attach onto that bacterial plasma. And at the end we also add DNA ligase to basically make sure that our bonds are fully formed between these genes and our plasmids. So at the end of step two, we now have these four different types of plasmids where each one of these plasmids carries its own gene. So this carries the green gene, this carries the purple gene, this carries the red gene and this carries our orange gene.