So let's begin by discussing PBR three, two, two. Now, this was one of the first plasmids that was used to basically clone make many copies of the recombinant DNA molecules. Now, this is what the plasma actually looks like. So we have this circular, double stranded DNA molecule. The orange section basically describes non coding genes. But these colored regions, the blue section and the green section, describe a special gene that codes for special protein.