So once we have that beaker, and inside that beaker, we have the solution that consists of only this type of protein. What do we do first? Well, ultimately, what we want to do is we want to separate the amino acids, the different amino acids, into different compartments. And then we want to count up how many amino acids are found within each compartment. But to actually separate our amino acids, we first have to break the bonds holding these amino acids together in that polypeptide chain. So in the first step, we basically want to break the bonds.