So let's take this PBR three, two, two plasmid. And as shown on the board, so we have this green gene that codes for that protein that gives the cell resistance to ampicillin. And we have this blue gene that gives the cell resistance to tetracycline. Now, we can use a variety of different types of restriction enzymes and three restriction enzymes that we can use are shown on the board. So if we use the restriction enzyme ECoR one, then it will cleave at this non coding section. If we use the enzyme cell one, it will cleave right in this blue region.