So in this reaction, we essentially have a certain rate law. And the rate law, the expression that describes the rate at which this reaction takes place is given by this equation here. So the V knot, the rate at which the enzyme catalyzes this reaction, is equal to the product of the rate constant, k two, and the concentration of the enzyme substrate complex Es. So this is the equation that describes the rate at some concentration of Es. Now, what exactly is the equation that gives us the maximum velocity, the maximum rate v max of that particular enzyme? Well, to find what the maximum rate is V max, we have to basically assume that all the enzymes, all the initial enzymes that we begin with, contain all the active sites that are completely filled with the substrate.