Now, let's suppose instead of removing a large segment of that gene, a large segment of this plasma, we want to remove a much smaller segment, as shown in the following diagram. So instead of removing this segment, we only want to remove this small segment. Well, the way that we carry out this process is almost like this process, but it has a slightly different case. So what we have to use is something called an axonuclease. So we take our restriction enzyme and in step one we basically add the restriction enzyme that also cleaves on this side and this side. And so at the end, we basically produce the following linear DNA molecule.