So previously we discussed protein sequencing. So we said that if we want to sequence the amino acids in our protein, the first step is to break down that protein into smaller fragments. Once we break it down to smaller fragments by using proteolytic enzymes, we can then isolate the fragments and take the individual small fragment and use admin degradation on that fragment fragment to basically determine what that sequence in that smaller fragment is. Now, to actually order the different fragments together to find the correct order of the fragments, we have to use two or more different proteolytic enzymes to produce different sets of fragments. And then we can use the overlapping regions of those fragments in the two different sets to basically determine what that sequence is. Now, to see another example of what we mean, let's take a look at the following example.