So as we go this way, the acidity of our buffer basically decreases. It becomes more basic. At the same time, the volume increases going in that direction. So we see that the second amino acid basically is our g, and that's glycine. Then we see that the next one in line is Alanine, because this much volume is basically used at a specific type of PH. And we continue our process until we see that the next one is phenylalalamine.