Now, where do we get our supply of acetylco enzyme A molecules? Well, we obtain it in the matrix of the mitochondria via processes such as the beta oxygen oxidation of fatty acids. We also generate acetylco enzyme A molecules via the decarboxylation of pyruvate, the catabolism of certain amino acids, as well as the breakdown of ketone bodies. But ultimately, this acetylco enzyme A molecule is found in the matrix of the mitochondria. And that poses a problem, because fatty acid synthesis takes place in the cytoplasm of the cell. But these acetylco enzyme A molecules are located in the matrix of the mitochondria.