And we form three different fragments, as shown, that have these different sizes. Now, in case B, we take that same initial gene, but now we expose it to a different restriction enzyme which cuts at different locations along that gene. So now, instead of producing these three fragments, we only produce two fragments because this gene only contains one side, one location where this restriction enzyme number two can actually act on. So now we produce fragment four and fragment five. And once again, we expose these two fragments. We place these two fragments into our gel and now we have the separation based on size.