Now, do we go up or do we go down? So basically, because we have a charge of negative two, that means we want to decrease the amount of negative charge we have on our protein. Now, if we go higher, if we go above eight, we will remove this positive charge. And make our peptide more negative. So that means instead of going above seven, we have to go somewhere below seven to basically remove some of that negative charge. And so to remove the negative charge, right, we have to remove two negative charges because our charge at seven is negative two, we have to go below a PH of 4.1, because below a PH of 4.1, these two groups will gain an H atom and so will neutralize themselves.