And so we see that inside our red blood cells, we have this allosteric effector, the two three BPG molecule that binds onto a location other than the heme group. So it binds towards the center. And the entire purpose of it is to basically stabilize the t state, make the t state more stable and lower in energy, and that drives the curve towards the right side, decreasing the affinity of hemoglobin for oxygen. And what that means mathematically is much more of that hemoglobin will actually be able to unload and release the oxygen towards to the cells of our exercising tissue, as seen in the following calculation. So 66% compared to only 8% in the case of pure hemoglobin when we don't have the two three BPG. So, in the presence of two, three BPG, the two three BPG binds to the pocket, and it stabilizes the t state.