So basically, our tRNA molecule that is bound to our amino acid is known as the amino acid tRNA or the charged tRNA. And when somebody says it's an uncharged tRNA, that means the tRNA is no longer bound to our amino acid. So once our tRNA finds the methionine amino acid, it then brings that methionine amino acid to our mRNA molecule, and the tRNA binds to our mRNA molecule. And once that takes place, the large subunit of the ribosome binds the small subunit they associate, and they form a ribosome complex that is attached to that mRNA molecule. So basically, the initiation process involves these steps, and ultimately, we form the following ribosome complex. So we have the mRNA molecule that begins at the five end and ends on the three end.