And by changing our PH we essentially ionize the bases. And by ionizing the basis we disrupt we break the hydrogen bonds holding our nucleic acids in that double helix structure. Now, we can also heat our solution. So by heating the solution we're essentially bringing energy into the solution. So we're increasing the energy and we're increasing the energy of the bases and that will essentially disrupt the hydrogen bonds breaking and dissociating that double helix structure. So in a lab, we can either heat our solution by increasing in temperature or we can change the PH.