And at the end we also add DNA ligase to basically make sure that our bonds are fully formed between these genes and our plasmids. So at the end of step two, we now have these four different types of plasmids where each one of these plasmids carries its own gene. So this carries the green gene, this carries the purple gene, this carries the red gene and this carries our orange gene. Where these sections here are our genes and these brown sections are the rest of the DNA sequence of that particular plasmid. Now also notice that the plasmid comes with a special blue section. And what the blue section is, it's a special gene that codes for a special protein that gives the bacterial cell resistance to drugs, resistance to antibiotics.