So by heating the solution we're essentially bringing energy into the solution. So we're increasing the energy and we're increasing the energy of the bases and that will essentially disrupt the hydrogen bonds breaking and dissociating that double helix structure. So in a lab, we can either heat our solution by increasing in temperature or we can change the PH. And either one of these will basically break all these hydrogen bonds holding our two strands of DNA together. Eventually we dissociate our DNA helix and we form these two individual strands of DNA. So this is basically the backbone that consists of the phosphate and sugar groups and these color structures are our bases.