And the way that this is usually done is we choose some specific type of procedure. We carry that procedure out and then we extract that sample and we place that sample into an SDS page setup. So we expose our sample to SDS polyacrylamide gel electrophoresis to basically separate our proteins based on size. And as we go from one step to another step, if the procedure is working we have to get less bands and less bands and less bands until we get a single band as we'll see in just the moment. And that basically means we're purifying our sample because we're removing some of those proteins with specific mass values as we'll see in just a moment. And every time we conduct a procedure we also calculate these five quantities.