So in this diagram we have the DNA gyrase. So the DNA gyrase binds to our double strand DNA molecule and it creates the negative super coils which decrease the number of positive super coils in our double stranded DNA molecule. Now, the problem with separating our two single strand of DNA is the following once we separate our two single strands of DNA, they want to naturally and spontaneously reform those hydrogen bonds because by reforming the hydrogen bonds that stabilizes the double stranded DNA molecules. So we see that to keep the two single strands apart during DNA replication, another type of enzyme known as the single stranded binding protein has to bind to these individual exposed regions of the single strands of DNA. So these single stranded binding proteins, also known as SSB proteins, are shown in purple on this molecule. And when they bind, they basically keep these two single strands of DNA from reassociating with one another, from reforming the hydrogen bonds.