So the largest fragments, fragment A, will be all the way at the top because it will experience the greatest resistance, while the smallest fragment, fragment e, will be found all the way at the bottom because it does not experience a large resistive force. And once we separate the five fragments based on size, we can then basically transfer that result onto a special polymer sheet that we can use more effectively. And usually we use the nitrocellulose polymer sheet. Now, once again, it's important to know that within these regions, these are no longer in their double stranded form, they exist as single stranded DNA molecules. And that leads us directly into step three. So in step two, we essentially separated these fragments based on size.