OligA simply means we have about ten to 20 to 30 of these nucleotides found inside that DNA molecule. So we synthesize an artificial oligonucleotide in the lab, and then we change the sequence ever so slightly to basically accommodate a codon that will create the aspartate instead of glutamate. Now, let's actually take a look at the following three steps that we have to follow to basically form that protein that will contain the aspartate instead of the glutamate. So, in step one, we basically take that plasma double stranded DNA molecule that contains this DNA template that we studied in this diagram. And so this is our plasmid. Notice the plasma contains the same DNA sequence that we discussed in this diagram.