So this is our plasmid of some particular DNA molecule. So this is one DNA molecule. And suppose that this is our target DNA, the recombinant DNA that we form, that we basically want to amplify. So what we do now is we take these two different sources of DNA, we add a restriction enzyme, so we cut them at specific locations and then we add DNA ligase to basically produce this recombinant plasmid that contains this blue section that we basically want to amplify. So this is the same exact process that we followed right over here. And now we take this recombinant plasmid and we insert it into bacterial cell.