Let's move on to the next one. So we're at Glutamate. For Glutamate, the PK is 4.1, and since five is above 4.1, that means this oxygen will have lost that H atom and so it will have a negative charge. So finally we have at least one negative charge on our peptide. Let's move on to the next 116. So for 16, the PK is 8.3, which is above the PH of five.