Now, as we begin to increase our temperature or as we begin to increase the amount of chemical agent, what begins to happen? Well, initially nothing really happens. A small amount of the protein begins to unfold. But as we approach this section, we see a sharp increase in the percentage of the protein that have denatured. Eventually we basically reach this point where all the protein exists in their fully unfolded denatured state. So the curve that we get is a sigmoidal curve.