And so for this not to actually take place, something else must be done after glycolysis takes place. So something must be done to regenerate that coenzyme the NAD plus that is necessary for the activity of gap dehydrogenase that basically catalyzes step six of glycolysis. So once again, in step six of glycolysis, NAD plus nicotine amide adenine dinucleotide in the oxidized form, this is the molecule that comes from niacin, vitamin B three. Basically, this molecule acts as a coenzyme and helps gap dehydrogenates to transform the gap molecule glyceroaldehyde three phosphate into one three bisphospho bisphosphoglycerate. And in this process, we see that NAD plus was used up and it was never actually regenerated in the remaining steps of glycolysis. And so this means that glycolysis basically depends on NAD plus.