The fourth band consists of at least three proteins, and the fifth band consists of at least five different proteins. Now, it's not to say that we don't have more proteins here. For example, the reason to say we have at least five proteins in this section is because two proteins that have the same exact isoelectric point can also have the same exact mass. And so each one of these sections, each one of these individual bands can consist of two or more proteins, but it also can consist of only a single protein. To basically determine if we have more proteins in each one of these sections, we have to carry out some other type of purification process that separates the proteins based on some other type of property. So in the second method, the horizontal gel lane that contains the protein, so this entire gel lane is placed onto the SDS Page apparatus.