So this one here, Lysine, PKA of 10.8, which is above seven, that means it will have a charge of positive one on that nitrogen. We move on to the next amino acid in line, which is glutamate has a PK of 4.1, which is below our neutral PH of seven. So this will bear a negative charge on the oxygens here. So we have a negative charge here. On the next one, we have cysteine. And so for cysteine, we have a PKA of 8.3 above seven.