And since two is below 8.0, that means this will have a positive charge. So once again, remember that what the PKA value tells us is so if the PH is equal to the PKA, that is when our H atom will be lost or gained. And in this particular case, at a PH of eight or higher, this is when the H atom on this nitrogen will be lost. So our first charge is a positive one charge. Now let's move on to the second group. This group here, this is HistoGene.