And that means if we insert that fragment into this section, it will inactivate that blue gene. And likewise, we can also use a third restriction enzyme known as PST one. And if we use that, it will make a cut right here and insert that fragment into this section, thereby inactivating this green gene that codes for the protein against ampicillin. So let's suppose this is the DNA fragment that recombinant DNA, that novel DNA that we create in a lab that we want to replicate. So let's suppose we want to insert it into this plasmid. And let's say we use the PSTI, the PST one restriction enzyme.