Now, to begin the process of replication, the double stranded DNA molecule must actually separate. So we have to break the hydrogen bonds. Now what's one way to break the hydrogen bonds between the bases? Well, one way to break the bonds is to heat our solution that contains that double stranded DNA. So if we heat our solution with that double stranded DNA molecule of interest to a temperature of 95 degrees Celsius for about 15 seconds, then that's just enough time to basically break all those bonds between our two strands and separate our two strands. Now the next process is we want to actually anneal those DNA primers that we form.