And this usually creates a very high specific activity value because as we know, enzymes only bind to specific substrate to specific molecules. So let's see if that's true by taking the extracted mixture and placing it into our last well. So what we produce is a band distribution that only contains a single band. And what that usually means is we have isolated that protein of interest because this band consists of a protein that has a specific type of mass, a specific type of size. Now let's owe and by the way, let's calculate what the yield in this case was. To calculate the yield, we follow this equation.