We have enzyme number two in red that does not bind to glucose, and also enzyme number three in orange that also does not bind to glucose. So we take the beaker of these mixture proteins, we essentially dump them into our column, and they begin to move along our column. Now, only those enzymes that have the active side that can accommodate and bind onto the glucose will actually bind onto the glucose. And so only these green molecules will become trapped because they're bound onto the glucose of the bees. And the other two enzymes, enzyme two and three, will not interact with the glucose in any way. So they will simply continue traveling along and down our column.