The second step is a hydration step. The third step is another oxidation step, but now it is oxidized by NAD Plus. And the final step is a thiolytic cleavage bico enzyme, a molecule. So let's begin by focusing on the first step, the oxidation of flavin oxidation by flavin adenine dinucleotide. So we begin with our fatty acid in the acyl coenzyme A form. So this is the activated form of the fatty acid and an enzyme known as acyl coenzyme adhdrogenase basically catalyzes the formation of a double bond between carbon two, the alpha carbon, and carbon three, the beta carbon.