At the same time, E Two and enzyme E Three basically depart and we form this final product. Now, this is not actually the final step of this reaction. In order to actually target that particular protein for degradation, we have to actually add many more Ubiquitant molecules. In fact, we have to add at least four ubiquitous molecules to actually target that protein for degradation. And the way the Ubiquitin is added is in a process of manner. And what that means is once we attach that Ubiquitin onto this protein, we actually attach more ubiquitous molecules onto this ubiquitin.