In fact, ATP will act as an allosteric inhibitor of isocytrade dehydrogenase, and that will decrease the affinity for the substrate. In addition, the NADH will kick off the NAD plus that is needed for step three A to take place, and that will inhibit the activity of ISOC citrate dehydrogenase, decrease the rate at which this step actually takes place, and that will decrease the rate of the citric acid cycle. So let's take a look at the following diagram to see exactly what we mean. So the Pyruvate is transformed into acetyl coenzyme A via pyruvate carboxylation. The citrate then is produced. And once the citrate is produced, it is isomerized into isocitrate.