So we see that if we continue going higher, at a PH of around four, five, six and seven, our amino acid exists in its Zitter ion form. That means we have a positive charge here. We have a negative charge here. Now, as we continue driving the PH up, as we continue decreasing the amount of H plus ions in our solution, eventually we get to the point where we have so little H plus ions in solution that this H found on a nitrogen is going to deprotonate. It will dissociate as to increase the amount of H plus ions in solution. And at around a PH of nine, that is exactly where that begins to happen.