And so notice as we go from the native state to our denatured state, these interactions take place cooperatively. And not only that, but we also go through these intermediate states. And so what that means is during the folding or unfolding process, going this way is unfolding, going this way in reverse, is folding. During either process, proteins follow a partly defined pathway that consists of these intermediate states. And so what we see in this particular case, we have two intermediate states, but we could also have five intermediate states, ten intermediate states and so forth. And what these intermediate states basically describe is that is they describe energy states.