So proteolytic cleavage simply means we have some other type of protein that cleaves bonds within that zymogen. So this process involves the hydrolysis of one or several peptide bonds within that Zymogen enzyme. Now Proteolytic cleavage doesn't necessarily require ATP, a source of energy, and that means that proteolytic cleavage can take place outside of the cell. Now proteolytic cleavage is also a permanent and irreversible process and that basically means that it only occurs once in the life cycle of that enzyme. Now, what are some examples of our body using Proteolytic Activation? Well, the process of blood clotting is one example of a biological process that involves a series of proteolytic cleavages.