What the graph basically describes is the percentage of the proteins that are denatured versus the conditions that the protein is actually under. So as we go from left to right along the x axis, we either increase our temperature of the solution or we increase the amount of chemical denaturing agent that is found within that mixture. So when no chemical agents are found in the solution and the temperature is normal, 0% of the proteins are denatured, and all the proteins essentially exist in their fully functional threedimensional folded native states. Now, as we begin to increase our temperature or as we begin to increase the amount of chemical agent, what begins to happen? Well, initially nothing really happens. A small amount of the protein begins to unfold.