So finally we have at least one negative charge on our peptide. Let's move on to the next 116. So for 16, the PK is 8.3, which is above the PH of five. And so that means this will have a net charge of zero. And for this particular case, because we're at a PH of five, which is above this, this will also have lost that H atom and so it will have a negative charge. So we have two negative charges and we have three positive charges.