We divide that by two, we get 5.55. So the pi value for glycine is 5.55, assuming these are our PKA values. Now these PKA values might change if we change the conditions under which this amino acid is in. So in your textbook, or maybe your teacher might give you different PKA values. And that's because the temperature conditions or other conditions under which that amino acid exists in are different. So let's move on to the second case.