And this continues until the polypeptide chain is actually complete. Now, the next question is, how do we know that our polypeptide chain is actually complete? Well, the last stage of translation is known as termination. And just like there is a start codon that basically signals the process of initiation, there is also a codon known as the stop codon that signals the process of termination, where we stop the addition of our amino acid to the growing polypeptide chain. So if the ribosome reads either the UAA, the UGA, or the UAG sequence a protein called the release factor protein will bind to the aside instead of a tRNA molecule. And this will ultimately cause the growing polypeptide chain to actually break off from our tRNA molecule and eventually the ribosome complex will dissociate and will create that polypeptide chain.