How exactly does the protein unfold from the native state into that denatured state? Or how does a protein fold from a denatured state and to that fully functional threedimensional state? So, let's begin by taking a look at the following graph. What the graph basically describes is the percentage of the proteins that are denatured versus the conditions that the protein is actually under. So as we go from left to right along the x axis, we either increase our temperature of the solution or we increase the amount of chemical denaturing agent that is found within that mixture. So when no chemical agents are found in the solution and the temperature is normal, 0% of the proteins are denatured, and all the proteins essentially exist in their fully functional threedimensional folded native states.