And what that means mathematically is much more of that hemoglobin will actually be able to unload and release the oxygen towards to the cells of our exercising tissue, as seen in the following calculation. So 66% compared to only 8% in the case of pure hemoglobin when we don't have the two three BPG. So, in the presence of two, three BPG, the two three BPG binds to the pocket, and it stabilizes the t state. Now, when we increase the concentration of oxygen, for example, when the deoxy hemoglobin in the t state with a two, three BPG returns from those tissues back to the lungs, the concentration of oxygen increases. And as the concentration of oxygen increases, the oxygen begins to slowly bind onto the heme groups of these different polypeptides in the hemoglobin molecule. And as oxygen begins to bind to the heme groups, that basically creates a conformational change.