So when all the active sites are occupied, that means this quantity, the concentration of this will equal to the total concentration of the enzyme. And that can be seen from this particular equation seven. So in this equation we see that when this value is equal to zero, that means all the enzymes active sites are filled with a substrate, because when this is equal to zero, this will equal to this. And so what that means is the V max is equal to K two multiplied by the maximum value of the enzyme substrate complex concentration. And this maximum value can be a maximum of E total. So we replace this with this quantity here.