And so four will be here and one will be here. So following electrophoresis in the horizontal direction this is our distribution of proteins, of bands that we actually get. And notice that protein three consists of a blue section, this section and the green section, this section here. So let's call this blue section, let's call it three B. And let's call this green section, three A because that will become important in just a moment. So this is step A of diagonal electrophoresis.