So, in step one, we basically take that plasma double stranded DNA molecule that contains this DNA template that we studied in this diagram. And so this is our plasmid. Notice the plasma contains the same DNA sequence that we discussed in this diagram. And so before we can replicate, we actually have to separate these two strands of DNA and we have to isolate this DNA template of interest. So we basically heat our solution that contains this plasma, and then we separate one of the strands of DNA. So this strand right over here, the inner one, which has the sequence tat, GCC, CTT, TGC, CCT, the same sequence that we discussed here.