On top of that, we can also say that the nitrogen has a partial positive charge, a partial negative charge, because nitrogen is more electronegative than the nearby carbons. And so we can say there is this partial negative charge that exists on this nitrogen. And so it will interact with the H atom because this H atom of this alcohol of the Serine contains a partial positive charge because this oxygen contains a partial negative charge because of its high electronegativity value. And so Aspartate 102 basically interacts with Histazine 57 to move it and position it into the correct orientation so that the lone pair of electrons on the nitrogen can now interact and pull away this H atom. Now, once the H atom is pulled away, that transforms that alcohol group into an alcoxide group. And because the alkoxide is a much stronger nuclear file, this will now interact with the carbon of the carbonyl and essentially break that peptide bond as we'll see in just a moment.