But notice what happened to three, because three, after we exposed the two performic acid has been separated because these two bonds broke. Three now consists of three A and three B that are not connected. And so when we run gelature freezes along the vertical direction, what happens is they will actually separate because three A is much smaller than three B. And so three A will essentially travel higher up than three B and three B because it is the largest out of all. These fragments will be found lowest along the vertical direction. And so now we know that there exists disulfide bonds between these two fragments, three A and three B.