They bind to a special side between the two beta subunes of hemoglobin, and they create a conformational change. And by binding to hemoglobin and creating that conformational change, they decreased a lower vicinity of hemoglobin for oxygen. And once again, this allows for more oxygen to actually be unloaded, and more oxygen ends up in the cells of our tissue. And once again, increasing the concentration of two, three BPG will cause a rise work shift on our oxygen hemoglobin dissociation curve. So we see that increasing four of these different things will shift the curve to the right, and that will make it more likely for a hemoglobin to unload that oxygen and deliver more oxygen to the cells of our tissues. So we have increase in H plus ion concentration, increase in temperature, increase in the concentration of CO2, and increase in two, three BPG.