So now we produce fragment four and fragment five. And once again, we expose these two fragments. We place these two fragments into our gel and now we have the separation based on size. And we can compare this diagram to this diagram and we can use the information obtained to basically create a restriction map. So if we examine the following diagram right over here, we see that for this particular case, this fragment is this fragment here. And notice it is closest to the anode because it is the smallest.