Now, in the next two steps, what we ultimately want to achieve is we want to build that six membered primidine ring, as shown here, that we're ultimately going to use to attach onto a sugar molecule to form that perimedine nucleotide. So in this step, we basically have a dehydration step. We essentially release a water molecule. So we have one H plus ion coming in. We take off one H atom from this nitrogen and we use this oxygen to basically form this water molecule. In the process, we form a sigma bond between this nitrogen and this carbon.