That's why we have the origin of replication. So the PBR three, two, two plasmid contains genes that give it resistance to antibiotics. Ampicillin, the green one and tetracycline, that blue one. So how exactly can we take that novel gene, the recombinant gene that we create in a lab, and insert it inside that plasma? Well, we have to use restriction enzymes. Restriction and the nucleases, these are the enzymes that are able to actually cleave our DNA molecule at specific locations.