And so that makes this last protein unnegatively charged protein, contains a net negative charge of negative one. So the question is, when we place the mixture of proteins into the column that contains these negatively charged cellulose beets, what exactly will happen? What will be the rate of movement of each of one of these proteins? Well, as you might know, the positively charged proteins will be attracted to the negatively charged beats beads. And the more positive charge we have, we know by Coulomb's law, the greater that attraction will be. And so what that means is these very positive, these proteins that have a very large net positive charge will be attracted to those beads.