And since where at a PH of seven, what that means is this will have lost the H atom, it will have a neutral charge. And so now we have a charge of zero for the second case, for that first side chain group. Now the second side chain group and the second amino acid is tyrosine. And so we have a PKA of 10.9, which is above seven. And so what that means this will have a neutral charge, just like in the other two cases. Let's move on to the third amino acid.