So we can use a buffer, for example, sodium side trait of increasing PH to basically wash down our column. And as we wash down the column, these amino acids will begin to elude at different rates. So the identity of the amino acid can be determined based on the volume of the buffer and the PH of the buffer that is needed to actually elude that amino acid. So we essentially have these values that we can find in a textbook and then we can actually determine what the values that we use are, and then we can compare them to those textbook values and see exactly what amino acid we are dealing with. So, to see what we mean, let's suppose we use a buffer at a relatively low PH, and we use a very small amount of that volume before we see that one of those amino acids actually eludes. And so if we know if we calculate how much volume we used and what the PH of that is, and we compare to our textbook value, we'll see that the first amino acid was this green amino acid, which is aspartate.