It represents the highest, the maximum energy value in that particular chemical reaction. And this Daggert symbol describes the energy state. So to calculate the free energy value of the transition stage, of that molecule, of that chemical reaction, we simply take this Y coordinate value and we subtract the energy of the reactants. So the energy of the transition state minus the energy of the reactants gives us this quantity known as the free energy of activation or simply the activation energy, the activation barrier of this particular chemical reaction. Now, what happens is when the enzyme actually takes in these molecules. So we have the enzyme in the enzyme.