So let's suppose this is the DNA fragment that recombinant DNA, that novel DNA that we create in a lab that we want to replicate. So let's suppose we want to insert it into this plasmid. And let's say we use the PSTI, the PST one restriction enzyme. So that means this is where we cleave it. And if we use this same restriction enzyme and this same DNA molecule, we can insert it into this region. And so we produce the following plasmid.