The second method does not always work. Why is that? Well, that's because there is another difference that exists between prokaryotic and eukaryotic cells. Unlike in eukaryotic cells, in prokaryotic cells, once the protein is synthesized following translation, that protein is not modified in any way. But in many eukaryotic cells, many eukaryotic proteins are actually modified in different ways in the Golgi apparatus following translation. And so what that means is we can add different types of components onto our protein, for example, sugar molecules and lipid molecules.