Now, in step two, we now create that DNA primer that contains that point mutation and we add it into our solution at the right temperature so that the kneeling process will take place. And so now the DNA primer will essentially hybridize with this section of that DNA template that we're going to use to basically synthesize our polynucleotide chain. And now we add our DNA polymerase along with the four types of building blocks, the four types of deoxy nucleus cytrophosphate molecules. And now the DNA polymerase will bind onto the primer and begin synthesizing in the five to three direction from this end to this end. So it will move all the way along this DNA template. And we'll use that DNA template to synthesize that new strand of DNA that now contains this codon sequence GAC that codes for glutamate.