Now, for one NADH that is formed in glycolysis which takes place in the cytoplasm of the cell a net of two ATP molecules are produced. And that's because a single ATP must be used to actually transport that NADH from the location weglisis takes place the cytoplasm into the mitochondrial matrix where our electron transport chain is found. Now, what about our Fadh two? Well, Fadh two is not formed in glycolysis and it's not formed in pyruvate decarboxylation. It's only formed in the citric acid cycle. In fact, our Fadh two is formed directly in protein complex number two known as succinate oxidoreductase that is found on the electron transport chain.