So, we see not only does two three BPG shift the curve to the right side and lower the affinity of hemoglobin for oxygen, but so does the hydrogen ion concentration and the carbon dioxide concentration. And these three molecules, 23 BPG, H plus ions, and CO2 molecules together create a very effective hemoglobin molecule that is able to actually unload a lot of many of those oxygen molecules to the tissues of our body. So a higher concentration of CO2 and a lower PH, meaning a higher amount of H plus ions. Because remember, as we increase the H plus ions, we decrease our PH. So these two things shift the curve to the right, thereby decreasing the affinity of hemoglobin for oxygen and allowing the hemoglobin to unload or release more of those oxygen molecules into the exercising tissues, the cells of our body. Now, how does this actually take place?