So if there is a high concentration of calcium for a very long period of time in a cytoplasm, that can actually cause damage, that can actually lead to the precipitation of many different types of proteins within that side of plasma. So during steady state conditions inside the cell, there is a very low concentration of calcium, about 100, that's much, much smaller than the outside concentration. And this is because calcium ions can readily bind to the negatively charged regions of proteins forming insoluble complexes that can be harmful to the cell. Again, when the calcium binds to the protein, it usually exposes hydrophobic regions of the protein. And we'll see why that's important in just a moment. But if that continues to happen for a very long period of time inside the cytoplasm that can be harmful.