So when the protein reaches its specific isoelectric point, the pi value, it will be, it will stop moving within that gel. And so if we, for example, have a mixture of three proteins that each have their own unique ISO electric point value, and we place them into our mixture, they will separate until they will separate, and they will stop moving when they reach their pi value. So for protein one, the pi value is acidic, or relatively acidic. For protein three, the pi value is relatively basic. And for protein two, it's somewhere in the middle, so it's essentially neutral. So once again, in isoelectric focusing, a gel with a PH gradient is created.