Now, the negative simply means it's to the left. It's upstream with respect to that bacterial gene. And this sequence is known as the primal box. So the primal box contains a consensus sequence, a sequence that doesn't change when we go from one organism to another organism. And the sequence is Tat, where T is thymine and A is adenine. Now, we have another promoter that has found 35 nucleotides upstream to the left of that bacterial gene and this contains a consensus sequence of TTGACA.