What will happen is these two strands. So now we have two strands, we have two double helices of these DNA molecules. We increase the temperature so that they essentially separate. Then we drop the temperature to 54 degrees Celsius. We still have the primers in solution. Those primers will essentially hybridize with the proper ends, and then we increase the temperature back to 72 degrees Celsius so that that's the temperature where DNA polymerase can begin its replication process.