So let's suppose that this is our setup. So this is the funnel placed on top of our column. Inside the column, we have these specially formed gel beads that essentially contain a charge. So in this case, we're going to use negatively charged beads. So this is our ion exchange chromatography technique. And we take the beaker that consists of these amino acids in their individual form and we essentially pour it into our funnel so that eventually they end up in this column.