Now, we can use a variety of different types of restriction enzymes and three restriction enzymes that we can use are shown on the board. So if we use the restriction enzyme ECoR one, then it will cleave at this non coding section. If we use the enzyme cell one, it will cleave right in this blue region. And that means if we insert that fragment into this section, it will inactivate that blue gene. And likewise, we can also use a third restriction enzyme known as PST one. And if we use that, it will make a cut right here and insert that fragment into this section, thereby inactivating this green gene that codes for the protein against ampicillin.