So, once again, as a mixture of proteins travels through our column, the small proteins into the porous beads, but the larger proteins cannot fit into the internal volume of those beads. And as a result, those large proteins will end up at the bottom first, while the small proteins will emerge less. So let's see how we can actually carry out this experiment with these three different proteins by using gel filtration chromatography. So let's take a look at the following three diagrams. So let's begin with diagram one. In diagram one, we have the beaker.