Well, once again, let's suppose we have the following segment or the following plasma. So on the plasmid we have this orange section that we essentially want to keep and we want to remove this brown section. And then we want to insert a specific segment of DNA that we essentially synthesized in the laboratory. Now, the method that we're going to use is known as the Cassette Mutagenesis method. So a procedure called Cassette Mutagenesis can be used to insert specific segments of DNA into the plasmid known as cassettes. So we essentially take our plasma and as always, we mix it with our restriction enzymes.