And so in step four, we essentially turn the knob and we open up our hole. And so that allows the movement of these two proteins that we did not want to separate in the first place. And we basically place them in this beaker. And so now we can dump that out because we don't want to actually use this in our experiment, remember, our focus was basically to isolate protein number one and not protein number two or protein number three. Okay? And finally, in the last step, so the question is, how do we get that protein number one out of that column?