So we see that the second amino acid basically is our g, and that's glycine. Then we see that the next one in line is Alanine, because this much volume is basically used at a specific type of PH. And we continue our process until we see that the next one is phenylalalamine. And finally, all the way here, we have the r, which is arginine. And that makes sense because arginine is the most basic and aspartate is the most acidic. So step two basically allows us to actually separate our amino acids into different containers.