And this is the anameric carbon and it's the beta anomer because the hydroxyl group on carbon one points in the same direction as this group attached to carbon number five. So in the process of phosphorylation, when we transform glucose into glucose six phosphate, we essentially add the phosphoryl group onto carbon number six, as shown in this diagram. Now, what's the major difference between the glucose and the glucose six phosphate? Well, the major difference is the presence of this modified group. And this group contains a net negative charge, specifically a charge of negative two. So compared to this glucose molecule, which has a net charge of zero, this molecule has a net negative charge.