Now, before we actually examine what these experiments were, let's take a look at some important molecules and reagents that he used in his experiment. So he used two important denaturing agents. Denaturing agents are these molecules that basically break down the structure of our protein. So he used urea and betama capto ethanol. Now, urea is basically used to break down the non covalent bonds, such as hydrogen bonds and ionic bonds that hold together that secondary structure of the protein as well as parts of the tertiary structure. And he used bader mercaptoethanol to basically break down the covalent bonds, the disulfide bonds that exist and hold that tertiary structure of the protein together.