And let's use blue to basically designate that. So notice, even though the yield decreased, it didn't decrease by as much. And so that's a good thing because that means we're not losing the majority of the protein. Now let's move on to C. In C, we take that extracted mixture following salting out and we expose that to ion exchange chromatography. And an ion exchange chromatography. We basically separate our protein mixture based on the net charge.