And so at this particular PH value, this will have a net charge of zero. Now let's move on to case number three. Let's suppose we have an ionizable amino acid, but it is basic. So that means there are three different amino acids that fit this category. So we have Lysine, we have arginine and we have HistoGene. Now in this particular case, what we have to do is we basically take the sum of the PKA value of that side chain group and the PKA value of the alpha amino group.