And if we have a high amount of ATP, that usually means we have an unequal distribution of the perimeterine to purine nucleocide triphosphates. And to equalize this distribution of nucleotide triphosphates perimeterine to purine nucleotide triphosphates, we have to carry out this reaction to actually increase the amount of CTP, the perimedidine nucleocide triphosphate that is found inside our cell. So physiologically, a high ATP concentration means that there is an unequal distribution of the nucleocide triphosphates, the purine to perimeidine nucleocide triphosphates, and therefore, the cell tends to equalize this distribution by producing more perimedine nucleotide triphosphates, such as CTT. So we basically conclude that the reason this enzyme displays Cooperativity is because the individual subunits of this enzyme actually interact with one another. And by binding of the substrate molecule, we essentially shift the equilibrium from the T state to the R state. Now, in order to regulate the activity of this enzyme inside our cells, in order to actually turn on or turn off the activity of the cell of this enzyme, our cells use these two regulatory effectors.