So another more versatile plasma that we can use as a vector to basically amplify recombinant DNA molecules is the PUC one eight. And just like this plasma here, this plasma also contains an origin of replication. And that means we have a beginning. The DNA polymerase will know exactly where to begin replication. And just like this one, this one also contains the ampicillin resistance gene. So that means we can use this gene as a marker to basically differentiate between those sounds that did not take up the plasma and those cells that did.