So there should be a bond here, and tyrosine Lysine tells us that Tyrosine, Lysine, there should be a bond here. So we can piece all this together to basically write down what the final amino acid sequence is, where is in my pocket. Okay, so if we write down the final sequence, we get Glycine tryptophan, then we have a bond here because of these overlapping regions. So we have Arginine, then we have Tyrosine, and then based on these overlapping regions here, we have Tyrosine and Lysine are connected, and so then we have Aspartate and Serene. So we have 123-4567, which is our initial seven amino acid polypeptide chain. So this is basically how you sequence the proteins, how you use these different types of proteolytic agents to produce different sets of fragments.