This will separate the proteins that have identical pi values based on their different masses, different sizes. So we conclude that the two dimensional gel electrofreezes process is a highly effective method that separates the mixtures of the mixture proteins based on two different properties, so their isoelectric point and their size. So the horizontal direction is the isoelectric point. It separates the proteins based on the PH at which they have a net charge of zero, and the second vertical dimension basically separates them based on size. So this is the process of two dimensional gel electrophoresis. It combines isoelectric focusing and st polyacrylamide gel electrophree.