So let's begin with deletions. Now, deletions can be broken down into two categories. We can delete very large segments of the gene, or we can delete smaller segments of the gene. And these two categories basically require slightly different methods. So let's begin with method number one, by which we essentially modify our plasmid by removing a large segment of DNA from that plasmid. And the way that we carry out this process is so we take our plasmid.