But as we approach this section, we see a sharp increase in the percentage of the protein that have denatured. Eventually we basically reach this point where all the protein exists in their fully unfolded denatured state. So the curve that we get is a sigmoidal curve. So we have a sharp transition from the folded to the unfolded. And we can also look at the graph going the other way. So as we decrease the temperature, as we return it back to normal, or we remove those chemical agents, we see a sharp transition from the denatured to that fully folded active state of that protein.