So let's discuss what actually takes place during this process. So, as soon as the action potential comes to our synaptic terminal, it causes the calcium channels on the membrane to open up. As soon as our calcium channels open up, that will cause the influx of calcium ions into the cell. As the calcium ions build up, that will build up the positive charge and that will basically cause it will trigger these synaptic vesicles, synaptic vesicles, to fuse with the membrane of the cell. Now, each one of these synaptic vesicles is carrying hundreds and thousands of neurotransmitters. And in the case of the neuromuscular junction, the neurotransmitter is acetylcholine.