Then we have the A. Then we have the g then we have the E. So SD eight SDS page So isoelectric focusing can be combined with SDS polyacrylamide geelectrophrases, or SDS Page to create a more effective and more efficient way of purifying our crude mixture of proteins. And to see exactly what we mean, let's take a look at the following two steps. Now, we're not going to focus too much on each one of these steps because we spoke about these in detail in previous lectures. We're simply going to show you the fact that we can combine these two techniques to basically create a more effective method. So, in the first step, we take our crude mixture of proteins found in the following beaker, and we expose it to isoelectric focusing.