So that doesn't change. But now that we insert this three nucleotide sequence, this sequence CUG corresponds to the amino acid leucine. Now next we go on to GCU, which is the alanine MCGC, which is our arginine. Now notice what happened. There's a big difference between this result and this result. So because in this case, we had a frame shift mutation because we only inserted a single nucleotide that shifted the reading frame and that changed all the amino acids that were produced after our mutation.