We have the Co substrates, which are basically coenzymes that bind to our enzyme via loosely held bonds while our prosthetic group is basically the coenzyme that binds to the enzyme via tightly held bonds for example, Covalent bonds. So let's discuss in more detail what the difference between these Cofactors are. So metal ions include the minerals such as magnesium, zinc and potassium and so forth while our coenzymes are basically vitamin derivatives and this includes biotin and Thiamin Pyrophosphate. Now, biotin is basically a water soluble B vitamin. Specifically, it's the B seven vitamin while our Thiamine pyrophosphate is also a B vitamin derivative. Now, our coenzymes can be subdivided into these two groups but what exactly is the major difference between these two groups?