So this is around where the concentration inside our cells is usually at. So basically, equation three, this equation here, as we'll see in just a moment, can be used to give us insight into how enzymes operate under typical physiological conditions. That is, when the concentration of the substrate is much, much smaller than our Km value. So when we're right around here along that x axis, now, what can we assume when we basically state the following statement? So when the concentration of our substrate is much smaller than Km, this is the assumption that we can make. So let's take a look at the following equation.