So in diagram A, we take our solution of three proteins and we pour it into our funnel in diagram B. That protein mixture, at the initial moment that we pour it in, basically collects at the top of the column and it has not separated yet. So this is our protein mixture that consists of three proteins. Protein one, protein two and protein three. Now, after we wait some time, what happens is the gravitational force will basically pull on those molecules and the protein that has the least positive charge. So protein three with the negative charge will basically be pulled and it will repel those negatively charged beads.