Now, once the substrate molecule actually goes into this central cavity, that closes these two domains, and it also causes these two green domains the ATP binding cassettes to actually interact with one another. So it creates conformational changes in these two green structures and that increases their affinity for ATP molecules. And so in the next step, what happens is because we have these two cavities on the ATP binding consents that means two ATP molecules can actually fit into these two locations. And so two ATP molecules go into these two locations and once the ATP molecules move into that location that creates conformational changes in the entire structure of the transmembrane domain. And what happens is that induces this opening on the other side, the posing side of the membrane where that molecule actually came in. So it came in, let's say from the inside.