And so before we can replicate, we actually have to separate these two strands of DNA and we have to isolate this DNA template of interest. So we basically heat our solution that contains this plasma, and then we separate one of the strands of DNA. So this strand right over here, the inner one, which has the sequence tat, GCC, CTT, TGC, CCT, the same sequence that we discussed here. Now, in step two, we now create that DNA primer that contains that point mutation and we add it into our solution at the right temperature so that the kneeling process will take place. And so now the DNA primer will essentially hybridize with this section of that DNA template that we're going to use to basically synthesize our polynucleotide chain. And now we add our DNA polymerase along with the four types of building blocks, the four types of deoxy nucleus cytrophosphate molecules.