So normally, as we go from A to E, the number of bands should decrease. And what that means is we're getting rid of those unwanted proteins and we're focusing in on that protein that we actually want to study. And that is confirmed by these measurements because as we purify the sample, the specific activity has to increase and so should our purification level. And we also have to keep track of the yield. We don't want our yield to drop to a very small amount. For example, if following our procedure, this yield would have been, let's say 5%, then this procedure would not have been efficient because at the end, even though we would have had a high purification value, that yield is not enough.