So three is equal to three. So we can basically transform this equation to give us the maximum velocity, the maximum rate of that enzyme mixture simply by replacing the enzyme substrate concentration with the e total concentration. And this is given by this equation here. So once again, when all the active sides are filled, the reaction is said to be operating at a maximum velocity, at a maximum rate given by V max. And we can simply transform this equation into this equation by changing this concentration to the total enzyme concentration. And what this basically is telling us is all the active sites on all the enzymes are filled with that substrate molecule and therefore we are at a maximum operating rate.