And so what that will do is that will disrupt those electric interactions between protein One and these negatively charged beads. And so by pouring our solid solution, we're breaking the electric interactions between the protein and the negatively charged beads. And so now gravity can basically overcome that electric force, and this protein will begin to move down and eventually it will elude and we can collect it in another test tube. So let's suppose this is our test tube that contains that protein number one that we wanted to actually isolate in the first place. So we can see ion exchange chromatography is actually only helpful if there is a big difference between the net charges on our protein. We cannot use ion exchange chromatography to purify proteins.