Now, for every physical and for every biological reaction, these two thermodynamical laws must be obeyed. Now, we can even use these laws to explain many different reactions and phenomena that exist in biochemistry. For example, we can use the second law of thermodynamics to basically explain the hydrophobic effect. So let's suppose we have a beaker of pure water. So inside the beaker, all we have are these water molecules that are moving about and which are interacting with other water molecules via hydrogen bonds. Now, let's suppose we take two non polar molecules and place them into our beaker.