So this lost that H atom. And so we have a negative charge, because once that oxygen loses our H atom, it gains a negative charge for this, 110.8. So that means the nitrogen lost that H atom and it is neutralized, so it gains a charge. Or the nitrogen has a charge of zero, because one of the H atoms is lost. For Glutamate, we're once again above 4.1. So this will have a charge of negative one.