So in step number one, we want to increase the temperature of that solution in which this DNA molecule is found to about 95 degrees Celsius for about 15 seconds. And what this does is it breaks those electrostatic bonds, those hydrogen bonds that exist between those two strands of DNA. And what we have is we have a separation, we have the breaking of these bonds and so the separation of these two individual strands of DNA. So after step one, we now have this picture as shown in this diagram. So these two strands have now separated. Now once we separate the two strands, we begin decreasing the temperature of that solution.