And we also produce, once we cleave this section here we produce this fragment number four. And notice that because the protolytochemical leaves our disulfide bonds intact these red bonds aren't broken. And so this entire fragment number three actually consists of this green section that came from the green polypeptide and the blue section that came from that blue polypeptide. And we do this for a specific region because this is an important part of the process of diagonal electrophoresis as we'll see in just a moment. So these are basically our steps in diagonal electrophoresis. Now once we have these four fragments in our mixture in our solution we now place them onto a sheet and we run electrophoresis on that sheet along the horizontal direction.