Inside that beaker, we have our solution, the mixture of three different proteins. So we have the small green protein, intermediate red protein, and the large purple protein. So we pour it into the funnel, and the funnel essentially exits through this tiny little hole and ends up in the column. So what happens is, when we move on to step two, in step two, at the top of our column, we have this collection, the mixture of three different types of proteins. So initially, they have not yet separated because they have not yet traveled through the section of our column through the beads. Now, as we begin to wait, what happens is these proteins begin to travel.