Then this should connect. Valenine arginine glycine glycine. Then we have tryptophan and then Alanine and lysine So this is exactly what we mean by using the overlapping regions. After we expose our polypeptide to not just one proteolytic enzymes, but several proteolytic enzymes to basically determine what the order of our segments are. So step number one is we take the long polypeptide. We essentially cleave it with these different types of proteolytic enzymes and proteolytic molecules.