And that inhibits the activity of this enzyme. Now, once we actually bind the polyme molecule to the active side, that also that not only tells us where the active side is found, but it can also be used to basically study what kind of structural changes actually take place within the enzyme. As we'll see in just a moment. Now, one thing I'd like to point out about the active side of the enzyme, wherever that active side is, we have these three negative charges on this polymolecule. And the fact that we have three negative charges basically implies that inside the active side of this enzyme we'll find residues that contain positive charges. So basically, those positive charges in the residue interact with the negative charges of this bi substrate analog molecule, the poly molecule.