Now, enzymes are very sensitive and that basically means that the functionality and the rate of activity of enzymes does not only differ depend on non protein molecules such as Cofactors. The functionality and activity of enzymes also depends on the actual environment surrounding that protein molecule. Now, three major factors influence the enzymes activity and functionality and these are temperature, the acidity level, so the PH level, as well as the concentration of the substrate. So let's examine each one of these factors and see how they influence our proteins, our enzymes functionality. So let's begin with temperature. Now, increasing the temperature of the surroundings in which our protein enzyme is actually found in generally initially increases the rate or the activity of our enzyme.