We open up our knob, and so it enters test tube number two. And then when this reaches the bottom, once again, we turn a knob, open up this hole, and so this will elude into this test tube number three. And so now we have these three different separate test tubes that contain those isolated proteins, protein number one, protein number two, and protein number three. Now, I should emphasize that gel filtration chromatography only works if there is a relatively large difference in size between our proteins. If we use three proteins that are of the same size, this is not a very useful technique because these proteins will basically have the same exact rate of movement down our beats. And so we will not be able to separate them with this method.