Now, how exactly can we actually physiologically interpret the VMAX value? Well, the VMAX, the maximum rate of the enzyme, describes the highest number of substrate molecules that can be transformed into product molecules over a given time period when all the active sites are saturated are occupied with that substrate. That is the meaning of VMAX. Now, if we take the following equation and we solve for the rate constant of this reaction, K two, we get the following equation. So K two, the rate constant of this reaction here is equal to V max divided by E total. And this has an important physiological meaning.