Now, glutamate dehydrogenase is interesting because it not only uses NAD plus, but it can also use instead of the NAD plus, NADP plus. So we can replace this with NADP plus, but ultimately we use this or the enzyme uses this to basically remove the two electrons and the h to form the NADH. We also remove the h plus and we form a double bond between carbon and the nitrogen, and we form this shift based intermediate shown here. Now, in the second step, we actually have the diamondation step, or we can also see it as a hydrolysis step because we use a water to basically hydrolyze and remove that Ammonium. And so we replace the nitrogen, h two with an oxygen and we abstract that Ammonium. So we take the two HS from here and the two HS from the water, and we form that Ammonium.