So when we pour the crude mixture proteins, the protein with affinity for glucose will bind to the beads. For example, that protein could be glucose oxidase that we spoke about earlier, or it could be some other protein that also binds to glucose. So the other molecules, however, these proteins here did not bind to the glucose on the beads, and so they continue traveling to the bottom of that column. So let's take a look at the following five diagrams that basically describes how this process actually takes place. So here we have our experimental setup. We have our column, we have the funnel, and this is our beaker that contains that crude mixture of three proteins.