We get the following diagram. So, these blue beads are our negatively charged cellulose beads, as shown in the following diagram. Now, let's suppose we want to separate a mixture of three different proteins, and these proteins differ from one another based on their net charge. So one of these proteins we're going to call protein number one, has a net positive charge. So we have three of these charges, which make it very positive. The second protein, showed in a brown, contains two positive charges and one negative charge.