So once again, when all the active sides are filled, the reaction is said to be operating at a maximum velocity, at a maximum rate given by V max. And we can simply transform this equation into this equation by changing this concentration to the total enzyme concentration. And what this basically is telling us is all the active sites on all the enzymes are filled with that substrate molecule and therefore we are at a maximum operating rate. Now, how exactly can we actually physiologically interpret the VMAX value? Well, the VMAX, the maximum rate of the enzyme, describes the highest number of substrate molecules that can be transformed into product molecules over a given time period when all the active sites are saturated are occupied with that substrate. That is the meaning of VMAX.