And so the concentration is zero. And because no substrate is present in that beaker, no substrate will be bound onto the active side of the enzyme. And so the velocity, the rate of that enzyme will be zero. And so in trial one, point number one is zero. Now, in trial two, we then add a certain amount of concentration of S, let's say this many S molecules, and then we measure that corresponding y value, that velocity of that enzyme. And so suppose we get the following value.