And when the amide moves away, we basically make room in the active side for a water molecule to actually enter because remember, it's the water molecule that will ultimately also act as a nuclear phile to basically help hydrolyze that peptide bond. So in the next step, once the amide product departs, we have the water molecule that comes into place. And so this water molecule, it basically positions itself into the same position that we had this amide in this step. And once it positions into this location, the lone pair of electrons on the nitrogen of this side chain of the histidine basically interact with this age and they deprotonate that water molecule. Now, this is a very important step because just like serine contains the alcohol and the alcohol is not a strong enough nuclear file. So what happens is the H atom is removed to create alcoxide and transform into a good nucleophile.