And in addition, we remove this H to form the water molecule and form the double bond between this carbon and this carbon here. And once we form this double bond, this water molecule that comes in in step two will basically undergo a hydration reaction. The water will act as a nucleophile, and instead of attacking this carbon, it will attack this carbon. Because if the water molecule attacked this carbon, we would have simply reformed the citrate molecule. But if the water attacks this carbon, which is basically less hindered because it contains a smaller group on this side compared to this large group here, the water molecule is able to actually attack from this side because of less hindrance. And so once it attacks that side, we form the isocitrate molecule.