And so after the purification method, we have this molecule. And now we can place our cassette, that newly synthesized DNA molecule that was created in the laboratory. We can basically make it in such a way that the ends contain the sticky ends, complementary ends, which can stick to the end of this initial orange strand of DNA. And so now we mix it in the presence of once again, DNA lie gaze. And the liegase will basically combine the complementary sequences of these two ends to these two ends. And we form the following newly synthesized plasmid that contains this insertion, this cassette.