And the same thing is true for these other bands, as shown. Now, if we take this horizontal slap and we place it into our SDS Page setup, we can now separate our proteins based on size. So this is one direction, the horizontal direction of separation, and this is our vertical direction of separation. And that's why this is called a two dimensional gel electrophoresis process, because we separate along the x axis, then along the y axis, and those are two different dimensions. So once we take this and place it into our electrophoresis setup, we see that what begins to happen is they begin to migrate down towards the positively charged end of this SDS Page setup. And so if any one of these bands consists of different proteins that have different mass values, that are different sizes, we're going to be able to separate them along the vertical direction based on their size.