Now these parts are basically known as the flanking sequence. And the reason they're called flanking sequence is because they're found right next to the target sequence. And those primers are actually going to bind to these flanking sequences as we'll see in just a moment. So in step number one, we want to increase the temperature of that solution in which this DNA molecule is found to about 95 degrees Celsius for about 15 seconds. And what this does is it breaks those electrostatic bonds, those hydrogen bonds that exist between those two strands of DNA. And what we have is we have a separation, we have the breaking of these bonds and so the separation of these two individual strands of DNA.