And the largest proteins move the slowest because they have the greatest resistance. Now, once we form the setup, we also basically want to form the monoclonal antibody that is specific for that protein that we want to study. And that protein that we want to study, we call the antigen. So we synthesize this monoclonal antibody, we call the primary antibody that can bind to that antigen, the protein that we actually want to locate. Now, once we form the monoclonal antibody and once we run gelatrophrases, we then transfer that separated protein sample onto a polymer sheet. And this step is basically done to make it easier for us to work with our separated protein mixture.