And these positive charges come from six different amino acids which are found on the beta one and the beta two subunits. So let's take a look at the following diagram which we spoke about in the previous lecture. So this is our normal hemoglobin, and hemoglobin contains the beta one and the beta two subunits, as well as these two alpha subunits. Now, at the center of these individual subunits are the heme groups. And at the center of the actual hemoglobin molecule is this pocket of space. And in the pocket of space, we have three amino acids coming from the beta one subunit, we have histidine 141, Lysine 82, and HistoGene two.