So to see what we mean, let's take a look at the following diagram. So this is the eukaryotic mRNA molecule. So we initiate the process of protein synthesis when the methionine tRNA complex brings that methionine onto the aug, then we establish the reading frame. And then the ribosome essentially reads every codon one at a time in a sequential manner until we reach this stop codon, UAA. Now, once we reach UA, the release factors bind onto the UAA sequence and that causes the dissociation of this polypeptide chain. So this is the first amino acid.