So the isoelectric focusing technique is basically a method that we can use to purify and mixture proteins by using a specific property of the protein we call the isoelectric points. So let's take a look at the following diagram, which basically describes the setup of isoelectric focusing. So in the setup, we basically create a special type of gel, and we create a PH gradient along that gel. So let's suppose we take a gel. We take the gel, we place the gel into a special apparatus, and we create a PH gradient. What that means is on one side, let's say on the left side of our gel, we're going to have a low PH acidic environment.