And method number three, we're going to call transfection with calcium phosphate. Transfection simply means the introduction, the injection of that DNA molecule, the foreign DNA, into a eukaryotic cell without using any viral agent. That's what we mean by transfection. So let's begin with injection with the micro pipette. So we essentially have this very, very small instrument called a micro pipette that contains a very small diameter. And we can essentially hold the nucleus of the cell.