So it basically shifts the entire curve, the oxygen binding curve to the right side with respect to pure hemoglobin that does not contain the two three BPG. Now, how do we say that two three BPG binds onto hemoglobin? So what we said was at the center of the hemoglobin is this positively charged pocket, is this region of space that contains a positive charge or positive charges. And these positive charges come from six different amino acids which are found on the beta one and the beta two subunits. So let's take a look at the following diagram which we spoke about in the previous lecture. So this is our normal hemoglobin, and hemoglobin contains the beta one and the beta two subunits, as well as these two alpha subunits.