And so what happens is another enzyme known as DNA gyrase, which is a topo isomerase, basically creates negative super coils. It binds to our double stranded DNA and it induces negative super coils which removes the stress that are caused by the process of unwinding. So basically, DNA gyrase indirectly assists the process of DNA replication by removing the positive super coils that are formed by the process of unwinding. So in this diagram we have the DNA gyrase. So the DNA gyrase binds to our double strand DNA molecule and it creates the negative super coils which decrease the number of positive super coils in our double stranded DNA molecule. Now, the problem with separating our two single strand of DNA is the following once we separate our two single strands of DNA, they want to naturally and spontaneously reform those hydrogen bonds because by reforming the hydrogen bonds that stabilizes the double stranded DNA molecules.