So let's examine each one of these factors and see how they influence our proteins, our enzymes functionality. So let's begin with temperature. Now, increasing the temperature of the surroundings in which our protein enzyme is actually found in generally initially increases the rate or the activity of our enzyme. And this is primarily because by increasing the temperature we give the substrate more energy so that it has more energy to basically overcome that activation barrier. Now, the problem with increasing the temperature high or increasing the temperature continually is our proteins have tertiary structure. And remember that at a high enough temperature, the proteins tertiary structure can break down.