And at the conditions of our body temperature and neutral physiological PH, this reaction simply does not take place. So we conclude the following three facts about peptide bonds and the biosynthesis of peptide bonds. So peptide bonds form via a dehydrolysis reaction. And what that means is at the end of forming a single peptide bond we always release a water molecule. Now we can also say that the breaking of a peptide bond is a hydrolysis reaction. So we have to use a water molecule to actually break this peptide bond to give back this carbon its oxygen and this nitrogen its two H atoms.