We isolate it, we place it into our beaker that contains only this molecule here. And then we move on to step number two. And in step number two, we want to basically replicate this DNA molecule. And so what that means is we need three different things. We need a DNA primer, we need DNA polymerase and we need the building blocks, the four types of normal deoxy nucleotide triphosphate. So in step two, the solution containing the single strand of DNA, this one here is mixed with number one or a a labeled radioactively labeled DNA primer.