Another procedure, gel filtration Chromatography, in which we separate the proteins based on size. And so following that, we extract that sample, we place it into this weld, and this is the band that we get. And so this band disappears, other bands disappeared. So what that means is we're slowly purifying the protein where we're moving those unwanted proteins as shown by padded increase in the band distribution number. So that is what should be shown by this table. So let's calculate this box.