Well, usually the next process is to take that gene and produce a restriction map for that particular gene. Now what exactly is a restriction map? Well, let's suppose that we have the following ruler and this ruler describes as our gene of interest that we amplify. So this is the double stranded DNA molecule that describes the interest, that describes the gene that we're studying. So what a restriction map is? It's basically a description of all the different locations found on this gene where our restriction enzymes can bind to and cleave that gene.