But that protein, enzyme number one, that has the high affinity for glucose, will essentially bind onto that glucose molecule that is bound onto the beads. And this will happen ultimately at the beginning of our column. Now, some of these enzymes will make it farther down, but most of these enzymes bind in the beginning, towards the beginning of that column. So we have enzyme number one in this, and we have a bunch of enzymes number one throughout our beads, throughout the column. Now, enzyme two and three don't bind to any of the bees, and so they simply continue moving all the way to the bottom. So we see that the mixture of protein two and three will essentially be mixed together because none of them actually bind, none of them are attracted to those glucose molecules bound to our beets.