But when the denaturing agents were removed via the process of dialysis by using a semipermeable membrane, the enzyme eventually reformed its original tertiary structure. Now, what this basically shows is it gives us evidence that it's the primary sequence, it's the primary structure, it's that specific sequence of amino acids that essentially dictates the proper formation of the tertiary structure of that enzyme. Now let's move on to experiment two and see what experiment two was. In experiment two, he basically took that beaker that contained our denatured enzyme as well as the excess beta mercapto ethanol and our urea. And instead of removing these two agents at the same exact time, he first removed the beta mercapto ethanol. And then after some time, he removed that urea.