So we know that when calcium binds to things like proteins, it creates conformational changes in the proteins, and that exposes hydrophobic regions of the protein to the aqueous environment. And what that means is the calcium protein complexes, for the most part, become insoluble in that aqueous environment. And so what that can lead to is precipitation. So if there is a high concentration of calcium for a very long period of time in a cytoplasm, that can actually cause damage, that can actually lead to the precipitation of many different types of proteins within that side of plasma. So during steady state conditions inside the cell, there is a very low concentration of calcium, about 100, that's much, much smaller than the outside concentration. And this is because calcium ions can readily bind to the negatively charged regions of proteins forming insoluble complexes that can be harmful to the cell.