Now, if we take a look at the following diagram, we have three different curves. So the y axis is the rate of product formation for this enzyme catalyzed reaction and the x axis is the concentration of that substrate molecule. Now, at low concentration of the substrate molecule, we're going to exist predominantly in the T state. And it's this green curve that describes the Michaela's mental curve for this particular enzyme, for this particular coronary structure. And so notice at some particular substrate concentration the rate of activity will be low and that's because the active sites of the enzyme have a low affinity for that enzyme. But as we begin to increase the concentration of that substrate, that begins to shift the entire curve into the r state.