And by using different restriction enzymes, we can basically place insert that DNA fragment into different sections along our plasmid. So a DNA fragment of interest, the recombinant DNA for example, can be inserted into a plasmid by using restriction enzymes, we can use different restriction enzymes to insert the fragment at different locations along that plasmid. Now, if we use a restriction enzyme that cuts along a gene and we insert that DNA molecule into a gene in the middle of a gene, that will essentially inactivate that gene. And that gene will no longer be able to produce the protein that the gene actually encodes. And this process is known as insertional inactivation. So by inserting the fragment into a gene, we inactivate that gene.