Now let's move on to the mechanism of action. How exactly does this cleaving process take place? So restriction enzymes are these proteins found in these natural biological system cells. And they cleave via the process of hydrolysis phosphodiastor bonds that hold nucleotides together. And these restriction enzymes basically cleave two phosphodiastor bonds in a single double helix DNA molecule and they cleave at specific palindromic sequences. Now, to see what we mean by all that, let's take a look at a specific type of restriction enzyme that is found in E. Coli bacterial cells known as ECoR one.