So once again, what actually promotes what stimulates fatty acid synthesis? Well, when the level of ATP in the matrix is high, this means that we no longer need to actually synthesize any more ATP. And therefore the ATP will create a negative feedback loop that will inhibit isocitrate dehydrogenase. And this causes a build up, is citrate, which in turn causes a build up of citrate molecules. And once we actually form citrate, which involves transferring acetyl, the Cetil group from acetyl co enzyme onto oxalacetate, then that citrate can actually move across the inner and the outer membrane of the mitochondria and into the cytoplasm of that cell. So in step three, we have citrate is then transported across the membrane and into the cytoplasm of that cell.