So let's suppose we have a six amino acid polypeptide. So we have alanine, arginine, phenylalanine, glycine, aspartate, and glycine. Now, of course, usually you don't know what that protein actually is, but in this lecture, I give you the composition of that protein to basically show you how this method actually works. So once we have that beaker, and inside that beaker, we have the solution that consists of only this type of protein. What do we do first? Well, ultimately, what we want to do is we want to separate the amino acids, the different amino acids, into different compartments.