And as we go from one step to another step, if the procedure is working we have to get less bands and less bands and less bands until we get a single band as we'll see in just the moment. And that basically means we're purifying our sample because we're removing some of those proteins with specific mass values as we'll see in just a moment. And every time we conduct a procedure we also calculate these five quantities. And these five quantities basically tell us if our technique is actually working. So let's actually see by what we mean. So let's actually see what we mean by taking a look at the following diagrams.