These three codons essentially stimulate these release factors to bind onto that stop codon. And that essentially causes the dissociation of that ribosome complex. And that essentially concludes and terminates that synthesis of that particular polypeptide chain. So to see what we mean, let's take a look at the following diagram. So this is the eukaryotic mRNA molecule. So we initiate the process of protein synthesis when the methionine tRNA complex brings that methionine onto the aug, then we establish the reading frame.