And this is called insertional inactivation. That means that gene will not produce the corresponding protein. And to see what we mean and how this is useful, let's take a look at the following diagram. So let's take this PBR three, two, two plasmid. And as shown on the board, so we have this green gene that codes for that protein that gives the cell resistance to ampicillin. And we have this blue gene that gives the cell resistance to tetracycline.