Well, one way to break the bonds is to heat our solution that contains that double stranded DNA. So if we heat our solution with that double stranded DNA molecule of interest to a temperature of 95 degrees Celsius for about 15 seconds, then that's just enough time to basically break all those bonds between our two strands and separate our two strands. Now the next process is we want to actually anneal those DNA primers that we form. So in a laboratory, because we know what the flanking sequence is, we can basically build the DNA primers that will bind onto the three end of our DNA strands. So that is shown in the following diagram. So step two is annealing of the DNA primers, the heated DNA solution.