Now, the method that we're going to use is known as the Cassette Mutagenesis method. So a procedure called Cassette Mutagenesis can be used to insert specific segments of DNA into the plasmid known as cassettes. So we essentially take our plasma and as always, we mix it with our restriction enzymes. And these restriction enzymes basically cleave at position one and position two. So we basically produce these two fragments that differ by size. And because they differ by size, and because we want to remove this fragment here, we can use some type of purification method, for example, gel electrophoresis, to remove this molecule here.