So this entire molecule will rearrange to basically form this molecule here. And this is known as PTH amino acid, where in this particular case, because this amino acid is Alanine, then that means this is PTH Alamine. If this amino acid was something else, let's say Glycine, then this would be PTH, glycine and so forth. So in the second step, we were able to break that first peptide bond while keeping the other peptide bonds intact. And so now we have this labeled amino acid, the PTH Allamine, and this remaining polypeptide that now consists of five amino acids. And by using a chromatography technique and because this is labeled, we can separate this and then determine what this amino acid actually is.