Remember, annealing is the process by which two complementary sequences of nucleotides essentially hybridized. They bond, they form these hydrogen bonds. And so as long as we use the proper temperature conditions, this annealing process will still take place because all the other bases are paired up correctly. So, once again, the important step of this procedure is to create a short DNA primer that is complementary to the side to be altered. However, this primer should be changed in a way to make sure the protein will contain aspartate instead of glutamate. And notice that even though one of the bases are mismatched, the DNA primer will still uneal to that DNA molecule because all the other bases are paired up correctly, assuming we also use the correct temperature conditions.