So what we do now is we take these two different sources of DNA, we add a restriction enzyme, so we cut them at specific locations and then we add DNA ligase to basically produce this recombinant plasmid that contains this blue section that we basically want to amplify. So this is the same exact process that we followed right over here. And now we take this recombinant plasmid and we insert it into bacterial cell. And that bacterial cell will basically divide many, many times via binary fission and produce many identical copies of these recombinant plasmids. And ultimately we can take these bacterial cells and we can once again isolate these recombinant DNA plasmids. And now we can use the same restriction enzymes to basically cut at the same exact locations.