Then we follow that with the third cell that contains the third plasmid and the fourth cell that contains this fourth plasmid. And so at the end of the step, what we have is a colony that consists of millions of millions of these identical plasmids. And we have a colony for each and every one of these different types of cells. So once we form the colony of cells in each one of these petri dishes, so now we have four different petri dishes, we can basically lyse the cells and by lysing the cells, we basically release those plasmids. And now we can expose the plasmids to these same restriction enzymes. And what that does is it breaks down the restriction enzymes and it releases each one of these genes.