Now, what is the major difference between glucokinase and hexokinase? Well, there are two important differences. Number one, glucokinase has a much lower affinity for that glucose substrate molecule than hexokinase. In fact, hexokinase is 50 times more likely to bind to the glucose substrate than that glucokinase. And that will create a very important difference, as we'll see in just a moment. The second important difference between hexokinase and gluco kinase is that hexokinase is inhibited by glucose six phosphate when the concentration is high, but glucokinase is not effective, is not inhibited by that glucosex phosphate.