So there are three steps that we essentially have to follow. In step one, what we want to do is we want to take our quarterly structure and break it down. We want to denature our protein. We want to break down the non covalent interactions and the disulfide bonds that exist between those two polypeptide chains. So we need to expose the quadrinary structure to a denaturing solution. For example, we can use urea or guanidine hydrochloride to basically break down those non covalent interactions holding our two polypeptide chains.