And this basically describes that situation when all the active sites in our enzyme mixture are filled with the substrate. This is when the velocity, the rate of the enzyme mixture will be at a maximum. So the enzyme operates at a maximum rate when all the active sites are filled. And this is given by this equation. Now, if we take a look at the following curve when exactly under what conditions will the blue curve actually reach the maximum velocity? Well, notice as we increase the concentration, this blue curve essentially reaches or approaches that maximum velocity asymptotically.