So we form this intramolecular ester molecule known as six phosphorluquino delta lactone. Now let's move on to step two. So in step two, what we want to basically do is we want to prepare the molecule for a decorboxylation reaction. And the way that we prepare the molecule is by opening up the ring structure. We open up that ring structure via a hydrolysis reaction which is catalyzed by an enzyme known as Latinase. So Latinase catalyzes the hydrolysis of this aster bond.