And now the DNA polymerase will bind onto the primer and begin synthesizing in the five to three direction from this end to this end. So it will move all the way along this DNA template. And we'll use that DNA template to synthesize that new strand of DNA that now contains this codon sequence GAC that codes for glutamate. So now notice we have this DNA molecule where one of the strands is this DNA template strand and the other strand is the newly synthesized green strand that contains this, this slightly modified codon sequence. Now, if we take this plasmid and repeat step one and two, so essentially we heated, we separated, then we basically isolate that green DNA molecule that was synthesized in step two. And now we add that DNA polymerase as well as the four types of deoxy nucleuside triphosphates.