This is called the positively charged amino acid species. So at a PH, at a very acidic PH of around one, this amino acid, and in general, amino acids, exist in their positively charged species form. Now, as we begin to increase our PH, we decrease the concentration of the H plus ions inside our solution. And at around two, this H found on the carboxylic acid group begins to dissociate. It deprotonates. It loses that H atom, and so it gains that full negative charge.