Now, when we begin to increase the substrate concentration inside the cells of our body, the substrate molecules will begin to bind onto the active side. So let's suppose one substrate molecule binds onto one of the active sides of this enzyme. And once the binding takes place, what that does is it shifts the equilibrium of this entire equation slightly to the right side, from the T state to that r state. And so as we increase the concentration of the substrate, even more of those substrate molecules begin to bind onto the active sites. And eventually, that shifts the entire curve to the right side. And at a very high substrate concentration inside our cells, the entire curve will be shifted.