So in the first step after step one, we basically take our test tube with our homogeneous. We place it into the baranas and it basically rotates and eventually it separates that mixture. So the very dense material ends up at the bottom. So this section that is known as the pellet and the rest ends up at the surface. So higher up this entire section that is known as the supernatural. Now in the first centrifugation process, our acceleration is 1000 g's, where g is simply the gravitational pull due to the earth 9.8 meters/second squared.