So the lower temperature will allow for the hybridization between the primers and the DNA and one DNA primer anneals to the three end of each one of these DNA strands. The reason we have the Anneal at the three end? Because the DNA polymerase, as we'll see in step three, always reads that DNA molecule beginning at the three end and moving towards the five end because it synthesizes from the five end to the three N. So this green primer is the primer that Anneales to the three end of this trend. And the blue DNA primer is the primer that anneales to the three end of the other DNA molecule as shown in that diagram. And finally in step three, what we have to do is we have to use a special DNA polymerase that is heat resistant and we can obtain this DNA polymerase from prokaryotic cells that live in hot springs. Because in hot springs the temperature is high, it's about in the.