So Lysine contains PKA of 10.8 on that side chain group. And so we're at five, which is below 10.8. So this will have a positive charge on that nitrogen. Let's move on to the next one. So we're at Glutamate. For Glutamate, the PK is 4.1, and since five is above 4.1, that means this oxygen will have lost that H atom and so it will have a negative charge.