So just like the enzyme E One contains this sulfidel group in the active side, enzyme Two also contains a cysteine residue that contains that sulfhydrol group. And so we simply have a shutter link, a transferring of the Ubiquitin from enzyme One to enzyme two. So we see that the activated version of Ubiquitin that we formed in step number one is now transferred onto the second enzyme by attaching it onto the cysteine residue of enzyme Two, E Two. Now, in the final step, this is basically what happens. So we have the final enzyme, E Three, which is known as the Ubiquitin protein ligase, basically enters the picture. And now what it does is it transfers the Ubiquitin group onto this protein target here.