And because we're talking about E. Coli cell, let's suppose we're going to use a restriction enzyme found in E. Coli cells known as ECoR one. Now, ECoR one will essentially cut our DNA of the alpha phase at two locations somewhere here and here. And we produce the following three fragments. So fragment one and fragment three, the side fragments, are also known as the arms. And this is the center of the middle, fragment number two. Now, what we can do is we can essentially separate these DNA molecules and then we can remove the fragment number two.