This separates the proteins based on their isoelectric point. So the PH value at which the net charge on the protein is zero. So if two or more proteins, however, have the same pi value, they will be found on the same exact band in this diagram. So we have 12345 of these different bands. And what each of these bands basically means, we have either a single or many proteins within each one of these bands. For example, let's suppose we're looking at this band.