And what that means is at the end of forming a single peptide bond we always release a water molecule. Now we can also say that the breaking of a peptide bond is a hydrolysis reaction. So we have to use a water molecule to actually break this peptide bond to give back this carbon its oxygen and this nitrogen its two H atoms. Now fact number two is peptide bond formation. The biosynthesis of peptide bonds is a thermodynamically unfavorable process. And what that means is we always have to input energy.