Basically, this molecule acts as a coenzyme and helps gap dehydrogenates to transform the gap molecule glyceroaldehyde three phosphate into one three bisphospho bisphosphoglycerate. And in this process, we see that NAD plus was used up and it was never actually regenerated in the remaining steps of glycolysis. And so this means that glycolysis basically depends on NAD plus. And when the concentration of NAD plus basically runs out, the process of glycolysis will come to a stop and the cell will no longer be able to actually produce those ATP molecules. And the way that our cells fix the problem is by taking the Peruvate molecule and actually using that Peruvate molecule, metabolizing it to regenerate those NAD plus molecules so in order for Glycolysis to proceed over and over and over, the NAD plus must be regenerated. And so the cell regenerates the NAD plus by breaking down, metabolizing those pyruvate molecules produced at the end of Glycolysis.