And this describes the blue curve describes a cordon point of about 0.1. There's a difference of 22% according to this graph. And that means 22% of the hemoglobin will unload the oxygen in size. This situation when we have a higher percentage of two three BPG, a higher concentration of two three BPG inside our blood. So how will increasing the amount of two three BPG affect the oxygen binding curve? Well, it will shift the curve to the right, as seen in the following diagram.