And as the H plus ions flow this way into the mitochondrial matrix, the ATP synthase also basically combines ADP adenosine diphosphate and a phosphate group to synthesize our ATP molecules. So basically, for a single NADH that is formed in either the citric acid cycle or pyruvate decarboxylation, one NADH produces three ATP molecules. However, if we examine the NADH molecule that is formed in glycolysis to actually get from the cytoplasm where glycolysis takes place and into the mitochondrial matrix, the NADH molecule has to transport via our mitochondrial inner and outer membrane and that requires energy. So for our NADH to get from the cytoplasm to the mitochondrial matrix we have to use a single ATP molecule. And that means the net result. So the net production of our NADH molecule that comes from glycolysis is not three, but only two ATP molecules because we had to use that one ATP molecule when we went from the cytoplasm into the mitochondrial matrix.