### Question
How is the "biological monitoring" defined?


### Answer
The term **biological monitoring** is defined in *33 U.S.C. § 1362* as follows -

> the determination of the effects on aquatic life, including accumulation of pollutants in tissue, in receiving waters due to the discharge of pollutants (A) by techniques and procedures, including sampling of organisms representative of appropriate levels of the food chain appropriate to the volume and the physical, chemical, and biological characteristics of the effluent, and (B) at appropriate frequencies and locations.

#### Citation:
  - *33 U.S.C. § 1362*
  - Heading: **CHAPTER 26-WATER POLLUTION PREVENTION AND CONTROL > SUBCHAPTER V-GENERAL PROVISIONS**

