Definition: In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.
Input: S2 Contents S1. General information …S3 Plasmid construction …S3 Preparation of mRNA and 70S initiation complex for rapid kinetics …S6 Supplementary References. …S7 S3 Escherichia coli strain DH5α was used to amplify plasmid DNA. Oligonucleotides were purchased from Invitrogen. The compounds NCTn (n = 5, 6, 7, and 8) used in this study were synthesized as previously reported (1). Chemicals were from Roche, Sigma, or Merck. Radioactive compounds were from Hartmann Analytic. Ribosomes from E. coli MRE 600, fMet-tRNA fMet , Leu-tRNA Leu , EF-Tu, EF-G, and initiation factors were prepared as described (2-5). tRNA Gly , tRNA Lys , tRNA Val were prepared from total E. coli tRNA by consecutive column chromatographies on Sepharose 4B (GE Healthcare), Phenyl Sepharose (GE Healthcare), and DEAE Toyopearl (Tosoh Bioscience). Aminoacylated tRNAs were purified by reversed-phase HPLC on an RP-18 HPLC column (250 mm × 10.5 mm, Merck) equilibrated with buffer (20 mM ammonium acetate, pH 5.0, 10 mM magnesium acetate, 400 mM NaCl) using a gradient of 0%-15%) ethanol. Aa-tRNAs were precipitated with ethanol and dissolved in water; concentrations were determined photometrically by absorbance at 260 nm.

Small synthetic molecule as an activator for −1 ribosomal frameshifting S2