Definition: In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.
Input: Edwardsiella tarda is a major pathogen in aquatic environments that can cause heavy economic losses. An improved method for quick and accurate detection of E . tarda by loop-mediated isothermal amplifi cation (LAMP) with two additional loop primers was developed by targeting the EsrB gene ( EsrB -LAMP). In this method, the Mg 2+ concentration, reaction temperature, and reaction time were optimized to 8 mmol/L, 61°C, and 40 min, respectively. The detection limit with the EsrB gene was as low as 10 copies, which is 100 times more sensitive than that of conventional polymerase chain reaction (PCR). The EsrB -LAMP assay was shown more sensitive and rapid than previously reported LAMP assays targeting the hemolysin gene ( hemolysin -LAMP) for detection of E . tarda . The EsrB -LAMP was also highly specifi c to E . tarda and had no cross-reaction with 13 other strains of bacteria. The assay can be carried out in a simple heating device and the EsrB -LAMP products can be visually detected by adding fl uorescent dye to the reaction mixture. Taken together, the improved EsrB -LAMP diagnostic protocol has the potential for detection of E . tarda from indoor and outdoor samples.

An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplifi cation by targeting the EsrB gene*