Definition: In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.
Input: INTRODUCTION HB assay and construction of plasmids: HeLa cells were transfected with appropriate plasmids, pretreated with different concentrations of HB (Sigma), and labeled with [ 35 S] methionine/cysteine (Amersham). Cells were incubated in the presence or absence of HB, harvested and lysed. Total proteins were immunoprecipitated with appropriate antibodies, and analyzed by SDS 15% polyacrylamide gel electrophoresis. Plasmids pFlagE and pFlagN was constructed by cloning an EcoRV-and EcoRIdigested PCR fragments into EcoRV-and EcoRI-digested pFlag vector. The Flag-tag was fused to the N-terminal end of the E protein. Mutations were introduced into the E gene by two rounds of PCR and confirmed by automated sequencing. The mutant constructs included in this study are summarized in Fig. 1.  RESULTS Alteration of Membrane Permeability by the Expression of E Protein

VIROPORIN ACTIVITY OF SARS-CoV E PROTEIN