Definition: In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.
Input: Antibody-based therapeutics is attracting more attention in the post-genome era, in contrast to a diminution in the initial high expectation for rapid development of gene-based therapeutic modalities. In support to the antibody-based therapeutics, the advent of recent technologies has made human antibody screening and production progressively more economic. Among those technologies, phage-display antibody library has been successfully applied in the antibody-based drug development both as fully human antibody sources and tools for antibody engineering. Building up a high-quality antibody library with a large library size and high diversity has been crucial for successful isolation of antibodies. Here we describe an efficient strategy for the construction of a large naïve phage-display human Fab library with one-step cloning. Optimization of each key step is extensively discussed and simplified protocols for library panning and Fab production are also described. Phage display; Fab; antibody library; IgM; one-step cloning Building a high-quality phage-displayed antibody library requires a source of large number of well-diversified antibody genes. A variety of immunoglobulin gene sources including blood lymphocytes, bone marrow, and spleen have been used for library construction. From these sources IgM-derived antibody genes have been successfully used for library construction and selection of high-affinity antibodies (4-7), even though IgM-specific antibodies are typically of relatively low affinity in vivo compared to antibodies from the

Construction of a Large Naïve Human Phage-Displayed Fab Library Through One-Step Cloning