Definition: In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.
Input: A facile one-pot two-enzyme chemoenzymatic approach has been established for gram (Neu4,5Ac 2 α3Lac, 1.33 g) and preparative-scale (Neu4,5Ac 2 α3LNnT) synthesis of monotreme milk oligosaccharides. Other O-acetyl-5-N-acetylneuraminic acid (Neu4,5Ac 2 )-or 4-O-acetyl-5-N-glycolylneuraminic acid (Neu4Ac5Gc)-containing α2-3-sialosides have also been synthesized in preparative scale. Used as an effective probe, Neu4,5Ac 2 α3GalβpNP was found to be a suitable substrate by human influenza A viruses but not bacterial sialidases. One-pot multienzyme (OPME) synthesis: Monotreme milk oligosaccharides and other sialosides containing a 4-O-acetyl-sialic acid were synthesized in gram (1.33 g for Neu4,5Ac 2 α3Lac) or preparative (10-56 mg) scales using a one-pot two-enzyme (OP2E) sialylation system containing a bacterial cytidine 5′-monophosphate (CMP)-sialic acid synthetase and Pasteurella multocida sialyltransferase 3 (PmST3). Neu4,5Ac 2 α3Galβ4GlcNAcβ3Galβ4GlcβProN 3 (34)-20 mg, yield 81%; white solid. 1 H NMR (800 MHz, D 2 O) δ 4.93 (m, 1H), 4.67 (d, J = 8.0 Hz, 1H), 4.54 (d, J = 8.0 Hz, 1H), 4.47 (d, J = 8.0 Hz, 1H), 4.41 (d, J = 8.0 Hz, 1H), 4.14 (d, J = 3.2 Hz, 1H), 4.11 (dd, J = 9.6 and 3.2 Hz, 1H), 4.07 (t, J = 10.4 Hz, 1H), 3.98-3.54 (m, 28H), 3.44 (t, J = 6.4 Yu et al.

Effective one-pot multienzyme (OPME) synthesis of monotreme milk oligosaccharides and other sialosides containing a 4-O- acetyl sialic acid † HHS Public Access Author manuscript