 [ Symptoms ] does it make a difference how long it is been since last ejaculation does sperm have to be kept fresh to be fertile [ Disease ] hi ok as sperm grows inside your testes it just keeps moving through and the old sperm just enters your body its like an on going thing but masturbating once a week does not hurt this helps us men to keep things moving as you seem to be worried about your sperm freshness as does sex on a regular basis say twice a week do hope this helps you good luck