 [ Symptoms ] does it make a difference how long it is been since last ejaculation does sperm have to be kept fresh to be fertile [ Disease ] hello hope this answer finds you all health and happy further i do like to share that it is really very important for the sperm to be fresh for fertility as having sex or by other way keeping your sperm fresh this helps in keeping sperms clear from the testicles and the natural built up dna damage dna damage lessened in the sperm of men who had daily sex old sperm was cleared and replaced by fresh cells thanks