Ilona Wilmont, Mark Loeffen, Thomas Hoogeboom
A qualitative study on the facilitators and barriers to adopting the N-of-1 trial methodology as part of clinical practice: potential versus implementation challenges
International journal of qualitative studies on health and well-being
native prokaryotic promoters share common sequence patterns species dependent understudied species limited data challenging predict strength existing promoters generate novel promoters developed promogen collection nucleotide language models generate speciesspecific functional promoters across dozens species data parameter efficient way twentyseven speciesspecific models collection finetuned pretrained model trained multispecies promoters systematically compared native promoters escherichia coli bacillus subtilisspecific artificial promogengenerated promoters pgps demonstrated hold distribution patterns native promoters regression model developed score generated either promogen another competitive neural network overall score pgps higher encouraged silico analysis experimentally characterized twentytwo b subtilis pgps results showed four tested pgps reached strong promoter level active furthermore developed userfriendly website generate speciesspecific promoters 27 different species promogen work presented efficient deeplearning strategy de novo speciesspecific promoter generation even limited datasets providing valuable promoter toolboxes especially metabolic engineering understudied microorganisms