Marina Serdiouk, Philip Rodkin, Rebecca Madill, Handrea Logis, Scott Gest
Rejection and victimization among elementary school children: the buffering role of classroom-level predictors
Journal of abnormal child psychology
tumor mutational burden tmb significant biomarker predicting immune checkpoint inhibitor response clinical performance wholeexome sequencing wesbased tmb estimation received less attention compared panelbased methods study aimed assess reliability comparability wesbased tmb analysis among laboratories routine testing conditions multicenter study conducted involving 24 laboratories china using silico reference data sets accuracy comparability tmb estimation evaluated using matched tumornormal data sets factors accuracy variant calls limit detection lod wes test size regions interest rois used tmb calculation tmb cutoff points analyzed laboratories consistently underestimated expected tmb scores matched tumornormal samples 50 falling within 30 tmb interval samples low tmb score 25 received consensus interpretation accuracy variant calls lod wes test roi tmb cutoff points important factors causing interlaboratory deviations study highlights realworld challenges wesbased tmb analysis need improved optimized research aid selection reasonable analytical procedures minimize potential methodologic biases estimating tmb clinical exome sequencing tests harmonizing tmb estimation clinical testing conditions crucial accurately evaluating patients response immunotherapy