Robert Rus, Philipp Scheider, Sebastian Farr
Early Distal Radius Remodeling after Radius Dome Osteotomy in Madelung's Deformity
Journal of wrist surgery
criminal investigations particularly sexual assaults frequently require identification body fluid type addition body fluid donor provide context cases achieved conventional methods however certain scenarios alternative molecular methods required example detection menstrual fluid vaginal material able identified using conventional techniques endpoint reversetranscription pcr rtpcr currently used purpose amplify body fluid specific messenger rna mrna transcripts forensic casework realtime quantitative reversetranscription pcr rtqpcr similar method utilises fluorescent markers generate quantitative results form threshold cycle cq values despite uncertainty surrounding body fluid identification interpretation guidelines utilise categorical statements probabilistic modelling realistic reflects biological variation well known performance method research describes application various machine learning models singlesource mrna profiles obtained rtqpcr assesses performance multinomial logistic regression mlr nave bayes nb linear discriminant analysis lda used discriminate following body fluid categories saliva circulatory blood menstrual fluid vaginal material semen identified performance mlr somewhat improved quantitative dataset original cq values used overall accuracy approximately 095 rather presenceabsence coded data overall accuracy approximately 094 indicates quantitative information obtained rtqpcr amplification useful assigning body fluid class three classification methods mlr performed best utilised receiver operating characteristic curves observe performance body fluid class clear methods found difficulty classifying menstrual blood samples future work involve modelling body fluid mixtures common samples analysed part sexual assault investigations