Brian M Doornenbal, Tim van Zutphen, Lise F E Beumeler, Rimke C Vos, Mark Derks, Hinke Haisma, M Elske van den Akker-van Marle, Jessica C Kiefte-de Jong
Development and validation of a Context-sensitive Positive Health Questionnaire (CPHQ): A factor analysis and multivariate regression study
Journal of patient-reported outcomes
2omethylation nm one abundant modifications found mrnas noncoding rnas contributes many biological processes normal functioning trna protection mrna degradation decapping exoribonuclease dxo protein biogenesis specificity rrna recent advancements singlemolecule sequencing techniques long read rna sequencing data offered oxford nanopore technologies enabled direct detection rna modifications sequencing data study propose biocomputational framework nmnano predicting presence nm sites direct rna sequencing data generated two human cell lines nmnano framework integrates two supervised machine learning ml models predicting nm sites extreme gradient boosting xgboost random forest rf kmer embedding evaluation benchmark datasets direct rna sequecing hela hek293 cell lines demonstrates high accuracy 99 xgboost 92 rf identifying nm sites deploying nmnano hela hek293 cell lines reveals genes frequently modified nm hela cell lines 125 genes identified frequently nmmodified showing enrichment 30 ontologies related immune response cellular processes hek293 cell lines 61 genes identified frequently nmmodified enrichment processes like glycolysis protein localization findings underscore diverse regulatory roles nm modifications metabolic pathways protein degradation cellular processes source code nmnano freely accessed httpsgithubcomjangalabnmnano