Belayneh Shetie Workneh, Enyew Getaneh Mekonen, Mohammed Seid Ali
Determinants of knowledge, attitude, and practice towards first aid among kindergarten and elementary school teachers in Gondar city, Northwest Ethiopia
BMC emergency medicine
primary human hepatocytes used facets liver research vitro studies xenobiotic disposition toxicity clinical management liver failure unfortunately cellular stress isolation cryopreservation causes highly unpredictable loss ability attach form cellmatrix cellcell interactions reasoning problem could mitigated postthawing stage applied labelfree quantitative global proteomics analyze differences attached nonattached fractions cryopreserved human hepatocyte batches hepatocytes unable attach collagen matrix showed many signs cellular stress including glycolytic phenotype activation heat shock response ultimately leading apoptosis activation analysis activated stress pathways revealed increase early apoptosis immediately postthawing suggested possibility stress reversal therefore transiently treated cells compounds aimed decreasing cellular stress via different mechanisms brief exposure pancaspase apoptosis inhibitor zvadfmk restored attachment ability promoted differentiated morphology well formation 3d spheroids zvadfmk treatment restored metabolic transport functions maintained sensitivity hepatotoxic insults altogether study shows differentiation function suboptimal human hepatocytes restored cryopreservation thus markedly increasing availability precious cells