Alasdair Kennedy, Ukwamma Abosi, Clare Gilbert, Jalikatu Mustapha
Factors associated with adherence to treatment in patients with open angle glaucoma in Sierra Leone, West Africa: patient demographics and questionnaire
International ophthalmology
oxygen exchange reactions occurring catalytic sites fof1atp synthasef1atpase imprint unique record molecular events catalytic cycle atp synthesishydrolysis work presents new theory oxygen exchange tests oxygen exchange data recorded atp hydrolysis mitochondrial f1atpase mf1 apparent rate constant oxygen exchange governing intermediate pihoh exchange accompanying atp hydrolysis determined kinetic analysis 50000fold range substrate atp concentration 015000 corresponding 200fold range reaction velocity 35650 moles pimoles f1atpase1 s1 isotopomer distributions 18opi species containing 0 1 2 3 labeled oxygen atoms predicted theory quantified shown perfect agreement experimental distributions entire range medium atp concentrations without employing adjustable parameters novel molecular mechanism steadystate multisite atp hydrolysis f1atpase proposed results show steadystate atp hydrolysis f1atpase occurs three sites occupied mgnucleotide various implications arising models energy coupling atp synthesishydrolysis atp synthasef1atpase discussed current models atp hydrolysis f1atpase including postulated singlemolecule data shown effectively bisite models contradict data trisite catalysis formulated naths torsional mechanism energy transduction atp synthesishydrolysis since first appearance 25 years ago shown better accord experimental record total biochemical information atp hydrolysis integrated consistent model torsional mechanism atp synthesishydrolysis shown elucidate elementary chemical mechanical events within black box enzyme catalysis energy metabolism f1atpase