Ipek Gonullu, Alper Bayazit, Sengul Erden
Exploring medical students' perceptions of individual and group-based clinical reasoning with virtual patients: a qualitative study
BMC medical education
neglected tropical diseases especially caused helminths constitute common infections worlds poorest people amongst schistosomiasis bilharzia snail fever caused blood flukes genus schistosoma ranks second malaria terms human impact two hundred million people infected close 800 million risk infection drug screening helminths poses unique challenges parasite cannot cloned difficult target using gene knockouts rnai consequently lead identification validation involve phenotypic screening parasites exposed compounds whose effects determined analysis ensuing phenotypic responses efficacy leads thus identified derives one even unknown molecular mechanisms action two immediate significant challenges confront stateoftheart area development automated quantitative phenotypic screening techniques mapping quantitative characterization totality phenotypic responses parasite paper investigate propose solutions latter problem terms following 1 mathematical formulation algorithms allow rigorous representation phenotypic response space parasite 2 application graphtheoretic network analysis techniques quantitative modeling characterization phenotypic space 3 application aforementioned methodology analyze phenotypic space mansoni one etiological agents schistosomiasis induced compounds target pololike kinase 1 plk 1 gene recently validated drug target approach first bioimage analysis algorithms used quantify phenotypic responses different drugs next responses linearly mapped low dimensional space using principle component analysis pca phenotype space modeled using neighborhood graphs used represent similarity amongst phenotypes graphs characterized explored using network analysis algorithms present number results related nature phenotypic space mansoni parasite well algorithmic issues encountered constructing analyzing phenotypicresponse space particular phenotype distribution parasite found distinct shape topology also quantitatively characterized phenotypic space varying critical model parameters finally maps phenotype space allows visualization reasoning complex relationships putative drugs systemwide effects serve highly efficient paradigm assimilating unifying information phenotypic screens lead identification lead optimization