Stephanie J. Salyer|Thomas R. Gillespie|Innocent B. Rwego|Colin A. Chapman|Tony L. Goldberg
Epidemiology and Molecular Relationships of Cryptosporidium spp. in People, Primates, and Livestock from Western Uganda
University of Wisconsin–Madison|Emory University|Makerere University|McGill University|Makerere University|Makerere University|University of Wisconsin–Madison
cryptosporidium one common parasitic diarrheal agents world known zoonosis studied cryptosporidium people livestock nonhuman primates region kibale national park uganda land use change near park resulted fragmented forest patches containing small remnant populations wild primates interact intensively local people livestock goal investigate risk factors cryptosporidium infection assess crossspecies transmission using molecular methodsdiagnostic pcr revealed prevalence cryptosporidium 324 humans 111 nonhuman primates 22 livestock case humans residence one particular community associated increased risk infection fetching water open water source although 485 infected people reported gastrointestinal symptoms frequency significantly different people tested negative 447 cryptosporidium coinfection giardia duodenalis associated increased reporting gastrointestinal symptoms fecal consistency different infected versus uninfected people animals dna sequences cryptosporidium oocyst wall protein gene placed infections within wellsupported c parvumc hominis clade however two sequences recovered primates core parks protected area fell divergent subclade identical published sequences c parvum c hominis c cuniculus suggesting possibility separate sylvatic transmission cyclecryptosporidium may transmitted frequently among species western uganda people livestock wildlife interact intensively result anthropogenic changes forests parasite may undergo hostspecific transmission interactions occur parasite appear strong effects human animal health perhaps persistent lowlevel shedding immunity