Cathrine Hoyo|Amy P. Murtha|Joellen M. Schildkraut|Randy L. Jirtle|Wendy Demark?Wahnefried|Michele R. Forman|Edwin S. Iversen|Joanne Kurtzberg|Francine Overcash|Zhiqing Huang|Susan K. Murphy
Methylation variation at<i>IGF2</i>differentially methylated regions and maternal folic acid use before and during pregnancy
Duke University Hospital|Duke Medical Center
folic acid fa supplementation pregnancy associated decreased risk neural tube defects although recent reports suggest may also increase risk chronic diseases evaluated exposure maternal fa supplementation pregnancy relation aberrant dna methylation two differentially methylated regions dmrs regulating insulinlike growth factor 2 igf2 expression infants aberrant methylation regions associated igf2 deregulation increased susceptibility several chronic diseases using selfadministered questionnaire assessed fa intake pregnancy 438 pregnant women pyrosequencing used measure methylation two igf2 dmrs umbilical cord blood leukocytes mixed models used determine relationships maternal fa supplementation pregnancy dna methylation levels birth average methylation h19 dmr 612 compared infants born women reporting fa intake pregnancy methylation levels h19 dmr decreased increasing fa intake 28 p003 49 p004 intake pregnancy respectively methylation decrease pronounced male infants p001 methylation alterations h19 dmr likely important mechanism fa risks andor benefits conferred utero stable methylation marks dmrs regulating imprinted genes acquired gastrulation may serve archives early exposures potential improve understanding developmental origins adult disease