Patricia A Iglesias, Melanie Revilla, Birgit Heppt, Anna Volodina, Clemens Lechner
Protocol for a web survey experiment studying the feasibility of asking respondents to capture and submit photos of the books they have at home and the resulting data quality
Open research Europe
alzheimers disease ad common form dementia older adults damages brain results impaired memory thinking behaviour identification differentially expressed genes related pathways among affected brain regions provide information mechanisms ad past decade several studies reported many genes associated ad wealth information become difficult follow interpret results conflicting case worth integrated study multiple datasets helps increase total number samples statistical power detecting biomarkers study present integrated analysis five different brain region datasets introduce new genes warrant investigation aim study apply novel combinatorial optimisation based metaanalysis approach identify differentially expressed genes associated ad across brain regions study microarray gene expression data 161 samples 74 nondemented controls 87 ad entorhinal cortex ec hippocampus hip middle temporal gyrus mtg posterior cingulate cortex pc superior frontal gyrus sfg visual cortex vcx brain regions integrated analysed using method results compared two popular metaanalysis methods rankprod genemeta obtained analysing individual datasets find genes related ad consistent existing studies new candidate genes previously related ad study confirms upregualtion infar2 ptma along regulation gphn rab2a psmd14 fgf novel genes psmb2 wnk1 rpl15 sema4c rwdd2a large found differentially expressed across brain regions investigation genes may provide new insights development ad addition identified presence 23 noncoding features including four mirna precursors mir7 mir570 mir1229 mir6821 dysregulated across brain regions furthermore compared results two popular metaanalysis methods rankprod genemeta validate findings performed sensitivity analysis removing one dataset time assess robustness results new findings may provide new insights disease mechanisms thus make significant contribution near future towards understanding prevention cure ad