Kerry A Ramsbottom, Ananth Prakash, Yasset Perez-Riverol, Oscar Martin Camacho, Zhi Sun, Deepti J Kundu, Emily Bowler-Barnett, Maria Martin, Jun Fan, Dmytro Chebotarov, Kenneth L McNally, Eric W Deutsch, Juan Antonio Vizcaíno, Andrew R Jones
Meta-Analysis of Rice Phosphoproteomics Data to Understand Variation in Cell Signaling Across the Rice Pan-Genome
Journal of proteome research
coursebased undergraduate research experiences cures effective means transforming learning teaching science involving students scientific process potential importance microbiome shaping environmental health disease makes investigations microbiomes excellent teaching tool undergraduate microbiology present cure based microbiome bean beetle callosobruchus maculatus model system undergraduate laboratory education despite extensive research literature bean beetles little known microbiome making ideal system discoverybased cure cure students acquire microbiological technical skills characterizing culturable unculturable members beetle gutmicrobial community students plate beetle gut homogenates different media describe colonies formed estimate taxonomic diversity extract dna colonies interest pcr amplify the16s rrna gene sanger sequencing use ncbinblast database taxonomically classify sequences additionally students extract total dna beetle gut homogenates highthroughput pairedend sequencing perform bioinformatic statistical analyses bacterial communities using combination openaccess data processing software activity allows students engage studies microbiomes realworld context apply concepts laboratory techniques investigate either student facultydriven research questions gain valuable experiences working large highthroughput datasets cure designed implemented either 6weeks half semester 12weeks full semester allowing flexibility within curriculum furthermore studentgenerated data cure including bacterial colony phenotypic data fulllength 16s rrna gene sequences cultured isolates bacterial community sequences gut homogenates compiled continuously curated openaccess database bean beetle microbiome project website facilitating generation broader research questions across laboratory classrooms