Lukáš Čunek, Jan Ondřej, Ivana Blažíčková, Veronika Pupíková, David Lacko, Tomáš Prošek, Katarína Šafárová
Handwriting Quality: Psychometric Properties of Two Evaluation Scales With a Czech Sample
The American journal of occupational therapy : official publication of the American Occupational Therapy Association
platelets small circulating fragments cells play important roles thrombosis haemostasis immune response inflammation cancer growth although anucleate contain rich rna repertoire offers opportunity characterise changes platelet gene expression health disease whilst achieved conventional rna sequencing large input highquality rna hence blood volume required unless preamplification step added along specialist bioinformatic skills data analysis interpretation developed transcriptomics nextgeneration sequencingbased approach overcomes limitations termed plateletseq method requires low levels rna input require specialist bioinformatic analytical skills describe methodology sample collection processing data analysis specifically blood samples stored 8 days 4 c prior analysis platelets isolated using multistep centrifugation purity 1 leucocyte per 026x106 platelets optimal gene expression analysis applied plateletseq normal adult blood samples show ageassociated variations minor genderassociated differences contrast platelets patients myeloproliferative neoplasms show differences platelet transcript profiles normal disease subtypes illustrates potential applicability plateletseq biomarker discovery studying platelet biology patient samples also opens avenues assessing platelet quality fields transfusion research