Han Zhang, Shigang Ge, Mohd Rashid Bin Mohd Saad
Formative assessment in K-12 English as a foreign language education: A systematic review
Heliyon
advances singlecell level analytical techniques especially cytometric approaches led profound innovation biomedical research particularly field clinical immunology resulted expansion highdimensional data posing great challenges comprehensive unbiased analysis conventional manual analysis thus becoming untenable handle challenges furthermore newly developed computational methods lack flexibility interoperability hampering accessibility usability adapted seurat r package originally developed singlecell rna sequencing scrnaseq analysis highdimensional flow cytometric data analysis based 20marker antibody panel analyses tcell profiles adult blood cord blood cb showcased robust capacity seurat flow cytometric data analysis validated spectre another highdimensional cytometric data analysis package conventional manual analysis importantly identified unique cd8 tcell population defined cd8cd45racd27cd161 cell predominantly present cb characterised ifnproducing potential cytotoxic properties using flow cytometry experiments scrnaseq analysis published dataset collectively identified unique human cb cd8cd45racd27cd161 tcell subset demonstrated seurat widely used package scrnaseq analysis possesses great potential repurposed cytometric data analysis facilitates unbiased thorough interpretation complicated highdimensional data using single analytical pipeline opens novel avenue datadriven investigation clinical immunology