Haijing Ma, Shelley D Golden
Impact of New York City Cigarette Floor Price Policy on Reducing Smoking Disparities
Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco
human microbiome exists throughout body essential maintaining various physiological processes including immunity dysbiotic events associated autoimmunity peptidylarginine deiminase pad enzymes citrullinate selfproteins related rheumatoid arthritis ra induce production anticitrullinated protein antibodies acpas lead inflammation joint damage present investigation carried demonstrate expression homologs pads arginine deiminases ads citrullinated proteins members human microbiota achieve objective used 17 microbial strains specific polyclonal antibodies pabs synthetic peptide derived residues 100200 human pad2 antipad2 pab recombinant fragment amino acids 326 611 human pad4 antipad4 pab human anticitrulline pab affinity acpas ra patient western blot wb enzymelinked immunosorbent assay elisa elution test griess reagent used crosssectional casecontrol study patients diagnosed ra control subjects inferential statistics applied using nonparametric kruskalwallis test mannwhitney u test generated spss program members phyla firmicutes proteobacteria harbor homologs padsads citrullinated antigens reactive acpas ra patients microbial citrullinome homolog enzymes padsads extensive human microbiome involved production acpas findings suggest molecular link microorganisms dysbiotic microbiota ra pathogenesis