Rita Mojtahedzadeh, Shirin Hasanvand, Aeen Mohammadi, Sahar Malmir, Mehdi Vatankhah
Students' experience of interpersonal interactions quality in e-Learning: A qualitative research
PloS one
mammalian cells contain two types mannose 6phosphate man6p receptors mprs 300 kda cationindependent ci mpr 46 kda cationdependent cd mpr two mprs overlapping function intracellular targeting newly synthesized lysosomal proteins required efficient targeting despite extensive investigation relative roles specialized functions mpr targeting specific proteins remain questions fundamental interest one possibility man6p glycoproteins transported mprs may subsets preferentially transported investigate conducted proteomics analysis serum mice lacking either mpr reasoning lysosomal proteins selectively transported given mpr preferentially secreted bloodstream absence purified identified man6p glycoproteins glycopeptides wildtype cdmprdeficient cimprdeficient mouse serum found lysosomal proteins proteins currently thought lysosomal function different mass spectrometric approaches spectral count analysis nanospray lcmsms experiments unlabeled samples lcmalditoftof experiments itraqlabeled samples revealed number proteins appear specifically elevated serum mprdeficient mouse man6p glycoforms cellular repressor e1astimulated genes 1 tripeptidyl peptidase heparanase elevated absence cdmpr man6p glycoforms alphamannosidase b1 cathepsin prosaposin elevated absence cimpr results confirmed western blot analyses select proteins study provides comparison different quantitative mass spectrometric approaches provides first report proteins whose cellular targeting appears mprselective physiological conditions