Abubakr A A Al-Shoaibi, Puja Iyra, Julia H Raney, Kyle T Ganson, Erin E Dooley, Alexander Testa, Dylan B Jackson, Kelley P Gabriel, Fiona C Baker, Jason M Nagata
Associations Between Adverse Childhood Experiences and Early Adolescent Physical Activity in the United States
Academic pediatrics
carotid atherosclerosis cas complication atherosclerosis panoptosome inflammatory programmed cell death pathway event regulated panoptosome complex cass panoptosomerelated genes porgs yet studied hence screening panoptosomerelated diagnostic genes treating cas vital introduced transcriptome data screen differentially expressed genes degs cas subsequently wgcna analysis utilized mine module genes panoptosis score performed differential expression analysis cas samples vs standard samples obtain casrelated differentially expressed genes singlecell level venn diagram executed identify panoptosomerelated differential genes pordegs associated cas lasso regression rf algorithm implemented executed build diagnostic model additionally performed immune infiltration gene set enrichment analysis gsea based diagnostic genes verified accuracy model genes singlecell nuclear sequencing rtqpcr validation clinical samples well vitro cellular experiments identified 785 degs associated cas 4296 module genes panoptosis score obtained obtained 7365 1631 casrelated degs singlecell level respectively 67 pordegs retained venn diagram subsequently 4 panoptosomerelated diagnostic genes cntn4 filip1 phgdh tfpi2 identified via machine learning cellular function tests four genes showed genes essential roles maintaining arterial cell viability resisting cellular senescence obtained four panoptosisrelated diagnostic genes cntn4 filip1 phgdh tfpi2 associated cas laying theoretical foundation treating cas