Levi Ross, Michael A Preston, Torhonda C Lee, Jala Lockhart, Jordan Young, Angela Wood, Ralph Wood
Exploring Information Seeking Anxiety Among Localized Prostate Cancer Patients
American journal of men's health
antibodies essential research tools whose performance directly impacts research conclusions reproducibility owing central role alzheimers disease dementias hundreds distinct antibody clones developed microtubuleassociated protein tau multiple proteoforms despite breadth offer limited understanding performance poor antibody selectivity hindered research progress validate large panel tau antibodies western blot 79 reagents immunohistochemistry 35 reagents address reagents ability detect target proteoform selectivity impact protein phosphorylation antibody binding performance human brain samples antibodies detected tau high levels many failed detect lower endogenous levels wb nonselective binding proteins affected half antibodies tested several crossreacting related map2 protein whereas oligomeric tau t22 antibody reacted monomeric tau wb thus calling question specificity tau oligomers despite presumption total tau antibodies agnostic posttranslational modifications found phosphorylation partially inhibits binding many antibodies including popular tau5 clone combine highsensitivity reagents massspectrometry proteomics cdna sequencing demonstrate presumptive tau knockout human cells continue express residual protein arising exon skipping providing evidence previously unappreciated gene plasticity finally probing human brain samples large panel antibodies revealed presence ctermtruncated versions main tau brain isoforms control tauopathy donors ultimately identify validated panel tau antibodies employed western blotting andor immunohistochemistry reliably detect even low levels tau expression high selectivity work represents extensive resource enable reinterpretation published data improve reproducibility tau research overall accelerate scientific progress