Ng'wamba Sitta Ngissa, Erica Sanga, Mussa Kelvin Nsanya, Belinda Kweka, Evangelista Malindisa, Rosemarie Mwaipopo
Curriculum-based sexual and reproductive health education: revealing its relevance for risky sexual behaviors among secondary school students in Mwanza, Tanzania
Reproductive health
micrornas mirnas indispensable regulation skeletal muscle performed rna sequencing rnaseq establish comprehensive mirna profiling goats seven stages namely 45 f45 65 f65 90 f90 120 f120 135day f135 fetuses newborn b1 90dayold b90 kids total 421 known mirnas 228 goat novel mirnas identified data average abundance 19 mirnas seven stages exceeds 10000 reads per million furthermore 420 differentially expressed mirnas demirnas identified comparison group seven stages 80 uniquely differentially expressed b1 b90 comparison groups pathway analysis indicated group associated release muscle hypertrophy regulation myoblast proliferation besides 305 demirnas clustered three significantly enriched profiles profiles 11 16 19 function analysis revealed profile 16 related muscle hypertrophy differentiation profile 11 involved multiple enzyme activities metabolic processes muscle cells profile 19 involved material transport structural stability two highly expressed mirnas three key mirnas chimir3283p chimir767 chimir150 profiles verified consistent data quantitative realtime pcr results provided catalog goat muscleassociated mirnas allowing us better understand transformation mirna roles mammalian muscle development