Anna M. Hoy|Rachel J. Lundie|Alasdair Ivens|Juan F. Quintana|Norman Nausch|Thorsten Forster|Frances M. Jones|Narcis B. Kabatereine|David W. Dunne|Francisca Mutapi|Andrew S. MacDonald|Amy H. Buck
Parasite-Derived MicroRNAs in Host Serum As Novel Biomarkers of Helminth Infection
Centre for Immunity, Infection and Evolution|University of Edinburgh|Walter and Eliza Hall Institute of Medical Research|Centre for Immunity, Infection and Evolution|University of Edinburgh|Centre for Immunity, Infection and Evolution|University of Edinburgh|Centre for Immunity, Infection and Evolution|University of Edinburgh|University of Edinburgh|University of Cambridge|Ministry of Health|University of Cambridge|Centre for Immunity, Infection and Evolution|University of Edinburgh|Centre for Immunity, Infection and Evolution|University of Edinburgh|Centre for Inflammation Research|University of Manchester|University of Edinburgh|Centre for Immunity, Infection and Evolution
micrornas mirnas class short noncoding rna play important roles disease processes animals present highly stable cellfree form body fluids examine capacity host parasite mirnas serve tissue serum biomarkers schistosoma mansoni infectionwe used exiqon mirna microarrays profile mirna expression livers mice infected mansoni 7 weeks postinfection thirtythree mouse mirnas differentially expressed infected compared nave mice 2 fold change p005 including mir199a3p mir199a5p mir214 mir21 previously associated liver fibrosis settings five mouse mirnas also significantly elevated serum twelve weeks postinfection sequencing small rnas serum confirmed presence mirnas revealed eleven parasitederived mirnas detectable eight weeks post infection analysis host parasite mirna abundance qrtpcr extended serum patients low high infection sites zimbabwe uganda hostderived mirnas failed distinguish uninfected infected individuals however analysis three parasitederived mirnas mir277 mir34793p bantam could detect infected individuals low high infection intensity sites specificitysensitivity values 8980 8090 respectivelythis work identifies parasitederived mirnas novel markers mansoni infection mice humans potential used existing techniques improve mansoni diagnosis contrast although host mirnas differentially expressed liver infection abundance levels serum variable human patients may useful cases extreme pathology likely hold limited value detecting prevalence infection