Mohammad Abbasian Josheghani, Zahra Tagharrobi, Khadijeh Sharifi, Zahra Sooki, Fatemeh Zare Joshaghani, Mohammad Zare
The effects of auriculotherapy on depression among elderly people: A randomized controlled clinical trial
Complementary therapies in medicine
dairy goat good model production transgenic proteins milk using somatic cell nuclear transfer scnt however animals produced scnt often associated lung deficiencies recently produced six transgenic cloned dairy goats harboring human lactoferrin gene including three live transgenic clones three deceased transgenic clones died respiratory failure perinatal period imprinted genes important regulators lung growth may subjected faulty reprogramming present study first microsatellite analysis pcr dna sequence identification conducted confirm three dead kids genetically identical transgenic donor cells second cpg island methylation profile imprinted insulinlike growth factor receptor igf2r gene assessed lungs three dead transgenic kids normally produced kids using bisulfite sequencing pcr addition relative mrna level igf2r also determined realtime pcr results showed igf2r gene lungs dead cloned kids showed abnormal hypermethylation higher mrna expression levels control indicating aberrant dna methylation reprogramming one important factors death transgenic cloned animals