Elizabeth J. Corwin|Carol J. Hogue|Bradley D. Pearce|Cherie C. Hill|Timothy D. Read|Jennifer G. Mullé|Anne L. Dunlop
Protocol for the Emory University African American Vaginal, Oral, and Gut Microbiome in Pregnancy Cohort Study
Emory University|Emory University|Emory University|Emory University|Emory University|Emory University|Emory University
adverse birth neonatal outcomes disproportionately affect african american women infants compared racesethnicities significant research sought identify underlying factors contributing disparities current understanding remains limited constraining prevention early diagnosis treatment development next generation sequencing techniques contribution vaginal microbiome adverse maternal neonatal outcomes come consideration however microbiome pregnancy studies include african american women consider potential contribution nonvaginal microbiome sites consider effects sociodemographic behavioral factors microbiome conceived ongoing 5year longitudinal study biobehavioral determinants microbiome preterm birth black women intrarace study enable investigation risk protective factors within disparate group aim recruit 500 pregnant african american women enrolling study 814 weeks pregnancy participants asked complete questionnaires provide oral vaginal gut microbiome samples enrollment 2430 weeks chart review used identify pregnancy outcomes infections treatments complications dna extracted microbiome samples sequencing v3 v4 regions 16s rrna gene conducted processing mapping completed qiime operational taxonomic units otus mapped greengenes version 138 community state types csts diversity measures site time identified considered light demographic psychosocial clinical biobehavioral variables rich data set allow future consideration risk protective factors within groups women providing opportunity uncover roots persistent health disparity experienced african american families