David A Cook, Lee P Skrupky
Validation of the motivated strategies for learning questionnaire and instructional materials motivation survey
Medical teacher
gut microbiota recognized regulator brain development behavioral outcomes childhood nonetheless associations gut microbiota behavior often inconsistent among studies humans perhaps many hostmicrobe relationships vary widely individuals study aims stratify children based gut microbiota composition ie clusters identify novel gut microbiome clusterspecific associations stool metabolomic pathways child behavioral outcomes stool samples collected community sample 248 typically developing children 35 years gut microbiota analyzed using 16s sequencing lcmsms used untargeted metabolomics parentreported behavioral outcomes ie adaptive skills internalizing externalizing behavioral symptoms developmental social disorders assessed using behavior assessment system children basc2 children grouped based gut microbiota composition using dirichlet multinomial method differences metabolome behavioral outcomes investigated four different gut microbiota clusters identified cluster enriched bacteroides bifidobacterium ba2 distinct stool metabolome cluster characterized high bifidobacterium abundance bif well cluster ba2 associated lower adaptive skill scores subcomponent social skills cluster ba2 also significantly lower stool histidine urocanate turnover turn associated lower social skill scores clusterdependent manner finally cluster ba2 increased levels compounds involved galactose metabolism ie stachyose raffinose alphadglucose alphadglucose associated adaptive skill subcomponent daily living scores ie ability perform basic everyday tasks clusterdependent manner data show novel associations gut microbiota metabolites behavioral outcomes typically developing preschoolaged children results support concept clusterbased groupings could used develop personalized interventions support child behavioral outcomes video abstract