Bula Kere Oda, Ermias Lulekal, Bikila Warkineh, Zemede Asfaw, Asfaw Debella
Ethnoveterinary medicinal plants and their utilization by indigenous and local communities of Dugda District, Central Rift Valley, Ethiopia
Journal of ethnobiology and ethnomedicine
universal extensively adopted technique enzymelinked immunosorbent assay elisa used detect quantify small molecules many applications clinical analytical however generally students experiment mechanically using commercial elisa kits according instructions eventually produce standard curve calculate concentration sample measured cannot understand critical factors process method establishment study systematically introduced undergraduates using pathogenspecific antigen establishing indirect elisa method detect diagnostic target pathogen burkholderia pseudomallei course aimed develop experimental skills students improve scientific research knowledge fully embody organic combination scientific research teaching students independently selected diagnostic antigen target interest obtained antigen proteins using genetic engineering techniques established elisa method series conditional optimization experiments addition typical studentgenerated data experimental methods student feedback interpretation presented study overall students able combine abstract knowledge practice understand principles applications antigenantibody interactions thus enabling gain practical experience molecular biology techniques learn use principle establish elisa method detecting infectious diseases