Hae-Sook Hong, Jeong-Eun Park, Wan-Ju Park
[Predictors of hospitalization for alcohol use disorder in Korean men]
Journal of Korean Academy of Nursing
singlecell proteomics offers unparalleled insights cellular diversity molecular mechanisms enabling deeper understanding complex biological processes individual cell level develop integrated sample processing activematrix digital microfluidic chip singlecell proteomics amdmfscp employing amdmfscp approach dataindependent acquisition dia identify average 2258 protein groups single hela cells within 15 min liquid chromatography gradient performed comparative analyses three tumor cell lines hela a549 hepg2 machine learning utilized identify unique features cell lines applying amdmfscp characterize proteomes thirdgeneration egfr inhibitor ask120067resistant cells 67r parental ncih1975 cells observed potential correlation elevated vim expression 67r resistance consistent findings bulk sample analyses results suggest amdmfscp automated robust sensitive platform singlecell proteomics demonstrate potential providing valuable insights cellular mechanisms