Gemma Rodríguez, Nora Pérez, Gemma Núñez, Josep-E Baños, Mar Carrió
Developing creative and research skills through an open and interprofessional inquiry-based learning course
BMC medical education
species sex confirmation biological specimen play crucial role crime investigation however specimen found scene always trace quantity hard analyzed current methods moreover timeconsuming dna extraction sophisticated apparatus complex data processing make difficult satisfy demand speediness convenience pointofcare tests study first exhibit phosphatebased visual system fieldbased species sex identification derived trace bloodstain introducing phosphate ionbased colorimetry loopmediated isothermal amplification lamp result interpretation bloodstain directly submitted mitochondrial variant amplification owing enhanced amplification efficiency pyrophosphate ion hydrolyzation also colorimetric signal recognized naked eye result output within 30 min molybdophosphate generation aerosol contamination major conflict lamp solved integrating uracildna glycosylase system still holds constant temperature demonstration cytochrome b ychromosomal amelogenin employed identify species sex respectively achieved highly sensitive specific distinguishability strong interferential background accurate results obtained simulative degraded dated specimen indicates novel system may serve promising tool forensic practice