Ryan C Costantino, Eulho Jung, Michael Soh, Anita Samuel
Exploring Knowledge, Skills, and Abilities Needed for Army Pharmacist Readiness Using the Nominal Group Technique
Military medicine
morphogenetic programs coordinate cell signaling mechanical interactions shape organs systems synthetic biology key challenge determining optimal cellular interactions predicting organ shape size function physicsbased models defining subcellular force distribution facilitate challenging calibrate parameters models data solve inverse problem created bayesian optimization framework determine optimal cellular force distribution predicted organ shapes match experimentally observed organ shapes integrative framework employs gaussian process regression nonparametric kernelbased probabilistic machine learning modeling paradigm learn mapping functions relating morphogenetic programs maintain final organ shape calibrated tested method drosophila wing imaginal discs study mechanisms regulate epithelial processes ranging development cancer parameter estimation framework successfully infers underlying changes core parameters needed match simulation data imaging data wing discs perturbed collagenase computational pipeline identifies distinct parameter sets mimicking wildtype shapes enables global sensitivity analysis support regulation actomyosin contractility basal ecm stiffness generate maintain curved shape wing imaginal disc optimization framework combined experimental imaging identified piezo mechanosensitive ion channel impacts fold formation regulating apicalbasal balance actomyosin contractility elasticity ecm workflow extensible toward reverseengineering morphogenesis across organ systems realtime control complex multicellular systems