Taya A Collyer
The eye of the beholder: how do public health researchers interpret regression coefficients? A qualitative study
BMC public health
rapid advancements technology refine understanding intricate biological processes crucial emphasis remains understanding assumptions sources uncertainty underlying biological measurements particularly critical cell signaling research quantitative understanding fundamental mechanisms governing transient events essential drug development given importance homeostatic pathogenic processes western blotting technique developed decades ago remains indispensable tool investigating cell signaling protein expression proteinprotein interactions improvements statistical analysis methodology reporting undoubtedly enhanced data quality understanding underlying assumptions limitations visual inspection western blotting provide valuable additional information evaluating experimental conclusions using example agonistinduced receptor posttranslational modification highlight theoretical experimental assumptions associated western blotting demonstrate raw blot data offer clues experimental variability may fully captured statistical analyses reported methodologies article intended comprehensive technical review western blotting instead leverage illustrative example demonstrate assumptions experimental design data normalization revealed within raw data subsequently influence data interpretation