Jung-Min Kwak, Ju-Hee Kim
Psychometric Properties of the Korean Version of the Environmental Health Literacy Scale
International journal of environmental research and public health
abnormal immune system activation inflammation crucial causing parkinsons disease however still dont fully understand certain immunerelated genes contribute diseases development progression study aims screen key immunerelated gene parkinsons disease based weighted gene coexpression network analysis wgcna machine learning study downloaded gene chip data gene expression omnibus geo database used wgcna screen important gene modules related parkinsons disease genes important modules exported venn diagram important parkinsons diseaserelated genes immunerelated genes drawn screen immune related genes parkinsons disease gene ontology go analysis kyoto encyclopedia genes genomes kegg used analyze functions immunerelated genes signaling pathways involved immune cell infiltration analysis performed using cibersort package r language using bioinformatics method 3 machine learning methods least absolute shrinkage selection operator lasso regression random forest rf support vector machine svm immunerelated genes parkinsons disease screened venn diagram differentially expressed genes screened using 4 methods drawn intersection gene hub nodes hub gene downstream proteins parkinsons disease hub gene identified string database proteinprotein interaction network diagram drawn total 218 immune genes related parkinsons disease identified including 45 upregulated genes 50 downregulated genes enrichment analysis showed 218 genes mainly enriched immune system response foreign substances viral infection pathways results immune infiltration analysis showed infiltration percentages cd4 cells nk cells cd8 cells b cells higher samples parkinsons disease patients resting nk cells resting cd4 cells significantly infiltrated samples parkinsons disease patients ank1 screened hub gene analysis proteinprotein interaction network showed ank1 translated expressed 11 proteins mainly participated functions signal transduction iron homeostasis regulation immune system activation study identifies parkinsons disease immunerelated key gene ank1 via wgcna machine learning methods suggesting potential candidate therapeutic target parkinsons disease