Schahryar Kananian, Ahlke Kip, Hannah Schumm, Julia Giesebrecht, Anica Nicolai, Carmen Schade-Brittinger, Jens-Peter Reese, Cornelia Weise, Ricarda Mewes, Nexhmedin Morina, Thomas Ehring, Ulrich Stangier
Culturally adapted cognitive behavioural group therapy for mental disorders in refugees plus problem solving training (ReTreat): study protocol for a multicentre randomised controlled trial
BMJ open
lower respiratory tract infections lrtis represent globally prevalent detrimental diseases metagenomic nextgeneration sequencing mngs technology effectively addressed requirement diagnosis clinical infectious diseases study aimed identifying classifying opportunistic pathogens respiratory tractcolonizing microflora lrti patients using data acquired mngs analyses retrospective study performed employing mngs data pertaining respiratory samples derived 394 lrtis patients linear discriminant analysis effect size lefse analysis conducted discern discriminant bacteria receiver operating characteristic curves roc established demonstrate discriminant bacterial behavior distinguish colonization infection total 443 discriminant bacteria identified segregated three cohorts contingent upon correlation profiles detection frequency relative abundance order distinguish pathogens colonizing microflora among 119 emerging opportunistic pathogens cohort 2 occupied average area curve auc 0976 exhibiting prominent predictability distinguishing colonization infection 39 colonizing bacteria cohort 1 0961 285 rare opportunistic pathogens cohort 3 0887 ltris patients appeared modular form cohorts depicting complex microbial cooccurrence networks reduced diversity high degree antagonistic interactions respiratory tract microbiome study findings indicate therapeutic interventions target interaction networks rather individual microbes providing innovative perspective comprehending combating respiratory infections conclusively study reports profile lrtisassociated bacterial colonization opportunistic pathogens relatively largescale cohort might serve reference panel interpretation mngs results clinical practice