Migdalisel Colon-Berlingeri, Patricia A Burrowes
Teaching biology through statistics: application of statistical methods in genetics and zoology courses
CBE life sciences education
recently increasing number studies investigated mechanism action lactobacilli treatment nonalcoholic fatty liver disease nafld using four computational tools normfinder genorm delta ct bestkeeper six potential reference genes rg analyzed human liver cell line hepg2 cultivated 24 hours presence two strains heatkilled lactobacilli limosilactobacillus reuteri e lactiplantibacillus plantarum kg4 respectively different cultivation media dmem high glucose hg rpmi analysis revealed suitability rg similar two lactobacilli quite different two media commonly used rgs 18s rrna gapdh unstable dmem hg normalization mrna expression target gene encoding sterol regulatory elementbinding protein srebp1c different rgs resulted different expression profiles demonstrates validation candidate rgs specific experimental conditions crucial correct interpretation qpcr data addition choice media profound impact effect lactobacilli lipogenesis gene expression level shown transcription factor srebp1c