Theresa N Wang, Jennifer M Underhill, Savannah K Renshaw, Kelly R Haisley
Optimal Timing of Fundamentals of Endoscopic Surgery (FES) Testing in General Surgery Residency: Early Is Better
Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract
macroscopic dynamic models fermentation processes elementary modes em derived metabolic networks often used describe reaction stoichiometry simplified manner build predictive models parameterizing kinetic rate equations em procedure selection set em key step followed estimation reaction rates associated confidence bounds paper present method computation reaction rates cellular reactions em well algorithm selection em process modeling method based dynamic metabolic flux analysis dmfa proposed leighty antoniewicz 2011 metab eng 136 745755 additional constraints regularization analysis uncertainty instead using estimated uptake secretion rates concentration measurements used directly avoid amplification measurement errors numerical differentiation shown regularized dmfa em method significantly robust measurement noise methods using estimated rates confidence intervals estimated reaction rates obtained bootstrapping selection set em given st oichiometric model dmfa em method combined multiobjective genetic algorithm method applied real data cho fedbatch process measurements six fedbatch experiments 10 em identified smallest subset em based upon data described sufficiently accurately dynamic model estimated em reaction rates confidence intervals different process conditions provide useful information kinetic modeling subsequent process optimization