Lalita Fernandes, Anthony Menezes Mesquita
Developing knowledge and clinical competency in a respiratory system-based practice of final-year medical students through a novel structured bedside teaching module
Journal of education and health promotion
present study examined hypothesis changes oxidationreduction state thiol residues functional proteins play major role expression ventilatory responses conscious rats occur hypoxichypercapnic hh gas challenge upon return room air hh gas challenge vehicletreated rats elicited robust sustained increases minute volume via increases frequency breathing tidal volume peak inspiratory expiratory flows inspiratory expiratory drives minimally affecting noneupneic breathing index nebi hhinduced increases parameters except frequency breathing substantially diminished rats pretreated potent lipophilic disulfidereducing agent lddithiothreitol 100 molkg iv ventilatory responses occurred upon return room air also substantially different dithiothreitoltreated rats contrast pretreatment substantially higher dose 500 molkg iv lipophilic congener monosulfide nacetyllcysteine methyl ester lnacme minimally affected expression abovementioned ventilatory responses occurred hh gas challenge upon return room air effectiveness dithiothreitol suggests oxidation thiol residues occurs exposure hh gas challenge process plays essential role allowing expression posthh excitatory phase breathing however interpretation contradicted lack effects lnacme apparent conundrum may explained disulfide structure affording unique functional properties dithiothreitol comparison monosulfides specifically disulfide structure may give dithiothreitol ability alter conformational state functional proteins transferring electrons also possible dithiothreitol simply efficient reducing agent following systemic injection although one interpretation data effects dithiothreitol due reducing ability