Hersh Attal, Zhilian Huang, Win Sen Kuan, Yanyi Weng, Hann Yee Tan, Eillyne Seow, Li Lee Peng, Hoon Chin Lim, Angela Chow
N-of-1 Trials of Antimicrobial Stewardship Interventions to Optimize Antibiotic Prescribing for Upper Respiratory Tract Infection in Emergency Departments: Protocol for a Quasi-Experimental Study
JMIR research protocols
assessment platelet function key diagnosing bleeding disorders evaluating antiplatelet drug efficacy however prevailing onesizefitsall approach interpretation measures platelet reactivity arbitrary cutoffs often derived healthy volunteer responses aim compare wellused platelet reactivity assays blood plateletrich plasma obtained framingham heart study n 3429 assayed using range agonists 5 platelet assays light transmission aggregometry optimul aggregometry multiplate impedance aggregometry roche diagnostics total thrombusformation analysis system flow cytometry using linear mixedeffect models determined contribution preanalytical technical factors modulated platelet reactivity traits strong intraassay correlation platelet traits seen assays particularly multiplate velocity r 0740 ristocetin vs arachidonic acid contrast moderate interassay correlations observed r 0375 adenosine diphosphate optimul emax vs light transmission aggregometry large area curve expected antiplatelet drugs strongly reduced platelet responses aspirin use primarily targeting arachidonic acidinduced aggregation explained substantial variance 1735 p 459 10780 variance proportion 462 p2y12 antagonists blocking adenosine diphosphate responses 1612 p 675 1027 variance proportion 21 notably female sex older age associated enhanced platelet reactivity fasting status deviations standard venipuncture practices alter platelet reactivity significantly finally agonist batch phlebotomist assay technician assays require additional sample manipulation moderate large effect measured platelet reactivity caution must exercised extrapolating findings assays use standard ranges must medicationspecific sexspecific minimum researchers also consider preanalytical technical variables designing experiments interpreting platelet reactivity measures