Wendy Burton, Louise Padgett, Nicola Nixon, Louisa Ells, Kevin J Drew, Tamara Brown, Chirag Bakhai, Duncan Radley, Catherine Homer, Jordan Marwood, Pooja Dhir, Maria Bryant
Transferability of the NHS low-calorie diet programme: A qualitative exploration of factors influencing the programme's transfer ahead of wide-scale adoption
Diabetic medicine : a journal of the British Diabetic Association
thermal stability proteins primary metric evaluating physical properties although researchers attempted predict using machine learning frameworks performance dependent quality quantity published data due technical limitation thermodynamic characterization protein denaturation fluorescence calorimetry highthroughput manner challenging obtaining melting curve derives solely target protein requires laborious purification making far practical prepare hundred samples single workflow aimed overcome throughput limitation leveraging high protein secretion efficacy brevibacillus consecutive treatment platescale purification methodologies handling entire process expression purification analysis perplate basis enabled direct observation protein denaturation 384 samples within 4 days demonstrate practical application system conducted comprehensive analysis 186 single mutants singlechain variable fragment nivolumab harvesting melting temperature tm ranging 93 108c compared wildtype sequence findings allow datadriven stabilization protein design streamlining rational approaches