Md Dilsad Ahmed, Zuhair A Al Salim
Provision of Quality Physical Education to enhance the motive of Physical Activity and its underlying Behavior among university students
Heliyon
bladder cancer bca among prevalent malignant tumors affecting urinary system due highly recurrent nature standard treatments surgery often fail significantly improve patient prognosis research aims predict prognosis identify precise therapeutic targets novel treatment interventions collected screened genes related tgf signaling pathway performed unsupervised clustering analysis tcgablca samples based genes analysis revealed two novel subtypes bladder cancer completely different biological characteristics including immune microenvironment drug sensitivity using machine learning classifiers identified smad6 hub gene contributing differences investigated role smad6 bladder cancer singlecell transcriptome data additionally analyzed relationship smad6 immune checkpoint genes finally performed series vitro assays verify function smad6 bladder cancer cell lines revealed two novel subtypes bladder cancer among c1 exhibits worse prognosis lower drug sensitivity complex tumor microenvironment colder immune microenvironment compared c2 identified smad6 key gene responsible differences explored impact molecular characteristics bladder cancer vitro experiments found smad6 promoted prognosis bca patients inhibiting proliferation migration bca cells study reveals two novel subtypes bca identifies smad6 highly promising therapeutic target