Weijie Zhao
How to encourage students in learning chemistry?
National science review
efficient methods extraction features interest remain one biggest challenges interpretation cryoelectron tomograms various automated approaches proposed many work well highcontrast datasets features interest easily detected clearly separated one another inner ear stereocilia cryoelectron tomographic datasets characterized dense array hexagonally packed actin filaments frequently crossconnected features make automated segmentation challenging aggravated highnoise environment cryoelectron tomograms high complexity densely packed features using prior knowledge actin bundle organization placed layers highly simplified ballandstick actin model first obtain global fit density map followed regional local adjustments model show volumetric model building allows us deal high complexity also provides precise measurements statistics actin bundle volumetric models also serve anchoring points local segmentation case actinactin cross connectors volumetric model building particularly augmented computerbased automated fitting approaches powerful alternative conventional automated segmentation approaches successful