Edward James R Gorgon, Mark David S Basco, Almira T Manuel
Teaching evidence based practice in physical therapy in a developing country: a national survey of Philippine schools
BMC medical education
recent studies shown prenatal bpa exposure altered transcriptome profiles autismrelated genes offsprings hippocampus disrupting hippocampal neuritogenesis causing malespecific deficits learning however sex differences effects prenatal bpa exposure developing prefrontal cortex another brain region highly implicated autism spectrum disorder asd investigated obtained transcriptome data rna sequencing analysis prefrontal cortex male female rat pups prenatally exposed bpa control reanalyzed bparesponsive genes associated cortical development social behaviors selected confirmation qrtpcr analysis neuritogenesis primary cells prefrontal cortex pups prenatally exposed bpa control examined social behaviors pups assessed using twotrial threechamber tests malespecific impact downregulation selected bparesponsive gene ie sema5a cortical development vivo interrogated using sirnamediated knockdown utero electroporation technique genes disrupted prenatal bpa exposure associated asd showed sexspecific dysregulation sema5a slc9a9 involved neuritogenesis social behaviors downregulated males anxa2 junb also linked neuritogenesis social behaviors suppressed females neuritogenesis increased males showed strong inverse correlation sema5a slc9a9 expression levels whereas females neuritogenesis decreased correlated anxa2 junb levels sirnamediated knockdown sema5a males also impaired cortical development utero consistent anxa2 junb downregulations deficits social novelty observed female offspring males first study show prenatal bpa exposure dysregulated expression asdrelated genes functions including cortical neuritogenesis development social behaviors sexdependent manner findings suggest besides hippocampus bpa could also exert adverse effects sexspecific molecular mechanisms offsprings prefrontal cortex turn would lead sex differences asdrelated neuropathology clinical manifestations deserves investigation