Jennifer Graebe
Advancing Nursing Practice: The Evolution and Impact of Continuing Professional Development
Journal of continuing education in nursing
describe development genetically defined strain llysine hyperproducing corynebacterium glutamicum systems metabolic engineering wild type implementation 12 defined genomebased changes genes encoding central metabolic enzymes redirected major carbon fluxes desired towards optimal pathway usage predicted silico modeling final engineered c glutamicum strain able produce lysine high yield 055 g per gram glucose titer 120 g l1 lysine productivity 40 g l1 h1 fedbatch culture specific glucose uptake rate wild type could completely maintained engineering process providing highly viable producer key criteria genetically defined strain created study lies maximum limit classically derived producers developed last fifty years first report rationally derived lysine production strain may competitive industrial applications designbased strategy metabolic engineering reported could serve general concept rational development microorganisms efficient cellular factories bioproduction