Yaroslav V Lazur, Sergiy P Kozodayev, Vadym M Roshkanyuk, Alen V Panov
CONSTITUTIONAL AND LEGAL PRINCIPLES OF THE LIMITS OF PERMISSIBLE INTERVENTION IN CONDUCTING BIOMEDICAL RESEARCH WITH HUMAN PARTICIPATION
Wiadomosci lekarskie (Warsaw, Poland : 1960)
maternal overweight obesity associated poor lactation performance including delayed lactogenesis reduced duration however effect human milk composition less well understood evaluated relationship maternal bmi human milk metabolome among guatemalan mothers used data 75 guatemalan mothers participated household air pollution intervention network trial maternal bmi measured 9 20 weeks gestation milk samples collected single time point using aseptic collection one breast 6 mo postpartum analyzed using highresolution mass spectrometry crosssectional untargeted highresolution metabolomics analysis performed coupling hydrophilic interaction liquid chromatography hilic reverse phase c18 chromatography mass spectrometry metabolic features associated maternal bmi determined metabolomewide association study mwas adjusting baseline maternal age education dietary diversity perturbations metabolic pathways identified pathway enrichment analysis mean age participants baseline 2362 381 mean bmi 2427 422 kgm2 total metabolic features detected hilic column 19199 features c18 column 11594 features bmi associated 1026 hilic 500 c18 features enriched pathways represented amino acid metabolism galactose metabolism xenobiotic metabolic metabolism however significant features identified adjusting multiple comparisons using benjaminihochberg false discovery rate procedure fdrbh 02 findings untargeted mwas indicate maternal bmi associated metabolic perturbations galactose metabolism xenobiotic metabolism xenobiotic metabolism cytochrome p450 biosynthesis amino acid pathways significant metabolic pathway alterations detected human milk associated energy metabolismrelated pathways including carbohydrate amino acid metabolismthis trial registered clinicaltrialsgov nct02944682