Hannah R Thompson, Robin Haguewood, Nicole Tantoco, Kristine A Madsen
Lessons Learned: A Strategic Alliance to Improve Elementary Physical Education in an Urban School District
Progress in community health partnerships : research, education, and action
present new approach relating nucleicacid content fluorescence realtime polymerase chain reaction pcr assay coupling twotype branching process pcr fluorescence analog beers law approach reduces bias quantifies uncertainty fluorescence twotype branching process distinguishes complementary strands dna allows stoichiometric description reactions fluorescent probes dna capture initial conditions encountered assays targeting rna analysis expected copynumber identifies additional dynamics occur short times equivalently low cycle numbers investigation variance reveals contributions liquid volume transfer imperfect amplification strandspecific amplification ie one strand synthesized efficiently complement linking branching process fluorescence beers law analog allows priori description background fluorescence also enables uncertainty quantification uq fluorescence turn leads analytical relationships amplification efficiency probability limit detection work sets stage uqpcr input copynumber uncertainty quantified fluorescence kinetics