Urszula Zwierczyk, Mateusz Kobryn, Mariusz Duplaga
The Awareness of the Role of Commercial Determinants of Health and the Readiness to Accept Restrictions on Unhealthy Food Advertising in Polish Society
Nutrients
targeted nextgeneration sequencing ngs rapidly simultaneously detect mutations associated resistance tuberculosis drugs across multiple gene targets use targeted ngs diagnose drugresistant tuberculosis described publicly available data comprehensively reviewed aimed identify targeted ngs assays diagnose drugresistant tuberculosis determine widely technology used assess diagnostic accuracy assays systematic review metaanalysis searched medline embase cochrane library web science core collection global index medicus google scholar clinicaltrialsgov international clinical trials registry platform published unpublished reports targeted ngs drugresistant tuberculosis jan 1 2005 oct 14 2022 updates search embase google scholar feb 13 2024 studies eligible systematic review described targeted ngs approaches predict drug resistance mycobacterium tuberculosis infections using primary samples reference strain collections cultured isolates individuals presumed confirmed tuberculosis search limitations study type language although reports english german french screened eligibility metaanalysis included test accuracy studies used reference standard assessed risk bias using quality assessment diagnostic accuracy studies2 tool primary outcomes metaanalysis sensitivity specificity targeted ngs diagnose drugresistant tuberculosis compared phenotypic genotypic drug susceptibility testing used bayesian bivariate model generate summary receiver operating characteristic plots diagnostic accuracy measures overall stratified drug sample type study registered prospero crd42022368707 identified screened 2920 reports 124 eligible systematic review including 37 review articles 87 reports studies collecting samples targeted ngs sequencing mainly done usa 14 16 87 western europe ten 11 india ten 11 china nine 10 included 24 test accuracy studies metaanalysis 23 different tuberculosis drugs drug groups assessed covering firstline drugs injectable drugs fluoroquinolones predominantly comparing targeted ngs phenotypic drug susceptibility testing combined sensitivity targeted ngs across drugs 941 95 credible interval cri 909963 specificity 981 970989 sensitivity individual drugs ranged 765 525923 capreomycin 991 983997 rifampicin specificity ranged 931 880963 ethambutol 994 983998 amikacin diagnostic accuracy similar primary clinical samples culture isolates overall rifampicin isoniazid ethambutol streptomycin fluoroquinolones similar excluding studies high risk bias overall sensitivity 952 95 cri 917971 specificity 986 974993 targeted ngs highly sensitive specific detecting drug resistance across panels tuberculosis drugs performed directly clinical samples paucity data performance currently recommended drugs barriers preventing use targeted ngs diagnose drugresistant tuberculosis highburden countries need addressed national institutes allergy infectious diseases swiss national science foundation