Ankur Segon, Yogita Sharma-Segon
The Impact of Asking Medical Students What They Want to Learn on Student Participation in Lectures
Cureus
idiopathic pulmonary fibrosis ipf fatal disease unknown etiology poor prognosis characterized lack effective diagnostic therapeutic interventions role immunity pathogenesis ipf significant yet remains inadequately understood study aimed identify potential key genes ipf relationship immune cells integrated bioinformatics analysis verify vivo vitro experiments gene microarray data obtained gene expression omnibus geo differential expression analysis differentially expressed genes degs identified subjected functional enrichment analysis utilizing combination three machine learning algorithms specific genes associated idiopathic pulmonary fibrosis ipf pinpointed diagnostic significance potential coregulators elucidated analyzed correlation key genes immune infiltrating cells via singlesample gene set enrichment analysis ssgsea subsequently singlecell rna sequencing data scrnaseq used explore cell types expressed key genes ipf samples finally series vivo vitro experiments conducted validate expression candidate genes western blot wb quantitative realtime pcr qrtpcr immunohistochemistry ihc analysis total 647 degs ipf identified based two datasets including 225 downregulated genes 422 upregulated genes closely related biological functions cell migration structural organization immune cell chemotaxis extracellular matrix cfh fhl2 identified key genes diagnostic accuracy ipf three machine learning algorithms analysis using ssgsea revealed significant association cfh fhl2 diverse immune cells b cells nk cells scrnaseq analysis indicated cfh fhl2 specifically upregulated human ipf tissues confirmed vitro vivo experiments study cfh fhl2 identified novel potential biomarkers ipf potential diagnostic utility future clinical applications subsequent investigations functions genes ipf interactions immune cells may enhance comprehension diseases pathogenesis facilitate identification therapeutic targets