James W Hicks, Tobias Wang
Safety factors as a 'design' principle of animal form and function: an historical perspective
The Journal of experimental biology
inflamed pulp decreased amount protein amino acids larginine semiessential amino acid production insufficient oxidative stress inflammation larginine sole substrate nitric oxide synthase nos produce nitric oxide plays important role cell proliferation migration study aims evaluate proliferation migration rate hdpscs larginine supplementation serumstarved hdpscs divided four groups control hdpscs dulbeccos modified eagle medium hdpscs 300 moll larginine based culture media group hdpscs 400 moll larginine based culture media group hdpscs 500 moll larginine based culture media group planted two separate 24wellplates 5x104 cellwell proliferation migration evaluation proliferation groups measured using cell count test haemacytometer manual checker 24 h migratory speed rate groups measured using cell migration assay scratch wound assay 24 h cell characteristics evaluated microscope inverted microscope zeiss observer z1 uk read imagej interpretation image j represented measurement migratory speed rate nmh data statistical analysis conducted using oneway anova post hoc bonferroni p005 proliferation post hoc lsd p005 migration ibm spss statistics software version 220 statistically significant difference hdpsc proliferation among various concentration groups larginine based solution 300 400 500 moll compared control group statistically significant difference migratory speed rate hdpscs 500 moll larginine based solution group compared lower concentrations control group highest potency hdpsc proliferation migration observed 500 moll group eej20230108