Claudia Zimmermann, Thomas Waldhoer, Eva Schernhammer, Susanne Strohmaier
Mortality of working-age physicians compared to other high-skilled occupations in Austria from 1998 to 2020
Scandinavian journal of work, environment & health
counting transcripts mrna key method observation modern biology advances counting transcripts single cells singlecell rna sequencing scrnaseq data routinely used identify cells transcriptional profile identify genes differential cellular expression total number transcripts counted per cell vary technical reasons first step many commonly used scrnaseq workflows normalize sequencing depth transforming counts proportional abundances primary objective step reshape data cells similar biological proportions transcripts end similar transformed measurements growing concern normalization transformations result unintended distortions hinder analyses interpretation results led intense focus optimizing methods normalization transformation scrnaseq data take alternative approach avoiding normalization transformation altogether abandon use distances compare cells instead use restricted algebra motivated measurement theory abstract algebra preserves count nature data demonstrate restricted algebra sufficient draw meaningful practical comparisons gene expression use dot product elementary operations approach sidesteps many problems common transformations added benefit simpler intuitive implement approach package countland available python r