Cameron J Gettel, Carlos A Camargo, Christopher L Bennett, D Mark Courtney, Amy H Kaji, Gregory J Fermann, Fiona E Gallahue, Lewis S Nelson, Carleigh F Hebbard, Craig Rothenberg, Ali S Raja, Arjun K Venkatesh
Estimating the size and scope of the academic emergency physician workforce
Academic emergency medicine : official journal of the Society for Academic Emergency Medicine
intracellular bacterial pathogen chlamydia trachomatis undergoes developmental cycle consisting two morphologically discrete developmental forms nonreplicative elementary body eb initiates infection host inside eb differentiates reticulate body rb rb undergoes multiple rounds replication differentiating back infectious eb form cycle essential chlamydial survival failure switch cell types prevents either host invasion replication limitations genetic techniques due obligate intracellular nature chlamydia hampered identification molecular mechanisms involved celltype development designed novel dual promoterreporter plasmid system conjunction livecell microscopy allows visualization cell type switching real time identify genes involved regulation celltype development livecell promoterreporter system leveraged development forward genetic approach combining chemical mutagenesis dual reporter strain imaging tracking chlamydia altered developmental kinetics followed clonal isolation mutants forward genetic workflow flexible tool modified directed interrogation wide range genetic pathways