Amy Turner, Kathleen Huth, Donna Luff, Benjamin Zendejas, Lori R Newman, Alan M Leichtner
Lack of standardization and faculty development in pediatric colonoscopy: A qualitative study
Journal of pediatric gastroenterology and nutrition
heartwater noncontagious tickborne disease domestic wild ruminants data regarding complex processes involved pathogenvectorhost interaction ehrlichia ruminantium infection lacking could improved knowledge associated gene expression changes pathogen host thus current study aimed identify e ruminantium genes upregulated pathogen enters host disease established identification genesproteins may aid future vaccine development strategies heartwater rnasequencing used identify e ruminantium genes exclusively expressed tick bite site sheep skin biopsies sb adult tick salivary glands sg rna extracted pooled samples sb sg collected different time points tick attachment prior disease manifestation ribosomal rna rrna removed samples sequenced several e ruminantium genes highly expressed samples others exclusively expressed concluded e ruminantium genes exclusively expressed sb sb sg compared transcriptome datasets bovine elementary bodies bovebs cell culture may considered early antigenic targets host immunity silico immunogenic epitope prediction analysis preliminary characterization selected genes vitro using elispot assay showed could possibly ideal targets future vaccine development heartwater however epitope characterization still required