Tracy Morton, Daniel J Kim, Tracey Deleeuw, Jason Curran, Paul Olszynski, Virginia W Robinson
Use of point-of-care ultrasound in rural British Columbia: Scale, training, and barriers
Canadian family physician Medecin de famille canadien
human induced pluripotent stem cellderived cardiomyocytes hipsccms frequently used preclinical cardiotoxicity testing remain important tool confirming modelbased predictions drug effects accordance comprehensive vitro proarrhythmia assay cipa despite considerable benefits hipsccms provide concerns surrounding experimental reproducibility emerged investigated effects temporal changes experimental parameters hipsccm electrophysiology icell cardiomyocytes2 cultured biosignals acquired using microelectrode array mea system 114 days continuous recordings revealed 226 increase beating rate 77 decrease field potential duration fpd 20minute equilibration period location specific differences across multiwell plate also observed icell cardiomyocytes2 outer rows beating 88 beats per minute bpm faster inner rows cardiac endpoints also impacted cell culture duration 214 days beating rate decreased 127 bpm fpd lengthened 257 ms spike amplitude increased 33 mv cell culture duration 410 days also impacted cardiomyocyte drug responsiveness e4031 nifedipine isoproterenol qrtpcr results suggest daily variations cardiac metrics may linked continued maturation hipsccms culture 230 days daily experiments also repeated using second cell line cor4u collectively study highlights multiple sources variability consider address performing hipsccm mea studies improve reproducibility data interpretation meabased studies establish standardized protocol report key experimental conditions eg cell line culture time equilibration time electrical stimulation settings raw data values