Richa Mukerjee, Jana L Hirschtick, Luis Zavala Arciniega, Yanmei Xie, Geoffrey D Barnes, Douglas A Arenberg, David T Levy, Rafael Meza, Nancy L Fleischer, Steven F Cook
ENDS, Cigarettes, and Respiratory Illness: Longitudinal Associations Among U.S. Youth
American journal of preventive medicine
calcium ca sparks elementary units subcellular ca release cardiomyocytes cells accordingly ca spark imaging essential tool understanding physiology pathophysiology ca handling used identify new drugs targeting carelated cellular dysfunction eg cardiac arrhythmias large volumes imaging data produced experiments require accurate highthroughput analysis developed new software tool sparkmaster 2 sm2 analysis ca sparks imaged confocal linescan microscopy combining high accuracy flexibility userfriendliness sm2 distributed standalone application requiring installation controlled using simpletouse graphical user interface using python scripting sm2 shown following strengths 1 high accuracy identifying ca release events clearly outperforming previous highly successful software sparkmaster 2 multiple types ca release events identified using sm2 ca sparks waves miniwaves long sparks 3 sm2 accurately split analyze individual sparks within spark clusters capability handled adequately prior tools demonstrate practical utility sm2 two case studies investigating ca levels affect spontaneous ca release largescale release events may promote release refractoriness sm2 also useful atrial smooth muscle myocytes across different imaging conditions sparkmaster 2 new muchimproved userfriendly software accurate highthroughput analysis linescan ca spark imaging data free easy use provides valuable builtin features facilitate visualization analysis interpretation ca spark data enhance quality throughput ca spark wave analysis across cell types particularly study arrhythmogenic ca release events cardiomyocytes