Dharani Keyan, Dusan Hadzi-Pavlovic, Aemal Akhtar, Katie Dawson, Phiona Naserian Koyiet, Richard Bryant
Dimensionality of the Swahili version of the General Health Questionnaire (GHQ-12) in a Kenyan population: A confirmatory factor analysis
Global mental health (Cambridge, England)
although longread sequencing enables generation complete genomes unculturable microbes high cost limits widespread adoption longread sequencing largescale metagenomic studies alternative method assemble shortreads longrange connectivity costeffective way generate highquality microbial genomes develop pangaea bioinformatic approach designed enhance metagenome assembly using shortreads longrange connectivity pangaea leverages connectivity derived physical barcodes linkedreads virtual barcodes aligning shortreads longreads pangaea utilizes deep learningbased read binning algorithm assemble cobarcoded reads exhibiting similar sequence contexts abundances thereby improving assembly high mediumabundance microbial genomes pangaea also leverages multithresholding algorithm strategy refine assembly lowabundance microbes benchmark pangaea linkedreads combination short longreads simulation data mock communities human gut metagenomes pangaea achieves significantly higher contig continuity well nearcomplete metagenomeassembled genomes ncmags existing assemblers pangaea also generates three complete circular ncmags human gut microbiomes