Patricia Wong, Lisa J Meltzer, David Barker, Sarah M Honaker, Judith A Owens, Jared M Saletin, Azizi Seixas, Kyla L Wahlstrom, Amy R Wolfson, Mary A Carskadon
The associations between instructional approach, sleep characteristics and adolescent mental health: Lessons from the COVID-19 pandemic
Sleep health
nanoporebased dna sequencing crisprcasbased gene editing represent groundbreaking innovations molecular biology genomics offering unprecedented insights tools working genetic information students reading editing even writing dna part everyday life developed laboratory procedure includes biosynthesis guide rna ii targeting cas9 specifically linearize pbr322 plasmid iii identification cutting site nanopore dna sequencing protocol intentionally kept simple requires neither living organisms biosafety laboratories divided experimental procedures separate activities facilitate customization assuming access wellequipped molecular biology laboratory initial investment approximately 2700 necessary material costs experiment group amount around 130 furthermore developed freely accessible website httpsdnalesenhsmittweidade sequence read analysis visualization lowering required computational skills minimum strong computational skills provide instructions terminalbased data processing presented activities aim provide handson experiment engages students modern molecular genetics motivates discuss potential implications complete experiment accomplished within half day successfully implemented us high schools teacher training universities tip combine crisprcas gene targeting nanoporebased dna sequencing tool provide website facilitates sequence data analysis visualization