Madeline R Horan, Deo Kumar Srivastava, Jaesung Choi, Kevin R Krull, Gregory T Armstrong, Kirsten K Ness, Melissa M Hudson, Justin N Baker, I-Chan Huang
Multilevel Characteristics of Cumulative Symptom Burden in Young Survivors of Childhood Cancer
JAMA network open
metabolomics emerging powerful technology offers comprehensive view organisms physiological status although widely applied human medicine recently making introduction veterinary medicine result validated metabolomics protocols feline medicine lacking moment since biological interpretation metabolomics data misled extraction method used species matrixspecific optimized validated metabolomic protocols sorely needed systematic optimization performed using fractional factorial experiments serum n 57 urine n 24 evaluating dilution matrices aliquot solvent volume protein precipitation time temperature serum targeted n 76 untargeted n 1949 validation serum respectively excellent instrumental intraassay interday precision observed cv 15 30 respectively linearity deemed sufficient targeted untargeted r2 099 090 respectively appropriate targeted recovery 70 130 achieved targeted n 69 untargeted n 2348 validation urinary protocol excellent instrumental intraassay precision obtained cv 15 30 respectively subsequently discriminative ability metabolomics methods confirmed feline chronic kidney disease ckd univariate statistics n 41 significant metabolites serum n 55 urine pvalue005 validated oplsda models r2y 095 q2y 065 pvalue0001 matrices study first present optimized validated wholistic metabolomics methods feline serum urine using ultrahigh performance liquid chromatography coupled quadrupoleorbitrap highresolution mass spectrometry robust methodology opens avenues biomarker panel selection deeper understanding feline ckd pathophysiology feline applications