Sarah Delorme
[Not Available]
Revue medicale suisse
coordinated events calcium ca2 released endoplasmic reticulum er key second messengers excitable cells painsensing dorsal root ganglion drg neurons events observed ca2 sparks produced combination ryanodine receptors ryr inositol 145triphosphate receptors ip3r1 microscopic signals offer neuronal cells possible means modulating subplasmalemmal ca2 handling initiating vesicular exocytosis superresolution dstorm expansion microscopies visualised nanoscale distributions ryr ip3r1 featured loosely organised clusters subplasmalemmal regions cultured rat drg somata adapted novel correlative microscopy protocol examine nanoscale patterns ryr ip3r1 locality ca2 spark found subplasmalemmal sparks correlated relatively small groups ryr whilst larger sparks often associated larger groups ip3r1 data also showed spontaneous ca2 sparks 30 subplasmalemmal cell area consisted channel species 385 times higher density nonactive regions cell taken together observations reveal distinct patterns length scales ryr ip3r1 coclustering contact sites er surface plasmalemma encode positions quantity ca2 released ca2 spark