Deepanjali Deshmukh, Anuradha V Patil, Rajrajeshwar Deore, Manik C Bhise
Impact of COVID-19 Pandemic on Postgraduate Psychiatry Teaching- Learning and Evaluation in India: A Nationwide Survey
Indian journal of psychological medicine
oral squamous cell cancer oscc prevalent malignancy oral cavity accounting nearly 90 oral malignancies ranks sixth among common types cancer worldwide responsible approximately 145000 deaths year widely accepted noncoding rnas participate cancer development competitive regulatory interaction knowing competing endogenous rna cerna network whereby long noncoding rna lncrna function decoys micrornas regulate gene expression lncrna foxd2as1 reported exert oncogenic role oscc nevertheless cerna network mediated foxd2as1 investigated yet study aimed explore effect foxd2as1 oscc cell process underlying cerna mechanism foxd2as1 expression oscc cells determined via reverse transcription quantitative polymerase chain reaction short hairpin rna targeting foxd2as1 transfected oscc cells silence foxd2as1 expression lossoffunction experiments n 3 assay performed measure cell proliferation apoptosis migration invasion using colony formation tdtmediated dutp nickend labeling wound healing transwell assays respectively rna binding relation verified rna immunoprecipitation luciferase reporter assays rescue experiments designed validate whether foxd2as1 affects cell behavior via gene cellular retinoic acid binding protein 2 crabp2 statistics processed graphpad prism 60 software spss software foxd2as1 significantly upregulated cal27 scc9 cells 68 64 folds response foxd2as1 knockout oscc cell proliferation migration invasion suppressed approximately 50 decrease oscc cell apoptosis enhanced twofold increase foxd2as1 interacted mir378 g alter crabp2 expression crabp2 upregulation partly rescued p 005 p 001 p 0001 inhibitory impact foxd2as1 depletion malignant characteristics oscc cells foxd2as1 enhances oscc malignant cell behaviors interacting mir378 g regulate crabp2 expression