Chunxu Chen, Kay Shannon, Sara Napier, Stephen Neville, Jed Montayre
Ageism directed at older nurses in their workplace: A systematic review
Journal of clinical nursing
nanometrescale cellular information obtained superresolution microscopies often unaccompanied functional information particularly transient diffusible signals life orchestrated nanomicrometre spatial scale describe correlative imaging protocol allows ubiquitous intracellular second messenger calcium ca2 directly visualised nanoscale patterns ryanodine receptor ryr ca2 channels give rise ca2 signals wildtype primary cells achieved combining total internal reflection fluorescence tirf imaging elementary ca2 signals subsequent dnapaint imaging ryrs report straightforward image analysis protocol feature extraction image alignment correlative datasets demonstrate data used visually identify ensembles ca2 channels locally activated genesis cytoplasmic ca2 signals