Mengistu Abayneh, Molla Asnake, Dassalegn Muleta, Asnake Simieneh
Assessment of Bacterial Profiles and Antimicrobial Susceptibility Pattern of Isolates Among Patients Diagnosed with Surgical Site Infections at Mizan-Tepi University Teaching Hospital, Southwest Ethiopia: A Prospective Observational Cohort Study
Infection and drug resistance
pancreatic ductal adenocarcinoma pdac remains challenging disease due complex biology aggressive behavior urgent need efficient therapeutic strategies assess therapy response preclinical pdac organoidbased models combination accurate realtime monitoring required established stable liveimaging organoidperipheral blood mononuclear cells pbmcs cocultures introduced organoidnet deeplearningbased algorithm capable analyzing brightfield images murine human patientderived pdac organoids acquired livecell imaging investigated response chemotherapy gemcitabine pdac organoids pdl1 inhibitor atezolizumab cultured without hlamatched pbmcs time results obtained organoidnet validated endpoint proliferation assay celltiterglo live cell imaging combination organoidnet accurately detected sizespecific drug responses organoids gemcitabine time showing large organoids prone cytotoxic effects approach also allowed distinguishing healthy unhealthy status measuring eccentricity organoids reaction therapy furthermore imaging new organoidspbmcs sandwichbased coculture enabled longitudinal analysis organoid responses atezolizumab showing increased potency pbmcs tumorkilling organoidindividual manner atezolizumab added optimized pdac organoid imaging analyzed organoidnet represents platform capable accurately detecting organoid responses standard pdac chemotherapy time moreover organoidimmune cell cocultures allow monitoring organoid responses immunotherapy offering dynamic insights treatment behavior within coculture setting pbmcs setup holds promise realtime assessment immunotherapeutic effects individual patientderived pdac organoids