Renz C W Klomberg, Astrid E Hellendoorn, Polychronis Kemos, Dimitris Rizopoulos, Frank M Ruemmele, Nicholas M Croft, Lissy de Ridder
Rare and severe adverse events in children with inflammatory bowel disease: analysis of data from the PIBD-SETQuality Safety Registry
The Lancet. Child & adolescent health
seedbearing plants basis seed fruit formation pollination normal progression pollination advances continuous signal exchange material transfer occur mainly female reproductive organs thus molecular mechanism development female reproductive organs vital understanding principle pollination however molecular biology studies development female cones related pollination rare unclear gymnosperms especially cupressaceae study platycladus orientalis monotypic genus within cupressaceae chosen examine female cone transcriptomes prepollination pollination stages illumina pairedend sequencing technology de novo sequence six libraries 3 biological replicates libraries used construct p orientalis transcriptome database containing 71669 unigenes 4963 upregulated unigenes 11747 downregulated unigenes pollination stage subsequent analysis based annotations expression levels functions differentially expressed unigenes enriched pathways developmental processes female cones analysed detail preliminary development pollination mechanism female cone targeted investigations specifically performed determine elementary mechanism secretion functioning pollination drop vital ovule secretion pollination stage ultimately expression 15 unigenes selected two stages assessed confirmed using qrtpcr demonstrated reliable data significant differences expression profiles key genes one largest available transcriptomic resources species database constructed prospectively adapt physiological genomic data woody plants work provided first transcriptome profile p orientalis female cones different developmental stages promote illumination pollination mechanism p orientalis serve basis indepth genomic study cupressaceae family initiative arouse interest attention scholars pave way future studies