Tomás Reyes-Amigo, Jessica Ibarra-Mora, Nicolás Aguilar-Farías, Nicolás Gómez-Álvarez, Hernaldo Carrasco-Beltrán, Rafael Zapata-Lamana, Juan Hurtado-Almonácid, Jacqueline Páez-Herrera, Rodrigo Yañez-Sepulveda, Guillermo Cortés, Grissel Rolle-Cáceres, Andréa Bezerra
An active break program (ACTIVA-MENTE) at elementary schools in Chile: study protocol for a pilot cluster randomized controlled trial
Frontiers in public health
dna base damage major source oncogenic mutations disruption gene expression stalling rna polymerase ii rnap sites dna damage subsequent triggering repair processes major roles shaping genomewide distribution mutations clearing barriers transcription minimizing production miscoded gene products despite importance genetic integrity key mechanistic features transcriptioncoupled repair tcr process controversial unknown exploited wellpowered vivo mammalian model system explore mechanistic properties parameters tcr alkylation damage fine spatial resolution discrimination damaged dna strand rigorous interpretation generalizable mathematical model dna damage tcr developed fitting experimental data model simulation revealed rna polymerases frequently bypass lesions without triggering repair indicating small alkylation adducts unlikely efficient barrier gene expression following burst damage efficiency transcriptioncoupled repair gradually decays gene bodies implications occurrence accurate inference driver mutations cancer reinitation transcription repair site general feature transcriptioncoupled repair observed data consistent reinitiation never taking place collectively results reveal directional stochastic activity tcr shapes distribution mutations following dna damage