Celia Aradillas-García, Gabriela Palos-Lucio, Aldanely Padrón-Salas
Socio-Urban Spatial Patterns Associated with Dyslipidemia among Schoolchildren in the City of San Luis Potosi, Mexico
Journal of urban health : bulletin of the New York Academy of Medicine
shanxi aged vinegar microbiome encodes wide variety bacteriocins aim study mine screen characterize novel broadspectrum bacteriocins largescale microbiome data shanxi aged vinegar machine learning molecular simulation activity validation total 158 potential bacteriocins innovatively mined 117552 representative genes based metatranscriptomic information shanxi aged vinegar microbiome using machine learning techniques 12 microorganisms identified secrete bacteriocins genus level subsequently employing alphafold2 structure prediction molecular dynamics simulations eight bacteriocins high stability screened confirmed bacteriostatic activity escherichia coli bl21 expression system gene386319 named lab3 gene403047 named lab4 strongest antibacterial activities purified twostep methods analyzed mass spectrometry two bacteriocins broadspectrum antimicrobial activity minimum inhibitory concentration values 679 gml1531 gml staphylococcus aureus escherichia coli furthermore molecular docking analysis indicated lab3 lab4 could interact dihydrofolate reductase hydrogen bonds saltbridge forces hydrophobic forces findings suggested two bacteriocins could considered promising broadspectrum antimicrobial agents