L Humphreys, C Bigby, T Araten-Bergman, T Iacono
Active Support Measure: a multilevel exploratory factor analysis
Journal of intellectual disability research : JIDR
naturally occurring isolates baculoviruses bombyx mori nucleopolyhedrovirus bmnpv usually consist numerous genetically different haplotypes deciphering different haplotypes isolates hampered large size dsdna genome well short read length next generation sequencing ngs techniques widely applied baculovirus isolate characterization study addressed challenge combining accuracy ngs determine single nucleotide variants snvs genetic markers long read length nanopore sequencing technique hybrid approach allowed comprehensive analysis genetically homogeneous heterogeneous isolates bmnpv specifically allowed identification two putative major haplotypes heterogeneous isolate bmnpvja snv position linkage snv positions determined based ngs data linked long nanopore reads position weight matrix using modified expectationmaximization algorithm nanopore reads assigned according occurrence variable snv positions machine learning cohorts reads de novo assembled led identification bmnpv haplotypes method demonstrated strength combined approach short longread sequencing techniques decipher genetic diversity baculovirus isolates