Sanni Pöysä, Eija Pakarinen, Marja-Kristiina Lerkkanen
Profiles of Work Engagement and Work-Related Effort and Reward Among Teachers: Associations to Occupational Well-Being and Leader-Follower Relationship During the COVID-19 Pandemic
Frontiers in psychology
structure nlinked glycosylation important quality attribute therapeutic monoclonal antibodies different carbon sources cell culture media mannose galactose reported different influences glycosylation patterns accurate prediction control glycosylation profile important process development mammalian cell cultures study mathematical model named glycan residues balance analysis greba developed based concept elementary flux mode efm used predict glycosylation profile steady state cell cultures experiments carried pseudoperfusion cultivation antibody producing chinese hamster ovary cho cells various concentrations combinations glucose mannose galactose cultivation cho cells mannose combinations mannose galactose resulted decreased lactate ammonium production matured glycosylation patterns compared cultures glucose furthermore growth rate igg productivity similar conditions cells cultured galactose alone lactate fed well used complementary carbon source leading cell growth rate igg productivity comparable feeding sugars data glycoprofiles used training model simulate glycosylation changes varying concentrations mannose galactose study showed greba model good predictive capacity nlinked glycosylation greba used guidance development glycoprotein cultivation processes