Atiye Karakul, Duygu Sönmez Düzkaya, Gamze Bozkul, Murat Çapanoğlu
The effect of mobile game training designed for asthmatic children on asthma management and quality of life
Journal of pediatric nursing
significant imbalance mitochondrial activity oxidative stress os status patients atopic dermatitis ad study aims screen skin peripheral mitochondriarelated biomarkers providing insights underlying mechanisms mitochondrial dysfunction ad public data obtained mitocarta 30 geo database screened mitochondriarelated differentially expressed genes mitodegs using r language performed go kegg pathway analysis mitodegs ppi machine learning algorithms also used select hub mitodegs meanwhile expression hub mitodegs clinical samples verified using roc curve analysis diagnostic performance risk model constructed hub mitodegs evaluated training validation sets computeraided algorithm analyses included gene set enrichment analysis gsea immune infiltration mitochondrial metabolism centered hub mitodegs also used realtime pcr spearman method evaluate relationship plasma circulating cellfree mitochondrial dna ccfmtdna levels disease severity ad patients mitodegs ad significantly enriched pathways involved mitochondrial respiration mitochondrial metabolism mitochondrial membrane transport four hub genes bax idh3a mrps6 gpt2 selected take part creation novel mitochondrialbased risk model ad prediction risk score demonstrated excellent diagnostic performance training cohort auc 1000 validation cohort auc 0810 four hub mitodegs also clearly associated innate immune cells infiltration molecular modifications mitochondrial hypermetabolism ad discovered ad patients considerably greater plasma ccfmtdna levels controls u 920 p 0001 besides significant relationship upregulation plasma mtdna severity ad symptoms study highlights bax idh3a mrps6 gpt2 crucial mitodegs demonstrates efficiency identifying ad moderate severe ad associated increased markers mitochondrial damage cellular stress ccfmtdna study provides data support variation mitochondriarelated functional characteristics ad patients