Patrick Ward, Johann Windt, Thomas Kempton
Business Intelligence: How Sport Scientists Can Support Organization Decision Making in Professional Sport
International journal of sports physiology and performance
spectroscopic singlemolecule localization microscopy ssmlm takes advantage nanoscopy spectroscopy enabling sub10 nm resolution well simultaneous multicolor imaging multilabeled samples reconstruction raw ssmlm data using deep learning promising approach visualizing subcellular structures nanoscale develop novel computational approach leveraging deep learning reconstruct labelfree fluorescencelabeled ssmlm imaging data developed twonetworkmodel based deep learning algorithm termed dssmlm reconstruct ssmlm data effectiveness dssmlm assessed conducting imaging experiments diverse samples including labelfree singlestranded dna ssdna fiber fluorescencelabeled histone markers cos7 u2os cells simultaneous multicolor imaging synthetic dna origami nanoruler labelfree imaging spatial resolution 622 nm achieved ssdna fiber fluorescencelabeled imaging dssmlm revealed distribution chromatinrich chromatinpoor regions defined histone markers cell nucleus also offered simultaneous multicolor imaging nanoruler samples distinguishing two dyes labeled three emitting points separation distance 40 nm dssmlm observed enhanced spectral profiles 88 higher localization detection singlecolor imaging 505 higher localization detection simultaneous twocolor imaging demonstrate feasibility deep learningbased reconstruction ssmlm imaging applicable labelfree fluorescencelabeled ssmlm imaging data anticipate technique valuable tool highquality superresolution imaging deeper understanding dna molecules photophysics facilitate investigation multiple nanoscopic cellular structures interactions