Susan B Racette, Tiffany C Dill, M Leanne White, Jacqueline C Castillo, Mary L Uhrich, Cindi L Inman, Nicholas C DuPont, B Ruth Clark
Influence of physical education on moderate-to-vigorous physical activity of urban public school children in St. Louis, Missouri, 2011-2014
Preventing chronic disease
torin1 selective kinase inhibitor targeting mammalian target rapamycin mtor remains widely used autophagy research due potent autophagyinducing abilities regardless unspecific properties recognizing impact mtor inhibition metabolism objective develop reliable thorough untargeted metabolomics workflow study torin1induced metabolic changes mouse embryonic fibroblast mef cells crucially quality assurance quality control qaqc protocols designed increase confidence reported findings reducing likelihood false positives including validation experiment replicating experimental steps sample preparation data analysis study investigated metabolic fingerprint torin1 exposure using liquid chromatographyhigh resolution mass spectrometry lchrmsbased untargeted metabolomics platforms workflow identified 67 altered metabolites torin1 exposure combining univariate multivariate statistics implementation validation experiment particular intracellular ceramides diglycerides phosphatidylcholines phosphatidylethanolamines glutathione 5methylthioadenosine downregulated lysophosphatidylcholines lysophosphatidylethanolamines glycerophosphocholine triglycerides inosine hypoxanthine upregulated biochemical pathway analyses provided deeper insights reported changes ultimately study provides valuable workflow implemented future investigations effects compounds including specific autophagy modulators