Sasha A Fleary
Linking Childhood Cultural Health Capital Factors with Adult Health Literacy
Health literacy research and practice
although cultured adult cardiac myocytes combination celllevel genetic modifications adopted study protein function cellular alterations caused culture conditions need clarified interpret effects genetically altered proteins systematically compared cellular morphology global ca2 signaling elementary ca2 release sparks arrangement ryanodine receptor ryr clusters shortterm 2 dayscultured adult rat ventricular myocytes freshly isolated myocytes transverse ttubules remarkably decreased 25 culture wholecell capacitance reduced 35 magnitude depolarizationinduced ca2 transients decreased 50 ca2 transient decay slowed culture culture affect sarcoplasmic reticulum sr ca2 loading therefore fractional ca2 release attenuated culture cultured cells ltype ca2 current ica smaller 50 controls inactivation slower cultured myocytes significantly fewer 50 control ca2 sparks local ca2 releases ryr clusters compared freshly isolated cells amplitude kinetics duration timetopeak individual sparks similar showed greater width cultured cells immunolocalization analysis revealed crossstriation ryrs distribution became weaker less organized density ryr clusters decreased cultured myocytes data suggest loss ttubules generation compromised ca2 transients ica shortterm adult ventricular cell culture independent sr ca2 loading status addition deteriorated arrangement ryrclusters decreased density shortterm culture may partly responsible fewer ca2 sparks decrease global ca2 release