Rena F Subotnik, Paula Olszewski-Kubilius, Frank C Worrell
The role of insider knowledge in the trajectories of highly accomplished scientists
Annals of the New York Academy of Sciences
electron microscopy em revolutionized way visualize cellular ultrastructure volume em vem broadened threedimensional nanoscale imaging capacity however intrinsic tradeoffs imaging speed quality em restrict attainable imaging area volume isotropic imaging vem large biological volumes remains unachievable developed emdiffuse suite algorithms designed enhance em vem capabilities leveraging cuttingedge image generation diffusion model emdiffuse generates realistic predictions high resolution ultrastructural details exhibits robust transferability taking one pair images 3 megapixels finetune denoising superresolution tasks emdiffuse also demonstrated proficiency isotropic vem reconstruction task generating isotropic volume even absence isotropic training data demonstrated robustness emdiffuse generating isotropic volumes seven public datasets obtained different vem techniques instruments generated isotropic volume enables accurate threedimensional nanoscale ultrastructure analysis emdiffuse also features selfassessment functionalities predictions reliability envision emdiffuse pave way investigations intricate subcellular nanoscale ultrastructure within large volumes biological systems