Daniela Karine Ramos, Bruna Santana Anastácio, Gleice Assunção da Silva, Leila Urioste Rosso, João Mattar
Burnout syndrome in different teaching levels during the covid-19 pandemic in Brazil
BMC public health
study aims compare internal chemical composition appearance indifferent growth patterns years saposhnikovia divaricata decoction pieceswhich applied explore effect growth patterns years quality appearance characteristic data 55 batches different growth patterns years divaricata collected using pantone color cardhigh performance liquid chromatography hplc used determine contents primoglucosylcinmifugincimifugin4odglucosyl5omethylvisamminol secoglucosylhamaudol content alcohol soluble extract watersoluble extract determined hotdip method content volatile oil determined steam distillation correlation growth patterns years contents 4 chromonesextracts volatile oil analyzed modern statistical methods alsothe method comprehensively evaluating quality chinese herbal pieces developed combining growth patterns yearsappearance chemical indexes mtt assay used evaluate effects survival rate raw264 7 cells four different concentrations chromones lps used stimulate wellgrowing raw264 7 cells establish inflammatory model contents tnf cell supernatant detected test kit elisa method contents alcohol soluble extracts watersoluble extracts different growth patterns years wild productsperennial cultivationannual cultivation contents volatile oil wild productsperennial cultivationannual cultivation contents four chromones wild productsperennial cultivation annual cultivation significant difference sum two indexes pharmacopoeia perennial cultivation wild products 4 chromones showed toxicity raw264 7 cells 5 mgl1 release tnf inhibited 4 chromones antiinflammatory effect cimifugin best summarythere obvious differences appearance characteristicsinternal quality effects different growth patterns years showed wild products superior perennial cultivation perennial cultivation superior annual cultivation order alleviate shortage wild divaricata resourcesit suggested chinese pharmacopoeia standard increase character decoction pieces perennial cultivationand properly raise limit requirement sum two indexes chinese pharmacopoeia ensure clinical demands effect