Khushboo Bisht, Bharathi Mohan, Basavaraj Jatteppanavar, Hannah Theresa Sony, Shailendra Handu, Minakshi Dhar
An observational study of root-cause analysis of medication errors in elderly with methotrexate toxicity
Expert opinion on drug safety
citizen scientistbased environmental monitoring public education becoming increasingly popular however current technologies antibioticbased novel contaminant identification still restricted laboratory sample collection analysis due detection methodologies apparatus limitations study developed timeresolved immunofluorescencebased simultaneous fieldbased assay ciprofloxacin cip enrofloxacin enr matches test results geographic locations assay helps public understand potential levels antibiotic exposures environments helps take appropriate action reduce risk assay developed using smartphones social software addition rapid testing method uses portable lowcost analytical kit smartphone app build fieldbased detection platform detection analysis enr cip water aquatic products methodological evaluation good detection limits 04 ngml 05 ngg enr water fish quantification limits 12 ngml 14 ngg recoveries 890 1010 780 970 cip water fish limits detection 03 ngml 04 ngg limits quantification 09 ngml 12 ngg recoveries 750 910 720 890 coefficients variation 15 limits sufficient prevent two antibiotics crossing simultaneous detection assay validated using real samples assess effectiveness assay platform field deployments results consistent obtained liquid chromatographytandem mass spectrometry lcms enzymelinked immunoassay elisa techniques addition trfia assay process requires less time uses portable instruments less complex traditional methods study provides new scientific accurate rapid detection method antibiotic detection citizen scientists helping scientists obtain wider range data providing opportunities solve scientific problems