Belinda Agyapong, Yifeng Wei, Raquel da Luz Dias, Vincent Israel Opoku Agyapong
Burnout and Associated Psychological Problems Among Teachers and the Impact of the Wellness4Teachers Supportive Text Messaging Program: Protocol for a Cross-sectional and Program Evaluation Study
JMIR research protocols
ulcerative colitis uc autoimmune inflammatory disorder gastrointestinal tract programmed cell death pcd including panoptosis autophagy plays roles inflammation immunity study aimed investigate molecular signature immune landscape panoptosis autophagyrelated differentially expressed genes degs uc analyzing uc dataset gse206285 yielded degs differentially expressed panoptosis autophagyrelated genes identified using degs relevant gene collections functional pathway enrichment analyses conducted proteinprotein interaction ppi network established identify hub genes trrust database predicted transcription factors tfs pivotal mirnas drugs interacting hub genes immune infiltration analysis ucassociated singlecell sequencing data analysis construction competing endogenous rna cerna network hub genes conducted machine learning identified key candidate genes evaluated diagnostic value via receiver operating characteristic roc curves uc mice model verified expression key candidate genes identifying ten panoptosisrelated hub degs four autophagyrelated hub degs associated cell chemotaxis wound healing positive mapk cascade regulation immune infiltration analysis revealed increased immunocyte infiltration uc patients hub genes closely linked various immune cell infiltrations machine learning identified five key candidate genes timp1 timp2 timp3 il6 ccl2 strong diagnostic performance singlecell level genes exhibited high expression inflammatory fibroblasts iafs showed significant expression differences colon mucosa uc patients uc mice model study identified validated novel molecular signatures associated panoptosis autophagy uc potentially influencing immune dysregulation wound healing thus opening avenues future research therapeutic interventions