Deidre O Turner|Shelley J. Williams-Cocks|Ryan Bullen|Jeremy Catmull|Jesse Falk|Danny Bernard Martin|Jarom Mauer|Annabel Barber|Robert C. Wang|Shawn Gerstenberger|Karl Kingsley
High-risk human papillomavirus (HPV) screening and detection in healthy patient saliva samples: a pilot study
University of Nevada, Las Vegas|University of Nevada Reno|University of Nevada, Las Vegas|University of Nevada, Las Vegas|University of Nevada, Las Vegas|University of Nevada, Las Vegas|University of Nevada, Las Vegas|University of Nevada Reno|University of Nevada Reno|University of Nevada, Las Vegas|University of Nevada, Las Vegas
human papillomaviruses hpv large family nonenveloped dna viruses mainly associated cervical cancers recent epidemiologic evidence suggested hpv may independent risk factor oropharyngeal cancers evidence suggests hpv may modulate malignancy process tobacco alcoholinduced oropharynx tumors might also primary oncogenic factor inducing carcinogenesis among nonsmokers evidence however needed regarding oral hpv prevalence among healthy adults estimate risk goal study perform hpv screening normal healthy adults assess oral hpv prevalencehealthy adult patients us dental school selected participate pilot study dna isolated saliva samples screened highrisk hpv strains hpv16 hpv18 processed using qpcr quantification confirm analytical sensitivity specificitychisquare analysis revealed patient sample representative general clinic population respect gender race age p 005 four patient samples found harbor hpv16 dna representing 26 total n 151 three four hpv16positive samples patients 65 years age four female hispanic nonwhite samples tested positive hpv18the successful recruitment screening healthy adult patients revealed hpv16 hpv18 present small subset results provide new information oral hpv status may help contextualize results studies demonstrate oral cancer rates risen us among females minorities geographic areas solely explained rates tobacco alcohol use results study may significant value understanding oral health disease risk well help design future studies exploring role factors influence oral hpv exposure well short longterm consequences oral hpv infection