Elizabeth Peacock-Chambers, Pilar Del Canto, Douglas Ahlers, Mario Valdivia Peralta, Judith Palfrey
School-Based Disaster Recovery: Promotion of Children's Mental Health Over the Long Haul
Disaster medicine and public health preparedness
developed stem cell educator therapy among multiple clinical trials based immune modulations multipotent cord bloodderived stem cells cbscs different compartments immune cells cells monocytesmacrophages type 1 diabetes autoimmune diseases however effects cbscs b cells remained unclear better understand molecular mechanisms underlying immune education cbscs explored modulations cbscs human b cells cbscs isolated human cord blood units confirmed flow cytometry different markers purity b cells purified using anticd19 immunomagnetic beads human peripheral blood mononuclear cells pbmcs next activated b cells treated presence absence coculture cbscs 7 days undergoing flow cytometry analysis phenotypic changes different markers reverse transcriptionpolymerase chain reaction rtpcr utilized evaluate levels galectin expressions cbscs without treatment activated b cells order find key galectin contributing bcell modulation flow cytometry demonstrated proliferation activated b cells markedly suppressed presence cbscs leading downregulation immunoglobulin production activated b cells phenotypic analysis revealed treatment cbscs increased percentage igdcd27 nave b cells decreased percentage igdcd27 switched b cells transwell assay showed immune suppression cbscs b cells dependent galectin9 molecule confirmed blocking experiment antigalectin9 monoclonal antibody mechanistic studies demonstrated calcium levels cytoplasm mitochondria downregulated treatment cbscs causing decline mitochondrial membrane potential activated b cells western blot exhibited levels phosphorylated akt erk12 signaling proteins activated b cells also markedly reduced presence cbscs cbscs displayed multiple immune modulations b cells galectin9mediated mechanism calcium fluxakterk12 signaling pathways data advance current understanding molecular mechanisms underlying stem cell educator therapy treat autoimmune diseases clinics