Hua-Xuan Liu, Bik-Chu Chow, Wei Liang, Holger Hassel, YaJun Wendy Huang
Measuring a Broad Spectrum of eHealth Skills in the Web 3.0 Context Using an eHealth Literacy Scale: Development and Validation Study
Journal of medical Internet research
dna repair protein o6methylguaninedna methyltransferase mgmt known play role sensitivity temozolomide promoter hypermethylation mgmt commonly used predict low expression levels mgmt gliomas despite observed discordance promoter methylation protein levels investigated functional role gene body cytosine modification regulating levels mgmt gene expression sensitivity temozolomide 91 human glioblastoma samples observed significant variation mgmt expression levels patients unmethylated promoter higher levels gene body cytosine modification correlating higher gene expression levels furthermore inducing hypomethylation across mgmt gene body decitabine corresponded decreased levels mgmt gene expression lymphoblastoid glioblastoma cell lines indicating important functional role gene body cytosine modifications maintaining gene expression reasoned decrease mgmt expression induced decitabine may render resistant glioblastoma cell lines sensitive temozolomide consistent reasoning found mgmtexpressing glioblastoma cell lines exhibiting unmethylated mgmt promoter pretreated decitabine became significantly sensitive temozolomide overall results suggest functional role gene body cytosine modification regulating gene expression mgmt indicate pretreating patients whose tumors unmethylated mgmt promoter decitabine temozolomide treatment may increase response therapy