Stimulation of the synthesis of the H1 and H3 histone fractions of mouse epidermis by 12-O-tetradecanoylphorbol-13-acetate.
Histones from mouse epidermis were fractionated chemically by the method of Johns, and the 5 resulting fractions were subjected to acrylamide gel electrophoresis at pH 3.2 using 15% gels. Their migration sequence was observed to be the same as that reported for calf thymus histones. Total epidermal histone from mice treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) and a pulse of [3H]lysine was also subjected to acrylamide gel electrophoresis and 5 separate bands were detected. Large increases in the incorporation of [3H]lysine into the H1 and H3 histones were observed when compared to histone bands from control mice, with smaller but significant increases of incorporation occurring into the H2B, H2A, and H4 fractions.