<p>This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p> <p>We here report the complete nucleotide sequence of the 47.9 kb mitochondrial (mt) genome
from the obligate aerobic yeast <italic>Yarrowia lipolytica</italic>. It encodes, all on the same strand,
seven subunits of NADH: ubiquinone oxidoreductase (ND1-6, ND4L), apocytochrome
<italic>b</italic> (COB), three subunits of cytochrome oxidase (COX1, 2, 3), three subunits of ATP
synthetase (ATP6, 8 and 9), small and large ribosomal RNAs and an incomplete set of
tRNAs. The <italic>Y. lipolytica</italic> mt genome is very similar to the <italic>Hansenula wingei</italic> mt genome,
as judged from blocks of conserved gene order and from sequence homology. The extra
DNA in the <italic>Y. lipolytica</italic> mt genome consists of 17 group 1 introns and stretches of A+Trich
sequence, interspersed with potentially transposable GC clusters. The usual mould mt
genetic code is used. Interestingly, there is no tRNA able to read CGN (arginine) codons.
CGN codons could not be found in exonic open reading frames, whereas they do occur in
intronic open reading frames. However, several of the intronic open reading frames have
accumulated mutations and must be regarded as pseudogenes. We propose that this may
have been triggered by the presence of untranslatable CGN codons. This sequence is
available under EMBL Accession No. AJ307410.</p>
[]
Comp Funct Genomics. 2001 Apr; 2(2):80-90