<p>This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p> <p>We present a strategy to identify and map large numbers of transposon insertions in
the genome of <italic>Caenorhabditis elegans</italic>. Our approach makes use of the mutator strain
<italic>mut-7</italic>, which has germline-transposition activity of the Tc1/mariner family of transposons,
a display protocol to detect new transposon insertions, and the availability of
the genomic sequence of <italic>C. elegans</italic>. From a pilot insertional mutagenesis screen, we
have obtained 351 new Tc1 transposons inserted in or near 219 predicted <italic>C. elegans</italic>
genes. The strategy presented provides an approach to isolate insertions of natural
transposable elements in many <italic>C. elegans</italic> genes and to create a large-scale collection
of <italic>C. elegans</italic> mutants.</p>
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Comp Funct Genomics. 2004 Apr; 5(3):225-229