<p>This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p> <p>Comparative genomics, analyzing variation among individual genomes, is an area of
intense investigation. DNA sequencing is usually employed to look for polymorphisms and
mutations. Pyrosequencing, a real-time DNA sequencing method, is emerging as a popular
platform for comparative genomics. Here we review the use of this technology for mutation
scanning, polymorphism discovery and chemical haplotyping. We describe the methodology
and accuracy of this technique and discuss how to reduce the cost for large-scale analysis.</p>
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Comp Funct Genomics. 2002 Feb; 3(1):51-56