<title>Background</title> <p>High chemical complexity of herbal medicines makes quality control through chemical analysis difficult. For example, a common Chinese medicinal herb <italic>Cortex Cinnamomi </italic>(<italic>Rougui</italic>) revealed over 90 volatile components in a gas chromatography-mass spectroscopy (GC-MS) experiment, only 60 of which were chemically identified [##REF##15019037##1##]. Little chemical information is known about herbal medicines [##UREF##0##2##]. Mok and Chau categorized authentication and quality control of herbal medicines into the 'component-based' and 'pattern-based' approaches [##UREF##1##3##]. For a refined classification, we refer to these two approaches in this article as the compound-oriented approach and pattern-oriented approach respectively. The compound-oriented approach includes the marker approach and the multi-compound approach. The marker approach takes into account herbal medicines with known components, while the multi-compound approach may include some unknown components whose chemical properties are known. The pattern-oriented approach, such as fingerprint analysis, evaluates all data acquired from analytical instrument. For example, fingerprints and bioactivities have recently been correlated to the quality control of herbal medicines [##REF##16213118##4##, ####UREF##2##5##, ##REF##16132135##6####16132135##6##]. Instead of measuring all elements within a biological system, systems biology aims to reveal intrinsic trends of the system [##REF##12931164##7##,##REF##15889489##8##]. Metabonomics focuses on the investigation of high-throughput data from metabolite profiling. The key advantage of metabonomics is to provide an integrative and systematic view of metabolism, which may also reveal the quality of herbal medicines [##REF##15656647##9##,##REF##16939360##10##].</p> <p>Different ingredients within a herbal medicine may have synergistic effects. These active ingredients must be identified and quantified for better understanding of the action mechanisms of herbal medicines. For the moment, the conventional marker approach is not successful as only a few chemical markers are available for herbal medicines in the American Herbal Pharmacopeia and Chinese Pharmacopoeia. For example, in the Chinese Pharmacopoeia (2005 edition) only one chemical marker chlorogenic acid (C<sub>16</sub>H<sub>18</sub>O<sub>9</sub>) is recommended for the identification of <italic>Flos Lonicerae </italic>(<italic>Jinyinhua</italic>) (content ≥ 1.5%) and <italic>Flos Chrysanthemi </italic>(<italic>Juhua</italic>) (content ≥ 0.2%) [##UREF##3##11##]. These two herbs cannot be differentiated with the only chemical marker. Likewise, <italic>Fufang Danshen Diwan </italic>is a formulated herbal medicine that consists of three herbs but there is only one chemical marker salvianic acid A (C<sub>9</sub>H<sub>10</sub>O<sub>5</sub>) recommended for this particular medicine in the Chinese Pharmacopoeia (2005 edition) [##UREF##3##11##].</p> <p>The pattern-oriented approach such as fingerprinting is more useful than compound-oriented approach in most cases. Chemical fingerprints are characteristic for herbal medicines, and are therefore useful in the quality control of herbal medicines [##REF##16472540##12##, ####UREF##4##13##, ##UREF##5##14##, ##UREF##6##15####6##15##]. The information-rich chemical fingerprints can be obtained from advanced instruments [##UREF##7##16##, ####UREF##8##17##, ##UREF##9##18##, ##UREF##10##19##, ##REF##15620519##20##, ##UREF##11##21####11##21##]. All detectable chemical components of a herbal medicine may be shown in fingerprints. Chemometrics helps analyze and interpret useful information from raw data, e.g. alignment of shifts of retention time in chromatography, data assessment and comparison, smoothing and filtering, deconvolution and resolution of overlapping peak clusters, in determination of the chromatographic and spectral profiles of pure components [##UREF##12##22##, ####REF##16174438##23##, ##REF##15032363##24##, ##UREF##13##25##, ##UREF##14##26##, ##UREF##15##27####15##27##]. This article summarizes recent advances in both compound-oriented and pattern-oriented approaches in terms of experimentation, instrumentation and data processing.</p> <title>Compound-oriented approach</title> <title>Marker approach</title> <p>According to the Chinese Pharmacopoeia (2005 edition), identification and quantification of chemical markers are crucial to the quality control of herbal medicines. A total of 525 quantitative assays of chemical markers were documented in the Chinese Pharmacopoeia (2005 edition) for assessment of herbal medicinal materials, plant lipids, herbal extracts and formulations [##UREF##3##11##]. Chemistry of these markers is known and their analytical procedures and reference standards are available for quality control. Chau <italic>et al</italic>. used near infrared spectroscopy (NIR) to quantitatively determine the content of berberine and total alkaloid in <italic>Cortex Phellodendri </italic>(<italic>Guanhuangbo</italic>) [##REF##17512816##28##]. The content of berberine determined by high-performance liquid chromatography-diode array detection (HPLC-DAD) was used as a critical parameter to confirm the accuracy of the data obtained from NIR according to a linear model of partial least squares (PLS) regression. In another study, high-performance liquid chromatography (HPLC) and HPLC-DAD were used to assess the quality consistency of a formulated Chinese medicine <italic>Qingfu Guanjie Shu </italic>(capsule) using four marker compounds, namely sinomenine, paeoniflorin, paeonol and curcumin. [##UREF##16##29##]. Lin <italic>et al</italic>. used liquid chromatography-tandem mass spectrometers (LC-MS/MS), solid phase extraction, and the marker glycyrrhetic acid to simultaneously validate <italic>Radix Glycyrrhizae </italic>(<italic>Gancao</italic>) and quantify the target compound in the samples [##UREF##17##30##]. Quantitative studies of markers and identification of active ingredients were carried out for the quality control of herbal medicines [##UREF##18##31##, ####REF##16515793##32##, ##REF##17029978##33##, ##UREF##19##34####19##34##]. Gas chromatography (GC), gas chromatography-mass spectroscopy (GC-MS), thin layer chromatography (TLC), thin layer chromatography-ultraviolet spectrophotometry (TLC-UV), capillary electrophoresis (CE) and capillary zone electrophoresis (CZE) were also proposed for the quality control of herbal medicines [##UREF##20##35##, ####UREF##21##36##, ##UREF##22##37##, ##UREF##23##38##, ##UREF##24##39####24##39##].</p> <title>Multi-compound approach</title> <p>Compared with the marker approach, the multi-compound approach uses multiple compounds with known chemical properties and does not necessarily require chemical markers. Chemometric deconvolution and resolution are major methods in this approach. In the Chinese Pharmacopoeia (2005 edition) [##UREF##3##11##], multiple compounds, instead of a single compound, are recommended for the quality control of herbal medicines. For example, total flavonol glycosides (i.e. quercetin, isorhamnetin and kaempferol) as well as total terpene lactones (i.e. bilobalide, and ginkgolides A, B and C) were used for the quality control of a ginkgo leaf product [##UREF##3##11##]. However, analyzing multiple compounds in a single chromatogram may not be easy. These chromatograms often contain overlapping peaks, which may not be resolved by changing chromatographic conditions. One possible solution is the use of chemical and/or instrumental methods that take advantage of spectra with very close retention times, e.g. mass spectra, ultraviolet spectra or other chemical properties containing variations large enough to resolve overlapping chromatographic profiles [##REF##15336357##40##, ####UREF##25##41##, ##REF##16168606##42##, ##UREF##26##43####26##43##].</p> <p>Chemometric resolution methods (CRM) were used extensively in the past decades to 'purify' chromatographic peak profiles of complex mixture systems such as herbal medicines [##UREF##4##13##]. The qualitative and quantitative chemical information obtained by CRM did help discover the active ingredients of herbal medicines and study the synergistic effects of the ingredients [##REF##15019037##1##].</p> <p>Previously, both iterative and non-iterative resolution methods were used to study the volatile and non-volatile components in herbal medicines [##REF##16539851##44##, ####REF##11206787##45##, ##REF##14601831##46####14601831##46##]. Many non-iterative resolution methods such as heuristic evolving latent projection (HELP), alternative moving window factor analysis (AMWFA), (subwindow factor analysis) SFA, evolving window orthogonal projection (EWOP) were useful in discovering more than ten components of herbal medicines [##REF##15137973##47##, ####UREF##27##48##, ##REF##16430907##49##, ##REF##15478995##50##, ##REF##11269523##51####11269523##51##]. Using GC-MS coupled with HELP, Li <italic>et al</italic>. identified 38 volatile chemical components of <italic>Radix Paeoniae Rubra </italic>(<italic>Chishao</italic>), which accounted for 95.21% of all detectable components [##UREF##28##52##]. In another study, 69 components of <italic>Radix Rehmanniae Preparata </italic>(<italic>Shudihuang</italic>) were separated, of which 59 were identified using standard spectra in the database of the National Institute of Standards and Technology (NIST). The 26 identified methyl esters accounted for about 94.29% of the total number of components [##UREF##29##53##]. Most of the iterative methods including (orthogonal projection approach) OPA and (iterative orthogonal projection resolution) IOP were applied to determine the chemical composition of herbal medicines [##REF##11206787##45##,##REF##14601831##46##]. With these chemometric methods, 65 volatile chemical constituents of <italic>Rhizoma et Radix Notopterygii </italic>(<italic>Qianghuo</italic>) were identified out of the 98 separated chemicals. Qi <italic>et al</italic>. resolved the overlapping chromatographic peaks in <italic>Resina Draconis </italic>(<italic>Xuejie</italic>) using HPLC-DAD. Therefore, using chemometric methods with hyphenated instruments was powerful in the analysis of herbal medicines [##UREF##30##54##]. Zeng <italic>et al</italic>. used PCA and generalized rank annihilation factor analysis (GRAFA) to process HPLC-DAD data sets obtained from <italic>Radix Salviae Miltiorrhizae </italic>(<italic>Danshen</italic>) and <italic>Radix Notoginseng </italic>(<italic>Sanqi</italic>) [##UREF##31##55##]. Ye <italic>et al</italic>. simultaneously analyzed seven major saponins of <italic>Danshen Diwan </italic>with HPLC-DAD and electron spray ionization-mass spectrometry (ESI-MS) [##UREF##32##56##].</p> <p>Chemometric methods including spectral correlative chromatography (SCC), multi-component spectral correlative chromatography (MSCC), AMWFA were proposed for comparing three-dimensional (3D) or two-dimensional (2D) chromatographic profiles and integrating presence or absence information [##REF##16430907##49##,##UREF##33##57##, ####REF##15612753##58##, ##UREF##34##59####34##59##]. SCC was used to compare pure or selective herbal medicine components [##UREF##34##59##]. Both MSCC and AMWFA were used to analyze complex herbal medicines. The main feature of MSCC is the construction of an orthogonal projection matrix using abstract spectra acquired from decomposition of original fingerprint data sets. For comparison, the pure and mixed spectra are projected to the matrix for presence or absence information of target components. AMWFA was used to extract pure chromatographic and spectral profiles of common components of related herbal medicines [##REF##16430907##49##,##REF##15612753##58##,##UREF##34##59##]. All these new chemometric algorithms were applied in the identification, quantification, comparison of chemical components and quality control of herbal medicines [##REF##14658022##60##, ####REF##15335044##61##, ##UREF##35##62##, ##UREF##36##63##, ##REF##17606017##64##, ##UREF##37##65##, ##UREF##38##66####38##66##].</p> <title>Pattern-oriented approach</title> <title>Single pattern approach</title> <title>Fingerprint analysis</title> <p>The pattern-oriented approach analyzes fingerprints obtained from one-, two- or higher dimensional chromatographic and/or spectral instruments. Single pattern approach focuses on one type of pattern (e.g. chemical fingerprints of chromatograms and spectra) for the quality control of herbal medicines. Significant progress was made in 2D chromatographic profiles obtained from HPLC, CE, GC as well as 3D ones from hyphenated instruments [##UREF##9##18##,##UREF##10##19##,##UREF##39##67##, ####REF##16999970##68##, ##UREF##40##69##, ##REF##17161980##70####17161980##70##]. Yan <italic>et al</italic>. combined 3D fingerprints from HPLC-DAD instruments with principal component analysis (PCA) to monitor <italic>Qingkailing </italic>(a proprietary Chinese medicine injection formulation) produced by various manufacturers [##REF##16196137##71##]. High-speed counter-current chromatography (HSCCC) and high-performance liquid chromatography-coulometric electrode array detector (HPLC-CEAD) were used to obtain the fingerprint of <italic>Radix Salviae Miltiorrhizae </italic>(<italic>Danshen</italic>) collected from various localities [##REF##14753780##72##, ####REF##15584232##73##, ##UREF##41##74####41##74##]. Binary chromatographic fingerprints from HPLC-DAD and GC-MS were used to analyze the aporphinoid and quinolizidine alkaloids of <italic>Caulophyllum robustum </italic>(<italic>Leiyemudan</italic>). Similarity index and the cluster analysis method were used to analyze the quality of ten batches of samples of <italic>Caulophyllum robustum </italic>[##UREF##42##75##]. Fan <italic>et al</italic>. developed multiple chromatographic fingerprinting including two HPLC fingerprints for the quality assessment of <italic>Danshen Diwan </italic>[##UREF##43##76##]. Thin layer chromatography scan (TLCS) provided unique fingerprints which differentiated passiflora and other herbs grown under various conditions [##UREF##11##21##,##UREF##44##77##]. Fourier transform infrared spectroscopy (FT-IR), NIR and two-dimensional correlation infrared spectroscopy (2D-IR) spectroscopy were used to construct spectral fingerprints of complex herbal medicines [##REF##15620519##20##,##REF##17512816##28##,##UREF##45##78##,##UREF##46##79##]. New fingerprinting techniques have recently emerged, such as oscillating fingerprints [##UREF##47##80##], electrochemistry fingerprints [##UREF##48##81##,##UREF##49##82##], X-ray diffraction (XRD) second derivative fingerprints [##UREF##50##83##] and high performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MSn) [##UREF##51##84##].</p> <title>Chemometric methods for fingerprint analysis</title> <p>It is important to extract useful information from chromatographic/spectral fingerprints which contain chemical information of all detectable components of herbal medicines. Chau <italic>et al</italic>. developed a software package Computer Aided Similarity Evaluation (CASE) for data processing under Matlab [##UREF##52##85##]. Gong <italic>et al</italic>. developed a series of chemometric methods to extract information from fingerprints [##REF##15032363##24##,##REF##12885076##86##,##REF##17723488##87##]. Xu <italic>et al</italic>. used target peak alignment (TPA) to correct the retention time shifts and multiplicative scattering correction (MSC) for response correction [##REF##16962124##88##]. In addition, wavelet technique was used for data pre-processing of fingerprints [##UREF##53##89##]. Comparison, assessment and discrimination analysis are key steps to process the 'standardized' fingerprints of herbal medicines. Chemometric methods are used to compare data among herbs with hundreds or even thousands of chemical components. This information can be further used to identify the origins of herbs, or to authenticate herbs. For example, research has made significant progress since the introduction of the similarity index and pattern recognition analysis [##UREF##54##90##, ####UREF##55##91##, ##UREF##56##92##, ##UREF##57##93##, ##REF##15909545##94####15909545##94##]. Information fusion and determination of relative entropy critical value were proposed for the similarity analysis of fingerprints of herbal medicines [##UREF##54##90##, ####UREF##55##91##, ##UREF##56##92##, ##UREF##57##93####57##93##]. PCA, orthogonal projection technique (OP), 2D-IR worked well on data discrimination analysis of fingerprints [##REF##15909545##94##, ####REF##16622674##95##, ##REF##17386812##96##, ##UREF##58##97####58##97##]. Data deconvolution methods helped obtain chromatographic and spectral profiles of individual pure components [##REF##15137973##47##, ####UREF##27##48##, ##REF##16430907##49##, ##REF##15478995##50##, ##REF##11269523##51##, ##UREF##28##52##, ##UREF##29##53####29##53##]. Furthermore, chemical fingerprint databases of herbal medicines were developed according to chemometric methods [##UREF##59##98##,##UREF##60##99##].</p> <title>Multi-pattern approach</title> <p>The multi-pattern approach assesses the quality of herbal medicines according to multiple patterns. For example, both chromatographic fingerprints and biological activity profiles are used for the quality control of herbal medicines. This approach targets the discovery of bioactive ingredients, assessment of medical effects, correlation between chemical fingerprints and pharmacological indices, and quality control [##REF##16132135##100##, ####REF##16496683##101##, ##REF##16213118##102##, ##UREF##61##103##, ##REF##17276058##104##, ##UREF##62##105####62##105##]. Some studies used chromatographic fingerprints from liquid chromatography-diode array detection-atmospheric pressure chemical ionization-mass spectroscopy (LC-DAD-APCI-MS) followed by data processing. Wang <italic>et al</italic>. found 32 potential bioactive components of <italic>Radix Angelicae Sinensis </italic>(<italic>Danggui</italic>) in rabbit plasma and over ten new compounds [##REF##16132135##100##]. Using a <italic>Herba Houttuyniae </italic>(<italic>Yuxingcao</italic>) injection, Lu <italic>et al</italic>. evaluated its anti-inflammatory effects and established the correlation between the chemical components of the injection and the bioactivities of the active ingredients [##REF##16496683##101##,##REF##16213118##102##]. Wang <italic>et al</italic>. devised a method which combined metabolic profiling and liquid chromatography-diode array detection-mass spectroscopy (LC/DAD-MS) with chemometrics to study <italic>Danggui Buxue Tang</italic>, a Chinese medicine formulation [##UREF##61##103##]. Aided by PCA, Yu <italic>et al</italic>. discovered major bioactive components of <italic>Aquilegia oxysepala </italic>(<italic>Jianeloudoucai</italic>) [##REF##17276058##104##]. Evidently, the multi-pattern approach provides a reliable means for the quality control of herbal medicines.</p>
<title>Chemometric methods for fingerprint analysis</title> <p>It is important to extract useful information from chromatographic/spectral fingerprints which contain chemical information of all detectable components of herbal medicines. Chau <italic>et al</italic>. developed a software package Computer Aided Similarity Evaluation (CASE) for data processing under Matlab [##UREF##52##85##]. Gong <italic>et al</italic>. developed a series of chemometric methods to extract information from fingerprints [##REF##15032363##24##,##REF##12885076##86##,##REF##17723488##87##]. Xu <italic>et al</italic>. used target peak alignment (TPA) to correct the retention time shifts and multiplicative scattering correction (MSC) for response correction [##REF##16962124##88##]. In addition, wavelet technique was used for data pre-processing of fingerprints [##UREF##53##89##]. Comparison, assessment and discrimination analysis are key steps to process the 'standardized' fingerprints of herbal medicines. Chemometric methods are used to compare data among herbs with hundreds or even thousands of chemical components. This information can be further used to identify the origins of herbs, or to authenticate herbs. For example, research has made significant progress since the introduction of the similarity index and pattern recognition analysis [##UREF##54##90##, ####UREF##55##91##, ##UREF##56##92##, ##UREF##57##93##, ##REF##15909545##94####15909545##94##]. Information fusion and determination of relative entropy critical value were proposed for the similarity analysis of fingerprints of herbal medicines [##UREF##54##90##, ####UREF##55##91##, ##UREF##56##92##, ##UREF##57##93####57##93##]. PCA, orthogonal projection technique (OP), 2D-IR worked well on data discrimination analysis of fingerprints [##REF##15909545##94##, ####REF##16622674##95##, ##REF##17386812##96##, ##UREF##58##97####58##97##]. Data deconvolution methods helped obtain chromatographic and spectral profiles of individual pure components [##REF##15137973##47##, ####UREF##27##48##, ##REF##16430907##49##, ##REF##15478995##50##, ##REF##11269523##51##, ##UREF##28##52##, ##UREF##29##53####29##53##]. Furthermore, chemical fingerprint databases of herbal medicines were developed according to chemometric methods [##UREF##59##98##,##UREF##60##99##].</p>
<title>Conclusion</title> <p>The compound-oriented and pattern-oriented approaches to the quality control of herbal medicines have been significantly improved in terms of analytical instruments, biological screening methods and chemometrics. Among all the advanced techniques, multi-pattern approach will have a great potential for further development.</p>
<p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0"/>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p> <p>The current approaches to the quality control of herbal medicines are either compound-oriented or pattern-oriented, the former targeting specific components with some known chemical properties and the latter targeting all detectable components. The marker approach uses specific chemical compounds with known molecular structures, while the multi-compound approach uses both chemical compounds with known structures and those with partial chemical information e.g. retention times, mass spectra and ultraviolet spectra. Apart from chromatographic techniques, new techniques such as oscillating and electrochemistry fingerprints have been developed for quality control. Chemometric resolution methods are widely used for component deconvolution and data comparison. Pattern recognition techniques are used for authentication of herbal medicines.</p>
<title>Abbreviations</title> <p>2D-IR: two-dimensional correlation infrared spectroscopy; AMWFA: alternative moving window factor analysis; CE: capillary electrophoresis; CRM: chemometric resolution methods; CZE: capillary zone electrophoresis; DAD: diode array detection; ESI-MS: electron spray ionization-mass spectrometry; EWOP: evolving window orthogonal projection; FSMWEFA: fixed-size moving window evolving factor analysis; FT-IR: Fourier transform infrared spectroscopy; GC: gas chromatography; GC-MS: gas chromatography-mass spectroscopy; GRAFA: generalized rank annihilation factor analysis; HELP: heuristic evolving latent projection; HPLC: High-performance liquid chromatography; HPLC-CEAD: high-performance liquid chromatography-coulometric electrode array detector; HPLC-DAD: high-performance liquid chromatography-diode array detection; HPLC-ESI-MSn: high-performance liquid chromatography electrospray ionization tandem mass spectrometry; HSCCC: high-speed counter-current chromatography; IOP: iterative orthogonal projection resolution; LC-DAD-APCI-MS: liquid chromatography-diode array detection-atmospheric pressure chemical ionization-mass spectroscopy; LC/DAD-MS: liquid chromatography-diode array detection-mass spectroscopy; LC-MS: liquid chromatography-mass spectroscopy; LC-MS/MS: liquid chromatography-tandem mass spectrometers; MS: mass spectroscopy, MSCC: multi-component spectral correlative chromatography; NIR: near infrared spectroscopy, OP: orthogonal projection technique; OPA: orthogonal projection approach, PCA: principle component analysis; PLS: partial least squares; SCC: spectral correlative chromatography; SFA: subwindow factor analysis, TLC: thin layer chromatography; TLC-UV: thin layer chromatography-ultraviolet spectrophotometry; XRD: X-ray diffraction.</p> <title>Competing interests</title> <p>The authors declare that they have no competing interests.</p> <title>Authors' contributions</title> <p>ZZ and FC conceived the classification of approaches to quality control of herbal medicines. ZZ drafted the manuscript. FC supervised the project and revised the manuscript. HC and CYC provided references and helped revise the manuscript. TL and SW advised on the manuscript. DM, COC and YL were responsible for some studies in this work. All authors read and approved the final manuscript.</p>
<title>Acknowledgements</title> <p>This work was financially supported by the University Grants Committee of the Hong Kong SAR CMRFD project (A0E/B-10/01), Innovation and Technology Committee of the Hong Kong SAR ITF-TCFS project (GHP/037/05), Taskforce for Development of the Hong Kong Polytechnic University BB8H and BB6R projects, National Nature Foundation Committee of China (20475066 and 10771217) and International Cooperative Project for Traditional Chinese Medicine of the Ministry of Science and Technology of China (2006DFA41090 and 2007DFA40680).</p>
[{"surname": ["Liang", "Kvalheim", "Manne"], "given-names": ["YZ", "OM", "R"], "article-title": ["White, gray and black multicomponent systems \u2013 a classification of mixture problems and methods for their quantitative analysis"], "source": ["Chemom Intell Lab Syst"], "year": ["1993"], "volume": ["18"], "fpage": ["235"], "lpage": ["250"], "pub-id": ["10.1016/0169-7439(93)85001-W"]}, {"surname": ["Mok", "Chau"], "given-names": ["DKW", "FT"], "article-title": ["Chemical information of Chinese medicines: A challenge to chemist"], "source": ["Chemom Intell Lab Syst"], "year": ["2006"], "volume": ["82"], "fpage": ["210"], "lpage": ["217"], "pub-id": ["10.1016/j.chemolab.2005.05.006"]}, {"surname": ["Yi", "Yu", "Liang", "Zeng"], "given-names": ["ZB", "Y", "YZ", "B"], "article-title": ["Short communication Evaluation of the antimicrobial mode of berberine by LC/ESI-MS combined with principal component analysis"], "source": ["J Pharmaceut Biomed"], "year": ["2007"], "volume": ["44"], "fpage": ["301"], "lpage": ["304"], "pub-id": ["10.1016/j.jpba.2007.02.018"]}, {"collab": ["The State Pharmacopoeia Commission of People's Republic of China"], "source": ["Pharmacopoeia of the People's Republic of China"], "year": ["2005"], "publisher-name": ["Beijing: Chemical Industry Press"]}, {"surname": ["Liang", "Xie", "Chan"], "given-names": ["YZ", "PS", "K"], "article-title": ["Quality control of herbal medicines"], "source": ["J Chromatogr B"], "year": ["2004"], "volume": ["812"], "fpage": ["53"], "lpage": ["70"]}, {"surname": ["Dra\u0161ar", "Moravcova"], "given-names": ["P", "J"], "article-title": ["Recent advances in analysis of Chinese medical plants and traditional medicines"], "source": ["J Chromatogr B"], "year": ["2004"], "volume": ["812"], "fpage": ["3"], "lpage": ["21"]}, {"surname": ["Xie"], "given-names": ["PS"], "article-title": ["Discussion of the present situations, development and problems of fingerprint of Chinese medicines"], "source": ["Zhongyaocai"], "year": ["2007"], "volume": ["3"], "fpage": ["257"], "lpage": ["259"]}, {"surname": ["Niu", "Niu", "Xie", "Fang", "Zhang", "Cui", "Tu"], "given-names": ["F", "ZQ", "GB", "M", "GE", "Z", "PF"], "article-title": ["Development of an HPLC fingerprint for quality control of Radix Semiaquilegiae"], "source": ["Chromatographia"], "year": ["2006"], "volume": ["64"], "fpage": ["593"], "lpage": ["597"], "pub-id": ["10.1365/s10337-006-0069-6"]}, {"surname": ["Xie", "Qiu", "Zhao", "Jia"], "given-names": ["GX", "MF", "AH", "W"], "article-title": ["Fingerprint analysis of Flos Carthami by pressurized CEC and LC"], "source": ["Chromatographia"], "year": ["2006"], "volume": ["64"], "fpage": ["739"], "lpage": ["743"], "pub-id": ["10.1365/s10337-006-0083-8"]}, {"surname": ["Sun", "Guo", "Sui", "Li"], "given-names": ["Y", "T", "Y", "FM"], "article-title": ["Fingerprint analysis of "], "italic": ["Flos Carthami "], "source": ["J Chromatogr B"], "year": ["2003"], "volume": ["792"], "fpage": ["147"], "lpage": ["152"], "pub-id": ["10.1016/S1570-0232(03)00255-1"]}, {"surname": ["Zhao", "Huang", "Shan", "Xiang", "Mei"], "given-names": ["LH", "CY", "Z", "BR", "LH"], "article-title": ["Fingerprint analysis of "], "italic": ["Psoralea corylifolia "], "source": ["J Chromatogr B"], "year": ["2005"], "volume": ["821"], "fpage": ["67"], "lpage": ["74"], "pub-id": ["10.1016/j.jchromb.2005.04.008"]}, {"surname": ["Birk", "Provensi", "Gosmann"], "given-names": ["CD", "G", "G"], "article-title": ["TLC fingerprint of flavonoids and saponins from passiflora species"], "source": ["J Liq Chromatogr R T"], "year": ["2005"], "volume": ["8"], "fpage": ["2285"], "lpage": ["2291"], "pub-id": ["10.1081/JLC-200064212"]}, {"surname": ["Li", "Hu", "Liang", "Xie", "Du"], "given-names": ["BY", "Y", "YZ", "PS", "YP"], "article-title": ["Quality evaluation of fingerprints of herbal medicine with chromatographic data"], "source": ["Anal Chim Acta"], "year": ["2004"], "volume": ["514"], "fpage": ["69"], "lpage": ["77"], "pub-id": ["10.1016/j.aca.2004.03.041"]}, {"surname": ["Liu", "Sun", "Lv", "Liang"], "given-names": ["HX", "SQ", "GH", "XY"], "article-title": ["Discrimination of extracted lipophilic constituents of Angelica with multi-steps infrared macro-fingerprint method"], "source": ["Vib Spectrosc"], "year": ["2006"], "volume": ["40"], "fpage": ["202"], "lpage": ["208"], "pub-id": ["10.1016/j.vibspec.2005.09.004"]}, {"surname": ["Xu", "Liang", "Chau"], "given-names": ["CJ", "YZ", "FT"], "article-title": ["Identification of essential components of "], "italic": ["Houttuynia cordata "], "source": ["Talanta"], "year": ["2005"], "volume": ["68"], "fpage": ["108"], "lpage": ["115"], "pub-id": ["10.1016/j.talanta.2005.04.043"]}, {"surname": ["Cai", "Zhou", "Gesang", "Bianba", "Ding"], "given-names": ["M", "Y", "SL", "C", "LS"], "article-title": ["Chemical fingerprint analysis of rhizomes of "], "italic": ["Gymnadenia conopsea ", "n"], "source": ["J Chromatogr B"], "year": ["2006"], "volume": ["844"], "fpage": ["301"], "lpage": ["307"], "pub-id": ["10.1016/j.jchromb.2006.07.043"]}, {"surname": ["Xie", "Jiang", "Zhou", "Cai", "Wong", "Liu", "Bian", "Xu", "Liu"], "given-names": ["Y", "ZH", "H", "X", "Yuen-F", "ZQ", "ZX", "HX", "L"], "article-title": ["Combinative method using HPLC quantitative and qualitative analyses for quality consistency assessment of a herbal medicinal preparation"], "source": ["J Pharmaceut Biomed"], "year": ["2007"], "volume": ["43"], "fpage": ["204"], "lpage": ["212"], "pub-id": ["10.1016/j.jpba.2006.07.008"]}, {"surname": ["Lin", "Qiu", "Wufuer", "Shum"], "given-names": ["ZPJ", "SX", "A", "L"], "article-title": ["Simultaneous determination of glycyrrhizin, a marker component in radix Glycyrrhizae, and its major metabolite glycyrrhetic acid in human plasma by LC-MS/MS"], "source": ["J Chromatogr B"], "year": ["2005"], "volume": ["814"], "fpage": ["201"], "lpage": ["207"], "pub-id": ["10.1016/j.jchromb.2004.10.026"]}, {"surname": ["Huang", "Liang", "Guo", "Zhou", "Chen"], "given-names": ["LF", "YZ", "FQ", "ZF", "BM"], "article-title": ["Short communication Simultaneous separation and determination of active components in Cordyceps sinensis and Cordyceps militarris by LC/ESI-MS"], "source": ["J Pharmaceut Biomed"], "year": ["2003"], "volume": ["33"], "fpage": ["1155"], "lpage": ["1162"], "pub-id": ["10.1016/S0731-7085(03)00415-1"]}, {"surname": ["Li", "Jiang", "Guan", "Liu", "Zhou", "Li", "Huang", "Sun", "Peng", "Zhou"], "given-names": ["YH", "SY", "YL", "X", "Y", "LM", "SX", "HD", "SL", "Y"], "article-title": ["Quantitative determination of the chemical profile of the plant material \"Qiang-huo\" by LC-ESI-MS-MS"], "source": ["Chromatographia"], "year": ["2006"], "volume": ["64"], "fpage": ["405"], "lpage": ["411"], "pub-id": ["10.1365/s10337-006-0052-2"]}, {"surname": ["Wang"], "given-names": ["CH"], "article-title": ["GC-MS analysis of aconitine in biological tests"], "source": ["Zhongguo Fayixue Zazhi"], "year": ["2003"], "volume": ["3"], "fpage": ["145"], "lpage": ["146"]}, {"surname": ["Sun", "Dai", "Chang", "Yang"], "given-names": ["XM", "DM", "XL", "XL"], "article-title": ["Simultaneous determination of camphor, menthol, synthetic borneol and methyl salicylate in Shexiang Zhuanggu Plaster by GC"], "source": ["Zhongchengyao"], "year": ["2007"], "volume": ["7"], "fpage": ["1004"], "lpage": ["1008"]}, {"surname": ["Yu", "Gong", "Lin", "Cheng"], "given-names": ["K", "YF", "ZY", "YY"], "article-title": ["Quantitative analysis and chromatographic fingerprinting for the quality evaluation of Scutellaria baicalensis Georgi using capillary electrophoresis"], "source": ["J Pharmaceut Biomed"], "year": ["2007"], "volume": ["43"], "fpage": ["540"], "lpage": ["548"], "pub-id": ["10.1016/j.jpba.2006.08.011"]}, {"surname": ["Tong", "Li", "Pan"], "given-names": ["JY", "W", "CM"], "article-title": ["TLC identification and total flavonoids determination in herb sarcandra from different producing areas"], "source": ["Zhongyao Xinyao Yu Linchuang Yaoli"], "year": ["2007"], "volume": ["4"], "fpage": ["105"], "lpage": ["107"]}, {"surname": ["Liu", "Ao", "Song"], "given-names": ["L", "L", "J"], "article-title": ["Determination of daphnetin in girald daphne bark fumigant by TCL-UV spectrophotometry"], "source": ["Zhongguo Yaofang"], "year": ["2007"], "volume": ["15"], "fpage": ["1172"], "lpage": ["1173"]}, {"surname": ["Yan", "Xin", "Luo", "Wang", "Chen"], "given-names": ["SK", "WF", "GA", "YM", "YY"], "article-title": ["Simultaneous determination of five groups of componentsin Qingkailing injection by high performance liquidchromatography with photo diode array detectorand evaporative light scattering detector"], "source": ["Sepu"], "year": ["2005"], "volume": ["5"], "fpage": ["482"], "lpage": ["486"]}, {"surname": ["Ohtakea", "Nakaib", "Yamamotoa", "Sakakibarab", "Takedaa", "Amagayab", "Aburadac"], "given-names": ["N", "Y", "M", "I", "S", "S", "M"], "article-title": ["Review Separation and isolation methods for analysis of the active principles of Sho-saiko-to (SST) oriental medicine"], "source": ["J Chromatogr B"], "year": ["2004"], "volume": ["812"], "fpage": ["135"], "lpage": ["148"]}, {"surname": ["Zhao", "Li", "Liang", "Fang", "Huang", "Guo"], "given-names": ["CX", "XN", "YZ", "HZ", "LF", "FQ"], "article-title": ["Comparative analysis of chemical components of essential oils from different samples of Rhododendron with the help of chemometrics methods"], "source": ["Chemom Intell Lab Syst"], "year": ["2006"], "volume": ["82"], "fpage": ["218"], "lpage": ["228"], "pub-id": ["10.1016/j.chemolab.2005.08.008"]}, {"surname": ["Li", "Lan", "Liang"], "given-names": ["XR", "ZG", "YZ"], "article-title": ["Analysis of volatile chemical components of Radix Paeoniae Rubra by gas chromatography mass spectrometry and chemometric resolution"], "source": ["Zhongnan Gongye Daxue Xuebao"], "year": ["2007"], "volume": ["14"], "fpage": ["57"], "lpage": ["61"]}, {"surname": ["Li", "Liang", "Du", "Xu", "Li", "Song", "Cui"], "given-names": ["BY", "YZ", "YP", "CJ", "XN", "YQ", "H"], "article-title": ["Resolution and identification of the acidic fraction of a petroleum ether extract of "], "italic": ["Radix Rehmanniae Preparata "], "source": ["Chromatographia"], "year": ["2003"], "volume": ["57"], "fpage": ["235"], "lpage": ["243"], "pub-id": ["10.1007/BF02491722"]}, {"surname": ["Li", "Liang", "Xie", "Yu"], "given-names": ["BY", "YZ", "PS", "RQ"], "article-title": ["Spectral correlative chromatography and its application to the analysis of characteristic chemical fingerprints of traditional Chinese medicines"], "source": ["Fenxi Huaxue"], "year": ["2003"], "volume": ["07"], "fpage": ["799"], "lpage": ["803"]}, {"surname": ["Qi", "Sun", "Wu", "Li", "Mi", "Chai"], "given-names": ["YP", "SL", "YT", "TH", "HM", "YF"], "article-title": ["Application of heuristic evolving latent projection to the determination of loureirin B in resina draconis extracted by different methods"], "source": ["Fenxi Huaxue"], "year": ["2002"], "volume": ["30"], "fpage": ["797"], "lpage": ["800"]}, {"surname": ["Zeng", "Cheng", "Shen"], "given-names": ["Z", "YY", "GF"], "article-title": ["A computational method for quantitative determining the active component of chinese medicine integrating PCA with GRAFA"], "source": ["Acta Chimica Sinica"], "year": ["2003"], "volume": ["61"], "fpage": ["84"], "lpage": ["88"]}, {"surname": ["Makris", "Kallithraka", "Mamalos"], "given-names": ["DP", "S", "A"], "article-title": ["Differentiation of young red wines based on cultivar and geographical origin with application of chemometrics of principal polyphenolic constituents"], "source": ["Talanta"], "volume": ["70"], "fpage": ["1143"], "lpage": ["1152"], "pub-id": ["10.1016/j.talanta.2006.03.024"]}, {"surname": ["Hu", "Liang", "Li Bo", "Xu", "Zeng"], "given-names": ["Y", "YZ", "Y", "CJ", "ZD"], "article-title": ["Multicomponent spectral correlative chromatography applied to analysis of chemical fingerprints of traditional Chinese medicines"], "source": ["Acta Chim Sinica"], "year": ["2003"], "volume": ["9"], "fpage": ["1466"], "lpage": ["1470"]}, {"surname": ["Wang", "Liang", "Hu", "Li", "Zeng", "Xu"], "given-names": ["YL", "YZ", "Y", "BY", "ZD", "SP"], "article-title": ["Comparison studies of constituents between dangguibuxuetang and its single medicines via hyphenated chromatography and multicomponent spectral correlative chromatography"], "source": ["Gaodeng Xuexiao Huaxue Xuebao"], "year": ["2006"], "volume": ["2"], "fpage": ["222"], "lpage": ["226"]}, {"surname": ["Wang", "Liang", "Hu", "Li", "Zeng", "He"], "given-names": ["YL", "YZ", "Y", "BY", "ZD", "YK"], "article-title": ["Study on absorption of Angelica sinensis in rabbit plasma by HPLC-DAD-MS and multicomponent spectral correlative chromatography"], "source": ["Chemom Intell Lab Syst"], "year": ["2006"], "volume": ["82"], "fpage": ["229"], "lpage": ["235"], "pub-id": ["10.1016/j.chemolab.2005.08.006"]}, {"surname": ["Yi", "Liang", "Zeng", "Yuan", "Wang"], "given-names": ["LZ", "YZ", "ZD", "DL", "P"], "article-title": ["AMWFA method applied to comparative analysis of two-dimensional data with overlapped peaks"], "source": ["Gaodeng Xuexiao Huaxue Xuebao"], "year": ["2006"], "volume": ["11"], "fpage": ["2052"], "lpage": ["2055"]}, {"surname": ["Yi", "Liang", "Zeng", "Wang", "Yuan"], "given-names": ["LZ", "YZ", "ZD", "P", "DL"], "article-title": ["Comparative Analysis of Volatile Constituents in Chenpi of Different Original Plants by GC-MS and AMWFA"], "source": ["Gaodeng Xuexiao Huaxue Xuebao"], "year": ["2006"], "volume": ["09"], "fpage": ["1626"], "lpage": ["1630"]}, {"surname": ["Cao", "Wang", "Yu", "Ye"], "given-names": ["YH", "LC", "XJ", "JN"], "article-title": ["Development of the chromatographic fingerprint of herbal preparations Shuang-Huang-Lian oral liquid"], "source": ["J Pharmaceut Biomed"], "year": ["2006"], "volume": ["41"], "fpage": ["845"], "lpage": ["856"], "pub-id": ["10.1016/j.jpba.2006.01.060"]}, {"surname": ["Fan", "Wang", "Cheng"], "given-names": ["XH", "Y", "YY"], "article-title": ["LC/MS fingerprinting of "], "italic": ["Shenmai injection"], "source": ["J Pharmaceut Biomed"], "year": ["2006"], "volume": ["40"], "fpage": ["591"], "lpage": ["597"], "pub-id": ["10.1016/j.jpba.2005.10.036"]}, {"surname": ["Ma", "Zhang", "Zhang", "Gan"], "given-names": ["LJ", "XZ", "HP", "YR"], "article-title": ["Development of a fingerprint of "], "italic": ["Salvia miltiorrhiza Bunge "], "source": ["J Chromatogr B"], "year": ["2007"], "volume": ["846"], "fpage": ["139"], "lpage": ["146"], "pub-id": ["10.1016/j.jchromb.2006.08.026"]}, {"surname": ["Li", "Hu", "He"], "given-names": ["YP", "Z", "LC"], "article-title": ["An approach to develop binary chromatographic fingerprints of the total alkaloids from Caulophyllum robustum by high performance liquid chromatography/diode array detector and gas chromatography/mass spectrometry"], "source": ["J Pharmaceut Biomed"], "year": ["2007"], "volume": ["43"], "fpage": ["1667"], "lpage": ["1672"], "pub-id": ["10.1016/j.jpba.2006.12.028"]}, {"surname": ["Fan", "Cheng", "Ye", "Lin", "Qian"], "given-names": ["XH", "YY", "ZL", "RC", "ZZ"], "article-title": ["Multiple chromatographic fingerprinting and its application to the quality control of herbal medicines"], "source": ["Anal Chim Acta"], "year": ["2006"], "volume": ["555"], "fpage": ["217"], "lpage": ["224"], "pub-id": ["10.1016/j.aca.2005.09.037"]}, {"surname": ["Gu", "Su", "Ouyang"], "given-names": ["M", "ZG", "F"], "article-title": ["Fingerprinting of salvia miltiorrhiza bunge by thin-layer chromatography scan compared with high speed countercurrent chromatography"], "source": ["J Liquid Chromatogr R T"], "year": ["2006"], "volume": ["29"], "fpage": ["1503"], "lpage": ["1514"], "pub-id": ["10.1080/10826070600674968"]}, {"surname": ["Liu", "Sun", "Lv", "Chan"], "given-names": ["HX", "SQ", "GH", "KKC"], "article-title": ["Study on "], "italic": ["Angelica "], "source": ["Spectrochim Acta A"], "year": ["2006"], "volume": ["64"], "fpage": ["321"], "lpage": ["326"], "pub-id": ["10.1016/j.saa.2005.07.026"]}, {"surname": ["Zou", "Yang", "Qin", "Jiang", "Du", "Aboul-Enein"], "given-names": ["HB", "GS", "ZR", "WQ", "AQ", "HY"], "article-title": ["Progress in quality control of herbal medicine with IR fingerprint spectra"], "source": ["Anal Lett"], "year": ["2005"], "volume": ["38"], "fpage": ["1457"], "lpage": ["1475"], "pub-id": ["10.1081/AL-200062153"]}, {"surname": ["Li", "Yuan", "Nie"], "given-names": ["ZX", "CL", "F"], "article-title": ["Application of chemical oscillation technique in herb's study"], "source": ["Zhongguo Jichu Kexue"], "year": ["2004"], "volume": ["2"], "fpage": ["27"], "lpage": ["29"]}, {"surname": ["Zhang", "Liang", "Yuan", "Ding", "Zhang", "Wei", "Chen"], "given-names": ["TM", "YZ", "B", "F", "YP", "MQ", "X"], "article-title": ["Testing methods, conditions and factors of electrochemistry fingerprints of herbal medicines"], "source": ["Kexue Tongbao"], "year": ["2007"], "volume": ["9"], "fpage": ["1012"], "lpage": ["1020"]}, {"surname": ["Zhang", "Liang", "Yuan", "Ding", "Zhang", "Chen"], "given-names": ["TM", "YZ", "B", "F", "YP", "WY"], "article-title": ["Principles, features and applications of electrochemistry fingerprints of herbal medicines"], "source": ["Kexue Tongbao"], "year": ["2007"], "volume": ["13"], "fpage": ["1513"], "lpage": ["1522"]}, {"surname": ["Yang", "Hu", "Guo", "Li"], "given-names": ["JH", "EP", "LH", "H"], "article-title": ["Methodology of XRD second derivative fingerprint for traditional Chinese medicine"], "source": ["Nat Prod Res Dev"], "year": ["2006"], "volume": ["18"], "fpage": ["390"], "lpage": ["393"]}, {"surname": ["Ran", "Yang", "Liang", "Chen", "Wang", "Luo", "Li", "Li", "Chen"], "given-names": ["XR", "HH", "QL", "J", "YM", "GA", "P", "KM", "YW"], "article-title": ["Difference analysis of mass spectra and its application to research of Chinese multiherb remedy"], "source": ["Gaodeng Xuexiao Huaxue Yanjiu"], "year": ["2007"], "volume": ["2"], "fpage": ["250"], "lpage": ["253"]}, {"surname": ["Gong", "Wang", "Chau", "Liang"], "given-names": ["F", "BT", "FT", "YZ"], "article-title": ["HPLC Data preprocessing for chromatographic fingerprint of herbal medicine with chemometric approaches"], "source": ["Anal Lett"], "year": ["2005"], "volume": ["38"], "fpage": ["2475"], "lpage": ["2492"], "pub-id": ["10.1080/00032710500318338"]}, {"surname": ["Yang", "Zhu"], "given-names": ["Y", "XF"], "article-title": ["Baseline spliting of traditional Chinese medicine fingerprint based on the second-generation wavelet transform"], "source": ["Huanan Ligong Daxue (Ziran Kexue Ban)"], "year": ["2007"], "volume": ["1"], "fpage": ["29"], "lpage": ["52"]}, {"surname": ["Fang", "Liang", "Yin", "Chan", "Lu"], "given-names": ["KT", "YZ", "XL", "K", "GH"], "article-title": ["Critical value determination on similarity of fingerprints"], "source": ["Chemom Intell Lab Syst"], "year": ["2006"], "volume": ["82"], "fpage": ["236"], "lpage": ["240"], "pub-id": ["10.1016/j.chemolab.2005.06.018"]}, {"surname": ["Fan", "Ye", "Cheng"], "given-names": ["XH", "ZL", "YY"], "article-title": ["A computational method based on information fusion for evaluating the similarity of multiple chromatographic fingerprints of TCM"], "source": ["Gaodeng Xuexiao Huaxue Yanjiu"], "year": ["2006"], "volume": ["1"], "fpage": ["26"], "lpage": ["29"]}, {"surname": ["Liu", "Cao", "Wang", "Luo"], "given-names": ["YS", "M", "YM", "GA"], "article-title": ["Similarity system theory to evaluate the similarity of high performance liquid chromatography fingerprint of traditional Chinese medicine quantitatively"], "source": ["Fenxi Huaxue"], "year": ["2006"], "volume": ["3"], "fpage": ["333"], "lpage": ["337"]}, {"surname": ["Wang", "Du", "Li"], "given-names": ["K", "K", "H"], "article-title": ["The similarity algorithm study on fingerprint of traditional Chinese medicine based on relative entropy"], "source": ["Comp Appl Chem"], "year": ["2007"], "volume": ["1"], "fpage": ["49"], "lpage": ["52"]}, {"surname": ["Liu", "Sun", "Lv", "Liang"], "given-names": ["HX", "SQ", "GH", "XY"], "article-title": ["Discrimination of extracted lipophilic constituents of Angelica with multi-steps infrared macro-fingerprint method"], "source": ["Vib Spectrosc"], "year": ["2006"], "volume": ["40"], "fpage": ["202"], "lpage": ["208"], "pub-id": ["10.1016/j.vibspec.2005.09.004"]}, {"surname": ["Chen", "Zeng", "Liang", "Du", "Yi"], "given-names": ["S", "ZD", "YZ", "YP", "LZ"], "article-title": ["Realization and application of fingerprint database system of Herba houttuyniae injection"], "source": ["Comp App Chem"], "year": ["2006"], "volume": ["9"], "fpage": ["835"], "lpage": ["838"]}, {"surname": ["Liu", "Yu", "Cai", "Qian"], "given-names": ["XH", "S", "BC", "JD"], "article-title": ["Comprehensive database of TCM fingerprint"], "source": ["Zhongchengyao"], "year": ["2005"], "volume": ["10"], "fpage": ["1117"], "lpage": ["1119"]}, {"surname": ["Wang", "Liang", "Zhou", "Chen", "Yi", "Yu", "Yi"], "given-names": ["P", "YZ", "N", "BM", "LZ", "Y", "ZB"], "article-title": ["Screening and analysis of the multiple absorbed bioactive components and metabolites of Dangguibuxue decoction by the metabolic fingerprinting technique and liquid chromatography/diode-array detection mass spectrometry"], "source": ["Rapid Commun Mass Sp"], "year": ["2007"], "volume": ["21"], "fpage": ["99"], "lpage": ["106"], "pub-id": ["10.1002/rcm.2816"]}, {"surname": ["Liang", "Xu"], "given-names": ["XM", "Q"], "article-title": ["Strategy and methodology for analysis of active component fingerprint of traditional Chinese medicines"], "source": ["Zhongguo Tianran Yaowu"], "year": ["2003"], "volume": ["1"], "fpage": ["57"], "lpage": ["60"]}]
Chin Med. 2008 Aug 4; 3:9