<p>This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p> <p>Methods that aim at predicting interaction partners are very likely to play an important
role in the interpretation of genomic information. iSPOT (iSpecificity Prediction Of
Target) is a web tool (accessible at http://cbm.bio.uniroma2.it/iSPOT) developed for the
prediction of protein-protein interaction mediated by families of peptide recognition
modules. iSPOT accesses a database of position specific residue-residue interaction
frequencies for members of the SH3 and PDZ protein domain families. The software
utilises this database to provide a score for any potential domain peptide interaction.</p> <p>iSPOT: 1. evaluates the likelihood of the interaction between any of the peptides
contained in an input protein and a list of domains of the two different families; 2. searches
in the SWISS-PROT database for potential partners of a query domain; and 3. has access
to a repository of all the domain/target peptide interaction data.</p>
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Comp Funct Genomics. 2001 Oct; 2(5):314-318