<p>This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p> <p>Aiming to discern the mechanisms by which circulating
glycated albumin alters the glomerular filtration properties
that lead to glomerular dysfunction in diabetes, the authors
studied the distribution and densities of anionic charges
through the rat glomerular wall upon intravascular infusion
of Amadori products, as well as in various conditions of
increased glomerular permselectivity. Polylysine-gold was
used as the probe to reveal the anionic charges. The study
was carried on renal tissue sections of bovine serum albumin
(BSA)- and glycated BSA–injected, normoglycemic
animals. Results were generated through morphometrical
evaluations of the gold labeling. Changes in glomerular anionic
distribution were corroborated on renal tissue sections
of short- and long-term diabetic rats and of normal newborn
rats, situations known for abnormal glomerular filtration.
Altered renal function in these conditions was clearly
associated with changes in glomerular anionic charges. On
the other hand, the infusion of glycated albumin in the circulation
of normal rats, though altering glomerular filtration
properties, did not modify the distribution and density of
the polylysine-gold labeling through the glomerular basement
membrane. Thus, anionic charges seem not to be the
factor involved in the early changes of glomerular permeability
induced by circulating glycated albumin.</p>
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Exp Diabesity Res. 2003; 4(2):83-92