<p>This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p> <p>Plasma levels of glucose, insulin and glucagon were
measured at various time intervals after pancreatic
duct ligation (PDL) in rabbits. Two hyperglycemic
periods were observed: one between 15–90 days
(peak at 30 days of 15.1 ± 1.2mmol/l, p &lt; 0.01), and
the other at 450 days (11.2 ± 0.5 mmol/l, p &lt; 0.02). The
first hyperglycemic episode was significantly correlated
with both hypoinsulinemia (41.8 ± 8pmol/l,
r= –0.94, p &lt; 0.01) and hyperglucagonemia (232 ± 
21ng/l, r=0.95, p &lt; 0.01). However, the late hyperglycemic
phase (450 days), which was not accompanied
by hypoinsulinemia, was observed after the
hyperglucagonemia (390 days) produced by abundant
immunostained A-cells giving rise to a 3-fold
increase in pancreatic glucagon stores. The insulin
and glucagon responses to glucose loading at 180,
270 and 450 days reflected the insensitivity of B- and
A-cells to glucose. The PDL rabbit model with
chronic and severe glycemic disorders due to the
predominant role of glucagon mimicked key features
of the NIDDM syndrome secondary to
exocrine disease.</p>
[]
Int J Exp Diabetes Res. 2001; 2(2):101-112