<p>This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p> <p>The tissues and organs of multicellular eukaryotes are frequently observed to
comprise complex three-dimensional interspersions of different cell types. It is
a reasonable assumption that different global patterns of gene expression are
found within these different cell types. This review outlines general experimental
strategies designed to characterize these global gene expression patterns, based on
a combination of methods of transgenic fluorescent protein (FP) expression and
targeting, of flow cytometry and sorting and of high-throughput gene expression
analysis.</p>
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Comp Funct Genomics. 2003 Apr; 4(2):208-215