What is sequencer when ion torrent pgm 200- bp?
Sequencer Ion Torrent PGM 454 GS FLX HiSeq 2000 SOLiDv4 PacBio Sanger 3730xl
DNA sequencer
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1-127511-1
Comparison
Comparison
Manufacturer Ion Torrent (Life Technologies) 454 Life Sciences (Roche) Illumina Applied Biosystems (Life Technologies) Pacific Biosciences Applied Biosystems (Life Technologies)
Sequencing Chemistry Ion semiconductor sequencing Pyrosequencing Polymerase-based sequence-by-synthesis Ligation-based sequencing Phospholinked fluorescent nucleotides Dideoxy chain termination
Amplification approach Emulsion PCR Emulsion PCR Bridge amplification Emulsion PCR Single-molecule; no amplification PCR
Data output per run 100-200 Mb 0.7 Gb 600 Gb 120 Gb 100-500 Mb 1.9∼84 Kb
Accuracy 99% 99.9% 99.9% 99.94% 90% 99.999%
Time per run 2 hours 24 hours 3–10 days 7–14 days 2 hours 20 minutes - 3 hours
Read length 200-400 bp 700 bp 100x100 bp paired end 50x50 bp paired end 1,500-5,000 bp (avg) 400-900 bp
Cost per run $350 USD $7,000 USD $6,000 USD (30x human genome) $4,000 USD $300 USD $4 USD (single read/reaction)
Cost per Mb $1.00 USD $10 USD $0.07 USD $0.13 USD $2.00 USD $2400 USD
table_127511_1
SELECT Sequencer FROM table WHERE Ion Torrent PGM = 200-400 bp
200-400 bp