Figure S3 from Selective Tumor Cell Apoptosis and Tumor Regression in CDH17-Positive Colorectal Cancer Models using BI 905711, a Novel Liver-Sparing TRAILR2 Agonist
B

17

R2 = 0.38; p<0.01

SW1463

LS513
NCI-H508

NCI-H716

SNU-C1

R2 = 0.65; p<0.001

LS1034

12 GP2d

SNU-C4

COLO 205
SW48

SW403

LoVo

CL-34

SK-CO-1
COLO 205

T84
C2BBe1

13
DLD-1
GEO

SNU-1033

11

LS 174T

DLD-1

11 CL-34

SNU-C4

SK-CO-1
SW1463

10

HCT-8

HCT-15
C2BBe1

GP5d
SNU-1033
SW403

LoVo
HCT-8
SNU-C1

9

SW48

LS 174T
NCI-H716
T84

SW837

9

GEO
SW837

HCT-15

0

25

50

75

0

25

BI 905711 minPOC (%)

C

50

75

BI 905711 minPOC (%)

17

SW1463
SNU-C4

NCI-H716

GP2d
NCI-H508

SNU-C1

LS1034

LS513

15
SW403

CDH17 protein level
(average log2 MFI)

CDH17 protein level
(average log2 MFI)

GP2d NCI-H508
LS1034
LS513
15 GP5d

TRAILR2 protein level
(average log2 MFI)

A

CL-34

GP5d
LoVo

C2BBe1 SK-CO-1 COLO 205

T84

13
DLD-1

11

sensitive
intermediate
insensitive

SNU-1033

GEO

LS 174T

HCT-8
SW48

SW837
HCT-15

9
9

10

11

12

TRAILR2 protein level
(average log2 MFI)

Supplementary Figure 3: CDH17 and TRAILR2 protein expression and their correlation with BI 905711 minPOC
in CRC cell lines. XY plot showing the correlation between CDH17(A) and TRAILR2(B) FACS measured protein
(average log2 MFI) and BI 905711 min POC in 24 CRC cell lines. A significant inverse correlation of both TRAILR2
and CDH17 protein expression with BI 905711 minPOC was detected. R squared and P value for the corresponding
Pearson correlation are shown. (C) Distribution of the 24 CRC cell lines according to their sensitivity to BI 905711
and with respect to CDH17 and TRAILR2 protein expression.
