title: Caractérisations moléculaires de rVSV-ZEBOV, vaccin prototype contre le virus Ebola
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  During EBOV infection GP1,2
  
  Plasma
  
  
  
  membrane
  
  GP
  
  1 GP2 Fur
  
  in 
  
  Glyc
  
  an
  
  cap Mu
  
  cin
  
  Gol
  
  gi
  
  apparatus TM Pre-
  
  GP
  
  Glyc cin TM Pre-GPER Endoplasmic reticulum Glycan cap Signal peptide Mucin TM Fusion peptide Cytoplasmic tail mi?>5 $! #(5 +,.+0!%* %/ "%./0 /5*0$!/%/! /,.! 1./+. %* 0$! * +,( /)%.!0% 1(1) X.!NY 3$% $ $ / %)) 01.! N #(5 +/5( 0%+*/X/$+3* 5 (1! %. (!/Y0$ 0.!)+/0(5 00 $! 0+0$!)1 %*N(%'! +) %*X Y,.!/!*0%*0$!"101.!g /1 1*%0M *0$!+(#%J0$!#(5 +,.+0!%* %#$) **+/!N#(5 +/5( 0%+*/.!) 01.! %*0+ +),(!405,!N#(5 */X.!NY * %/ (! 2! 50$!!((1(.!*65)!1.%*%*0+g * hJ3$% $ //+ % 0!/%*0+ %)!.0$ 0.!(%*'! 5 %/"1(! +1* M 0 0$%/,+%*0J %0 $ / +),(!4 N#(5 */ X.! %. (!/YM * h 03+ N#(5 +/5( 0%+*/ /%0!/!4%/0 10 +*(5 +*! $ / +),(!4N#(5 */ 00 $! 0+%0M$!) 01.!gJh%/ 0.%)!.%,.+0!%*%*/!.0! 0$.+1#$%0/0. */)!). *!
  
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OBK:E @ER<HIKHM>BGL :ELH @H>L BG MA> L>GL> H? : F><A:GBLF MA:M L>@K>@:M>L #, PBMALHF>IHKMBHG?:M>=?HKOBK:EI:KMB<E>?HKF:MBHG:G=MA>HMA>K?HKLA>==BG@"B@ %GMABLK>@:K= HNKK>LNEMLH;M:BG>=A>K>=>FHGLMK:M>MA:M :ME>:LMBGGHG BG?><M>=:G=BG?><M>= <>EEL )EH<:EBL:MBHGBL<HGMKHEE>=:MMA>IE:LF:F>F;K:G>K>LNEMBG@BG<E>:KLI:MB:E L>@K>@:MBHG ;>MP>>G :K>:L PAB<A :K> >GKB<A>= BG ) IKHM>BGL H? :K>:L =>IE>M>= H? )"B@ /N<AHK@:GBL:MBHGL<HNE=MA>K>?HK>E>:=MHMA>IKHM><MBHGH?:IHHEH?#, IKHM>BGMA:MPHNE=;>NL>=?HKMA>IKH=N<MBHGH?GHKF:EOBK:EI:KMB<E>LMA:MPHNE=G>O>K;>BG =:G@>KH?;>BG@<E>:O>=;RMA><>EENE:KLA>==:L> %MBLMA>K>?HK> BGM>K>LMBG@MHIHLMNE:M>MA:M #, BL :;E> MH M:D> :=O:GM:@> H? ) <ENLM>KBG@ MH >GLNK> MA> IKH=N<MBHG H? AB@AER BG?><MBHNLI:KMB<E>L=NKBG@MA>BG?><MBHG gfj am sa lP Shedding Viral release ADAM17 Viral budding compartment GP sh
  
  edding
  
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  GP1,2-RES GP1,2-RES is transported to the plasma membrane where it is accumulating in lipid rafts but is not incorporated into viral particles GP1,2-RES GP1,2-RES is synthesized Pre-GPER 6 GP1,2 GP1,2Δ During r VSV-GP infection ERK1/2 and p38 pathways are unregulated during the infection Maturation and processing of Pre-GPER into pre-GP and GP1,2 GP1,2 ADAM17 GP1,2 is either incorporated into viral particles or shed by ADAM17 GP1,2 GP1,2 is protected from shedding due to a compartmentalization of and ADAM17 GP1,2 Golgi
  
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