None
media for, 480 discovery of, 7 early investigation of, 477 examination for, by animal inoculation, 175 by Ziehl-Neelson staining method, 176 in feces, 178 in milk, 691 isolation of, 480 leprosy bacillus and, differentiation between, by stains, 106 methods of staining, 104, 105, 106 in sections, 112 cclloidin. 112 paraffin, 112 morphology of, 477 pathogenicity of, 483 fre^iiiency in, 483 mode of infection in, 484 mortality in, 483 preparation of, for agglutination tent, 251 quantitative analysis of, 22 Tubercle bacillus, smegma bacillus and, differentiation between, by stains, 106 staining of, 478 differentiation of, from acid-fast group by Pappenheim s method of, 480 Ehrlich's anilin- water-gentian- vio- let solution in, 479 I Gabbet 8 decoloration and coun- I terstaining in, 479 Ziehl's carl)ol-fuchsin solution in, 479 toxins of, 485 endotoxins in, 485 tuberculins in, 485 bouillon filtr^ (Denys), 487 "new tuberculin-harillary emul- sion" (Koch). 487 "new tul>erculin" (Kix?h), 48<l original methiHl of making of, 486 present method of making of, 486 "oW tulwculin" (Koch), 4K5 " tuberculoplasmin " ( Huchner and Halm), 487 Tuberculin. See under Tulx?rclc InumUun, toxins of " Tuberculoplasmin " (Murhner and Hahn), 4S7 Tuberculosis, froipiency of, 4Ki immunization in, passive, 492 Maragliano's senmi in, 492 Marmorek's scrum in, 493 mode of infection in, 484 mortality of, 48:^ tuberculin in, diagnoHtic use of, 4K7 cutaneous reaction in, 4K9 in cattle. 4S9 ophthalmo riMirtion in, 488 HubcutaneoiiM injoction of, 487 dosage and n^artion in. 488 therafMMitic uws of, 491 original, 491 prcwnt, 491 dosage in, 492 preparations employed in, 492 744 INDEX OF SUBJECTS Ubine, bacteriologioal examination of, 176 Urobacillus liquefaciens, 453 Uschinsky's proteid-free medium, 126 Vaccine production, for immunization in smallpox, 647 animals used in, 647 calves used for, 647 cleanliness observed in stabling of, 647 material used for vaccination of, 648 vaccination in, 648 preparation of field in, 648 scarifications in, 648 vaccinia vesicles developed in, 648 obtaining of vaodne from, 649 by curettag6i 649 by ivory tips, 649 testing of vaccine in, for bacteria, 650 for efl5ciency, 649 Vaccine therapy of Wright, 286 dosage for, 288 opsonic curve in, 288 production of vaccines- in, 286 standardization of emulsion in, 287 enumeration of bacteria against red blood cells in, 287-8 sterilization of vaccine in, 288 Variola. See Smallpox Vegetative forms from bacterial spores, 17 "Vibrion septique." See Malignant edema, bacillus of Vincent's angina, 599 8pirocha?te of, 599 cultivation of, 601 fusiform variety of, 600 bacilli of other diseases resem- bling, 602. See also under Fu- siform bacilli other bacilli accompanying, 602 spirillum variety of, 601 symptoms of, 599 V^inceiit's spirilla, staining of, 108 Virulence, definition of, 18.3 variations in, and infectiousness, 183 Virulent bacteria, sublethal doses of, in immunization, 195 Vini8 fixe, in specific therapy of rabies, 639 Waosworth's method of staining cap- sules, 99 Wassermann test for diagnosis of syph- ilis, 262 antigen for, 262 determination of necessary quantity of, 264 obtaining of, from alcoholic ex- tracts of syphilitic organs, 263 from alcohohc solution of normal organs, 263 from salt-solution of ssrphilitic Uver, 263 of syphilitic spleen, 262 preparation of, by Noguchi meth- od, 264 complement in, 266 hemolytic serum in, 265 obtaining of, 265 potency of, 265 quantity of, 265 unit in, definition of, 265 determination of, 266 modifications of, 268 Bauer's, 268 Noguchi's, 270 preparation for, 262 serum to be tested for syphilitic anti- body in, 267 sheep corpuscles in, 267 technique of, 267 Water, bacteria in, 671 in ground waters, 673 in perennial springs, 674 in wells. 673 in rain and snow, 672 in surface waters, 672 influence of rain on, 672 light and temperature factors in purification of, 673 self-purification in, 672 pathogenic, 671 of cholera, 671 of diarrheal diseases, 671 of typhoid fever, 671 INDEX OF SUBJECTS 745 Water, bacteria in, qualitative analysb of, 676 isolation of cholera vibrio in, 678 Koch's method of, 678 isolation of colon bacillus in, 679 isolation of typhoid bacillus in, 676 Adam's and Chapin's method of, 676 Drigalski's method of, 677 Parietti's methoti of, 677 Vallet's method of, 677 quantitative estimations of, 674 collecting of specimens for, 674 colon bacilli in, 680 colon test in, 679 ■ counting of bacilli in, 680 counting in, 676 incubation of specimens in, 676 plating of specimens in, 675 value of, 676 in bacterial cell, 21 Welch, discovery of Bacillus a^rogenes capsulatus by, 469 Welch's method of staining capsules, 98 Welch's modification of Guamieri's medium, 129 Wertheim's medium for cultivation of gonococcus, 381-2 Winckel's disease in the newborn due to colon bacillus, 394 Wires used in transfering bacteria, 141 Wolflfhiigel counting plate, 162 Wool-sorter's disease. 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