None
The epithelium of the olfactory region is of the columnar pseudo- stratified type, with several strata of nuclei, and consequently closely simulates a stratified columnar epithelium. Here we dis- tinguish olfactory cells and sustentacular cells. The olfactory cells occupy a peculiar position among the cells of special sense in that they represent true ganglion cells (Schultze, Golgi, Ehrlich, Ramon y Cajal). Within the epithelial layer they appear as spindle-shaped cells, with a spheric nucleus provided with a large nucleolus lying in the thickest portion of each cell. The nuclei of the different cells lie at varying levels in the middle stratum THE ORGAN OF SMELL. 499 of the epithelial layer. Toward the nasal cavity, the cells terminate in blunt cones, upon each of which are several stiff hairs, the olfac- tory hairs. The basilar ends form true centripetal nerve-processes, neuraxes, which end in the peculiar telodendria constituting the glo- meruli of the olfactory bulb. (See p. 422.) The nuclei of the sustentacular cells are more oval and are situ- ated at nearly the same level. These cells present the appearances of long columnar cells, which toward the basement membrane ter- minate in one or several processes. Between the basilar ends of these cells we find a layer of elements the broad nucleated bodies of which rest on the basement membrane, while their upper extremities terminate in short superficial processes. The mucosa contains a large number of leucocytes as well as •tiftfiL: -LV ' • "r : • ••• "•— '-V-- ?-.*s:w: -•-.«*..•,-' ,.';'- ssSPili^^- &'v-:>— '^^-^^^f^BI^BHte^V " '^.»°.v^r** Fig. 377. — Portion of transverse section of the olfactory region of man ; X I5° • A zone of olfactory hairs ; <?/>, epithelium ; 2, zone of oval nuclei ; j, zone of round nuclei ; ,<,-•/, olfactory or Bowman's glands; «, branch of olfactory nerve; //, mucosa or tunica propria with blood-vessels (Sobotta, "Atlas and Epitome of Histology"). numerous branched tubular glands, the so-called olfactory glands or the glands of Bowman. In man these are albuminous (serous) glands, and their cells sometimes contain pigment. Jacobson's organ consists of blindly ending tubes, situated at the lower portion and at the outer side of the nasal septum. It is lined by an olfactory mucous membrane and receives a branch of the nasal nerve. This organ is rudimentary in man. The capillaries spread out immediately beneath the basement membrane of the epithelium. In the submucous connective tissue, we find a relatively well developed vascular plexus, rich in venous vessels ; this plexus is especially marked at the posterior portion of the inferior turbinate bone, forming here a tissue which resembles erectile tissue. 5OO THE ORGAN OF SMELL. A dense network of lymphatics ramifies throughout the mucous membrane, carrying the lymph to the pharynx and palate. These lymph-vessels may be injected through the subarachnoid space (Key and Retzius). The nerves (trigeminal) are widely distributed in the epithelium, ramifying through both the respiratory and olfactory regions. After repeated divisions these nerves lose their medullary sheaths, and end in telodendria which are usually provided with terminal nodules, although some are found which end in mere filaments. TECHNIC The nasal mucous membrane is fixed in situ with osmic acid or one of its mixtures, after which small pieces are removed. It should be mentioned that the nonmedullated fibers of the olfactory nerve assume a brownish color under this treatment, while the fibers of Remak do not (Ranvier, 89). In order to isolate the epithelial elements, pieces of the mucous membrane are treated with the y^ alcohol of Ranvier. But since the prolongations of the olfactory cells (neuraxes) shrivel and curl in this fluid, Ranvier recommends that, after the epithelial cells have been macerated in y$ alcohol for one or two hours, they be treated with i % osmic acid for a quarter of an hour. If shreds be now placed in water and teased, the cells, together with their prolongations, may be isolated without the curling of the latter. The chrome-silver method applied to the nasal mucous membrane of young animals and fetuses has been the means of establishing the important fact that the olfactory cells of the olfactory region are in reality peripherally situated ganglion cells. NDEX. ABBE'S apparatus, 19 Absorption of fat by intestine, 288 method of studying, 306 Accessory disc of Engelman, 139 lacrimal glands, 470 thread of spermatosome, 361 Acervulus, 423 Acetic acid, effect on connective tissue, 128 on red blood-corpuscles, 188 sublimate solution as fixing fluid, 25 Achromatic portion of nucleus, 63 spindle, 68 Acidophile granules, technic for, 227 Adenoid connective tissue, 196 Adipose tissue, 107 stain for, 130 Agminated lymph-nodules, 197 Air-cells, 314 Air-spaces, ultimate, 313 Akrosome, 377 Alcohol as fixing solution, 23 as macerating solution, 22 Alcoholic borax-carmin solution as stain, 4i in bulk, 46 Alkalies, effect on red blood-corpuscles, 189 Altmann's method of demonstrating granules in cells, 77 of mounting, 78 process, 55 Alum-carmin as stain, 42 in bulk, 46 Alveolar ducts, 314, 315 glands, 91 compound branched, 91 simple, 91 branched, 91 periosteum, 242 Alveoli, 88 lung, 314 of mammary gland, epithelium of, 401 Amacrine cells, 464 Amitosis, 64, 70 Amitotic cell-division, 70 Amphiaster, 68 Amphipyrenin, 63 Amphophile granules, technic for, 228 Ampullae of Thoma, 204 Anaphases, 65, 69 Anastomoses, 222 Anilin stains, 44 Animals, injection of, 54 Anisotropic transverse disc, 138 Annulospiral nerve-ending, 178 Annulus fibrosus, 477 atrioventricularis, 214 Anterior epithelium of crystalline lens 468 ground bundle, 411 hyaloid artery, 468 lymph-channels of eye, 469 superior vertical canal, 480 Anterolateral columns, ascending, 411 descending, 411 Antrum of ovary, 347 Anus, 281 Apathy's method for demonstration of fibrillar elements of nervous system, 442 Apochromatic lens, 19 Aponeuroses, 105 Aqueous borax carmin solutions as stain, 4i humor, 446 Arachnoid, 437 Arches of Corti, 490 Arcuate fibers of cornea, 450 Area cribrosa, 332 vasculosa, 186 Areas of Langerhans, 301 Arrectores pilorum, 393 Arteriae arciformes, 332 capsulares glomeruliferae, 334 Arterial circle of Zinn, 465 retia mirabilia, 333 Arteries, 216 coronary, 214 hyaloid, anterior, 468 posterior, 468 interlobular, of kidney, 332 medium-sized, 218 of choroid, 455 of retina, 466 precapillary, 218 Arteriolae rectae spuriae, 333 verae, 334 Artery, auditory, internal, 494 central, of retina, 465 hepatic, 293 nasal, inferior, of retina, 466 superior, of retina, 466 papillary, inferior, of retina, 466 superior, of retina, 466 renal, 332 501 502 INDEX. Artery, temporal, inferior, of retina, 466 superior, of retina, 466 Ascending anterolateral columns, 411 Association fibers of cerebral cortex, 420 Astrocytes, 435 Atresia of ovarian follicles, 353 Atria, 314 Attraction-sphere, 62 Auditory artery, internal, 494 cells, outer, 491 hairs, 490 nerve, 494 ossicles, 478 teeth, 488 Auerbach's plexus, 286 Auriculoventricular valves of heart, 213 Axial canals of small intestine, 285 cords, 157, 1 60 fibrils of, demonstration of, 181 sheath, 176 thread of spermatosome, 361 sheath of, 361 Axillary glands, 398 Axis-cylinder, 159 naked, 160 Axis-fibrils, 157 Axolemma, 157 BAILLARGER'S striation, 421 Balsam, Canada, as mounting medium, 52 Bardeen's table for drawing of portions of sections to be reconstructed, 57 Bars of intercellular cement, 86 Bartholin's ducts, 253 glands, 360 - Basement membrane, 81, 88 of small intestine, 278 Basic stains, 41 Basichromatin granules, 62 Basilar membrane, 488 Basket cells, 254 Baskets, fiber, of retina, 462 Basophile granules, 193 cells with, 209 technic for, 228 Bechtereff and Kaes' striation, 421 Benda's chromatoid accessory nucleus, 377 method for demonstration of medul- lary sheath, 442 selective neuroglia staining method, 445 Berkley's method of demonstrating nerves of liver, 308 Berlin blue as injection fluid, 55 Bertini, columns of, 324, 330 Bethe's method of fixing methylene-blue for nerve-fibers, 184 of staining neuro fibrils and Golgi- nets, 443 Bile capillaries, 290, 291, 292 demonstration of, 306 impregnation of, 307 effect on red blood-corpuscles, 188 Bile-ducts, 296 Bioblasts, 60 Biondi-Heidenhain triple stain, 46 Bipolar cells of cone-visual cells, 463 of rod-visual cells, 463 Bismarck brown as stain, 44 Bladder, 336 nerves of, 339 technic of, 343 Blastema, 64 Blastodermic layers, primary, 79 Blastomeres, 70, 79 Blood, 1 86 coagulation of, 195 Blood, cover-glass preparations, 227 current, behavior of blood-cells in, 196 demonstration of, through vessels, 231 elements of, method of examining, 227 films, Wright's method of staining, 229 formation of, 186 islands, 186 plasma, 187 platelets, 194 fixation of, 227 shadows, 188 sinus, 222 supply of bronchi, 316 of Fallopian tubes, 355 of heart, 214 of intestine, 283 of lymph -glands, 200 of salivary glands, 259 of spleen, 203 of thymus gland, 212 of thyroid gland, 320 of uterus, 357 technic of, 226 Blood-cells, behavior of, in blood current, 196 counting, 232 red, nucleated, containing hemo- globin, 208 staining of, 227 Blood-corpuscles, cover-glass prepara- tions, 226 red, 187. See also Erythrocytes. technic of, 226 white, 191. See also Leucocytes. Blood-counting apparatus, Thoma-Zeiss, 232 Blood-forming organs, 186 technic of, 226 Blood-placques, 194 Blood-vessels, 186, 216 fetal, of eye, 468 in striated muscular tissue, 143 nerve supply of, 223 of bone-marrow, 210 of central nervous system, 439 of eyelid, 473 of kidney, 332 of liver, distribution of, demonstration, 306 examination of, 343 INDEX. 503 Blood-vessels of lung, 316 of membranous labyrinth, 494 of mucosa of large intestine, 284 of pelvis of kidney, 338 of small intestine, 284 of nasal cavity, 499 of optic nerve, 465 of ovary, 354 of pancreas, 302 of prostate, 370 of retina, 465 of sclera, 449 of stomach, 284 of suprarenal glands, 341 of teeth, 242 of testis, 367 Blutlymphdriisen, 200 Body-cell, 71 Bohmer's hematoxylin as stain for bulk, 46 hematoxylon, 42 Bone, 112 breakers, 120 calcium carbonate in, alkaline purpurin as stain for, 132 canaliculi, 113 compact, of shaft, development of, 124 corpuscles, 113 Schmorl's method of staining, 133 Virchow's method of isolating, 134 decalcification of, 132 fluids used for, 132 v. Cartilage, capsules of, gold chlorid as stain for, 131 connective tissue in, picrocarmin as stain for, 131 corrosive sublimate as fixative for, 130 cuneiform, 310 elastic fibers in, picrocarmin as stain for, 131 fibre-elastic, no glycogen in, iodo-iodid of potassium stain to demonstrate, 131 ground-substance of, change in, in hyaline, 108 of larynx, 310 of Wrisberg, 310 osmic acid a fixative for, 130 ossification of, in Caustic potash as macerating solution, 22 Cell, 58 absence of membrane, 62 air-, 314 amacrine, 464 auditory, outer, 491 basket, 254 blood, behavior of, in blood current, 196 counting of, 232 red, nucleated, containing hemoglo- bin, 208 staining of, 227 body-, 71 bone-, 112, 115 t Brownian movement of, 61 centro-acinal, 300 chief, of acini of thyroid gland, 320 of hypophysis, 423 chromophilic, of hypophysis, 423 ciliated, 60 colloid, of acini of thyroid gland, 320 commissural, 408 cone-visual, 459 bipolar cell of, 463 connective-tissue, fat producing, 97 fixed, 103 cortical, small, of cerebellar cortex, 415 crystals of, 61 cuneate, 301 definition of, 58 Deiter's, 491 demilunar, 257 diagram of, 59 diffuse, of retina, 464 double staining of, 76 enamel, 243 endothelial, 80, 94 and mesothelial, method of studying relations, 95 demonstration of, 95 technic for, 233 epithelial, in small intestine, 275 isolated, examination of, 95 fat of, 6 1 fat-, scheme of, 107 fixing of chromic acid for, 75 corrosive sublimate for, 75 Cell, Flemming's solution for, 75 picric acid for, 75 flagellated, 60 follicular, 372 ganglion, 149 demonstration of, 182 of Dogiel, in spinal ganglia, 426 giant, 209 glandular, 61 glycogen of, 61 goblet, 87, 265 granular, of cerebellar cortex, 416 granules in, Altmann's method of demonstrating, 77, 78 hair-, of utriculus, 483 hepatic, cords of, 290 horizontal, of retina, 464 liver-, examination of, 306 glycogen in, demonstration of, 306 lutein, 353 marrow-, 208 mast-, 104 granules of, technic for, 228 mesameboid, 80 mesothelial, 80 and endothelial, method of studying relations, 95 migratory, 103, 104, 193 mitosis of, demonstration of, 75 mitral, 421 of olfactory bulb, 421 molecular movement of, 61 monostratified, of retina, 464 mother, 374 mucus-secreting, 87 muscle-, cardiac, demonstration of, 148 nonstriated, 134 of fibers of Purkinje, 147 nerve-, 149. See also Ganglion cell. neuro-epithelial, 92 neurogliar, 434 of Bottcher, 492 of Cladius, 492 of column of Clark, 408 of Golgi, 408, 418, 419 of Hensen, 491, 492 of Langerhans, 300 of Leydig, 470 of Martinotti, 418, 419 of pancreas, inner and outer zones, methods of differentiating, 308 of Purkinje, 153 of cerebellar cortex, 415 of reticular connective tissue, 100 of Sertoli, 364 olfactory, 498 parareticular, 464 pigment, 61, 77, 104 pillar, 490 heads of, 490 inner, 490 outer, 490 plasma, 104 plurifunicular, 408 polarity of, 81 INDEX. 505 Cell, polygonal, of cerebral cortex, 417 polymorphous, of cerebral cortex, 418 polynuclear, 70 polystratified, of retina, 464 pyramidal, large, of cerebral cortex, 4i7 of cerebral cortex, 153 small, of cerebral cortex, 417 rod-visual, 458 bipolar cell of, 463 seminal, primitive, 372 sense, 81 sexual, fertilization of, 71 male, development of, 72 matured, 71 somatic, 71 spider, 435 spindle-shaped, of cerebral cortex, 417 staining of, 76 stellate, large, of cerebellar cortex, 416 of cerebellar cortex, 415 of cerebral cortex, 417 of liver, 295 sustentacular, 92, 250, 372, 483 tendon, from tail of rat, 107 visual, 458 wandering, 60, 103, 104 with basophilic granules, 209 with eosinophile granules, 209 Cell-bodies of neurones, 149 Cell-body, 59 Cell-division, 64 amitotic, 70 direct, 64, 70 indirect, 64 karyokinetic, heterotypic, 374 homeotypic, 374 mitotic, of fertilized whitefish eggs, 66, 67 ten stages of, 65 Cell-masses, intertubular, of pancreas, 301 Cell-microsomes, 59 Celloidin imbedding, 30 diagram for, 32 infiltration, 30 diagram for, 32 sections, cutting of, with sliding micro- tome, 36 dextrin method of fixing, 40 Celloidin-paraffin imbedding, 32 infiltration, 32 Cell-plate, 70 Cell-spaces of areolar connective tissue, 102 Cellular elements of areolar connective tissue, 103 Cement lines, 146 Cementum, 241, 246 Centers of ossification, 116 Central artery of retina, 465 gray nuclei of cerebellar cortex, 416 nervous system, 406 blood-vessels of, 439 fibrillar elements of, Apathy's method of demonstrating, 442 Central nervous system, lymph-vessels of, 440 membranes of, 436 technic of, 440 spindle, 68 vein of retina, 465 Centripetal fibers of cerebral cortex, 420 Centro-acinal cells, 300 Centrosomes, 62, 427 Centrospheres, 62, 427 Cerebellar columns, direct, 411. Chromosomes, 67 daughter, 68 Chrzonszczewsky's physiologic auto-in- jection, 306 Chyle-vessels, 285 Cilia, 81, 470 movement of, method of observing, 95 Ciliary body, 446, 452, 453 nerve supply of, 456 glands, 398, 454 of Moll, 470 muscle, 454 meridional division, 454 middle division, 454 third or inner division, 454 processes, 453 Ciliated cells, 60 epithelium, islands of, in cervical canal, 356 Circulation of hypophysis, 424 Circulatory system, 212 technic of, 235 Circulus arteriosus iridis major, 455 minor, 456 Circumanal glands, 398 of Gay, 282 Circumferential lamellae, inner, 113 outer, 113 Circumvallate papillae, 249 Clark's column, 408 cells of, 408 Claudius, cells of, 492 Clearing fluids, 52 Climbing fibers of cerebellar cortex, 416 Clitoris, 360 Cloquet's canal, 468 Club hairs, 393 Coagulation of blood, 195 Coal-tar stains, 44 Cochlea, 484 bony, 484 perilymph of, 496 spiral ganglion of, 494 technic for, 297 Cochlear duct, 484, 485 Cohnheim's fields, 140 method of impregnation, 48 Coil-glands, 396 Collective lens, 19 Colloid cells of acini of thyroid gland, 320 Colostrum, 402 corpuscles, 402 Columns, anterolateral, ascending, 411 descending, 411 cerebellar, direct, 411 lateral, 408 mixed, 411 of Bertini, 324, 330 of Burdach, 411 of Clark, 408 cells of, 408 of Goll, 411 of Gower, 411 of Sertoli, 364 pyramidal, crossed, 411 ventrolateral, 408 Columns, ventromesial, 408 Columns rectales Morgagni, 282 Commissural cells, 408 fibers of cerebral cortex, 420 Commissures of spinal cord, 412 Compound microscope, 17 Concentric lamellae, 113 Concretions of prostate, 370 Condensers, 19 Cone-fibers of retina, 459 Cone-visual cells, 459 bipolar cells of, 463 Coni vasculosi Halleri, 364 Conjunctiva, 469 scleral, 448 Conjunctival portion of eyelids, 470 Connective tissue, 96. action of acetic acid on, 128 of hydrochloric acid on, 128 of potassium hydrate on, 128 adenoid, 196 areolar, 101 cell-spaces of, 102 cellular elements of, 103 ground-substance of, 102 matrix of, 102 development of, schematic diagram of, 98 effect of pepsin on, 128 of trypsin digestion on, 127 fibrous, 101 white, 99 in cartilage, picrocarmin as stain for, J31 magenta red as stain for, 128 mucous, 100 of liver, 294 orcein as stain for, 128 Ranvier's method for examination of, 126 reticular, 100 cells of, TOO slide digestion of, 129 technic of, 126 Connective-tissue cells, fat producing, 97 fixed, 103 corpuscles, 103 fibrillae and reticulum, differential stain for, 128 framework of organs and tissues, digestion method for demonstrating, 129 Contraction-ring, 158 Conus medullaris, 406 Convoluted tubules of testes, 363 Cord, spinal, 406 See also Spinal cord. Cords, axial, 157, 160 fibrils of, demonstration of, 181 hepatic, 290 medullary, 199 of hepatic cells, 290 pulp, 204 Corium, 379, 382 Cornea, 446, 449 anterior elastic membrane of, 449 arcuate fibers of, 450 INDEX. 507 Cornea, epithelium of, 449 ground plexus of, 451 nerves of, 451 technic, 474 perforating fibers of, 450 posterior elastic membrane of, 450 subepithelial plexus of, 45 1 substantia propria of, 449 technic, 474 superficial plexus of, 451 Corneal corpuscles, 450 epithelium, technic of, 474 spaces, 450 technic of, 474 Corona radiata, 347 Coronary arteries, 214 Corpora amylacea of prostate, 370 lutea spuria, 353 Corpus albicans, 353 Highmori, 363 luteum, 353 verum, 353 Corpuscles, blood-, red, 187. See also Erythrocytes. blood-, white, 191. See also Leu- cocytes. bone, 113 SchmorPs method of staining, 133 Virchow's method of isolating, 134 colostrum, 402 connective-tissue, 103 corneal, 450 genital, 171 Golgi-Mazzoni, 388 Grandry's technic of, 405 Hassal's, 212 Herbst's, 174, 389 technic of, 405 Malpighian, 202, 203, 323, 324 Meissner's, 170 technic of, 405 Pacinian, 388 technic of, 405 tactile, 387 Vater-Pacinian, 173 distribution of, 174 Corrosive sublimate as fixative for car- tilage, 130 as fixing solution, 24 for fixing cells, 75 Cortex, cerebellar, 413. See also Cere- Cellar cortex. cerebral, 416. See also Cerebral cortex. of ovary, 344 Cortical cells, small, of cerebellar cortex, 4i5 layer of hair, 389 nodules, 198 substance of kidney, 323, 324 Corti's arches, 490 membrane, 489, 493 organ, 481, 489 spiral organ, 489 Cover-slips, 20 fixing of large number of paraffin sections to, 39 Cowper's glands, 370 Cox's method of impregnation, 51 Crescents of Gianuzzi, 256 Crista basilaris, 488 Cristae, 481 Crossed pyramidal columns, 411 Crosses, Ranvier's, demonstration of, 180 Crypts, Lieberkiihn's, 276 of stomach, 266 Crystalline lens, 467 anterior epithelium of, 468 Crystals, hematoidin, 231 hemin, method of obtaining, 230 hemoglobin, method of obtaining, 230 of cell, 61 Teichmann's, 188 method of obtaining, 230 Cuneate cells, 301 Cuneiform cartilages, 310 Cup, optic, 447 Cupola, 484 Cupula, 484 Currents of diffusion, 29 Cutaneous layer of tympanic membrane, 476 epidermis of, 476 Cuticle, 379 of hair, 389 inner, 389 Cuticula, 62, 81, 274 dentis, 238 Cuticular portion of eyelids, 470 ridge, 477 structures, 81 Cutis, 379. See also Skin. Cylindric end-bulb of Krause, 172 Czermak's interglobular spaces, 241 Czocor's cochineal solution, 42 DAMAR as mounting medium, 52 Daughter chromosomes, 68 nuclei, 64 stars, 374 Decalcification, 132 v. Ebner's method, 133 Decalcifying fluids, 132 aqueous solution of nitric acid, 133 hydrochloric acid, 132 Deiter's cells, 491 Delafield's hematoxylin, 43 Demilunar cells, 257 Demilunes of Heidenhain, 256 Dendrites, 149, 150 function of, 154 Dendritic fibrous structures of Gruber, 478 Dental sac, 244 Dentin, 239 development of, 244 fibrils of, demonstration of, 303 Dentinal fibers, 240 papillae, 243 tubules, 240 Dermis, 379, 382 508 INDEX. Descemet's membrane, 450 endothelium of, 451 technic of, 474 Descending anterolateral columns, 411 limb of Henle's loop, 327 Deutoplastic granules, 349 Dextrin method of fixing celloidin sec- tions, 40 paraffin sections, 40 Diapedesis, 193 Diaphragm, 17 iris, 1 8 Diaster, 69, 374 Diffuse cells of retina, 464 spongiblasts, 464 Diffusion, currents of, 29 Digestion method for demonstrating connective-tissue framework of organs and tissues, 129 slide, for connective tissue, 129 Digestive organs, 235 technic of, 303 tract, glands of, technic for, 304 Dilator muscle of pupil, 455 Direct cerebellar columns, 411 pyramidal tract, 411 Discus proligerus, 347 Dispirem, 69 Distilled water for fixing paraffin sections to slide, 39 Dorsal utriculus, 496 Double knife, 21 staining, 44 of cells, 76 Doyere's elevation, 162 Duct, alveolar, 314, 315 Bartholin's, 253 bile-, 296 cochlear, 484, 485 ejaculatory, 368 excretory, 367 Gartner's, 360 intralobular, of pancreas, 300 nasal, 474 pancreatic, 298 Steno's, 253 utriculosaccular, 481 Wharton's, 253 Wirsungian, 298 Wolffian, 360 Ductus endolymphaticus, 476 Dura mater, nerves of, 437 spinal, 436 EAR, 476 external, 476 technic for, 497 internal, 480 middle, 478 technic for, 497 technic for, 497 vestibule of, 480 Ectoderm, 79 tissues derived from, 79 Egg tubes, primary, of Pfluger, 345 Ehrlich-Biondi-Heidenhain three-color mixture, 229 Ehrlich's granulations, 227 hematoxylin, 43 for nuclei and granules, 228 leucocytic granules, 192 methylene-blue stain for nervous tissues, 182 neutrophile mixture, 229 Ejaculatory ducts, 368 Elastic elements, technic for, 235 fibers, 100 in cartilage, picrocarmin as stain for, 131 respiratory, demonstration of, 322 membrane, anterior, of cornea, 449 posterior, of cornea, 450 tissue, effect of trypsin digestion on, 127 method of obtaining, 127 Enamel, 238 cells, 243 germs, 243 prisms, 238 technic of, 303 Encoche d'ossification, 121 End-brush, 162, 167, 237 End-bulbs of Krause, 170, 388. Fibers, lens, 468 mantle, 69 mossy, of cerebellar cortex, 416 motor, 162 Miiller's, 454, 462 muscle-, intrafusal, 17^ nonstriated, demonstration of, 148 striated, technic of, 147 striped, 136 voluntary, development of, 144 nerve-, 157 ending in muscular tissue, 162 medullated, demonstration of. Foveolae of stomach, 266 Fragmentation, direct, of nucleus, 71 Freezing apparatus for sliding microtome, 36 Friedlander's glycerin-hematoxylin, 43 Front lens, 19 Fuchsin-resorcin elastic fibers stain, 128 Fundamental lamellae, 113 Fundus glands of stomach, 268 of fovea centralis, 460 Fungiform papillae, 248 Funiculi, of nerve-trunk, 160 compound, 162 Funnels, pial, 439 Future periosteum, 116 GANGLIA, 424 spinal, 424 sympathetic, 427 Ganglion cell, 149 demonstration of, 182 layer of retina, 459, 464 of Dogiel, in spinal ganglia, 426 spiral, of cochlea, 494 Gartner's duct, 360 Gastric mucous membrane, 266 Gastrulation, 79 Gay's circumanal glands, 282, 398 Gelatin-Berlin blue as injection fluid, 54 Gelatin-carmin as injection fluid, 54 Gelatinous bone-marrow, 210 substance of Rolando, 408 Genital corpuscles, 171 organs, female, 344 male, 361 Genito-urinary organs, 323 technic of, 342 Gerlach's method of impregnation, 48 Germ center, 197 of Flemming, 194 of lymph-follicles, technic for, 234 enamel, 243 hair, 389 layers, 58 primary, 79 Germinal epithelium, examination of, 378 of ovary, 345 vesicle, 71 Giant cells, 209 Gianuzzi, crescents of, 256 Giraldbs, organ of, 367 Gittcrfasern, 301 Glands, alveolar, 91. See also Alveolar glands. axillary, 398 capsule of, 92 carotid, 225 ceruminous, 389, 476 ciliary, 398, 454 Moll's, 470 circumanal, 282, 398 coil-, 396 extra-epithelial, 88 follicular, 91 hemal, 200 Glands, hemolymph, 200 structure of, 201 injection of, 55 intra-epithelial, 88 lacrimal, 473 accessory, 470 nerve supply of, 473 lenticular, 271 lymph-, 196, 197 blood supply of, 200 capsule of, 198 hilum of, 197 lymph-sinuses of, 199 marrow, 201, 202 technic for, 233 trabeculae of, 198 with blood-sinuses, 200 mammary, 400. See also Mammary gland. Meibomian, 472 mixed, 258 mucous, 255 multicellular, 88 classification, 91 of Bartholin, 360 of Bowman, 499 of Brunner, 265, 277 of Cowper, 370 of digestive tract, technic for, 304 of Gay, 282, 398 of Lieberkuhn's, 88, 276 of Moll, 398, 470 of Montgomery, 402 of mouth, small, 259 of oral cavity, 253 of skin, 396 of stomach, 267 cardiac, 267 fundus, 268 of Tyson, 372 parathyroid, 321 parotid, 255 peptic, 268 pineal, 422 prostate, 368. See also Prostate. pyloric, 269 salivary, 253, 255 blood supply of, 259 nerve supply of, 260 sebaceous, 398 serous, 255 splenolymph, 201 structure and classification, 88 sublingual, 255 submaxillary, 258 sudoriparous, 396. See also Sweat- glands. suprarenal, 339. See also Suprarenal glands. sweat-, 396. See also Sweat-glands. tarsal, 472 thymus, 210 blood supply of, 212 thyroid, 319. See also Thyroid gland. tubular, 89. See also Tubular glands. tubulo-alveolar, 90 512 INDEX. Glands, unicellular, 87 Glandula carotica, 225 Glandulas buccales, 236 duodenales, 265 labiales, 236 Glandular cells, 61 epithelium, 87 Glassy layer of choroid, 452, 453 membrane of hair, 391 Glisson's capsule, 289 Glomerular layer of olfactory bulb, 421 Glomeruli arteriosi cochleae, 495 Glomerulus, 323 Glomus caroticum, 225 Glycerin, Farrant's gum, mounting in, 53 mounting in, 53 Glycerin-albumen for fixing paraffin sec- tions to slide, 38 Glycogen in cartilage, iodo-iodid of potassium stain to demonstrate, 131 in liver-cells, demonstration of, 306 of cells, 6 1 Goblet cells, 87, 265 Gold chlorid as stain for capsules of car- tilage, 131 method of impregnation, 48 Golgi-Mazzoni corpuscle, 388 Golgi-nets, Bethe's method of staining, 443 Golgi's cells,- 408, 418, 419 chromsilver or chromsublimate method of impregnation, 49 gold chlorid method of impregnation, 48 methods of impregnation, 49, 50 mixed method of impregnation, 50 potassium bichromate and bichlorid of mercury method of impregna- tion, 50 method of impregnation, 49 preparations, Huber's method of per- manently mounting under cover- glass, 51 rapid method of impregnation, 50 slow method of impregnation, 50 Coil's column, 411 Gower's column, 411 Graafian follicle, 347 bursting of, 352 Grandry's corpuscles, technic of, 405 Granular cells of cerebellar cortex, 416 layer of cerebellar cortex, 416 of olfactory bulb, 421 Tomes', 246 sole plate, 163 Granulations of leukocytes, 227 Granules, acidophile, technic for, 227 amphophile, technic for, 228 basichromatin, 63 basophile, 193 cells with, 209 technic of, 228 chromatophile, 149 deutoplastic, 349 eosinophile, 193 cells with, 209 technic for, 227 Granules, in cells, Altmann's method of demonstrating, 77, 78 indulinophile, technic for, 228 interstitial, Kolliker's, 141 leukocytic, Ehrlich's, 192 neutrophile, 193 technic of, 228 oxychromatin, 63 Schron's, 344 tigroid, 149 zymogen, in pancreas, demonstration of, 308 Gray nuclei, central, of cerebellar cortex, 416 substance of spinal cord, 406, 409 Grenacher's alum-carmin, 42 as stain for bulk, 46 Ground bundle, anterior, 411 plexus of cornea, 451 Ground-substance interfascicular, 105 of areolar connective tissue, 102 of cartilage, changes, in, in Gruber's dendritic fibrous structure, 478 Gscheidtlen's method of obtaining hemo- globin crystals, 230 Gum glycerin, Farrant's mounting in, 53 HAIR, 389 auditory, 490 bulb, 389, 393 club, 393 cortical layer of, 389 cuticle of, 389 inner, 390 follicle, 389 nerve-fibers of, 393 germ, 389 glassy membrane of, 391 growth of, 392 medullary substance of, 390 olfactory, 499 papilla, 389 root, 389 root-sheaths of, 389 inner, 390 outer, 390 shaft, 389 shedding of, 393 technic for, 404 Hair-cells of utriculus, 483 Hamulus, 484 Hassal's corpuscles, 212 Haversian canals, 112 spaces, 120 Hayem's solution, 226 for diluting blood, 232 Hearing, organ of, 476. Heart, 186, 213 auriculoventricular valves of, 213 blood supply of, 214 elastic tissue of, 214 muscle, 145 motor nerve-supply of, 166 muscle-cells, demonstration of, 148 muscle-tissue, development of, 146 INDEX. 513 Heart, nerve supply of, 215 Heart-muscle fibers, MacCallum's nitric acid mixture for isolating, 23 Heidenhain's demilunes, 256 iron hematoxylin, 43 as stain for bulk, 46 median membrane, 137 Helicotrema, 485 Heliotropism, 61 Heller's plexus, 283 Hemal glands, 200 Hemalum, acid, as stain, 43 as stain, 43 in bulk, 46 Hematin, 187 Hematoblasts, 194 Hematoidin crystals, 231 Hematoxylin as stain, 42. See also Stains. Hematoxylin-eosin as stain, 45 Hematoxylin-safranin as stain, 46 Hemin, 188 crystals, method of obtaining, 230 Hemocytometer, Thoma-Zeiss, 232 Hemoglobin, 187 crystals, method of obtaining, 230 demonstration of, 230 nucleated red blood -cells containing, 208 Hemokonia, 195 Hemolymph glands, 200 structure of, 201 Henle's fiber layer, 461 layer, 390 loop, 323 descending limb of, 327 sheath, 162 Hensen's cells, 491, 492 median disc, 137 Hepatic artery, 293 cells, cords of, 290 cords, 290 Herbst's corpuscles, 174, 389 technic of, 405 Hermann's solution, 24 Heterotypic mitosis, 70 Hilum of lymph-glands, 197 Histology, general, 58 special, 186 Homeotypic mitosis, 70 Honing microtome knife, 37 Horizontal cells of retina, 464 semicircular canal, 480 Horns of spinal cord, 408 Howship's lacunae, 120 Hoyer's yellow gelatin mass, 54 Huber's method of permanently mount- ing Golgi's preparations under cover- glass, 51 Humor, aqueous, 446 Huschke's auditory teeth, 488 Huxley's layer, 390 Hyaline cartilage, 108 Hyaloid arteries, anterior, 468 posterior, 468 canal, 468 33 Hyaloid membrane of vitreous body, 467 Hydatids of Morgagni, 360 Hydrochloric acid, action of, on con- nective tissue, 128 as decalcifying fluid, 132 as macerating solution, 23 Hydrotropism, 61 Hymen, 359 Hypolamellar plexus, 261 Hypophysis, 423 chief cells of, 423 chromophilic cells of, 423 circulation of, 424 IMBEDDING, 27 celloidin, 30 diagram for, 32 celloidin-paraffin, 32 paraffin, 27 diagram for, 30 Immersion lens, 19 Impregnation, Cohnheim's method, 48 Cox's method 51 Gerlach's method, 48 gold chlorid method, 48 Golgi's chromsilver or chromsubli- mate method, 49 gold chlorid method, 48 methods, 49 mixed method, 50 potassium bichromate and bichlorid of mercury method, 50 potassium bichromate method, 49 rapid method, 50 slow method, 50 Kopsch's method, 52 Kuhne's method, 48 Lowit's method, 48 methods of, 47 of bile capillaries, 307 Ranvier's method, 48 silver nitrate method, 47 Indifferent fluids, 21, 22 Kronecker's, 22 physiologic saline solutions, 22 Ranvier's solution of iodin and po- tassium iodid, 22 Ripart and Petit's, 22 Schultze's iodized serum, 22 Indulinophile granules, technic for, 228 Inferior nasal artery of retina, 466 vein of retina, 466 papillary artery of retina, 466 vein of retina, 466 vertical semicircular canal, 480 I Infiltration, 27 celloidin, 30 diagram for, 32 celloidin-parafnn, 32 paraffin, 27 diagram for, 30 Injection fluids, 53 Altman's, 55 Berlin blue, 53 carmin mass, cold, 54 INDEX. Injection fluids, gelatin-Berlin blue, 54 gelatin-carmin, 54 silver nitrate, 55 yellow gelatin mass, 54 Injection method for demonstration of bile capillaries, 306 of distribution of hepatic blood- vessels, 306 methods of, 53 of animals, 54 of glands, 55 of lymph-channels, 55 of lymph-spaces, 55 of lymph-vessels, 55 of organs, 55 Inner molecular layer of retina, 464 nuclear layer of retina, 459, 462 scleral sulcus, 449 Intercellular bridges, 81, 380 demonstration of, 96 spaces, 81 substance, 79 Interfascicular ground-substance, 105 Interglobular spaces of Czermak, 241 Interlobular arteries of kidney, 332 veins of kidney, 334 of liver, 293 Intermediate tubule of pancreas, 300 Internal auditory artery, 494 ear, 480 limiting membrane of retina, 462 Interpapillary epithelial processes, 85 Interstitial granules of Kolliker, 141 Intertubular cell-masses of pancreas, 301 Intestine, 264 absorption of fat by, 288 blood supply of, 283 large, 281 blood-vessels of mucosa of, 284 lymph-vessels of, 284 lymph supply of, 283 mucous membrane of, general struc- ture, 264 nerves of, demonstration of, 306 muscularis mucosse of, 265 nerve supply of, 283 secretion of, 288 small, 274 axial canals of, 285 basement membrane of, 278 epthelial cells in, 275 lymphatics of, 285 lymph-nodules of, 279 mucous membrane of, 274, 277 blood-vessels of, 284 epithelium of, 274 leucocytes in, 275 lymph -nodules of, 279 villi of, 274 muscularis mucosje of, 279 villi of, lacteals of, 285 with villi, fixation of, 305 stratum circulare of, 266 fibrosum of, 265 longitudinale of, 266 submucosa of, 265 Intestine, tunica mucosa of, 265 Intima, endothelium of, technic for, 235 Intracapsular plexuses, 429 Intracartilaginous bone, 116 Intra-epithelial glands, 88 Intrafusal muscle-fibers, 175 Intralobular duct of pancreas, 300 Intramembranous bones, 116, 122 bone-development, 122 lodo-iodid of potassium stain to demon- strate glycogen in cartilage, 131 Iris, 446, 452, 455 anterior endothelium of, 455 diaphragm, 18 nerve supply of, 456 posterior epithelium of, 455 stroma of, 455 Islands of ciliated epithelium in cervical canal, 356 Isotropic intermediary disc, 138 JACOBSON'S organ, 499 Japanese method of fixing paraffin sec- tions to slide, 39 Jelly, Wharton's, 100 KAES and Bechtereff's striation, 421 Karyokinesis, 64 Karyokinetic cell-division, heterotypic, 374 homeotypic, 374 Karyolysis, 74 Karyosomes, 63 Keratohyalin, 380 technic for, 403 Kidney, 323 arched collecting portion of tubules, 323 329 blood-vessels of, 332 cortical substance of, 323, 324 distal convoluted portion of tubules, 323. 515 Kolliker's neuropodia, 151 Kollmann's cold carmin mass, 54 Kolossow's method of demonstrating in- tercellular bridges, 96 Kopsch's method of impregnation, 52 Krause, end-bulbs of, 170, 388 cylindric, 172 membrane of, 137 Kronecker's fluid, 22 Kuhne's method of impregnation, 48 Kupffer's stellate cells, 295 Kytoblastema, 64 LABIUM tympanicum, 487 vestibulare, 487 Laboratory microtome, 33 Labyrinth, bony, 480 development of, 496 membranous, 480, 481 blood-vessels of, 494 technic for, 497 osseous, 480 technic for, 497 Lacrimal apparatus, 473 glands, 473 accessory, 470 nerve supply of, 473 sac, 474 Lacteals of villi of small intestine, 285 Lacunae, Howship's, 120 of bone, 112, 113 Lamellae, 105 bone, 113 compostion of, 114 method of examining, 131 circumferential, inner, 113 outer, 113 concentric, 113 fundamental, 113 periosteal, 113 Lamina basilaris propria, 489 choriocapillaris, 452, 453 cribrosa, 448, 465 elastica interna, 218 fusca, 448 propria of oral cavity, 236 of tympanic membrane, 477 reticularis, 489, 493 spiralis membranacea, 485 ossea, 484, 486 suprachoroidea, 448, 452 vasculosa Halleri of choroid, 452 Langerhans, areas of, 301 cells of, 300 Large intestine, 281. See also Intestine, large. Larynx, 309 cartilages of, 310 demonstration of, 322 mucous membrane of, 309 nerves of, 311 vascular supply of, 310 Lateral column, 408 mixed, 411 Ledges, terminal, 86 Lens, 446 apochromati c, 19 capsule, 468 collective, 19 crystalline, 467 anterior epithelium of, 468 fibers, 468 ' front, 19 immersion, 19 ocular, 19 suspensory ligament of, 467 technic of 475 Lenticular, glands, 271 Leucocyte-nucleus, polymorphism of, 193 Leucocytes, 191 granulations of, 227 in epithelium of mucous membrane of small intestine, 275 method of counting, 232, 233 mononuclear, 192 motility of, 193 size of, 192 transitional, 192 with polymorphous nuclei, 192 Leucocytic granules, Ehrlich's, 192 Leydig's cells, 470 Lieberkiihn's glands, 88, 276 Ligament, suspensory, of lens, 467 Ligaments, 105 Ligamentum nuchse of ox, structure of, 1 06 pectinatum iridis, 454 spirale, 485, 488 Limbus spiralis, 486, 487 Lime-salts in bone, hematoxylin as stain for, 132 isolation of, 132 Limiting membrane, external, of retina, 459, 462 internal, of retina, 462 Lines of Retzius, 239 of Schrager, 239 Lingual mucous membrane, 247 papillae of, 247 papillae, 247 Linin, 63 Liquor folliculi, 347 Liver, 289 blood-vessels of, distribution of, dem- onstration, 306 examination of, 343 connective tissue of, 294 interlobular veins of, 293 lobules, 289 lymphatics of, 297 nerves of, 298 demonstration of, 308 reticular fibers of, demonstration of, 308 reticulum of, 294 stellate cells of, 295 technic of, 306 tissue, technic of, 307 trabeculae of, 290 Liver-cells, examination of, 306 glycogen in, demonstration of, 306 Loop, Henle's, 323 INDEX. Loop, Henle's descending limb of, 327 Lo wit's method of impregnation, 48 Lugol's solution to demonstrate glycogen in cartilage, 131 Lung alveoli, 314 blood-vessels of, 316 lobules of, 316 lymphatics of, 317 nerves of, 317 structure, units of, 316 tissue of, demonstration of, 322 Lunula, 395 Lutein, 353 cells, 353 Lymph, 186 canalicular system, 102 capillaries, 224 Lymphatic networks in endocardium, 215 system, 223 Lymph-channels, anterior, of eye, 469 injection of, 55 Lymph-follicles, germ centers of, technic for, 234 of mucosa of vermiform appendix, 281 of tongue, 251 of tonsils, 251 solitary, 197 technic for, 306 Lymph-glands, 196, 197 blood supply of, 200 capsule of, 198 hilum of, 197 lymph-sinuses of, 199 marrow, 201, 202 technic for, 233 trabeculae of, 198 with blood-sinuses, 200 Lymph-nodules, 196 agminated, 197 of mucosa of small intestine, 279 Lymphocytes, 191, 194 size of, 192 Lymphoid tissue, 196 Lymph-sinus, 199 Lymph-spaces, 224 injection of, 55 periaxial, 176 perichoroidal, 452 Lymph-supply of intestine, 283 Lymph-vessels, 186, 223 injection of, 55 of central nervous system, 440 of kidney, 334 of large intestine, 284 of liver, 297 of lung, 317 of mammary glands, 402 of mouth, 260 of ovary, 354 of skin, 386 of small intestine, 285 of testes, 367 of uterus, 357 MACCALLUM'S nitric acid mixture, 23 Macerating solutions, 22 alcohol, 22 caustic acid, 22 potash, 22 chromic acid, 22 hydrochloric acid, 23 MacCallum's, 23 nitric acid, 23 and chlorate of potassium, 23 sulphuric acid, 23 Maceration, methods of, 22 Macula acustica sacculi, 481 utriculi, 481 lutea, 460 region of, 460 Magenta red as stain for connective tissue, 128 Male genital organs, 361 pronucleus, 73 Mallory's differential stain for connec- tive-tissue fibrillae and reticulum, 128 selective neuroglia fiber-staining meth- ods, 445 Malpighian corpuscles, 202, 203, 323, 324 pyramid, 323 Mammary gland, 400 alveoli of, epithelium of 401 lymphatics of, 402 nerves of, 402 Mantle fibers, 69 Marginal thread of spermatosome, 361 zone, 8 1 Marrow, bone-, 207. See also Bone- marrow. fat-, 207 lymph-glands, 102, 202 spaces, primary, 118 secondary, 120 Marrow-cells, .208 Martinotti's cells, 418, 419 Mast-cells, 104 granules, technic of, 228 Matrix of areolar connective tissue, 102 of nail, 394 sulcus of, 394 Mature ovum, 351 Mayer's solutions for staining mucin, 305 Median disc of Hensen, 137 membrane of Heidenhain, 137 Mediastinum testis, 363 Medullary cords, 199 rays, 323 sheath, 157 technic, 440 Benda's, 442 Pal's, 442 Weigert's, 440, 441 substance of cerebellar cortex, 416 of cerebral cortex, 419 of hair, 390 of kidney, 323 of ovary^ 344 Meibomian gland, 472 Meissner's corpuscles, 170, 387 technic of, 405 INDEX. 517 Meissner's plexus, 287 Membrana capsulopupfllaris, 468 praeformativa, 244 prima, 81 propria, 88, 92 of pancreas, 301 of uriniferous tubules, 330 pupillaris, 468 tectoria Cortii, 489, 493 Membrane, basement, 81, 88 of small intestine, 278 basilar, 488 Bowman's, 449 cell, absence of, 62 Cord's, 489, 493 Descemet's, 450 endothelium of, 451 technic of, 474 elastic, anterior, of cornea, 449 posterior, of cornea, 450 external limiting, of retina, 459, 462 fenestrated, 107 glassy, of choroid, 452, 453 of hair, 391 Heidenhain's, 137 hyaloid, of vitreous body, 467 internal limiting, of retina, 462 Krause's, 137 median, of Heidenhain, 137 mucous. See also Mucous membrane. Nasmyth's, 238 nuclear, 63 of central nervous system, 436 oral mucous, fixation of, 303 otolithic, 483 peridental, 242 pigment, of eye, 446, 447, 457 Reissner's, 485, 489 rudder, of spermatosome, 361 tympanic, 476. See also Tympanic membrane. undulating, of spermatosome, 361 vestibular (Reissner's), 485, 489 vitreous, 452, 453 Meninges of central nervous system, 436 Menisci, tactile, 387 technic of, 405 Merkel's terminal disc, 139 Mesameboid cells, 80 Mesenchymatous tissue, 97 Mesenchyme, 80 Mesoderm, 58, 79 cells of, 80 Mesothelial cells, 80 and endothelial cells, method of studying relations, 95 Mesothelium, 80, 92 Metakinesis, 68 Metaphases, 65, 68 Methylene-blue stain, Ehrlich's, for nervous tissues, 182 for nerve-fibers, 184 Methyl-green as stain, 44 Metschnikoff's phagocytes, 60 Meyer's method of staining nerve- fibers, 184 Microcentrum, 191 Microscope and its accessories, 17 coarse adjustment of, 18 compound, 17 description of, 17 fine adjustment of, 18 parts of, 17 simple, 17 Microscopic preparation, 21 preparations of undecalcified bone, 131 technic, introduction to, 17 Microtome, 32 knife, honin? of, 37 sharpening of, 37 laboratory, 33 Minot automatic precision, 33, 34 rotary, 34, 35 precision, 33 rocking, 33 rotary, 35 sliding, 33 cutting celloidin sections with, 36 paraffin sections with, 35 freezing apparatus for, 36 Middle ear, 478 technic for, 497 Migratory cells, 103, 104, 193 Milk, 402 secretion, 401 Minot automatic precision microtome 33> 34 rotary microtome, 34, 35 Mitochondria, 60 Mitosis, 64 demonstration of, 75 heterotypic, 70 homeotypic, 70 Mitotic cell-division of fertilized white- fish eggs, 66, 67 ten stages of, 65 Mitral cells, 421 of olfactory bulb, 421 Mixed gland, 258 lateral column, 411 Modified sweat-glands, 398 Modiolus, 484 Molecular layer, inner, of retina, 464 of cerebellar cortex, 413 of cerebral cortex, 417 of olfactory bulb, 421 outer, of retina, 45, 462 movement of cells, 61 Moll's glands, 398, 470 Monaster, 68 Mononuclear leucocytes, 192 Monostratified cells of retina, 464 Montgomery's glands, 402 Morgagni, hydatids of, 360 Morula mass, 79 Mossy fibers of cerebellar cortex, 416 Mother cell, 374 nucleus, 64 skein, 67 Motor end-plate, 163 fibers, 162 nerve-endings, 162 5i8 INDEX. Motor nerve-endings, staining of, 182 neurones. 153 peripheral, 162 diagram of, 163 Mounting, 21, 52 Mouth, glands of, small, 259 lymphatics of, 260 Muchematein, 305 Mucicarmin, 305 Mucin, demonstration of, 304, 305 staining of, 305 Mucous connective tissue, IDC gland, 255 layer of tympanic membrane, 478 membrane, gastric, 266 intestinal, general structure of, 264 nerves of, demonstration of, 306 nasal, technic of, 500 of Eustachian tube, 479 of Fallopian tubes, 354 of larynx, 309 of oral cavity, 236 of pelvis of kidney, 337 blood-vessels of, 338 of small intestine, 274, 277 blood-vessels of, 284 epithelium of, 274 leucocytes in, 275 lymph-nodules of, 279 villi of, 274 of stomach, fixation of, 305 of tongue, 247 of uterus, 355 of vagina, 358 epithelium of, 358 of vermiform appendix, lymph - follicles in, 281 oral, fixation of, 303 Mucus-secreting cell, 87 Miiller's fibers, 454 of retina, 462 fluid, 26 Multicellular glands, 88 classification, 91 Muscle and tendon, relation of, method of studying, 148 ciliary, 454 dilator, of pupil, 455 heart, 145 fibers of, MacCallum's nitric acid mixture for isolating, 23 motor nerve-supply of, 166 nonstriated, motor nerve-supply of, 1 66 red, 141 .sphincter, of pupil, 455 striated, nerve-endings in, Sihler's method of demonstrating, 184 white, 141 Muscle-cells, cardiac, demonstration of, 148 nonstriated, 134 of fibers of Purkinje, 147 "Muscle-columns of Kolliker, 140 Muscle-fibers, intrafusal, 175 nonstriated, demonstration of, 148 Muscle-fibers, striated, technic of, 147 striped, 136 voluntary, development of, 144 Muscular coat of Fallopian tubes, 355 of uterus, 356 of vagina, 359 tissue, 134 destruction of, 144 development of, 144 heart, development of, 146 nerve-fibers ending in, 162 striated, blood-vessels in, 143 technic of, 147 Muscularis mucosae of intestine 265 of small intestine, 279 of stomach, 271 Musculus ciliaris Riolani, 471 orbicularis oculi, 471 palpebralis superior, 472 Myelin sheath, 157 Myelocytes, 208 Myeloplaxes, 209 Myoblasts, 144 Myocardium, 213 NAIL, 394 bed, 394 sulcus of, 394 body of, 394 lunula of, 395 matrix, 394 sulcus of, 394 root, 394 walls, 394 Nasal artery, inferior, of retina, 466 superior, of retina, 466 cavity, 498 blood-vessels of, 499 nerves of, 500 technic of, 500 vestibule of, 498 duct, 474 mucous membrane, technic of, 500 vein, inferior, of retina, 466 superior, of retina, 466 Nasmyth's membrane, 238 Nerve end-organs, neuromuscular, 174 neurotendinous, 1 78 Nerve-cells, 149. See also Ganglion cell. Nerve-endings, annulospiral, 178 flower-like, 178 in striated muscle, Sihler's method of demonstrating, 184 motor, 162 staining of, 182 peripheral, 162 Ruffini's, 387 sensory, 1 66 encapsulated, 169 free, 168, 169 staining of, 182 Nerve-fibers, 157 ending in muscle tissue, 162 layer of retina, 464 INDEX. Nucleus, leucocyte-, polymorphism of, 193 mother, 64 of spermatid, 377 resting, 63 segmentation, 71 sole, 163 telolemma, 163 Nuel's space, 492 OCULAR lens, 19 Odontoblasts, 240, 241, 244 Odontoclasts, 247 Olfactory bulb, 421 glomerular layer of, 421 granular layer of, 421 mitral cells of, 421 molecular layer of, 421 peripheral fibers of, 421 cells, 498 hairs, 499 region, 498 epithelium of, 498 nerve, fibers of, staining of, 182 Oocytes, 350 Optic cup, 447 nerve, 446, 464 blood-vessels of, 465 papilla, 460 region of, 460 stalks, 446 vesicles, primary, 446 secondary, 447 Ora serrata, 461 Oral cavity, 235 glands of, 253 mucous membrane of, 236 fixation of, 303 submucosa of, 236 Orbiculus ciliaris, 453 Orcein as stain for connective tissue, 128 Osmic acid as fixative for cartilage, 130 as fixing solution, 24 as stain for adipose tissue, 130 Osseous labyrinth, 480 Ossicles, auditory, 478 Ossification, centers of, 116 groove, 121 of cartilage, in ridge, 121 Osteoblasts, 118 Osteoclasts, 120 Otolithic membrane, 483 Otoliths, 483 Outer fiber layer of retina, 461 molecular layer of retina, 459, 462 Ovarian tissue, fixation of, 378 Ovary, 344 antrum of, 347 blood-vessels of, 354 cortex of, 344 germinal epithelium of, 345 lymphatics of, 354 medullary substance of, 344 nerves of, 354 Ovary, stratum granulosum of, 345 stroma of, 344 technic of, 378 Ovula Nabothi, 356 Ovum, 71, 344 changes in, during development, 350 mature, 351 primitive, 345, 350 ripe, 350 technic for, 378 vacuole of, 344 Oxychromatin granules, 63 PACCHIONIAN bodies, 438 Pacinian corpuscles, 388 technic of, 405 Pal's method for demonstration of medullary sheath, 442 Pancreas, 298 blood-vessels of, 302 cells of, inner and outer zones, methods of differentiating, 308 intermediate tubule of, 300 intertubular 'cell-masses of, 301 intralobular duct of, 300 membrana propria of, 301 nerves of, 302 technic of. 