None
In answer to many inquiries regarding the material used in this process, I may say that Messrs. Eimer & Amend, chemists, of New York, from whom my supply was originally obtained, state that the article furnished me was the Japan wax. Dr. J. W. Black- burn, of Washington, D. 0., kindly informs me that this is the product of Rlius succedanea. The material with which I have had the best results was of a very pale yellow or canary color. The darker specimens are unsuitable. KARYOKINESIS. The phenomena attending cell-division are best shown in the thin gill-plates or caudal fin of larvae of the salamandra. Very fair demonstrations may be made from rapidly-growing tumors, as carcinomata, if after removal they are sliced thin and immediately fixed. Flemming's Fixing Fluid* Ohromicacid, 0.25 per cental Osmic acid, 0.1 " > in water. Glacial acetic acid, 0.1 " Half an hour's immersion will usually suffice, after which thie tissue is rinsed quickly in water and transferred to absolute alco- hol, where it may remain until ready to cut. The parts of larvae, above mentioned, are of course sufficiently thin without sectioning. * "Microtomist's Vade-mecum," Lee. London, 1885. 202 PRACTICAL MICROSCOPY. For staining, haema. or picro-carmine answer well, although sanranin is preferred by Strassburger. A saturated solution of the dye in absolute alcohol is diluted with an equal volume of water and allowed to act on the tissue for twenty-four hours. Wash thoroughly with absolute alcohol, clear in oil of cloves, and mount in dammar. The highest powers are necessary for successful demonstration. FIXING AND STAINING THE CORPUSCULAR ELE- MENTS OF BLOOD. The following method, which has been elaborated by Prof. Gaule, of Zurich, will prove more satisfactory than processes which involve the drying of the blood. The essential steps are : 1. Transferrence to the slide. This must be accomplished very quickly to provide against changes in the corpuscular elements, which occur soon after removal from the vessels. If to be taken from the living animal — and the frog is best for beginners — the surface must be scrupulously clean, a small vessel punctured with the needle, and a minute drop of blood carried to the surface of a slide by means of a glass rod. The blood is spread in a thin layer^ after which, and before drying, the elements are to be fixed. 2. Fixing. — A portion, say fl. 5 ij., of a saturated aqueous solu- tion of bichloride of mercury having been prepared in a saucer, the blood slide is submerged in the liquid. Five minutes suffice for this work. 3. Washing is accomplished by immersing, the slide for a mo- ment in a saucer of distilled water, after which the action is com- pleted by placing in absolute alcohol for five minutes. Drain on bibulous paper for a moment. 4. Staining. — Moisten the blood with a little distilled watery drain, and afterward drop a few minims of haema. solution on the horizontally placed slide. Ordinary haema. will answer perfectly if, to about a drachm of the solution, two drops of alcohol are added. The staining is complete in five minutes. Wash in dis- tilled water, and again stain as before with a one-per-cent aqueous solution of nigrosin. Again wash with distilled water, and again stain as before with eosin one part, alcohol 50 parts, distilled water 150 parts, for one minute. 5. Mountin —A permanent specimen is completed by washing the film of blood with strong alcohol. A drop of oil of cloves gives FIXING BLOOD-CORPUSCLES. 208 translucency in a moment, after which it is drained oft', a drop of dammar added, and the cover applied. The nuclei of the red corpuscle of the frog take the blue hsema., while a variety of white corpuscles with a large round or spindle- shaped nucleus — the licematoblast of Hay em — has its protoplasm stained blue-black by the nigrosin. Ehrlich has given the name eosinopMlous cells to those white corpuscles with several nuclei whose granular protoplasm takes the eosin deeply. Other forms of colorless blood -corpuscles as amcebocytes and endotlielioid cells are differentiated by this mode of fixing and triple staining. The high- est powers of the microscope are required. INDEX Abbe* condenser, 4 Abdominal cavity, 162 salivary gland, 159 Aberration, chromatic, 2 spherical, 2 Absorption from intestine, 88 Acetate of copper, 200 Acid, acetic, 31 chromic, 22 hydrochloric, 22 nitric, 22 osmic, 49 osmic, solution, 200 picric, 26 Acini, compound, 155 simple, 155 Acinous glands, 155 Adenoid reticulum, 167 tissue, 61, 167 tissue in thymus body, 178 tissue, Klein on, 167 Adipose tissue in bronchi, 99 Adventitia of arteries, 66 Afferent glomerular arterioles, 125 Agents, staining, 25 Agminate glands, 91 Ailantus pith, 13 Air, atmospheric, 100 bubbles, 36 sacs, 100 vesicles, 100 Albuminate of silver, 164 Albuminous cement, 163 Alcohol " A," " B," and " C," 21 hardening, 20 Alum, 25 Alveoli, gland, 155 pulmonary, 100 Arnoebocytes, 203 Ampulliform dilatation, 97 Aniline colors, 198 Anterior commissure, 185 median fissure, 185 Appendages of skin, 71 Appendix, xiphoid, 58 Arachnoid, 191 Arcade, arterial, 124 Areas, physiological, of spinal cordr 185 Areolar tissue, 51 Arrectores pili, 74 Arrovyroot starch, 38 Arterial arcade, 124 Arteries, 66 adventitia of, 66 intima of, 66 large, 67 lymphatics of, 162 media of, 66 Arteriolse rectse, 126 Artery, bronchial, 95, 98 hepatic, 107 phrenic, 150 pulmonary, 99 renal, 124, 150 splenic, 173 typical, 66 Articular cartilage, 56 Artificial gastric fluid, 22 Ascending limb of Henle, 123 Asphaltum, 198 Atmosphere, 100 Attachment, freezing, 16 Auerbach's plexus, 86, 91 Author's microtome, 15 Axis cylinder, 180 Bacteria in urine, 37 Balsam, xylol, 197 Basement membrane, 99 membrane of corium, 71 Bayberry tallow, 22, 201 Beaker cells, 97 Bellini, tubule of, 124 Bergamot oil, 27 Bichromate of potash, 22 Bile capillaries, 108 ducts, origin of, 118 Bipolar cells, 50 nerve cells, 181 Birds, blood of, 48 Blackburn, Dr. J. W., 201 Black japan, 198 varnish, 198 206 INDEX. Bladder of frog, 62 urinary, 149 Blood corpuscle as test, 7 corpuscle, colorless, 49 corpuscle, red, 47 corpuscles, staining, 203 corpuscles, white, 49 fixing and staining, 202 of birds, 48 of camelidae, 48 of fishes, 48 of invertebrates, 48 of reptiles, 48 oxygenation of, 100 plates, 48 supply, hepatic, 106 supply of ovary, 149 supply of spleen, 173 vessels, 66 vessels of bronchi, 98 vessels of epineurium, 182 vessels of glands, 150 vessels of kidney, 124 vessels of omentum, 67 vessels of pulmonarv alveo- lus, 67 vessels of skin, 70 vessels of spinal cord, 189 vessels, pulmonary, 99 Bodies, Malpighian, of kidney, 122 Malpighian, of spleen, 173 Body, suprarenal, 120, 150 thymus, 177 Bone, 58 * corpuscles, 59 decalcifying, 22 mounting of, 61 Borax carmine staining fluid, 26 carmine staining process, 30 Bowman, capsule of, 122 Bowman's muscle discs, 65 Brain, 191 ganglia of, 191 gray matter of, 191 hardening of, 192, 200 lymph cavities of, 191 membranes of, 191 neuroglia of, 182 section cutting of, 192 sulci of, 191 white matter of, 191 Branched tubular glands, 154 Bronchial artery, 98 glands, 96, 154 tube, 94 vein, 98 Bronchi, 94 adipose tissue in, 99 basement membrane of, 99 blood-vessels of, 98 cartilage in, 98 glands of, 98 Bronchi, mucosa of, 98 muscular coat of, 98 nerves of, 98 staining of, 97 subdivision of, 94 terminal, 97, 101 Bronchus, layers of, 95 of pig, 44 Brood nests, 179 Brownian movement, 36 Brunner's glands, 89 Bubbles, air, 36 Buccal epithelium, 41 Cable, telegraphic, 181 Calyces of kidney, 120 Camelidse, 48 Canal, central, 186 Canaliculi, 58 Canal, portal, 108 Canals, dentinal, 78, 81 Haversian, 59 Cancer, epithelial, 179 Capillaries, 66 bile, 108 lymphatic, 161 of glands, 153 of ovary, 149 of supra-renal body, 150 stomata of, 67 tortuous, in lung, 103 Capsule of Bowman, 122 of Grlisson, 106 of kidney, 120 of lymph nodes, 167 of thymus body, 177 supra-renal, 150 Carcinomata, 201 Cardiac gland-tubes, 84 muscular fibre, 65 Care of objectives, 34 of the microscope, 34 Carmine and picric acid staining, 31 movement of particles of, 36 No. 40, 26 staining fluid, 26 Carpenter, Prof. Wesley M., 11 Cartilage, 56 articular, 56 corpuscles, 56 elastic, 57 fibro, 56 hyaline, 56 plates in bronchi, 98 reticular, 57 Casts, urinary, preserving, 199 Caudal fin, 201 Cavities, lymphatic, 161 Cavity, abdominal, 162 INDEX. 207 Cavity, cranial, 191 spinal, 191 thoracic, 162 Cell body, 39 cement, 163 distribution, 40 division, 201 primordial, 147 proliferation, 40, 201 structure, Ehrlich on, 203 typical, 146 wall, 39 Celloidin infiltration, 23 Cells, beaker, j~ bipolar, 50 bipolar nerve, 181 border, 85 central, 85 chief, 85 ciliated, 45 ciliated from oyster, 45 columnar, 41 covering, 51 Deiter's, 183 €ndothelial, 51 endothelioid, 203 «osinophilous, 203 epithelial, 51 flat, 41 ganglion, 181 glandular, 51 goblet, 97 hepatic, 109 in urine, 143 lining, 51 lymphoid, 61, 161 mucous, 97 multinucleated in thyrnus body, 179 mult i polar, 50 multipolar nerve, 181 nerve, 50, 181 of Purkinje, 194 outlining of, by silver ni- trate, 164 parietal, 85 polar, 47, 50 polyhedral, 49 quadripolar nerve, 181 spheroidal, 47 spider, 183 stellate, 47, 50 tailed, from pelvis of kidney, 140 teased, hepatic, 113 tripolar, 50 tripolar nerve, 181 typical, 39 unipolar, 50 unipolar nerve, 181 uterine, 136 vacuolated, of bladder, 142 Cells, vaginal, 136 variation in form, 40 Cement, cell, 163 zinc, 198 Central canal, 186 Cerebellar tracts, direct, 185 Cerebellum, 194 cortex of, 194 folia of, 194 Cerebral layers, 192 Cerebro-spinal system, 180 Cerebrum, practical demonstra- tion of, 192 staining of, 192 Cervix uteri, glands of, 154 Chains, Graafian, 147 Chamois leather, 34 Change, retrograde, 20 Channels, lymph, 161 spleen, 173 Chinks, lymphatic, 161 Chloroform, Squibb's, 197 Chromatic aberration, 2 Chromic acid hardening, 21 acid fixing, 21 Chyle receptacle, 161 Chylopoietic viscera, 106 Cicatricial tissue, 146 Ciliated cells from oyster, 45 columnar cells, 45 Circulation, lymphatic, 161 Cirrhotic liver, cells from, 52 Cleaning cover-glasses, 31 slides, 31 Clefts, lymphatic, 16: Clove oil, 28 oil, removal o-i, 33 Coarse adjustment, 1 Coiled tubular glands, 154 Collecting tubule, 124 Collodion, 24 Color in air-bubbles, 36 Coloring drawings, 9 Colorless blood-corpuscles, 49 Colors, aniline, 198 oil, 198 Column of Goll, 187 Columnar cells, 44 Columns, postero-external, 185 cortical, 120 postero-internal, 185 Commissure, anterior gray, 185 posterior gray, 186 white, 185 Compound acini, 155 acinous gland, 157 Concavity of blood-discs, 48 Condenser, 4 Abbe", 4 Conducting portion of nerves, 180 Conductor, electrical, 181 Coniferous wood, 38 208 INDEX. Connective-tissue corpuscles, 51 Connective tissue of liver, 106 tissue of lung, 99 tissue of nervous sys- tem, 182 tissue of pancreas, 159 tissue of parotid gland, 157 tissues, 51 tissues, special, 61 Conservation of vision, 7 Constrictions of Ranvier, 181 Contractile rods, 63 Convoluted tubule, 122 Cooper's glue, 198 Copper, acetate, 200 sulphate of, 22 Cords, follicular, 1G7 Cord, spinal, 185 Corium, 69 basement membrane of, 71 Cork support, 19 Corn starch, 38 Cornu of spinal cord, 185 Corpuscles, blood, 203 blood, fixing of, 202 bone, 59 cartilage, 56 connective-tissue, 51 HassaFs, 179 lymph, 174 pus, 49 salivary, 37, 41 tactile, 71 Corpus luteum, 144, 146 Cortex of cerebellum, 194 of kidney, 120 of thymus body, 178 Cortical columns, 120 Corundum hones, 1 7 Cotton fibres, 37 Cover-glass, 31 Cover-glass, pressure of, 36 Covering cells, 51 Crossed pyramidal tracts, 185 Crusta petrosa, 80, 81 petrosa, lacunae of, 80 Crypts of LieberkUlm, 89 Crystals, fat, 55 Cul-de-sac, vaginal, 136 Currents, thermal, 36 Cuticula, 78 Cylinder, axis, 180 Dammar, mode of using, 33 varnish, 184 Decalcificatiori. 61 of teeth, 22, 80 Decalcifying of bone, 22 Degeneration, fatty, 155 Dehydrating tissues, 28 Deiter's cells, 183 Dentinal canals, 78, 82 elements, 82 fibres, 78, 82 sheath, 82 strite, 82 Dentine, 78, 81 Deposits, urinary, 135, 143 Derma, 69 basement membrane of, 71 Descending limb of Henle, 123 Development of ovum. 147 Diameter of kidney tubes, 122, 123 Diaphragm, central tendon of, 163 Differentiation of cell elements, 203. Disc, HensenX 63 Discs, intervertebral, 57 of Bowman, 65 Discus proligerus, 146 Dissociating fluids, 9 process, 22 Distal convoluted tubule, 123 Distilled water, 163 Distribution, cell, 40 Direct cerebellar tracts, 185 pyramidal tracts, 185 Division of cells, 201 Dog, kidney of, 127 stomach of, 86 Double staining, 29, 31 Drainage, tissue, 161 Duct, hepatic, 106 lymph, 90 pancreatic, 159 papillary, 124 Ductless glands, 173 Ducts, lymphatic, 161 mesenteric, 162 thoracic, 162 Dura mater, 191 Dust on lenses, 34 Efferent glomerular arteriole, 125 veins of lymph nodes, 167 Ehrlich on cell elements, 203 Elastic cartilage, 57 lamina of blood-vessels, 66 tissue, 52 tissue in bronchi, 98 Elder pith, 13 Electrical conductor, 181 Elements, dentinal, 82 of ganglia, 181 sarcous, 65 structural, 35 structural, of nervous. system, 180 Embryonic tissue, 62 Emery, flour of, 199 Enamel of teeth, 79 prisms, 79 Endomysium, 64 Endoneurium, 182 INDEX. 209 Endothelial cells, 51 Endothelioid cells, 203 Endothelium, 44 of blood-vessels, 06 Eosin solution, 26 staining, 203 Eosinophilous cells, 203 E. P. E. N. formula, 182 Epidermis, 68 Epineuriuni, 182 lymph-spaces of, 182 vessels of, 182 Epithelia, glandular, 50 Epithelial cancer, 179 cells, 51 Epithelium, buccal, 41 of bladder, 142 of ovary, 148 of tongue, 41 pavement, 42 proliferation of, 135 stratified, 41 squamous, 41 tessellated, 42 transitional, 41 uterine, 136 vaginal, 136 Ether freezing, 16 Eustachian tube, 58 Extraneous substances, 37 substances, mounting of, 38 Eye-lens, 3 Eye-piece, 1 Fasciculi, primitive, 64 Fat-columns of Satterthwaite, 70 Fat-crystals, 55 Fat-globules in milk, 35 in suprarenal cap- sule, 152 Fat-tissue, 54 Fatty degeneration, 155 Fauces, 158 Feathers, 38 Fenestrated membrane, 66 Fermentation spores, 38 Ferrein, pyramids 'of, 122, 124 Fibres, cotton, 37 dentinal, 78, 82 linen, 37 nerve, 180 perforating, 59 silk, 37 wool, 37 Fibroblasts, 51 Fibro-cartilage, 56 Fibrous tissue, 51 Field lens, 3 of view, 5 Fin, caudal, 201 Fine adjustment, 1 14 Fishes, blood of, 48 Fissure, anterior median, 185 posterior median, 185 transverse, of liver, 106 Fixing blood elements, 202 chromic acid, 21 fluid, Flemming's, 201 Flat cells, 41. Flour of emery, 199 Fluid, artificial gastric, 22 dissociating, 9 Flemming's fixing, 201 lymphatic, 161 preservative, 199 Focal adjustment, 5 Focussing, 1, 5 Foetal life, 147 lung, 103 ovary, 148 teeth, 80 Folia of cerebellum, 194 of suprarenal bodies, 150 Follicle of hair, 71 Follicles of Lieberkiihn, 91 solitary, 90 of thymus body, 177 Follicular cords, 167 Foramen dentium, 78 Form of objects, 35 Formulae, miscellaneous, 197 Fourth ventricle, 186 Freezing attachment, 16 Fresh tissue, 20 Frog, bladder of, 62 Frog's mesentery, 42 Funiculus cuneatus of spinal cord, 187 gracilis of spinal cord, 187. Gage, Professor, on Japanese paper, 31 Gall-duct, 107 Ganglia, nerve, 181 of brain, 191 Ganglion cells, 181 cells, poles of, 181 elements of a, 181 Gastric fluid, artificial, 22 juice, 83 tubules, 83 Gaule, "Professor, 202 Gelatinous substance, 185 Genitp-urinary tract, 135 Germinal spot, 146 vesicle, 146 Gill plates, 201 Gland alveoli, 155 mammary, 155 parotid, 156 sebaceous, 73 serous, 159 210 INDEX. Gland sublingual, 157 subrnaxillary, 158, 160 sudoriferous, 71 thymus, 177 Glands, 153 acinous, 155 agminate, 91 branched tubular, 154 bronchial, 96, 154 Brunner's, 89 buccal, 157 coiled tubular, 154 ductless, 173 essentials of, 153 gastric, 154 intestinal, 154 mucous, 158 mucous of bronchi, 98 of cervix uteri, 154 parenchyma of, 153 peptic, 83 Peyer's, 154 pyloric, 84 salivary, 153, 157 sebaceous, 155 sudoriferous, 154 uterine, 154 vessels of, 153 Glandular cells, 50 epithelia, 50 structures, staining of, 159 Glassy membrane of hair, 71 Glisson's capsule, 106 Globules, oil, 36 •Glomerulus of kidney, 125 Glue, Cooper's, 198 Le Page's, 198 Glycerin iri honing, 17 in mounting, 128 Goll, column of, 187 Goose-flesh, 74 Graafian chains, 147 follicles, 144 Granules, pigment, 152 Gray commissure, 185 matter of brain, 191 nerve matter, 181 Gum, dammar, 197 mastic, 197 Hsematoblast, 202 Hsematoxylin, 25 and eosin, 29 staining fluid, 25 staining process, 27 Weigert's, 200 Hair, 37, 71 cortex of, 91 follicle, 71 follicle, muscle of, 74 glassy membrane of, 71 Hair, from shaving, 74 inedullated, 71 permanent mounting of, 74 root-sheath of, 71 transverse section of, 71 Hardening, alcohol, 20 bayberry tallow, 22 by freezing, 24 chromic acid, 21 of bladder, 135 of brain, 192 of foetal ovary, 147 of genito-urinary or- gans, 135 of kidney, 127 of liver, 110, 113 of tissues, 20 of nerve fibre, 180 of os uteri, 135 of ovary, 144 of pancreas, 159 of parotid gland, 159 of small intestine, 92 of spinal cord, 186 of spleen, 174 of sub maxillary gland, 159 of suprarenal capsule, 150 of thymus body, 177 of ureters, 135 of uterus, 135 quick, 21 with Miiller's fluid, 22 Hartnack objectives, 4 Hassal's corpuscles, 179 Haversian canals, 59 system, 59 Hayem's hsematoblast, 202 Heitzmann, Dr. Carl, 61 on development of teeth, 80 Henle, loop of, 123 tubule of, 123 Hensen's middle disc, 63 Hepatic artery, 107 blood-supply, 106 cells, 109 cells, teased, 113 duct, 106 lobules, 106 veins, 106 Hilum of kidney, 120 Hone, 17 water of Ayr, 17 Horns of ganglion cells, 181 Horny layer of skin, 68 Hyaline cartilage, 55 Hydrocarbons, 88 Hydrochloric acid, 22 Ileum, section of, 92 INDEX. 211 Illumination, 4 Imbedding with ailantus pith, 13 with paraffin, 13 Incremental lines, 79 Infiltrating with bayberry tallow, 22 Infiltration with celloidin, 23 Infundibula of kidney, 120 pulmonary, 101 Injection of lung, 101 Insects, 38 Intima of arteries, 66 Interglobular spaces, 78, 82 Interlobular septa of lung, 100 veins, 106 vessels of kidney, 124 Internal elastic lamina, 66 Intervertebral discs, 57 Intestinal lymphatics, 90 villi, 88 Intestine, coats of, 88 of rabbit, 45, 92 small, 88 Invertebrates, blood of, 48 Involuntary muscle, 63 Japan, black, 198 wax, 201 Japanese paper, 31, 34 Juice, gastric, 83 Karyokinesis, 201 Kidney, 120 afferent vessels of, 129 arterial arcade of, 129 blood-vessels of, 124 boundary region of, 144 Bowman's capsule of, 129 calyces of, 120 capsule of, 120, 129 collecting tubes of, 132 convoluted tubes of, 129 cortex of, 120 cortical labyrinths of, 129 diagram of, 121 efferent vessels of, 129 Ferrein's pyramids of, 129 glomerulus* of , 130 hardening of, 127 Henle's limb, 130 Henle's loop of, 130 hi him of, 120 infundibula of, 120 interlobular blood-vessels . of, 129 intertubular capillaries of, 144 labyrinths of, 129 lymphatics of, 120 Malpighian bodies of, 129 Malpighian pyramids, 129 markings, 129 Kidney, medullary portion of, 133 medullary radii of, 129 papillary ducts of, 134 pelvis of, 120, 140 principal tubes of, 134 scheme of, 121 spiral tubes of, 132 tubes, 122 tubules, diagram of, 123 tubules of, 154 Klein on adenoid tissue, 167 on neuroglia, 195 Knives, sharpening, 16 Krause's line, 63 Labels for slides, 34 Laboratory microscope, 2 microtome, 15 Labyrinth, kidney, 122 Lacteals, 90 Lacunae, 58 of crusta petrosa, 80 Lamellae, bone, 59 Lamina, internal elastic, 66 Laminae of crusta petrosa, 80 Larvae of salamander, 201 Lateral tracts, 185 Layers of cerebrum, 192 of epidermis, 68 Lenses, cleaning soiled, 34 water, 36 Le Page's glue, 198 Leucocythaemia, spleen in, 176 Lieberkilhn, crypts of, 89 Life, foetal, 147 Lifting sections, 27 Ligamentum nuchae, 53 -. Light, transmitted, 10 Limb of Henle, 123 Limiting membrane, 39 Line, Krause's, 63 Purkinje's, 82 Linen fibres, 37 for optical surfaces, 34 Lines, incremental, 79 of Retzius, 80 Lining cells, 51 of stomach, 83 Links, Graafian, 147 Liquor potassae, 26 Liver, 105 connective tissue of, 106 lobules of, 106 of pig, 116 parenchyma, 108, 116 physiological scheme, 109 practical demonstration, 109 scheme of structure, 106 Lobes of thymus body, 177 Lobular parenchyma of liver, 108 Lobule, primary, 100 212 INDEX. Lobules, hepatic, 106 of thynius body, 177 Loop of Henle, 123 Lung, 94 connective tissue of, 99 foetal, 103 hardening of, 103 injected, 102 interlobular septa, 100 of pig, 103 parenchyma of, 100 staining of, 103 vascular supply of, 100 Lymph, 161 cavities of brain, 191 ducts of intestine, 90 node, diagram of, 168 node, practical demon- stration of, 169 nodes, 91, 167 nodes, capsule of, 167 nodes, sectioning, 169 nodes, staining, 169 nodes, trabeculae of, 167 nodes, vascular system of, 167 paths, 164 paths, valves of, 164 spaces in portal canals, 116 spaces of nerves, 182 spaces of perineurium, 182 spaces of spinal cord, 190 spaces of thy mus body, 178 Lymphatic capillaries, 161 ducts, 161 system, 161 Lymphatics of intestine, 90 of kidney, 120 peri vascular, 162, 190 Lymphoid cells, 61, 161 Magnification of movements, 36 Magnifying power, 7 Malpighian bodies of spleen, 173 Malpighii, pyramids of, 120 Mammary gland, 155 Markings, kidney, 129 Mastic, 197 Measurement of objects, 7 Media of arteries, 66 Medico-legal work, 103 Medulla, 186 of hair, 71 of thymus body, 178 Medullated nerves, 180 Meisser's plexus, 91 Membrana granulosa, 146 propria of kidney tube, Membrane, basement, 99 basement of corium,71 fenestrated, 66 Membrane, glassy, 71 Limiting, 39 peridental, 81 tubular, 181 Membranes of brain, 191 Menopause, 144 Merck's hflematoxylin, 25 Mesenteric ducts, 25 Mesentery, silvered, 42 Method in observation, 6 Weigert's, 186 Micrometers, 8 Microscope, 1 adjustment, 4 care of, 34 sketching from, 8 Microtome, laboratory, 15 Schrauer, 14 Stirling, 12 the author's, 15 Milk, fat-globules in, 35 Mirrors, 4, 5 Miscellaneous formulae, 197 Molecular movement, 37 Mordants, 30 Morphology, Dr. C. Heitzrnann's, 80 Mounting objects, 31 extraneous substances, 38 varnish, 197 Mounts, rings on, 34 Movement, Brownian, 36 molecular, 37 vital, 37 Movements, magnification of, 36 Mucosa of bronchi, 98 of pelvis of kidney, 140 of small intestine, 88 of stomach, 83 of ureter, 141 of uterus, 136 of vagina, 136 Mucous glands, 158 glands of bronchi, 98 Mliller, capsule of, 122 Miiller's fluid, 22 fluid, hardening with, 22 Multinucleated cells of thymus body, 179 Multipolar nerve cells, 181 Muscle, 62 cardiac, 65 from tongue, 65 non-striated, 62 of hair-follicle, 74 striated, 63 Muscular coat of bronchi, 98 Muscularis mucosse of stomach, 83 Nerve cells, 50, 181 cells, bipolar, etc., 181 IXDEX. 213 Nerve functioning, 181 ganglia, 181 spinal, 185 staining, Weigert's, 200 • trunks, connective tissue of, 181 Nerves, conducting portion of, 180 of special sense, 181 Network, mtracellular, in hepatic cells, 117 Neurilemma, 180 Neuroglia, 01, 181, 186 Klein on, 194 Nigrosin, 203 Nitrate of silver solution, 26 Nodes, lymph, 167 of Ranvier, 181 Non-medullated nerves, 181 Non-striated muscle, 62 Normal salt-solution, 180, 199 Nuclei of cells, 39 Nucleoli of cells, 39 Objectives, 1 Objects, form of, 35 movement of, 36 Odontoblasts, 78, 81 Oil-colors, 198 Oil-globules, 36 Oil of bergamot, 27 of cloves, 28 Omentum, 55 vessels from, 67 Optical axis, 5 Optician's rouge, 199 Organisms in urine, 37 Organs, urinary, 135 Origin of bile-ducts, 118 Osmic acid, 49 acid solution, 200 Osier, Professor, 58 Os uteri, 136 Ova, 146 Ovary, 144 blood-supply of, 149 corpus luteum of, 144 Graafian follicles of, 144 practical demonstration, 144 Ovum, development of, 147 Ox, spinal cord of, 186 Oxygenatipn of blood, 100 Oyster, ciliated cells from, 45 Pancreas, 153 practical demonstration of, 159 Paper, Japanese, Prof. Gage on, 34 Papillary ducts, 124 eminence, 120 Paraffin cement, 11 imbedding:, 13 Parraffin soldering, 18 Parenchyma, 51 of glands, 153 of liver, 108, 116 of lung, 100 Parotid gland, 156 gland, parenchyma of, 159 practical demonstration of, 159 Paste, razor-strop, 199 Pa,tch, Peyer's, 91 Pavement epithelium, 42 Pelvis of kidney. 140 Pepsin, 22 Peptic glands, 83 Perforating fibres of Sharpey, 59 Perichondrium, 96 Peridental membrane, 81 Perimysium, 64 Perineurium, lymph-spaces of, 182 Periosteum, 59 Peripheral nerve termini, 183 Peritoneum, 162 of stomach and intes- tines, 83 Perivascular lymphatics, 162, 190 lymph-spaces of cere- brum, 193 Peyer's patch, 91 Phrenic artery, 150 Pia mater, 191 Picric acid solution, 26 Pig, bronchus of, 45 kidney of, 127 liver of, 116 spinal cord of, 186 Pig's lung, 113 Pigment in suprarenal capsule, 152 Plates, blood, 48 cartilage, 98 Pleura, 100, 162 Plexus, Auerbach's, 86, 91 Meissner's, 91 Pencilling of serous surfaces, 162 Polar cells, 47, 50 Poles of ganglion cells, 181 Pollen, 39 Polyhedral cells, 49 Portal canal, 108 vein, 106 Posterior commissure, 185 Postero-external columns, 185 Postero-internal columns, 185 Potassium ferridcyanide, 200 Potato starch, 38 Power, magnifying, 7 Practical demonstration. Bron- chus of pig, 97 demonstration. Cere- bellum, 196 demonstration. Cere- brum, 192 214 INDEX. Practical demonstration. Devel- opment of ovary, 147 demonstration. Human liver, 113 demonstration. Human lung, 105 demonstration. Intes- tine, 92 demonstration. Kidney, 140 demonstration. Liver of pig, 109 demonstration. Lym- phatics of central ten- don of diaphragm, 168 demonstration. Mesen- teric lymph node, 169 demonstration. Ovary, 144 demonstration . P a n - creas, 159 demonstration. Parot- id gland, 159 demonstration. Spinal cord, 186 demonstration. Spleen, 173 demonstration. Stoii-- ach, 87 demonstration- Sub- maxillary gland, 159 demonstration. Supra- renal capsule, 150 demonstration. Teeth, 81 demonstration. T h y - mus body, 177 demonstration. Uri- nary bladder, 141 demonstration. Ureter, 140 demonstration. Uterus, 138 demonstration. Vagina, 138 Preservative fluid, 199 Pressure of cover, 36 Prickle cells of skin, 69 Primitive fasciculi, 64 Primordial cell, 147 Prismatic color in air-bubbles, 36 Prisms, enamel, 79 Process, decalcifying, 22 dissociating, 22 Processes of odontoblasts, 82 Proliferation, cell, 40, 201 Protoplasm, 89 Proximal convoluted tubule, 122 Pulmonary alveoli, 100 artery, 99 infundibula, 101 Pulp of teeth, 78 Pulp of spleen, 173 Purkinje, cells of, 194 granular layer of, 79 Purkinje's line, 82 Pus-corpuscles, 49 Pyloric glands, 84 Pyramidal tracts, 185 Pyramids of Ferrein, 122 of Malpighii, 120 Quadripolar nerve cells, 181 Quick hardening, 20 Rabbit, central diaphragmatic ten- don of, 163 intestine. 45, 92 kidney of, 127 spinal cord of, 187 Ranvier's nodes, 181 Rapid hardening, 21 Razor stropping, 18 strop paste, 199 Receptaculum chyli, 91, 161 Red blood-corpuscle, 47 muscle, 63 Renal artery, 124, 150 Reptiles, blood of, 48 Reservoir, lymph, 161 Rete Malpighii of skin, 69 mucosum of skin, 69 Reticular cartilage, 57 Reticulum, 40 adenoid, 167 Retrograde changes, 20 Retzius, lines of, 80 Ringing mounts, 34, 198 Rods, contractile, 63 Rogers' stage micrometer, 8 Root-sheath of hair, 71 Rouge, optician's, 199 Sacs, air, 100 Salamander, 201 Salivary abdominal gland, 154 corpuscles, 37, 41 glands, 157 Salt solution, normal, 199 Salter, incremental lines of, 79 Sarcolemma, 63 Sarcous elements, 65 Satterthwaite, fat-coluinns of, 70 Scalp, hair from, 74 Schrauer microtome, 14 Schwann, white substance of, 180 Sebaceous gland, 74, 155 Sebum, 74 Secretion of succus entericus, 88 pancreatic, 159 Section cutting, 9 cutting, free-hand, 10 cutting, with Stirling mi- crotome, 12 INDEX. 215 Section lifter, 32 needle, 27 spoon, 32 Sediments, urinary, 199 Septa, interlobular, of lung, 100 Septum narium, 56 Serous gland, 159 surfaces, pencilling of, 163 Sharpening knives, 16 Sharpey's fibres, 59 Sheath, dentinal, 78 Sheep, spinal cord of, 187 Shellac varnish, 198 Silk fibres, 37 Silver, albuminate of, 164 nitrate of, 26 solution, 26 staining solution, 200 Silvered mesentery, 42 Skeletal muscle, 63 Setching from microscope, 8 Skin, 68 chamois, 34 practical demonstration, 74 staining of, 74 sudoriferous glands of, 72 Slides, labelling, 34 Small intestine, 88 Smooth muscle, 62 Soda, acetate of, 199 Soldering with paraffin, 18 Solitary "glands," 91 lymphatics, 88 Solution, chromic-acid, 22 eosin, 26 normal salt, 180 osmic-acid, 200 picric-acid, 26 silver, 26, 200 Space, subarachnoid, 191 subdural, 191 Spaces, interglobular, 78, 82 venous, 173 Special connective tissues, 61 sense, nerves of, 181 Specimen, permanent, 33 trays, 34 Spheroidal cells, 47 Spider cells, 183 Spinal cord, 185 cord, central canal of, 186 cord, commissures of, 185 cord, diagram of, 185 cord, f uniculus cuneatus of, 187 cord, f uniculus gracilis of, 187 cord, lymph-spaces of, 190 cord, nerve roots of, 185 cord of domestic animals, 187 Spinal cord, practical demonstra- tion, 186 cord, physiological areas of, 185 cord, substantia gelatinosa of, 185 nerve, 185 Spiral tubule, 122 Spirals from tea leaf, 38 Spleen, 173 Malpighian bodies of, 173 practical demonstration of, 173 pulp, 173 supernumerary, 174 Spoon, section, 32 Spores, fermentation, 38 vegetable, 38 Spot, germinal, 146 Squamous epithelium, 41 Squibb's chloroform, 197 Stage micrometer, 8 Staining agents, 25 carmine and picric acid. 31 double, 21, 29 eosin, 203 fluid, borax-carmine, 26 fluid, hsema., 25 fresh tissue, 25 hsema., 27 hsema. and eosin, 27 nigrosin, 203 osmic acid in nerve tissue, 180, 200 silver, 163 solution, silver, 200 Weigert's nerve, 200 Starch, 38 Stars of Verheyen, 126 Stellate cells, 47, 50 sternum, 163 Stirling microtome, 12 Stirling's processes, 22 Stomach, 83 and intestines, 83 hardening of, 87 of dog, 86 Stoniata, 44, 67, 164 Straight kidney tubule, 124 Stratified epithelium, 41 Stratum corrieum of skin, 68 granulosum of skin, 68 lucidum of skin, 68 Striae, dentinal, 82 Striated muscle, 63 Stroma of ovary, 144 Stropping knives, 18 Structural elements, 35 Structure, glandular, 157 Subarachnoid space, 191 Subcutaneous cellular tissue, 70 216 1XDKX. Subdural space, 191 Sublingual gland, 157 Sublobular veins, 100 Submaxillary gland, 158 Substance, white, of Schwann, 180 Substances, extraneous, 87 Substantia gelatinosa, 185 Succus entericus, 88 Sudoriferous gland, 73, 154 Sulci, 191 Sulphate of copper, 22 Supernumerary spleen, 174 Suprarenal bodies, 120 capsule, 150 capsule, practical de- monstration of, 150 Sweat-glands, number of, 74 Sweat -tubes. 154 Sympathetic system, 181 System, cerebro-spinal, 180 Haversian, 59 lymphatic, 161 sympathetic nervous, 181 the nervous, 180 vascular, of kidney, 125 Tactile corpuscles, 71 Tailed cells from ureter and pelvis of kidney, 140 Tallow, bayberry, 22 Tea leaf, 38 Teased nerve fibres, 180 Teasing, 9 glandular structures, 159 Teeth, 78 decalcification of, 80 development of, 80 foetal, 80 grinding sections of, 80 practical demonstration of, 80 Telegraphic cable, 181 Tendon, 51 diaphragmatic, 163 Terminal bronchi, 97, 101 Termini, nerve, 181 Tesselated epithelium, 42 Thermal currents, 36 Thoracic cavity, 162 duct, 162 Thymus body, hardening of, 177 body, Hassal's corpuscles in, 179 Tissue, adenoid, 61, 167 adipose, 54 adipose in bronchi, 99 areolar, 51 cicatricial, 146 connective, 51 connective, of nerve trunks, 181 drainage, 161 Tissue, embryonic, 62 fat, 54 fibrous, 51 fresh, 20 hardening of, 20 muscular, 62 sustentacular of brain, 183 white fibrous, 51 yellow elastic, 52 Tissues, dehydration of, 28 selective power of, 25 special connective, 61 traiislucency of, 28 Tongue, 158 epithelium of, 41 Tooth, canine, 81 Tortuosity of hepatic cell-columns, 115 Trabeculse of lymph nodes, 167 splenic, 173 Trachea, 56, 158 Tract, crossed pyramidal, 183 genito-urinary, 135 Tracts, direct cerebellar, 185 direct pyramidal, 185 lateral, 185 Transitional epithelium, 41 Transmitted light, 9 Transverse fissure of liver, 106 Trigone, 142 Tripolar cells, 50 nerve cells, 181 True skin, 69 Tube, bronchial, 94 Eustachian, 58 Tubular glands, 153 membrane, 181 Tubule, Bellini's, 124 collecting, 124 gastric, 83 Henle's, 123 proximal convoluted, 122 spiral, 122 straight, 123 Tubules, uriniferous, 121 Tumors, 201 Tunica albuginea, 144, 147 Turpentine, 197 Typical artery, 66 cell, 39, 146 Unipolar cells, 50 nerve cells, 181 Ureter, 120 muco«a of, 141 Urinary casts, preserving, 199 deposits, 135 organs, 135 Urine, bacteria in, 37 cells in, 143 course of, in kidney, 122, 126, 127 1X1) EX. 217 TJriniferous tubules, 122 Uterus, mucosa of, 136 Vaeuolated cells, vaginal, 136 Vagina, cul-de-sac of, 136 inucosa of, 136 Valves of lymph paths, 164 Variation of cell forms, 40 Varicosities of nerves, 181 Varnish, asphaltum, 198 black, 198 dammar, 197 shellac, 198 Vascular supply of lung, 100 supply of lymph nodes, 167 system of spleen, 173 system of thymus body, 179 Vegetable spores, 38 Vein, bronchial, 98 portal, 106 Veins, 66 central, 106 efferent, of lymph nodes, 167 hepatic, 106 interlobular, 106 intralobular, 106 sublobular, 106 Venous spaces of spleen, 173 Venulae rect<e. ] 26 Venules, 66 Verheyen, stars of, 126 Vesicle, germinal, 146 Vesicles, air, 100 A7i Hi of intestine, 88 Viscera, chylopoietic, 106 Vital movements, 37 Voluntary muscle, 63 Wall, cell, 39 uterine, 136 Walls of stomach and intestines, 83 Water, boiled, 163 distilled, 163 lenses, 36 of Ayr hone, 17 Wax, bayberry, 22, 201 Weigert's nerve-staining, 186, 200 Wood, coniferous, 38 Wenham objective, 2 Wheat starch, 38 White blood-corpuscles, 49 commissure, 185 fibrous tissue, 51 matter of brain, 191 substance of Schwann, 180 Xiphoid appendix, 58 Xylol, balsam, 197 Yellow elastic tissue, 52 Zinc cement, 198 Zona pellucida, 146 vasculosa, 144 14 DAY USE RETURN TO DESK FROM WHICH BORROWED 6NNJNSV UWH This book is due on the last date stamped below, or on the date to which renewed. Renewed books are subject to immediate recall. AUG 15 1963 2r m r,fen\/ \, I Ala 00 A LD 21-50m-6,'59 (A2845slO)476 General Library University of California Berkeley M THE UNIVERSITY OF CALIFORNIA LIBRARY. 