None
Haffkine, cholera vaccine, 165 plague prophylactic, 145 Halzoun, 370 Hanging drop, 13 Hanging block cultures, 181 Hayem's blood diluent, 251 Helminthic diseases, 314, 366 Hemokonia, 288 Heredity, 239 Herpetomonas, 345 Heterogenesis, 310 Heterophyes heterophyes, 372 Hirudo medidnalis, 410 Hirudo nilotica, 410 Hiss' capsule stain, 56 Hiss' serum-water, 347 Histoplasma, 347 Hodgkin's disease, 300 Hog cholera virus, 532 Homology, 310 Hookworms, 402 Hoplopsylius, 430 Horse asthma, 239 Hosts, 310 Hot-air sterilizer, 8 Howell's bodies, 278 Human complement in Wassermlum, 228 Hydatid disease, 385 Hydrocele agar, 39 Hydrogen dioxide, 595 Hydrogen ion concentration, 28 Hymenolepis, nana, 383 diminuta, 384 murina, 383 Hypersensitiveness to poUens, 239 Hyperthyroidism, 612 Hyphomycetes, 175. Hypoderma diana, 438. Ice box technique, 226 Ichthyotoxismus, 466 Idiosyncrasy, 239 Illumination, dark ground, 7 Immersion objectives, 3 Immune bodies, 199 Immune sera, antimicrobic, 199 antitoxic, 199 diphtheria, 129 in diagnosis, 201 preparation, 200 tetanus, 106 Immunity, active, 197 natural, 196 passive, 198 Inactivation. of serum, 200 Incubators, body temperature, 18 chicken, 18 electrical, 18 petroleum lamp, 18 room temperature, 18 Wassermann, 223, 567 Index, color, 276 ,. Index, malarial, 356 Indican in urine, 582 Indicators, 28, 31, 37 Indol, test for, 32 Infections, focal, 516 of war wounds, 515 infestation, 307 Influenza, 135 Infusion, meat, 24 624 INDEX Infusoria, 349 Inoculation animals (tuberculosis)^ 113 animals (plague), 142 of media, 61 Insecticides, 591 Inspissators, 14 Insecta, 423 Intermediate host, 310 Intestinal amoebse, 348 flagellates, 348 m3riases, 440 Iodine cysts, 327 lodine-eosin stain (amoeba), 58 lodophilia, 264' Isospora bigemina, 353 Itch mite, 415 Ixodidae, 418 Japanese river fever, 543 Jaundice, infections, 334 Joints, gonococcus in, 86 Kaiserling solution, 557 Kala azar, 343 Kedani mite, 414 Key to branching, curving bacilli, 112 to cocci, 73 to filarial embryos, 396 to fleas, 430 to Gram-negative bacilli, 133 larvae in myiases, 442 to spirilla, 162 to spore-bearing bacilli, 93 Kidney diseases, table, 498 Kidney, functioning, 494 Koch's postulates, 71 Koch- Weeks bacillus, 136 Kundrats lymphosarcoma, 300 Laboratory desks, 9 Lactic-acid bacteria, 191 Lactic-acid, gastric, 588 Lactophenol, 556 Lactose excretion, 496 Lamblia intestinalis, 349 Lamp, primus, 22 Lange's colloidal gold test, 522 Large mononuclear increase, 292 Larvae, fly, 441 key to dipterous, 442 mosquito, 449 mounting, 555 Leeches, 410 Leishmania, 343 donovani, 343 infantum, 346 media for, 47 tropica, 343 Leishmaniases, 343 Leprosy, 120 cultural questions, 121 diagnosis of, 122 in rats, 122 Leptomonas, 345 Leptospira icterohaemorrhagiae, 334 Leptothrix, 167 Leukaemia, 297 lymphatic, 297 splenomyelogenous, 297 Leukocytosis, 290 Leukopenia, 288 Levaditi stain, 551 Leydenia gemmipara, 318 Lice, 424 destruction of, 599 Light in microscopical work, 6 Limnea, 370 Linguatula rhlnari^, 421 Liquefaction of gelatin, 67 Litmus, 37 Liver abscess, 515 Liver flukes, 370 Loeffler serum, 38 Loemopsylla cheopis, 430 Luetin, 336 Lumbar puncture, 521 Lung flukes, 373 Lymphatic leukaemia, 298 Lymphoblasts, 287 Lymphocytes, large, 281 small, 281 Lymphocytosis, 292 Lymphosarcoma, 300 Lyon's blood tube, 20 INDEX 625- Macrocytes, 277 Macrogamete, 355 Macroscopic agglutination, 205 Madura foot, 177 Magnif3dng power, 245 of oculars, 4 Malaria, 355 cultivation, 362 diagnosis of, 358 ^ differential tables, 360 index, 356 life cycle, 355 life history, 354 Romanowsky stain in, 358 Malignant pustule, 95 Mallein, 125 Mallory's amoeba stain, 58 Malta fever, 90 Mansonia, 457 Marchi method, 553 Mast cells, 283 Measles, 535 Megarhininae, 453 Meat poisoning, 155 group of bacteria, 154 toxin of, 15s Mechanical stage, i Media (see culture media), 23 Megaloblast, 279 Megakaryocytes, 287 Melania, 370 Melaniferous leukocytes, 292 Meningococcus, 87 agglutination tests for, 209 carriers of, 90 t3rpes of, 89 Metabolism, basal, 612 Metagonimus, 373 Metagonimus yokogawai, 373 Metastrongylus, 401 Methylene blue, 50 Metorchis truncatus, 372 Mett test for pepsin, 589 Micrococcus, 79 catarrhalis, 90 cinereus, 75 M. Ontogeny, 310 Opisthorchis, felineus, 371 noverca, 371 sinensis, 371 Opsonic power, 230 apparatus in, 231 determination of, 232 Oriental sore, 343 Omithodoros, 418 Oroya fever, 540 Orthorrhapha, 432 Otitis, 475 Ova in faeces, 505 Oxydase granules, 265 Oxygen tension-partial, 71 Ox3ruris vermicularis, 409 Pancreatic tests, 504, 590 Pandy globulin test, 525 Pangonia, 433 Panoptic staining, 58, 552 Paragonimus westermanni, 373 Paraplasma flavigenum, 543 INDEX 627 Parasites animal, key to, 305 Parasites animal, table of, 305 Parasitism, 305 Para-typhoid fevers, 155 Parthenogenesis, 310 Pasteurelloses, 140 Pasteurized milk, 193 Pasteur treatment, 527 Pathological sections, 545 Pebrine, 352 Pediculicides, 599 Pediculoide9 ventricosus, 416 Pediculus capitis, 424 vestimenti, 425 Pellagra, 540 Penidllium crustaceum, 174 Pentastomida, 421 f epsin tests, 589 Peptone, 32 Pernicious anaemia, 294 Peroxidase reaction, 265 Pertussis, 138 Petri dishes, 11, 61 Petroflf's T b. medium, 42 Pfeiffer's phenomenon, 165 Phagocytosis, 230 Phar3mgeal secretions, 476 Phenol coefl5cient, 592 Phenolphthalin test, 270 Phenolsulphonephthalein test, 585 Phenylhydrazin test, 575 Phlebotomus, 444 Phlebotomus verrucarum, 445 Phthirius pubis, 426 Phylogeny, 310 Physaloptera, 402 Physiological normals, 663 Piedra, 179 Pigments, bile, in feces, 502 in urine, 583 malarial, 358 Pinta, 174 Pin worm, 409 Pipettes, bacteriological, 20 capillary bulb, 20 Piroplasmata, 363 Pirquet, von, reaction in T b., 118 Plague, 140 diagnosis of, 145 flea in, 429 pneumonia, 145 prophylaxis, 145 Planorbis, 370 Plasma CO', 273 Plasma cells, 287 Plasmodium falciparum, 359 malariae, 358 vivax, 357 Platinum wire, 16 loops, 16 Platyhelminthes, 366 Plerocercoids, 380 Pleural fluids (cytodiagnosis), 519 Pneumococcus, 8z Pneumococcus types, 82 Pneumonias, 78, 139, 481 serum treatment, 83 Poikilocjrtes, 277 Poisonous arthropods, 464 coelenterates, 469 « fish, 466 snakes, 458 Poliomyelitis, 534 Pollen therapy, 239 Polychromatophilia, 278 Polycythaemia, 293 , Polymorphonuclear leukocytes, 282 Pork tapeworm, 382 Porocephalus constrictus, 422 Potato media, 37 Precipitins, 210 in pneumonia, 211 Proagglutinoids, 204 Proteins, blood, 562 Protista, 310 Protozoa, 317 culture of, 46 discussion of, 318 staining of, 57 Protozoal diseases, 313 Prowazekia, 348 Pseudoleukemia, 300 Psychodidae, 444 Pulex, cheopis, 430 628 INDEX Pulex, irritans, 430 Pulicidae, 428 Pupipara, 432 Pus, cultures from, 514 in urine, 493 tetanus in, 106 Putrefaction, 69 Pyelitis, 486 Pyorrhoea, amoebae in, 328 Pyosis, 80 Pyuria, 493 Pyretophorus, 453 Quartan malaria, 358 Rabies, 527 preservation of dog in, 529 Rainey's corpuscles, 364 Rat-bite disease, 541 Rat hosts, 431 Rats, 431 Ray fungus, 175. Reaction of media, 25, 65 standardization of, 28 Reaction, Wassermann, 214 Reagine of S3rphilis, 215 Receptors, 197 Red blood-cells, counting of, 251 normal, 277 nucleated red cells, 279 polychromatophilia, 278 punctate basophilia, 278 Red cell fragility test, 265 Relapsing fever, 330 Renal efficiency determination, 494 Reservoir virus, 312 Reticulation, red cells, 255, 302 Rhabditis pellio, 389 Rheumatism (acute), 533 Rhinosporidium, 364 Rhipicephalus, 421 Rhizogl3rphus parasiticus, 415 Rhizopoda, 319 Rhizopus, 169 Rhynchota, 426 Rice cooker, 15 Ring worm, 171 Rocky Mountain spotted fever, 535 Roe^heln, 535 Romano wsky stains, 260, Room temperature incubators, 18 Ross thick film, 258 Round worms, 388 Row's haemoglobin medium, 47 Russell's double sugar medium, 45 Russell's viper, 458 Sabouraud's medium for moulds, 181 Saccharomyces, anginosae, 170 blanchardi, 170 cerevisiae, 170 Sahli haemoglobinometer, 24^ Salt retention, 495 Sarcina lutea, 79 Sarcocystis tenella, 364 Sarcodina, 319 Sarcophaga carnaria, 438 Sarcopsylla penetrans, 431 Sarcoptes scabiei, 415 Sarcosporidia, 363 Scarlet fever, 535 Schick reaction, 129 Schilling-Torgau differential count, 285 Schistosomum haematobium, 376 japonicum, 377- mansoni, 377 Schizotrypanum, 342 SchuflFner's dots, 358 Scorpions, 464 Screw worm, 438, 441 Sections, making and staining, 545 Sediment, urinary, 488 Sensitization, 200, 235, 236 Sensitized vaccines, 235 Septicaemia, 509 Serum (see immune serum), 199 Serum broth, Holman's 32 Sewage, in water, 182, 185 Sheep cells, 219 Shiga's bacillus, 151 Simulidae, 443 Siphonaptera, 428 Siphunculata, 424 Skin infections, 517 INDEX 629 Skin infections, itch mite, 415 leprosy in, 518 pus cocci in, 517 sarcopsylla in, 518 Sleeping sickness, 339 Slides, cleaning, 49 concave, 13 Smallpox, 530 Snakes, 458 Sore, oriental, 346 Sparganum, mansoni, 387 prolifer, 387 Spectroscope, 271, 485 Spiders, 464 Spinal fluid, 521 Spirillum cholerae asiaticse, 162 metschnikovi, 162 of Finkler-Prior, 162 tyrogenum, 162 Spirochaeta, 329 duttoni, 330 icterohaemorrhagiae, 334 pallida, 335 refringens, 335 vincenti, 477 Spirochseta plicatilis, 329 Spirochaeta morsus muri, 335 Spirochaete staining, 59 Spironema buccalis, 335 Spiroschaudinnia, 338 Splenic anaemia, 302 Splenomegaly, 301 Spore formation, 67 Spores, spore-bearing bacilli, 93 staining, 57 Sporotrichosis, 179 Sporotrichum beurmanni, 1 79 Sporozoa, 350 Sprue, 542 Sputum, 479 albumin test in, 483 amoebae in, 482 antiformin for, 480 centrifugalization for T. B., 480 culturing, 482 fixing smears, 481 of bronchopneumonia^ 481 Sputum, Paragonimus eggs in, 483 Petroff's T. b. culturing, 42 plague pneumonia, 482 Stage, warni, 5 Staining methods, 49 Stains, acid fast, 52 agar jelly, 254 Archibald's, 53 Balch's, 261 capsule, 55 carbol fuchsin, 50 carmine for worms, 556 flagella, 56 Fontana spirochaeta, 59 for Negri bodies, 529 Giemsa's, 263 Gram's method, 50 haematoxylin, 59, 263, 551 Herman's, 53 Hiss' capsule, 56 iodine eosin, 58 iron haematoxylin, 59 Leishman's, 261 Levaditi's, 551 Loflfler's methylene blue, 50 Mallory's, 58, 59 Neisser's, 54 Nicolle's, 54, 550 panoptic, 58, 552 Pappenheim's, 54 peroxidase, 265 Ponder's diphtheria, 55 protozoal, 57 Romano wsky, 260 Rosenbusch's, 59 Smith's formol fuchsin, 53 spore, 57 Sterling's gentian violet, 51 tri-acid, 260 Van Giesen's, 551 vital, 255 Wilson's, 261 Wright's, 260 Ziehl-Neelsen, 50 Staphylococcus, 79 epidermidis albus, 80 pyogenes albus, 79 630 INDEX Staphylococcus, pyogenes aureus, 79 Starch granules, 492 Steam sterilizer, 10 Stegomyia, 455 Steriginatocystis, 175 Sterilization, Arnold, 10 autoclave, 10 glass ware, 1 1 hot air, 8 Sterilization, pathogenic bacteria, 11, 591 Stomach contents, 511 Boas-Oppler bacillus, 511 cancer cells in, 511 chemical examination of, 587 Stomoxys, 435 Stool examination, 500 Streptococcus, 75 anaerobic, 509 carriers, 78 haemolyticus, 73 lacticus, 191 mucosus, 77 pyogenes, 76 viridans, 76 Streptothrix, 180 Strong, cholera prophylactic, 166 plague vaccine, 145 Strongylidae, 400 Strongyloides stercoralis, 389 Strongylus apri, 401 Sugar in blood, 560 in urine, 575 media, 30, 187 Sulphur disinfection, 594 Susceptibility to parasites, 307 Swabs, II S3^hilis, fixation of complement in diagnosis, 214 Giexxisa's stain in, 335 luetin test, 336 organism of, 335 precipitate tests in, 228 smears in, 336 spinal fluid tests, 215, 525 Wassennani^ reaqtioni 219 Tabanus, 433 Tables, insects, 423 mosquitoes, 453, 455 of arachnoids, 412 of filarial worms, 397 of flat worms, 366 of fungi, 167 of myiasis larvse, 442 of parasitic animals, 305 of protozoa, 317 of round worms, 388 Tables, pressure and temperature, i j urinaxy findings, 498 Taenia, africana, 383 echinococcus, 385 saginata, 380 solium, 382 Taeniorhynchus, 457 Tape worms, adult, 379 somatic or larval, 385 Tarantula, 464 Telosporidea, 350 Terminology, 309 Test breakfast, 587 Test-tubes, 12 Tetanus, 105 antitoxin, 108 chronic, 109 diagnosis of, loi, 108 toxin, 106 Tetragena nucleus, 322 Tetramitus mesnili, 349 Texas fever, 363 Thalman's gonococcus medium, 42 Theileria, 363 Theobaldia, 457 Thick film blood smears, 258 Thionin, 54 Thoma-Zeiss hemacytometer, 249 Thorn-headed worm, 410 Throat examination, 476 Thrush, 178 Tick fever, 330, 418 Ticks, 416 classification of, 412 life history, 417 Tin^a, 1 71; INDEX 631 Tinea, imbricata, 172 versicolor, 177 Tissue, acetone method, 547 chloroform method, 547 dehydration of, 546 fixation of, 545 imbedding, 547 nervous, 552 preparation of for sections, 545 Titration of amboceptor, 215, 218 of complement, 215 Titration of media, 25 Toisson's solution, 251 Tongue worms, 421 Toxascaris, 409 Toxic spit proteids, 238 Toxin, 198 botulism, 103 diphtheria, 128 gas gangrene, 11 1 tetanus, 106 Toxone of diphtheria, 128 Toxoplasma pyrogenei, 347 Trachoma, 536 and Kock- Weeks bacilli, 137 Transfusion bloodtests, 266 Transitional leukocyte, 281 Transmission of parasites, 311 Transudates, 519 Trematoda, 366 Trench fever, 542 Trench foot, 536 Trench nephritis, 536 Treponema, 335 culture media for, 47 pallidum, 335 pertenue, 337 Trichinella spiralis, 399 Trichinosis, 399 Trichocephalus trichiurus, 398 Trichomonas intestinalis, 348 vaginalis, 348 Trichophyton mentagrophytes, 172 Sabouraudi, 171 tonsurans, 171 Trichostrongylus instabilis, 401 Trichuris, 398 Triodontophorus deminutus, 401 Triple vaccine, 149 Trombidiidse, 413 Trombidium holosericeum, 413 Trypanosoma, brucei, 341 equinum, 342 equiperdum, 342 evansi, 342 gambiense, 339 lewisi, 342 nigeriense, 339 of animals, 340 rhodesiense, 340 Trypanosomiasis, 339 Tsetse flies, 340, 436 Tuberculin, 117 Tuberculins, in diagnosis, 118 ' in treatment, 232 Tuberculosis, bacilli of, 114 atrium of infection, 115 avian and fish, 116 blood cultures in, 119, 510 bovine and human, 115 British commission report, 115 Calmette eye reaction, 118 diagnosis of, 119 in guinea-pigs, 113 intradermic test, 119 Much's granules, 53 secondary infections, 117 staining methods, 52 von Pirquet skin reaction, 118 Tubes, fermentation, 14 test-tubes for agglutination, 205 Wright's U-tube, 20, 222 Turck, irritation cells, 287 ruling on haemacytometer, 250 Typhoid, agglutination in, 148, 205, 207, 510 and water supply, 151 blood cultures, 507 carriers, 150 dead cultures, 206 gall stones in, 147 in water, 189 relapses in, 148 serum for, 148 632 INDEX Typhoid, statistics, 151 vaccination in, 149 Typhus fever, 536 Tyroglyphus longior, 415 Ultra microscope, 7 Uncinariasis, 402 Urea, 564, 580 in blood, 564 in urine, 580 index of excretion, 495 Urease tests, 564 Uric acid, in blood, 565 in urine, 583 Urine, 484, 573 albumin in, 573 animal parasites in, 487 bile in, 583 blood in, 492 casts in, 491 chemical examination, 573 chlorides in, 583 chylous, 394, 487 culturing of, 486 diastase test in, 504 epithelium in, 491 haemoglobin in, 493 moulds in, 484 reaction of, 578 red cells in, 492 schistosomum eggs in, 487 sediment in, 4^8 smegma bacillus in, 487 staining sediment, 484 starch grains and fibres in, 492 sugar in, 575 urea in, 580 uric acid in, 583 Urobilin, 582 Urotropin and urine, 584 Urticarial fever, 378 Uta, S18 Vaccines, 232 doses of, 234 i preparation of, 232 sensitized, 235 Vaccines, standardizing of, 234 therapy of, 235 typhoid and parat3T)hoid, 149 Vaccinia, 531 Vacuum bottle, 18 Varicella, S3o»-537 Vedder's starch medium, 35 Vegetable cells in feces, 500 Ver du Cayor, 440 Verruga peruana, 543 Vincent's angina, 478 Viperine snakes, 458 Viscosity of blood, 264 Vital staining, 255 Vitamines, 538, 540 Voges-Proskauer, 32, 188 Von Jaksch anaemia, 302 Von Pirquet T. F. ~-TV_j •? IW - - \io Its 1)0 - ~ 1.4ff 1»^ - I z fct.0 100 - ^- Mk Equivalent Fahrenheit and p. fj. p. " Centigrade tables for the temper- in Ubora- 1. Those for sterilization oi dressings and media. Also foi )^ j^ certain disinfection of spore-bear ing bacterial contaminstioi: (autoclave tempieratures). 3. Those for pasteunzatioi and sterilization of bacterial vac ' cines. Also for paraffin batl (pasteurizing temperatures). 3. Those for growing impor tant pathogenic organisms (bod; ^i.it temperatures). ^ r~— -1 4 5 ? S .^ I 4. Those for culturing gelatii , a a (melting point, 2S°C.) as in wate '— work (room temperatures). 5. Those employed in preserv ' ing biological products and post mortem material; also in centd fuging experiments to separat complement and amboc'epto (freezing temperatures). UNITS IN COMMON USE IN LABORATORIES Cubic Meter. — Unit of space for the niunber of organisms in air. It contains looo liters. It is equal to 1.308 cubic yards or 35.316 cubic feet. One thousand cubic feet, the unit of space in disinfec- tion, is equal to 28.3+ cubic, meters. One cubic decimeter is one liter and equals 0.908 quarts dry measure or 1.0567 quarts liquid measure. Liter. — Unit of space for normal volumetric solutions. It contains 1000 cubic centimeters. It is equal to 1.0567 quarts or 33.8+ ounces. A liter of distilled water weighs i kilogram. One U. S. gallon is equal to 3785 c.c. and one imperial gallon to 4543 c.c. One fluid ounce equals 29.57 c.c. Cubic Centimeter. — Unit of space for organisms in water, milk, vac- cines, etc., I c.c. =0.27 fl. dr. There are, approximately, 20 drops in I c.c. of water, provided the capillary pipette has a bore of about I mm. and is held horizontally. A finely drawn capillary pipette, held vertically, will deliver about 50 drops from i c.c. Cubic Millilmeter. — Unil of space for blood-cells. There are 1000 cubic millimeters in i cubic centimeter and i million cubic milli- meters in I liter. In water analysis, as there are i million milli- grams in one liter, parts in the million and milligrams per liter are the same. I Meter =39.37 inches. I Centimeter =0.3937 inch. Approximately, 2/5 inch. I Millimeter =0.0393 inch. Approximately, 1/25 inch. I Inch = 25.4 mm. I Yard =0.9144 m. I Kilogram = 2.2 +pounds av. I Gram = 15.432 grains. I Centigram =0.154 grain. • I Milligram =0.0154 grain. Approximately, 1/64 grain. A pound avoirdupois is equal to 453.59 gm. I Oz. avoirdupois is equal to 28.35 gm. One hundred cubic centimeters of a saturated solution contains: . \ Water Alcohol Methylene blue, 6.68 0.66 gram. Gentian violet. 1. 75 4 . 42 grams Basic fuchsin, 0.66 2 . 92 grams. Key to Table on opposite page. -:-= negative, H-=positive, 0 = no change, A=acid, Alk.= alkaline, G=gas; Fl= fluorescence, Pep = peptonization, 1= litmus RusseU's medium not reduced; 2= reduced, 3= action variable. Proteus gives a peculiar dark,  1 .a 1 i 1 1 i ■s £ \ .a 1 1 1 i'E 3-3 1 111 % ill |-ea ■Sto ft HI i 11 h 1 1i h r, I'* "I 'Cft it s ii III «nmiKud-K>*>A 1 1 1  ■ B o § i 5 i r <   ■< ■< < < o Mffimjo o < < < •< < O < ■«! ■<! < < < 3 IJ i a < 5 i 3 a «: a 3 a < < < 1 t § S| % < < 3 i o -fl 1 < i a s| ^ < < ^ < o -fl ^  i a || <* 1 n 6 i 1 1 a m i 1 2 I. 