None
structure, 29, 34 thermal death-point of, deter- mination, 300 thermophilic, 64 toxic power, 89 transplantation of, from culture- tube to culture-tube, method of, 245 virulence of, 95. See also Viru- lence. Bacterial suspension in testing op- sonic value of blood, 308 Bactericidal strength of common disinfectants, 217 Bacterination, 113 Bacteriologic syringes, 269 Bacteriology, evolution, 17 of air, 283 of foods, 296 of soil, 294 Index 857 Bacteriology of water, 297 Bacteriolysins, 166 Bacteriolysis, 164, 166 Bacterio-vaccination in staphylo- coccic infections, 349 Bacterium, 40 coli dysenterise, 699 pneumoniae, 507 termo, 369 Bagdad boil, 572 Bain fixateur, 192 reducteur et reinforcateur, 192 sensibilisateur, 192 Balantidium coli, 704 animal inoculation with, 707 cultivation, 707 habitat, 707 lesions produced by, 707 morphology, 705 motility, 706 pathogenesis, 707 reproduction, 706 staining, 706 transmission, 708 diarrhea, 704 lesions of, 707 transmission of, 708 Barber's itch, 827 Beckman's method of isolating typhoid bacillus, 657 Behring's method of determining potency of diphtheria senun, 157 Bench, glass, 248 Bergell and Meyer's typhoid serum, 649 Berkefeld filter, 208 Bichlorid of mercury as germicide, 212 Bile-salt agar-agar, 662 Biologic contributions, 17 Biology of bacteria, 58 Biondi-Heidenhain method of staining protozoa in tissue, 199 Biscra boil, 572 button, 572 Black death, 581 fever, 566 molds, 47 plague, 581 Bladder, bacteria in, 85 Blastomyces dermatitis, 818 cultivation, 821 lesions, 823 pathogenesis, 823 staining, 821 transmission, 823 Blastomycetes, 43 dermatitis, 44 Blastomycetic dermatitis, 818 Blastomycosis, 818 specific organism, 820 transmission, 823 Blenorrhea, 450 Blood agar-agar as culture-media, 234 Bacillus typhosus in, 643, 644 method of securing, from animals, 273 opsonic value of, bacterial sus- pension in testing, 308 serum in testing, 3 1 1 washed leukocytes in test- ing, 311 phagocytic power of, 307 streptococcus in, in scarlatina, 355 Blood-corpuscles for Wassermann reaction, 322 titration of, 324 preparation of, 164 Blood-culture in typhoid fever, 650 Blood-serum, alkaline, as culture- media, 237 as culture-media, 234 mixture, Loffler's, as culttu-e- media, 236 for cultivation of Bacillus diphtheruB, 454 therapy, 26 Boil, Aleppo, 572 bagdad, 572 biscra, 572 Delhi, 572 Bordet-Gengou bacillus, 488 and influenza bacillus, differ- ences between, 491 cultivation, 490 isolation, 489 metabolic products, 490 morphology, 489 pathogenesis, 490 staining, 489 phenomenon, 170 Botkin's apparatus for making anaerobic cultures, 262 Botulism, 299 Botulismus, 69 Bouillon as culture-media, 227 preparation of, from fresh meat, 227 from meat extract, 229 sugar, 230 Bouillon-filtrate, Denys', 742 Bovine actinomycosis, 803 tuberculosis, 7^8 commimicability to man, 750 prophylaxis, 753 tuberculin test for, 754 858 Index Bromatotoxismus, 298 Bronchopneumonia, 512 Broth, nitrate, 74 Brownian movement of bacteria, 36 Buboes in plague, 583 Bubonic plague, 581. See also Plague. Buchner's method of making anae- robic cultures, 263 Buerger's medium for isolating diplococcus pneumoniae, 495, 496 Buret for titrating media, 225 Burri's India ink method of identi- fying treponema pallidum, 792 Buton d'Orient, 572 Butter bacillus, 760 bacteria in, 297 Butyric fermentation, 68 Buxton and Coleman's culture- medium for typhoid bacillus, 661 Cabot's method of treatment of hydrophobia, 419 Calmette's ophthalmo-tuberculin reaction, 741 Canal-water bacillus, capsulated, 508 Canned goods, poisoning from, 299 Capaldi's medium for plating ty- phoid bacillus, 659 Capillary glass tubes, 243, 24^ Capsulated canal-water bacillus, 508 Capsule bacillus, 509 of bacteria, 34 Capsules, collodion, 276 Carbolic acid as disinfectant, 215 Carbuncle, malignant, 401 Carcinoma of stomach, Oppler- Boas bacillus in, 83 Caries, dental, bacteria in, 82 Carrasquilla's leprosy serum, 774 Carriers, typhoid, 640 Catarrhal inflammation, 439 pneumonia, 512 Catgut, sterilization of, 210 Claudius' method, 211 cumol method, 211 Celloidin embedding, 179 Cells, giant-, in tuberculosis, 732 lepra, 772 specific affinity of, for toxins, 93 Cell-walls of bacteria, 34 Ceratophyllus fasciatus, 593 Cercomonas intestinalis, 709 Cerebrospinal fever, 423 meningitis, 423 Cerebrospinal meningitis, diplococ- cus of, 423 Chamberland filter, 208 Chancroid, 442 Chantemesse's ocular typhoid re- action for diagnosis of typhoid fever, 650 Chapin and Adami's method for isolation of typhoid bacillus, 656 Charbon, 400 Cheese-poisoning, 299 Chemic agents, influence on growth of bacteria, 65 contributions, 20 Chester's synopsis of groups of bacteria, 279 Chicken-cholera, 598 Chlamydophrys stercorea, 690 Chlorin as germicide, 214 Cholera, Asiatic, 604 immunity against, 610 prophylaxis, 617 rice-water discharges of, 613 sanitation in, 618 specific organism of, discovery, 605 chicken-, 598 de poule, 598 hog-, 678 nostras, 619 Chromogenesis, 71 Chromogenic bacteria, 71 Chromogens, 66 Cilia of protozoa, 56 Cladothrix, 42 Claudius' method of sterilization of catgut, 2 1 1 Clegg and Musgrave's agar-agar for cultivating amebas, 692, 694 Clegg's method of cultivation of lepra bacillus, 767 Clonic convulsions in tetanus, 393 Clostridium, 37 Clothing, etc., disinfection of, 221 Coagulins, 140 Cobralysin, 120 Cocci, 38 Coccidioidal granuloma, 819 Coccus, diagram illustrating mor- phology, 39 Cold, effect of, on Bacillus typho- sus, 637 influence on growth of bacteria, 65 Coleman and Buxton's medium for typhoid bacillus, 661 Coley's mixture, 358 Collodion capsules, 276 sacs, preparation of, 276, 277 Index 859 Colonies, 246, 251 in Esmarch's tubes, Esmarch in- strument for counting, 288 types of, 251 Colors produced by bacteria, 71 Comma bacillus, 606 Complement, 120, 141, 142, 144, 145 deviation of, phenomenon result of, 167 fixation, 170 for Wassermann reaction, 321 titration of, 324 Complicating pneumonias, 513 Concentrated tuberculin, 739 Congestive chills of malaria, 539 Conjunctiva, bacteria in, 82 Conjunctival reaction in typhoid fever, 650 Conjunctivitis, acute contagious, 446 miscellaneous organisms in, 450 Conorhinus megistus, 565 Conradi-Drigal^i method of iso- lation of typhoid bacillus, 656 Contagion from Bacillus diph- therias, 468 Contagious conjimctivitis, acute, 446 Contractile vacuoles, 54 Convulsions, clonic, in tetanus, 393 tonic, in tetanus, 393 Coplin's staining jar, 181 Copper sulfate as germicide, 213 Corks, sterilization of, 204 Comil and Babes' method of stain- ing spirillum cholerae Asiatics, 608 Corpuscles, blood-, for Wasser- mann reaction, 322 titration of, 324 preparation of, 164 Cover-glass forceps, 177 preparations for general exami- nation, 175, 176 for staining protozoa, 196 Gram's method, 185 Creolin, 215 Croupous pneumonia, 492 lesions, 501 Crude tubercles, 733 tuberculin, 738 Cryptobia borreli, 556 Ctenopsylla musculi, 593 Culex pipiens, 542 Culicidae, classification, 542 Culture-media, 224 agar-agar, 232 alkaline blood-serum, 237 Culture - media, blood -agar -agar, 234 blood-serum, 234 bouillon, 227 Deycke's alkali-albuminate, 237 Dunham's solution, 240 glycerin agar-agar, 234 litmus milk, 239 Loffler's blood-serum mixture, 236 milk, 239 peptone solution, 240 Petruschky's whey, 240 potatoes, 237 potato-juice, 238 standard reaction, 227 sterilization and protection, 205 Culttu-es, 242 agar-agar, 255 anaerobic, Botkin's apparatus for making, 262 Buchner's method of making, 263 Frankel's method of making, 261 Hesse's method of making, 265 Koch's method of making, 266 Liborius' tube for, 261 Nichols and Schmitter's meth- od of making, 263 Novy's jars for, 261 Salomonsen's method of mak- ing, 266 Wright's method of making, 264, 267 Zinsser's method of making, 264 freshly isolated, standardizing, 259 gelatin culture, 254 in fluid media, 256 manipulation, technic, 243 microscopic study, 259 plate, 242, 246 apparatus for, 247 Esmarch's tubes for making, 250 method of, 247 Petri's dishes for making, 249 pure, 242, 252 special methods of securing, 256 shake, 265 standardizing freshly isolated, 259 study of, 242 uiK)n potato, 256 Cumol method of sterilization of catgut, 211 86o Index Cup, pasteboard, for receiving in- fectious sputum, 220 Cutituberculin reaction, 741 Cytase, 144 Cytolysins, 164 Cytolysis, 164 Cytoplasm, 34 of protozoa, 53 Cytotoxins, 163 Czenynke's stain for Bacillus in- fluenzae, 514 Davis' method of cultivation of Ducrey's bacillus, 443 Death, black, 581 Death-point, thermal, of bacteria, determination of, 300 Defensive proteids, 128 Dejecta, disinfection of, 220 Delhi boil, 572 Dematium albicans, 485 Denecke, spirillum of, 622 Dental caries, bacteria in, 82 Deny's tuberculin, 742 Dermatitis, blastomycetic, 818 Dermatomycosis, 824 Dermatotuberculin reaction, 741 Desmon, 144 Deviation of complement, phenom- enon result of, 167 Deycke's alkali-albuminate as cul- ture-media, 237 Diarrhea, balantidium, 704 lesions of, 707 transmission of, 708 Digestive apparatus, infection through, 86 Diluted tuberculin, 739 Diphtheria, 451 antitoxin, 155, 471 bleeding, 156 effect on death-rate, 473 immunization of animals, 156 method of administration, 471 paralysis after use of, 472 potency of serum, 157 preparation of serum, 157 of toxin, 156 prophylaxis, 471 tetanus after, use of, 473 treatment with, 471 bacillus of, 45 1. bacteriologic condition of throat in, 462 contagion from, 468 diagnosis, 456 bacteriologic, 456 Diphtheria, diagnosis, outfit for, 456 lesions, 466 mixed infections, 466 paralysis after, 472 pseudomembrane of, 466 relation of Bacillus diphtherise to, 467 of streptococcus pyogenes to, 354 toxin, 460 DiplobacUlenconjimctivitis, 448 Diplococcus, 38 intracellularis meningitidis, 423 agglutination, 428 cultivation, 426 distribution, 424 identification, 425 isolation, 426 metabolic products, 428 micrococcus catarrhalis and, differentiation, 425 mode of infection with, 430 morphology, 424 meningitidis, pathogenesis, 428 specific therapy with, 430 staining, 425 vital resistance, 427 of Weichselbaum, 492 pneumoniae, 492 animals susceptible to, 502 bacteriologic diagnosis, 503 cultivation, 496 distribution, 493 general characteristics, 492 immune serum against, 505 immunity against, 505 isolatioiki, 495 metabolic products, 498 morphology, 493 pathogenesis, 498 specificity of, 502 staining, 494 toxic products, 498 virulence, 502 vital resistance, 497 Diseases, infectious, 339 study of, 21 production of, 76 Dishes, Petri's, 249 Disinfectants, common, bacterici- dal strength, 217 determination of value, 302 inorganic, 213 organic, 215 Disinfection and sterilization, 201 gaseous, 305 of air of sick-room, 211 of bodies dead of infectious dis- eases, 223 Index 86 1 Disinfection of clothing, etc., 221 of dejecta, 211, 220 of furniture, etc., 222 of hands, 209 of instruments, 209 of ligatures, 209 of patient, 222 of sick-chambers, 211 of sutures, 209 of wound, 2 1 1 use of sulphur in, 218 with formaldehyd, 218 Displacement of air by inert gases in anaerobic cultures, 260 Donovan-Leishman body, 566 Dorset's method of isolation of tubercle bacillus, 723 Dose, amboceptor, in Wassermann reaction, 326 Dourine, 562 Drigalski-Conradi method of iso- lation of typhoid bacillus, 656 Drumstick, 37 Ducrey's bacillus, 442. See also Bacillus of Ducrey. Dumdum fever, 566 Dunham's solution as culttu-e-me- dium, 240 Duval's method of cultivation of lepra bacillus, 767, 768 Dyscrasia, loi Dysentery, 687 amebic, 689 abscess of liver in, 697 lesions, 697 bacillary, 699 diagnosis, 704 lesions, 702 serum therapy, 704 bacillus of, 699. See also Bacillus dysenteries. endemics of, 687 epidemics of, 687 Eberth-Gaffk V bacillus, 632 . See also Bacillus typhosus. Edema, gaseous, 377 bacillus of, 377 See also Bacil- lus aerogenes capsulatus. malignant, 374 bacillus of, 374. See also BclcU- lus cedematis mcUigni. Ehrlich-Koch method of staining tubercle bacillus, 715 Ehrlich's lateral-chain theory of immunity, 131 method of determining potency of diphtheria serum, 158 Ehrlich's method of staining tuber- cle bacillus, 713, 715 in sections, 719 solution, 182 Electricity, influence on growth of bacteria, 62 Electrozone, 214 Elephantiasis graecorum, 771 Eisner's method of cultivation of typhoid bacillus, 652 Embedding, 119 celloidin, 179 glycerin-gelatin, 180 paraffin, 180 Emulsion, bacillary, 745 Encyst ment of protozoa, 57 Endogenous infections, 80 Endomyces albicans, 485 Endo's method of isolation of ty- phoid bacillus, 659 Endospores, 37 Endotheliolysis, 164 Engle and Reichel's stain for Negri bodies, 421 Entamoeba buccalis as cause of suppuration, 372 coli, 691 table of differential features, 693 histologica, 689, 691 morphology, 691 reproduction, 692 staining, 692 table of differential features, 693 lesions produced by, 697 Mallory's differential stain for, 698 metabolic products, 695 pathogenesis, 695 tetragena, 690, 692 isolation and cultivation, 692 metabolic products, 695 pathogenesis, 695 table of differential features, 693 vital resistance, 695 vital resistance, 695 Enteric fever, 632 Enzymes, production of, 77 tryptic, 71 Eosin and methylene-blue stain, 186 Epidemic cerebrospinal meningitis, 423 Epitheliolysins. 121 Epitheliolysis, 164 Ernst-Babes granules, 34 Erysipelas, streptococcus of, 361 862 Index Erythrasma, 824 Esmarch's instrument for counting colonies of bacteria in Esmarch tubes, 288 tubes, 250 Estivo-autumnal fever, parasite of, 537 Eurotium, 48 Exhaustion theory of immunity, 125 Exogenous infections, 79 Experimentation upon animals, 268 Ei^tracellular toxins, 89, 91 Facultativb anaerobes, 59 Farcin du boeuf , 43 Farcy, 781 Farcy-buds, 781 Fatdnisszymoid, 24 Favus, 828 scutulum formation, 828 specific organism of, 829 Febrile tropical splenomegaly, 566 Feces, Bacillus typhosus in, isola- tion, 650 bacteria in, 84, 85 spirillum cholers Asiatics in, Schottelius' method of detect- ing, 609 tubercle bacillus in, staining, 718 Ferment, putrefactive, 24 Fermentation, 20, 67 acetic, 67 alcoholic, 67 butyric, 68 lactic acid, 68 Fermentation-tube, Smith's, 69 Filter, Berkefeld's, 208 Chamberland, 208 Kitasato's, 208 Pasteur-Chamberland, 207 Petri's sand, for air-examination, 285 Reichel's, 208 Filtration, sterilization by, 208 Finkler and Prior spirillum, 619 Fiocca's method of staining spores, 189 Fish tuberculosis, 756 Fishing, 252 Fish-poisoning, 299 Fission, 36 results, 36 Fixateur, 144, 145 Fixation of complement, 170 Fixed virus in hydrophobia, 414 Flagella, 35 staining of, 1 89. See also Stain- ing flagella. Flagellates in intestines, 709 Fleas, plague and, 592, 593 Fleischner-giftung, 69 Flexner variety of dysentery bacil- lus, 702 Flexner 's antimeningococcus serum, 430 Flies, plague and, 592 Fluid media, cultures in, 256 Muller's, 259 Zenker's, 179 Fluorescein, 366 Fomites, 80 foods as, 296 Food as fomites, 296 bacteria in, 296 bacteriology of, 296 influence on growth of bacteria, 59 of molds, 60 of protozoa, 60 of yeasts, 60 poisons, 298 Food-poisoning, 298 Forceps, cover-glass, 177 Petri dish, 249 sterilization of, 204 Formaldehyd, 218 as germicide, 218 Formalin, 215, 218 Fowl tuberculosis, 754 Frambesia tropica, 800 Frankel's instrument for obtaining earth for bacteriologic study, 295 method of estimatmg number of bacteria in soil, 294 of making anaerobic cultures, 261 of staining diplococcus intra- cellularis meningitidis, 425 Friedlander's Bacillus pneumoniae, 507. See also Pneumococcus. Frost's plate counter, 290 Frothy organs, 384 Frugoni's method of isolation of tubercle bacillus, 725 Fungi, ray-, 804 Funnel for filling tubes with culture- media, 230 Furniture, etc., disinfection of, 222 Fusiform bacillus, 478 Gabbbt's method of staining tuber- cle bacillus, 716 Gaffky-Eberth bacillus, 632. See also Bacillus typhosus. Galactotoxismus, 298 Index 863 Gall-bladder, Bacillus typhosus in, 640 Gamaleia, spirillum of, 625 Gametocytes of Plasmodium falci- parum, 538 malarix, 534 vivax, 536 Gartner's bacillus, 675 Gaseous disinfection, 305 edema, 377 Gases, production of, 69 Gastro-intestinal tract as avenue of infection for tubercle bacillus, 728 Gelatin as culture-media, 231 liquefaction of, 70 punctiu-e culture, 254 Gelatin-media, gelatin, 231 Generation, spontaneous, doctrine of, 17 Gengou-Bordet bacillus, 488 phenomenon, 170 Genital apparatus, infection through, 88 Germ theory of disease, 23 Germicidal value of solutions, method of testing, 304 Germicide, 201 determination of value, 303 Germination of spores, 38 Ghoreyeb's method of staining treponema, 789 Giant-cells in tuberculosis, 731 Gibson's globulin precipitation for concentration of diphtheria serum, 159 Glanders, 775 bacillus of, 775. See also Bacillus mallei. diagnosis, 778 in human beings, 783 specific organism, 775 Glass bench, 248 tubes, capillary, 243, 244 Glassware and instruments, ster- ilization of, 203 protection of, 203 Globulin precipitation for concen- tration of diphtheria serum, 159 Glossina morsitans, 561 palpalis, 561, 563 Glycerin agar-agar as culture- media, 234 Glycerin-gelatin, embedding, 180 Glycoproteids, 134 Golden staphylococcus, 343, 346 Goldhorn's method of staining tre- ponema pallidum, 788 Gonococcus, 431 Gonorrhea, 431 communication of, to animals, 436 diagnosis, 435 Gonotoxin, 435 Gordon's medium for differentia- tion of cholera and Pinkler- Prior spirilla, 615 Gram's method for staining tuber- cle bacillus in sections, 719 of staining, 182, 183, 184 Nicolle's modification, 185 tubercle bacillus in sections, 719 Gram-Weigert method of staining, 185 Granulations of Schiiffner, 537 Granules, Babes-Ernst, 34 metachromatic, 34 polar, 34 Granuloma, coccidioidal, 819 Grass bacillus, Moeller's, 758 Haffkinb's prophylactic, 596, 617 Halogens and compounds as germi- cides, 214 Hands, disinfection of, 209 Hanging block, directions for pre- paring, 174 drop, 173 Hankin and Leumann's method for differential diagnosis of plague bacillus, 588 Haptophore group, 131 Hardening, 178 Healed tubercles, 736 Heat, influence on growth of bac- teria, 64 Heidenhain-Biondi method of stain- ing protozoa in tissue, 199 Heidenhain's method of staining protozoa in tissue, 199 Heiman's method of cultivation of micrococcus gonorrhceae, 434 Helcosoma tropicum, 572, 573 Hematozoa, 528 Hemolysins, 163 Hemolysis, 163 Hemolytic amboceptor for Wasser- mann reaction, 323 serum for Wassermann reaction, titration of, 324 system in Wassermann reaction, 325 Hemorrhagic septicemia, bacilli of, 597, 601 Hemorrhagin, 162 Herpes circinatus, 824 864 Index Herpes desquamans, 824 tonsurans, 824 Hesse's apparatus for collecting bacteria from air, 284 cultiu-e-medium for typhoid ba- cillus, 658 method for quantitative estima- tion of bacteria in air, 284 of making anaerobic culttu-es, 265 Higher bacteria, 41 Hill's hanging block, 174 Hiss' inulin-serum-water test for determining pneumococcus, 505 method of cultivation of typhoid bacillus, 654 of staining diplococcus pneu- monia, 494, 495 Hiss-Russell variety of dysentery bacillus, 702 Histoplasma capsulatiun, 573 Histoplasmosis, 573 Historical introduction, 17 Hoffmann's bacillus, 474. See also Bacillus pseudodiphiksria. Hog-cholera, 678 H6gyes' method of treating hydro- phobia, 419 Host, 78 susceptibility of, loi. See also Susceptibility. Hot-air sterilizar, 203 Huhnercholera, 598 Hydrogen peroxid as germicide, 216 Hydrophobia, 412 communication to man, 412 fixed virus in, 414 scheme for intensive treatment, 418 for mild treatment, 418 street virus in, 414 treatment, 414 Cabot's method, 419 dilution method, 419 Hogyes' method, 419 Pasteur's method, 114, 413, 414,417 Hypnococcus, 557 IcB, bacteria in, 289 Ice-cream poisoning, 299 Ichthyotoxismus, 299 Immune body, 120, 141, 142, 143, I44» 145 Immunity, 105 acquired, 108 accidentally, 109 I Immunity, acquired, active, 109 experimentally, 109 passive, 115 through accidental infection, 109 through bacterination, 113 through infection, 109 through inoculation, 109 through intoxication, 115 through vaccination, no active, 106 acquired, 109 Ehrlich's lateral-chain theory, exhaustion theory, 125 explanation of, 125 Metschnikoff's theory, 126 natural, 107 passive, 106 acquired, 116 relative, 106 retention theory, 125 special phenomena of, 146 synopsis of experimental studies of, 118 to vaccination, 112 variations in, 112 Incubating oven, 258 Incubator for opsonic work, 315 Index, opsonic, 307, 316 India ink method of identifying treponema pallidum, 792 Indol, 73 Salkowski's test for, 73 Infantile kala-azar, 571 Infection, 78 avenues of, 86, 98 cardinal conditions, 95 endogenous, 80 exogenous, 79 exf>erimental, 109 mixed, 103 proteus, 369 sources of, 79 special phenomena of, 146 sub-, 78 terminal, 355 through digestive apparatus, 86 through genital apparatus, 88 through placenta, 88 through respiratory apparatus, 88 through skin, 86 virulence of, 95. See also Viru- lence. Infectious diseases, 339 study, 21 Inflammation, catarrhal, 439 Influenza, 514 Index 86s Influenza, diagnosis, 519 Infusoria, 53 Infusorial life, 19 Injection, intra-abdominal, 271 intrapleural, 271 intravenous, 270 into rabbit, method of making, 270 subcutaneous, 271 Inoculation, 109 advantages of vaccination over, III animal, 271 subcutaneous, 271 Inorganic disinfectants, 213 Instruments and glassware, steril- ization, 203 disinfection of, 209 protection of, 203 surgical sterilization of, 211 Intermediate body, 120 Intermittent sterilization, 205 Intestine, bacteria in, 83 flagellates in, 709 Intoxication, immunity acquired by, 115 Intra-abdominal injection, 271 Intracellular toxins, 90 Intrapleural injections, 271 Intravenous injections, 270 into rabbit, method of making, 270 Introduction, historical, 17 Invasive power of bacteria, 89, 94 Inulin-serum- water test of Hiss for determining pneumococcus, 505 lodin.terchlorid as germicide, 215 Iron-hematoxylin stain for proto- zoa, 199 Isolation of bacteria, 246 Itch, barber's, 827 Jackson's medium for typhoid bacillus in water, 661 Jactationstetanus, 394 Jars, Novy 's, for anaerobic cultures, 261 Javelle water, 720 Jaw, lumpy, 803, 811 Jennerian vaccination, no Kala-azar, 566 diagnosis, 571 infantile, 571 lesions, 570 transmission, 570 Kashida's method of cultivation of typhoid bacillus, 654 55 Kitasato's filter, 207 mouse-holder, 273 Klatschpraparat, 256 Klebs-Lbffler bacillus, ^51. See also Bacillus diphthertee. Knives, sterilization of, 204 Koch-Ehrlich method of staining tubercle bacillus, 715 Koch's agglutination test for tuber- cle bacillus, 746 apparatus for coagulating and sterilizing blood-serum, 235, 236 law of specificity of bacteria, 23 method of isolation of tubercle bacillus, 721 of making anaerobic cultures, 266 of staining tubercle bacillus, 713 syringe, 269 tuberculin, 739 Koch- Weeks bacillus, 446. See also Bacillus of Koch-Weeks. Kolle's method for diagnosis of plague, 594 Krai's method of cultivation of Achorion schdnleinii, 831 Kreotoxismus, 299 Kuhne's method of staining Bacil- lus mallei, 777 La Fibvrb typhique, 632 Lactic acid fermentation, 68 Laitinen's method of cultivation of micrococcus gonorrhoeae, 434 Lamblia intestinalis, 709 Larynx, bacteria in, 85 Latapie's animal holder, 272, 273 instrument for preparing tissue pulp, 165 Latent tuberculosis, 735 Lateral chain-theory of immunity, Ehrlich's, 131 Law, Koch's, of specificity of bac- teria, 23 Leishman- Donovan body, 566 Leishmania donovani, 566 cultivation, 569 distribution, 570 morphology, 568 transmission, 570 furunculosa, 572 infantum, 571 Lepra anaesthetica, 771, 773 cells, 772 nodosa, 771 Leprolin, 774 866 Index Leprosy, 762 anesthetic, 771, 773 etiology, 763 nodular, 771 sanitation in, 774 serum, 774 therapy in, 774 Leptothrix, 41 Lethargy, African, 554 Leuconostoc, 39 Leukocidin, 347 Leukocytes, washed, in testing op- sonic value of blood, 211 Leumann and Hankin's method for differential diagnosis of plague bacillus, 588 Levaditi's method of staining tre- ponema pallidum, 790 Liborius' tube for anaerobic cul- tures, 261 Life, infusorial, 19 spontaneous generation of, doc- trine, 17 Ligatures, disinfection of, 209 sterilization of, 210 Light, effect of, on tubercle bacillus, 727 influences on growth of bacteria, 61 Liquefaction of gelatin, 70 Listerism, 25 Litmus, mediod of preparing, 239 milk as culture-media, 239 Litmus-lactose-agar-agar, 654 Liver, abscess of, in amebic dysen- tery, 697 Lobar pneumonia, 492 Lockjaw, 385. See also Tetanias. Loffler-Klebs bacillus, 451. See also Bacillus diphthericB. Loffler's alkaline methylene-blue, staining Bacillus diphtherise with, 452 blood-serum mixture as culture- media, 236 method for detecting spirillum cholerae Asiaticae, 615 of cultivation of Bacillus diph- theriae, 454 of differentiating typhoid bacil- lus by means of malachite green, 660 of staining, 182 Bacillus mallei, 776 flagella, 189 Luetin, 798 Lugol's solution, dilute, 183 Lumpy jaw, 803, 811 Lungs, bacteria in, 85 Lupus, 99 Lysin, 120 Lysol, 215 Lyssa, 412. See also Hydrophobia. MacConkby's meditmi for ty- phoid bacillus, 661 method for detecting colon bacil- lus in water, 661 Macfadyen's typhoid serum, 648 Macrocytase, 127, 145 Macrogametocyte, 531 Macrophages, 126 Madura-foot, 812 Maladie du coit, 562 du sommeil, 554 Malaria, 524 ague-ccd^e of, 540 algid cases, 539 and mosquitoes, relation, 526, 541 congestive chills of, 539 enlargement of spleen in, 540 estivo-autumnal parasite of, 537 geographic distribution, 524 history, 524 parasites of, 525, 532 animal inoculation, 539 cultivation, 539 human, 532 inoculation, 539 pathogenesis, 539 paroxysms of, 524 prophylaxis, 540 human beings, 540 mosquitoes, 540 quartan, parasite of, 532 temperature in, 524 tertian, parasite of, 534 Malignant carbuncle, 401 edema, 374 polyadenitis, 581 pustule, 407 Mallein, 781 Mallory's differential stain for en- tamoeba, 698 method of staining, 186 Malta fever, 520 bacteriologic diagnosis, 521 treatment, 522 Mastigophora, 51 Matino's method of staining proto- zoa, 198 Measurement of micro-organisms, 200 Meat, bacteria in, 297 extract, preparation of bouillon from, 229 Index 867 Meat, fresh, preparation of bouillon from, 227 Meat-infusion, 228 Meat-poisoning, 69, 299 Meatus, external auditory, bac- teria in, 82 Medical and stu-gical contributions to history of bacteria, 21 Mediterranean fever, 520 Megastomum intestinalis, 709 Meningitis, cerebrospinal, 423 diplococcus of, 423 Meningococcus, 423 Mercuric chlorid as germicide, 213 Mercury, bichlorid of, as germicide, 212 Merismopedia, 38 Merozoits, 529 Metabolism of bacteria, 66 Metachromatic granules, 34 Metschnikoff 's theory of immunity, 126 Meyer and Bergell's typhoid serum, 649 Meyer's sjrringe, 269 Micrococcus, 38 catarrhalis, 439 cultivation, 440 diplococcus intracellularis and, differentiation, 425 morphology, 439 pathogenesis, 441 staining, 440 gonorrhoeae, 431 cultivation, 433 Heiman's method, 434 Laitinen's method, 434 Wassermann's method, 434 Wertheim's method, 433 Wright's method, 434 Young's method, 433 diagnosis of gonorrhea from, 435 distribution, 431 immunization against, 437 isolation, 433 morphology, 432 pathogenesis, 436 staining, 432 toxic products, 435 vital resistance, 434 melitensis, 520 cultivation, 520 morphology, 520 pathogenesis, 522 staining, 520 thermal death-point, 520 pasteuri, 26 tetragenus, 361 Micrococcus tetragenus, cultiva- tion, 362 isolation, 362 morphology, 361 pathogenesis, 363 staining, 362 Microcytase, 127, 145 Microgametocyte, 531 MicromiUimeter, 36 Micro-organisms, ana€robic, cul- tivation of, 260. See also An- aerobic bacteria, cuUivaiion of. cultivation of, 224 in air, 283 measiu'ement of, 200 methods of observing, 172 of plague group, 597 photographing, 200 specific, 339 structure and classification, 29 Microphages, 126 Microscopic study of culttu'es, 259 Microspira, 40 Microsporon, 46 Migula's classification of bacteria, 32 Miliary tubercle, 733 Milk as culture-media, 239 bacteria in, 296 litmus, as culture-media, 239 peptonization of, 76 Milk-poisoning, 298 Milzbrand, 400 Mixed infections, 103 pneumonias, 513 Mixture, Coley's, 358 Moeller's grass bacillus, 758 method of cultivation of smegma bacillus, 758 of staining spores, 188 Moistiu-e, influence on growth of bacteria, 59 Molds, 46 black, 47 influence of food on growth of, 60 of light on growth of, 61 Monilia caudida, 485 Morax-Axenfeld, bacillus of, 448 cultivation, 449 morphology, 449 pathogenesis, 450 staining, 449 Morphology of bacteria, 38 Morro's method of diagnosis of tuberculosis, 741 Mosquitoes and malaria, relation, 526. 541 and yellow fever, 577 classification, 542 868 Index Mosquitoes, destruction of, in pre- vention of malaria, 540 Motility of bacteria, 35 Mouse-holder, 272, 273 Mouth, bacteria in, 82 Movement, influence on growth of bacteria, 63 of protozoa, 55 Mucor, 47 conoides, 48 corymbifer, 47, 48 mucedo, 46, 47, 48 mycosis, 48 pusillus, 48 ramosus, 47 rhizopodiformis, 47 septatus, 48 Muguet, 484 Muir and Ritchie's method of stain- ing spores, 188 MtiUer's fluid, 259 Musgrave and Clegg's agar-agar for cultivation of amebas, 692, 694 Mussel-poisoning, 299 Mycetoma, 812 melanoid form, 813, 816 ochroid variety, 813 pale variety, 813 Mycoderma vini, 485 Mycophylaxis, 129 Mycosis, mucor, 48 Mycosozins, 129 Mytilotoxismus, 299 Myzorrhynchus psueudopictus, 542 Nagana, 560 Needles, platinum, for transferring bacteria, 243 Negri bodies, 421 Neisser and Wechsberg's phenome- non, 167, 168 Neisser's method of staining Bacil- lus diphtheriae, 453 Nephelometer, 310 Nephrolysins, 121 Nephrotoxans, 121 Nessler's solution, 75 Nichols and Schmitter's method of making anaerobic cultures, 263 NicoUe's modification of Gram's method of staining, 185 Nitrate broth, 74 Nitrates, formation of, 74 reduction of, 74 Nitrobacter, 74 Nitrogen, combination of, 75 Nitrosococcus, 74 Nitroso-indol reaction, 73 Nitrosomonas, 74 Nocard's bacillus, 677 Nodular leprosy, 771 Noguchi's cutaneous reaction in diagnosis of syphilis, 798 method of cultivation of trepo- nema pallidtmi, 793 modification of Wassermann re- action, 335 Non-chromogenic bacteria, 71 Non-malarial remittent fever, 566 Non-pathogenic bacteria, 76 Nose, bacteria in, 85 Novy's jars for anaerobic cultures, 261 method of cultivation of smegma bacillus, 757 Nucleus of bacteria, 34 of protozoa, 55 Nuttall and Welch's method of staining bacillus aerogenes cap- sulatus, 380 OBBRMfiifiR's spirillum, 546 Ocular typhoid reaction of Chante- messe, 650 Odors, production of, 73 Oldia, 44 Oldium albicans, 484 cultivation, 486 fermentation, 486 immunity, 487 metabolic products, 486 morphology, 485 pathogenesis, 486 Onychomycosis, 824 Odcysts, 56, 531 Ookinetes, 56, 531 Ophidiomonas, 40 Ophthalmia neonatorum, 450 Ophthalmo-tuberculin reaction of Calmette, 741 of Wolff-Eisner, 742 Opisthotonos in tetanus, 393 Oppler-Boas bacillus in carcinoma of stomach, 83 Opsonic index, 307, 316 value of blood, bacterial suspen- sion in testing, 308 serum in testing, 311 washed leukocytes in test- ing, 3" Opsonins, 127, 307 Opsonizing pipette, 313 Optional anaerobes, 59 Organic disinfectants, 215 Oriental sore, 572 Index 869 Omithodoros moubata, 547, 551 Oshida's method of obtaining rab- bit's cord for use in hydrophobia, 414 Oven, incubating, 258 Oxygen, atmospheric, absorption of, in anaerobic ctiltures, 263 exclusion of, in an€robic cul- tures, 265 influence on growth of bacteria, 59 reduction of, in ana€robic cul- ttu'es, 264 relation of tubercle bacillus to, 727 Oysters, bacteria in, 298 Paludism, 524 Pappenheim's method of staining tubercle bacillus, 717 Paracolon bacilli, 665 Paraffin embedding, 180 Paralysis after use of diphtheria antitoxin, 472 Parasite, 78 stomatitis, 484 Parasites of mialaria, 525, 532 animal inoculation, 539 cultivation, 539 human, 532 inoculation, 539 pathogenesis, 539 Parasitic bacteria, 79 Paratyphoid bacilli, 665 Pariette's culture fluid, 657 Park's method of cultivating teta- nus bacillus, 388 Paroxysms of malaria, 524 Pasteboard cup for receiving in- fectious sputtun, 220 Pasteur-Chamberland filter, 207 Pasteurian vaccination, 113 Pasteurization, 206 Pasteur's protective inoculation against anthrax, 409 treatment of hydrophobia, 114, 413, 414, 417 Pathogenesis, 89 Pathogenic bacteria, 76, 79 protozoa, classification, 51 Pathogens, 66 Patient, disinfection of, 222 Penicillium, 50 crustaceum, 50 glaucum, 50 minimum, 50 Peptone solution as culture-me- dium, 240 Peptonization of milk, 76 Peroxid of hydrogen as germicide, 216 Pertussis, 488 Pest, 581 Petkowitsch's method of isolation of typhoid bacillus, 656 Petri dish forceps, 249 dishes, 249 method for quantitative estima- tion of bacteria in air, 285 sand filter for air-examination, 285 Petruschky's bacillus, 676 whey as culture-media, 240 Pfeiffer's method of staining, 181 phenomenon, 120, 142, 166 Phagocytes, 126 Phagocytic power of blood, 307 Phagocytosis, theory of, 125 Phagolysis, 126 Phenomenon, Bordet-Gengou, 170 Neisser and Wechsberg's, 167, 168 Pfeiffer's, 120, 142, 166 Theobald-Smith, 122 Phlogosin, 347 Phosphorescence, production of, 73 Photogens, 66 Photographing micro-organisms, 200 Phylaxins, 128 Pied de Madura, 812 Pig typhoid, 678 Pink eye, 446 Piorkowski's method of cultivation of typhoid bacillus, 655 Pipette, opsonizing, 313 Pirquet's method of cutaneous di- agnosis of tuberculo- sis, 740 Lignites' modification, 741 Pitfield's method of staining fla- gella, 191 Smith's modification, 191 Pityriasis versicolor, 824 Placenta as avenue of infection for tubercle bacillus, 727 infection through, 88 Plague, 581 buboes in, 583 diagnosis, 593 fleas and, 592, 593 flies and, 592 group micro-organisms of, 597 history, 581 immunity against, 596 mode of infection, 592 • 870 Index Plague pneumonia, 512 sanitation in. structure, 53 Pseudodiphtheria bacillus, 468, 470, 474. See also Bacillus, pseudo- diphtheria. Pseudodysentery bacillus, 699 Pseudoglanders bacillus, 784 Pseudo-influenza bacillus, 519 Pseudomonas, 40 Pseudotetanus bacillus, 399 Pseudotuberculosis, 760 Psittacosis, 677 Ptomains, 68 definition of, 68 Pulex cheopis, 593 irritans, 593 Pure culture, 242, 252 Index 871 Pure culture, special methods of securing, 256 Pustule, malignant, 407 Putrefaction, 20, 68 Putrefactive ferment, 24 Pyemia, 94 Pyocyanase, 77, 367 Pyocyanin, 366 Pyocyanolysin, 367 Quartan malarial fever, parasite of, 532 Rabbit septicemia, bacillus of, 598, 601 Rabies, 412. See also Hydrophobia. Ravenel's method of preparation of agar-agar, 233 potato cutter, 238 Ray-fungi, 804 Receptors, 132, 153 Refined tuberculin, 739 Regressive schizogony, 532 Reichel and Bugle's stain for Negri bodies, 421 Reichel's filter, 208 Relapsing fever, 546 bacteriologic diagnosis, 553 course, 552 immimity to, 553 lesions of, 553 Remittent fever, non-malarial, 56 Rtoy's method of cultivation of typhoid bacillus, 653 Reproduction of bacteria, 36 of protozoa, 56 Respiratory apparatus, infection through, 88 tract as avenue of infection for tubercle bacillus, 728 Retention theory of immunity, 125 Rhinoscleroma, 785 Rhipicephalus decoloratus, 546 Rhizopoda, 51 Rice-water discharges of Asiatic cholera, 613 Richardson's method of dififerenti- ating typhoid bacillus, 652 Ringworm, 824 of body, 824 of scalp, 824 Ritchie and Muir's method of stain- ing sp>ores, 188 Romanowsky's method of staining protozoa, 197 Ross' method of staining protozoa, 200 Rossi's method of staining flagella, 193 Rost's method of cultivation of lepra bacillus, 766 Rothberger's method of cultivation of typhoid bacillus, 654 Rouget du Pore, 26 Roux's syringe, 269 R tuberculin, 742 Rubber stoppers, sterilization of, 204 Saccharomycbs hominis, 44, 818 Saccharomycosb hominis, 818 Salkowski's test for indol, 73 Salomonsen's method of making ana&x>bic cultures, 266 Salts as germicides, 213 Sanarelli's bacillus, 682 Sand filter, Petri's, for air-examina- tion, 285 Sapremia, 94 Saprogens, 6i5 Saprophytic bacteria, 79 Sardna, 39 Sarcoma, Coley's mixture in, 358 Scalp, ringworm of, 824 Scarlatina, streptococcus in blood in» 355 Schaumorgane, 384 Schizogony, regressive, 532 Schizont, 529 Schlafkrankheit, 554 Schmitter and Nichols' method of making ana&'obic cultures, 263 Schottelius' method of detecting spirillum cholers Asiatics in feces, 609 of securing pi^e cultures of spiriUtun cholerse Asiatics, 609 Schuffner's granulations, 537 Scissors, sterilization of, 204 Scutuliun, 828 Sedgwick's expanded tube for air- examination, 285 method for quantitative estima- tion of bacteria in air, 286 Seitenkettentheorie, 131 Semmelformig, 432 Septicemia, 94 hemorrhagic, bacilli of, 597 rabbit, bacillus of, 598, 601 Serum, anticholera, 617 antigonococcus, 437 antimeningococcus, 430 antipneumococcus, 505, 506 antirabic, 422 872 Index Serum, antistaphylococcus, 349 antistreptococctis, 359 antitubercle, 746 antityphoid, 648 antivenomous, 161 diagnosis of syphilis, 797 disease, 123 hemolytic, for Wassermann re- action, titration of, 324 immune, against pneumococcus, 505 in testing opsonic value of blood, 311 leprosy, 774 plague, 596, 597 tetanus antitoxic, 160 therapy in anthrax, 410 in Asiatic cholera, 617 in bacillary dysentery, 704 to be tested in Wassermann re- action, 320 Yersin's, 597 Sexual apparatus as avenue of in- fection for tuberde bacillus, 729 Shake culture, 265 Sheep corpuscles, titration of, for Wassermann reaction, 324 Shell-fish, bacteria in, 298 Shiga-Kruse variety of dysentery bacillus, 702 Shiga's bacillus, 699. Siberian pest, 400 Sick-chambers, disinfection of, 211 Sickness, sleeping-, 554. See also Sleeping-sickness. Sick-room, air of, disinfection, 211 Silver nitrate as germicide, 214 Size of bacteria, 36 of protozoa, 56 Skin and adjacent mucous mem- branes, bacteria in, 80 infection through, 86 Sleeping-sickness, 554 prophylaxis, 563 specific organism, 555 transmission, 559 to lower animals, 562 Smegma bacillus, 757. See also Bacillus smegmatis. Smith's fermentation-tube, 69 method for determining bacillus coli communis in water, 291 nature of gases, 70 for isolation of tubercle bacil- lus, 723 modification of Newman's meth- od of staining flagella, 194 Smith's modification of Pitfield's method of staining flagella, 191 phenomenon, 122 tube for isolation of tubercle bacillus, 723 Soil, bacteria in, 294 Prankel's method of estimat- ing number, 294 bacteriology of, 294 Soluble toxins, 89, 91 Solvent, 120 Somers' modification of Starkey's labyrinth, 658 Soor, 484 Sozins, 128 Spasm in tetanus, 393 Specific micro-organisms, 339 Spermatolysis, 164 Spermatoxin, 121 Spermotoxin, 121 anti-, 121 Spinal cord of rabbit, attenuation, for use in hydrophobia, 416 method of obtainmg, for use in hydrophobia, 414 Spirilla resembling cholera spiril- lum, 619 table for differentiating, 631 Spirillum, 38, 40 cholerx Asiatics, cultivation, 609 detection, 615 Ldffler's method, 615 distribution, 605 general characteristics, 604 immunity against, 616 in feces, Schottelius' method of detecting, 609 isolation, 608 Ldffler's method of detect- ing, 615 metabolic products, 612 morphology, 606 pathogenesis, 613 Schottelius' method of mak- ing pure cultures of, 609 serum therapy of, 617 specificity, 615 spirilla resembling, 619 table for differentiating, 631 staining, 608 Comil and Babes' method, 608 toxic products, 612 vital resistance, 611 of Denecke, 622 Index 873 spirillum of Denecke, cultivation, 623 metabolic products, 624 morphology, 622 pathogenesis, 624 of Finkler and Prior, 619 cultivation, 619 metabolic products, 622 morphology, 619 pathogenesis, 622 staining, 619 of Gamal^ia, 625 ctdtivation, 625 immunity against, 628 metabolic products, 627 morphology, 625 pathogenesis, 627 staining, 625 vital resistance, 627 of Obermeier, 546 Spirochseta, 40 anserinum, 546 carteri, 548 dentinum, 546 duttoni, 547, 548 gallinarum, 546 novyi, 548 obermeieri, 546, 547, 548 cultivation, 550 general characteristics, 548 mode of infection, 550 morphology, 548 pathogenesis, 552 staining, 550 pallida, 787 . See also. Treponema pallidum. pallidula, 801 pertenuis, 801 refringens, 787, 799 theileri, 546 vincenti, 479 and BacUlus fusiformis, re- lation, 479 cultivation, 480 morphology, 480 pathogenesis, 483 Spiromonas, 40 Spirosoma, 40 Spirulina, 40 Spleen, enlargement of, in malaria, 540 Splenic fever, 400 Splenomegaly, febrile tropical, 566 Spontaneous generation, doctrine of, 17 Sfjores, 36 germination of, 38 method of staining, 187. See also Staining spores. Spores of Plasmodium malaris, 529 Sporocysts, 56 Sporozoa, 52 funmculosa, 573 Sporozoits, 528, 531 Sporulation, 36 Spotted fever, 423 Sputum, Bacillus typhosus in, 644 infectious, pasteboard cup for receiving, 220 tubercle bacillus in, staining, 714 Stain, eosin and methylene-blue, 186 iron-hematoxylin, for protozoa, 199 Staining, 174 aqueous solution, 177 flagella, Ldffler's method, 189 method of, 189 Pitfield's method, 191 Smith's modification, 191 Rossi's method, 193 Smith's modification of New- man's method, 194 Van Ermengem's method, 192 Gram's method, 182, 183, 184 Nicolle's modification, 185 Gram-Weigert method, 185 jar, Coplin's, 181 Ldffler's method, 182 Mallory's method, 186 Pfeiffer's method, 181 preparations for general examin- ation, 975 protozoa, 195 Biondi-Heidenhain method, 199 cover-glasses, 196 Heidenhain's method, 199 in tissue, 199 Marino's method, 198 Romanowsky's method, 197 Ross' method, 200 slides, 196 Wright's method, 197 simple method, 176, 181 spores, Abbott's method, 188 Anjeszky's method, 188 Piocca's method, 189 method of, 187 Mdller's method, 188 Muir and Ritchie's method, 188 stock solutions, 177 Zieler's method, 186 Standard reaction of culture-media, 227 Standardizing freshly isolated cul- tures, 259 874 Index Staphylococci, chief types, table of, 342 Staphylococcus, 39 citreus, 350 epidermiditis albus, 341 golden, 343, 346 pyogenes albus, 343 aureus, 343 et albus, 344 agglutination, 349 bacterio-vacdnation, 349 cultivation, 345 distribution, 344 isolation, 344 morphology, 344 pathogenesis, 347 staining, 344 thermal death-point, 346 toxic products, 346 treatment with serum, 349 virulence, 349 Staphylolysin, 347 Starkey's labyrinth modified by Somers, 658 method of isolation of typhoid bacillus, 657 Steam sterilizer, Arnold's, 205 Stegomyia calopus, 578 fasciata, 578 seu calopus, 577 Stemphylium polymorpha, 485 Sterilization and disinfection, 201 and protection of culture-media, 205 by filtration, 208 in autoclave, 206 intermittent, 205 of catgut, 210 Cladius' method, 211 cumol method, 211 of instruments and glassware, 203 of ligatures, 210 of platinum wires, 203 of surgical instruments, 211 Sterilizer, hot-air, 203 Stewart's cover-glass forceps, 177 Stock solutions, 177 Stomach, bacteria in, 83 carcinoma of, Oppler-Boas bacil- lus in, 83 Stomatitis, parasite, 484 Street virus in hydrophobia, 414 Streptobacillus, 443 Streptococcus, 39 brevis, 351 conglomeratus, 352 diffusus, 352 erysipelatis, 361 in blood in scarlatina, 355 Streptococcus longus, 361 mucosus, 359 pyogenes, 350 cultivation, 351 differential features, 353 isolation, 351 morphology, 351 pathogenesis, 354 relation to diphtheria, 354 staining, 351 toxic products, 357 virulence, 356 vital resistance, 353 viridans, 354 Streptokolysin, 357 Streptothrix, 42 enteola, 42 farcinica, 43 Structtue of bacteria, 34 of protozoa, 53 Subcutaneous injection, 271 inoculation, 271 Subinfection, 78 Substance sensibiiisatrice, 144, 145 Sucholotoxin, 681 Sugar bouillon, 230 Suppuration, 339 amebse and, 372 amoeba kartidisi as cause, 372 mortinatalium as cause, 373 bacteria associated with, 341 entamoeba buccalis as cause, 372 miscellaneous organisms of, 373 Surgical contributions to history of bacteria, 21 instruments, sterilization of, 211 Susceptibility, loi diet as cause, 102 exposure to cold as cause, 102 fatigue as cause, loi inhalation of noxious vapors as cause, 10 1 intoxication as cause, 102 morbid conditions in general as cause, 103 traumatic injury as cause, 103 Susotoxin, 680 Sutiu-es, disinfection of, 209 Swine-plague, bacillus of, 601 Symbiosis, influence on growth of bacteria, 63 Synopsis, proposed, of groups of bacteria, 279 Syphilis, 787 bacillus of, 787 diagnosis, 797 lesions of, 797 Noguchi's cutaneous reaction in diagnosis of, 798 Index 875 Syphilis, serum diagnosis, 797 Wassermann reaction for diag- nosis of, 318. Syphilitic antigen, 319 titration of, 328 Syringe, Altmann's, 269 bacteriologic, 269 Koch's, 269 Meyer's, 269 Roux's, 269 Tbmpbraturb in malaria, 524 influence on growth of bacteria. Terminal infections, 355 Tertian malarial fever, parasite of, 534 Tetanin, 391 Tetanolysin, 91, 120, 131, 391, 394 Tetanospasmin, 91, 391 Tetanotoxin, 391 Tetanus, 385 after use of diphtheria antitoxin, 473 antitoxic serum, 160 antitoxin, 160, 397 ascendens, 393 bacillus, 385. See also Bacillus tetani. clonic convulsions in, 393 descendens, 393 opisthotonos in, 393 pathogenesis, 394 prophylactic treatment, 398 spasm in, 393 tonic convulsions in, 393 Tetracoccus, 38 Theobald-Smith phenomenon, 122 Theory, Ehrlich's lateral-chain, of immtmity, 131 Metschnikofif's, of immunity, 126 Thermal death-point of bacteria, determination, 300 Thermophilic bacteria, 64 Thrush, 484 Tinea circinata, 824 favosa, 828 imbricata, 824 trichophytina, 824 unguiimi, 824 versicolor, 824 Tongue, wooden, 803, 811 Tonic convulsions in tetanus, 393 Torrey's antigonococcus serum, 437 Toxemia, 94 Toxic power of bacteria, 89 Toxins, extracellular, 89 intracellular, 89, 91 soluble, 89, 91 specific action, 92 affinity of cells for, 93. Toxophile groups, 133 Toxophore group, 131 Toxophylaxins, 129 Toxosozins, 129 T R tuberculin, 742 Trachea, bacteria in, 85 Treponema, 40 pallidum, 787 Burri's India ink method of identifjdng, 792 cultivation, 793 Noguchi's method, 793 distribution, 792 general characteristics, 787 morphology, 788 pathogenesis and specificity. 795 staining, 788 Ghoreyeb's method, 789 Goldhom's method, 788 Levaditi's method, 790 pertenue, 800 cultivation, 802 morphology, 801 pathogenesis, 802 staining, 801 Trichomonas intestinalis, 709 Trichophyton, 46 acuminatum, 825 circonvulatimi, S25 crateriforme, 825 effractum, 825 exsiccatum, 825 flavum, 825 fulminatum, 825 glabrum, 825 megalosporon, 825 microsporon, 825 pilosum, 825 plicatili, 825 polygonum, 825 regulare, 825 sulphureum, 825 tonsurans, 824 cultivation, 825 morphology, 825 pathogenesis, 826 umbilicatum, 825 violaceum, 825 Trikresol, 215 Trommelschlager, 37 Tropical splenomegaly, febrile, 566 ulcer, 572 Trypanosoma avium, 556 876 Index Tnrpanosoma brucei, 556 castellani, 557 cruzi, 56^ transmission, 565 damonise, 556 equinum, 556 equiperdum, 562 gambiense, 554 cultivation, 558 morphology, 558 pathogenesis, 562 reproduction, 559 staining, 558 transmission, 559 to lower animab, 562 lewisi, 556 rajae, 556 rotatorium, 556 theileri, 556 transvaliense, 556 ugandense, 557 various species, 556 Trypanosomiasis, American, 564 human, 554 transmission, 565 Tryptic enzymes, 71 Tse-tse-fly disease, 560 Tubercle bacillus, 710. See also Bacillus tuberculosis. Tubercles, 733 crude, 733 healed, 736 miliary, 733 of Babes, 419 Tubercular abscess, 731 pneumonia, 512 Tuberculin, 738 concentrated, 739 crude, 738 Denys', 742 diluted, 729 influence on tuberculous tissue, 740 Koch's, 739 preparation of, 738 refined, 739 test for tuberculosis of cattle, 754 Tuberculin-R, 742 Tuberculin-T R, 742 Tuberculinic acid, 737 Tuberculocidin, 742, 743 Tuberculosamin, 737 Tuberculosis, 710 bacillus of, 710. See also Bacillus tuberculosis. bovine, 748 communicability to man, 750 prophylaxis, 753 tuberculin test for, 754 Tuberculosis, diagnosis, Calmette*s ophthalmo-tuberculin reac- tion, 741 Morro's method, 741 von Pirquet's cutaneous meth- od, 740 Ligni^rcs' modification, 741 Wolff-Eisner ophthalmo-tu- berculin method, 742 fish, 756 fowl, 754 giant-cells in, 731 latent, 735 lesions of, 730 of cattle, tuberculin test fen*, 754 prophylaxis, 747 pseudo-, 760 specific organism, 711 Tuberculous abscess, 731 Tubes, capillary glass, 243, 244 Esmarch's, 250 method of holding, during inocu- lation, 2^5 Typhoid earners, 640 fever, 632 bacillus of, 632 bacteriologic diagnosis, 649 blood-culture in, 650 carriers, 640 conjunctival reaction in, 650 histologic lesions, 642 in lower animals, 644 isolation of bacillus from feces in, 650 pathogenesis, 639 prophylactic vaccination against, 646 prophylaxis, 645 specific therapy, 647 Widal reaction in, 649 pig, 678 reaction of Chantemesse, 650 Typhus abdominalis, 632 Tyrotoxicon, 68, 299 Tyrotoxismus, 299 Ulcbr, tropical, 572 Umstimmung, 798 Unna's method for staining tuber- cle bacillus in sections, 719 Urethra, bacteria in, 85 Urine, Bacillus tuberculosis in, staining, 718 Bacillus typhosus in, 643 smegma bacillus in, 718 Uterus, bacteria in, 85 Index 877 Vaccination, iio accidents of, 112 advantages of, over inoculation, III bacterio-, in staphylococcic in- fections, 349 efficient, 112 immunity to, 112 in anthrax, 409 inefficient, 112 Jennerian, no Pasteurian, 113 prophylactic, against typhoid fever, 646 Vaccine, nature of, in Vaccines, 113 Vaccinia, 112 Vacuoles, contractile, 54 Vacuum, formation of, in anaerobic cultures, 260 Vagina, bacteria in, 85 Van Ermengem's method of stain- ing flagella, 192 Vibrio, 40 lineola, 799 Metschnikovi, 625 proteus, 619 schuylkiliensis, 628 cultivation, 628 immunity against, 629 metabolic products, 628 morphology, 628 pathogenesis, 629 vital resistance, 629 tyrogenum, 622 Vibrion septique, 374 Vibrionensepticsemia, 628 Vincent's angina, 478 Virulence, 95 decrease of, 95 increase of, 96 by addition of animal fluids to culture-media, 97 by i>assage through animals, 96 by use of collodion sacs, 96 number of bacteria influencing, 97 Virus, fixed, in hydrophobia, 414 street, in hydrophobia, 414 von Pirquet's method of cutaneous diagnosis of tubercu- losis,, 740 Ligni6res' modification, ! 741 Washed leukocytes in testing op- sonic value of blood, 311 Wassermann method of cultivation of micrococcus gonorrhces, 434 Wassermann reaction, 318 amboceptor dose in, 326 unit in, 325, 326 antigen in, 319 titration of, 328 blood-corpuscles for, 322 titration of, 324 complement for, 321 titration of, 324 hemolytic amboceptor for, 323 serum for, titration of, 324 system in, 325 nature of, 335 Noguchi's modification, 335 reagents employed, 318 serum to be tested, 320 validity of, 334 Water, Bacillus coli communis in, 291 bacteria in, 287 method of determining num- ber, 287 Winslow and Willcomb's di- rect method of enumeration of, 288 bacteriology of, 287 colon bacillus in, 674 MacConkey's medium for detecting, 661 Wurtz's medium for detect- ing, 675 typhoid bacillus in, Starkey's method of isolating, 657 Wechsberg and Neisser's phenome- non, 167, 168 Weeks-Koch bacillus, 446. Weigert-Gram method of staining, 185 Welch and Nuttall's method of staining bacillus aerogenes cap- sulatus, 379 Welch's method of staining Dip- lococcus pneumoniae, 494 Wertheim's method of cultivation of micrococcus gonorrhoeae, 433 Wesbrook's types of Bacillus dip- theriae, 452, 469 Whey, Petruschky's, as culture- medium, 240 Whooping-cough, 488 Widal reaction, 149, 649 Willcomb and Winslow's direct method of enumeration of bac- teria in water, 288 Williams' method for cultivation of ameba, 695 878 Index Winslow and Prescott's method of preparing litmus, 239 and Willcomb's direct method of enumeration of bacteria in water, 288 Wolff-Eisner ophthalmo-tubercu- lin reaction, 742 Wolfhfigel's apparatus for counting colonies of bacteria upon plates, 287 Wooden apparatus, sterilization of, 204 tongue, 803, 811 Wounds as avenue of infection for tubercle bacillus, 730 disinfection of, 211 Wright's method of cultivation of micrococcus gonorrhces, 434 of enumerating bacteria in fluid, 289 of making anaerobic cultures, 264, 267 of staining protozoa, 197 Wiirtz's method for. detecting colon bacillus in water, 675 X-RAYS, influence on growth of bacteria, 62, 63 Yaws, 800 Y bacillus, 700 Yeasts, 43, 818 influence of food on growth of, 60 of light on growth of, 61 Yellow fever, 576 mosquitoes and, 577 prophylaxis, 580 Yersin's serum, 597 Young's method of cultivation of micrococcus gonorrhoeae, 433 Zbnkbr's fluid, 179 Ziehl's method of staining Bacillus typhosus, 634 tubercle bacillus, 716 Zieler's method of staining, 186 Zinsser's method of making anae- robic cultures, 264 Zopf 's bacterium pneumoniae, 507 Zur Nedden's bacillus, 450 Zygote, 531 SAUNDERS' BOOKS on • Pathology, Physiology Histology, Embryology and Bacteriology W. B. SAUNDERS COMPANY 925 WALNUT STR££T PHILADELPHIA 9. HENRIETTA STREET COVENT GARDEN, LONDON LITERARY SUPERIORITY ^■^HE excellent judgment displayed in the publications of the house * at the very beginning of its career, and the success of the mod- ern business methods employed by it, at once attracted the attention of leading men in the profession, and many of the most prominent writers of America offered their books for publication. Thus, there were produced in rapid succession a number of works that imme- diately placed the house in the front rank of Medical Publishers. One need only cite such instances as Musser and Kelly's Treatment, Keen's Surgery, Kelly and Noble's Gynecology and Abdominal Sur- gery, Cabot's Differential Diagnosis, Mumford's Surgery, Cotton's Dis- locations and Joint Fractures, Crandon's Surgical After-treatment, Sisson's Veterinary Anatomy, Anders and Boston's Medical Diagnosis, Bonney's Tuberculosis, Gant's Constipation and Obstruction, Jordan's Bacteriology, and Kemp on Stomach and Intestines. These book3 have made for themselves a place among the best works on their respective subjects. A Complete Catalogue of our Publicatioiit will be Sent upon Request SAUNDERS' BOOKS ON Jordan's General Bacteriology A Text-Book of Qeneral Bacteriology. By Edwin O. Jordan, Ph.D., Professor of Bacteriology in the University of Chicago and in Rush Medical College. Octavo of 594 pages, illustrated. Cloth, ^3.00 net. THE NEW (2d) EDmON Professor Jordan's work embraces the entire field of bacteriology, the non- pathogenic as well as the pathogenic bacteria being considered, giving greater emphasis, of course, to the latter. There are extensive chapters on methods of studying bacteria, including staining, biochemical tests, cultures, etc.; on the development and composition of bacteria ; on enzymes and fermentation-products; on the bacterial production of pigment, acid and alkali ; and on ptomains and toxins. Especially complete is the presentation of the serum treatment of gonor- rhea, diphtheria, dysentery, and tetanus. The relation of bovine to human tuberculosis and the ocular tuberculin reaction receive extensive consideration. This work will also appeal to academic and scientific students. It contains chapters on the bacteriology of plants, milk and milk-products, air, agriculture, water, food preservatives, the processes of leather tanning, tobacco curing, and vinegar making ; the relation of bacteriology to household administration and to sanitary engineering, etc. Prof* Sevennce Burra^e* Associate Professor of Sanitary Science, Purdue University. 