https://www.cea.fr:443/cea-tech/pns/nanosafe/en/Documents/2020-Nanosafe/NS20-Posters/NS20_Poster_1_1044_Radiolabeling-As-a-Versatile-Tool-in-nanosafety-Research.pdf
Titel Vorname Name | Institut | Abteilung | E-Mail | www.hzdr.de
Radiolabeling as a versatile tool in nanosafety
research – Accurate quantification in complex
Application: (Nano-)Particle Tracing
Interaction with soil bacteria:
Irradiation time [h]
Release of [48V]TiO2 NPs from UV-degraded
surface coatings
Release from solids: Transport in liquids:
ζ-Potential [mV]
NP - 41.8 - 12.3 + 41.3
Matrix - 50.3 - 12.6 - 35.0
Transport of [48V]TiO2 NPs
Visualization and quantification
of complex processes in
complex systems with very low
FS3 FS3/10 mM
Radiolabeling methods
p+ ,n
Radiosynthesis Radiochemistry Recoil-labeling Activation In-diffusion
e.g.: [110mAg]Ag NPs e.g.: [125I]CNTs e.g.: [7Be]MWCNTs e.g.: [48V]TiO2 NPs e.g.: [139Ce]CeO2 NPs
stable nuclide radioactive nuclide
: 7Li compound : 7Be
: MWCNT
Method Particle Halflife Activity Detection limit
[125I]fulvic acid
53.0 d
Cyclotron techniques
Many studies in the field of nanosafety research „do not offer any kind of clear statement on the safety of nanomaterials“[1], as
methodological problems considerably hinder the reliable detection of nanoparticles (NPs)[2] at the predicted low
environmentally relevant concentrations[3].
The radiolabeling of nanoparticles has the potential for detecting nanoparticles at minimal concentrations in complex matrices,
even against a same-element background, with unprecedented experimental ease, making it a versatile tool for NP release,
transport, and uptake studies.
COSTIS target station
Established nuclide
production routines:
11C, 18F, 45Ti, 64Cu,
135La, 89Zr, 48V, 51Cr,
85Sr, 56/58Co, 86/88Y,
Target capsules for NP irradiation
[4] [5] [6] [7] [8]
[1] Krug, Angew. Chem. Int. Ed. 53, 2014. [2] Von der Kammer et al., Environ.Toxicol. Chem. 31(1), 2012. [3] Gottschalk et al., Environ.Pollut. 181, 2013. [4] Ichedef et al., J. Nanopart.Res. 15(11), 2013. [5] Franke et al., ES&T 42(11), 2008.
[6] Holzwarth et al., J. Nanopart.Res. 16(9), 2014. [7] 2014 Hildebrand et al., J. Nanopart.Res. 17(278), 2015. [8] Hildebrand & Franke, J. Nanopart.Res. 14(10), 2012. [9] Schymura et al., Angew. Chem. Int. Ed. 56, 2017..
Uptake by plants:[9]
no degradation
visible with SEM NP release detectable by radiolabeling
localization of NP release
retention rates of 100 ng TiO2 on
7x1 cm glass bead (200 µm) column
deposition pattern of TiO2
along the column
Particulate uptake of [139Ce]CeO2 by sunflower
target window
sets proton
start 10 days
NP irradiation
IBA Cyclone 18/9
[139Ce]CeO2 NPs accumulate
in leaf veins and edges
aq. [139Ce]CeCl3 shows
equal distribution
Stefan Schymura1, Heike Hildebrand1, Iarsolav Rybkin1,2, Thomas Fricke3, Alexander Mansel1, Marcel Neugebauer4, Anette
Freyer5, Tomaz Rijavec2, Ales Lapanje2, Marko Strok2, Thomas Lange5, Uwe Holzwarth6, Neil Gibson6, Karsten Franke1
1 HZDR, Institute of Resource Ecology, Leipzig, Germany 2 Jozef Stefan Institute, Environmental Sciences, Ljubljana, Slovenia
3 Vita34 AG, BioPlanta, Leipzig, Germany 4AUD, Chemnitz, Germany 5IOM, Leipzig, Germany 6 European Commission, Joint Research Centre, Ispra, Italy
Fate in waste water treatment:
Dissolution of [75Se]CdSe/[65Zn]ZnS NPs
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22
Operation time [d]
sludge water
Dissolution of [141/139Ce]CeO2
development of Zn/Se ratio in effluents
acids 0
t [d]
dissolution of CeO2 at pH 3