Python program to Website Application
I have been told that pylons is quite good (newer pyramid), but I personally use Django and I'm quite happy with it.  Do not even try to use CGI because that's the same mistake I made - and I figured out later that changing all the html was a pain in the arse.
I'm working on a project which is converting a 50mb python Graduated interval recall rating system for pictures and text program to a website based application. (and then design a website around it) It needs to connect to a database to store user information frequently so it needs to be run server side correct? Assuming I know nothing, what is the best structure to complete this?  There seem like many different options and I feel lost.
I've been using CGI to create a web UI for the original python code.  Is this even possible to implement?
What about
pyramid / uWSGI / pylon / flask
or Django? (although I was told to refrain from it for this project)