Does print statement will make performance issue for a website
Yes, since the arguments will need to be converted to strings before outputting and that can be a costly operation.
However, using print causes another issue. Some WSGI containers, notably mod_wsgi among them, do not like anything sent to stdout by default and so will raise an exception whenever that happens. You can fix this by printing to stderr instead, but the performance issue will remain.
We are using the python/Django for web development. While development phase wile coding i tends to put print statement a lot places to check the control flow of the code.
And same code with print statement is uploaded for server,
I know logging is a place to be more suitable but i find myself more comfortable with print.
I want to know how few lines of code will make site slow, if it makes it slow.
Lets say i have 100 line of code and upon that i added 5 print statements. another way of looking into it is to i wrote a 105 line of code. Then in that sense i should not make difference (i suppose). 
Does this will make the website slow ?