Optimization Techniques in Python
ok, not entirely to the point, but before you go and start fixing it, make sure everyone understands the situation. it seems to me that they're putting some pressure on you to fix the "problem".
well first of all, when you wrote the application, have they specified the performance requirements? did they tell you that they need operation X to take less than Y secs to complete? Did they specify how many concurrent users must be supported without penalty to the performance? If not, then tell them to back off and that it is iteration (phase, stage, whatever) one of the deployment, and the main goal was the functionality and testing. phase two is performance improvements. let them (with your help obviously) come up with some non functional requirements for the performance of your system.
by doing all this, a) you'll remove the pressure applied by the finance team (and i know they can be a real pain in the bum) b) both you and your clients will have a clear idea of what you mean by "performance" c) you'll have a base that you can measure your progress and most importantly d) you'll have some agreed time to implement/fix the performance issues.
PS. that aside, look at the indexing... :)
Recently i have developed a billing application for my company with Python/Django. For few months everything was fine but now i am observing that the performance is dropping because of more and more users using that applications. Now the problem is that the application is now very critical for the finance team. Now the finance team are after my life for sorting out the performance issue. I have no other option but to find a way to increase the performance of the billing application. 
So do you guys know any performance optimization techniques in python that will really help me   with the scalability issue
Guys we are using mysql database and its hosted on apache web server on Linux box. Secondly what i have noticed more is the over all application is slow and not the database transactional part. For example once the application is loaded then it works fine but if they navigate to other link on that application then it takes a whole lot of time.
And yes we are using HTML, CSS and Javascript