I have Django app that presents a list of items that you can add comments to. 
What i basically want to do is something like the Facebook did: when someone post a comment on your item, you will receive an e-mail. What I want to do, is when you reply to that e-mail, the reply to be posted as a comment reply on the website. 
What should I use to achieve this using python as much as possible ? Maybe even Django ?
You can for example write script for importing comments from mailbox(for example 1-3 minutes for cron).
You should connect to special mailbox which collects replies from users(comments).
Every mail have own header and title. You really can find out which post user try to comment(by header or title), and then import django enviroment and insert new recods.
How to post a comment on e-mail reply?