I work mostly with Matlab but I am little bit familiar with Python as well. I have never created a GUI before, however, I have been suggested to develop a GUI for an important project in Python and not in Matlab. 
Considering this situation, are there any suggestions that you would like to give which could help me create GUI in python in a time period of a month? Also, I would like to know if I can use Python's Spyder IDE and create GUI from that platform?
I would definitely vouch for wxpython. It's very good for advanced GUI work.
Creating Graphical User Interface (GUI) in Python