I wrote a program in Python, then created a GUI using Tkinter. When I use programs on my computer (like Microsoft Word), I don't need to access the GUI from the command line I just click the application icon.
How do I put my program (the program itself is in the same .py file as the GUI) into an application icon that will start my program?
If you're using Windows, you can create a shortcut. Or you can just put your .py file on your desktop.
If you want to make an .exe file I would suggest installing py2exe. I've compiled Tkinter Python scripts into .exes with py2exe many  times.
how to create an icon for my python application