I'm really trying to fall in love with Python and move away from Matlab.  Is it possible to make Matlab style GUIs in Python?  How easy is it by comparison?  (I make matlab GUIs programmatically, wouldn't dare use GUIDE)  Can I put matplotlib graphics in these GUIs?  Is tk or wx (or something else) better for this?
Haven't used Matlab before, not sure about it's GUI. But if you tend to use Python interactively, you may want to give iPython a try. Ipython with Qt can render you an elegant GUI.
Can python make Matlab style GUIs