I'm designing a program in python tkinter that displays information that's currently in an excel spreadsheet. Ideally, I'd like to be able to share this program without needing to share the excel book as well. Is it possible to import the excel book into the program to make the program independent of that excel file? Let me know if I can provide any more clarification. Thank you!
You would need to assign the Excel file's content to a variable in your program to do so. I hope it isn't very large, but to make it easier I recommend:

saving your Excel file to .csv format
read it from Pytho
convert it to the data type you want (ex: string, list, ...)
save it back to a .txt file.

Now just copy the content of your .txt and assign that to a variable somewhere in your code.
Edit: what Alphy13 said is also a solution
How do I make my program independent of an excel sheet it currently relies on?