I'm trying to import a load data from an Excel file to Abaqus Amplitude object by using python script, so that I can automate the preprocess to realize a large number of load conditions. But there is an Error: "ValueError: File 'load.xlsx' is already in use. Close the file before importing the data."
However, I have never opened this Excel file. I have reboot the computer to make sure the file is not opened. This error still appears. Below is what I entered in the Kernel Command Line Interface in Abaqus and the response: 



import abq_ExcelUtilities
abq_ExcelUtilities.excelUtilities.ExcelToAmplitude(inputFileForAmp='load.xlsx',sheetNameForAmp='Sheet1', ampStartCell='A1', ampEndCell='B34972', ampNameStr='Amp-1', amplitudeType=0)
      Importing file "load.xlsx"...
      File "SMAPyaModules\SMAPyaPluginsPy.m\src\abaqus_plugins\excelUtilities\abq_ExcelUtilities\excelUtilities.py", line 465, in ExcelToAmplitude
      File "SMAPyaModules\SMAPyaPluginsPy.m\src\abaqus_plugins\excelUtilities\abq_ExcelUtilities\excelUtilities.py", line 682, in CreateObject
      File "SMAPyaModules\SMAPyaPluginsPy.m\src\abaqus_plugins\excelUtilities\abq_ExcelUtilities\excelUtilities.py", line 512, in ExtractDataFromExcel
      ValueError: File 'load.xlsx'
      is already in use. Close the file before importing the data.



I have no idea where to begin to address this problem. Any help would be greatly appreciated.
I solved this problem by close all software in the Windows tray. One of the softwares that startup along with Windows may obstructs my script. But when I reboot my PC again to trial out which one caused this trouble, the problem totally disappeared, and never show up. 
Thanks to @Tom for his concern! Any good insight is welcomed.
How to solve "ValueError: File 'load.xlsx' is already in use." error encountered when use 'abq_ExcelUtilities.excelUtilities.ExcelToAmplitude' method?