The following strategies to overcome diffusion limitation in growing cells in suspension are most feasible with a low budget:

Increase the agitation rate: Agitation can help to mix the nutrients, oxygen, and waste products in the culture media and improve the rate of diffusion. By increasing the agitation rate to 270 rpm, you can help overcome the diffusion limitation in your cell culture.

Optimize the culture conditions: Optimizing the culture conditions such as temperature, pH, and oxygen levels can help to improve the rate of diffusion and cell growth. By maintaining a temperature of 37°C, you are already taking advantage of an optimal condition for cell growth.

Use of oxygen-scavenging systems: Using oxygen-scavenging systems, such as catalase, can help to remove excess oxygen and prevent oxygen toxicity in the culture. This method is relatively low cost compared to other strategies.

Consider changing the carbon source: Using a different carbon source, such as a combination of glucose and another carbon source, can help to overcome diffusion limitation. This may improve the rate of diffusion and cell growth.
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