The invention relates to an enzyme donor conjugate of beta-galactosidase and application of the enzyme donor conjugate in glycocholic acid detection. Specifically speaking, the invention provides a glycocholic acid detection kit which comprises a first reagent, a second reagent and an optional glycocholic acid calibration product or quality control quality. The first reagent comprises an enzyme receptor of the beta-galactosidase and glycocholic acid specific antibodies; the second reagent comprises a glycocholic acid derivative and the enzyme donor conjugate. In the glycocholic acid detection kit, the glycocholic acid derivative is directionally coupled to a specific amino acid residue of an enzyme donor. When the enzyme receptor is combined with the enzyme donor, the enzyme activity can be played, and the combination is controlled by the content of glycocholic acid in a sample. Compared with a radioimmunodetection method, the detection reagents provided by the glycocholic acid detection kit are high in sensitivity, high in specificity and accurate in detection result and do not relate to the isotope contamination problem.
Enzyme donor conjugate of beta-galactosidase and application of enzyme donor conjugate in glycocholic acid detection