An annular array light source illumination-based three-dimensional total internal reflection microscopic imaging device and method. The device comprises: an annular LED array, a conical lens, an object lens, a tubular lens, and an image collecting module. A sample is placed on the conical lens. The annular LED array comprises M single-layered LED arrays of different radii. The lateral surface of the conical lens consists of M round mesa lateral surfaces inclined at different angels. Individual LED lights of each annular LED array sequentially transmit illuminating beams, which are introduced into the conical lens and generate a fading field at the interface between the conical lens and the sample. Scattered lights generated by the sample when illuminated by the fading field are received by the image collecting module sequentially via the object lens and the tubular lens. The annular array light source illumination-based three-dimensional total internal reflection microscopic imaging device and method provided in the present invention implement super-resolution imaging without requiring fluorescent labels.
ANNULAR ARRAY LIGHT SOURCE ILLUMINATION-BASED THREE-DIMENSIONAL TOTAL INTERNAL REFLECTION MICROSCOPIC IMAGING DEVICE AND METHOD