PROBLEM TO BE SOLVED: To provide methods for isolating cell populations of midbrain dopaminergic neuronal progenitor cells derived from stem cells usable in cell therapies for treating Parkinson's disease and in pharmacological assay methods as a substrate.SOLUTION: The invention provides a method for isolating a cell population using a novel combination of markers. The method comprises introduction of a population of midbrain dopaminergic neural progenitor cells into a cell culture medium, and isolation of a cell population of neural progenitor cells expressing Corin and one or more additional markers selected from orthodenticle homeobox 2 (Otx2) and Frizzled-5 (Fzd5) to identify neural cells expressing Corin and one or more additional cell markers as the mDA neural cells.SELECTED DRAWING: Figure 10
NEURONAL PROGENITOR CELLS AND USE METHODS