To provide methods for producing dopamine-producing neural progenitor cells suitable as a therapeutic agent for Parkinson's disease.SOLUTION: Provided is a method for producing dopamine-producing neural progenitor cells, comprising producing a cell population containing Corin and/or Lrtm1-positive cells by the following steps (1) and (2), collecting Corin and/or Lrtm1-positive cells from the resulting cell population using a substance that binds to Corin and/or a substance that binds to Lrtm1, and performing suspension-culture of the Corin and/or Lrtm1-positive cells in a culture medium containing one or more neurotrophic factors. (1) A step of performing adhesion-culture of pluripotent stem cells in the absence of feeder cells in the presence of extracellular matrix in an undifferentiated maintenance medium containing a Sonic hedgehog (SHH) signal stimulant and an undifferentiated maintenance factor; and (2) a step of culturing the cell population obtained in step (1) in a culture medium containing one or more differentiation-inducing factors.SELECTED DRAWING: Figure 1
METHOD FOR PRODUCING DOPAMINE-PRODUCING NEURAL PROGENITOR CELLS