The invention discloses a construction method and application of a nondestructive monitoring neuroinflammation mouse model capable of breaking through skull restriction. After novel luciferase AkaLucand yellow fluorescent protein Venus genes are connected in series, an IRES sequence is connected behind a Gfap gene to construct a Gfap-IRES-Venus-AkaLuc knockin mouse model, and on the basis, the rise of fluorescence intensity in the mouse model of virus-induced neuroinflammation is detected dynamically in a nondestructive manner through intracranial injection of the mouse model with virus-induced neuroinflammation. It is proved that the mouse model constructed through the method can be applied to nondestructive dynamic detection of neuroinflammation, and the mouse model constructed for thefirst time has potential application value in neuroinflammation research.
Construction method and application of nondestructive monitoring neuroinflammation mouse model capable of breaking through skull restriction