The present invention relates to a protein pair for monitoring an interaction between tau proteins in living cells, a base sequence pair encoding the same, a vector pair comprising the same, cell lines transformed by the vector pair, and a method for monitoring tau-tau interaction in living cells using the cell lines, and, more specifically, to a bimolecular fluorescence complementary amino acid sequence pair for monitoring an interaction of intracellular tau proteins, a base sequence encoding the same, a vector pair comprising the same, cell lines transformed by the vector pair, and a method for monitoring tau-tau interaction in living cells using the cell lines, in which the bimolecular fluorescence complementary amino acid sequence pair consists of: a first fluorescence complementary amino acid sequence in which the N-terminal sequence of a Venus amino acid sequence is bound to the C-terminal of a full-length human tau amino acid sequence; and a second fluorescence complementary amino acid sequence in which the C-terminal sequence of the Venus amino acid sequence is bound to the C-terminal of the full-length human tau amino acid sequence. The present invention can monitor and quantify a tau oligomerization process by directly visualizing tau-tau interaction in living cells, and thus may be used as a useful means to investigate the occurrence of tau-associated diseases and to develop a method for preventing and reversing the oligomerization process.
BIMOLECULAR FLUORESCENCE COMPLEMENTARY AMINO ACID SEQUENCE PAIR FOR MONITORING INTERACTION OF INTRACELLULAR TAU PROTEIN