INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 04 October 2018 (04.10.2018) W I PO I PCT omits I iolo DID HE MEM EH 011 (10) International Publication Number WO 2018/178080 Al (51) International Patent Classification: G01N 33/50 (2006.01) GO1N 33/68 (2006.01) (21) International Application Number: PCT/EP2018/057781 (22) International Filing Date: 27 March 2018 (27.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 17163426.4 28 March 2017 (28.03.2017) EP (71) Applicant: JANSSEN VACCINES & PREVENTION B.V. [NL/NL]; Archimedesweg 4, 2333 CN Leiden (NL). (72) Inventors: CRESPO RODRIGUEZ, Rosa; Archimedesweg 4-6, 2333 CN Leiden (NL). APETRI, Constantin, Adrian; Archimedesweg 4-6, 2333 CN Leiden (NL). (74) Agent: MANTEN, Annemieke et al.; Archimedesweg 4-6, 2333 CN Leiden (NL). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a 11 patent (Rule 4.17(ii)) of inventorship (Rule 4.17(iv)) 0 Published: O with international search report (Art. 21(3)) 00 N 1-1 (54) Title: METHODS FOR DETECTION OF TAU PROTEIN AGGREGATION MODULATING COMPOUNDS 0 (57) : The present invention provides a method for the in vitro formation and/or detection of paired helical filaments (PHF) \' of Tau protein, comprising incubating a mixture comprising a Tau protein preparation and a polyanionic co-factor for a pre-determined 0 period of time under conditions that promote the formation of PHFs.
METHODS FOR DETECTION OF TAU PROTEIN AGGREGATION MODULATING COMPOUNDS