To provide methods and systems for generating medial ganglionic eminence (MGE) precursor cells in vitro as well as compositions of enriched MGE precursor cells.SOLUTION: Provided is a method for providing a cell culture in which primate MGE precursor cells are enriched, the method comprising: providing primate pluripotent stem cells into a serum-free medium; and introducing into the medium a plurality of factors including a) a sonic hedgehog pathway activator, b) an SMAD inhibitor, c) a wnt pathway inhibitor, and d) a neural induction supplement, the serum-free medium containing no knockout serum replacement, the cell culture to be provided having enriched MGE precursor cells compared with a cell culture not treated with a combination of factors a) to d), and the MGE progenitor cells expressing NKX2.1 but no PAX6.SELECTED DRAWING: Figure 1
IN VITRO PRODUCTION OF MEDIAL GANGLIONIC EMINENCE PRECURSOR CELLS