It is possible to more appropriately perform fluorescence separation. An information processing apparatus according to an embodiment includes: a fluorescence signal acquisition unit (112) that acquires a plurality of fluorescence spectra corresponding to each of a plurality of excitation lights having different wavelengths and irradiated to a fluorescence stained specimen (30), the fluorescence stained specimen (30) being created by staining a specimen (20) with a fluorescence reagent (10); a link unit (131) that generates a linked fluorescence spectrum by linking at least parts of the plurality of fluorescence spectra to each other in a wavelength direction; a separation unit (132) that separates the linked fluorescence spectrum into spectra for every fluorescent substance using a reference spectrum including a linked autofluorescence reference spectrum in which spectra of autofluorescent substances in the specimen are linked to each other in the wavelength direction and a linked fluorescence reference spectrum in which spectra of fluorescent substances in the fluorescence stained specimen are linked to each other in the wavelength direction; and an extraction unit (132) that updates the linked autofluorescence reference spectrum using the spectra for every fluorescent substance separated by the separation unit.
INFORMATION PROCESSING APPARATUS AND MICROSCOPE SYSTEM