An analytical method based on superparamagnetic nanoparticles includes providing a sample having an analyte in a sample matrix; providing an immediate detection chip having analysis regions, each analysis region being a stationary phase having at least one or more portions; labeling each of the analytes with superparamagnetic nanoparticles, and immobilizing the labeled analytes in the stationary phase; providing an analysis device having means for exciting the superparamagnetic nanoparticles in vitro and means for sensing, receiving and transmitting a response of the excited superparamagneticnanoparticles; placing the chip in the analysis equipment, and exciting the superparamagnetic nanoparticles in vitro; sensing, receiving, and transmitting the response of the superparamagnetic nanoparticles; and analyzing the response and determining a characteristic of the analyte, wherein the response of the superparamagnetic nanoparticles includes harmonics. The invention also provides a hybridinstant detection chip and an analyzer used in the analysis method.
Superparamagnetic particle imaging and application thereof in quantitative multiplexing stationary phase diagnostic determination