The invention relates to the technical field of dental pulp stem and fine treatment, in particular to a method for treating cerebral ischemia reperfusion injury by using a dental pulp stem cell exosome. The method comprises the steps of extraction of third molars free of dental caries and periodontal lesions from healthy volunteers at age of 18 to 30 years, disinfection of tooth surface with 75% alcohol, removal of dental pulp from dental drill, cutting into 3 pieces with 1 mm, flushing three times with PBS containing 2.5% antibiotic, digestion of endodontic tissue in 1.5 ml of EP tubes with 3 mg/ml collagenase type I,4 mg/mL dispersozyme placement at 37 DEG C for 30 minutes,cell resuspension and culturing in a 5% CO2 incubator at 37 DEG C; brain tissue is an organ most sensitive to ischemia and hypoxia of a body, irreversible brain injury occurs after blood flow supply is interrupted for several minutes, animal experiments find that the exosome from the dental pulp stem cells can reduce the cerebral infarction area after cerebral ischemia-reperfusion injury of a mouse. And a key technology is provided for clinical application of the dental pulp stem cell exosome in treatment of cerebral ischemia-reperfusion injury.
Method for treating cerebral ischemia-reperfusion injury by using dental pulp stem cell exosome