A cell image analysis method and a cell image analysis device are provided. A shape of a cell is favorably observed in a non-invasive manner. One aspect of the cell analysis device according to the present invention includes: holographic microscopy 10, an image generation unit 23,24 configured to generate a phase image of a cell based on hologram data acquired by observing the cell with the holographic microscopy, a first machine learning model storage unit 251 configured to store a cell area machine learning model generated by performing machine learning using training data in which a phase image of a cell generated based on the hologram data is used as an input image and a pseudo cell area image based on a stained image acquired by staining a cytoskeleton corresponding to the phase image is regarded as a ground truth image, and a cell area estimation unit 25 configured to output a cell area estimation image indicating a cell area using a machine learning model stored in the first machine learning model storage unit, wherein the phase image generated by the image generation unit for the analysis target cell is used as an input image.
CELL IMAGE ANALYSIS METHOD AND CELL IMAGE ANALYSIS DEVICE