Human cell lines are produced by immortalising a human undifferentiated or precursor cell of a given tissue type using an immortalising agent which includes or has an associated control element whereby activation of the control element terminates immortalisation and allows differentiation of the undifferentiated or precursor cell. The immortalised cell is cultured to produce a homogeneous population of human cells and the control element is then activated to terminate immortalisation and activate differentiation. The differentiation of the cells of the population is allowed, to produce fully differentiated cells of the given tissue type.
Producing large population of neural cells and cell lines, typically human that are pleiotropic but will differentiate following specific treatment