The invention relates to a fluorescence-lifetime imaging microscopy method having time-correlated single-photon counting, wherein a sample (36) is periodically excited with excitation light pulses to emit fluorescence photons by means of a pulsed light source (12), wherein a measurement interval is defined between each pair of consecutive excitation light pulses, the fluorescence photons are detected by means of a detector (42) and an analog detector signal representing the detected fluorescence photons is produced, detection times at which the fluorescence photons are detected by the detector (42) within the measurement intervals are determined on the basis of the detector signal, at least one value characterizing the fluorescence decay behavior is determined on the basis of the detection times of the detected fluorescence photons, and imaging is performed on the basis of the characterizing value. The analog detector signal is sampled within each measurement interval in a plurality of sampling intervals and is converted into a series of discrete signal values associated with the individual sampling intervals. On the basis of the series of discrete signal values that belongs to the associated measurement interval, it is determined whether more than a predefined number of fluorescence photons has been detected within the measurement interval. This measurement interval is discarded for the determination of the characterizing value if more than said predefined number of fluorescence photons has been detected.
FLUORESCENCE-LIFETIME IMAGING MICROSCOPY METHOD HAVING TIME-CORRELATED SINGLE-PHOTON COUNTING, WHICH METHOD PERMITS HIGHER LIGHT INTENSITIES