INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 20 September 2018 (20.09.2018) WIP0 I PCT 0111111010 0111 °nolo Oil ioo VIII Immo oimIE (10) International Publication Number WO 2018/167481 Al (51) International Patent Classification: CO7K 16/42 (2006.01) GO1N 33/566 (2006.01) GO1N 33/537 (2006.01) (21) International Application Number: PCT/GB2018/050645 (22) International Filing Date: 14 March 2018 (14.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1704115.3 15 March 2017 (15.03.2017) GB (71) Applicant: OXFORD GENETICS LIMITED [GB/GB]; First Floor, Medawar 1, Robert Robinson Avenue, The Ox- ford Science Park, Oxford Oxfordshire OX4 4GA (GB). (72) Inventors: CAWOOD, Ryan; C/o Oxford Genetics Limit- ed, First Floor, Medawar 1, Robert Robinson Avenue, The Oxford Science Park, Oxford Oxfordshire OX4 4GA (GB). PAYNE, Thomas; C/o Oxford Genetics Limited, First Floor, Medawar 1, Robert Robinson Avenue, The Oxford Science Park, Oxford Oxfordshire OX4 4GA (GB). PARK- ER-MANUEL, Richard; C/o Oxford Genetics Limited, First Floor, Medawar 1, Robert Robinson Avenue, The Ox- ford Science Park, Oxford Oxfordshire OX4 4GA (GB). TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) (74) Agent: DEHNS; St Bride's House, 10 Salisbury Square, London Greater London EC4Y 8JD (GB). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, 1-1 MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, 00 1-1 71' (54) Title: METHOD OF SELECTING FOR ANTIBODIES 1-1 (57) : The present invention relates to a method for identifying specific binding partners (e.g. antibodies or antibody mimetics) which bind to a desired target polypeptide. In particular, the method involves expressing a library of antibodies or antibody mimetics in a population of mammalian cells, wherein each cell in the population of cells displays the target polypeptide on the outer surface of N the cell, and identifying or isolating cells within the population of cells to which antibodies or antibody mimetics are bound.
METHOD OF SELECTING FOR ANTIBODIES