A signal function S(t1, t2) is obtained from a plurality of coherence transfer pathways in a single acquisition by preparing a molecular system in a coherent non-equilibrium state, and alternately and sequentially detecting signals at individual sampling points, in t2, from the plurality of coherence transfer pathways by using gradient refocusing of a new pathway after signal detection at a sampling point in another pathway. The gradient encoding and refocusing of coherence pathways can use inhomogeneous rf-pulses (B1 gradients) or B0 field gradients. The coherence transfer pathways can be sequentially selected in an arbitrary order. <IMAGE>
Multidimensional NMR spectroscopy using switched acquisition time gradients for multiple coherence transfer pathway detection.