PURPOSE: To form a polyacrylamide type polymer gel medium conveniently and safely by composing a kit by combination of two kinds of monomers, a copolymer, and a crosslinking agent. CONSTITUTION: The monomer (a) is a monomer selected from a substituted and unsubstituted acrylamide monomers, the monomer (b) is a monomer pertaining to crosslinking reaction not through free radical starting mechanism, and the copolymer (c) is a water-soluble uncrosslinking nonionic copolymer of uncharged vinyl monomer mixture composed of one or more kind of monomer other than the monomers (a), (b). A crosslinking agent reacting on a functional group of repeating unit of copolymer derived from the monomer (b) is contained in another container in order to constitute a kit. Consequently, a high purity nontoxic copolymer having appropriate reactivity substitutes for neurotoxic acrylamide and bismonomer and gelation takes place while generating heat slightly under presence of oxygen when a crosslinking agent is added immediately before gelation and injection.
KIT FOR ELECTROPHORETIC GEL MEDIUM