Described herein are compositions and methods for treating diseases and disorders of the brain using a non-viral nanoparticle delivery of CRISPR. Disclosed herein are compositions comprising CRISPR-Gold compositions comprising DNA oligonucleotides, RNA-directed nucleases and guide RNAs. The methods include modulating expression of a gene in a cell using said compositions, inducing site-specific DNA cleavage in a cell, and treating a subject having fragile X syndrome caused by increased metabotropic glutamate receptor 5 signalling using the compositions disclosed herein.
CRISPR-NANOPARTICLES AND METHODS OF USE IN BRAIN DISORDERS