INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 26 July 2018 (26.07.2018) WIP0 I PCT IiiimmomiolollmonolomoilimuioloilionovoimIE (10) International Publication Number WO 2018/136823 Al (51) International Patent Classification: A01K 67/027 (2006.01) CO7K 16/00 (2006.01) (21) International Application Number: PCT/US2018/014568 (22) International Filing Date: (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/448,317 (71) Applicant: OPEN MONOCLONAL TECHNOLOGY, INC. [US/US]; 3911 Sorrento Valley Boulevard, Suite 110, San Diego, CA 92121 (US). (72) Inventors: BUELOW, Roland; 2747 Ross Road, Palo Al- to, CA 94303 (US). BRUGGEMANN, Marianne; 2 Sta- tion Road, Fexton, Cambridge CB22 6SA (GB). MA, Biao; Top Floor Flat, 99 Hills Road, Cambridge CB2 1PG (GB). OSBORN, Michael, J.; Jasmine Cottage, Sturmer, Haver- hill, Suffolk CB9 7XF (GB). - ( 74 ) Agent: LORENZ, Todd, A. et al.; Arnold & Porter Kaye Scholer Llp, Three Embarcadero Center, 10th Floor, San Francisco, CA 94111 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) 19 January 2018 (19.01.2018) English English 19 January 2017 (19.01.2017) US - (54) Title: HUMAN ANTIBODIES FROM TRANSGENIC RODENTS WITH MULTIPLE HEAVY CHAIN IMMUNOGLOBULIN LOCI FIC14 (OmniRat 1) Hu BAC6-3 182 kb AsiSI-Ascl _ 10.0 kb Hu BAC3 173 kb Notl D1-1 D7-27 —Tt - (7 - 2;E; - ,1.3 kb Hu-Rat Annabel 193 kb Notl W O 20 18/1368 23 Al op Ov1 6y2b HC1 4 (Osborn et at, JO HC30 HC14/1-1C30 occasionally found in pale color t t tt tt .t tt t t t t tttt t t t tt ft t tf t t t t tttt t FIG. IA (57) : The invention relates to transgenic animals useful for optimal production of functional immunoglobulins with human idiotypes.
HUMAN ANTIBODIES FROM TRANSGENIC RODENTS WITH MULTIPLE HEAVY CHAIN IMMUNOGLOBULIN LOCI