A retroviral reverse transcriptase having RNA-dependent DNA polymerase activity which comprises at least one mutation at an amino acid position corresponding to amino acids selected from: (a) tyrosine 64 of M-MMLV reverse transcriptase; (b) arginine 116 of M-MMLV reverse transcriptase; (c) lysine 152 of M-MMLV reverse transcriptase; (d) threonine 197 of M-MMLV reverse transcriptase; (e) glutamine 190 of M-MMLV reverse transcriptase; (f) phenylalanine 309 of M-MMLV reverse transcriptase; wherein the retroviral reverse transcriptase is not derived from a human immunodeficiency virus; the reverse transcriptase with properties selected from the group consisting of: (a) increased fidelity in comparison to the corresponding wild-type enzyme; (b) reduced terminal deoxynucleotidyl transferase activity n comparison to the corresponding wild-type enzyme; and (c) reduced RNAse H activity in comparison to the corresponding wild-type enzyme; and method for producing the reverse transcriptase, for amplifying a nucleic acid molecule and for sequencing a nucleic acid molecule.
High fidelity reverse transcriptases and uses thereof