In one embodiment, the invention relates to a method for generating several single-color images from a multicolor image of a sample or object defined by intensity pixels (I<SUB>a</SUB>(x,y), I<SUB>ß</SUB>(x,y), I<SUB>?</SUB>(x,y)) of at least two color channels (a, ß, ?) in order to identify properties of structures of the object or sample or/and identify inherent colors of the object or sample or colors added thereto by a coloring treatment. The single-color images are defined by intensity pixels of only one of the color channels, intensity pixels of several of the color channels having the same intensity ratio among the color channels for all intensity pixels, or intensity pixels of only one resulting color channel that corresponds to a defined combination of the color channels. The multicolor image at least for the intensity pixels of at least one group of intensity pixels is based on an overlay of overlay contributions that are assigned to different original colors, especially at least approximately additive intensity contributions or intensity percentages or/and at least approximately subtractive intensity contributions or intensity percentages. According to the invention, the single-color images represent overlay contributions allocated to different original colors and are generated based on hypothetical or predefined characteristic intensity ratios or characteristic intensity ratios obtained from a calibration or derived from the multicolor image, said characteristic intensity ratios representing ratios between at least two intensity contributions or intensity percentages which are assigned to another one of the color channels, respectively, and are associated with the same property or structure of the object or sample or the same color of the object or sample.
SEPARATION OF SPECTRALLY OVERLAID OR COLOR-OVERLAID IMAGE CONTRIBUTIONS IN A MULTICOLOR IMAGE, ESPECIALLY TRANSMISSION MICROSCOPIC MULTICOLOR IMAGES