A method for super-resolution imaging of interactions between a non-fluorescent species and a material, comprising: (i) contacting the material with a fluorescent species, wherein the fluorescent species selectively interacts with specific sites in the material; (ii) inducing fluorescence in the fluorescent species, and measuring a first fluorescence signal over an area of the material to provide a first quantified distribution map of the fluorescent species in the material; (iii) further contacting the material with a competitive non-fluorescent species that selectively interacts with the same specific sites as the fluorescent species; (iv) measuring a second fluorescence signal of the fluorescent species over an area of the material to provide a second quantified distribution map of the fluorescent species in the material; and (v) calculating the difference in intensity, wavelength, or blinking frequency between the first and second fluorescence signals over the area of material to provide a difference quantified distribution map.
SUPER-RESOLUTION OPTICAL IMAGING OF NON-FLUORESCENT SPECIES