Disclosed is a method for identifying cell-type specific EV markers comprising culturing induced pluripotent stem cells (iPSCs) to provide a culture of a first differentiated cell type; isolating extracellular vesicles (EV)s from the culture of the differentiated cell type; generating a microscopic RNA (miRNA) expression profile for the differentiated cell type by next-generation sequencing; and identifying specific miRNA markers associated with the differentiated cells. Also dislcosed are methods for in vitro testing for a disease condition comprising providing a biological sample; isolating extracellular vesicles from said sample by labeling the extracellular vesicles using fluorescence in situ hybridization, and sorting the labeled extracellular vesicles by high resolution flow cytometry; and determining the status of exosomes and/or an exosome-associated marker in the sample, wherein an abnormal status of exosomes and/or the exosome-associated marker in the sample indicates the presence of a disease condition.
SORTING CELL-TYPE SPECIFIC EXTRACELLULAR VESICLES