Tumor Necrosis Factor Muteins Human TNF muteins having higher binding affinity for human p75-TNF receptor than for human p55-TNF receptor include muteins having at least one deffect amino acid relative to wild-type human TNF at a position corresponding to position 33, 65, 67, 75, 87, 143, 145 or 147 of the wild-type amino acid sequence. Such muteins can be used to characterise human p75-receptor and, together with known muteins with selective binding affinity for human p55-TNF receptor, can aid in distinguishing between TNF functions mediated via binding to human p75-receptor and those mediated via binding to human p55-receptor. (Fig. 4 suggested to accompany abstract).
TUMOR NECROSIS FACTOR MUTEINS