Discloses an isolated nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) fusion protein, comprising an NPP1 component, an Fc region of an immunoglobulin and optionally a targeting moiety, wherein a) the NPP1 component comprises the cysteine-rich region and the C-terminus catalytic domain of NPP1 and lacks the N-terminal cytosolic and transmembrane domains, b) the Fc region and the optional targeting moiety, when present, are each located C-terminally to the NPP1 component, and c) the isolated NPP1 fusion protein has NPP1 enzymatic activity.
Npp1 fusion proteins