Abstract Characteristics of proteins, peptides, and/or peptoids can be determined via two-dimensional correlation spectroscopy and/or two-dimensional co-distribution spectroscopies. Spectral data of the proteins, peptides, and/or peptoids can be obtained with respect to an applied perturbation. two-dimensional co-distribution analysis can be applied to generate an asynchronous co-distribution plot for the proteins, peptides, and/or peptoids to define the population of proteins in solution. In the two-dimensional asynchronous plot, a cross peak can be identified as correlating with an auto peak in the two-dimensional correlation synchronous plot associated with aggregation of the proteins, peptides, and/or peptoids. The two-dimensional asynchronous cross peak can be used to determine an order of a distributed presence of spectral intensities with respect to the applied perturbation. For example, for two wavenumbers v1 and v2, the value of the cross peak corresponding to the two wavenumbers can indicate a presence of spectral intensity at v1 relative to the presence of spectral intensity at v2. WO 2017/127679 PCT/US2017/014338 MAb Monomer E ~AggregatesFI.1 0 1 1 1 1______________1'___ 0 5 10 15 20 25 Minutes -4 E- -82 -16 FIG.l1B -20 0 20 40 60 80 100 120 Temperature ['C] 14 R Rec 8: 7.99 mgmL 14 Rec 12: 5.99 mgmL 121 --- Rec16: 3.0mgmL -~ IIJ';.~Rec2l:1.2mgmL 10 1 'i Rec 31:0.48 mg/mLl 4 -1 2- FIG.I1C Hydrodynamic Radius (nm)
Method and system for spectral data analysis