A recombinant pseudorabies virus expressing E2-crimson, a preparation method therefor and an application thereof. A recombinant vector of the recombinant pseudorabies virus expressing E2-crimson is a pBB5-E2-crimson vector comprising the E2-crimson gene. The red fluorescent protein E2-crimson used in the recombinant pseudorabies virus has high fluorescence intensity, fast maturation time, strong transmission capabilities, and no cytotoxicity to bacteria and mammalian cells. The prepared recombinant pseudorabies virus can efficiently reversely label neural circuits in mouse striatum, has high expression efficiency and strong infectivity, and has broad application prospects in the field of neural circuit tracing.
RECOMBINANT PSEUDORABIES VIRUS EXPRESSING E2-CRIMSON, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF