Embodiments of this invention include image-based systems and methods for detection of one or more biological molecules. A matrix has identifiable analyte-specific capture particle(s) suspended therein, not all being in the same detection plane. Analyte-specific binding moieties (e.g., antibodies) capture analytes. Analyte-specific detector molecules with conjugated detection moieties are then attached to the analyte-specific binding moiety forming particle complexes. A computer assisted, image-based detection system captures images of the particle complexes. Using different sets of analyte-specific detector molecules, each set having a characteristic identifiable feature; it is now possible to implement rapid multiplex assays of cytokines. These image-based systems can be used to aid in diagnosis of disease, evaluation of therapy for disease, or laboratory investigation.
Immobilised-Bead Immunomultiplex Cytokine Assay