The present invention relates to a monoclonal antibody specific for hypoxia-inducible factor-la (HIF- la) and its antigen peptides. The inventive monoclonal antibody was produced using these antigen peptides carrying hydroxylated asparagine residues. The monoclonal antibody of the present invention shows a high affinity toward HIF-la peptides hydroxylated at asparagine 803 by FIH-I, but almost no affinity toward non-hydroxylated ones; thus, this antibody has a great potential for detection of enzymatic activity and inhibitor screening and development for FIH-I.
A MONOCLONAL ANTIBODY FOR DETECTION OF HYDROXYLATED ASPARAGINE 803 OF HIF-1 ALPHA AND ANTIGEN PEPTIDE