The invention provides a test method for verifying the ability of silence PHD2 to enhance the paracrine effect of BM-MSCs. The test method comprises the following steps of 1) preparing PHDMSC-CM and MSC-CM, 2) obtaining experimental NEC young rats, 3) performing experimental verification, and 4) processing data, wherein the process of preparing PHDMSC-CM comprises the following procedures of constructing rat PHD2. shRNA on a lentiviral gene transfer vector pGCSIL-GFP, transfecting BM-MSCs by using lentiviruses containing shPHD2-GFP, determining stably transfected BM-MSCs by detecting GFP fluorescence and PHD2 protein expression, carrying out cell sorting and enrichment by using a flow cytometry, conducting further culturing, then performing culturing in a new serum-free basic culture medium for 48 hours, and separating cells from the culture medium to obtain basic PHDMSC-CM containing paracrine substances efficiently expressed by the BM-MSCs. Experiments prove the ability and way of silence PHD2 to enhance the paracrine effect of the BM-MSCs.
Test method for verifying ability of silence PHD2 to enhance paracrine effect of BM-MSCs (bone marrow mesenchymal stem cells)