The invention discloses a method for construction of double-activation subyeast random peptide library for screening dissociating peptide of protein, which belongs to the molecular biology and biopharmaceutics categories. Making use of characteristic that the DNA binding domain (BD) and the activation domain (AD) of the yeast activator of transcription can separate in space, the method consists ofinserting gene X in domain BD of Bridge particles, inserting random 10 peptide library with thioredoxin as framework in second conditional methilanin promoter (PMET25) downstream and inserting gene Yin pACT2 containing domain AD, co-transforming yeast host cell, finally screening small peptide molecule which can promote X-Y dissociation by using the expression of auxotrophic gene and the corresponding narrative gene. The method provides a new approach for studying dissociation of protein complex; the obtained peptide of protein can effectively block up the important protein complex in the signal path, which provides a target point for medicines which can cure diseases caused by abnormal signal transduction path.
Construction method for double-promoter yeast random peptide library for screening protein dissociation peptide