To provide methods for humanizing non-human antibodies using scoring matrixes based on structures.SOLUTION: Disclosed is a method comprising the following steps of: (a) aligning the amino acid sequence of a non-human heavy chain or light chain variable domain to a first humanized variant of the non-human heavy or light chain domain; (b) identifying aligned framework positions in which the non-human heavy chain or light chain variable domain and the first humanized variant of the non-human heavy chain or light chain variable domain have different amino acid residues, which, due to the difference, influence antigen binding and/or three-dimensional structure of the variable domain; (c) modifying the first humanized variant of the non-human heavy chain or light chain variable region by substituting the amino acid residues identified in the step (b); (d) preparing a nucleic acid sequence encoding the modified variable domain, thereby preparing a nucleic acid sequence encoding a humanized immunoglobulin variable domain.SELECTED DRAWING: None
THREE-DIMENSIONAL STRUCTURE-BASED HUMANIZATION METHODS