PROBLEM TO BE SOLVED: To provide a method of determining the proximity, or changes in the proximity, of two or more molecular features labeled with fluorophores capable of undergoing DARET.SOLUTION: A first molecular feature labeled with a donor fluorophore having a first absorption spectrum and a first emission spectrum is brought into contact with a second molecular feature labeled with an acceptor fluorophore having a second absorption spectrum and a second emission spectrum, where the second absorption spectrum overlaps with the first emission spectrum, and changes in fluorescence polarization of the sample are determined. Thereby, the proximity, or changes in the proximity, of the molecular features can be monitored in real time.
ASSAYS OF MOLECULAR OR SUBCELLULAR INTERACTIVITY USING DEPOLARIZATION AFTER RESONANCE ENERGY TRANSFER (DARET)