The invention discloses an ALK fusion gene detection and typing kit based on sandwich method high-resolution melting curve analysis. A sandwich sequence target segment is amplified in a segmented manner by adding a low GC content joint at the 5' end of a gene-specific primer, and is a fusion gene fragment comprising a high and low GC content consensus sequence and a variable sequence. A high-resolution melting curve general analysis method can judge presence or absence of the fusion gene, and the fluorescence intensity-temperature second-order derivative curve is combined with a key parameteranalysis method to achieve digital judgment of the fusion gene type. The kit solves the problems of high cost, long cycle and difficult typing of the existing lung cancer ALK fusion gene detection, integrates the advantages of two technologies of real-time quantitative reverse transcription PCR and high-resolution melting curve analysis, and can be applied to fusion gene detection of fresh tissues, paraffin-embedded tissues, pleural effusion and other types of specimens.
ALK fusion gene detection and typing kit based on sandwich method high-resolution melting curve analysis