An imaging device and method for quickly acquiring large-sample three-dimensional structure information and molecular phenotype information. The apparatus comprises: a sample storage device; a three-dimensional mobile station (9) for driving the sample storage device to move within a three-dimensional space; a vibration slicing module (8) for slicing the sample (5) so that the sample obtains a superficial-layer portion; and a wide-field optical microimaging module for performing high-throughput tomography on the superficial-layer portion of the sample. By means of the device and method, the disadvantages in the prior art that a sample preparation flow is complex, a sample form and a fluorescence signal are affected and an imaging speed is slow are solved, and three-dimensional structure information about a sample can be quickly acquired and analyzed, wherein the acquired data has an auto-registration characteristic, molecular phenotype dyeing is performed on a sample slice in a position of interest to obtain molecular phenotype information about the sample, and same can be registered into the acquired three-dimensional structure information.
IMAGING DEVICE AND METHOD FOR QUICKLY ACQUIRING LARGE-SAMPLE THREE-DIMENSIONAL STRUCTURE INFORMATION AND MOLECULAR PHENOTYPE INFORMATION