The invention discloses an STED (Stimulated Emission Depletion Microscopy) super-resolution imaging probe. Organic molecules of an OPV (Oligomeric Phenylene Vinylene) framework form a core-shell structure after being coated with silicon dioxide, and coated nano particles (40-60 nm) are uniform in size and smooth in surface and have high fluorescence intensity. The maximum stimulated radiation erasing efficiency can reach 99%, meanwhile, the resolution ratio of about 60 nm can be achieved at low power, real-time dynamic monitoring of physiological activities of organisms can be considered whilesub-cellular organelles are visualized, and therefore the fluorescent probe is a novel fluorescent probe capable of being used for STED super-resolution imaging.
STED super-resolution imaging fluorescent probe