Technologies for identifying one or more neoepitope peptides include generating a database that includes peptide sequences within a predefinited range of length of residues, assigning a prior probability to each of the peptide sequences in the database, obtaining mass spectra of a plurality of fragments of a target molecule produced by mass spectrometry, determining, for each mass spectrum, matching scores of peptide-spectrum matches between the mass spectra and the peptide sequences in the database as a function of prior probabilities of the peptide sequences and matching probabilities, and determining a subset of the peptide-specturm matches that has a corresponding matching socre higher than a threshold.
CONSTRAINED DE NOVO SEQUENCING OF NEO-EPITOPE PEPTIDES USING TANDEM MASS SPECTROMETRY