PROBLEM TO BE SOLVED: To provide anti-Notch1 NRR antibodies, compositions comprising the antibodies, and methods of using the antibodies.SOLUTION: There is provided an isolated anti-Notch1 negative regulatory region (NRR) antibody that binds to a heterodimerization domain (HD) of a Notch1 NRR. When bound to the Notch1 NRR, the antibody decreases Notch1 signaling. The Notch1 HD is a human Notch1 HD with specific amino acid residues or a murine Notch1 HD with specific amino acid residues. Also provided is a method of producing the anti-Notch1 antibody.SELECTED DRAWING: None
ANTI-NOTCH1 NRR ANTIBODIES AND METHODS OF USING THE SAME