INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 09 August 2018 (09.08.2018) WIP0 I PCT ill 1111u°11101llioIIl °nolo DID mil HE Elmo mnio oimIE (10) International Publication Number WO 2018/141894 Al (51) International Patent Classification: CO7K 16/28 (2006.01) (21) International Application Number: PCT/EP2018/052624 (22) International Filing Date: 02 February 2018 (02.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 17154388.7 02 February 2017 (02.02.2017) EP (71) Applicant: MERCK PATENT GMBH [DE/DE]; Frank- furter StraBe 250, 64293 Darmstadt (DE). (72) Inventors: RUKER, Florian; Montigasse 6, 1170 Vien- na (AT). BONISCH, Maximilian; Boerhaavegasse 3/2/10, 1030 Vienna (AT). (74) Agent: REDL, Gerda et al.; REDL Life Science Patent At- torneys, Donau-City-StraBe 11, 1220 Vienna (AT). = (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). — Published: with international search report (Art. 21(3)) 00 (54) Title: PREFERRED PAIRING OF ANTIBODY DOMAINS 1-1 0, - -- 13 (57) : An antigen-binding molecule (ABM) comprising a cognate LC/HC dimer of an antibody light chain (LC) composed of a VL and a CL antibody domain, associated to an antibody heavy chain (HC) comprising at least a VH and a CH1 antibody domain, O which association is through pairing the VL and VH domains and the CL and CH domains, wherein the amino acids at the position 18 N in the CL domain and at the position 26 in the CH1 domain are of opposite polarity, wherein numbering is according to the IMGT.
PREFERRED PAIRING OF ANTIBODY DOMAINS