A neuropeptide is caged and delivered to a synaptic site, then photolytically activated to enable precise temporal and spatial temporal investigation of localized signaling activity or synaptic responses. Caged molecules, delivery and activation systems clarify the kinetics and activity of neuropeptide-mediated signaling to identify agonists and permit focused drug evaluation for specific diseases and conditions. The caged peptides may also provide light-mediated treatment therapies for localized relief of pain or nerve-related conditions, and serve as a screening or measurement tool for potential agonists and antagonists. A kit includes the caged molecule and may further include a reporter for indicating response to the activated neuropeptide.
Caged Neuropeptides: modulation and measurement of synapse and signaling activity; and methods for drug evaluation, pharmaceuticals preparation and treatment