A two-photon probe for real time monitoring of intracellular calcium ions is provided to realize high two-photon cross section for obtaining clear two-photon microscopy image at the low probe concentration, to show high selectivity to Ca2+ ion, and to distinguish Intracytoplasmic probe and membrane-bound probe. A two-photon probe for real time monitoring of intracellular calcium ions has a structure indicated as the chemical formula (1). In the chemical formula (1), R1 is CH3 or H; R2 is the chemical formula (1-a) or the chemical formula (1-b); and R3 is H or CH2OCOCH3. The two-photon probe is obtained by reacting a compound of the following chemical formula (2) and a compound of the following chemical formula (3). In the chemical formula (2), R4 is NH2 or CHO and R5 is H or CH3.
Two photon probe for real time monitoring of intracellular calcium ions, method for preparing the same and method for real time monitoring of intracellular calcium ions