The present invention relates to a method of preparing a primer used for determining the IVS7-2AG site mutation involved in the large vestibular aqueduct syndrome. The method comprises the steps of : designing a primer pair which may introduce any base substitutive mutation in the region of 1-13 bases between upstream and downstream including the IVS7-2 mutation site (AG) based upon the IVS7-2 A G mutation site in the PDS gene, so as to obtain a new restriction site which may be used for distinguishing IVS7-2 wild type A site and mutant G site in the amplification products. The present invention further relates to the primers prepared according to this method, the kit or reagent or identical product comprising the primers, and a method for determining the IVS7-2AG site mutation of large vestibular aqueduct syndrome with said primers in vitro.
A METHOD FOR PREPARING THE PRIMERS FOR IN VITRO IDENTIFYING MUTATIONS WITHIN LARGED VESTIBULAR AQUEDUCT SYNDROMIC HEARING LOSS PDS GENE AND THE USE THEREOF