A two-photon stimulated emission depletion composite microscope using continuous light loss, the microscope comprising: a two-photon imaging unit (100) and an STED imaging unit (200), wherein for thicker samples, the two-photon imaging unit (100) can be used, and for regions of interest on the surface of the samples, the STED super-resolution imaging unit (200) can be used. The two-photon stimulated emission depletion composite microscope using continuous light loss integrates two functions, i.e. STED imaging and two-photon imaging, so as to provide a powerful tool for cutting-edge biomedical research.
TWO-PHOTON STIMULATED EMISSION DEPLETION COMPOSITE MICROSCOPE USING CONTINUOUS LIGHT LOSS