The invention discloses a stable isotope-based high-throughput proteomic quantitative reagent, which can also be called an isobaric Labeling (IBT) reagent, and the structure of the stable isotope-based high-throughput proteomic quantitative reagent comprises the formula of reporter group-balance group-activation group. Such reagent can be accomplished by different types of chemical structures. Theinvention discloses eight groups of different chemical structures, and each group of structures can comprise up to 10 label reagents (represented by IBT-10PLEX) with completely identical chemical structures. However, 13C or 15N isotopes (which can be represented by T-114, T-115N, T-115C, T-116N, T-116C, T-117N, T-118N, T-117C, T-118C and T-119 respectively) are contained at different positions ineach label reagent. The invention discloses a preparation method of three groups of isobaric tag reagents, and further discloses a quantitative analysis method of polypeptide by adopting the IBT-10PLEX reagent. The IBT-10PLEX disclosed by the invention adopts a novel chemical structure, the preparation difficulty is reduced, and the yield is improved, so that the product cost can be reduced, andthe application range of an isobaric labeling method is expanded.
Stable isotope-based high-throughput proteomic quantitative reagent