To provide antibodies having an antagonistic action to IL-33, particularly isolated human anti-IL-33 neutralizing monoclonal antibodies or fragments of the antibodies, in which the amino acid sequence in a framework region is an amino acid sequence of a germline or an amino acid sequence of combination thereof.SOLUTION: Epitopes are identified for multiple anti-IL-33 monoclonal antibodies, a human anti-IL-33 neutralizing monoclonal antibody is obtained, further a mutation is introduced into its complementarity determining region, and thereby a complementarity determining region with high binding affinity to IL-33 is identified. By using the identified complementarity determining region, a human anti-IL-33 neutralizing monoclonal antibody in which the amino acid sequence of the framework region is the amino acid sequence of the germline amino acid sequence or a combination thereof is obtained.SELECTED DRAWING: Figure 2
HUMAN ANTI-IL-33 NEUTRALIZING MONOCLONAL ANTIBODY