A rabies virus glycoprotein derived peptide (RDP) is connected to polyarginine to design a brain-targeted nucleic acid delivery carrier modified with the RDP. Mediation into brain is achieved throughinteractions between the RDP and an acetylcholine receptor, a nucleic acid is loaded through an electrostatic function by utilizing positive charges carried by the polyarginine, and physical and chemical properties of the nucleic acid are studied. The cellular uptake efficiency of the RDP is studied and is equivalent to the RVG29 uptake efficiency. RDP-(R)n nanoparticles loading siRNA or plasmid DNA are prepared through complex coacervation, are small and uniform in particle size, are round and complete when observed under a scanning electron microscope, and have a good brain targeting application prospect.
A brain-targeted nucleic acid delivery carrier modified with a rabies virus glycoprotein derived peptide and applications thereof