The present invention discloses real-time fluorescent quantitative PCR detection primers and probes, kit and method for novel coronavirus 2019-nCoV (also called 2019-nCoV). The present invention selects two sites in nucleic acid after 2019-nCoV replication for detection, wherein both of the two sites are specific to 2019-nCoV. The detection kit has high specificity and has no cross-reaction with the total nucleic acid of other common pathogens that have the same site of infection or similar symptom of infection as 2019-nCoV and human white blood cells. The present invention uses a single tube with three fluorescence channels to simultaneously detect the presence of novel coronavirus 2019-nCoV and reference gene Rnase P, which can detect the presence of novel coronavirus 2019-nCoV RNA in alveolar lavage fluid, nasal swabs, throat swabs and other samples. The present invention has a short detection time, which can be as low as 1.5 h, is suitable for rapid detection and diagnosis in clinical settings, with high specificity, high repeatability, high sensitivity and high accuracy for detection of 2019- nCoV viruses.
REAL-TIME FLUORESCENT PCR DETECTION PRIMERS AND PROBES, KIT AND METHOD FOR NOVEL CORONAVIRUS 2019-nCoV