The invention belongs to the fields of optical microscopy and biological cell imaging technologies, and provides a method and a system for super-resolution imaging of deep cells, and a prism optical sheet device. A first technical scheme is characterized in that super-resolution optical fluctuation microscopy (SOFI) and super-resolution localization microscopy (LM) are combined, the super-resolution image of the deep cells can be obtained by eliminating non-associated background noises through computer operation, and the first technical scheme can be directly applied to an ordinary fluorescentmicroscope without modifying the original optical structure. A second technical scheme is characterized in that the prism optical sheet device is loaded to an inverted microscope, background noises are reduced by physical means, the super-resolution image of the deep cells is obtained by the localization microscopy, and the second technical scheme can be directly loaded to a conventional invertedfluorescence microscope.
Method and a system for super-resolution imaging of deep cells, and prism optical sheet device