The present invention relates to chelate-fluorophore conjugates and their use in monitoring binding reactions between a chelate and a macromolecular biological binding agent, such as an antibody. Such monitoring is used both to identify anti-chelate antibodies that have a desired metal selectivity and to configure immunoassays that provide a means of detecting and quantitating a given metal ion.
Materials and methods for measuring chelate: anti-chelate binding by fluorescence polarization immunoassay