To provide new tissue models based on cell culture representing in vivo tissue behavior.SOLUTION: The invention provides an in vitro cultured, artificial three dimensional neuronal tissue culture comprising a heterogeneous cell population of at least two different progenitor and neuronal differentiation layers, where at least one progenitor layer comprises outer radial glia cells, the tissue further comprises cells of the outer or extra cortical subventricular zone and cortical inner fiber layer, the culture comprises a three dimensional matrix. The invention also provides a method for generating such a tissue and a kit for the method.SELECTED DRAWING: None
THREE DIMENSIONAL HETEROGENEOUSLY DIFFERENTIATED TISSUE CULTURE