The invention relates to fusion proteins comprising at least three domains, where a first domain facilitates membrane localization of the fusion protein in a cellular context, and a second domain has or presumably has a ligand-binding function of a nuclear receptor, a third domain has an activity able to activate a signal pathway connected to a Ras protein in a cell, characterized in that when there is a lack of binding or, alternatively, when there is binding of ligand to the second domain the third domain cannot exert its activity to activate a signal pathway connected to a Ras protein in a cell, despite membrane localization. It additionally comprises inter alia cells which comprise such fusion proteins, assay methods using such cells, which are used inter alia for detecting specific interactions between ligand-binding sections of nuclear receptors and ligands, and kits for use in these assays.
Method for the cellular high-throughput-detection of nuclear receptor ligand interactions