Systems and methods for analyzing ratiometric data, e.g., ratiometric image data such as fluorescent image data, may generate a correlation matrix for the ratiometric data, generate a plurality of eigenvalues and a plurality of eigenvectors based on the correlation matrix, select a set of eigenvectors from the plurality of eigenvectors, and reconstruct a set of enhanced ratiometric data for use in analysis.
Methods and systems for analyzing ratiometric data