Phosphoinositides participate in many signaling cascades via phospholipase C stimulation, which hydrolyzes phosphatidylinositol bisphosphate, producing second messengers diacylglycerol and inositol 1,4,5-trisphosphate (InsP3). Destructive chemical approaches required to measure [InsP3] limit spatio-temporal understanding of subcellular InsP3 signaling. Disclosed are new biosensors and test kits which allow studying InsP3 dynamics at high temporal and spatial resolution, thereby understanding InsP3 signaling in intact cells.
Biosensors to measure InsP3 concentration in living cells