Systems and methods are provided for mapping myocardial tissue architecture based on diffusion tensor imaging (DTI). A set of eigenvectors is derived from diffusion tensor data, where each eigenvector describes the diffusion of spins along one of the Cartesian directions. A radial coordinate axis and a circumferential plane are determined based on anatomical information of the subject, such as from an image depicting the epicardial surface of the subject's heart, A longitudinal coordinate axis and a circumferential coordinate axis are determined based on the radial coordinate axis and circumferential plane, A fiber architecture matrix (FAM) is then computed for locations in the subject's heart based on projecting the set of eigenvectors onto a local coordinate system defined by the circumferential, radial, and longitudinal axes, Maps that represent myocardial tissue architecture can then be generated using the FAM for locations within the subject's heart,
MAPPING CARDIAC TISSUE ARCHITECTURE SYSTEMS AND METHODS