INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111111111111111111111111111111111011111101111111111111111111111111111110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\'\' WO 2018/127787 Al 12 July 2018 (12.07.2018) WIP0 1 PCT (51) International Patent Classification: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, C07K 16/28 (2006.01) A61P 35/00 (2006.01) TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, A61K 39/395 (2006.01) A61P 37/04 (2006.01) EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (21) International Application Number: MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, PCT/1B2018/000043 TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (22) International Filing Date: 05 January 2018 (05.01.2018) Published: — (25) Filing Language: English with international search report (Art. 21(3)) — before the expiration of the time limit for amending the (26) Publication Language: English claims and to be republished in the event of receipt of (30) Priority Data: amendments (Rule 48.2(h)) 62/443,281 06 January 2017 (06.01.2017) US (71) Applicant: EUTILEX CO., LTD. [KR/KR]; Suite #1401 Daeryung Technotown 17 Cha, Gasan Digital 1-ro 25, Geumcheon-gu, Seoul 08594 (KR). (72) Inventors: KWON, Byoung S.; c/o Eutilex Co., Ltd., Suite #1401 Daeryung Technotown 17 Cha, Gasan Digital f- _ ro 25, Geumcheon-gu, Seoul 08594 (KR). LEE, Seoung- Joo; c/o Eutilex Co., Ltd., Suite #1401 Daeryung Tech- - notown 17 Cha, Gasan Digital 1-ro 25, Geumcheon-gu, Seoul 08594 (KR). KIM, Young Ho; c/o Eutilex Co., Ltd., Suite #1401 Daeryung Technotown 17 Cha, Gasan Digi- tal 1-ro 25, Geumcheon-gu, Seoul 08594 (KR). OH, Ho- = =_ _ Sik; c/o Eutilex Co., Ltd., Suite #1401 Daeryung Techno- town 17 Cha, Gasan Digital 1-ro 25, Geumcheon-gu, Seoul _ 08594 (KR). LEE, Joong Won; c/o Eutilex Co., Ltd., Suite #1401 Daeryung Technotown 17 Cha, Gasan Digital 1-ro 25, Geumcheon-gu, Seoul 08594 (KR). = Agent: CHANG, Duck Soon et al.; Kim & Chang, Seyang = (74) = B/D, 39 Sajikno-8-gil, Jongno-gu, Seoul 03170 (KR). Designated States (unless otherwise indicated, for every = (81) = kind of national protection available): AE, AG, AL, AM, _ AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, = CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, = = KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, = — MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, = OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, — SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. = Designated States (unless otherwise indicated, for every — (84) - kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, IN GO Ir ---- (54) Title: ANTI-HUMAN 4-1 BB ANTIBODIES AND USE THEREOF IN \' (57) : Provided are anti-human 4-1BB antibodies and fragments thereof with one or more structural features that are not Il ----. found in a reference anti-human 4- 1BB antibody, where said features may improve certain characteristics of the antibody relative to GO Il a reference antibody. Various in vitro and in vivo methods and reagents related to anti-human 4-1BB antibodies described herein are © also provided. Methods include, for example, inducing T-cell proliferation, inducing T cell secretion of IFNy, as well as detection, N prevention, and/or therapeutic treatment of cancer using an anti-human 4-1BB antibody or fragment thereof. O
ANTI-HUMAN 4-1 BB ANTIBODIES AND USE THEREOF