The invention discloses an extraction method of messenger RNA. According to the extraction method, plasmids of marked ribosome are transfected into an embryo through an intrauterine embryo electrotransfection technology; and after the plasmids grow for a specific time, a translated ribosome affinity purification technology is performed to obtain purified messenger RNA. According to the invention,the intrauterine embryo electrotransfection technology and the translated ribosome affinity purification technology are combined. Specific neurons are marked at specific time and in a brain area according to time characteristics of neuron development in different lamellar layers of cerebral cortex, and experiments are performed at different time points in the development process, so that a development period dynamic molecular library is expected to be obtained to construct a translation spectrum, and a solid foundation is laid for summarizing rules, discovering specific key factors and screening effective genes.
Extraction method of messenger RNA