The present disclosure discloses methods and devices to obtain physiological measurements of microengineered physiological systems including microscale organotypic models of in vitro nerve tissue that mimics clinical nerve conduction and nerve fiber density (NFD) tests. The results obtained from the use of these methods and devices are better predictive of clinical outcomes, enabling a more cost-effective approach for selecting promising lead compounds with higher chances oflate-stage success. The disclosure includes the fabrication and utilization of a three-dimensional microelectrode arrays on microengineered system that enables the growth of a uniquely dense, highly parallel neural fiber tract.
MICROELECTRODE ARRAY AND USES THEREOF