['pernits', 'rapidly', 'drawn', 'blood', 'samples,', 'which', 'minimize', 'stress-evoked', 'elevations', 'of', 'corticosteroids', 'in', 'the', 'plasma.', 'Such', 'rapidly', 'drawn', 'samples', 'are', 'essential', '2', 'for', 'authentic', 'stress', 'studies,', 'Obtaining', 'Urine', 'Samples', 'from', 'Squirrel', 'Monkeys.', ' ', 'To', 'obtain', 'urine', 'samples', 'suitable', 'for', 'stress-metabolic', 'studies,', 'we', 'have', 'designed', 'a', 'metabolic', 'cage', 'that', 'is', 'suitable', 'for', 'squirrel', 'monkeys.', 'This', 'device', 'permits', 'a', 'lov-stress', 'isolation', 'of', 'the', 'monkey', 'and', 'allows', 'urine', 'samples', '_', 'to', 'be', 'obtained', 'that', 'are', 'relatively', 'uncontaminated', 'by', 'feces', 'or', 'food', 'particles.', 'Food', 'and', 'water', 'are', 'provided', 'ad', 'libitum,', 'making', 'it', 'possible', 'to', 'carry', 'out', 'metabolic', 'studies', 'lasting', 'several', 'days,', 'Urine', 'samples', 'are', 'collected', 'at', 'periodic', 'intervals', 'and', 'placed', 'in', 'frozen', 'storage.', 'Subsequently', 'they', 'are', 'thawed,', 'the', 'volume', 'determined,', 'and', 'the', 'aamples', 'centrifuged', 'éo', 'remove', 'solid', 'particles', 'and', 'sediment.', 'Corticoid', 'assays', 'are', 'then', 'performed,', 'keeping', 'the', 'samples', 'frozen', 'between', 'assays.', 'Corticosteroid', 'Analysis', 'of', 'Squirrel', 'Monkey', 'Plasma', 'and', 'Urine.', ' ', 'For', 'the', 'initial', 'analysis', 'of', 'monkey', 'plasma', 'and', 'urine', 'samples,', 'we', 'have', 'used', 'the', 'methodology', 'developed', 'in', 'our', 'laboratories', 'for', 'mouse', 'plasma', 'assays.', 'Thic', 'is', 'a', 'highly', 'sensitive', 'fluorescence', 'method', 'requiring', 'sample', 'volumee', 'ef', 'only', '50', 'jl.', 'For', 'assay', 'of', 'the', 'intact', 'plasma', 'or', 'urine,', 'the', 'sample', 'is', 'extracted', 'with', 'chloroform', 'and', 'the', 'fluorescence', 'developed', 'by', 'reaction', 'with', '.', 'sulfuric', 'acid/ethanol', 'reagent.', 'After', 'incubation,', 'the', 'fluorescence', 'is', 'é', '"determined', 'in', 'a', 'spectreflucrometer', 'that', 'has', 'been', 'modified', 'to', 'accept', 'very', 'small', 'cuvettes', 'containing', '0.4', 'ml', 'samples.', 'In', 'the', 'assay', 'of', 'the', 'intact', 'sample,', 'the', 'procedure', 'is', 'highly', 'specific', 'for', 'the', 'll-hydroxy', 'corticosteroids,', "'", 'which', 'include', 'corticosterone,', 'curtisvl,', 'aud', 'sume', 'of', 'their', 'metabolites.', ' ', ' ']