['D00', 'P', 'gt', 'b', '£', 'Other', 'grantors:', 'U.', 'S.', 'Public', 'Health', 'Service,', 'Michigan', 'Heart', 'Association,', 'American', 'Medical', 'Association', 'Education', 'and', 'Research', 'Foundation,', 'and', 'Detroit', 'General', 'Hospital', 'Research', 'Corporation.', 'From', 'the', 'Department', 'of', 'Medicine,', 'Wayne', 'State', 'University', 'School', 'of', 'Medicine,', 'Detroit.', 'STABILIZATION', 'OF', 'URINARY', '3-HYDROXYANTHRANILIC', 'ACID', 'BY', 'ORAL', 'ADMINISTRATION', 'OF', 'L-ASCORBIC', 'ACID', 'This', 'paper', 'presents', 'a', 'simple', 'method', 'for', 'the', 'stabilization', 'of', 'urinary', '3-hydroxyanthranilic', 'acid', '(3-HOA).', 'Consecutive', 'series', 'of', 'four', 'overnight', 'specimens', 'of', 'urine', 'were', 'collected', 'from', 'each', 'of', 'five', 'tumor', 'paticnts,', 'After', 'collection', 'of', 'the', 'second', 'specimen,', 'each', 'patient', 'was', 'given', 'L.-ascorbate', 'orally', 'to', 'cause', 'spillage', 'of', 'ascorbate', 'into', 'the', 'urine.', 'Known', 'amounts', 'of', '3-HOA', 'were', 'then', 'added', 'to', 'the', 'urine', 'samples', 'and', 'allowed', 'to', 'incubate', 'at', '37°', 'for', 'six', 'hours.', 'Results', 'showed', 'that', 'in', 'specimens', '!', 'and', '2,', 'collected', 'before', 'ascorbate', 'administration,', 'mean', 'percentage', 'recovery', 'of', 'added', '3-HOA', 'was', 'about', '50%;', 'whereas,', 'in', 'specimens', '3', 'and', '4', '“the', '3-HOA', 'recovery', 'was', 'about', '100%', 'and', 'urinary', 'ascorbate', 'levels', 'were', 'clevated', 'as', 'expected.', 'Since', 'urine', 'ordinarily', 'remains', 'in', 'the', 'bladder', 'for', 'at', 'least', 'six', 'hours,', 'the', 'stabilization', 'of', 'urinary', '3-HOA', 'by', 'oral', 'administration', 'of', 'L-ascorbic', 'acid', 'before', 'collection', 'of', 'urine', 'specimens', 'might', 'give', 'more', 'reliable', 'data', 'concerning', 'the', 'urinary', 'excretion', 'of', '3-HOA', 'by', 'patients', 'with', 'tumors', 'of', 'the', 'bladder.', 'Pipkin,', 'G.', 'E.,', 'Nishimura,', 'R.,', 'Banowsky,', 'L.', 'and', 'Schlegel,', 'J.', 'U.', 'Proceedings', 'of', 'the', 'Society', 'for', 'Experimental', 'Biology', 'and', 'Medicine', '126(3):', '702-704,', '1967.', 'Other', 'grantor:', 'National', 'Cancer', 'Institute.', 'From', 'the', 'Department', 'of', 'Surgery,', 'Section', 'of', 'Urology,', 'Tulanc', 'University', 'School', 'of', 'Medicine,', 'New', 'Orleans.', 'QUANTITATIVE', 'DETERMINATION', 'OF', 'CHLOROGENIC', 'ACID', 'IN', 'PLANT', 'TISSUE', 'BY', 'COMBINED', 'POLYVINYLPYRROLIDONE', 'COLUMN', 'AND', 'GAS', 'CHROMATOGRAPHY', 'A', 'new,', 'specific,', 'and', 'sensitive', 'chromatographic', 'method', 'for', 'the', 'quan-', 'titative', 'determination', 'of', 'chlorogenic', 'acid', 'in', 'plant', 'tissue', 'has', 'been', 'devel-', 'oped.', 'Polyvinylpyrrolidone', 'column', 'chromatography', 'is', 'utilized', 'for', 'pre-', 'liminary', 'quantitative', 'isolation', 'of', 'chlorogenic', 'acid', 'from', 'whole', 'extracts', 'of', 'plant', 'tissue,', 'The', 'partially', 'purified', 'chlorogenic', 'acid,', 'after', 'conversion', 'to', 'the', 'trimethylsilyl', 'derivative,', 'is', 'then', 'further', 'purified', 'and', 'quantitatively', 'deter-', 'mincd', 'by', 'gas', 'chromatography.', 'The', 'precision', 'and', 'accuracy', 'of', 'this', 'method', 'result', 'from', 'the', 'very', 'effective', 'preliminary', 'quantitative', 'separation', 'procedure,', 'including', 'both', 'extraction', 'and', 'column', 'chromatography', 'steps,', 'and', 'from', '.', 'the', 'specificity', 'obtained', 'through', 'the', 'coordinated', 'column', 'and', 'gas', 'chromato-', 'graphy', 'steps.', 'The', 'described', 'procedure', 'was', 'developed', 'specifically', 'to', 'remove', 'all', 'free', 'chlorogenic', 'acid', 'from', 'tobacco', 'tissue.', 'Equally', 'good', 'results', 'have', 'also', 'been', 'obtained', 'in', 'extraction', 'of', 'tomato', 'and', 'bean', 'plant', 'tissue.', 'The', '82', 'method', 'usually', 'requires', 'only', 'a', 'few', 'grams', 'of', 'fresh', 'plant', 'tissue', 'for', 'analysis,', 'and', 'its', 'essential', 'features', 'appear', 'readily', 'applicable', 'to', 'the', 'analysis', 'of', 'other', 'plant', 'phenolic', 'compounds.', 'Wilson,', 'J.', 'L.,', 'Dunlap,', 'W.', 'J.', 'and', 'Wender,', 'S.', 'H.', 'Journal', 'of', 'Chiromatography', '35(3):329-335,', '1968.', 'Other', 'grantor:', 'National', 'Science', 'Foundation.', 'From', 'the', 'Chemistry', 'Department,', 'University', 'of', 'Oklahoma,', 'Norman.', 'A', 'THIN-LAYER', 'CHROMATOGRAPHY-FLUOROMETRY', 'METHOD', '|', 'FOR', 'QUANTITATIVE', 'ANALYSIS', 'OF', 'SCOPOLIN', 'AND', 'SCOPOLETIN', 'IN', 'TOBACCO', 'An', 'improved,', 'relatively', 'rapid', 'and', 'precise', 'quantitative', 'method', 'capable', 'of', 'determining', 'fractional', 'microgram', 'amounts', 'of', 'both', 'scopolin', 'and', 'scopole-', 'tin', 'in', 'tobacco', 'extracts', 'is', 'described.', 'This', 'new', 'procedure', 'has', 'two', 'major', 'advantages', 'over', 'older', 'methods.', 'First,', 'both', 'scopolin', 'and', 'scopoletin', 'can', 'be', 'determined', 'from', 'the', 'same', 'chromatogram,', 'wl}Jlercas', 'many', 'of', 'the', 'other', 'published', 'methods', 'determine', 'only', 'one', 'of', 'these,', 'usually', 'scopoletin.', 'Second,', 'since', 'scopoletin', 'has', 'a', 'fluorescence', 'intensity', 'which', 'is', 'approximately', 'nine', 'times', 'greater', 'than', 'that', 'of', 'scopolin,', 'much', 'smaller', 'amounts', 'of', 'scopoletin', 'can', 'be', 'readily', 'determined', 'under', 'the', 'conditions', 'employed.', 'This', 'represents', 'a', 'distinct', 'advantage', 'since', 'the', 'amount', 'of', 'scopoletin', 'present', 'in', 'properly', 'handled', 'tobacco', 'tissues', 'is', 'usually', 'much', 'smaller', 'than', 'the', 'amount', 'of', 'scopolin', 'present.', 'Winkler,', 'B.', 'C.,', 'Dunlap,', 'W.', 'J.,', 'Rohrbaugh,', 'L.', 'M.', 'and', 'Wender,', 'S.', 'H.', 'Journal', 'of', 'Chromatography', '35(4):570-571,', '1968.', 'Other', 'grantor:', 'National', 'Science', 'Foundation.', 'From', 'the', 'Departments', 'of', 'Chemistry', 'and', 'Botany-Microbiology,', 'University', 'of', 'Oklahoma,', 'Norman.', ')', 'QUANTITATIVE', 'ANALYSIS', 'OF', 'SCOPOLIN', 'AND', 'SCOPOLETIN', 'IN', 'TOBACCO', 'PLANTS', 'TREATED', 'WITH', 'MALEIC', 'HYDRAZIDE', 'Tobacco', 'plants', 'treated', 'with', 'a', 'preparation', 'containing', 'maleic', 'hydrazide', 'were', 'harves(eff', 'at', 'various', 'periods', 'of', 'time', 'and', 'separated', 'into', 'leaves,', 'stems,', 'and', 'roots.', 'The', 'amount', 'of', 'scopolin', 'and', 'scopoletin', 'present', 'in', 'each', 'part', 'was', 'then', 'quantitatively', 'determined', 'by', 'a', 'recently', 'developed', 'thin-layer', 'chro-', 'matographic-fluorimetric', 'procedure', 'and', 'compared', 'to', 'the', 'amount', 'of', 'scopo-', 'lin', 'and', 'scopoletin', 'present', 'in', 'control', 'plants', 'harvested', 'at', 'the', 'same', 'time.', 'Scopolin', 'especially,', 'and,', 'to', 'a', 'much', 'lesser', 'degree,', 'scopoletin', 'were', 'found', 'to', 'increase', 'in', 'stems', 'and', 'leaves', 'of', 'the', 'maleic', 'hydrazide-treated', 'plants', 'as', 'compared', 'with', 'the', 'corresponding', 'controls.', 'The', 'highest', 'levels', 'of', 'scopolin', 'and', 'scopoletin', 'were', 'noted', 'in', 'stem', 'tissue,', 'with', 'the', 'larger', 'increases', 'coming', 'when', 'more', 'than', 'IS', 'days', 'had', 'clapsed', 'after', 'treatment.', 'Winkler,', 'B.', 'C.,', 'Dunlap,', 'W.', 'J.,', 'Mizclle,', 'J.', 'W.,', 'Rohrbaugh,', 'L.', 'M.', 'and', 'Wender,', 'S.', 'H.', '83']