['SUBREPORT', 'P', '0500/3082', 'UBE152', '(S)', 'A2', 'AT', 'PAGE.', '1-1', '1', 'SUMMARY.', '1.1', 'Objective', 'and', 'Method', 'In', 'the', 'present', 'chronic', 'study', 'the', 'tumorigenic', 'activity', 'of', 'MAIN-', 'STREAM', 'WHOLE', 'SMOKE', 'CONDENSATE', 'collected', 'with', 'impaction', 'trap', '(MWSC-I),', 'of', 'the', 'standard', 'reference', 'cigarette', 'type', '2R1', 'was', 'deter-', 'mined', 'after', 'multiple', 'application', 'to', 'the', 'dorsal', 'skin', 'of', 'female', 'SENCAR', 'mice', 'for', '80', 'weeks.', 'MWSC-I', 'was', 'applied', 'without', 'and', 'with', 'pretreatment', 'with', 'a', 'single', 'dose', 'of', '7,12-dimethyl-benz(a)anthracene', '(DMBA).', 'For', 'this', 'purpose', '350', '(a)', 'SENCAR', 'mice', 'from', 'Battelle', 'Institute', '(CH,', 'Genf)', 'were', 'allocated', 'randomly', 'to', '2', 'control', 'and', '3', 'MWSC-I-', 'treated', 'groups', '(70', 'mice', 'per', 'group).', 'Each', 'group', 'had', '4', 'additional', 'mice', 'for', 'the', 'histological', 'evaluation', 'of', 'skin', 'irritations', '(b).', 'The', 'hair', 'of', 'the', 'application', 'area', 'was', 'sheared', 'with', 'electric', 'clippers', 'before', 'the', '1st', 'application', 'and', 'subsequently', 'at', 'least', '2', 'times/week.', 'At', 'the', 'age', 'of', '50', '+', '2', 'days', 'the', 'mice', 'of', '2', 'groups', 'received', 'a', 'single', 'dermal', 'application', 'of', '10:', 'nanomoles', 'DMBA', '(equivalent', 'to', '2.6', 'micro-', 'grams)', 'per', 'mouse.', 'At', 'this', 'dose', 'the', 'initiating', 'activity', 'of', 'DMBA', 'will', 'be', 'predominant', 'relative', 'to', 'the', 'promoting', 'activity.', '10', 'days', 'later', '(c)', 'MWSC-I', 'application', 'was', 'started.', 'Condensates', 'or', 'acetone', 'was', 'applied', 'with', 'automatic:', 'pipettes.', 'Doses', 'of', '60', 'and', '90', 'milli-', 'grams/(mouse', 'x', 'week)', 'of', 'dry', 'MWSC-I', 'prepared', 'in', 'acetone', '(d)', 'or', 'acetone', 'alone', 'were', 'applied', 'in', 'a', 'volume', 'of', '100', 'microliters/(mouse', 'x', 'application)', 'on', '5', 'consecutive', 'days', 'per', 'week.', ' ', '(a)', 'mice', 'for', 'cage', 'control', 'and', 'microbiological', 'analysis', 'not', 'in-', 'cluded', '(b)', 'sacrifice', 'shortly', 'after', 'the', 'maximal', 'irritative', 'response,', 'i.', 'e.', 'approx.', '3', 'weeks', 'after', 'the', 'start', 'of', 'MWSC-I', 'application', ')', 'no', 'applications', 'in', 'between', ')', 'Contrary', 'to', 'the', 'INBIFO', 'standard', 'procedure', 'used', 'with', 'conventio-', 'nal', 'mouse', 'strains,', 'nominal', 'doses', 'will', 'already', 'be', 'reached', 'after', '2', 'weeks', 'of', 'application.', 'During', 'weeks', '1', 'and', '2', 'the', 'doses', 'will', 'be', 'increased', 'daily', 'from', '10', 'to:', '100', 'percent', 'of', 'nominal', 'dose.', '(c', '(a', 'yISGEs606202']