['MAY21.90W', 'Page', '1', 'DRAFT-DRAFT-DRAFT-DRAFT-DRAFT-DRAFT-DRAFT-DRAFT-DRAFT-DRAFT~-DRAFT', 'HIGHLIGHTS', 'DATE:', 'May', '21,', '1990', 'BIOBEHAVIORAL', 'RESEARCH', 'Nicotine', 'Receptor', 'Pharmacology', 'Dr.', 'Fluhler', 'began', 'a', 'study', 'of', 'the', 'effects', 'of', 'glutamate', 'on', 'intracellular', 'calcium', 'concentrations', 'in', 'cultured', 'fetal', 'rat', 'cortical', 'neurons.', 'Initial', 'results', 'indicate', 'that', '3', 'uM', 'glutamate', 'causes', 'a', 'large,', 'rapid', 'increase', 'in', 'intracellular', 'calcium', 'that', 'does', 'not', 'return', 'to', 'starting', 'levels', 'for', 'at', 'least', '15', 'minutes.', 'Concentrations', 'of', 'glutamate', 'that', 'elicit', 'reversible', 'responses', 'will', 'be', 'sought.', 'If', 'an', 'adequate', 'dose-response', 'curve', 'for', 'glutamate', 'can', 'be', 'obtained,', 'the', 'effects', 'of', 'various', 'concentrations', 'of', 'nicotine', 'on', 'the', 'glutamate', 'response', 'will', 'be', 'determined.', 'New', 'system', 'software', 'for', 'the', 'Perceptics', 'Microscope', 'Workstation', 'was', 'installed.', 'Modification', 'of', 'image', 'acquisition', 'and', 'analysis', 'programs,', 'to', 'make', 'them', 'compatible', 'with', 'the', 'new', 'system', 'software', 'is', 'near', 'completion.', 'Several', 'new', 'programs', 'to', 'simplify', 'the', 'image', 'analysis', 'procedure', 'have', 'been', 'written.', 'Hoechst', 'Collaboration', 'Dr.', 'Lippiello', 'forwarded', 'the', 'following', 'to', 'Hoechst-Roussel:', 'a', 'statement', 'of', 'the', 'nicotinic', 'cholinergic', 'hypothesis', 'of', "Alzheimer's", 'disease', 'with', 'literature', 'summaries', 'supporting', 'the', 'hypothesis;', 'two', 'internal', 'reports', 'describing', 'the', 'Analog', 'Research', 'program', 'and', 'providing', 'available', 'data', 'on', 'specific', 'analogs;', 'several', 'reprints', 'of', 'RJRT', 'publications', 'in', 'the', 'nicotine', 'research', 'area.', 'Dr.', 'Richard', 'Effland,', 'the', 'point', 'person', 'for', 'Hoechst,', 'confirmed', 'that', 'he', 'had', 'received', 'the', 'materials.', 'Hoechst', 'scientists', 'will', 'now', 'review', 'the', 'information', 'and', 'their', 'medicinal', 'chemists', 'will', 'suggest', 'a', "set'", 'of', '5-7', 'compounds', 'to', 'be', 'included', 'in', 'the', 'joint', 'testing', 'program.', 'Smoke', 'Component', 'Dose', 'Analysis', 'of', 'plasma', 'and', 'saliva', 'samples', 'and', 'reduction', 'of', 'the', 'data', 'from', 'the', 'recent', 'pilot', 'study', 'are', 'almost', 'complete.', 'Results', 'for', 'nicotine', 'are', 'complete;', 'cotinine', 'results', 'should', 'be', 'complete', 'May', '22.', 'A', 'total', 'of', '51', 'samples', 'were', 'obtained', 'in', 'this', 'study.', 'A', 'total', 'of', '12', 'different', 'types', 'of', 'tea', 'were', 'examined', 'for', 'nicotine', 'content.', 'Thorough', 'water-extracts', 'of', 'the', 'teas,', 'as', 'well', 'as', 'beverage-quality', 'brews', 'of', 'each', 'were', 'prepared', 'for', 'analysis.', 'Both', 'decaffeinated', 'and', 'caffeine-containing', 'teas', 'were', 'included.', 'Mass', 'spectral', 'confirmation', 'of', 'the', 'identity', 'of', 'the', 'nicotine', 'GC', 'peak', 'will', 'be', 'provided', 'by', 'Dr.', 'Paul', 'Nelson', '(ETS', 'Division).', 'The', 'chromatographic', 'analysis', 'is', 'complete;', 'data', 'reduction', 'should', 'be', 'completed', 'by', 'May', '25.', 'Work', 'continues', 'on', 'the', 'new', 'HMSM.', 'Dr.', 'Griffith', 'and', 'Mr.', 'Bain', 'McConnell', '(PT&D)', 'redesigned', 'the', "module's", 'printed', 'circuit', 'board', 'into', 'two', 'boards', 'to', 'meet', 'space', 'constraints.', 'All', 'electronic', 'and', 'mechanical', 'designs', 'are', 'now', 'complete.', 'Software', 'development', 'is', 'underway', 'with', 'an', 'outside', 'contractor.', 'Dr.', 'Griffith', 'prepared', 'an', 'updated', 'timeline', 'for', 'the', 'HMSM', 'work', 'which', 'indicated', 'completion', 'of', 'the', '10-port', 'system', 'in', 'mid-September.', 'Progress', 'was', 'reviewed', 'for', 'the', 'Nicotine', 'RSM', 'Study', 'teanm.', 'Psychophysiology', 'of', 'Smoking', 'EEG', 'data', 'from', "Alzheimer's", 'patients', 'and', 'normal-elderly', 'subjects', 'were', 'supplied', 'by', 'Dr.', 'Kerry', 'Coburn', '(Southern', 'Illinois', 'School', 'of', 'Medicine).', 'These', 'data', 'were', 'treated', 'by', 'the', '"chaos"', 'analysis', 'program', 'at', 'the', 'Florida', 'State', 'University', 'supercomputer', 'center', 'and', 'returned', 'to', 'RIJRT', 'for', 'statistical', 'comparison.', 'Analysis', 'indicated', 'that,', 'compared', 'to', 'normal-young', 'subjects,', 'normal-elderly', 'subjects', 'showed', 'significant', 'loss', 'of', 'EEG', 'chaotic', 'complexity', 'over', 'the', 'front', 'half', 'of', 'the', '1882', '02ZS1S']