[' ', ' ', 'a)', ' ', 'J', '”', 'Reprinted', 'from:', 'ALCOHOL', 'AND', 'OPIATES', 'Neurochemical', 'and', 'Behavioral', 'Mechanisms', '©', '1977', ':', 'ACADEMIC', 'PRESS,', 'INC.', 'Vo', 'pat', 'NEW', 'YORK', '=', 'SAN', 'FRANCISCO.', '=—-', 'LONDON', 'ue,', 'B', 'Lie', 'aaah', '16.', 'ROLE', 'OF', 'PHOSPHATIDYL', 'SERINE', 'IN', 'THE', 'OPIATE', 'RECEPTOR*', 'F', '4', 'L.', 'G.', 'Abood,', 'F.', 'Takeda,', 'and', 'N.', 'Salem,', 'Jr.', '-', 'Center', 'for', 'Brain', 'Research', 'and', 'Department', 'of', 'Biochemistry,', 'University', 'of', 'Rochester', 'Medical', 'Center,', 'Rochester,', 'New', 'York,', '14642.', 'INTRODUCTION', 'The', 'molecular', 'nature', 'and', 'configuration', 'of', 'the', 'opiate', 'receptor', 'is', 'a', 'problem', 'under', 'intensive', 'investigation', 'in', 'a', 'number', 'of', 'laboratories', '(1,2,3)}.', 'Our', 'interest', 'in', 'the', 'problem', 'developed', 'as', 'a', 'result', 'of', 'a', 'finding', 'of', 'others', '(4)', 'that', 'a', 'proteolipid', 'fraction', 'from', 'brain', 'may', 'be', 'responsible', 'for', 'stereospecific', 'opiate', 'binding', 'observed', 'in', 'various', 'prepara-', '.tions', 'of', 'brain', 'tissue.', 'Upon', 'examining', 'various', 'lipids,', '~', 'proteolipids,', 'and', 'proteins', 'from', 'membranes', 'derived', 'from', 'brain,', 'it', 'was', 'found', 'that', 'phosphatidyl', 'serine', '(PS),+', 'the', 'major', 'acidic', 'lipid', 'in', 'brain,', 'exhibited', 'stereospecific', 'opiate', 'binding', '(5).', 'Since', 'the', "Ky's", 'for', 'the', 'opiate-PS', 'complex', 'and', 'the', 'opiate-', '_._', 'tissue', 'complex', 'differed', 'by', 'three', 'orders', 'of', 'magnitude,', 'it', 'was', 'recognized', 'that', 'the', 'binding', 'to', 'PS', 'was', 'nonspecific', 'and', 'dif-', 'ferent', 'from', 'the', 'high', 'affinity', 'binding', 'to', 'membrane', 'prepara-', 'tions.', 'What', 'interested', 'us', 'was', 'the', 'possibility', 'that', 'PS,', 'in', 'the', 'form', 'of', 'a', 'complex', 'with', 'a', 'membranous', 'protein,', 'may', 'be', 'an', 'important', 'component', 'of', 'the', 'opiate', 'receptor.', ';', 'In', 'an', 'effort', 'to', 'test', 'this', 'hypothesis', 'a', 'study', 'was', 'under-', 'taken', 'on', 'the', 'effect', 'of', 'added', 'phospholipids', 'on', 'opiate', 'binding.', 'It', 'had', 'been', 'known', 'for', 'many', 'years', 'that', 'exogenous', 'lipids', 'were', 'capable', 'of', 'exchanging', 'with', 'endogenous', 'ones', 'with', 'no', 'apparent', 'alteration', 'in', 'their', 'natural', 'functional', 'or', 'structural', ' ', '*', 'This', 'research', 'was', 'supported', 'by', 'USPH', 'grant', 'DAQ0464.', 't+', 'The', 'following', 'abbreviations', 'are', 'used:', 'PS', '=', 'phosphatidyl', '\\', 'serine,', 'PC', '=', 'phosphatidyl', 'choline,', 'PE', '=', 'phosphatidyl', 'ethanolamine,', 'PA', '=', 'phosphatidic', 'acid,', 'PI', '=', 'phosphoinosi-', 'tides,', 'CTAB', '=', 'cetyltrimethylammonium', 'bromide,', 'CPC', '=', 'cetylpyridinium', 'chloride,', 'FSC', '=', 'cationic', '"Zonyl"', 'fluoro-', 'surfactant', '(DuPont),', 'SDS', '=', 'sodium', 'dodecylsulfate,', 'DNDFB', 'dinitrodifluorobenzene,', '7H-DHM', '=', '34-dihydromorphine.', '283', 'G908ZTO00']