['Page', '11', 'The', 'analytical', 'methods', 'used', 'to', 'determine', 'TPM,', 'CO,', 'nicotine,', 'nitrogen', 'oxides,', 'aldehydes,', 'ammonia,', 'and', 'the', 'particle', 'size', 'distribution', 'as', 'well', 'as', 'those', 'for', 'temperature', 'and', 'relative', 'humidity', 'in', 'the', 'exposure', 'chambers', 'were', 'performed', 'as', 'previously', 'described', '(Haussmann', 'et', 'al.,', '1998).', 'The', 'other', 'analytes', 'were', 'determined', 'as', 'follows:', '3-Ethenyl-pyridine', 'and', 'nicotine', 'were', 'determined', 'in', 'parallel', 'using', '2-ethenyl-pyridine', 'as', 'internal', 'standard.', 'Solaneso!l', 'was', 'determined', 'in', 'particulate', 'matter', 'after', 'trapping', 'on', 'Fluoropore', 'membrane', 'filters', '(pore', 'size:', '1', 'pm;', 'Millipore,', 'Eschborn,', 'Germany)', 'by', 'reversed', 'phase', 'HPLC', '(Lichraspher', 'RP-select', 'B,', '5', 'um,', '125', 'x', '3', 'mm,', 'Merck,', 'Darmstadt,', 'Germany)', 'and', 'UV', 'detection', '(HP', '1090,', 'Hewlett', 'Packard,', 'Waldbronn,', 'Germany).', 'Isoprene,', 'toluene,', '1,3-butadiene,', 'and', 'benzene', 'were', 'trapped', 'in', 'methanol', 'at', '-782C', 'and', 'determined', 'by', 'gas', 'chromatography/mass', 'spectrometry', '(GC/MS;', 'Hewlett', 'Packard', '5890A/5970B)', 'with', 'a', 'DB-5.625', 'column', '(30', 'm', 'x', '0.25', 'mm,', 'J', 'and', 'W,', 'Fisons,', 'Wiesbaden,', 'Germany).', 'Phenols', 'were', 'determined', 'in', 'the', 'particulate', 'phase', 'after', 'trapping', 'on', 'Cambridge', 'type', 'glass', 'fiber', 'filters', '(Gelman,', 'Ann', 'Arbor,', 'MI,', 'USA),', 'extraction,', 'and', 'silylation', 'using', 'GC/MS', 'with', 'a', 'DB-5.625', 'column.', 'Polycyclic', 'aromatic', 'hydrocarbons', 'were', 'extracted', 'from', 'a', 'TPM-loaded', 'glass', 'fiber', 'filter', 'with', 'methanol/water', 'followed', 'by', 'back', 'extraction', 'with', 'hexane,', 'clean-up', 'by', 'solid', 'phase', 'extraction', '(Bakerbond', 'amino,', 'Baker,', 'Gross-Gerau,', 'Germany),', 'and', 'analyzed', 'by', 'GC/MS', 'with', 'a', 'DB-17', 'column', '(30', 'm', 'x', '0.25', 'mm,', 'J', 'and', 'W).', 'N-nitrosamines', 'were', 'trapped', 'in', 'citrate/phosphate', 'buffer', 'with', 'ascorbic', 'acid', 'and', 'on', 'glass', 'fiber', 'filters', 'connected', 'in', 'series.', 'The', 'combined', 'dichloromethane', 'extracts', 'were', 'washed', 'with', 'a', 'sodium', 'hydroxide', 'solution', 'and', 'cleaned', 'by', 'adsorption', 'chromatography', 'on', 'aluminum', 'oxide.', 'The', 'N-nitrosamines', 'were', 'determined', 'by', 'GC', 'with', 'a', 'DB-5', 'column', '30', 'm', 'x', '0.53', 'mm', '(ICT,', 'Bad', 'Homburg,', 'Germany)', 'and', 'a', 'thermal', 'energy', 'analyzer', '(TEA', '543,', 'Thermo', 'Electron', 'Corporation,', 'via', 'Isconlab,', 'Heidelberg,', 'Germany).', 'For', 'the', 'determination', 'of', 'the', 'metals,', 'SS', 'was', 'collected', 'on', 'membrane', 'filters', 'with', '0.22', 'um', 'pore', 'size', '(GSWP', '02500,', 'Millipore).', 'After', 'the', 'filters', 'were', 'digested', 'with', 'pOOrE99L0e', 'mere']