['Respiratory', 'tests', 'were', 'performed', 'to', 'measure', 'respiratory', 'rate,', 'tidal', 'volume,', 'and', 'minute', 'volume.', 'These', 'measurements', 'were', 'taken', 'during', 'a', 'control', 'period', 'and', 'five', 'minutes', 'after', 'the', 'administration', 'of', 'each', 'dosage.level', 'of', 'the', 'test', 'compound', 'as', 'well', 'as', 'the', 'compound', 'vehicle.', 'It', 'is', 'expected', 'that', 'these', 'functional', 'tests', 'will', 'provide', 'a', 'valid', 'basis', 'for', 'an', 'assessment', 'of', 'the', "compound's", 'safety', 'in', 'human', 'use.', 'It', 'is', 'generally', 'accepted', 'that', 'the', 'results', 'of', 'a', 'pharmacodynamic', 'analysis', 'of', 'compound', 'activity', 'on', 'the', 'cardiovascular', 'system', 'of', 'the', 'above', 'model', 'correlates', 'well', 'with', 'human', 'responses,', 'especially', 'with', 'respect', 'to', 'potential', 'adverse', 'reactions.', 'Test', 'Article', 'The', 'tast', 'article,', 'designated', 'as', 'B-85,', 'was', 'received', 'from', 'the', 'sponsor', 'on', 'July', '13,1981.', 'It', 'was', 'stored', 'in', 'the', 'glass', 'vial', 'in', 'which', 'it', 'was', 'received,', 'at', 'room', 'temperature.', 'A1l', 'data', 'which', 'appropriately', 'characterize', 'the', 'test', 'article', 'with', 'respect', 'to', 'identity,', 'strength,', 'purity', 'composition,', 'and', 'stability', 'under', 'conditions', 'of', 'use', 'are', 'retained', 'by', 'the', 'sponsor.', 'Animal', 'and', 'Husbandry', 'Fifteen', 'month', 'old', 'purebred', 'Beagle', 'dogs', 'were', 'received', 'from', 'Theracon,', 'Inc.,', 'Topeka,', 'Kansas', 'on', 'October', '27,', '1981.', 'Quarantine', 'The', 'dogs', 'were', 'immunized', 'against', 'distemper,', 'hepatitis,', 'and', 'leptospirosis,', 'and', 'were', 'maintained', 'in', 'quarantine', 'for', 'a', 'minimum', 'of', 'two', 'weeks.', 'Animals', 'were', 'observed', 'daily', 'for', 'general', 'physical', 'appearance', 'and', 'behavior.', 'Assignment', 'to', 'Groups', 'Three', 'dogs', 'in', 'good', 'health', 'of', 'the', 'same', 'sex', 'were', 'randomly', 'selected', 'from', 'the', 'quarantine', 'population.', 'Prepération', 'and', 'Administration', 'of', 'the', 'Test', 'Article', 'On', 'the', 'day', 'of', 'cardiac', 'testing', 'appropriate', 'amounts', 'of', 'the', 'test', 'article', 'were', 'mixed', 'with', '0.9%', 'normal', 'physiological', 'saline', 'to', 'yield', 'a', 'final', 'concentration', 'of', '2', 'mg/ml.', 'The', 'test', 'material', 'was', 'administered', 'i.v.', 'as', 'this', 'is', 'the', 'accepted', 'standard', 'for', 'cardiovascular', 'tests.', 'Dosages', 'were', 'calculated', 'based', 'on', 'the', 'body', 'weight', 'of', 'the', 'animal.', 'BLI', '#1416(8)', '-2-', 'Secagrgg']