['ABSTRACTS', 'Mutation', 'Research', 'Vol.', '241,', '1990,', 'p.', '37', 'A', 'COMPARISON', 'OF', 'DNA', 'ADDUCT', 'FORMATION', 'IN', 'WHITE', 'BLOOD', 'CELLS', 'AND', 'INTERNAL', 'ORGANS', 'OF', 'MICE', 'EXPOSED:', 'TO', 'BEN20O[', 'a]PYRENE,', 'DIBENZO{c,qg]', 'CARBAZOLE,', 'SAFROLE', 'AND', 'CONDENSATE', 'Measurement', 'of', 'tissue/cell', 'DNA:', 'adducts', 'represents', 'a', 'suitable', 'monitor', 'of', 'carcinogen', 'exposure', 'because', 'the', 'majority', 'of', 'chemical', 'mutagens,/', 'carcinogens', 'react', 'with', 'DNA,', 'forming', 'covalent', 'adducts;', 'a', 'key', 'event', 'in', 'the', 'initiation', 'of', 'chemical', 'carcinogenesis..', 'Investigations', 'of', 'DNA-adduct', 'formation', 'in', 'vivo', 'in', 'white', 'blood', 'cells', '(WBC)', 'versus', 'target', 'tissues,', 'i.e.', 'internal', 'organs.', 'for.', 'most', 'carcinogens,', 'is', 'expected', 'to', 'yield', 'useful', 'information', 'about', 'the', 'suitability', 'of', 'WBC', 'for', 'biomonitoring', 'and', 'risk', 'assessment.', 'For', 'this', 'purpose,', 'female', 'ICR', 'mice', 'were', 'given', '0.4', 'mamole', '/kg', 'benzof', 'alpytene', '(BP),', '0:045', 'mmole/kg', 'dibenzofc;', 'glcarbazole', '(DBC)', 'or', '2.47', 'mmole', 'kg', 'safrole', 'by', 'oral', 'gavage', 'or', '4', 'daily', 'doses', '(equivalent', 'to', '3', 'cigarettes)', 'of', 'cigarette-smoke', 'condensate', '(CSC)', 'by', 'topical', 'application.', 'At', '24', 'L', 'after', 'dosing,', 'DNA', 'adducts', 'were', 'detected', 'by', 'anuclease', 'Pl-enhanced', 'nP‘-posllhbeling', 'assay', '[M.V.', 'Reddy', 'and', 'K.', 'Randerath,', 'Carcinogenesis,', '7', '(1986)', '1543]', 'in', 'WBC', 'and', 'intemal', 'tissues', 'treated.', 'with', 'individual', 'carcinogens;', 'while', "CSC'", 'treatment.', 'elicited', 'aromatic', 'adducts', 'in', 'most', 'tissues', 'but', 'not', 'in', 'WBC.', 'Adduct', 'patterns', 'of', 'WBC', 'DNA', 'were', 'qualitatively', 'similar', 'to', 'those', 'internal', 'organs,', 'but', 'adduct', 'amounts', 'varied.', 'B,', 'a', 'systemic', 'carcinogen;', 'bound', 'nearly', 'as', 'muck', 'to', 'WBC', 'DNA', 'as:', '1o', 'target-tissue', 'DNA:', 'samples;', 'whereas:', 'the', 'liver', 'carcinogens,', 'DBC', 'and', 'safrole,', 'bound', '1o', 'WBC', 'DNA', 'considerably', 'less', '(22-', 'and', '51-fold,', 'respectively)', 'compared', 'with', 'liver', 'DNA.', 'The', 'number', 'of', 'adducts', 'in', '107', 'nucleotides', 'of', 'WBC,', 'liver,', 'lung,', 'kidney', 'and', 'spleen.', 'DNA,', 'respectively,', 'were:', '2,.5;', '3,', '2', 'and', '3', 'with', '6,', '14', 'and', '4', 'with', 'DBC;', '5,', '238;', '3,', '5', 'and', '0.6', 'with', 'safrole.', 'For', 'CSC,', 'these', 'values', 'were', '0,', '1.and', '0.02', 'in', 'WBC,', 'lung', 'and', 'spleen,', 'respectively.', 'Our', 'results', 'show.', 'that', 'carcinogen', 'binding', 'to:', 'WBC', 'DNA', 'does', 'not', 'reflect', 'binding', 'to', 'target-tissue', 'DNA,', 'in', 'aiquantitative', 'sense', 'for', 'the', 'carcinogens', 'studied', 'except', 'for', 'BP,', 'that', 'WBC', 'are', 'not', 'suitable', 'surrogates', 'for', 'monitoring', 'CSC', 'exposure', 'by', 'DNA-adduct.', 'measurementi', 'after', 'topical', 'application.', 'The', 'CSC', 'data', 'in', 'mice', 'was', 'consistent', 'with', 'the', 'previous', 'findings', 'in.', 'humans', 'thati', 'smokers’', 'tissues.', 'but', 'not', 'WBC', 'show,_smoking-related', 'bulky/aromatic', 'DNA', 'adducts,', 'as', 'measured.by.', 'SZETIESS', '399']