['Draft', 'Report', '“«', '1-2486.005', 'VI.', 'METHODS', 'Animais:', 'Female', 'BSC3F1', 'mice:', 'were', 'obtained', 'from', 'Charles:', 'River', 'Breeding', 'Laboratories,', 'Kingston,', 'New', 'York', 'at', '3', 'weeks', 'of', 'age.', 'Upon', 'arrival,', 'sentinel.', 'animals', 'were', 'checked', 'for', 'parasites', 'and', 'serum', 'checked', 'for', 'titers', 'to', 'adventitious', 'agents', '(Reovirus', 'type', '3,', 'Pneumonia', 'virus', 'of', 'mice,', 'K', 'virus,', 'Encephalomyelitis', 'virus,', 'Polyoma', 'virus,', 'Sendai', 'virus,', 'Minute', 'virus', 'of', 'mice,', 'Mouse', 'adenovirus,', 'Mouse', 'Hepatitis', 'virus,', 'Lymphocytic', 'choriomeningitis:', 'virus,', 'Ectromelia', 'virus,', 'and', 'Mycoplasma', 'pulmonis).', 'Cultures.', 'from', 'the', 'oropharyngeal.', 'area', 'of', 'the', 'respiratory', 'tract', 'were', 'examined', 'for', 'the', 'presence', 'ofi', 'pathogens.', 'All', 'the', "animal's", 'were', 'vaccinated', 'against.', 'Sendai:', 'virus', 'and', 'quarantined', 'for', '28', 'days.', 'Stringent', 'disease', 'control', 'procedures', 'were', 'fallawed', 'during', 'quarantine', 'to', 'assure', 'the', 'use', 'of', 'healthy', 'animals.', 'The', 'animals', 'were', 'judged', 'to', 'be', 'healthy', 'prior', 'to', 'utilization', 'in', 'this', 'study', 'and', 'were', '9', 'weeks', 'old', 'at', 'initiation', 'of', 'expasure..', 'Animals.', 'were', 'housed:', 'in', 'an', 'AAALAC-accredited', 'facility', 'with', 'a', 'controlted', 'environment', 'and', 'a.', '12', 'hour', 'light/dark', 'cycle.', 'While', 'on', 'test,', 'the', 'temperature', 'and', 'humidity', 'varied', 'between', '72', '+', '2°', 'F', 'and', '30', '-', '96%', 'relative', 'humidity.', 'Mice', 'were', 'housed', '4', 'per', 'cage', 'in', 'polycarbonate', 'autoclavable', 'cages', 'with', 'filter', 'tap', 'cage', 'lids.', 'Corn-cob', 'bedding', 'was', 'used', 'and', 'animals', 'had', 'free', 'access', 'to', 'certified', 'laboratory', 'rodent.', 'chow', 'which', 'had', 'been', 'analyzed!', 'for', 'environmental', 'contaminants.', 'Water', 'and', 'food', 'were', 'provided', 'ad', 'libitum,', 'except', 'during', 'exposures', 'an', 'the', 'smoking', 'machines:', 'Smoke', 'Generation', 'and', 'Exposures:', 'Cigarettes', 'were', 'conditioned', 'at', 'room', 'temperature', 'and', '40', '+', '10%', 'relative', 'humidity', 'for', 'at', 'least:', '24', 'hours', 'prion', 'to', 'use.', 'Fresh,', 'whole', 'cigarette', 'saoke', 'was', 'generated', 'ty', 'means', 'of', 'a', 'Walton', 'Horizontal', 'Smoking', 'Machine', '(Walton;', 'Process', 'and:', 'Instruments,', 'Corp.,', 'Brooklyn,', 'NY}.', 'The', 'operating', 'principles', 'and', 'use', 'af', 'this', 'machine', 'with', 'mice', 'have', 'been', 'described', '(Guerin', 'et', 'al.,', '9793', 'Henry', 'et', "al'.,,", '1980).', 'The', 'Walton', 'generates', 'smoke', 'under', 'the', 'standard', 'conditions', 'of', 'a', '2', 'second', 'puff,', '35', 'ml', 'puff', 'vollume,', 'one', 'puff', 'per', 'minute.', 'The', 'smoke', 'is', 'held', 'in', 'the', 'chamber', 'for', 'an', 'additional', '28.', 'seconds,', 'then', 'the', 'chamber', 'is', 'purged', 'for', '30', 'seconds', 'with', 'fresh', 'air.', 'The', 'cycle', 'is', 'then', 'repeated', 'for', 'the', 'designated', 'number', 'of', 'puffs', 'per', 'cigarette.', 'A', 'rest', 'period', 'of', 'air', '(8', 'minutes)', 'was.', 'given', 'between', 'each', 'smoke', 'exposure.', 'Z29ILOES']