['4.', '4,', 'LABORATORY', 'SPACE', 'and', 'FACILITIES', 'available', 'to', 'the', 'applicant.', 'State', 'location', 'if', 'facilities', 'are', 'elsewhere', 'than', 'the', "applicant's", 'institution.', 'Indicate', 'any', 'facilities', 'that', 'are', 'required', 'but', 'are', 'not', 'currently', 'available.', 'The', 'proposed', 'studies', 'will', 'be', 'performed', 'in', 'laboratory', 'space', 'assigned', 'to', 'the', 'investigator', 'through', 'the', 'Department', 'of', 'Molecular', 'Microbiology', 'and', 'Immunology', 'at', 'the', 'University', 'of', 'Missouri-Columbia.', 'This', 'includes', 'a', 'general', 'laboratory', 'of', 'modern', 'design', '(900', 'ft®)', 'equipped', 'for', 'specialized', 'procedures', '(e.g.,', 'isotopes,', 'fume', 'hoods),', 'and', 'a', 'separate,', 'isolated', '250', 'ft?', 'tissue', 'culture', 'facility.', 'This', 'space', 'is', 'located', 'on', 'the', 'sixth', 'floor', 'of', 'the', 'Health', 'Sciences', 'Center', 'at', 'UMC,', 'and', 'is', 'adjacent', 'to', 'other', 'laboratories', 'within', 'the', 'Department', 'as', 'well', 'as', 'other', 'common', 'facilities', '(e.g.,', 'cold', 'rooms,', 'fluorescent', 'microscope,', 'dishwashing,', 'autoclaves,', 'etc.}.', 'Dr.', 'Pintel', 'has', 'office', 'space', 'located', 'near', 'the', 'laboratory', 'space', 'listed', 'above.', 'An', 'excellent', 'and', 'inexpensive', 'DNA', 'core', 'facility,', 'Protein', 'Core', 'facility,', 'Cell', 'and', 'Immunology', 'core', 'facility', 'are', 'available', 'for', 'use.', 'All', 'the', 'major', 'equipment', 'items', 'required', 'for', 'the', 'proposed', 'studies', 'are', 'available', 'to', 'the', 'investigator', 'within', 'his', 'faboratory', 'or', 'within', 'the', 'Department.', 'Additional', '-70', 'freezer', 'space', 'is', 'required,', 'however.', 'Secretarial', 'support', 'services,', 'photocopying,', 'and', 'word', 'processing/graphics', 'capabilities', 'are', 'available', 'to', 'the', 'investigators', 'through', 'the', 'Department', 'of', 'Molecular', 'Microbiology', 'and', 'Immunology.', 'There', 'are', 'also', 'machine', 'shop,', 'instrument', 'shop', 'and', 'glass', 'blowing', 'facilities', 'on', 'campus.', 'Photography', 'and', 'artists', 'services', 'are', 'within', 'the', 'Medical', 'Schcol.', '5.', 'BUDGET', 'JUSTIFICATION', '-', 'Use', 'this', 'space', 'to', 'explain', 'specific', 'needs', 'for', 'items', 'described', 'on', 'budget', 'pages.', 'Personnel:', 'David', 'J.', 'Pintel:', 'Dr.', 'Pintel', 'is', 'the', 'principal', 'investigator', 'for', 'this', 'project.', 'He', 'will', 'be', 'responsible', 'for', 'the', 'scientific', 'direction', 'of', 'the', 'project,', 'the', 'day-to-day', 'progress', 'of', 'the', 'proposed', 'work,', 'organization,', 'preparation,', 'and', 'submission', 'of', 'manuscripts.', 'Funds', 'are', 'requested', 'to', 'support', '15%', 'effort.', 'Anja', 'Maria', 'Séderlund:', 'Dr.', 'Sdéderlund', 'was', 'chosen', 'over', 'a', 'number', 'of', 'other', 'candidates', 'based', 'on', 'her', 'strong', 'background', 'in', 'parvovirology,', 'excellent', 'recommendations,', 'and', 'her', 'excellent', 'scientific', '"fit"', 'to', 'this', 'lab.', 'She', 'plans', 'to', 'join', 'the', 'lab', 'on', '4/96', 'with', 'extensive', 'experience', 'in', 'molecular', 'biology,', 'RNA', 'characterization,', 'the', 'preparation', 'and', 'screening', 'and', 'handling', 'of', 'genomic', 'libraries,', 'and', 'protein', 'purification.', 'She', 'will', 'spend', '100%', 'of', 'her', 'time', 'on', 'the', 'projects', 'proposed.', 'Amy', 'Riesselman:', 'Amy', 'is', 'a', 'talented', 'researcher', 'who', 'has', 'already', 'gained', 'considerable', 'experience', 'working', 'in', 'this', 'system,', 'and', 'will', 'be', 'invaluable', 'to', 'its', 'timely', 'progress.', 'She', 'will', 'devote', '50%', 'of', 'her', 'effort', 'to', 'this', 'project.', 'Equipment:', 'We', 'currently', 'have', 'one', '12', 'year', 'old', '—', '70°C', 'freezer', 'that', 'is', 'completely', 'full.', 'Significant', '—', '70°', 'capacity', 'is', 'required', 'for', 'the', 'proposed', 'project.', 'Supplies:', 'The', 'supply', 'budget', 'is', 'based', 'on', 'a', 'careful', 'assessment', 'of', 'the', 'costs', 'incurred', 'by', 'my', 'laboratory', 'over', 'the', 'last', '10', 'years.', 'We', 'will', 'have', 'a', 'particularly', 'high', 'use', 'of', 'enzymes,', 'oligonucleotides', 'and', 'radionucleotides', 'for', 'this', 'project.', 'Other:', 'Funds', 'are', 'requested', 'for', 'publication', 'costs', '(fees,', 'artist', 'and', 'photography', 'services),', 'LN,', 'CO,,', 'and', 'my', 'share', 'of', 'service', 'contracts', 'on', 'two', 'Departmental', 'ultracentrifuges', 'and', 'one', 'high', 'speed', 'centrifuge.', '6.', 'APPENDIX:', 'Place', 'the', 'appendix', 'materials', 'after', 'the', 'original', 'and', 'each', 'copy', 'of', 'the', 'application', 'form', 'as', 'indicated', 'in', 'the', 'Instructions', 'for', 'New', 'Applications.', 'a.', 'Biographical', 'Sketches', 'of', 'the', 'professional', 'personnel', 'to', 'be', 'associated', 'with', 'the', 'project.', 'Each', 'sketch', 'should', 'be', 'NO', 'MORE', 'THAN', 'TWO', '(2)', 'PAGES.', 'The', 'NIH', 'format', 'is', 'acceptable.', 'The', 'P.I.', 'should', 'include', 'and', 'indicate', 'by', 'an', 'asterisk', 'the', 'FIVE', '(5)', 'most', 'significant', 'publications', 'whether', 'or', 'not', 'they', 'relate', 'directly', 'to', 'this', 'application.', 'b.', 'Supporting', 'material', '(such', 'as', 'letters', 'of', 'coliaboration).', 'c.', 'Copies', 'of', 'not', 'more', 'than', 'FIVE', '(5)', 'of', 'the', "applicant's", 'publications', 'or', 'manuscripts', 'that', 'are', 'pertinent', 'to', 'the', 'project.', '7.', 'ABSTRACTS', 'of', 'PUBLICATIONS', ':', 'Only', 'one', 'set', 'is', 'required.', 'See', 'Instructions', 'for', 'New', 'Applications.', 'Submit', 'ONE', 'PHOTOCOPY', 'of', 'the', 'abstract', 'page', 'of', 'each', '"pertinent', 'publication"', 'included', 'in', 'the', 'appendix', '(6.c.)', 'above;', 'For', 'each', 'manuscript,', 'submit', 'a', 'single', 'composite', 'page', 'that', 'includes', 'authors,', 'title,', 'journal,', 'abstract', 'and', 'publication', 'status', '(for', 'example,', '"submitted', 'for', 'publication").', 'NA-APPL.DOC', '8/13/93', 'CONFIDENTIAL', ' ', '50697427']