['262', 'As', 'with', 'the', 'other', 'subchronic', 'studies,', 'endpoints', 'of', 'body-weight', 'change,', 'feed', 'consumption,', 'gross', 'pathology,', 'organ', 'weights,', 'hematology,', 'clinical', 'chemistry', 'and', 'histopathology', 'were', 'used.', 'Special', 'endpoints', 'consisted', 'of', 'blood', 'COHb,', 'plasma', 'nicotine,', 'minute', 'ventilation', 'and', 'special', 'stains', 'for', 'respiratory', 'tissues.', '8.12.3.', 'Inhalation', 'Exposures', 'A', 'commercially', 'available', 'smoke', 'inhalation', 'device', '(AMESA,', 'Geneva)', 'previously', 'described', 'in', 'Section', '8.2.4.1.', 'was', 'used', 'for', 'these', 'studies.', 'Seven', 'puffs', 'were', 'taken', 'from', 'each', 'cigarette.', 'An', 'independent', 'exposure', 'device', 'was', 'used', 'with', 'each', 'type', 'of', 'cigarette', 'and', 'the', 'sham-exposed', 'animals.', 'The', 'animal', 'restraint', 'tubes', 'were', 'modified', 'to', 'house', 'hamsters.', '8.12.4.', 'Smoke', 'Composition', 'In', 'all', 'cases,', 'TPM', 'concentrations', 'were', 'close', 'to', 'the', 'targets.', 'In', 'the', 'TM-5', 'groups,', 'the', 'overall', 'mean', 'TPM', 'concentrations', 'were', '0.13,', '0.35', 'and', '0.64', 'mg/liter;', 'in', 'the', 'reference', 'groups,', 'the', 'concentrations', 'were', '0.12,', '0.35', 'and', '0.65', 'ng/liter.', 'These', 'TPM', 'concentrations', 'resulted', 'in', 'different', 'nicotine', 'deliveries', 'in', 'the', 'groups:', '3.2,', '8.1', 'and', '16.6', 'ug/liter', 'in', 'the', 'TM-5', 'groups;', 'and', '7.2,', '19.5', 'and', '39.2', 'in', 'the', 'reference', 'groups.', 'The', 'co', 'deliveries', 'to', 'the', 'hamsters', 'were', 'relatively', 'close:', '103,', '319', 'and', '662', 'ppm', 'in', 'the', 'TM-5', 'groups;', 'and', '141,', '435', 'and', '860', 'ppm', 'in', 'the', 'reference', 'groups.', 'The', 'particle', 'size', 'distributions', 'were', 'similar', 'in', 'the', 'aifferent', 'groups:', 'the', 'MMAD', 'values', 'were', 'around', '0.65', 'um;', 'and', 'the', 'sigma', 'g', 'values', 'were', 'around', '1.45.', '8.12.5.', 'Animal', 'Model', 'and', 'Animal', 'Husbandry', 'Male', 'and', 'female', 'Golden-Syrian', 'hamsters', 'six', 'to', 'eight', 'weeks', 'of', 'age', 'were', 'obtained', 'from', 'Harland', 'Sprague-Dawley,', '(Indianopolis,', 'IN).', 'Animal', 'husbandry', 'was', 'essentially', 'as', 'described', 'in', 'Section', '8.2.5.', 'except', 'that', 'the', 'hamsters', 'were', 'maintained', 'in', 'stainless', 'steel,', 'hanging', 'cages.', '8.12.6.', 'Protocol', 'Deviations', 'There', 'were', 'no', 'protocol', 'deviations', 'that', 'would', 'affect', 'the', 'integrity', 'of', 'the', 'study.', '8.12.7.', 'In-Life', 'Data', '8.12.7.1.', 'Serology', 'Serology', 'was', 'negative', 'throughout', 'the', 'study,', 'indicating', 'the', 'animals', 'were', 'not', 'compromised', 'by', 'infection.', '8.12.7.2.', 'Body', 'Weight', 'TPET', 'BCSTsS']