['22/08', '96', '16:42', 'ris', '2203', 'Soase2', '—INBIFO', 'KoeIn', '42+', 'GNI', 'PGR', '\\loo4/o10', '{NBIFO', 'institut', 'flr', 'biologische', 'Forschung', 'GmbH', '«', 'KdIn', 'PREQRITY', 'Intermediate', 'Report', 'P', '0500/3261', '22/8/96', 'Page', '3', ' ', 'triplicate', '(replicate', 'plating)', 'at', 'the', 'doses', '0.04,', '0.08,', '0.12,', 'and', '0.16', 'mg', 'dry', 'condensate/plate', 'using', 'an', 'application', 'volume', 'of', '50', 'sul/ptate,', '3', 'Experimental', 'Conditions', 'The', 'mutagenicity', 'assay', 'was', 'carried', 'out', 'in', 'basic', 'conformity', 'with', 'OECD', 'guideline', 'no.', '471', '(1983)', '*', 'with', 'a', 'reduced', 'protocol', 'using', '2', 'tester', 'strains,', 'S9', 'metabolization,', '4', 'test', 'substance', 'doses,', 'and', '1', 'mutagenicity', 'determination.', 'The', 'Salmonella', 'typhimurium', 'strains', 'were', 'confirmed', 'to', 'be', 'in', 'accordance', 'with', 'the', 'published', 'and/or', 'historical', 'data', 'on', 'thelr', 'genotypes,', 'number', 'of', 'spontaneous', 'revertants,', 'and', 'response', 'to', 'diagnostic', 'mutagens.', 'For', 'the', 'metabolic', 'promutagen', 'activation', 'of', 'the', 'test', 'substance,', 'a', 'postmitochondrial', '(S89)', 'fraction', 'from', 'the', 'livers', 'of', 'rats', 'treated', 'with', 'Arocior', '1254', 'was', 'used.', 'The', 'S93', 'amount', 'was', '1', 'mg', 'SS', 'protein', 'per', 'plate.', 'The', 'specific', 'mutagenicity', 'of', 'the', 'test', 'substance', 'was', 'calculated', 'from', 'the', 'approximately', 'linear', 'dose-response', 'curve', 'expressed', 'as', 'the', 'increase', 'in', 'the', 'number', 'of', 'revertants', 'per', 'mg', 'dry', 'condensate', 'using', 'weighted', 'linear', 'regression', 'analysis', 'with', 'Poisson', 'weights.', 'The', 'statistical', 'comparison', 'between', 'the', 'specific', 'mutagenicity', 'values', 'of', 'the', 'research', 'cigarettes', 'was', 'performed', 'using', 'the', 't-test', '(modified', 'according', 'to', 'Welch', '(Sachs,', '1982)', '°', 'in', 'case', 'of', 'heterogeneous', 'variances),', 'The', 'mutagenicity', 'of', 'the', 'MSC', 'of', 'the', 'standard', 'reference', 'cigarette', '1R4F,', 'smoked', 'and', 'assayed', 'in', 'parallel', 'as', 'an', 'internal', 'control,', 'was', 'in', 'accordance', 'with', 'previous', 'INBIFO', 'data.', '“', 'OECD', 'Guideline', '471,', 'Salmonella', 'typhimurium,', 'reverse', 'mutation', 'assay,', 'in:', 'OECD', 'guidelines', 'for', 'testing', 'of', 'chemicals,', 'pened', 'Organization', 'for', 'Economic', 'Co-operation', 'and', 'Development,', '®', 'Sachs,', 'L:', 'Applied', 'statistics,', 'New', 'York:', 'Springer', 'Verlag,', '1982', 'LZ08Z8902']