[' ', 'retention,', 'and', 'some', 'of', 'the', 'applications', 'thereof,', 'may', 'be', 'found', 'in', 'several', 'pub1i-', 'cations', '(Essman', '§', 'Alpern,', '1965;', 'Essman,', '1968;', 'Essman', 'and', 'Essman,', '1969).', 'Animals', 'utilized', 'in', 'all', 'of', 'the', 'proposed', 'studies', 'will', 'be', 'CF-1', 'strain', 'mice,', '(Mus', 'Musculus),', 'to', 'be', 'obtained', 'from', 'a', 'commercial', 'vendor', '(Carworth', 'Farms,', 'Inc.,', 'New', 'City,', 'New', 'York),', 'at', 'weaning', '(21', 'days)', 'and', 'following', 'adaptation', 'to', 'conditions', 'of', 'laboratory', 'use', '(10/cage),', 'will', 'be', 'utilized', 'as', 'experimental', 'subjects', 'at', 'approxi-', 'mately', '30', 'days', 'of', 'age', 'or,', 'in', 'the', 'case', 'of', 'the', 'developmental', 'studies', 'proposed,', 'animals', 'will', 'be', 'bred', 'in', 'the', 'laboratory', 'specifically', 'for', 'purposes', 'of', 'obtaining', 'precise', 'age', 'controls.', 'The', 'specific', 'experiments', 'to', 'be', 'conducted', 'within', 'the', 'time', 'specified', 'for', 'the', 'proposed', 'research', 'will', 'concern:', '(1)', 'the', 'effects', 'of', 'nicotine', 'and', 'its', 'active', 'metabolites', 'upon', 'memory', 'consolidation', 'during', 'early', 'development', ';', '(2)', 'identification', 'of', 'the', 'regional', 'uptake', 'and', 'distribution', 'of', 'nicotine', 'metabolites', 'in', 'the', 'brain', 'following', 'administration;', '(3)', 'the', 'temporal', 'relationship', 'between', 'central', 'uptake', 'of', 'nicotine', 'and', 'its', 'metabolites', 'and', 'the', 'distribution', 'and', 'alterations', 'in', 'biogenic', 'amines', ';', '(3)', 'specific', 'cellular', 'effects', 'of', 'nicotine', 'and', 'its', 'metabolites', 'as', 'related', 'to', 'memory', 'consolidation;', '9.', 'Physical', 'Facilities', 'Available:', 'Two', 'large', 'air-conditioned', 'laboratories', 'and', 'adjoining', 'animal', 'housing', 'facilities', 'ara', 'available', 'and', 'currently', 'in', 'use', 'by', 'the', 'investigator;', 'the', 'laborato-', 'ries', 'are', 'completely', 'equipped', 'with', 'all', 'equipent', 'necessary', 'for', 'the', 'biochemical', 'pro-', 'cedures', 'required,', 'with', 'the', 'exception', 'of', 'those', 'equipment', 'items', 'requested', 'in', 'the', 'budget.', 'Additional', 'studies', 'will', 'be', 'concerned', 'with', 'the', 'functional', 'role', 'of', 'differential', 'housing', 'as', 'a', 'model', 'for', 'the', 'induction', 'of', 'deficits', 'in', 'memory', 'consolidation', 'and', 'the', 'degree', 'to', 'which', 'both', 'acute', 'and', 'chronic', 'administration', 'of', 'nicotine', 'can', 'alter', 'these', 'deficits.', 'More', 'specific,', 'these', 'experiments', 'will', 'concern', 'a', 'comparison', 'of', 'memory', 'consolidation', 'upon', 'mice', 'housed', 'either', 'in', 'isolation', 'or', 'in', 'groups', 'following', 'weaning', ' ']