['CHARGE', 'NUMBER;', '2530', 'PROJECT', 'TITLE:', 'Smoke', 'Aerosol', 'Chemistry', 'PROJECT', 'LEADER:', 'Douglas', 'D.', 'McRae', 'PERIOD', 'COVERED:', 'May', '1,', '1983', '-', 'June', '30,', '1983', 'DATE', 'OF', 'REPORT:', 'July', '11,', '1983', '=', '.', 'Gas', 'Chromatography?', '_', 'ee', 'ee', 'oe', 'ane)', 'up', 'SR', '-', '=', '=', '=', 'Most', 'of', 'the', 'research', 'performed', 'in', 'this', 'period', 'has', 'been', 'done', 'in', 'cooperation', 'with', 'Projects', '2515', 'and', '2525.', 'The', 'First', 'is', 'a', 'continuation', 'of', 'the', '3-methylvaleric', 'acid', '(3-MVA)', 'release', 'study.', 'The', 'goal', 'was', 'to', 'find', 'a', 'good,', 'reliable', 'method', 'for', 'sweeping', '3-MVA', 'off', 'of', 'a', 'cigarette', 'and', 'into', 'a', 'capillary', 'GC', 'column', 'for', 'analysis.', 'Unfortunately,', 'it', 'appears', 'that', 'the', "compound's", 'solubility', 'in', 'water', 'causes', 'the', 'peak', 'to', 'spread', 'out', 'over', 'the', 'column', 'during', 'sweeping.', 'Therefore,', 'the', 'material', 'is', 'now', 'being', 'swept', 'onto', 'an', 'activated', 'charcoal', 'trap', 'off-line.', 'The', 'trapped', 'material', 'is', 'then', 'eluted', 'with', 'a', 'solvent', 'and', 'the', 'solution', 'is', 'chromatographed.', 'This', 'method', 'is', 'the', 'one', 'Frances', 'Hsu', 'uses', 'for', 'the', 'analysis', 'of', 'tobacco.', 'The', 'second', 'project', 'involved', 'helping', 'with', 'the', 'capillary', 'GC', 'quantitative', 'analysis', 'of', 'the', 'release', 'compounds', 'from', 'CR-1845', 'from', 'tobacco', 'filler', 'upon', 'smoking.', 'The', 'mainstream,', 'Sidestream,', 'and', 'butts', 'were', 'analyzed', 'for', 'the', 'presence', 'of', 'two', 'of', 'the', 'release', 'compounds', 'and', 'the', 'Starting', 'material.', 'A', 'method', 'was', 'developed', 'but', 'the', 'values', 'obtained', 'are', 'still', 'tentative', 'due', 'to', 'the', 'suspected', 'presence', 'of', 'a', 'compound', 'which', 'coelutes', 'with', 'the', 'internal', 'Standard,', 'The', 'third', 'project', 'was', 'the', 'analysis', 'of', 'aliphatic', 'amines', 'from', 'tobacco', 'smoke', '-in', 'cooperation', 'with', 'R.', 'Izac', 'and', 'J.', 'Naworal.', 'Various', 'columns', 'and', 'injection', 'techniques', 'are', 'being', 'tried,', '.', 'vd¢c?', '=', 'Two', 'smoking', 'runs', '(the', 'fourth', 'and', 'fifth)', 'for', 'the', 'vdc', 'project', 'were', 'performed', 'on', 'May', '9', 'and', '31,', '1983', 'i;', 'Cooperation', 'with', 'Project', '2525', '(3,4).', '4th', 'Run', '5th', 'Run', '(unfiltered)', '(filtered)', 'No.', 'Cigarettes', '-', '113', '120', 'Expected', 'activity', '.', '183.', '1uCi', '121.2uCi', 'Total', 'Mainstream', '28.0uCi', '18.7uCi', 'Total', 'Sidestream', '—', '60.9uCi', '56.8uCi', 'Butts', 'and', 'Ash', '~-', '65.1uCi', '29,8uCi', 'Hood', 'and', 'Smoking', 'Machina', '.', '4.3uCi', '3.1yCi', '%', 'Recovery', '86.5%', '89.4%', 'In', 'both', 'runs', 'a', 'high', 'volume', 'Floris?', 'trap', 'was', 'used', 'to', 'collect', 'the', 'activity', 'escaping', 'in', 'the', 'sidestream', 'gas', 'phase.', 'This', 'activity', 'was', '36.5uCi', 'and', '31.7uCi', 'for', 'the', '4th', 'and', '5th', 'runs,', 'respectively.', 'The', 'addition', 'of', 'this', 'new', 'trap', 'substantially', 'increased', 'the', 'total', 'recovery', 'of', 'the', 'initial', 'activity.', 'TLC', 'analysis', 'of', 'the', 'material', 'eluted', '",', 'bo', 'ie', ' ', 'Ms', 'Ge', 'ee:', 'ms', '62.D68S6TSOe¢']