['205', 'withheld', 'overnight', 'prior', 'to', 'necropsy.', 'No', 'chemical', 'analyses', 'of', 'feed', 'or', 'bedding', 'were', 'performed.', '8.2.6.', 'Experimental', 'Design', 'Three', 'groups', 'of', 'animals', 'were', 'exposed', 'to', 'smoke', 'from', 'the', 'NEW', 'CIGARETTE,', 'and', 'three', 'groups', 'to', 'smoke', 'from', 'the', 'reference', 'cigarette.', 'These', 'groups', 'represented', 'the', 'low-,', 'mid-', 'and', 'high-dose', 'exposure', 'groups.', 'The', 'actual', 'dilutions', 'of', 'the', 'mainstream', 'smoke', 'were', 'adjusted', 'to', 'provide', 'for', 'each', 'comparison', 'a', 'smoke', 'with', 'comparable', 'concentrations', 'of', 'either', 'TPM', 'or', 'nicotine', 'at', 'the', 'animal', 'ports.', 'In', 'addition', 'there', 'was', 'a', 'machine', 'control', 'group', 'placed', 'in', 'restraint', 'tubes,', 'but', 'exposed', 'only', 'to', 'dilution', 'air.', 'Another', 'group', 'of', 'animals', 'was', 'kept', 'as', 'room', 'controls.', '8.2.7.', 'In-Life', 'Data', 'Acquisition', 'Data', 'were', 'acquired', 'using', 'the', 'Xybion', 'Path/Tox', 'System', '(Xybion', 'Medical', 'Systems,', 'Cedar', 'Knolls,', 'NJ).', '8.2.7.1.', 'Gross', 'Observations', 'Animals', 'are', 'observed', 'on', 'each', 'exposure', 'day', 'as', 'they', 'were', 'being', 'transferred', 'from', 'their', 'cages', 'to', 'the', 'restrain', 'tubes,', 'and', 'when', 'they', 'were', 'transferred', 'back', 'to', 'their', 'cages.', 'Detailed', 'clinical', 'observations', 'were', 'made', 'on', 'each', 'animal', 'once', 'every', 'two', 'weeks,', 'before', 'each', 'exposure', 'and', '2', 'hours', 'after', 'the', 'end', 'of', 'the', 'exposure.', 'Data', 'were', 'key-entered', 'into', 'the', 'Xybion', 'System.', '8.2.7.2.', 'Early', 'Deaths', '/', 'Viability', 'Checks', 'Viability', 'checks', 'are', 'made', 'twice', 'daily', 'at', 'intervals', 'of', 'at', 'least', 'four', 'hours', '(five', 'days', 'per', 'week,', 'with', 'only', 'a', 'single', 'check', 'performed', 'on', 'weekends).', 'Complete', 'necropsies', 'with', 'tissue', 'collection', 'for', 'histopathology', 'are', 'performed', 'by', 'a', 'veterinary', 'pathologist', '(or', 'experienced', 'assistant)', 'on', 'all', 'animals', 'found', 'dead', 'or', 'in', 'a', 'moribund', 'condition.', 'If', 'necropsy', 'could', 'not', 'be', 'performed', 'immediately', 'after', 'the', 'animal', 'was', 'found,', 'the', 'carcass', 'was', 'stored', 'at', '+4', '°C', 'to', 'minimize', 'tissue', 'autolysis.', 'Animals', 'were', 'necropsied', 'no', 'later', 'than', '16', 'hours', 'after', 'being', 'found', 'dead.', 'Animals', 'that', 'died', 'during', 'the', 'first', 'week', 'of', 'exposure', 'were', 'not', 'necropsied.', 'They', 'were', 'replaced', 'by', 'comparable', 'animals', 'left', 'over', 'after', 'the', 'randomization', 'process', 'and', 're-identified', 'as', 'such.', 'No', 'other', 'early', 'deaths', 'were', 'replaced', 'by', 'reserve', 'animals.', '8.2.7.3.', 'Body', 'Weights;', 'Feed', 'Consumption', 'Individual', 'body', 'weights', 'were', 'determined', 'within', '48-hours', 'of', 'receipt,', 'at', 'randomization,', 'once', 'per', 'week', 'during', 'the', 'period', 'between', 'randomization', 'and', 'initiation', 'of', 'the', 'study,', 'and', 'once', 'eScl', 'Scsts']