[' ', '16.', 'and', 'its', 'metabolites,', 'as', 'these', 'are', 'related', 'to', 'memory', 'consolidation.', 'This', 'issue', 'is', 'given', 'preliminary', 'support', 'in', 'this', 'application', 'by', 'methodological', 'and', 'empirical', 'findings', 'from', 'our', 'laboratory,', 'which', 'suggest', 'the', 'feasibility', 'of', 'obtaining', 'reasona-', 'bly', 'purified', 'neuronal', 'and', 'glial', 'fractions', 'from', 'mouse', 'brain', 'and', 'demostrating', 'that', 'in', 'vivo', 'treatment,', 'resulting', 'in', 'retrograde', 'ammesia', 'in', 'mice,', 'exerts', 'a', 'differential', 'effect', 'at', 'the', 'cellular', 'level;', 'i.e.,', 'neurons', 'are', 'affected', 'over', 'a', 'differentiate', 'time', 'course', 'than', 'glia', 'following', 'ECS,', 'and', 'the', 'effect', 'in', 'glia', 'is', 'more', 'prolonged', 'than', 'that', 'in', 'neurons.', 'The', 'specific', 'effect', 'to', 'which', 'these', 'findings', 'are', 'in', 'alterations', 'in', '5-', 'hydroxyindoleacetic', 'acid', 'following', 'electroconvulsive', 'shock.', 'Through', 'the', 'utilization', 'of', 'the', 'fractionation', 'technique,', 'which', 'involves', 'a', 'discontinuous', 'sucrose-ficoll', 'den-', 'sity', 'gradient', 'centrifugation,', 'a', 'neuronal', 'and', 'glial', 'pool', 'can', 'be', 'separated', 'from', 'myelin', 'and', 'red', 'cells.', 'These', 'fractions', 'have', 'been', 'morphologically', 'verified', 'in', 'both', 'phase-', 'contrast', 'and', 'electron', 'microscopy', 'and', 'their', 'purity', 'has', 'been', 'verified', 'by', 'the', 'relative', 'rations', 'of', 'acetylcholinesterase', 'in', 'the', 'respective', 'fractions.', 'The', 'purpose', 'of', 'this', 'final', 'series', 'of', 'experiments', 'will', 'be', 'to', 'assess', 'the', 'effect', 'of', 'nicotine', 'and', 'its', 'meta-', 'bolites', 'on', 'cellular', 'levels', 'of', 'biogenic', 'amines,', 'and', 'study', 'the', 'interaction', 'of', 'drug', 'treatment', 'with', 'electroconvulsive', 'shock', 'upon', 'these', 'cellular', 'levels.', 'Specifically,', 'we', 'may', 'anticipate', 'that', 'cellular', 'changes,', 'which', 'parallel', 'retrograde', 'amnesia', 'pro-', 'duced', 'by', 'ECS,', 'will', 'be', 'blocked,', 'attenuated,', 'or', 'antagonized', 'by', 'nicotine', 'or', 'its', 'active', 'metabolites', 'under', 'these', 'conditions', 'where', 'these', 'behaviorally', 'modify', 'the', 'amensic', 'effect', 'of', 'elctroshock.', 'All', 'of', 'the', 'experimental', 'conditions', 'will', 'be', 'applied', 'in', 'vivo,', 'following', 'which', 'the', 'brains', 'will', 'be', 'removed,', 'prepared,', 'and', 'extracted,', 'as', 'previously', 'described,', 'with', 'the', 'addition', 'of', 'the', 'fractionation', 'procedure', 'for', 'isolation', 'of', 'neuro-', 'nal', 'and', 'glial', 'pools', 'in', 'several', 'of', 'the', 'areas', 'described.', 'These', 'studies', 'should', 'allow', 'for', 'a', 'considerable', 'degree', 'of', 'useful', 'data', 'to', 'emerge', 'regarding', 'differences', 'in', 'the', 'regional', 'cellular', 'response', 'in', 'the', 'central', 'nervous', 'system', 'to', 'nicotine', 'and', 'its', 'meta-', ' ']