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Viruses 2015, 7(1), 394-421; https://doi.org/10.3390/v7010394

The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus

1
Centro de investigación Tibaitatá, Corporación Colombiana de Investigación Agropecuaria CORPOICA, Km 14 Vía Mosquera 250047, Cundinamarca, Colombia
2
Laboratorio de Ingeniería Genética y Biología Celular y Molecular—Área Virosis de Insectos (LIGBCM—AVI), Dto. de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, Provincia de Buenos Aires, 1876, Argentina
3
Centro de Bioinformática, Instituto de Biotecnología, Universidad Nacional de Colombia. Avenida Carrera 30 # 45, Bogotá 11001000, Cundinamarca, Colombia
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: John Burand and Madoka Nakai
Received: 28 November 2014 / Accepted: 26 December 2014 / Published: 20 January 2015
(This article belongs to the Special Issue Insect Viruses and Their Use for Microbial Pest Control)
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Abstract

Spodoptera frugiperda (Lepidoptera: Noctuidae) is a major pest in maize crops in Colombia, and affects several regions in America. A granulovirus isolated from S. frugiperda (SfGV VG008) has potential as an enhancer of insecticidal activity of previously described nucleopolyhedrovirus from the same insect species (SfMNPV). The SfGV VG008 genome was sequenced and analyzed showing circular double stranded DNA of 140,913 bp encoding 146 putative ORFs that include 37 Baculoviridae core genes, 88 shared with betabaculoviruses, two shared only with betabaculoviruses from Noctuide insects, two shared with alphabaculoviruses, three copies of own genes (paralogs) and the other 14 corresponding to unique genes without representation in the other baculovirus species. Particularly, the genome encodes for important virulence factors such as 4 chitinases and 2 enhancins. The sequence analysis revealed the existence of eight homologous regions (hrs) and also suggests processes of gene acquisition by horizontal transfer including the SfGV VG008 ORFs 046/047 (paralogs), 059, 089 and 099. The bioinformatics evidence indicates that the genome donors of mentioned genes could be alpha- and/or betabaculovirus species. The previous reported ability of SfGV VG008 to naturally co-infect the same host with other virus show a possible mechanism to capture genes and thus improve its fitness. View Full-Text
Keywords: baculovirus; granulovirus; Spodoptera frugiperda; genome; recombination baculovirus; granulovirus; Spodoptera frugiperda; genome; recombination
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Cuartas, P.E.; Barrera, G.P.; Belaich, M.N.; Barreto, E.; Ghiringhelli, P.D.; Villamizar, L.F. The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus. Viruses 2015, 7, 394-421.

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{"cc_re__cc_re__cc_by_3_0": ["\">\n    <meta name=\"dc.creator\" content=\"Emiliano Barreto\">\n    <meta name=\"dc.creator\" content=\"Pablo Ghiringhelli\">\n    <meta name=\"dc.creator\" content=\"Laura Villamizar\">\n    \n    <meta name=\"dc.type\" content=\"Article\">\n    <meta name=\"dc.source\" content=\"Viruses 2015, Vol. 7, Pages 394-421\">\n    <meta name=\"dc.date\" content=\"2015-01-20\">\n    <meta name =\"dc.identifier\" content=\"10.3390/v7010394\">\n    \n    <meta name=\"dc.publisher\" content=\"Multidisciplinary Digital Publishing Institute\">\n    <meta name=\"dc.rights\" content=\"http://creativecommons.org/licenses/by/3.0/\">\n    <meta name=\"dc.format\" content=\"application/pdf\" >\n    <meta name=\"dc.language\" content=\"en\" >\n    <meta name=\"dc.description\" content=\"Spodoptera frugiperda (Lepidoptera: Noctuidae) is a major pest in maize crops in Colombia, and affects several regions in America. A granulovirus isolated from S. frugiperda (SfGV VG008) has potential as an enhancer of insecticidal activity of previously described nucleopolyhedrovirus from the same insect species (SfMNPV). The SfGV VG008 genome was sequence"], "cc_re__cc_re__cc_by_4_0": ["lues greater than 60% (A2; C2; D2). In all cases, the most related sequences are highlighted in bold letter. Moreover, 3D structures were predicted using LOMETS (A3; C3) or QUARQS (B3) or I-TASSER (C3) servers. SF2: SfMNPV 3AP2; SFG: SfGV VG 008; SLN: SpltNPV II.&lt;/p&gt;\n                                                            \"></a></div>\n\t\t\t\n\t\t\t<div class=\"licenseText in-tab\" style=\"padding: 1em 0;\">\n                                    This is an open access article distributed under the <a href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution License</a> which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).\n                \t\t\t</div>\n\n\t   </div>\n\n\t\t\t\t<div id=\"tabs-4\" class=\"ui-tabs-panel\">\n\t\t\t<a id=\"suppl_id\" name=\"supplementary\"></a>\n\t\t\t<h1>Supplementary material</h1>\n\t\t\t\t\t\t\n\t\t\t<div class=\"in-tab\">\n\t\t\t\t<ul style=\"margin:0; list-style: none;\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<li><a name=\"supplementary_file1\"></a><b>Supplemen"], "copyright__copyright__copyright": ["\nCuartas, P.E.; Barrera, G.P.; Belaich, M.N.; Barreto, E.; Ghiringhelli, P.D.; Villamizar, L.F. The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus. Viruses 2015, 7, 394-421.\n\nShow more citation formats Show less citations formats\n\nRelated Articles\n\nArticle Metrics\n\nArticle Access Statistics\n\n1\n\nComments\n\n[Return to top]\nViruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert\nBack to Top", "\nCuartas, P.E.; Barrera, G.P.; Belaich, M.N.; Barreto, E.; Ghiringhelli, P.D.; Villamizar, L.F. The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus. Viruses 2015, 7, 394-421.\n\nShow more citation formats Show less citations formats\n\nRelated Articles\n\nArticle Metrics\n\nArticle Access Statistics\n\n1\n\nComments\n\n[Return to top]\nViruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert\nBack to Top", "\nCuartas, P.E.; Barrera, G.P.; Belaich, M.N.; Barreto, E.; Ghiringhelli, P.D.; Villamizar, L.F. The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus. Viruses 2015, 7, 394-421.\n\nShow more citation formats Show less citations formats\n\nRelated Articles\n\nArticle Metrics\n\nArticle Access Statistics\n\n1\n\nComments\n\n[Return to top]\nViruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert\nBack to Top"]}