User:Stuart McKellar/Notebook/Bird Sex Testing/2012/10/19

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Electrophoresis Test

Objective: Try and visualise DNA on an agar gel. Method: Poured a 2% Gel pre-stained with GelRed 1:10,000. This was from a while ago so I may need to post stain it. Heated in microwave for a couple of minutes and then let cool. Poured gel, added 3 x 3.3ul of 300-10KB ladder. Added 1 x 5ul ladder in 4th lane. Ran gel at 60ma and 100V (volts as recommended).

Results: DNA had visibly moved in first few minutes. Gel box underneath was on but no visualisation yet. I may be using the wrong dye for this gel box but Gel Red should have an excitation peak at the wavelength. I am not sure what wavelength it emits at though.

No visible results. This could be for the following reasons:

  • The ladder is degraded
  • The GelRed is not emitting light at a visible frequency
  • There is not enough contrast
  • The buffer is at an incorrect pH and the DNA is not moving with the bromophenol blue

And that is all the reasons that I can think of.



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{"cc_re__cc_re__cc_by_sa_2_5": ["a href=\"/wiki/OpenWetWare:How_to_join\">\n\t\t\t<img style=\"margin:2px;\" \n\t\t\t\tsrc=\"http://openwetware.org/images/0/03/88x31_JoinOWW.png\" border=0 /></a>\n                </div>\n        </div>\n\t\t\t\t\t\t\t</div>\n\n\t\t\t</div><!-- end of the left column -->\n\t\t\n\t\t\n\t\t\t<div class=\"visualClear\"></div>\n\t\t\t<div id=\"footer\">\n\t\t\t\t<div id=\"f-poweredbyico\"><a href=\"http://www.mediawiki.org/\"><img src=\"/skins/common/images/poweredby_mediawiki_88x31.png\" alt=\"Powered by MediaWiki\" /></a></div>\n\t\t\t\t<div id=\"f-copyrightico\"><a href=\"http://creativecommons.org/licenses/by-sa/2.5/\"><img src=\"/somerights20.png\" alt='GNU FDL or Creative Commons BY-SA' /></a></div>\n\t\t\t<ul id=\"f-list\">\n\t\t\t\t<li id=\"lastmod\"> This page was last modified on 19 October 2012, at 08:10.</li>\n\t\t\t\t<li id=\"viewcount\">This page has been accessed 281 times.</li>\n\t\t\t\t<li id=\"copyright\">Content is available under <a href=\"/wiki/Copyright\" title=\"Copyright\">GNU FDL or Creative Commons BY-SA</a>.</li>\n\t\t\t\t<li id=\"privacy\"><a href=\"/wiki/OpenWetWare:Privacy_policy\" title=\"OpenWetWare:Privacy policy\">Privacy p"], "copyright__copyright__copyright": ["t. I may be using the wrong dye for this gel box but Gel Red should have an excitation peak at the wavelength. I am not sure what wavelength it emits at though.\n\nNo visible results. This could be for the following reasons:\n\n  \u2022 The ladder is degraded\n  \u2022 The GelRed is not emitting light at a visible frequency\n  \u2022 There is not enough contrast\n  \u2022 The buffer is at an incorrect pH and the DNA is not moving with the bromophenol blue\n\nAnd that is all the reasons that I can think of.\n\n\n\nPersonal tools", "t. I may be using the wrong dye for this gel box but Gel Red should have an excitation peak at the wavelength. I am not sure what wavelength it emits at though.\n\nNo visible results. This could be for the following reasons:\n\n  \u2022 The ladder is degraded\n  \u2022 The GelRed is not emitting light at a visible frequency\n  \u2022 There is not enough contrast\n  \u2022 The buffer is at an incorrect pH and the DNA is not moving with the bromophenol blue\n\nAnd that is all the reasons that I can think of.\n\n\n\nPersonal tools", "t. I may be using the wrong dye for this gel box but Gel Red should have an excitation peak at the wavelength. I am not sure what wavelength it emits at though.\n\nNo visible results. This could be for the following reasons:\n\n  \u2022 The ladder is degraded\n  \u2022 The GelRed is not emitting light at a visible frequency\n  \u2022 There is not enough contrast\n  \u2022 The buffer is at an incorrect pH and the DNA is not moving with the bromophenol blue\n\nAnd that is all the reasons that I can think of.\n\n\n\nPersonal tools", "t. I may be using the wrong dye for this gel box but Gel Red should have an excitation peak at the wavelength. I am not sure what wavelength it emits at though.\n\nNo visible results. This could be for the following reasons:\n\n  \u2022 The ladder is degraded\n  \u2022 The GelRed is not emitting light at a visible frequency\n  \u2022 There is not enough contrast\n  \u2022 The buffer is at an incorrect pH and the DNA is not moving with the bromophenol blue\n\nAnd that is all the reasons that I can think of.\n\n\n\nPersonal tools", "t. I may be using the wrong dye for this gel box but Gel Red should have an excitation peak at the wavelength. I am not sure what wavelength it emits at though.\n\nNo visible results. This could be for the following reasons:\n\n  \u2022 The ladder is degraded\n  \u2022 The GelRed is not emitting light at a visible frequency\n  \u2022 There is not enough contrast\n  \u2022 The buffer is at an incorrect pH and the DNA is not moving with the bromophenol blue\n\nAnd that is all the reasons that I can think of.\n\n\n\nPersonal tools", "t. I may be using the wrong dye for this gel box but Gel Red should have an excitation peak at the wavelength. I am not sure what wavelength it emits at though.\n\nNo visible results. This could be for the following reasons:\n\n  \u2022 The ladder is degraded\n  \u2022 The GelRed is not emitting light at a visible frequency\n  \u2022 There is not enough contrast\n  \u2022 The buffer is at an incorrect pH and the DNA is not moving with the bromophenol blue\n\nAnd that is all the reasons that I can think of.\n\n\n\nPersonal tools"]}