ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
K Bramareswara Rao1, SR Krishna Motukuri1 , K Arun Kumar2, CHVN Praveen Babu2 and Vaibhav Pathak2
1Department of Biotechnology, Koneru Lakshmaiah Education Foundtion, Guntur,
Andhra Pradesh-522502, India.
2Department of Crop Improvement, Rasi Seeds Pvt Ltd, Toopran, Hyderabad,
Telangana-502334, India.
J Pure Appl Microbiol. 2021;15(2):752-758 | Article Number: 6628
https://doi.org/10.22207/JPAM.15.2.27 | © The Author(s). 2021
Received: 09/09/2020 | Accepted: 03/04/2021 | Published: 26/05/2021
Abstract

Pearl Millet leaf Blast caused by Pyricularia grisea [teleomorph: Magnaporthe grisea], is spreading at an alarming rate in the major Pearl millet growing geographies of India effecting grain yield and green fodder yield. Blast isolates collected from Jaipur, Alwar and Toopran regions of India and virulence study conducted to identify the most virulent isolate among the three isolates. Artificial screening for Blast conducted on a raised bed method utilising uniform Blast Nursery (UBN) method.  Eleven Pearl Millet genotypes (ICMB01333, ICMB03444, ICMB03555, ICMB06111, ICMB95444, ICMB11666, ICMB14333, ICMB14666, ICMB97111, ICMR12888 and ICMR06444) were screened with three blast isolates utilising artificial screening method. Among the eleven genotypes, ten genotypes were showing susceptible to Jaipur isolate indicating that the Jaipur isolate having highest virulence among the three isolates. To identify Blast resistant donors for Jaipur isolate, a set of 93 genotypes containing of 45 maintainer lines and 48 restorer lines were screened under both UBN and field conditions (Jaipur, Rajasthan). Among all the lines evaluated, five lines are showing resistant reaction for Jaipur isolate with disease score less than 1.9. ICMR06444 from restorer background and IC414K14B5, IC594K16B5, RBB037 and IC6912K18B from maintainer background are identified as resistant lines. Identified lines can be utilised in pearl millet hybrid breeding programme.

Keywords

Pearl Millet, Blast, Screening, Restorer, Maintainer, Resistance

Article Metrics

Article View: 560

Share This Article

© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
{"cc_re__cc_re__cc_by_4_0": ["rtical-align: baseline;\r\n                }\n</style><div class=\"wpb_wrapper\"><div class=\"wpb_wrapper wpb_text_column td_block_wrap td_block_wrap vc_column_text vc_custom_1611211515598 td_uid_49_6575a3871f574_rand  td-pb-border-top td_block_template_11\" data-td-block-uid=\"td_uid_49_6575a3871f574\"><div class=\"td-block-title-wrap\"></div><div class=\"td-fix-index\"><p><span style=\"font-size: 10pt;\">\u00a9 The Author(s) 2021. <strong>Open Access</strong>. This article is distributed under the terms of the\u00a0<a href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\" rel=\"noopener noreferrer\">Creative Commons Attribution 4.0 International License</a>\u00a0which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.</span></p>\n</div></div></div></div></div></div>\n</div></div></div></div></div></div></div></div><div class=\"addtoany_share_save_container addtoany_content addtoany_content_bottom\"><div class=", "apper td_block_wrap vc_raw_html td_uid_53_6575a38721534_rand \"><div class=\"td-fix-index\"><a href=\"https://microbiologyjournal.org/\"><img class=\"img img-responsive\" src=\"https://microbiologyjournal.org/wp-content/uploads/2020/02/bottom-Logo-2.png\" /> </a>\nPrint ISSN: <a href=\"https://portal.issn.org/resource/ISSN/0973-7510\" target=\"_blank\" rel=\"noopener\">0973-7510</a> <br>\nOnline ISSN: <a href=\"https://portal.issn.org/resource/ISSN/2581-690X\" target=\"_blank\" rel=\"noopener\">2581-690X</a> <br><br>\n<a href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\" title=\"Creative Commons Attribution 4.0 International (CC BY 4.0) License.\" rel=\"noopener\"><img class=\"img img-responsive\" src=\"https://microbiologyjournal.org/wp-content/uploads/2021/01/creativecommons.png\" /></a><br>\n<p style=\"font-size: 11px !important;margin-bottom: 5px;\n\">All Journal of Pure and Applied Microbiology articles are published under the terms of the</p>\n<a target=\"_blank\" href=\"https://creativecommons.org/licenses/by/4.0/\" style=\"font-size: 11px;color: darkblue;font-weight: bold;\" rel=\"noopener\">Creative Commons Attribution 4.0 International (CC BY 4.0) License.</a></", "\" target=\"_blank\" rel=\"noopener\">2581-690X</a> <br><br>\n<a href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\" title=\"Creative Commons Attribution 4.0 International (CC BY 4.0) License.\" rel=\"noopener\"><img class=\"img img-responsive\" src=\"https://microbiologyjournal.org/wp-content/uploads/2021/01/creativecommons.png\" /></a><br>\n<p style=\"font-size: 11px !important;margin-bottom: 5px;\n\">All Journal of Pure and Applied Microbiology articles are published under the terms of the</p>\n<a target=\"_blank\" href=\"https://creativecommons.org/licenses/by/4.0/\" style=\"font-size: 11px;color: darkblue;font-weight: bold;\" rel=\"noopener\">Creative Commons Attribution 4.0 International (CC BY 4.0) License.</a></div></div></div></div><div class=\"vc_column td_uid_54_6575a38721561_rand  wpb_column vc_column_container tdc-column td-pb-span9\">\n<style scoped>\n\n/* custom css */\n.td_uid_54_6575a38721561_rand {\r\n                    vertical-align: baseline;\r\n                }\n</style><div class=\"wpb_wrapper\"><div class=\"vc_row_inner td_uid_55_6575a38721620_rand foot2 vc_row vc_inner wpb_row td-pb-row\">\n<style scoped>\n\n/* custom css */\n.td_"], "copyright__copyright__copyright": ["t, Blast, Screening, Restorer, Maintainer, Resistance\n\nArticle Metrics\n\nArticle View: 560\n\nShare This Article\n\n\u00a9 The Author(s) 2021. Open Access. This article is distributed under the terms of the\u00a0Creative Commons Attribution 4.0 International License\u00a0which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.", "t, Blast, Screening, Restorer, Maintainer, Resistance\n\nArticle Metrics\n\nArticle View: 560\n\nShare This Article\n\n\u00a9 The Author(s) 2021. Open Access. This article is distributed under the terms of the\u00a0Creative Commons Attribution 4.0 International License\u00a0which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.", "t, Blast, Screening, Restorer, Maintainer, Resistance\n\nArticle Metrics\n\nArticle View: 560\n\nShare This Article\n\n\u00a9 The Author(s) 2021. Open Access. This article is distributed under the terms of the\u00a0Creative Commons Attribution 4.0 International License\u00a0which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.", "t, Blast, Screening, Restorer, Maintainer, Resistance\n\nArticle Metrics\n\nArticle View: 560\n\nShare This Article\n\n\u00a9 The Author(s) 2021. Open Access. This article is distributed under the terms of the\u00a0Creative Commons Attribution 4.0 International License\u00a0which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.", "t, Blast, Screening, Restorer, Maintainer, Resistance\n\nArticle Metrics\n\nArticle View: 560\n\nShare This Article\n\n\u00a9 The Author(s) 2021. Open Access. This article is distributed under the terms of the\u00a0Creative Commons Attribution 4.0 International License\u00a0which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.", "t, Blast, Screening, Restorer, Maintainer, Resistance\n\nArticle Metrics\n\nArticle View: 560\n\nShare This Article\n\n\u00a9 The Author(s) 2021. Open Access. This article is distributed under the terms of the\u00a0Creative Commons Attribution 4.0 International License\u00a0which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.", "t, Blast, Screening, Restorer, Maintainer, Resistance\n\nArticle Metrics\n\nArticle View: 560\n\nShare This Article\n\n\u00a9 The Author(s) 2021. Open Access. This article is distributed under the terms of the\u00a0Creative Commons Attribution 4.0 International License\u00a0which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.", "t, Blast, Screening, Restorer, Maintainer, Resistance\n\nArticle Metrics\n\nArticle View: 560\n\nShare This Article\n\n\u00a9 The Author(s) 2021. Open Access. This article is distributed under the terms of the\u00a0Creative Commons Attribution 4.0 International License\u00a0which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.", "t, Blast, Screening, Restorer, Maintainer, Resistance\n\nArticle Metrics\n\nArticle View: 560\n\nShare This Article\n\n\u00a9 The Author(s) 2021. Open Access. This article is distributed under the terms of the\u00a0Creative Commons Attribution 4.0 International License\u00a0which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made."]}