IGEM:UC Berkeley/2006/DNAGelPrep

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Pouring Gels

  1. Fill 500ml beaker to ~350ml with cold water
  2. Mix in 6g agarose
  3. Give to chris to microwave, or if feeling brave, 30second intervals lightly swirling in between until liquid is clear
  4. Add 10ml 50x TAE Buffer
  5. Fill 500ml beaker to 600ml with cold h20, so a bit above the 500 mark but not so over flowing
  6. Add 50ul 10,000x SYBR Green (Bottle says ‘SYBR Safe DNA gel stain’, and liquid is very red)
  7. Pour into gel mold

-DLK_070306

Personal tools
{"cc_re__cc_re__cc_by_sa_2_5": ["a href=\"/wiki/OpenWetWare:How_to_join\">\n\t\t\t<img style=\"margin:2px;\" \n\t\t\t\tsrc=\"http://openwetware.org/images/0/03/88x31_JoinOWW.png\" border=0 /></a>\n                </div>\n        </div>\n\t\t\t\t\t\t\t</div>\n\n\t\t\t</div><!-- end of the left column -->\n\t\t\n\t\t\n\t\t\t<div class=\"visualClear\"></div>\n\t\t\t<div id=\"footer\">\n\t\t\t\t<div id=\"f-poweredbyico\"><a href=\"http://www.mediawiki.org/\"><img src=\"/skins/common/images/poweredby_mediawiki_88x31.png\" alt=\"Powered by MediaWiki\" /></a></div>\n\t\t\t\t<div id=\"f-copyrightico\"><a href=\"http://creativecommons.org/licenses/by-sa/2.5/\"><img src=\"/somerights20.png\" alt='GNU FDL or Creative Commons BY-SA' /></a></div>\n\t\t\t<ul id=\"f-list\">\n\t\t\t\t<li id=\"lastmod\"> This page was last modified on 3 July 2006, at 11:21.</li>\n\t\t\t\t<li id=\"viewcount\">This page has been accessed 1,499 times.</li>\n\t\t\t\t<li id=\"copyright\">Content is available under <a href=\"/wiki/Copyright\" title=\"Copyright\">GNU FDL or Creative Commons BY-SA</a>.</li>\n\t\t\t\t<li id=\"privacy\"><a href=\"/wiki/OpenWetWare:Privacy_policy\" title=\"OpenWetWare:Privacy policy\">Privacy pol"], "copyright__copyright__copyright": ["tion, search\n\nPouring Gels\n\n  1. Fill 500ml beaker to ~350ml with cold water\n  2. Mix in 6g agarose\n  3. Give to chris to microwave, or if feeling brave, 30second intervals lightly swirling in between until liquid is clear\n  4. Add 10ml 50x TAE Buffer\n  5. Fill 500ml beaker to 600ml with cold h20, so a bit above the 500 mark but not so over flowing\n  6. Add 50ul 10,000x SYBR Green (Bottle says \u2018SYBR Safe DNA gel stain\u2019, and liquid is very red)\n  7. Pour into gel mold\n\n-DLK_070306\n\nPersonal tools", "tion, search\n\nPouring Gels\n\n  1. Fill 500ml beaker to ~350ml with cold water\n  2. Mix in 6g agarose\n  3. Give to chris to microwave, or if feeling brave, 30second intervals lightly swirling in between until liquid is clear\n  4. Add 10ml 50x TAE Buffer\n  5. Fill 500ml beaker to 600ml with cold h20, so a bit above the 500 mark but not so over flowing\n  6. Add 50ul 10,000x SYBR Green (Bottle says \u2018SYBR Safe DNA gel stain\u2019, and liquid is very red)\n  7. Pour into gel mold\n\n-DLK_070306\n\nPersonal tools", "tion, search\n\nPouring Gels\n\n  1. Fill 500ml beaker to ~350ml with cold water\n  2. Mix in 6g agarose\n  3. Give to chris to microwave, or if feeling brave, 30second intervals lightly swirling in between until liquid is clear\n  4. Add 10ml 50x TAE Buffer\n  5. Fill 500ml beaker to 600ml with cold h20, so a bit above the 500 mark but not so over flowing\n  6. Add 50ul 10,000x SYBR Green (Bottle says \u2018SYBR Safe DNA gel stain\u2019, and liquid is very red)\n  7. Pour into gel mold\n\n-DLK_070306\n\nPersonal tools", "tion, search\n\nPouring Gels\n\n  1. Fill 500ml beaker to ~350ml with cold water\n  2. Mix in 6g agarose\n  3. Give to chris to microwave, or if feeling brave, 30second intervals lightly swirling in between until liquid is clear\n  4. Add 10ml 50x TAE Buffer\n  5. Fill 500ml beaker to 600ml with cold h20, so a bit above the 500 mark but not so over flowing\n  6. Add 50ul 10,000x SYBR Green (Bottle says \u2018SYBR Safe DNA gel stain\u2019, and liquid is very red)\n  7. Pour into gel mold\n\n-DLK_070306\n\nPersonal tools", "tion, search\n\nPouring Gels\n\n  1. Fill 500ml beaker to ~350ml with cold water\n  2. Mix in 6g agarose\n  3. Give to chris to microwave, or if feeling brave, 30second intervals lightly swirling in between until liquid is clear\n  4. Add 10ml 50x TAE Buffer\n  5. Fill 500ml beaker to 600ml with cold h20, so a bit above the 500 mark but not so over flowing\n  6. Add 50ul 10,000x SYBR Green (Bottle says \u2018SYBR Safe DNA gel stain\u2019, and liquid is very red)\n  7. Pour into gel mold\n\n-DLK_070306\n\nPersonal tools", "tion, search\n\nPouring Gels\n\n  1. Fill 500ml beaker to ~350ml with cold water\n  2. Mix in 6g agarose\n  3. Give to chris to microwave, or if feeling brave, 30second intervals lightly swirling in between until liquid is clear\n  4. Add 10ml 50x TAE Buffer\n  5. Fill 500ml beaker to 600ml with cold h20, so a bit above the 500 mark but not so over flowing\n  6. Add 50ul 10,000x SYBR Green (Bottle says \u2018SYBR Safe DNA gel stain\u2019, and liquid is very red)\n  7. Pour into gel mold\n\n-DLK_070306\n\nPersonal tools"]}