PCR-ISH

In an effort to run in situ hybridizations (ISH) on both coral and sea star samples, we tested 9 sets of primers today and multiple temperatures.

I ran Eubacterial primers, 27F & 1522R, and Ehrlichiae primers on coral samples. The coral DNA is from Sarah’s A. cervicornis white band samples. Below are the master mixes and annealing temps that were tested for the Ehrlichiae outside of the 55C temperature that we ran the Eubacteria primers on.
{"cc_re__cc_re__cc_by_3_0": ["t 3 years ago</a></span>  </li>\n<li class=\"lang-en\">RT @birdsfmthetrain: @EIMD_fhl @CClosek @dirtygeew @sr320 @lisa_418 This video still cracks me up every time I see it #teamzoozoo<br /><span class='wpt-tweet-time'><a href=\"http://twitter.com/EIMD_fhl/status/652086290247221248\">about 3 years ago</a></span>  </li>\n</ul>\n</aside>\t\t</div><!-- #secondary -->\n\t\t</div><!-- #main .wrapper -->\n\t<footer id=\"colophon\" role=\"contentinfo\">\n\t\t<div class=\"site-info\">\n\t\t\t\t\t\tDistributed under the terms of the <a href=\"http://creativecommons.org/licenses/by/3.0/\">Creative Commons Attribution License</a>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Proudly powered by <a href=\"http://onsnetwork.org\">ONSnetwork.org</a>, <a href=\"http://scifundchallenge.org\">The #SciFund Challenge</a>, the <a href=\"http://opensciencefederation.com\">Open Science Federation</a>, and <a href=\"http://wordpress.org/\" title=\"Semantic Personal Publishing Platform\">WordPress</a>.\n\t\t</div><!-- .si"], "copyright__copyright__copyright": ["PCR-ISH\n\nIn an effort to run in situ hybridizations (ISH) on both coral and sea star samples, we tested 9 sets of primers today and multiple temperatures.\n\nI ran Eubacterial primers, 27F & 1522R, and Ehrlichiae primers on coral samples. The coral DNA is from Sarah\u2019s\u00a0A. cervicornis white band samples. Below are the master mixes and annealing temps that were tested for the Ehrlichiae outside of the 55C temperature that we ran the Eubacteria primers on."]}