RESEARCH ARTICLE


Antimicrobial Activity of Cultivable Endophytic Fungi Associated with Hancornia Speciosa Gomes Bark



Mardonny Bruno de Oliveira Chagas1, Irailton Prazeres dos Santos1, Luis Claudio Nascimento da Silva2, Maria Tereza dos Santos Correia1, Janete Magali de Araújo3, Marilene da Silva Cavalcanti4, Vera Lucia de Menezes Lima1, *
1 Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, S/Nº, Cidade Universitária, Recife, PE, 50670-420, Brazil
2 Programa de Pós-graduação em Biologia Parasitária, Universidade Ceuma, Rua Josué Montello, São Luís, MA, 65075-120, Brazil
3 Departamento de Antibióticos, Universidade Federal de Pernambuco, Av. Nelson Chaves, S/N, Cidade Universitária, Recife, PE, 50670-420, Brazil
4 Departamento de Micologia, Universidade Federal de Pernambuco, Av. Nelson Chaves, Cidade Universitária, S/N, Recife, PE, 50670-420, Brazil


Article Metrics

CrossRef Citations:
24
Total Statistics:

Full-Text HTML Views: 5333
Abstract HTML Views: 2497
PDF Downloads: 1353
ePub Downloads: 1029
Total Views/Downloads: 10212
Unique Statistics:

Full-Text HTML Views: 2733
Abstract HTML Views: 1396
PDF Downloads: 788
ePub Downloads: 565
Total Views/Downloads: 5482



© 2017 Chagas et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, S/Nº, Cidade Universitária, Recife, PE, 50670-420, Brazil; Tel: +5581-32718540; Fax: +5581-32718541; E-mail: lima.vera.ufpe@gmail.com


Abstract

Introduction:

In this study, we evaluated the antimicrobial potential of cultivable endophytic fungi associated with Hancornia speciosa Gomes stem bark.

Methods and Materials:

Plant samples were collected in rainy (July 2010) and dry (January 2011) seasons. In total, 116 endophytic fungi strains were isolated from 90 fragments (64.4% frequency of colonization). Higher fungi frequency was observed in the rainy season (84.4%). The strains were grouped into 14 species; the most frequent were Phoma cava (13.8%), Colletotrichum gloeosporioides (12.1%), and Lasiodiplodia theobromae (11.2%). Fungal diversity was similar in both the seasons. Among the 116 strains, 39 (33.6%) showed antimicrobial activity in preliminary screening. The ten most active isolates were subjected to semi-solid fermentation using rice or corn as substrates. Methanolic extracts were obtained from each fermentation medium and the minimum inhibitory (MIC) and minimum microbicide concentrations (MMC) were determined.

Results:

The best antimicrobial results (MIC < 100 µg/mL) were observed for fungi strains grown in rice medium: Aspergillus niger FHS061 against Proteus mirabilis (MIC = 19 µg/mL) and Staphylococcus aureus (MIC = 39 µg/mL). These strains also showed good results when cultivated in corn medium against P. mirabilis (MIC = 78 µg/mL).

Conclusion:

Thus, the stem bark of H. speciosa harbors diverse endophytic fungi with antimicrobial potential.

Keywords: Fungi diversity, Natural compounds, Semi-solid fermentation, Aspergillus niger, Fusarium solani, Proteus mirabilis.
{"cc_re__cc_re__cc_by_4_0_le": ["commons.org/licenses/by/4.0/','mywindow');\" style=\"cursor: pointer;\"><img alt=\"Creative Commons License\" style=\"border-width:0\" src=\"https://i.creativecommons.org/l/by/4.0/80x15.png\"></div>\n<strong>\u00a9 2017 Chagas <em>et al</em>.\n</strong><br><br><span class=\"gen\" style=\"text-transform:capitalize\">open-access license: </span>This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: <a target=\"xrefwindow\" href=\"https://creativecommons.org/licenses/by/4.0/legalcode\">https://creativecommons.org/licenses/by/4.0/legalcode</a>. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</div>\n<br><div class=\"author_notes\"><span class=\"capture-id\"><a name=\"cor1\"></a><sup>*</sup> Address correspondence to this author at the Departamento de Bioqu\u00edmica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, S/N\u00ba, Cidade Universit\u00e1ria, Recife, PE, 50670-420, Brazil; Tel: +5581-3271"], "copyright__copyright__copyright": [" for fungi strains grown in rice medium: Aspergillus niger FHS061 against Proteus mirabilis (MIC = 19 \u00b5g/mL) and Staphylococcus aureus (MIC = 39 \u00b5g/mL). These strains also showed good results when cultivated in corn medium against P. mirabilis (MIC = 78 \u00b5g/mL).\n\nConclusion:\n\nThus, the stem bark of H. speciosa harbors diverse endophytic fungi with antimicrobial potential.\n\nKeywords: Fungi diversity, Natural compounds, Semi-solid fermentation, Aspergillus niger, Fusarium solani, Proteus mirabilis.", " for fungi strains grown in rice medium: Aspergillus niger FHS061 against Proteus mirabilis (MIC = 19 \u00b5g/mL) and Staphylococcus aureus (MIC = 39 \u00b5g/mL). These strains also showed good results when cultivated in corn medium against P. mirabilis (MIC = 78 \u00b5g/mL).\n\nConclusion:\n\nThus, the stem bark of H. speciosa harbors diverse endophytic fungi with antimicrobial potential.\n\nKeywords: Fungi diversity, Natural compounds, Semi-solid fermentation, Aspergillus niger, Fusarium solani, Proteus mirabilis.", " for fungi strains grown in rice medium: Aspergillus niger FHS061 against Proteus mirabilis (MIC = 19 \u00b5g/mL) and Staphylococcus aureus (MIC = 39 \u00b5g/mL). These strains also showed good results when cultivated in corn medium against P. mirabilis (MIC = 78 \u00b5g/mL).\n\nConclusion:\n\nThus, the stem bark of H. speciosa harbors diverse endophytic fungi with antimicrobial potential.\n\nKeywords: Fungi diversity, Natural compounds, Semi-solid fermentation, Aspergillus niger, Fusarium solani, Proteus mirabilis.", " for fungi strains grown in rice medium: Aspergillus niger FHS061 against Proteus mirabilis (MIC = 19 \u00b5g/mL) and Staphylococcus aureus (MIC = 39 \u00b5g/mL). These strains also showed good results when cultivated in corn medium against P. mirabilis (MIC = 78 \u00b5g/mL).\n\nConclusion:\n\nThus, the stem bark of H. speciosa harbors diverse endophytic fungi with antimicrobial potential.\n\nKeywords: Fungi diversity, Natural compounds, Semi-solid fermentation, Aspergillus niger, Fusarium solani, Proteus mirabilis.", " for fungi strains grown in rice medium: Aspergillus niger FHS061 against Proteus mirabilis (MIC = 19 \u00b5g/mL) and Staphylococcus aureus (MIC = 39 \u00b5g/mL). These strains also showed good results when cultivated in corn medium against P. mirabilis (MIC = 78 \u00b5g/mL).\n\nConclusion:\n\nThus, the stem bark of H. speciosa harbors diverse endophytic fungi with antimicrobial potential.\n\nKeywords: Fungi diversity, Natural compounds, Semi-solid fermentation, Aspergillus niger, Fusarium solani, Proteus mirabilis.", " for fungi strains grown in rice medium: Aspergillus niger FHS061 against Proteus mirabilis (MIC = 19 \u00b5g/mL) and Staphylococcus aureus (MIC = 39 \u00b5g/mL). These strains also showed good results when cultivated in corn medium against P. mirabilis (MIC = 78 \u00b5g/mL).\n\nConclusion:\n\nThus, the stem bark of H. speciosa harbors diverse endophytic fungi with antimicrobial potential.\n\nKeywords: Fungi diversity, Natural compounds, Semi-solid fermentation, Aspergillus niger, Fusarium solani, Proteus mirabilis.", " for fungi strains grown in rice medium: Aspergillus niger FHS061 against Proteus mirabilis (MIC = 19 \u00b5g/mL) and Staphylococcus aureus (MIC = 39 \u00b5g/mL). These strains also showed good results when cultivated in corn medium against P. mirabilis (MIC = 78 \u00b5g/mL).\n\nConclusion:\n\nThus, the stem bark of H. speciosa harbors diverse endophytic fungi with antimicrobial potential.\n\nKeywords: Fungi diversity, Natural compounds, Semi-solid fermentation, Aspergillus niger, Fusarium solani, Proteus mirabilis.", " for fungi strains grown in rice medium: Aspergillus niger FHS061 against Proteus mirabilis (MIC = 19 \u00b5g/mL) and Staphylococcus aureus (MIC = 39 \u00b5g/mL). These strains also showed good results when cultivated in corn medium against P. mirabilis (MIC = 78 \u00b5g/mL).\n\nConclusion:\n\nThus, the stem bark of H. speciosa harbors diverse endophytic fungi with antimicrobial potential.\n\nKeywords: Fungi diversity, Natural compounds, Semi-solid fermentation, Aspergillus niger, Fusarium solani, Proteus mirabilis.", " for fungi strains grown in rice medium: Aspergillus niger FHS061 against Proteus mirabilis (MIC = 19 \u00b5g/mL) and Staphylococcus aureus (MIC = 39 \u00b5g/mL). These strains also showed good results when cultivated in corn medium against P. mirabilis (MIC = 78 \u00b5g/mL).\n\nConclusion:\n\nThus, the stem bark of H. speciosa harbors diverse endophytic fungi with antimicrobial potential.\n\nKeywords: Fungi diversity, Natural compounds, Semi-solid fermentation, Aspergillus niger, Fusarium solani, Proteus mirabilis."]}