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Mar. Drugs 2017, 15(6), 156; https://doi.org/10.3390/md15060156

Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine

1
College of Environment, Zhejiang University of Technology, Hangzhou 310014, China
2
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
3
Nanjing Yuanheng Institute for Environmental Studies Co., Ltd., Nanjing 210049, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editors: Vassilios Roussis, Efstathia Ioannou and Peer B. Jacobson
Received: 14 January 2017 / Revised: 10 April 2017 / Accepted: 25 May 2017 / Published: 31 May 2017
(This article belongs to the Special Issue Biotransformations Utilizing Marine/Marine-Derived Bacteria and Fungi)
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Abstract

The presence of nicotine and nicotinic acid (NA) in the marine environment has caused great harm to human health and the natural environment. Therefore, there is an urgent need to use efficient and economical methods to remove such pollutants from the environment. In this study, a nicotine and NA-degrading bacterium—strain JQ581—was isolated from sediment from the East China Sea and identified as a member of Pseudomonas putida based on morphology, physio-biochemical characteristics, and 16S rDNA gene analysis. The relationship between growth and nicotine/NA degradation suggested that strain JQ581 was a good candidate for applications in the bioaugmentation treatment of nicotine/NA contamination. The degradation intermediates of nicotine are pseudooxynicotine (PN) and 3-succinoyl-pyridine (SP) based on UV, high performance liquid chromatography, and liquid chromatography-mass spectrometry analyses. However, 6-hydroxy-3-succinoyl-pyridine (HSP) was not detected. NA degradation intermediates were identified as 6-hydroxynicotinic acid (6HNA). The whole genome of strain JQ581 was sequenced and analyzed. Genome sequence analysis revealed that strain JQ581 contained the gene clusters for nicotine and NA degradation. This is the first report where a marine-derived Pseudomonas strain had the ability to degrade nicotine and NA simultaneously. View Full-Text
Keywords: nicotine; nicotinic acid; biodegradation; genome; Pseudomonas putida; marine-derived bacterium nicotine; nicotinic acid; biodegradation; genome; Pseudomonas putida; marine-derived bacterium
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Li, A.; Qiu, J.; Chen, D.; Ye, J.; Wang, Y.; Tong, L.; Jiang, J.; Chen, J. Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine. Mar. Drugs 2017, 15, 156.

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{"cc_re__cc_re__cc_by_3_0": ["ent=\"Jiexu Ye\">\n<meta name=\"dc.creator\" content=\"Yuhong Wang\">\n<meta name=\"dc.creator\" content=\"Lu Tong\">\n<meta name=\"dc.creator\" content=\"Jiandong Jiang\">\n<meta name=\"dc.creator\" content=\"Jianmeng Chen\">\n<meta name=\"dc.type\" content=\"Article\">\n<meta name=\"dc.source\" content=\"Marine Drugs 2017, Vol. 15, Page 156\">\n<meta name=\"dc.date\" content=\"2017-05-31\">\n<meta name=\"dc.identifier\" content=\"10.3390/md15060156\">\n<meta name=\"dc.publisher\" content=\"Multidisciplinary Digital Publishing Institute\">\n<meta name=\"dc.rights\" content=\"http://creativecommons.org/licenses/by/3.0/\">\n<meta name=\"dc.format\" content=\"application/pdf\">\n<meta name=\"dc.language\" content=\"en\">\n<meta name=\"dc.description\" content=\"The presence of nicotine and nicotinic acid (NA) in the marine environment has caused great harm to human health and the natural environment. Therefore, there is an urgent need to use efficient and economical methods to remove such pollutants from the environment. In this study, a nicotine and NA-degrading bacterium\u2014strain JQ581\u2014was isolated from sediment from the Ea"], "cc_re__cc_re__cc_by_4_0": ["and (D) indicate the size and direction of each gene. NMM: N-Methylmyosmine; SAP: 3-Succinoylsemialdehyde-pyridine; FA: Fumaric acid; MA: Maleic acid; MAA: Maleamic acid; 25DHP: 2,5-Dihydroxypyridine. Numbers within the arrows indicate the DNA sequence identity with the orthologous gene from strain JQ581.&lt;/p&gt;\n                                                            \"></a></div>\n<div class=\"licenseText in-tab\" style=\"padding: 1em 0;\">\nThis is an open access article distributed under the <a href=\"https://creativecommons.org/licenses/by/4.0/\" target=\"_blank\">Creative Commons Attribution License</a> which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).\n</div>\n</div>\n<div id=\"tabs-4\" class=\"ui-tabs-panel\">\n<a id=\"suppl_id\" name=\"supplementary\"></a>\n<h1>Supplementary material</h1>\n<div class=\"in-tab\">\n<ul style=\"margin:0; list-style: none;\">\n<li><a name=\"supplementary_file1\"></a><b>Supplementary File 1:</b>\n<p>\n<a href=\"/1660-3397/15/6/156/s1\">\nSupplementary\n</a>\n(PDF, 39"], "copyright__copyright__copyright": ["ne and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine. Mar. Drugs 2017, 15, 156.\n\nShow more citation formats Show less citations formats\n\nNote that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.\n\nRelated Articles\n\nArticle Metrics\n\nArticle Access Statistics\n\n1\n\nComments\n\n[Return to top]\nMar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert\nBack to Top", "ne and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine. Mar. Drugs 2017, 15, 156.\n\nShow more citation formats Show less citations formats\n\nNote that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.\n\nRelated Articles\n\nArticle Metrics\n\nArticle Access Statistics\n\n1\n\nComments\n\n[Return to top]\nMar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert\nBack to Top", "ne and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine. Mar. Drugs 2017, 15, 156.\n\nShow more citation formats Show less citations formats\n\nNote that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.\n\nRelated Articles\n\nArticle Metrics\n\nArticle Access Statistics\n\n1\n\nComments\n\n[Return to top]\nMar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert\nBack to Top"]}