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dc.contributor.authorMok, Flora SY
dc.contributor.authorThiyagarajan, Vengatesen
dc.contributor.authorQian, Pei-Yuan
dc.date.accessioned2014-11-11T14:30:34Z
dc.date.available2014-11-11T14:30:34Z
dc.date.issued2009-12-17
dc.identifier.citationMok FS, Thiyagarajan V, Qian P-Y (2009) Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa. Proteome Science 7: 44. doi:10.1186/1477-5956-7-44.
dc.identifier.issn1477-5956
dc.identifier.pmid20003395
dc.identifier.doi10.1186/1477-5956-7-44
dc.identifier.urihttp://hdl.handle.net/10754/334579
dc.description.abstractBackground: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle.Results: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase).Conclusions: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms. © 2009 Mok et al; licensee BioMed Central Ltd.
dc.description.sponsorshipWe are grateful to 3 anonymous reviewers for their constructive suggestions in improving the manuscript. We thank Dr. Priscilla Leung and Mr. Jack Man (Genome Research Centre, HKU) for MS analysis. We are also thankful to Dr. Kondethimma H. Chandramouli, Dr. Shawn M. Arellano, Dr. Olivia Ambrogio, and Dr. On On Lee for proof-reading the manuscript. This study was supported by the CAS/SAFEA International Partnership Program for Creative Research Teams, a research grant (GRF #662408) from the HKSAR Government, and KAUST Global Partnership Program awarded to PY Qian.
dc.language.isoen
dc.publisherSpringer Nature
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.subjectcalreticulin
dc.subjectmetalloproteinase
dc.subjectproteome
dc.subjectbarnacle
dc.subjectcomparative study
dc.subjectcontrolled study
dc.subjectjuvenile animal
dc.subjectlarva
dc.subjectlarval development
dc.subjectmarine species
dc.subjectmatrix assisted laser desorption ionization time of flight mass spectrometry
dc.subjectmetamorphosis
dc.subjectnucleotide sequence
dc.subjectPolychaeta
dc.subjectprotein analysis
dc.subjectprotein expression
dc.subjectproteomics
dc.subjectsignal transduction
dc.subjecttwo dimensional gel electrophoresis
dc.subjectupregulation
dc.subjectBalanus amphitrite
dc.subjectInvertebrata
dc.subjectPolychaeta
dc.subjectPseudopolydora
dc.subjectSpionida
dc.subjectThoracica
dc.titleProteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa
dc.typeArticle
dc.identifier.journalProteome Science
dc.identifier.pmcidPMC2803448
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionSwire Institute of Marine Science/School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, China
dc.contributor.institutionCAS/SAFEA International Partnership Program for Creative Research Teams, South China Sea Institute of Oceanology, Chinese Academy of Sciences, China
dc.contributor.institutionKAUST Global Partnership Program, Department of Biology/Atmospheric, Marine and Coastal Environment Program, The Hong Kong University of Science and Technology, Hong Kong SAR, China
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personMok, Flora SY
kaust.personQian, Pei-Yuan
kaust.grant.programKAUST Global Partnership Program
refterms.dateFOA2018-06-14T04:39:11Z
dc.date.published-online2009-12-17
dc.date.published-print2009
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