User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo

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Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor

  • Date: 2012/12/03 10:29 AM EDT
  • Organism: E. coli (K-12 substr.)
  • Strain: Probably D1210, a HB101/K-12 hybrid strain which overexpress the Lacl repressor.


Hypothesis

Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.

Experiment

Material

  • 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]

Expected results

  • Amine protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
  • a heterocyclic base-labile group (NHC?)
  • IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
    • in that case a matching E. coli genotype is "HB101 lacIq, lacY+" (ie laclq)
    • From Sigma, the expected genotype matching the laclq strain is:
      • HB101 F– Δ(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h

References

Personal tools
{"cc_re__cc_re__cc_by_sa_2_5": ["a href=\"/wiki/OpenWetWare:How_to_join\">\n\t\t\t<img style=\"margin:2px;\" \n\t\t\t\tsrc=\"http://openwetware.org/images/0/03/88x31_JoinOWW.png\" border=0 /></a>\n                </div>\n        </div>\n\t\t\t\t\t\t\t</div>\n\n\t\t\t</div><!-- end of the left column -->\n\t\t\n\t\t\n\t\t\t<div class=\"visualClear\"></div>\n\t\t\t<div id=\"footer\">\n\t\t\t\t<div id=\"f-poweredbyico\"><a href=\"http://www.mediawiki.org/\"><img src=\"/skins/common/images/poweredby_mediawiki_88x31.png\" alt=\"Powered by MediaWiki\" /></a></div>\n\t\t\t\t<div id=\"f-copyrightico\"><a href=\"http://creativecommons.org/licenses/by-sa/2.5/\"><img src=\"/somerights20.png\" alt='GNU FDL or Creative Commons BY-SA' /></a></div>\n\t\t\t<ul id=\"f-list\">\n\t\t\t\t<li id=\"copyright\">Content is available under <a href=\"/wiki/Copyright\" title=\"Copyright\">GNU FDL or Creative Commons BY-SA</a>.</li>\n\t\t\t\t<li id=\"privacy\"><a href=\"/wiki/OpenWetWare:Privacy_policy\" title=\"OpenWetWare:Privacy policy\">Privacy policy</a></li>\n\t\t\t\t<li id=\"about\"><a href=\"/wiki/OpenWetWare:About\" title=\"OpenWetWare:About\">About OpenWetWare</a></li>\n\t\t\t\t<li id=\"disclaimer\"><a href"], "copyright__copyright__copyright": ["tidine based autophosphorylation)\n  \u2022 a heterocyclic base-labile group (NHC?)\n  \u2022 IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)\n    \u2022 in that case a matching E. coli genotype is \"HB101 lacIq, lacY+\" (ie laclq)\n    \u2022 From Sigma, the expected genotype matching the laclq strain is:\n      \u2022 HB101 F\u2013 \u0394(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 \u0394(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h\n\nReferences\n\nPersonal tools", "tidine based autophosphorylation)\n  \u2022 a heterocyclic base-labile group (NHC?)\n  \u2022 IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)\n    \u2022 in that case a matching E. coli genotype is \"HB101 lacIq, lacY+\" (ie laclq)\n    \u2022 From Sigma, the expected genotype matching the laclq strain is:\n      \u2022 HB101 F\u2013 \u0394(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 \u0394(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h\n\nReferences\n\nPersonal tools", "tidine based autophosphorylation)\n  \u2022 a heterocyclic base-labile group (NHC?)\n  \u2022 IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)\n    \u2022 in that case a matching E. coli genotype is \"HB101 lacIq, lacY+\" (ie laclq)\n    \u2022 From Sigma, the expected genotype matching the laclq strain is:\n      \u2022 HB101 F\u2013 \u0394(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 \u0394(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h\n\nReferences\n\nPersonal tools", "tidine based autophosphorylation)\n  \u2022 a heterocyclic base-labile group (NHC?)\n  \u2022 IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)\n    \u2022 in that case a matching E. coli genotype is \"HB101 lacIq, lacY+\" (ie laclq)\n    \u2022 From Sigma, the expected genotype matching the laclq strain is:\n      \u2022 HB101 F\u2013 \u0394(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 \u0394(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h\n\nReferences\n\nPersonal tools", "tidine based autophosphorylation)\n  \u2022 a heterocyclic base-labile group (NHC?)\n  \u2022 IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)\n    \u2022 in that case a matching E. coli genotype is \"HB101 lacIq, lacY+\" (ie laclq)\n    \u2022 From Sigma, the expected genotype matching the laclq strain is:\n      \u2022 HB101 F\u2013 \u0394(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 \u0394(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h\n\nReferences\n\nPersonal tools", "tidine based autophosphorylation)\n  \u2022 a heterocyclic base-labile group (NHC?)\n  \u2022 IPTG inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)\n    \u2022 in that case a matching E. coli genotype is \"HB101 lacIq, lacY+\" (ie laclq)\n    \u2022 From Sigma, the expected genotype matching the laclq strain is:\n      \u2022 HB101 F\u2013 \u0394(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 \u0394(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h\n\nReferences\n\nPersonal tools"]}