User:Allison K. Alix/Notebook/Thesis Research/2013/08/12

From OpenWetWare

Jump to: navigation, search
Au@SiO2 DNA attachment Main project page
Previous entry      Next entry

To Do

  • AuNP@SiO2/DNA attachment procedure (10X diluted AuNP@SiO2 prepared with 2uL TES to be used)

AuNP@SiO2/DNA attachment

adapted from Madeleine Y. Bee[1]

1) Prepare a fresh 10X dilution of AuNP@SiO2

  • Sonicate AuNP@SiO2 for 10 minutes
  • Mix 15uL AuNP@SiO2 with 135uL filter milliQ water (@ room temperature), vortex to mix thorooughly

2) Mix 150uL AuNP@SiO2 with 15uL APS and 235uL 100% ethanol. Sonicate for 30 minutes

3) Centrifuge and rinse solution a total of 5 times at 9100rpm for 5 minutes. Redisperse in ethanol for the first three and 10mM sodium phosphate buffer (pH=9) for the last two.

NOTE: after first round of centrifugation, it was apparent 5 minutes was a sufficient amount of time. However, after removal of the supernatant and redispersion in ethanol, there was difficulty getting the particles back into solution. They were allowed to sonicate for 10 minutes to try to induce separation but still looked relatively aggregated. To further induce separate, the sample was subjected to ultrasonic liquid processing for ~15 seconds, which proved to be effective.





Personal tools
{"cc_re__cc_re__cc_by_sa_2_5": ["a href=\"/wiki/OpenWetWare:How_to_join\">\n\t\t\t<img style=\"margin:2px;\" \n\t\t\t\tsrc=\"http://openwetware.org/images/0/03/88x31_JoinOWW.png\" border=0 /></a>\n                </div>\n        </div>\n\t\t\t\t\t\t\t</div>\n\n\t\t\t</div><!-- end of the left column -->\n\t\t\n\t\t\n\t\t\t<div class=\"visualClear\"></div>\n\t\t\t<div id=\"footer\">\n\t\t\t\t<div id=\"f-poweredbyico\"><a href=\"http://www.mediawiki.org/\"><img src=\"/skins/common/images/poweredby_mediawiki_88x31.png\" alt=\"Powered by MediaWiki\" /></a></div>\n\t\t\t\t<div id=\"f-copyrightico\"><a href=\"http://creativecommons.org/licenses/by-sa/2.5/\"><img src=\"/somerights20.png\" alt='GNU FDL or Creative Commons BY-SA' /></a></div>\n\t\t\t<ul id=\"f-list\">\n\t\t\t\t<li id=\"copyright\">Content is available under <a href=\"/wiki/Copyright\" title=\"Copyright\">GNU FDL or Creative Commons BY-SA</a>.</li>\n\t\t\t\t<li id=\"privacy\"><a href=\"/wiki/OpenWetWare:Privacy_policy\" title=\"OpenWetWare:Privacy policy\">Privacy policy</a></li>\n\t\t\t\t<li id=\"about\"><a href=\"/wiki/OpenWetWare:About\" title=\"OpenWetWare:About\">About OpenWetWare</a></li>\n\t\t\t\t<li id=\"disclaimer\"><a href"], "copyright__copyright__copyright": ["NOTE: after first round of centrifugation, it was apparent 5 minutes was a sufficient amount of time. However, after removal of the supernatant and redispersion in ethanol, there was difficulty getting the particles back into solution. They were allowed to sonicate for 10 minutes to try to induce separation but still looked relatively aggregated. To further induce separate, the sample was subjected to ultrasonic liquid processing for ~15 seconds, which proved to be effective.\n\n\n\n\n\nPersonal tools", "NOTE: after first round of centrifugation, it was apparent 5 minutes was a sufficient amount of time. However, after removal of the supernatant and redispersion in ethanol, there was difficulty getting the particles back into solution. They were allowed to sonicate for 10 minutes to try to induce separation but still looked relatively aggregated. To further induce separate, the sample was subjected to ultrasonic liquid processing for ~15 seconds, which proved to be effective.\n\n\n\n\n\nPersonal tools", "NOTE: after first round of centrifugation, it was apparent 5 minutes was a sufficient amount of time. However, after removal of the supernatant and redispersion in ethanol, there was difficulty getting the particles back into solution. They were allowed to sonicate for 10 minutes to try to induce separation but still looked relatively aggregated. To further induce separate, the sample was subjected to ultrasonic liquid processing for ~15 seconds, which proved to be effective.\n\n\n\n\n\nPersonal tools", "NOTE: after first round of centrifugation, it was apparent 5 minutes was a sufficient amount of time. However, after removal of the supernatant and redispersion in ethanol, there was difficulty getting the particles back into solution. They were allowed to sonicate for 10 minutes to try to induce separation but still looked relatively aggregated. To further induce separate, the sample was subjected to ultrasonic liquid processing for ~15 seconds, which proved to be effective.\n\n\n\n\n\nPersonal tools", "NOTE: after first round of centrifugation, it was apparent 5 minutes was a sufficient amount of time. However, after removal of the supernatant and redispersion in ethanol, there was difficulty getting the particles back into solution. They were allowed to sonicate for 10 minutes to try to induce separation but still looked relatively aggregated. To further induce separate, the sample was subjected to ultrasonic liquid processing for ~15 seconds, which proved to be effective.\n\n\n\n\n\nPersonal tools", "NOTE: after first round of centrifugation, it was apparent 5 minutes was a sufficient amount of time. However, after removal of the supernatant and redispersion in ethanol, there was difficulty getting the particles back into solution. They were allowed to sonicate for 10 minutes to try to induce separation but still looked relatively aggregated. To further induce separate, the sample was subjected to ultrasonic liquid processing for ~15 seconds, which proved to be effective.\n\n\n\n\n\nPersonal tools"]}