TGF-β1 (C-509T) Gene Promoter Polymorphism and its Influence on Chronic Pancreatitis

Kavutharapu Sri Manjari, Akka Jyothy, Porika Shravan Kumar, Boddu Prabhakar, Prathibha Nallari, Ananthapur Venkateshwari

Abstract


AIM: Chronic pancreatitis is a long-standing inflammation of the pancreas that alters its normal structure and function. The aim of the study is to investigate the role of promoter polymorphism in the TGF-β1-509 (C/T) gene in chronic pancreatitis. METHODS: A total of 100 chronic pancreatitis patients and 100 control subjects were included in the present study. The genotyping of promoter polymorphism in the TGF-β1-509 (C/T) gene was carried out by tetra-primer ARMS PCR followed by agarose gel electrophoresis. The serum TGF-β1 levels were measured by enzyme-linked immunosorbent assay. Statistical analysis was applied for the significance of the results. RESULTS: The genotype distribution was 57% of C/C, 38% of C/T and 5% of T/T in control subjects, whereas in chronic pancreatitis patients they were 37% of C/C, 39% of C/T, and 24% of T/T genotype respectively. A significant variation in the distribution of T allele was observed in the patient group compared to control subjects [OD = 0.410 (0.261-0.643), p<0.000**]. There was a significantly higher levels of TGF-β1 levels in CP patients compared to healthy subjects. CONCLUSION: The present study found a significant association between the TGF-β1-509 (C/T) gene promoter polymorphism and chronic pancreatitis. Further, the levels were also found to be elevated in patients compared to control subjects. Thus, TGF-β1-509 (C/T) genotype can be considered as one of the risk factor in the etiology of the disease.

Keywords


Chronic inflammation; Pancreatic fibrosis; Extra cellular matrix; Cytokines; Polymorphism

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{"cc_re__cc_re__cc_by_3_0": ["ix; Cytokines; Polymorphism</div>\n\t\t<br />\n\t\t</div>\n\t\n\t\n\t\t\t\t\n\t\t\tFull Text:\n\t\t\t\t\t\t\t\t\t<a target=\"_blank\" href=\"http://www.ghrnet.org/index.php/joghr/article/view/1023/1117\" class=\"file\" target=\"_parent\">PDF</a>\n\t\t\t\t\t\t\t\t\t\t\t<a target=\"_blank\" href=\"http://www.ghrnet.org/index.php/joghr/article/view/1023/1118\" class=\"file\" target=\"_parent\">HTML</a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\n\n<div class=\"separator\"></div>\n\n<h3>Refbacks</h3>\n\n<ul class=\"plain\">\n\t\t\t\t<li>There are currently no refbacks.</li>\n\t</ul>\n\n\n<br /><br />\n\t\t<a target=\"_new\" rel=\"license\" href=\"http://creativecommons.org/licenses/by/3.0/\">\n\t\t\t<img alt=\"Creative Commons License\" style=\"border-width:0\" src=\"http://i.creativecommons.org/l/by/3.0/80x15.png\"/>\n\t\t</a>\n\t\t<br/>\n\t\tThis work is licensed under a <a rel=\"license\" target=\"_new\" href=\"http://creativecommons.org/licenses/by/3.0/\">Creative Commons Attribution 3.0  License</a>.\n\n<span class=\"Z3988\" title=\"ctx_ver=Z39.88-2004&amp;rft_id=http%3A%2F%2Fwww.ghrnet.org%2Findex.php%2Fjoghr%2Farticle%2Fview%2F1023&amp;rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&amp;rft.genre=artic", "p://www.ghrnet.org/index.php/joghr/article/view/1023/1118\" class=\"file\" target=\"_parent\">HTML</a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\n\n<div class=\"separator\"></div>\n\n<h3>Refbacks</h3>\n\n<ul class=\"plain\">\n\t\t\t\t<li>There are currently no refbacks.</li>\n\t</ul>\n\n\n<br /><br />\n\t\t<a target=\"_new\" rel=\"license\" href=\"http://creativecommons.org/licenses/by/3.0/\">\n\t\t\t<img alt=\"Creative Commons License\" style=\"border-width:0\" src=\"http://i.creativecommons.org/l/by/3.0/80x15.png\"/>\n\t\t</a>\n\t\t<br/>\n\t\tThis work is licensed under a <a rel=\"license\" target=\"_new\" href=\"http://creativecommons.org/licenses/by/3.0/\">Creative Commons Attribution 3.0  License</a>.\n\n<span class=\"Z3988\" title=\"ctx_ver=Z39.88-2004&amp;rft_id=http%3A%2F%2Fwww.ghrnet.org%2Findex.php%2Fjoghr%2Farticle%2Fview%2F1023&amp;rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&amp;rft.genre=article&amp;rft.title=Journal+of+Gastroenterology+and+Hepatology+Research&amp;rft.jtitle=Journal+of+Gastroenterology+and+Hepatology+Research&amp;rft.atitle=TGF-%CE%B21+%28C-509T%29+Gene+Promoter+Polymorphism+and+its+Influence+on+Chronic+Pancreatitis&amp"], "copyright__copyright__copyright": [" gene promoter polymorphism and chronic pancreatitis. Further, the levels were also found to be elevated in patients compared to control subjects. Thus, TGF-\u03b21-509 (C/T) genotype can be considered as one of the risk factor in the etiology of the disease.\n\nKeywords\n\n\nChronic inflammation; Pancreatic fibrosis; Extra cellular matrix; Cytokines; Polymorphism\n\nFull Text: PDF HTML\n\nRefbacks\n\n  \u2022 There are currently no refbacks.\n\n\n\nThis work is licensed under a Creative Commons Attribution 3.0 License.", " gene promoter polymorphism and chronic pancreatitis. Further, the levels were also found to be elevated in patients compared to control subjects. Thus, TGF-\u03b21-509 (C/T) genotype can be considered as one of the risk factor in the etiology of the disease.\n\nKeywords\n\n\nChronic inflammation; Pancreatic fibrosis; Extra cellular matrix; Cytokines; Polymorphism\n\nFull Text: PDF HTML\n\nRefbacks\n\n  \u2022 There are currently no refbacks.\n\n\n\nThis work is licensed under a Creative Commons Attribution 3.0 License."]}