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Isolation and structure elucidation of two antioxidant compounds from sudanese Sonchus oleraceous plant

Author: 
Obeid, H. A., Saeed, A. E. M. and Khalid, H. S.
Subject Area: 
Life Sciences
Abstract: 

Sonchus oleraceus is a wild herb that grow after the rainy season in Sudan. Chemical analysis of the herb led to the isolation of two antioxidant compounds. The ethyl acetate extract showed 89% RSA with DPPH (1,1-Diphenyl-2-picrylhydrazyl). 30 gms of the ethyl acetate extract were separated into 12 -fractions with column chromatography. Purification of fraction 1 led to isolation and identification of two compounds. The solvent mixture for fraction one separation was (chloroform: ethyl acetate) 40:60. Purification solvent mixture was (toluene: ethylacetate: formic acid) 9:1:1.The two isolated compounds were a lignan and a chalcone. Their structures were elucidated with NMR (nuclear magnetic resonance) technique.

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