User:Wesley J. Houston/Notebook/MAP/2013/03/07

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3/7/13

  • Prepped plasmids using Zyppy miniprep kit.
  • Digested plasmids with EcoR1 and Pst1.
  • Ran gel electrophoresis to confirm assembly.
Reagent Volume  
15 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20 μL
10x buffer 3.0
EcoR1 1.0
Pst1 1.0
dH2O 5.0 μL
  30 μL --> 37°C/ ~15 min.


  • No DNA present, will repeat ligation.
    • Determined to be a false positive.


Personal tools
{"cc_re__cc_re__cc_by_sa_2_5": ["a href=\"/wiki/OpenWetWare:How_to_join\">\n\t\t\t<img style=\"margin:2px;\" \n\t\t\t\tsrc=\"http://openwetware.org/images/0/03/88x31_JoinOWW.png\" border=0 /></a>\n                </div>\n        </div>\n\t\t\t\t\t\t\t</div>\n\n\t\t\t</div><!-- end of the left column -->\n\t\t\n\t\t\n\t\t\t<div class=\"visualClear\"></div>\n\t\t\t<div id=\"footer\">\n\t\t\t\t<div id=\"f-poweredbyico\"><a href=\"http://www.mediawiki.org/\"><img src=\"/skins/common/images/poweredby_mediawiki_88x31.png\" alt=\"Powered by MediaWiki\" /></a></div>\n\t\t\t\t<div id=\"f-copyrightico\"><a href=\"http://creativecommons.org/licenses/by-sa/2.5/\"><img src=\"/somerights20.png\" alt='GNU FDL or Creative Commons BY-SA' /></a></div>\n\t\t\t<ul id=\"f-list\">\n\t\t\t\t<li id=\"lastmod\"> This page was last modified on 26 March 2013, at 00:01.</li>\n\t\t\t\t<li id=\"viewcount\">This page has been accessed 450 times.</li>\n\t\t\t\t<li id=\"copyright\">Content is available under <a href=\"/wiki/Copyright\" title=\"Copyright\">GNU FDL or Creative Commons BY-SA</a>.</li>\n\t\t\t\t<li id=\"privacy\"><a href=\"/wiki/OpenWetWare:Privacy_policy\" title=\"OpenWetWare:Privacy policy\">Privacy pol"], "copyright__copyright__copyright": ["\n\nFrom OpenWetWare\n\nJump to: navigation, search\nProject name Main project page\nPrevious entry\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Next entry\n\n3/7/13\n\n  \u2022 Prepped plasmids using Zyppy miniprep kit.\n  \u2022 Digested plasmids with EcoR1 and Pst1.\n  \u2022 Ran gel electrophoresis to confirm assembly.\nReagent Volume \u00a0\n15 \u03bcL/lane, 1% agarose; Ladder\nDNA (plasmid) 20 \u03bcL\n10x buffer 3.0\nEcoR1 1.0\nPst1 1.0\ndH2O 5.0 \u03bcL\n\u00a0 30 \u03bcL --> 37\u00b0C/ ~15 min.\n\n\n  \u2022 No DNA present, will repeat ligation.\n    \u2022 Determined to be a false positive.\n\n\nPersonal tools", "\n\nFrom OpenWetWare\n\nJump to: navigation, search\nProject name Main project page\nPrevious entry\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Next entry\n\n3/7/13\n\n  \u2022 Prepped plasmids using Zyppy miniprep kit.\n  \u2022 Digested plasmids with EcoR1 and Pst1.\n  \u2022 Ran gel electrophoresis to confirm assembly.\nReagent Volume \u00a0\n15 \u03bcL/lane, 1% agarose; Ladder\nDNA (plasmid) 20 \u03bcL\n10x buffer 3.0\nEcoR1 1.0\nPst1 1.0\ndH2O 5.0 \u03bcL\n\u00a0 30 \u03bcL --> 37\u00b0C/ ~15 min.\n\n\n  \u2022 No DNA present, will repeat ligation.\n    \u2022 Determined to be a false positive.\n\n\nPersonal tools", "\n\nFrom OpenWetWare\n\nJump to: navigation, search\nProject name Main project page\nPrevious entry\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Next entry\n\n3/7/13\n\n  \u2022 Prepped plasmids using Zyppy miniprep kit.\n  \u2022 Digested plasmids with EcoR1 and Pst1.\n  \u2022 Ran gel electrophoresis to confirm assembly.\nReagent Volume \u00a0\n15 \u03bcL/lane, 1% agarose; Ladder\nDNA (plasmid) 20 \u03bcL\n10x buffer 3.0\nEcoR1 1.0\nPst1 1.0\ndH2O 5.0 \u03bcL\n\u00a0 30 \u03bcL --> 37\u00b0C/ ~15 min.\n\n\n  \u2022 No DNA present, will repeat ligation.\n    \u2022 Determined to be a false positive.\n\n\nPersonal tools", "\n\nFrom OpenWetWare\n\nJump to: navigation, search\nProject name Main project page\nPrevious entry\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Next entry\n\n3/7/13\n\n  \u2022 Prepped plasmids using Zyppy miniprep kit.\n  \u2022 Digested plasmids with EcoR1 and Pst1.\n  \u2022 Ran gel electrophoresis to confirm assembly.\nReagent Volume \u00a0\n15 \u03bcL/lane, 1% agarose; Ladder\nDNA (plasmid) 20 \u03bcL\n10x buffer 3.0\nEcoR1 1.0\nPst1 1.0\ndH2O 5.0 \u03bcL\n\u00a0 30 \u03bcL --> 37\u00b0C/ ~15 min.\n\n\n  \u2022 No DNA present, will repeat ligation.\n    \u2022 Determined to be a false positive.\n\n\nPersonal tools", "\n\nFrom OpenWetWare\n\nJump to: navigation, search\nProject name Main project page\nPrevious entry\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Next entry\n\n3/7/13\n\n  \u2022 Prepped plasmids using Zyppy miniprep kit.\n  \u2022 Digested plasmids with EcoR1 and Pst1.\n  \u2022 Ran gel electrophoresis to confirm assembly.\nReagent Volume \u00a0\n15 \u03bcL/lane, 1% agarose; Ladder\nDNA (plasmid) 20 \u03bcL\n10x buffer 3.0\nEcoR1 1.0\nPst1 1.0\ndH2O 5.0 \u03bcL\n\u00a0 30 \u03bcL --> 37\u00b0C/ ~15 min.\n\n\n  \u2022 No DNA present, will repeat ligation.\n    \u2022 Determined to be a false positive.\n\n\nPersonal tools", "\n\nFrom OpenWetWare\n\nJump to: navigation, search\nProject name Main project page\nPrevious entry\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Next entry\n\n3/7/13\n\n  \u2022 Prepped plasmids using Zyppy miniprep kit.\n  \u2022 Digested plasmids with EcoR1 and Pst1.\n  \u2022 Ran gel electrophoresis to confirm assembly.\nReagent Volume \u00a0\n15 \u03bcL/lane, 1% agarose; Ladder\nDNA (plasmid) 20 \u03bcL\n10x buffer 3.0\nEcoR1 1.0\nPst1 1.0\ndH2O 5.0 \u03bcL\n\u00a0 30 \u03bcL --> 37\u00b0C/ ~15 min.\n\n\n  \u2022 No DNA present, will repeat ligation.\n    \u2022 Determined to be a false positive.\n\n\nPersonal tools"]}