The Human Tumor Dissociation Kit (Miltenyi Biotec 130095929) was used per the
manufacturer’s protocol to generate a single-cell suspension from the tumor sample. After

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dissociation, cells were cultured in DMEM (Sigma D6546) supplemented with 10% fetal bovine
serum, 5 mL Glutamax (2 mM, ThermoFisher 35050061), and 5 mL Penicillin/Streptomycin (100
U/mL and 100 μg/mL, respectively, ThermoFisher 15140122) at 37°C, 5% CO2, and 21% O2.
Cells were cultured in adherent culture dishes and passaged 1-2 times per week with 0.25%
trypsin.

### Language ###
English

### Assessment ###
Query about cell culture temperature conditions, which is a specific technical detail provided in the text.

### Query ###
What temperature are cancer cells typically grown at in laboratory conditions?
What temperature are cancer cells typically grown at in laboratory conditions?