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Our group has helped to launch research on RNA processing in plants, following our discovery that animal pre-mRNAs failed to be processed in plant cell. Consequently, we have focused on the characterisation of SR proteins which are critical for splice selection and spliceosome assembly.
Using several methods of defining protein-protein interactions we have established networks of interacting splicing factors. In addition, we have started to characterise splicing factors, which are important for the regulation of spliceosome assembly, like cyclophilins and SR specific kinases.
Due to various gene duplication events plant have twice as much SR proteins as vertebrates, however, to date we have isolated and characterised most of them. We have shown for some of these SR proteins that deregulation has an impact on alternative splicing of some genes and consequently influence plant development.
Intriguingly, many of the SR proteins are themselves alternatively spliced and their splicing pattern is influenced by developmental and environmental conditions. Recently, we have uncovered alternative splicing events in the plant specifc SR protein subfamilies, which is evolutionary highly conserved from geen algae to flowering plants.
Key lab techniques: protein-protein interactions, RNA structure analysis, confocal microscopy, Arabidopsis transformation, plant tissue culture, gene expression analysis in plants, in vitro splicing assays.
Key lab reagents: GFP-, YFP- clones for plant SR proteins and subnuclear studies, Arabidopsis SR cDNAs, plant expression vectors.
Lab contact: Dr Maria Kalyna: maria.kalyna@univie.ac.at
Lab website:
Learn more about alternative splicing
What is alternative splicing?
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Full Professor, University of Bern
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