The citizens expelled it in 1552, allying with France: this was unacceptable for Charles, who sent his general Gian Giacomo Medici to lay siege to it with a Florentine-Imperial army.
The citizens removed it in 1552, allying with France: Charles didn't agree to that.  He than sent his general Gian Giacomo Medici to capture it with a Florentine-Imperial army.