has it been referred to in any movies as well?
Yes - Viridiana, MASH, Jesus Christ Superstar
Film and television
In Luis Buñuel's 1961 Spanish film "Viridiana", a "tableau vivant" of Leonardo's painting is staged with beggars. Another beggar "photographs" the scene by flashing them. This scene contributed to its refusal by the Vatican who called it "blasphemous." The painting is parodied in the original 1970 movie "MASH", in the scene where Hawkeye Pierce stages a "last supper" for Walt (Painless) Waldowski before his planned suicide. The various doctors, all in surgical scrubs, pose in imitation of the painting. In Norman Jewison's 1973 film version of "Jesus Christ Superstar", in the Last Supper sequence, Jesus and the 12 apostles briefly mimic the posture of each character in Da Vinci's painting. The painting is also parodied in Mel Brooks's 1981 movie "History of the World, Part I", making Leonardo a contemporary of Jesus.

when was the first man made diamond made 1954 what are the materials required to make this? Pure carbon, the belt press, the cubic press and the split-sphere (BARS) press. what temperature-pressure conditions are required fopr this? High pressure, high temperature. can you mention any of its uses? Machine tools and cutting tools; as a Thermal conductor; as an Optical material; semiconductors ; gemstones what is the first one? A machine for handling or machining metal or other rigid materials, usually by cutting, boring, grinding, shearing, or other forms of deformation can you provide me the historical background for such tools? Forerunners of machine tools included bow drills and potter's wheels, which had existed in ancient Egypt prior to 2500 BC, and lathes, known to have existed in multiple regions of Europe since at least 1000 to 500 BC. can you name any prominent figure involved in this background? Leonardo da Vinci where was he born? Tuscan hill town of Vinci, Republic of Florence. what was his profession? Painter, engineer can you name any such painted work by him? Mona Lisa, The Last Supper by whom was the second one commissioned? Ludovico Sforza - Duke of Milan what media was used for this work? Oil painting did it needed repairs later? Yes -  Between 1946 and 1954, undertook a clean-and-stabilise restoration, which Brera director was involved in. Pellicioli reattached paint to the wall using a clear shellac, making it relatively darker and more colorful, and removed some of the overpainting. However, as of 1972, the repainting done in various restorations had made the heads of saints Peter, Andrew, and James differ significantly from the original design. what is the cultural significance of this painted piece? It has frequently been referenced, reproduced, or parodied in Western culture.
Yes, Viridiana Yes,  "Viridiana" Yes. Viridiana, MASH and Jesus Christ Superstar
The Last Supper (Leonardo) The Last Supper (Leonardo) The Last Supper (Leonardo)
Film and television Film and television Film and television
In Luis Buñuel's 1961 Spanish film "Viridiana", a "tableau vivant" of Leonardo's painting is staged with beggars In Luis Buñuel's 1961 Spanish film "Viridiana", a "tableau vivant" of Leonardo's painting is staged with beggars.  In Luis Buñuel's 1961 Spanish film "Viridiana", a "tableau vivant" of Leonardo's painting is staged with beggars. Another beggar "photographs" the scene by flashing them. This scene contributed to its refusal by the Vatican who called it "blasphemous." The painting is parodied in the original 1970 movie "MASH", in the scene where Hawkeye Pierce stages a "last supper" for Walt (Painless) Waldowski before his planned suicide. The various doctors, all in surgical scrubs, pose in imitation of the painting. In Norman Jewison's 1973 film version of "Jesus Christ Superstar", in the Last Supper sequence, Jesus and the 12 apostles briefly mimic the posture of each character in Da Vinci's painting. 
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The Last Supper (Leonardo) [SEP] Film and television
In Luis Buñuel's 1961 Spanish film "Viridiana", a "tableau vivant" of Leonardo's painting is staged with beggars. Another beggar "photographs" the scene by flashing them. This scene contributed to its refusal by the Vatican who called it "blasphemous." The painting is parodied in the original 1970 movie "MASH", in the scene where Hawkeye Pierce stages a "last supper" for Walt (Painless) Waldowski before his planned suicide. The various doctors, all in surgical scrubs, pose in imitation of the painting. In Norman Jewison's 1973 film version of "Jesus Christ Superstar", in the Last Supper sequence, Jesus and the 12 apostles briefly mimic the posture of each character in Da Vinci's painting.