in ancient athenian drama who played the roles of all the female characters
Women were excluded from participating in drama
Women in Classical Athens
women may have been excluded. 
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Women in Classical Athens [SEP] Theatre
Other evidence of the presence of women at the theatre in Athens includes the absence of surviving prohibitions against their attendance and the importance of women in Athenian rituals, especially those associated with Dionysus. According to Simon Goldhill, the evidence is fundamentally inconclusive. Goldhill writes that the theatre can be seen as a social and political event analogous with the Assembly and the courtroom, and women may have been excluded. David Kawalko Roselli writes that although Goldhill's perspective is valuable, he does not sufficiently consider the theatre's ritual purpose. If women did attend the theatre, they may have sat separately from the men.