what did the physiologist start his career as?
He was put in charge of the chemical department of the pathological laboratory at Berlin, under Rudolf Virchow
Wilhelm Kühne
At the end of 1863 he was put in charge of the chemical department of the pathological laboratory at Berlin, under Rudolf Virchow; 
what is an enzyme? Enzymes are Proteins that act as biological catalysts who discovered it? Anselme Payen was he the same person who gave the name? No, German physiologist Wilhelm Kühne first used the term "enzyme" name some factors that affect it's activity? Temperature, pH, substrate concentration what functions do they perform? Enzymes are known to catalyze more than 5,000 biochemical reaction types
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Wilhelm Kühne [SEP] Biography
Kühne was born at Hamburg on 28 March 1837. After attending the gymnasium in Lüneburg, he went to Göttingen, where his master in chemistry was Friedrich Wöhler and in physiology Rudolph Wagner. Having graduated in 1856, he studied under various famous physiologists, including Emil du Bois-Reymond at Berlin, Claude Bernard in Paris, and KFW Ludwig and EW von Brücke in Vienna. At the end of 1863 he was put in charge of the chemical department of the pathological laboratory at Berlin, under Rudolf Virchow; in 1868 he was appointed professor of physiology at Amsterdam; and in 1871 he was chosen to succeed Hermann von Helmholtz in the same capacity at Heidelberg, where he died on 10 June 1900.