what is the limitations of the first application of the technology that you mentioned?
It can only be performed if the sample is in crystal form; As the crystal's repeating unit cell, becomes larger and more complex, the atomic-level picture provided by X-ray crystallography becomes more fuzzy
X-ray crystallography
As the crystal's repeating unit, its unit cell, becomes larger and more complex, the atomic-level picture provided by X-ray crystallography becomes less well-resolved (more "fuzzy") for a given number of observed reflections. Two limiting cases of X-ray crystallography—"small-molecule" (which includes continuous inorganic solids) and "macromolecular" crystallography—are often discerned. "Small-molecule crystallography" typically involves crystals with fewer than 100 atoms in their asymmetric unit; such crystal structures are usually so well resolved that the atoms can be discerned as isolated "blobs" of electron density. By contrast, "macromolecular crystallography" often involves tens of thousands of atoms in the unit cell. Such crystal structures are generally less well-resolved (more "smeared out"); the atoms and chemical bonds appear as tubes of electron density, rather than as isolated atoms. In general, small molecules are also easier to crystallize than macromolecules; however, 
who is robert dirks? An American chemist known for his theoretical and experimental work in DNA nanotechnology. what is the full form of dna? Deoxyribonucleic acid what are the applications of the last technology you mentioned? X-ray crystallography, nuclear magnetic resonance spectroscopy of proteins what are its different types of designs? Structural design, Sequence design what can you tell me about the first type? Representation by a specific arrangement of nucleic acid strands. and the second type? Nucleic acid design is the process of assigning a specific nucleic acid base sequence to each of a structure's constituent strands so that they will associate into a desired conformation. did the chemist have a nickname? UNANSWERABLE where did he study? Wabash College when did he die? February 3, 2015
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X-ray crystallography [SEP] Limitations
As the crystal's repeating unit, its unit cell, becomes larger and more complex, the atomic-level picture provided by X-ray crystallography becomes less well-resolved (more "fuzzy") for a given number of observed reflections. Two limiting cases of X-ray crystallography—"small-molecule" (which includes continuous inorganic solids) and "macromolecular" crystallography—are often discerned. "Small-molecule crystallography" typically involves crystals with fewer than 100 atoms in their asymmetric unit; such crystal structures are usually so well resolved that the atoms can be discerned as isolated "blobs" of electron density. By contrast, "macromolecular crystallography" often involves tens of thousands of atoms in the unit cell. Such crystal structures are generally less well-resolved (more "smeared out"); the atoms and chemical bonds appear as tubes of electron density, rather than as isolated atoms.