h amp e stained sections of the fdp tendons after decellularization and oxidation no nuclear material was evident via h amp e staining dapi staining revealed the presence of dna and rna within the decellularized oxidized tendon scaffolds fig2 however this material was neither organized nor condensed in appearance as seen in the untreated tendons an increase in intra fascicular and inter fascicular space after treatment was also observed via h amp e staining c scanning electron microscopy of fresh frozen fdp tendons and decellularized oxidized fdp tendon scaffolds scanning electron microscopy confirmed the dense micro architecture observed in histologic sections of fresh frozen fdp tendon as well as an increase in pore size and porosity following treatment fig3 scanning electron microscopy was performed on fresh frozen fdp tendons and decellularized oxidized fdp tendon scaffolds specimens of the fresh frozen fdp tendon and the fdp tendon scaffold were removed directly from storage at u2212 80 u00b0 c equilibrated in dnase free rnase free water at 37 u00b0 c for 30 minutes patted dry and mounted for analysis cross sectional electronmicrographs were obtained at 25 0 kv 50 pa 50 u00d7 magnification using a hitachi 5 2600 scanning electron microscope hitachi high technologies america pleasanton calif d determination of dna content in fresh frozen fdp tendons and.