in sterile polyethylene bags 3000 cc o 2 at 22 8 u00b0 c m 2 24 hr at 1 atm and maintained at 3 u00b0 c within 1 h of collection the carcasses were sampled on the day of collection by rinsing in 100 ml of sterile deionized water according to the procedure described in cox et al 1981 poultry sci 60 768 770 a stock solution was made by dissolving 2 5 g of 2 4 4 39 trichloro 2 39 hydroxydiphenyl ether irgasan dp300 gas 33 80345 ciba geigy greensboro n c in 100 ml of 100 ethanol an aliquot of the stock solution 0 1 ml was then added to 100 ml of brain heart infusion broth bhi difco laboratories detroit mich to achieve a final concentration of 25 u03bcg irgasan ml in the bhi irgasan bhi i medium one milliliter of rinse fluid from each carcass was placed into 9 ml of bhi i samples 1 ml were assayed in duplicate in bactometer module wells capacitance assays were conducted at 25 u00b0 c using test code 6 on the bactometer microbial monitoring system m128 biomerieux vitek inc hazelwood mo as soon as capacitance detection times dt were recorded 1 ml of the sample was immediately taken from the module well placed into 9 ml of sterile bacto peptone difco laboratories detroit mich diluted to 10 6 10 7 or 10 8 spread onto duplicate plate count agar pca difco laboratories detroit mich plates and incubated at.