and polymerization initiator such as potassium persulfate the free acrylic groups attached to the capillary wall serve as anchors for growing linear polyacrylamide lpa chains the major problem of this coating is that the lpa molecules cannot cover the capillary wall completely all the above coating schemes may be used for this invention in one embodiment the coating materials are selected from linear polyacrylamide polymethylacrylamide poly dimethylacrylamide cross linked polyacrylamide polyvinyl alcohol poly ethylene oxide hydroxy ethyl cellulose hydroxypropyl cellulose and their derivatives in one embodiment the oxygen in the monomer solution needs to be removed since it is a radical suppression reagent maintaining a constant concentration preferably a low concentration of oxygen is a key to prepare rcpas and linear polyacrylamides as well reproducibly the degassing process is performed before polymerization reaction is initiated after an rcpa is prepared it is loaded into a separation column a sample is then introduced into the separation column a voltage is applied across the separation column and the separated analytes are detected on or off the column by a detection scheme one aspect of the present invention uses a tubular column filled with a replaceable cross linked polyacrylamide for molecular separations in a specific embodiment the separation column is a fused silica capillary a microchannel in a microchip device or a large diameter column for laboratory and industry scale preparative separations multiplexed capillary electrophoresis has been employed to boost the analysis throughput the invented apparatus and methods can be.