or m in human body fluid utilizing immuoglobulins chemically coupled to latex carrier particles as the reagent application_number US 73421676 A description the following will serve to illustrate the invention in more detail a 100 mg portion of immunoglobulin preparation containing iga igm and igg in 10 ml of 0 01m phosphate or glycine 0 15m sodium chloride ph 8 2 is mixed with 2 ml of 10 latex dow diagnostics average particle size 0 79 u03bc and 2 ml of a solution of 40 mg ml of 1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride in water the mixture is heated in a water bath at 5 u00b0 55 u00b0 c for 10 minutes the reaction mixture is cooled centrifuged and washed three times with 0 01m phosphate 0 15m sodium chloride 0 02 sodium azide ph 7 0 buffer the immunoglobulin latex reagent is finally suspended in the phosphate saline azide buffer containing 0 02 sodium taurocholate and 0 02 triton u00ae x 100 in its qualitative aspect the test is performed on a glass slide which is made up with 3 to 6 depressed ovals built in in the first step a drop of a dilution of the sample to be tested e g adult human serum cord serum spinal cord fluid etc is placed in each oval along with a drop of antiserum to for example human igm the antisera to igg iga or igm are produced in rabbits or goats by standard procedures known in the art and are prediluted appropriately for the test the antiserum and sample are mixed with an applicator straw and swirled together for.