5467 with modifications after zimmermann et al 1990 nucleic acids research 18 1067 the u201c rr drhodamin terminator cycle sequencing kit u201d from pe applied biosystems product no 403044 weiterstadt germany was used separation by gel electrophoresis and analysis of the sequencing reaction was carried out in a u201c rotiphoresis nf acrylamide bisacrylamide u201d gel 29 1 product no a124 1 roth karlsruhe germany with the u201c abi prism 377 u201d sequencing device from pe applied biosystems weiterstadt germany the raw sequence data obtained were then processed using the staden program package 1986 nucleic acids research 14 217 231 version 97 0 the individual sequences of the pzero1 derivatives were assembled to a coherent contig the computer controlled coding region analysis was prepared with the program xnip staden 1986 nucleic acids research 14 217 231 further analyses were carried out with the u201c blast search programs u201d altschul et al 1997 nucleic acids research 25 3389 3402 against the non redundant data base of the u201c national center for biotechnology information u201d ncbi bethesda md usa the nucleotide sequence obtained is shown in seq id no 1 the analysis of the nucleotide sequence revealed an open reading frame of 267 base pairs which was designated the ptsh gene the ptsh gene codes for a protein of 89 amino acids preparation of a shuttle vector pec k18mob2ptshexp in order to enhance the ptsh gene in c glutamicum chromosomal dna was isolated from the atcc 13032 strain according to the method of eikmanns et al microbiology 140 1817 1828 1994 on the basis of the sequence of the ptsh gene known from example 2 for c glutamicum the following oligonucleotides were selected for the polymerase chain reaction ptshexpl 5 u2032 acc act ggt gca atc tcc at 3 u2032 ptshexp2 5.