known to work optimally at this temperature any enzyme can be used without restrictions some examples of heat resistant hydrolases are papain protease s brand name proleather brand name thermoase brand name alcalase brand name and protin a brand name in this method a heat resistant hydrolase that has about 10 or more residual activity after heating for 30 min at 80 u00b0 c is desired in addition use of multiple enzymes is more effective the preferred reaction time is 30 min 10 h at last the reaction solution needs to be heated to inactivate the hydrolase the enzyme that was used in this method can be inactivated by heating the reaction solution for 10 s or more at 100 u00b0 c and above the reaction solution collected as mentioned above was centrifuged and the supernatant was collected the supernatant is dried to obtain the powdered whey protein hydrolysate product the precipitate formed during centrifugation has less effect on decreasing allergic reactions compared with that of the supernatant and this should be removed however the reaction solution can be dried and used as it is the whey protein hydrolysate which can be obtained by this method is quantitated by inhibition elisa japanese journal of pediatric allergy 1 36 1987 its antigenicity was confirmed to be 1 10 000 or less compared with that of u03b2 lactoglobulin and u2267 1 10 000 of whey protein and is extremely.