using the present method at a dose rate of approximately 0 7 kgy hr none of the protein was insoluble this would indicate that little or no change or degradation of the protein occurred further gergely et al found that a liquid formulation of human igg lost all of its activity after irradiation in studies using the present method on intravenous immunoglobulin ivig in liquid form it was shown that greater than 70 of a specific antibody in hyperimmune ivig was retained in this experiment alpha 1 proteinase inhibitor and fibrinogen were irradiated in lyophilized form the samples were placed in a gammacell 220 and irradiated according to the present process to a total dose of 25 kgy samples were then returned to the laboratory for analysis the dose rate was 0 72 kgy hr the alpha 1 proteinase inhibitor both treated and control were 40 of a standard normal pooled plasma sample the mancini radial immunodiffusion technique was used as the assay the topical fibrinogen complex vials were reconstituted in 10 ml of water protamine sulphate at a concentration 10 mg ml was added to the samples there was instant formation of monomer in all three preparations in this experiment factors vii viii and ix were irradiated in lyophilized form the samples were placed in a gammacell 220 and irradiated to various total doses at a dose rate of approximately 1 kgy hr factor vii retained 67 activity at 20 kgy and 75 at 10 kgy factor viii retained 77 activity at 20 kgy and 88 at 10 kgy similarly factor ix showed an activity level of 70 at 20 kgy and 80 at 10 kgy.