at least 3 0 or at least 4 0 style 400 cotton is cleaned by deionized water at 95 u00b0 c and is cut in swatches of 9 u00d7 9 cm 50 u03bcl of lard sudan red 0 75 mg dye g of lard is applied to the center of each swatch and the soiled swatches are heat treated at 70 u00b0 c for 25 minutes and cured overnight 7 soiled swatches are washed for 20 minutes at 30 u00b0 c in a terg o tometer test washing machine in 1000 ml of wash liquor with 4 g l of test detergent in water with hardness of 15 u00b0 dh ca 2 mg 2 4 1 followed by 15 minutes rinsing in tap water and drying overnight the lipase is added to the wash liquor at a dosage of 0 25 mg enzyme protein per liter a control is made without addition of lipase variant the soil removal is evaluated by measuring the remission at 460 nm after the first washing cycle and the results are expressed as u03b4r by subtracting the remission of a blank washed at the same conditions without lipase the test detergent used in this specification has the following composition in by.