was dissolved in a small amount of a 20 mm hydrochloric acid solution the mixture was loaded onto cosmosil u00ae 5c18 column trifluoroacetic acid solution in which the concentration of acetonitril was altered was added to the column to elute tachyplesin iii tachyplesin iii was dissolved in a 4 mannitol solution to a final concentration of 2 mg ml 0 5 ml of the resulting solution was combined with 0 5 ml of a horseshoe crab hemocyte lysate to the mixture 0 2 ml of a 5 mm boc leu gly arg pna solution was added the resulting mixture was lyophilized to give a desired reagent the sensitivity of u03b2 glucan detection reagents of the present invention to endotoxin nippon pharmacopeia standard products and u03b2 glucan was tested as follows the u03b2 glucans reagent was dissolved in 2 1 ml of 40 mm tris hcl buffer ph 7 2 and the solution was dispensed 0 1 ml each to sterilized tubes 0 1 ml of increased concentrations of endotoxins and u03b2 glucans was added to the tube the tube was vortexed and incubated at 37 u00b1 1 u00b0 c for 30 u00b1 1 minutes after incubation 0 4 ml of 0 4n acetic.