these and stored in 37 u00b0 c 5 carbon dioxide concentration next morning the culture medium was exchanged to dmem including 1 phosphate buffered saline the purified water extract the 50 ethyl alcohol solution extract and the ethyl alcohol extract of pueraria mirifica obtained by reference example 1 were added to the culture mediums so the final concentration as to be 0 a contrast 1 10 and 100 u03bcg ml and mixed the culture mediums were exchanged after the incubation of 4 days and the solutions same to the first were added next morning the culture mediums were eliminated the cell was retrieved after washing with phosphate buffer solution the cell number of fibroblast nb1rgb grown in the each extracts was evaluated as a cell growth rate in comparison with the contrast as a comparative example soybean extract which has already been known as a cell activator was used and same test was carried out table 6 shows the result the cell numbers in the extracts obtained by reference example 1 were compared with the cell number of the comparative example soybean extract and also contrast additive free the cell activation effect was evaluated as cell growth rate also the cell number counted by using an erythrocytometer it was clearly understood from table 6 that the extracts of pueraria mirifica have high cell activation ability to fibroblast.