affect bacterial cell membrane permeability fig1 illustrates the affect of several compounds of formula i on the permeability of bacterial cells support solution support mic mic compound purity a u03bcm b u03bcm c f19 82 lt 3 125 3 1 u00b1 0 2 f17 87 lt 3 125 3 5 u00b1 0 5 b19 80 lt 3 125 4 0 u00b1 0 5 f5 97 12 5 25 17 u00b1 1 0 b18 87 25 50 54 u00b1 1 0 linezolid u2014 u2014 5 0 u00b1 1 0 ciprofloxacin u2014 u2014 0 6 u00b1 0 2 table 1 antibacterial activity of lead compounds from rink support a determined by hplc trace at 254 nm b solution phase assay from cleaved macroarray c authentic solution phase sample chalcones b19 f17 and f19 had antibacterial activity with mics of 4 0 u00b1 0 5 u03bcm 3 5 u00b1 0 5 u03bcm and 3 1 u00b1 0 2 u03bcm respectively table 1 note that compound names are based on the letter and number code of fig4 which identifies the acetophenone and benzaldehyde used to form the base chalcone several active chalcones b19 f17 f19 f5 and b18 were synthesized in solution to obtain more precise mic values using our previously described solution phase assay.