for c 22 h 32 n 4 o 6 f 3 c 2 o 2 h h 2 o c 50 44 h 5 95 n 9 80 found c 50 33 h 6 02 n 9 67 the platelet binding for activity of the compounds of the present invention can be demonstrated by the assay described below healthy male or female dogs were fasted for 8 hours prior to drawing blood then 30 ml whole blood was collected using a butterfly needle and 30 cc plastic syringe with 3 ml of 0 129m buffered sodium citrate 3 8 the syringe was rotated carefully as blood was drawn to mix the citrate platelet rich plasma prp was prepared by centrifugation at 975 u00d7 g for 3 17 minutes at room temperature allowing the centrifuge to coast to a stop without braking the prp was removed from the blood with a plastic pipette and placed in a plastic capped 50 ml corning conical sterile centrifuge tube which was held at room temperature platelet poor plasma ppp was prepared by centrifuging the remaining blood at 2000 u00d7 g for 15 minutes at room temperature allowing the centrifuge to coast to a stop without braking the prp was adjusted with ppp to a count of 2 3 u00d7 10 8 platelets per ml 400 ul of the prp preparation and 50 ul of the compound to be tested in solution or saline were preincubated for 1 minute at 37 u00b0 c in an aggregometer biodata horsham pa.