16 mmol in hexane 60 ml were added thionyl chloride 2 5 g 21 mmol and n n dimethylformamide 0 5 ml the reaction mixture was heated to reflux for 1 h after the reaction was complete the solvent was evaporated under reduced pressure to yield the desired 2 isopropoxy 6 pentadecylbenzoyl chloride which was redissolved in dichloromethane 50 ml and used for condensation with following anilides to yield respective benzamides purification of human core histones and recombinant proteins u2014 human core histones were purified from hela nuclear pellet as described previously 13 the flag epitope tagged human topoisomerase i histone deacetylase 1 hdac1 and pcaf were purified from the recombinant baculovirus infected insect cell line sf21 by the immunoaffinity purification using m2 agarose sigma 14 full length p300 was also purified from the recombinant baculovirus infected sf21 cells as a his 6 tagged protein through the ni nta affinity column qiagen as described previously 13 the his 6 tagged nucleosome assembly protein 1 nap1 used for the in vitro chromatin assembly was purified from e coli cells as previously reported 13 and the flag tagged chimeric activator ga14 vp16 expressed in e coli and purified by immunoaffinity purification with m2 agarose human positive transcriptional coactivator pc4 was expressed in e coli and purified as described.