first dispensing sample and reagent the second a delay cycle the third and fourth initial and final absorbance readings respectively the difference between the initial and final absorbance readings is used to compute the sample concentration using the standard curve provided 7 remove the multicurvette and rinse with water leave to soak overnight in water flush the pipette with 34 contrad 34 cleaning solution by activating the wash cycle and repeat the wash procedure with water 8 the normal range is 1 14 to 1 96 mmol l diabetic specimens will usually be greater than 2 0 mmol l 1 the aba 200 will automatically reject incorrect rx type rx direction temperature revolution and time but not incorrect filters or sample reagent volumes settings 2 colour reagent with sodium carbonate bicarbonate buffer is stable if stored at 4 u00b0 c for up to 1 week check the ph immediately prior to use and ensure that it is 10 55 to 10 60 ensure that all nbt is dissolved before using the colour reagent 3 control specimens should give values within the 2 u00d7 sd range in accordance with preferred quality control charts it is preferable to document all assays where excursion.