were returned to room air with normal oxygen content on p12 olanzapine in water or veh water was administered once daily by gavage beginning on p10 and continuing through p16 retinal neovascularization was evaluated on p17 after 5 days of exposure of the animals to room air retinal neovascularization was evaluated by angiography in mice subjected to oir as described previously smith l e wesolowski e mclellan a et al oxygen induced retinopathy in the mouse invest ophthalmol vis sci 1994 35 1 101 111 p17 mice were deeply anesthetized and then were perfused through the left ventricle with 1 ml of pbs containing 50 mg of high molecular weight 2000 kda fluorescein dextran sigma st louis mo eyes were enucleated and fixed in 4 paraformaldehyde for 24 hours after removal of the lens the retina was dissected and wholemounted with glycerol gelatin quantification of retinal neovascularization was performed as described previously chen j connor k m aderman c m smith l e erythropoietin deficiency decreases vascular stability in mice j clin invest 2008 118 2 526 533 images of retinal whole mounts taken at 4 u00d7 magnification on an epifluorescence microscope olympus center valley pa were imported into adobe photoshop 7 0 software adobe systems mountain view calif and merged to produce an image of the entire retina neovascularization was quantified as described previously bai y ma j x guo j et al m u00fcller cell derived vegf is a significant contributor to retinal neovascularization j pathol 2009 219 4 446 454 the photoshop freehand tool was used to outline areas of neovascular tuft formation and the area of neovascularization in pixels was expressed as a percentage of the area of the whole retina inpixels to avoid bias quantification of neovascularization was performed by.