final cell free ribavirin concentration and conversion are shown in table ii as a function of the initial guanosine concentration in the cell free solution the increase in the final conversion obtained by extracting ribavirin from the cells is shown in table iii table ii______________________________________ribavirin production as a function of initialguanosine and 1 2 4 triazole 3 carboxamideconcentrations between 100 and 200 mm initial rateinitial of ribavirin ribaviringuanosine production conc g l conversionconcen mm g l hr 7 hr final 7 hr final______________________________________100 3 9 14 8 16 8 61 70125 4 5 18 8 20 9 62 70150 5 4 22 7 25 1 62 70175 5 4 25 7 28 9 60 68200 5 5 28 3 32 8 58 68______________________________________ table iii______________________________________impact of extraction of ribavirin fromcells on the final conversioninitial final conversion guanosine no one twoconcen mm extraction extraction extractions______________________________________100 70 78 79125 70 79 80150 70 79 80175 68 77 78200 68 77 79______________________________________ bioconversion media were prepared as in example 1 except the initial amounts of guanosine and 1 2 4 triazole 3 carboxamide were 40 millimoles the initial ph of each flask was adjusted to ph 7 2 with potassium hydroxide the bioconversions were initiated as in example 1 the cells were added promptly as the temperature reached 70 u00b0 c to avoid gelling of the bioconversion mixture forty millimole.