all the flow through fraction in eppendorf tubes until level is at resin illustrative of the various buffering compositions useful in this technique are sample running buffers including but not limited to bicine buffers of various molarities ph 39 s nacl content bis tris buffers of various molarities ph 39 s nacl content diethanolamine of various molarities ph 39 s nacl content diethylamine of various molarities ph 39 s nacl content imidazole of various molarities ph 39 s nacl content tricine of various molarities ph 39 s nacl content triethanolamine of various molarities ph 39 s nacl content tris of various molarities ph 39 s nacl content elution buffer acetic acid of various molarities ph 39 s nacl content citric acid of various molarities ph 39 s nacl content hepes of various molarities ph 39 s nacl content mes of various molarities ph 39 s nacl content mops of various molarities ph 39 s nacl content pipes of various molarities ph 39 s nacl content lactic acid of various molarities ph 39 s nacl content phosphate of various molarities ph 39 s nacl content tricine of various molarities ph 39 s nacl content 4 add charging buffer once water is at the level of the resin surface 5 add uf water to wash through non bound metal ions once charge buffer washes through illustrative of the various buffering compositions useful in this technique are sample running buffers including but not limited to sodium phosphate buffers at various molarities and ph 39 s charging buffers including but not limited to nickel chloride nickel sulphate copper ii chloride zinc chloride or any suitable metal ion solution elution buffers including but not limited to sodium phosphate buffers at various molarities and ph.