use in injection without ignoring the relevance of other parameters such as the content of impurities it is beyond doubt that for a pharmaceutical compositon for injection the content of ibuprofen after autoclaving is a very important parameter for the present purposes the minimum acceptable content of ibuprofen after autoclaving is considered to be at least 95 of the initial ibuprofen content added to the solution after autoclaving the ibuprofen remaining in the formulations was determined by evaluating the loss produced by the autoclaving determination of ibuprofen was again based on analysis by hplc using the following parameters mobile phase for preparation of the mobile phase 6 g of trifluoroacetic acid was dissolved in 600 ml of water and was adjusted to ph 3 with dilute ammonium hydroxide then 900 ml of acetonitrile was added flow 1 ml min column c18 150 mm u00d7 4 6 mm 5 u03bcm detection 254 nm volume injected 10 u03bcl temperature 25 u00b0 c duration 8 minutes test sample the test sample of ibuprofen is diluted to a concentration between 0 8 and 1 0 mg ml reference sample standard solution of ibuprofen in mobile phase at a concentration between 0 8 and 1 0 mg ml just as in the case of impurities we observe a tendency for a smaller loss of active principle ibuprofen to be obtained on increasing the ph though to a varying extent depending on the container used at ph of 6 5 loss of ibuprofen is.