media is added and left for 10 minutes thereafter complete media and cells are added the phenyl groups on the dextran adsorb to hydrophobic surfaces which will make the surface more hydrophilic the coating procedure is simple and not time consuming mesenchymal stem cells mscs at 40 000 cells well were added to the plates coated according to the invention and the comparison plates after a 21 hour cultivation period cell attachment was assessed no attachment of cells to the tissue culture plastic was observed for the phenyl dextran coated plates all concentrations worked equally well and the costar plates however the nunc plates exhibited an uneven coating with cells attaching to parts of the plates when cells were seeded on phenyl dextran coated plastic they did not attach at all instead they formed free floating spherical cell clusters see fig2 when adding cytodex microcarriers to the costar and phenyl dextran coated plates all cells 20 000 cells well attached to the microcarriers and no cells attached to the bottom of the wells of the microtiter plate the cells grew well on the carriers and no difference in cell morphology was observed when cultured on microcarriers in the phenyl dextran coated plates indicating that the phenyl dextran is not leaking to also coat the carriers or is toxic to the cells the result was that the phenyl dextran coated plates were better than nunc mpc treated and equal to costar ultra low attachment plates when assessing attachment but at a much lower cost the present results indicate that phenyl dextran can completely inhibit cell attachment without altering the proliferation survival or multi potency of cells the coating procedure is a simple fast.