beginning of the tube with no residence time and then for each dwell time the samples are immediately treated with the appropriate quantity of sti and are then frozen after preheating to 80 u00b0 c with a dwell time of 5 minutes the rest of the hydrolyzate which has undergone a dwell time of 20 minutes is pumped into an ultra high temperature sterilizer where it is heated to 125 u00b0 c over a period of 2 minutes to inactivate the enzyme and to sterilize the hydrolyzate after cooling the hydrolyzate is dried by spray drying ______________________________________ ______________________________________ peptides 23 lactose 68 ash 4 fats 2 moisture 3______________________________________ the degree of hydrolysis nitrogen u00d7 100 total nitrogen nt is 18 and the nt 3 56 analysis by sds page confirms the absence of protein bands with 139 u03bcg 5 u03bcg nt and 500 u03bcg 18 u03bcg nt batches of product in particular no bands corresponding to the bsa to the h and l chains of the igg to the ala or to the blg are observed a diffuse coloured band due to the large peptides is observed near the anodic end of the gel id does not show any precipitation line with the anti bsa anti igg anti blg and anti ala sera from a sample of 100 mg dry matter ml before dilution table 3 below shows the effect of the dwell time in the second.