the composition reference is made to article 20 listed previously chronic granulomatous air pouches were raised in mice by known methods the reader is referred to pct ca94 00207 at page 16 for an experimental method in this regard utilizing 0 5 ml freund 39 s complete adjuvant with 0 1 croton oil injected into 1 day old air pouches animals were dosed with saline 0 01 dmso either subcutaneously s c into the nape of the neck or with saline applied topically in 0 1 ml 2 5 hyaluronan vascular casts incorporating carmine in gelatin were formed at termination the tissues weighed dry mg papain digested and the carmine content u03bcg determined by spectrophotometric assay vascularity was calculated as the vascularity index u03bcg mg results are expressed as means u00b1 s e m investigation of the action of tetrahydrocortisol tetrahydrocortisone and tetrahydrocortexolone and the like on inflammation induced angiogenesis in the murine chronic granulomatous air pouch was pursued groups of mice n 8 13 were dosed with tetrahydrocortisol tetrahydrocortisone or tetrahydrocortexolone or the like 0 1 3 0 mg kg in 0 1 ml saline 0 01 dmso s c daily from induction day 0 to termination on day 6 controls were dosed with 0 1 ml saline 0 01 dmso subcutaneously the angiostatic glucocorticoid cortisone requires simultaneous dosing with heparin table 1 to be angiostatic being inactive when administered alone at the sub antiinflammatory dose of 1 mg kg whereas tetrahydrocortisol.