is a stress or aging related gene to prevent plant death during the germination and the recombinant vector psen antiatcad h as an anti sense construct against atcad gene was manufactured sen 1 promoter is specific for a plant gene expressed according to growth stages meanwhile in fig3 the construction of the cloning vector psen see fig3 a and the recombinant vector psen antiatcad h see fig3 b were illustrated as depicted in fig3 bar indicates the bar gene conferring resistance to herbicide barsta phosphinothricin acetyltransferase gene rb right border lb left border p35s camv 35s rna promoter 35s poly a camv 35s rna poly a psen sen 1 promoter nos poly a poly a of nopaline synthase gene the recombinant vector psen atpdz4 was introduced into agrobacterium tumefaciens by the electroporation method agrobacterium transformant was cultivated at 28 u00b0 c until od value reached 1 0 at 600 nm centrifuged at 25 u00b0 c for 10 minutes at 5 000 rpm and then cells were harvested the resulting cells were suspended by using infiltration medium im 1 u00d7 ms salts 1 u00d7 b5 vitamin 5 sucrose 0 005 silwet l 77 lehle seed usa until final od value reached 2 0 at 600 nm 4 weeked arabidopsis thaliana was submerged to agrobacterium tumefaciens suspension in a vacuum chamber left for 10 minutes under 10 4 pa of vacuum and then put in a polyethylene bag for 24 hours after that arabidopsis tumefaciens transformant was cultivated continuously to harvest seed t1 at this moment wild type arabidopsis thaliana and arabidopsis thaliana transformant.