enzyme solution or the crude enzyme powder by a combination of methods properly selected from the group consisting of for example gel filtration methods using sephadex ultrogel bio gel etc adsorption and elution methods using ion exchangers electrophoretic methods using polyacrylamide gels etc adsorption and elution methods using hydroxyapatite sedimentation methods such as sucrose gradient centrifugation and the like affinity chromatographic methods and fractionation methods using a molecular sieve membrane a hollow fiber membrane etc to 25 mm tris hydroxy aminomethane hydrochloric acid buffer was added 1 mm disodium ethylenediaminetetraacetate and 2 mm phenylmethylsulfonyl fluoride followed by adding thereto ammonium sulfate to 10 saturation to 15 ml of the mixed solution ph 7 8 thus obtained was added the posterior portions of 200 insects luciola lateralis purchased from seibu department store co ltd and destroyed by means of physcotron mfd by niti on medical and physical instrument manufacturing company ltd the liquid thus obtained was centrifuged at 12 000 r p m for 20 minutes to obtain 14 5 ml of a supernatant a crude enzyme solution the crude enzyme solution thus obtained was subjected to salting out by the use of ammonium sulfate by a conventional method and the precipitate formed at 30 to 70 saturation as centrifuged.