resultant yellowish clear solution collected the hydrolysate was analyzed for uronic acid using the carbazole calorimetric assay hydrolysate was shown to contain between 0 1 0 3 ha since eggshell membrane samples were diluted approximately 1 10 ha content was between 1 3 ha the insoluble residue was collected and analyzed for collagen glucosamine and chondroitin concentrations the residue was high in hydroxyproline indicating a high concentration of collagen the clear supernatant was stored at refrigerated temperatures the following example relates to the preparation and evaluation of capsules for nutraceutical therapeutic treatments in humans eggshell membrane was separated from eggshell by a mechanical method the separated eggshell membrane was partially dehydrated and powdered using a pulverization mill the powder was sized to produce powder from approximately 100 microns to approximately 500 microns this powder was heat treated and placed in size u201c 0 u201d gelatin capsules containing about 500 mg of eggshell membrane powder an individual experiencing chronic joint pain volunteered to take one capsule per day after approximately 3 days the individual reported a significant reduction in joint pain the pain returned when the individual stopped taking the capsules the following example relates to examination of the preparation using a novel multi analyte immunoassay profile of 60 acute phase reactants cytokines growth factors and hormones using the rules based medicine technology at charles river laboratories eggshell membrane isolates prepared as in example 3 were filtered through 0 2.