present a first discrete signal e g current magnitude would be generated if the second marker is present a second discrete signal magnitude would be generated if both the first and second marker are present a third discrete signal magnitude would be generated and if neither marker is present a fourth discrete signal magnitude would be generated similarly additional probes could also be implemented for increased numbers of multi target detection in certain aspects the sensors and electrodes described herein are integrated into a sensing or analysis chamber for example in a point of care device to analyze a sample from a biological host fig1 depicts an analysis chamber 400 with a pathogen sensor 406 and a host sensor 410 the chamber 400 includes walls 402 and 404 that form a space with which a sample is retained and analyzed at sensors 406 and 410 pathogen sensor 406 includes a conductive trace 408 to connect the sensor 406 to controlling instrumentation such as a potentiostat host sensor 410 is also connected to external or controlling instrumentation with a conductive trace 412 pathogen sensor 406 and host sensor 410 are separated by a distance x in certain aspects the systems methods and devices described herein are integrated into a sensing or analysis chamber for example in a point of care device to analyze a sample from a biological host fig1 depicts an analysis chamber 400 with a pathogen sensor 406 and a host sensor 410 the chamber 400 includes walls 402 and 404 that form a.