the schematic flow diagram of the present invention as shown in step 101 50 u03bcl of ebna1 10 u03bcg ml is added on the modified glass substrate and reacted at 37 u00b0 c for 4 hours the buffer solution 10 mm tris ph 7 3 150 mm nacl is used to wash out un reacted antigens and impurities then according to step 102 the blocking solution 10 mm tris hcl ph 7 6 6 skimmed milk power 0 2 nan3 0 05 tween 20 and 5 mm edta is added after reacting at 37 u00b0 c for 1 hour the glass substrate is rinsed by the buffer solution refer to step 103 in fig1 the diluted serum is added to be tested it is reacted at 37 u00b0 c for 1 hour the specific antibody in the serum is combined with the antigen immobilized on the substrate surface to form the solid phase antigen antibody complex after it is washed by the buffer solution only the specific antibody is left on the substrate and other compositions in the serum will be washed out then 50 u03bcl of biotinylated antibody 0 25 u03bcg ml of concentration is added and reacted at 37 u00b0 c for 1 hour step 104 after washing by the buffer solution again the streptavidin 100 ng ml is added and incubated at the room temperature for 30 minutes as shown in step 105 as shown in step 106 after washing biotinylated dna fragments 1 ng ml is added and reacted at 37 u00b0.