of diabetes which comprises the administration to a subject in need of it of a therapeutically effective dose of a gene therapy composition according to the present invention in a preferred embodiment of the invention the method comprises the administration of the gene therapy composition disclosed herein in a single dose for all the treatment in another preferred embodiment of the invention the method disclosed that the single dose is administered to muscle tissue by means of an unique multi needle injection present invention also disclosed a method of treatment of diabetes which comprises the administration to a subject in need of it of a therapeutically effective dose of a gene therapy composition which comprises at least a vector carrying and allowing the expression of glucokinase gene gck in a preferred embodiment of the method of the present invention the vector is an adeno associated virus based vector in another preferred embodiment of the method disclosed herein the vector comprises the cds having either seq id no 2 or seq id no 4 in another preferred embodiment of the method disclosed herein the vector is selected from aav mhgck or aav gck in another preferred embodiment of the method disclosed herein the gene therapy composition is administered in a single dose for all the treatment in another preferred embodiment of the method disclosed herein the single dose is administered to muscle tissue by.