022 and sm 023 below this laboratory method was used to determine the resistance of house coatings to fungal attack for the method house coatings on standardized paper were used as test substrate and aspergillus niger atcc 6275 and penicillium funiculosum atcc 36839 were used as test fungi the experiments were carried out in petri dishes on dextrose nutrient medium 50 g of the material to be finished were in separate batches mixed with various concentrations of the fungicide to be examined and homogenized for 3 5 min using a basket stirrer paper carrier materials having the dimensions 90 u00d7 270 mm schleicher amp sch u00fcll no 2589 b x 24078 were coated with the test material the paint and or render samples were coated to a wet layer thickness of 250 u03bcm by knife coating the knife had an opening of a width of at least 6 5 cm in the case of renders the layer thickness depended as in practice on the particle size the coated carrier materials hereinbelow referred to as test bodies were subsequently dried in a horizontal position for five days the test bodies were watered in running tap water of 15 u00b1 5 u00b0 c and a flow rate of 1 l min for 72 hours and subsequently dried for two days the cross section of the container for the watering in the direction of flow was 1000 u00b1 500 cm 2 from the pretreated test bodies sample bodies having a diameter of 5 cm were punched out and sterilized in a co 60 source using at least 10 kgy the sabouraud dextrose agar which had solidified in the petri dish was inoculated with 0 2 ml of spore suspension 10 7 spores ml and plated out using a sterile drigalski spatula or.