still inhibition on tnf u03b1 release induced by lps and cpg dna when kb was added more than 120 min after stimulation it would no longer have inhibitory activity on tnf u03b1 release in addition under serum free conditions kb can still inhibit the release of tnf u03b1 in raw264 7 cells induced by lps and cpg dna in a dose dependant manner which eliminated the possibility that kb play an indirect role in inhibition on lps and cpg dna by acting on the serum protein the results were shown in fig2 wherein fig2 a shows the inhibition of kb on tnf u03b1 release in raw264 7 cells induced by lps and cpg dna in which kb was loaded after preincubation with lps or cpg dna fig2 b shows the inhibition of kb on tnf u03b1 release in raw264 7 cells induced by lps and cpg dna in which kb was loaded at various time points fig2 c shows the inhibition of kb on tnf u03b1 release in raw264 7 cells induced by lps and cpg dna in which kb was loaded without serum detection of inhibitory activity of kb on release of tnf u03b1 and il 6 in raw264 7 cells induced by various pathogen associated molecular patterns raw264 7 cells were stimulated by six pathogen associated molecular patterns lps 100 ng ml cpg dna cpg 10 u03bcg ml pam3csk4 pam3 10 u03bcg ml poly i c i c 20 u03bcg ml tnf u03b1 50 ng ml and il 1 u03b2 50 ng ml and added with kb final concentration of 200 u03bcm in the meantime according to the method in embodiment 6 the supernatant was collected the concentration of tnf u03b1 was detected according to the manufacturer 39 s instructions of elisa kit result.