or from prosthesis bone interface located between the prosthesis and the bone solid tissue samples were cut into small pieces in a sterile petri dish plate and were placed in eppendorf tubes water for injection was added to eppendorf tubes containing tissue samples and the tubes were whirled for 2 minutes the water after whirling was utilized for testing the peptidoglycan or beta glucan measurement patient 8 fig1 c in each series of testing of clinical samples a negative control experiment was included in the same microplate under the same test conditions water for injection used for dilution of synovial fluid samples or for obtaining periprosthetic tissue broth was used as negative control influence of freezing of infected synovial fluid samples on the result of ppo test four samples of infected synovial fluid from patients 1 2 4 and 5 were assessed by slp test before and after 7 10 days of storage at u2212 20 u00b0 c graphs are presented in fig1 a 14 b 14 c and 14 d measurement of biomarkers of bone destruction in the blood of patients with periprosthetic joint infection out of 9 blood samples of patients with periprosthetic joint infection that were tested for different biomarkers in five samples the levels of osteopontin were found to be higher.