25 24 225 3 07 12 5 32 5 4 5900 1 68 12 5 32 5 24 57 3 69 12 5 32 5 24 34 3 910 12 5 32 5 24 94 3 411 12 5 32 5 24 44 3 712 12 5 32 5 44 33 3 913 12 5 40 24 27 4 014 20 25 4 31400 0 915 20 25 44 16 4 216 20 32 5 24 24 4 017 20 40 4 5 4 718 20 40 44 0 5 4______________________________________ the results demonstrate that the bactericidal activity of edc is dependent on the concentration of edc the temperature of incubation and the duration of incubation in additions it can be seen that edc at 20 mm concentration kills all the spores on cross linked tissue between 4 and 44 hours at 40 u00b0 c a logarithmetic reduction of about 4 7 was obtained after 4 hours of incubation at 40 u00b0 c and no survivors were present after 44 hours of incubation in additions a logarithmetic reduction of about 4 was achieved at 40 u00b0 c after 44 hours of incubation in the presence of only 5 mm edc indicating that edc is a potent bactericidal agent an experiment was designed to test the bactericidal activity of the edc treatment using two sequential inoculations of at least.