agar 17 5 compost extract 400 tap water 600 3 glycerol 20 0 glycine 2 nacl 1 k sub 2 hpo sub 4 1 feso sub 4 7 h sub 2 o 0 mgso sub 4 7 h sub 2 o 0 caco sub 3 0 distilled water to 1 liter ph adjusted to 7 0______________________________________ serratia sp sc 11 482 a t c c no 39006 were isolated from salt marsh water samples containing the bacterium by filtering 50 ml of the sample through a millipore filter washing the cells from the filter pad and spread plating onto a medium containing ______________________________________ grams______________________________________l arginine 1 0glycerol 12 5k sub 2 hpo sub 4 1 0nacl 1 0mgso sub 4 7h sub 2 o 0 5feso sub 4 7h sub 2 o 0 01cuso sub 4 5h sub 2 o 0 001znso sub 4 7h sub 2 o 0 001mnso sub 4 7h sub 2 o 0 001distilled water to 1 literph adjusted to 7 0______________________________________ after 3 days incubation at room temperature colonies of serratia sp sc 11 482 a t c c no 39006 were isolated from the plated samples the isolated colonies were then grown on a medium containing ______________________________________ grams______________________________________yeast extract 1 0beef extract 1 0nz amine a 2 0glucose 10 0agar 15 0distilled water to 1 literph adjusted to 7 3______________________________________ the antibiotic 1 carba 2 penem 3 carboxylic acid is produced by cultivating serratia sp sc 11 482 a t c c no 39006 at or near room temperature about 25 u00b0 c under submerged aerobic conditions in an aqueous nutrient medium containing an assimilable carbohydrate and nitrogen source.