the enzyme displayed activity in a range of ph 6 to ph 11 with a small activity plateau at ph 8 to ph 9 in order to determine the ph stability the enzyme was incubated for 1 h at 25 u00b0 c at varying ph values ranging from ph 2 to ph 12 after this the enzyme solutions in concentrations of 0 1 u03bcg ml were incubated with 0 1 mg ml bsa and 5 mg kg ergotamine in teorell stenhagen universal buffer ph 7 0 at 25 u00b0 c also in this case an activity plateau appeared this time in the range of ph 6 to ph 9 with a strongly decreasing activity outside this range the activity in this range ensures the technological application of the enzyme with the sequence id no 1 as a feed additive expression of the enzyme with the sequence id no 1 in picha pastoris the gene with the sequence id no 2 was cloned into pgapz alpha c transformed into p pastoris and expressed using standard methods the expression vector pgapz alphac with the gene having the sequence id no 2 is illustrated in fig3 a degradation assay was carried out in 50 mm sodium phosphate buffer ph 7 0 with 5 mg kg ergotamine at.