3 6 deoxy u03b1 l mannopyranosyl oxy 5 14 dihydroxycard 20 22 enolide 1 u03b2 3 u03b2 5 u03b2 11 u03b1 and digoxin solid phases were prepared in a similar manner ouabain triacetate ouabain and digoxin were attached to bovine serum albumin following the general scheme described in biochemistry 9 331 1970 the albumin conjugates were subsequently coupled to trisacryl u00ae previously activated with carbonyl diimidazole in a manner similar to that described by bethell j biol chem 254 2572 1979 the antigencity of cardiac glycoside analogs was first determined using the tdx analyzer and the abbott digoxin ii assay commercially available from abbott laboratories abbott park il 60064 analogs were evaluated as aqueous solutions in tdx dilution buffer several analogs were also subsequently evaluated using the new digoxin assay described herein see generally example iv using a ouabain triacetate solid phase prepared in accordance with example iib analogs evaluated were str3 wherein r 1 u2550 r 2 u2550 r 3 u2550 ac x u2550 o y 1 z 1 and y 2 z 2 u2550 a bond connecting the two carbon atoms to which they are attached a r 1 u2550 r 2 u2550 r 3 u2550 ac x u2550 o y 1 u2550 oh.