u00b0 c for the test 100 u03bcl supernate was taken to conduct elisa examination and commercial available elisa kit was used to test the amount of inflammatory cytokines the results were shown in fig2 and the detailed data were shown in table 3 it can be seen from fig2 compared with the sham operation group the content of il 2 fig2 a il 4 fig2 c il 17 fig2 d increased remarkably in lungs of copd mice it indicated that 6 mg kg rupatadine treated group could reduce the content of various inflammatory cytokines in lungs of copd mice while rupatadine did not affect the content of ifn u03b3 fig2 b which plays an important role in tissue repair so it was proved that rupatadine can adjust body immune reactions directionality he staining method is also named hematoxylin eosin staining method hematoxylin dye liquor is alkaline and mainly colors intranuclear chromatin and intracytoplasmic ribosome into bluish violet eosin is acidic dye and mainly colors the components in cytoplasm and extracellular matrix the lung tissues on the right lower lobe was cut fixed by 4 wt paraformaldehyde and then embedded by paraffin the largest cross section of the paraffin embedding lung tissue was stained by he staining method and the result was shown in fig3 fig3 showed that the area of alveoli increased remarkably in the lungs of copd mice fig3 b.