expression levels of the biomarker and the predetermined standard in the suspected cancer region application_number US 201113099694 A description the present invention has been described in an illustrative manner and it is to be understood that the terminology used is intended to be in the nature of description rather than of limitation many modifications and variations of the present invention are possible in light of the above teachings therefore it is to be understood that within the scope of the appended claims the invention may be practiced otherwise than as specifically described identification of endogenous tiam2s expressions in normal tissues and tumor tissues with semi quantitative rt pcr and western blot the cdna panels for different human tissues including normal brain tissue brain tumor tissue normal liver tissue liver tumor tissue normal thymus tissue thymus tumor tissue normal breast tissue breast tumor tissue normal prostate tissue prostate tumor tissue normal pancreas tissue pancreas tumor tissue normal colon tissue and colon tumor tissue were purchased from clontech palo alto calif and biochain hayward calif tiam2s specific pcr primers were used to amplify tiam2s the pcr parameters were 95 u00b0 c for 5 minutes followed by 30 cycles of amplification at 95 u00b0 c for 30 seconds 64 u00b0 c for 20 seconds and 72 u00b0 c for 20 seconds with a final extension at 72 u00b0 c for 10 minutes the pcr.