voltage and amplitude gains on the fsc and ssc detectors the detectors for the three fluorescence channels fl1 fl2 and fl3 were adjusted by running cells stained with purified isotype control antibody followed by alexa fluor 488 conjugated secondary antibody such that cells had a uniform peak with a median fluorescent intensity of approximately 1 u2013 5 units live cells were acquired by gating for fsc and propidium iodide exclusion for each sample approximately 10 000 live cells were acquired for analysis and the resulted presented in table 3 table 3 tabulated the mean fluorescence intensity fold increase above isotype control and is presented qualitatively as less than 5 u2212 5 to 50 50 to 100 above 100 and in parenthesis the percentage of cells stained representative histograms of 7bd 33 11a antibodies were compiled for fig1 1a245 6 antibodies were compiled for fig2 11bd 2e11 2 were compiled for fig3 and evidence the binding characteristics inclusive of illustrated bimodal peaks in some cases 11bd 2e11 2 displayed specific tumor binding to the breast tumor cell line mda mb 231 both 7bd 33 11a and 1a245 6 displayed similar binding to cancer lines of breast mda mb 231 and mcf 7 colon lung ovary and prostate origin and differential binding to one of the breast cancer cell lines mda mb 468 there was binding of all three antibodies to non cancer cells however that binding did not produce cytotoxicity this was further evidence that binding was not necessarily predictive.