1 7 the 12 cannula pipette with disposable pipette tips is moved to trough 1 to aspirate the diluted microsome volume to mix the m c b the station then rocks to mix the contents of the trough the 12 cannula pipette with disposable pipette tips is moved to trough 1 to aspirate the diluted microsome volume and then is moved to the transfer nest to dispense the diluted microsome volumes to the reaction plate 96 wells 8 rows with 12 wells row in the first four rows thereof 8 step 7 is repeated to fill rows e h of the reaction plate 1 9 the filled reaction plate 1 plate is then transported to a heater site for a u201c pre incubation time u201d sufficient to raise the temperature of the contained microsome solution to a pre determined temperature and reaction plate 1 u201c pre incubation start time u201d is recorded u201d the 12 pipette and single pipette cannulas are cleaned for re use during the test run they are replaced for different test runs the pre incubation time is adjusted to be the same for each reaction plate in the test run cryovial 2 is moved at about this time from frozen to thaw position actual time of such movement is determined based on equivalent time for each of the reaction plate runs 10 a pre prepared 96 well s i plate 1 is picked up and transferred to the robbins hydra 96 pipette system using a second gripper.