cell of human sw620 atcc or hct116 atcc was transferred to t25 flask culture 7 5 ml by rpmi1640 gibco br medium or dmem medium gibco br containing 10 fbs fetal bovine serum respectively thereafter the cell was incubated for 12 hours at 37 u00b0 c in incubator after the cell was incubated for 12 hours 7 5 u03bcl of dmso was added to a culture used as control group for the final concentration to be 0 1 in order to use in experiment samples of various concentrations were prepared by dissolving the cinnamaldehyde derivatives of the present invention in dmso 7 5 u03bcl of the prepared samples was added to a culture used as experimental group cell of control or experimental group was incubated for 48 hours for cell cycle analysis incubated cell was removed from medium thereafter the incubated cell was separated from the flask by trypsin thus the cell was centrifuged at 300 g for 5 min the cell was washed with phosphate buffer solution pbs to remove the residue medium from the cell 3 ml of ethanol 70 was added to the cell the cell was fixed by being positioned at u2212 20 u00b0 c for 12 hours the fixed cell was centrifuged at 300 g for 3 min and washed with the cold pbs for two times to remove residual ethanol the cell solution prepared by adding 500 u03bcl of pbs to the cell was well mixed to produce the homogeneous state 100.