most preferably in distilled or deionized water as used in the present application 34 microbial contamination 34 refers to the growth of any unwanted micro organisms e g bacteria or fungi in a cell or tissue culture medium as used in the present application 34 cell or tissue culture medium 34 34 culture medium 34 and 34 medium 34 refer to a solid substrate including gels having nutrients and other materials used for the growth of micro organisms and plant and animal tissues in culture including but not limited to such substrates in which a plant seed will germinate a plant can be maintained or grown an isolated plant or animal organ or animal tissue can be maintained propagated or differentiated or one or more isolated plant cells plant cell aggregates or plant cell protoplasts may be maintained propagated or differentiated and which is to be maintained in a sterile condition i e substantially free of microbial contamination the terms 34 cell or tissue culture medium 34 34 culture medium 34 and 34 medium 34 are further intended to refer to solid media containing water and an appropriate mixture of mineral salts the culture medium may further incorporate in appropriate concentrations phytohormones including for example auxins cytokinins or gibberellins vitamins such as one or more b vitamins one or more carbon sources including for example sucrose or glucose and one or more undefined growth enhancers such as coconut milk as generally known in the art for example the mineral salts may be selected from any number of commercially available mixtures e g from sigma chemical.