tt and a whole protein extract of candida albicans c albicans the results from these examples show that culturing human t and b cells together in vitro at a u02dc 1 1 ratio versus the ratio of t and b cells naturally found in the blood gave stronger antigen responses by both analysis of activation and proliferation flow cytometry and antibody production elispot although the preferred ratio of t cells b cells is u02dc 1 1 the ratio of t cells b cells can range from u02dc 1 10 to u02dc 10 1 in the cultures of the examples u201c t cells u201d included both cd4 and cd8 t cells in peripheral blood the t total t cells b cell ratio is u02dc 7 1 in the lymph node the t total t cells b cell ratio is u02dc 1 1 6 in the germinal center the t cell b cell ratio is u02dc 1 8 and there the t cells are primarily cd4 t cells in the results of the experiments shown engineered serum free media x vivo was used though we have also used serum e g human bovine in other experiments data not shown dendritic cells dcs were generated from.