cm including a porous silica support having a 5 u03bcm particle size and a pore size of 100 nm was used although many other types of cation exchange columns can be employed two mobile phases were used in the hplc unit mobile phase i was composed of 10 mm bis tris and 1 mm kcn and had a ph of 7 0 mobile phase 2 was composed of 10 mm bis tris 1 mm kcn and 200 mm nacl and had a ph of 6 6 the amounts of mobile phase 1 and mobile phase 2 in a mixture thereof are represented below as volume percentages relative to the total volume of the mixture the test samples contained whole blood hemolysate prepared by dilution of one part whole blood with from about 20 to 500 parts purified water preferably from about 50 to 200 parts purified water and ideally 100 parts purified water sodium azide 0 001 was added as a preservative the injection volume was from about 1 u03bcl to 25 u03bcl preferably from about 2 5 82 l to about 10 u03bcl and ideally 5 u03bcl of the diluted i e hemolyzed sample when the startup method was selected the.