fig1 virus stocks were titrated on the various glioma cells and control bgmk or rk13 cells virus titres derived from the gliomas were compared to the control levels and a value of viral replication efficiency was estimated based on these results none of the gliomas supported viral growth to the levels observed in the control lines however some viruses vmyx135ko vmyx63ko and vmyx136ko were more efficient than other knockouts as well some glioma lines supported more replication u87 u343 and u373 fig2 various human gliomas were infected with a range of vmyx hrkos twenty hpi the infected supernatants were collected and concentrated 10 u00d7 fifteen microlitres of concentrated sups were separated on a 12 sds page gel and probed with anti serp1 mab late mv gene product the blots were stripped and probed for early gene expression with anti m t7 pab early mv gene product the results suggest that several vmyx hrkos are restricted in their transit from early to late gene expression including vmyxt2ko vmyxt4ko and vmyxt5ko and in two glioma lines u87 and u37 vmyxt4ko does not even undergo early gene expression fig3 cells were seeded in 48 well dishes and infected cells were collected at the times indicated virus was released from the collected cell pellets and titrated back onto bgmk cells.