5 which encodes a 117 amino acid product the fourth intron of this sequence includes two regions which show strong homology with a region found only in gage 1 there is a 561 base pair segment positioned in between these regions at nucleotides 7109 7659 which corresponds to a truncated l1 retroposon which belongs to the family of long interspersed repeated elements or u201c line u201d as described by hutchinson et al in berg et al eds u201c mobile dna u201d am soc microbiol 1989 incorporated by reference the line element is flanked by a perfect 13 base pair target site duplication and contains part of the reverse transcriptase coding region the 3 u2032 untranslated region and the poly a tail of the original retroposon a cosmid library was prepared using genomic dna from renal cell carcinoma cell line le9211 rcc following the methodologies described by lurquin et al cell 58 293 303 1989 and screened using the southern hybridization method set forth in example 2 using the same probe a cosmid was identified which contained genomic dna for gage 8 its structure was the same as that of gage 7b including the line insertion discussed supra these experiments describe how the chromosomal location of the gage genes was determined southern blot analysis was carried out on a panel.