microbe was obtained from the inner bark of a specific mappia species belonging to north western himalayan agro climatic region but may not restrict to this species alone the aerial tissue fragments from a fully grown tree or the seeds thereof of the genus nothapodytes foetida a medicinal plant which grows widely throughout india including in the north western himalayan agro climatic region as well as from southern konkan forests the medicinal plant 39 s best suited habitat of north western agro climatic region is commonly found in north western ghats of india this tissue fragments were placed on agar medium for 2 7 days and isolating the culture u2014 an endophyte from inner stem bark fragments after surface sterilisation of bark fragment with 50 70 ethanol for 1 20 minutes a fungal culture designated as mttc 5124 was isolated from inner bark of mappia tree and grown on peptone dextrose broth pda peptone 10 dextrose 40 gl u2212 1 ph 5 6 and incubated at 28 u00b1 2 u00b0 c in an incubator shaker 200 220 rpm for 6 7 days the fungus grows into a mass of mycelium and sporulates sparsely during incubation the mycelial mat along with the spores was filtered through the whatman filter paper under vacuum till all the broth was removed the pellet was washed with tris hcl ph 6 7 buffer and resuspended in the same buffer in the ratio of 1 4 the mycelial mat was homogenized in a homogenizer and centrifuged at 4 u00b0 c 10 000 12 000 rpm for 15 30 min the supernatant was extracted with chloroform and methanol 4 1 mixture to further confirm the presence of cpt on hplc and was found identical with authentic control the left over residue from centrifuged sample was.