maximum rotor js 4 2 beckman u2122 j6 b the appendix of the bag containing the red blood cells residue was closed using the clamp which is usually used for retaining leukocytes the bag was turned upside down suspended and the supernatant was transferred in a six line clx bag miles canada using the proximal line the order of the lines used is always from the proximal to the distal centrifuged for 8 min at 5000 g brake maximum rotor js 4 2 beckman u2122 j6 b the bag was placed on a plasma press miles canada and the maximum of supernatant was removed in a beaker the line was closed using an aluminum ring the bag containing the centrifuged platelet concentrate was placed on a weigh bridge sartorius u2122 lc 4800 p and was injected with 48 g of plasma lyte a u2122 using one line of the clx bag the bag opening was temporarily blocked using a hemostatic claw or an aluminum ring to prevent a relux in the lines the platelet concentrate was left to rest for one hour and was then agitated for about one hour or until complete resuspension on a horizontal shaker new brunswick scientific model g20 at 25 rpm and 22 u00b0 c the bag was placed on a weigh bridge sartorius u2122 lc 4800 p and was injected with 360 g of plasma lyte a u2122 using one line of the clx bag mixed well the content of the bag was transferred to a graduated cylinder for the cellular culture the volume was measured 1 ml is taken to at 5 ml polypropylene tube in a.