50 mg kg bw day was given in combined form to all the 10 rats along with sleep deprivation stress on 7 th day after two hours of administration of test drug different parts of the rat 39 s brain were dissected and 5 ht melatonin gaba glutamate and histamine were measured group iv the modern drug u201c ibrium u201d was given in the dose of 2 mg kg day for 7 days along with sleep deprivation stress on 7 th days the animals were sacrifice and brain biogenic amines were estimated in different parts of rat 39 s brain the value obtained were compared with each other group normal vs sleep deprivation stress and sleep deprivation stress vs sleep deprivation stress test formulation and sleep deprivation stress vs modern drug treatment group apart from initial at 7 day before sacrificing the animals circulating biogenic amines were also measured in all the three groups as per the object of the present experimental study the effect of test formulation containing the hydro alcoholic extract of three plants nyctanthes arbortristis ocimum tenuiflorum and hippophae salcifolia in effective doses was orally induced to animals following sleep deprivation stress the beneficial role is evaluated on various brain biogenic amines it is observed that 7 days sleep deprivation stress exerted marked alteration on brain biogenic amines i e 5 ht melatonin gaba glutamate and also a biogenic amines like gaba glutamate 5 ht.