i dna capture column is evaluated by the following assay mix 50 ng of cy3 labeled recovered dna with 50 of cy5 labeled original sheared genomic dna and hybridize to a bac clone microarrays which contain clones of variable amount of repeats in discrete spots in a proper microarray buffer for 3 hours at 37 u00b0 c after the hybridization use distilled water to rinse of all residual hybridization solution and dry the array by blowing compressed air to the array image the array in both cy3 and cy5 channel the effect of repeat removal is shown by significant loss of signal in the cy3 channel this effect is confirmed by the following assay mix 2 u03bcg cy3 labeled recovered genomic dna and 2 u03bcg cy5 labeled original sheared genomic dna and 50 u03bcg cot i for repeat suppression the probe mix is hybridized to a bac clone array in an array hybridization buffer at 37 u00b0 c for 16 hours rinse off the hybridization solution and image the array after drying the effect of repeat removal is indicated by the significantly higher signal level in the cy3 channel compared to that of cy5 channel due to the fact that in equal amount of labeled probe the recovered genomic dna contains more unique sequence than the original genomic dna the above assay results were further confirmed by cy3 cy5 dye swap experiments normalizing the copy number of highly expressed genes in lung tumor tissue using microfiber filter column highly expressed genes in lung cancer samples were identified from expression data in the public database genes with an expression level 10 times above the median levels of all expressed genes in.