entire implant surface the chloroform quickly evaporates at room temperature leaving the excipients in the form of a thin film the second coating methodology involves the production of a phospholipid suspension in an aqueous media the phospholipid was weighed out and dissolved in 100 u03bcl hplc grade chloroform at a concentration of 222 u03bcmol ml a thin film of phospholipid was produced on the wall of a glass round bottomed flask as described in example 1 1 ml of de ionised water was then added stepwise to the flask and the flask shaken resulting in a phospholipid suspension being produced 40 u03bcl of the phospholipid suspension was then gently layered onto a defined area of the material surface the suspension was then dried onto the surface of the implant material by means such as air drying freeze drying or rotary evaporation this results in a thin film of phospholipid being deposited onto the surface of the material the following spot sizes were produced on the ti pore300 implant coupons in the case of 444 u03bcmol ml solution the spot concentration was estimated at 5 u03bcmol cm 2 spot size produced spot size produced for for total amount phosphatidylserine phosphatidylinositol excipients u03bcm ml formulation mm formulation mm 444 7 3 222 9 6 5 111 10 6 56 11 7 induction of calcium phosphate precipitation on the surface of a metal surgical implant using a phospholipid formulation containing phosphatidylserine this solution was then used to undertake a serial dilution to produce standards at 444 222 111 and 56 u03bcmol ml chloroform 5 u03bcl of each of the 4 standards were then applied onto distinct areas of a clean titanium implant coupon u2014 ti pore300 u2014 previously sonicated 3 times in hplc grade chloroform for 30.