negative control does not generate a significant signal comparative analysis of the results of the 34 mtd 2 34 test the tma amplification part of which was carried out manually or automatically according to the invention therefore demonstrates that it is possible to change over from a manual test to a device according to the invention and that the single use card according to the invention makes it possible to carry out all the biological steps of a test which as in manual mode and equally sensitively detects the equivalent of 100 bacteria the benefit of such a device according to the invention is great in particular in the field of molecular biology the techniques of which such as tma make it possible to detect pathogens sensitively and quickly nevertheless in view of their performance these techniques are very sensitive to contamination from the environment or introduced during handling for carrying out intermediate steps of adding or mixing reagents therefore leading to erroneously positive tests the present invention makes it possible to carry out the amplification steps under confinement and isolation and to sequence them if appropriate with the detection steps starting from the introduction of a sample into the single use device the latter can therefore contain ready to use reagents in stabilized form which can be packaged and prepared in a contamination free controlled environment an experimenter therefore needs merely to ensure the absence of contamination during the steps of preparing the sample before the test is carried out and when this is being introduced into the card more generally when the detection steps are also incorporated into the card disposal of and destruction of the.