acetate then an hplc analysis was performed the total recovery rate of sesamin and sesamolin was calculated and 97 9 was obtained results were shown below in table 1 the methods performed in examples 2 to 5 were the same as that performed in example 1 however acetone methanol 95 ethanol and n hexane ethyl acetate 1 1 were used as eluting solvents instead of ethyl acetate the total recovery rates of the obtained sesamin and sesamolin were calculated they were 95 8 95 8 89 6 and 95 8 in that order results were shown in table 1 the method performed in example 6 was the same as that performed in example 1 except that petroleum ether was used to replace n hexane the result showed that the total recovery rate of sesamin and sesamolin was 96 4 one hundred grams of silica gel 70 to 230 mesh was balanced using n hexane and were packed in a column 4 4 cm u00d7 14 cm in 500 ml of n hexane 500 g of sesame oil was added and thoroughly mixed to obtain a mixture the mixture was passed through the column and was eluted with n hexane until the eluate turned colorless then the eluate was eluted by ethyl acetate until the eluate again turned colorless the solvent was removed from the eluate by evaporation under a reduced pressure to obtain oil then the oil was set overnight to precipitate the formed crystals the precipitant was collected by filtration oil was removed from the precipitant by washing with n hexane to obtain 1 7 g of crude crystals an hplc analysis was performed results showed that the sesamin content was 77 5 the sesamolin content was 16 8 and the total content was 94.