2x concentrated o 39 farrell buffer as per o 39 farrell et al j biol chem 250 4007 1974 the disclosure of which is incorporated by reference and boiled for 5 minutes the boiled material was then subjected to electrophoresis in 6 to 15 acrylamide gradient gels fig6 shows the results of the analysis on these gels i e sds page analysis referring to the lanes lanes c and d involved tests where excess 34 cold 34 ril 9 was used and all others where it was not lanes a and b are the product of incubation without dss whereas the others did use this lanes b d and f represent material where the n glycosidase f digestion was used thus lanes e and f are most probative of the il 9r molecule because these lanes represent situations where radioactive il 9 was used followed by dss cross linking lane f represents an experiment where the enzyme was used so comparison of the sds page gels for e and f gives evidence of the effect of glycosylation on the molecule the analysis showed two radioactive bands of 22 and 86 kilodaltons these correspond to the free and complexed ril 9 molecule respectively when the cross linking agent was not used or excess 34 cold 34 ril 9 was used the complex disappeared this suggests that the 86 kd component.