pp 169 170 some examples of salt concentrations and temperature combinations are as follows in order of increasing stringency 2 u00d7 sspe or ssc at room temperature 1 u00d7 sspe or ssc at 42 u00b0 c 0 1 u00d7 sspe or ssc at 42 u00b0 c 0 1 u00d7 sspe or ssc at 65 u00b0 c detection of the probe provides a means for determining in a known manner whether hybridization has occurred such a probe analysis provides a rapid method for identifying toxin encoding genes of the subject invention the nucleotide segments which are used as probes according to the invention can be synthesized using a dna synthesizer and standard procedures these nucleotide sequences can also be used as pcr primers to amplify genes of the subject invention variant toxins certain toxins of the subject invention have been specifically exemplified herein since these toxins are merely exemplary of the toxins of the subject invention it should be readily apparent that the subject invention comprises variant or equivalent toxins and nucleotide sequences coding for equivalent toxins having the same or similar pesticidal activity of the exemplified toxin equivalent toxins will have amino acid homology with an exemplified toxin this amino acid identity will typically be greater than 75 or preferably greater than 85 preferably greater than 90 preferably greater than 95 preferably greater than 96 preferably greater than 97 preferably greater than 98 or preferably greater than 99 in some embodiments the amino acid identity will typically be highest in critical regions of the toxin which account for biological activity or are involved in the determination of three dimensional configuration which ultimately is responsible for the biological activity in this regard certain amino acid substitutions are acceptable and can be expected if these.