binary vector pga482 an et al 1985 embo j 4 277 this construction places this mutant phaseolin gene within the right and left borders of the binary plasmid now referred to as p u03bc3 3c 1 and along side of the plant expressible npt ii gene which is used for selection and identification of transformed plants the structure of binary plasmid pu3 3c 1 is shown in chart 1 this binary plasmid has to be transferred into various agrobacterium strains i e a208 c58 c58 707 lba4404 and a4rs etc the method described here can be used to transfer the binary plasmid p u03bc3 3c 1 intovarious plant species e g common bean soybean and other large seeded plants in addition multiple copies of the phaseolin minigene can be placed into the binary plasmid by subcloning the ncoi to bamhi fragment 3kb fragment from ppv3 3 cdna into ncoind bamhi digested clone ppr 8 8 g available from j slightom the upjohn company kalamazoo mich which replaces the genomic part with the cdna region of ppv3 3 cdna this cloning experiment results in obtaining subclone ppv8 3 cdna which contains an upstream bglii site slightom et al 1983 proc natl acad sci 80 1897 which allows for the isolation of a bglii bamhi 3 3 5 kb fragment which was recloned into the bamhi digested plasmid ppv3 3 cdna the orientation of the new phaseolin insert s can be.