from water there was obtained off white crystals melting at 191 u00b0 3 u00b0 c a reaction mixture of 204 g of badd 2 3 dihydroxanthotoxol baddend iadd 4 39 5 39 dihydroxanthotoxol iaddend of part d 136 g of dimethyl sulphate 828 g of potassium carbonate and 9 liters of acetone was refluxed with stirring for 16 hours the reaction mixture was then cooled and filtered and the filter cake washed with acetone the acetone solution was concentrated to approximately 2 liters and poured into 4 liters of 1 percent sodium hydroxide solution with good stirring the product was filtered and washed with water until the ph was neutral it was then washed with a little cold acetone and finally air dried there was obtained 185 g 85 percent yield of crude badd 2 3 dihydroxanthotoxin baddend iadd 4 39 5 39 dihydroxanthotoxin iaddend melting at 158 u00b0 160 u00b0 c this crude product was used directly in step f on successive replications the yield varied from 80 to 85 percent on recrystallization from benzene there was obtained a white solid melting at 159 u00b0 160 u00b0 c a reaction mixture of 218 g of badd 2 3 dihydroxanthotoxin baddend iadd 4 39 5 39 dihydroxanthotoxin iaddend of part e 281 g of 2 3 dichloro 5 6 dicyano 1 4 benzoquinone and 3 liters.