larger than the average surface area of the cells see black arrowhead in fig4 c conversely p19 cells on hcam showed similar morphology to the cells seeded in ccm on tops and similar doubling values doubling value 2 5 on tops versus 2 6 on hcam specifically fig4 a 4d are directed to proliferation of p19 cells seeded on pems and hcam after resuspension in sucrose fig4 a shows the change in the number of cells seeded in sucrose media after 24 hours doubling value values are approximately 2 for cells on the hcam surface whereas cells on pems show values of less than 2 when cells were suspended in sucrose averages of 89 cells frame 44 cells frame and 25 cells frame were observed for tops pems and hcam respectively error bars represent one standard deviation fig4 b shows representative phase contrast images of p19 cells on tops pems fig4 c and on hcam fig4 d 24 hours after seeding cells on pems and hcam were suspended in sucrose for 15 min black arrowhead in fig4 c indicates cell with larger than average surface area and white arrowheads indicate weak cell attachment to the surface of the pems the scale bar in fig4 c is 100 u03bcm the viability of p19 cells was assessed using a live dead viability assay from invitrogen corp the viability results in fig5 show that 99 or more of the cells were viable 48 hours after cell seeding on hcam and 96 of the cells were viable on the pems also the results indicate that p19 cells can be exposed to sucrose for at least 60 minutes with no significant change in viability specifically fig5 illustrates percent viability of p19 cells on the hcam cells.