isolate and is described in u s pat no 5 439 678 the lactobacillus acidophilus used herein is a gram positive rod well known in the dairy industry each organism was grown separately in appropriate media and under conditions which favored viability the fermentation patterns for each organism are known in the art and have been described previously for lactobacillus for example see silva m et al antimicrobial substance from a human lactobacillus stain antimicrobe agents chemother 31 1231 1233 1987 for cultivating strains of l reuteri u s pat no 5 439 678 should be reviewed all of the data and teachings of u s pat no 5 439 678 are incorporated herein by reference after fermentation and isolation using techniques known in the industry the organisms were combined along with a carrier cryoprotectant whey protein concentrate and freeze dried other carriers cryoprotectants such as lactose or maltodextrin can be used the cultures were combined using dry blending techniques and the resulting inventive culture mixture had the following concentrations this three part culture was manufactured and supplied by biogala biologics inc raleigh n c and is commercially available from biogaia biologics the flavor packets for the clinical study including.