ml 100 ml of lipoaspirate will be saved for later treatment and reformulation during the preparation phase of the protocol prior to liposuction the abdominal wall is preferably irrigated with a sterile saline solution and dilute epinephrine the surgeon may utilize a tumescent solution that is a mixture of 1 lidocaine and 1 mg 1000 ml epinephrine in normal saline solution however this liposuction process can be performed without the tumescent solution in some patients this process is a current standard of care to facilitate the aspiration process and to reduce the bleeding and pain after the procedure this procedure can be performed under general or sedation and local anesthesia decisions regarding utilization of specific anesthesia techniques will be made by the medical professional undertaking the procedure following aspiration a portion of the adipose tissue from the patient may be treated to isolate a fraction enriched in adipose derived stem cells in certain embodiments the enrichment protocol includes three major subprocesses preparation incubation and washing yoshimura et al proved the efficacy of this process while extracting serovascular fraction svf of adipose tissue and utilizing it to treat facial lipoatrophy yoshimura et al u201c cell assisted lipotransfer for facial lipoatrophy efficacy for clinical use of adipose derived stem cells u201d dermatol surg 34 9 1178 85 2008 which is hereby incorporated by reference a portion of the lipoaspirate will be.