in pancreatic cholesterol esterase 34 j biol chem 266 4033 4036 1991 the feasibility of using a active site inhibitor to reduce cholesterol absorption has been reported by bailey et al in 34 inhibition of dietary cholesterol ester absorption by 3 bcp a suicide inhibitor of cholesterol esterase 34 biochem soc trans 23 408s 1995 intragastric administration of a single dose of 3 benzyl 6 chloropyrone to rats simultaneous with feeding of cholesterol ester resulted in a 60 drop in cholesterol absorption which resulted from a 63 inactivation of lumenal ce activity in vitro ce was inactivated by this pyrone with a half life of 100 seconds 3 benzyl 6 chloropyrone is a prototype haloenol lactone that has been developed as a suicide inhibitor of chymotrypsin although it is not highly selective as described in daniels et al in 34 haloenol lactones potent enzyme activated irreversible inhibitors for u03b1 chymotrypsin 34 j biol chem 250 15046 15053 1983 suicide inhibitors also called suicide substrates require catalytic activation to a form that irreversibly modifies the enzyme the compounds of the present invention improve on existing haloeonol lactone suicide inhibitors by being selective for ce one method for synthesizing compounds of the present invention is scheme 1 illustrated in fig1 an appropriately substituted diethylmalonate sodium salt 1a is heated with a cis 2 chloroacrylate 1b to give a triester compound 1c saponification and decarboxylation of the triester compound 1c produces a substituted glutaconic acid 1d which.