are made of synthetic or natural high polymer metal glass or ceramics etc 1 u00d7 10 4 b16 f10 a melanocyte are grown in the dmem medium with 10 fetal calf serum and incubated in a 5 co 2 incubator at 37 u00b0 c the bags filled with fir powder as the fir groups and the bags filled with non functional powder as the control group were inserted beneath the dishes of b16 f10 cells which are cultured with the cells without direct contact after 24 hrs and 48 hrs the mtt solution 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide is added into the cultured cells for a 4 hr incubation subsequently the suspension of the culturing is removed and the dmso dimethyl sulfoxide is added thereinto after a 10 minute incubation the absorbance of the cells is measured at 540 nm please refer to fig2 a and fig2 b which show the effect of the fir releasing substance of the present invention on the growth of pigment cells b16f10 fig2 a and fig2 b represent the results of cell growth co cultured with the fir releasing substance for 24 hrs and 48 hrs respectively the milk powder serves as a control group for comparing the difference between the control group and the fir group as fig2 a illustrates the.