is determined using the algorithm of karlin and altschul proc natl acad sci usa 87 2264 2268 1990 modified as in karlin and altschul proc natl acad sci usa 90 5873 5877 1993 such an algorithm is incorporated into the blast program of altschul et al j mol biol 215 403 410 1990 when utilizing blast and gapped blast programs the default parameters of the respective programs are used the reduction reaction may be carried out in a single phase system having the cells containing the enzyme suspended therein alternatively the reaction may be performed in a two phase aqueous organic solvent system as described in us 2009 0017510 and u s pat no 7 371 903 the reaction may be carried out as a conventional batch reaction or as a continuous process it will be appreciated that one of the significant advantages of the enantioselective enzymatic reduction for commercial applications is that it is amenable to continuous operation the reaction mixture preferably contains from about 35 g to 500 g of cells per kg of raw product produced by the reduction the suspension is the aqueous portion of the reaction mixture which also contains a buffer for example a tea triethanolamine phosphate tris hcl or glycine buffer the buffer may additionally comprise ions for the stabilization of the enzyme for example a source of magnesium ions additional additives that may be present in the buffer for stabilizing the enzymes may include a polyol such as glycerol sorbitols and the like sulfur compounds such as 1 4 dl dithiothreitol glutathione cysteine or the like amino acids and peptides or detergents such as dmso a preferred stabilizer for the enzyme is a polyol particularly glycerol which may be present in.