glass plate 302 is placed in front of the second lens 214 replacing the emission filter 216 of fig2 to obtain the same focus and image size as in fluorescence imaging the sample was illuminated with ambient light e g room lights 304 referring to fig4 two skin pieces 402 and 404 about 2 to 2 5 mm thick were placed free standing border to border on a translation stage the dermal layers are shown 406 as well as the subcutaneous tissue 408 laser tissue welding was performed on the region between the two pieces 410 by scanning the laser irradiation the stage was moved forward at approximately 5 mm 30 sec then back at the same speed at the same length a 5 mm fusion line was formed no conventional suture was performed the total exposure time was 5 mm min a cunyite cr 4 ca 2 geo 4 tunable laser 412 at 1 430 nm and a forsterite cr 4 m g2 sio 4 tunable laser 412 at 1 250 nm were used for laser tissue welding n 10 for each group the energy fluencies were 10 5 kj cm 2 and 19 1 kj cm 2 respectively the main mechanism of cr 4 laser forsterite and cunyite tissue welding uses the absorption.