e g mouse human etc are included in kit for calibration curves 3 32 p u03b1 atp over 3 ci mmol and 1 mci ml 7 alumina elution buffer 1 m glycine 0 75 n hcl ph 2 these reagents are provided in a kit as above paper electrophoresis and thin layer chromatography equipment needed is commonly available the amounts of reagent needed are provided invariable amounts according to the number of determination the user desires to perform table 1______________________________________interferon induced enzymes and antiviralstate in l cells protein phospho interferon kinase oligo isoadenylate diesteraseu ml vsvrna pki synthetase e 2 39 pdi______________________________________0 100 15 4 252 20 35 5 7210 5 80 20 8650 2 100 42 100250 0 661250 0 100 100______________________________________ results are in percent of maximum activity cells were treated for 8 hours with interferon enzyme assays are as described in the text measure of vesicular stomatitis virus vsv rna synthesis as in weissenbach et al european j biochem 197 98 1 8 table 2______________________________________kinetics of enzyme induction by interferontime after human fibroblasts interferon mouse l cells oligo isoadenylateaddition protein kinase pki synthetase______________________________________hours phosphorylation 2 39 5 39 oligo a synthesized of the 35 000 mr counts min subunit of eif2 arbitrary units0 15 9002 15 9004 30 1 5008 55 3 75010 75 6 50024 100 13 500______________________________________ with 200 units ml mouse interferon with 50 units ml human fibroblast interferon table 3______________________________________variations in the level ofoligo isoadenylate activity in humanwhite blood cells underdifferent pathological conditions oligo isoadenylate synthetase sup 32 p 2 39 5 39 oligo a blood.