peak area solvent solution acetonitrile water 1 1 mass spectra were obtained with electrospray ionisation and the instrument was run in a positive mode analytes have been detected in the protonated form compound of formula viii r 1 u2014 co nh 2 r 1 u2550 r 2 u2550 h pneumocandin b 0 2 02 g 1 90 mmol and triflic acid 3 0 ml 5 09 g 33 9 mmol were dissolved in pyridine 30 ml at rt the mixture was heated to 80 u00b0 c and stirred for 12 h in inert atmosphere after cooling to 0 u00b0 c 1 2 diaminoethane 1 00 ml 0 90 g 15 0 mmol was added and the mixture was stirred over night the reaction was quenched by adding the reaction mixture to a mixture of water 100 ml and acetic acid 21 ml 22 0 g 0 37 mol resulting in a solution with ph 5 0 the solution was loaded onto a 10 mm c18 chromatography column eluting with a gradient from 20 acetonitrile 80 water to 25 acetonitrile 75 water evaporation of the organic solvent and freeze drying of the rich cuts afforded 534 mg 22 yield of the title compound as the triflic acid addition salt purity hplc 87 8 1 h nmr 600 mhz cd 3 od u03b4 7 13 2h d j 8 6 hz 6 74 2h d j 8 6 hz 5 08 1h d j 4 0 hz 5 02 1h d j 3 5 hz 4 80 1h d j 1.