showed no response to the light stroking of the paintbrush the scores were summed to generate a cumulative score of 0 to 16 as described in minami et al pain 57 217 223 1994 sulprostone treated mice typically have a score of 14 and vehicle treated mice typically have a score of 5 6 chronic pain compounds including gabapentin and amitriptyline are active in this model consistent with effects in other chronic pain models and chronic pain patients as described in gil d w cheevers c v and donello j e br j pharmacol 153 769 74 2008 in addition the locomotor activity of 5 6 mice per group was measured in a five minute period 30 minutes following intraperitoneal dosing with compound i the activity is determined automatically by placing the mice in an exploratory chamber omnitech electronic that has photocell beams criss crossing the box that are interrupted as the animal moves around the computer analyses this movement and records total activity locomotor activity relative to vehicle treated animals was expressed as a percentage percentage sedation was calculated as 100 minus the percent locomotor activity statistical calculations of significance for in vivo studies were done using a two tailed student 39 s t test as shown in fig1 and 2 compound i administered at a dose of 1.