that of the native enzyme the incubation time was varied up to 3 hours for measuring the recovery of catalytic activity by the denatured cpa 260 u03bcl aliquots of the enzyme solution initial concentration 1 92 u00d7 10 u2212 4 m was added to 240 u03bcl buffer solution 0 5 m nacl and 0 05 m hepes at ph 7 5 followed by the addition of 200 u03bcl of tfe and 300 u03bcl water in the presence of different molar ratios of p188 at ambient temperature u02dc 24 u00b0 c the final concentration of the enzyme upon dilution was 5 u00d7 10 u2212 5 m in the solution mixture the concentration of p188 was varied so as to yield enzyme p188 mixtures in 1 10 1 5 1 2 1 1 1 0 5 and 1 0 3 molar ratios corresponding controls were set up in parallel by similar dilution of the enzyme but in the absence of p188 catalytic activity was then determined by addition of 10 u03bcl of the chemically denatured enzyme solution to a solution containing cicpl to initiate the reaction final concentrations of the substrate and enzyme were of 5 6 u00d7 10 u2212 5 m and 1 67 u00d7 10 u2212 7 m respectively fig3 shows the kinetics of catalytic activity determined after a 30 and 60 min incubation period of the diluted enzyme in the presence or absence of p188 in the absence of p188 the maximum recovered activity was equivalent to only 0 7v max observed for the native enzyme prior to denaturation in the presence of p188 the maximum recovered activity was observed for the enzyme p188 mixture of 1 1 the recovery process equivalent to 0 9v max observed for the native enzyme prior to denaturation was.