with the flow rate at 0 3 ml min a standard curve is prepared to include about 0 1 0 7 mg ml of the expected compound a concentration drug loading is determined as percent drug loading 100 u00d7 10 u00d7 concentration of filtered solution weight of microspheres used the results are reported as the mean u00b1 sd microsphere drug release experiments are carried out in 0 1 m phosphate buffer ph 7 4 containing 10 mm ascorbic acid microsphere suspensions containing known amounts of drug about 30 mg are placed in a regenerated cellulose membrane dialysis device float a lyzer the float a lyzer is placed in a 45 ml falcon tube containing 40 ml buffer the release medium maintained at 37 u00b0 c and shook horizontally at 100 rpm in an oven the buffer is replaced with fresh solution at predetermined time intervals the drug content of the release medium is determined via uplc using uv vis absorbance at 314 nm with a standard curve constructed with known concentrations of iti07 3 30 u03bcg ml for in vivo characterization in vivo drug release pharmacokinetic study is determined the pharmacokinetics of the formulation is studied in adult male sprague dawley rats rats n 12 experiment are injected subcutaneously in the intra scapular region with a suspension 2 ml kg of compound a free base formulated in biodegradable plga microspheres about 60 mg rat suspended in a solution of 0 5 low viscosity carboxymethylcellulose.