ken japan their mycological features are described in jp a 9 140390 and european patent no 0805211 the transformants of interest can be obtained according to the production methods disclosed in jp a 10 210984 and japanese patent application no 9 311046 culture of the microorganism used in the present invention can be performed using usual media which contain an assimilable carbon source e g glycerol glucose or saccharose nitrogen source e g inorganic ammonium salt or nitrate casamino acid meat extract yeast extract or corn steep liquor and essential ingredients for growth of each microorganism such as inorganic salts e g magnesium chloride calcium chloride manganese sulfate iron chloride and zinc sulfate vitamines and nucleic acids addition of maleic acid and s s edds to the culture medium is preferred because it can result in increased malate isomerase activity and eddsase activity the culture medium preferably has a ph of 4 to 10 preferably 6 to 9 the culture temperature ranges from 20 u00b0 c to 90 u00b0 c preferably from 25 u00b0 c to 80 u00b0 c culture can be conducted aerobically for 1 to 14 days 0 01 to 5 weight each or preferably 0 1 to 2 weight each of a microorganism cell having malate isomerase activity from which enzyme activity involving side reactions e g fumarase.