2 53 68 50 10 40 1947 11 2 53 29 50 45 50 922 11 6 47 9______________________________________ sup 1 signal plus noise was measured in the presence of octylglucoside detergent and noise was measured in the absence of octylglucoside correction was made for background fluorescence from the detergent and buffer as was the case with preparations described in example 1 mean diameter decreased as a function of vortexing time signal to noise trapped volume and capture increased as a function of vortexing time the trapped volume decreased and the capture increased at higher lipid concentrations the trapped volumes measured for vortexed liposomes prepared with 50 100 micron glass beads 10 4 20 9 liters mole were higher than reported by deamer d and uster p deamer et al 34 lipsomes 34 ch 1 liposome preparation methods and mechanisms marc j ostro ed m dekker inc pg 27 51 new york new york 1983 for multilamellar liposomes 2 7 liters mole the high capture at higher lipid concentrations together with the decreasing mean diameter at longer vortexing times is an advantage in potential drug delivery applications entrapping an enzyme in liposomes vortexed with 50 100 micron diameter glass beads a ten micromole lipid film 4 725 micromoles.