solutions 202 typically in the first vitrification step the media will include two cyroprotectorants which will each provide 7 5 of the first step medium then in the second vitrification step the cryoprotectorants will each provide 15 of the second step medium after they have been thus prepared for vitrification in the are then moved to storage in a liquid nitrogen tank 204 the specimens will remain in the tank 204 until needed to be thawed and used the method of this invention includes a specially designed media in the sequence to provide the embryos with much needed components before they enter the vitrification process the embryos are cultured in an embryo culture media 220 before cryopreservation the embryos are moved from the generic culture medium into a preparation rejuvenation medium 225 a which contains the combination of ingredients described above and is devoid of hepes or mops this special solution contains additional ingredients useful in preparing the specimen for cryopreservation the embryos are then moved into a second preparation rejuvenation medium 225 b which does contain hepes or mops including hepes or mops a bicarbonate allows the embryos to be handled outside the incubator environment and includingn hepes or mops allows the user to prepare the embryos inside of the incubator environment for a longer period of time the embryos are then placed in the sequential vitrification solutions 230 and 231 and then stored in liquid nitrogen tank 275 referring now to the tables table 1 shows the changes made to a basic media formulation a this table shows what is a generic medium solution that has.