ask1 fragment cloned in pbind were ask1a 1 1035 ask1b 959 2010 ask1c 1820 2712 ask1d 2581 3152 ask1e 3153 3552 and ask1 f 3478 4100 as shown in figure 1a the full length nef gene was cloned in pact mammalian two hybrid vector which has all the interacting domains accession no gq184335 the mammalian two hybrid vectors cloned with or without nef and ask1 fragments were co transfected along with g5luc luciferase vector in atcc crl 1573 u2122 cells the reporter luciferase expression was measured after 48 hours of transfection among the ask1 fragments ask1a ask1b and ask1d showed 2 28 1 10 and 2 89 fold respectively luciferase activity compared to negative control pact pbind ask1c ask1d and ask1f showed no luciferase activity compared to control as shown in fig 1 b the myod id protein was taken as positive control for showing interaction of proteins in mammalian teo hybrid model as shown in fig1 c in this model the set of vectors which is expressing luciferase gene in cells are considered to be interacting with each other our results show that ask1a ask1b and ask1d fragment in presence of nef express luciferase gene which shows that they interact with nef where as other fragments of ask1 does not show any tendency for interaction with nef in mammalian two hybrid model ask1a.