5fecl sub 3 0 02 34 v 8 34 juice 150 ml l ______________________________________ sup 1 nutrients were dissolved in distilled water and the solution was adjusted to ph 6 with 50 w v naoh seedlings for host range tests at stoneville miss were greenhouse grown in 5 5 cm square peat pots containing a commercial blend of peat pine bark and perlite with one seedling per pot aqueous suspensions were prepared comprising conidia of the eastern black nightshade isolate of c coccodes produced by culture on the half strength emerson 39 s yeast starch agar in 0 05 w v oxysorbic 20 poe polyoxyethylene sorbitan monooleate surfactant 34 tween 80 34 at a concentration of 1 u00d7 10 6 conidia ml the seedlings were inoculated by wetting the foliage with the suspension in the third to fourth leaf stage immediately following inoculation the plants were placed in dark dew chambers 25 u00b0 c for 24 hours then moved to greenhouse benches and evaluated for disease symptoms 14 days after inoculation susceptible plants displayed anthracnose symptoms necrotic lesions usually within 3 days following inoculation a randomized complete block design was used with three replicates consisting of 12 plants of each species per replicate the experiment was conducted twice the results are reported in table i below the procedure of example 2 was repeated except that the seedlings were inoculated by injecting approximately 20 u03bcl of the conidial suspension into the stems a randomized complete block design was used with three replicates consisting of 12 plants of each species per replicate the experiment was conducted twice the results are reported in table ii below tests were conducted at st paul minn to determine the reaction of weeds of.