relates to primers for identifying shigella flexneri serotypes comprising the sequences of seq id nos 1 and 2 seq id nos 3 and 4 seq id nos 5 and 6 seq id nos 7 and 8 seq id nos 9 and 10 seq id nos 11 and 12 seq id nos 13 and 14 seq id nos 15 and 16 these primers are specific and have a common annealing temperature the present invention further relates to a multiplex amplification based identification method using the primers the present invention further relates to the use of the primers for identifying shigella flexneri serotypes for the preparation of identification agents the present invention further relates to a kit for identifying shigella flexneri comprising the above primers application_number US 201214241833 A description the present invention will be further described with reference to the following examples and the figures for better understanding of the invention the examples are only illustrative and are not in any way limiting the scope of the present invention in the examples any experimental methods without detailed conditions specified are conventional methods with conventional conditions well known in the art or conditions recommended by the manufacturer strains 14 known shigella flexneri serotypes except serotype 5b see table 1 for details were used in the examples to establish the conditions for performing the multiplex pcr method 358 shigella flexneri strains in total table 2 were used to evaluate the effectiveness of the multiplex pcr method of the present invention in order to examine the cross reactions of the primers in this study 50 strains from different genera were examined s sonnei n 2 s dysenteriae n 12 including.