11 hz 1h 8 58 dd j 1 76 9 39 hz 1h 7 85 7 88 m 1h 7 62 d j 7 63 hz 1h 7 42 7 45 m 1h 7 27 s 2h 7 16 7 19 m 3h 4 79 br s 2h 4 20 br s 2h 4 00 t j 8 80 hz 2h 3 27 3 31 m 2h 2 68 t j 7 63 hz 2h 2 55 t j 7 04 hz 2h 1 84 quin j 7 63 hz 2h 1 69 1 74 m 2h novel compounds were synthesized and tested for s1p1 activity using the gtp u03b3 35 s binding assay these compounds may be assessed for their ability to activate or block activation of the human s1p1 receptor in cells stably expressing the s1p1 receptor gtp u03b3 35 s binding was measured in the medium containing mm hepes 25 ph 7 4 mgcl 2 10 nacl 100 dithitothreitol 0 5 digitonin 0 003 0 2 nm gtp u03b3 35 s and 5 u03bcg membrane protein in a volume of 150 u03bcl test compounds were included in the concentration range from 0 08 to 5 000 nm unless indicated otherwise membranes were incubated with 100 u03bcm 5 u2032 adenylylimmidodiphosphate for 30 min.