to be gt 0 4 ng ml with cv 39 s in the order of 12 4 the polyclonal antisera was raised in guinea pigs with a pst peptide antigen the ria results table 1 indicate that the pst gene construct produced protein fig2 which is recognised by polyclonal antisera raised against the native form of pst purified from porcine pituitary glands l6 clones pci pst 1 5 were generated from the modified transfection technique as described below characteristically l6 cells adhere to culture plates and require detachment with trypsin to passage cells transfection is routinely performed 24 hrs later this procedure resulted in l6 cell clones n 10 secreting pst at 6 18 ng ml applying lipotaxi promega and the iss pst plasmid to the l6 cells 2 hrs after trypsin treatment increased the secretion rate of pst 10 20 fold gt 180ng ml n 5 clones this higher pst secretion rates reduce the number of cells capsules required to enhance growth encapsulation of cells at room temperature utilises calcium chloride or lactate 100 mm to gel the alginate 1 5 w v droplets followed immediately by washing with saline 0 9 naci then resuspending in poly l lysine 0 05 for 5 min calcium chloride crosslinking for 10 min at 37 u00b0 c resulted in an alginate matrix that was more compatible with cell viablity after the poly l lysine coating and saline washes another alginate layer is added sodium citrate 55 mm treatment for 4 min at room temperature softens the capsule to a consistency that increases the difficulty of further manipulation cell viablity is apparently reduced.