where the absolute levels of modified peptides can be determined in the same manner as determining absolute levels of unmodified peptides a perform relative and or absolute quantitation of fragment peptide levels of the bim protein and demonstrate that the previously determined association as well understood in the field of cancer of bim protein expression to the stage grade status of cancer in patient or subject tumor tissue is confirmed b perform relative and or absolute quantitation of fragment peptide levels of the bim protein and demonstrate correlation with clinical outcomes from different treatment strategies wherein this correlation has already been demonstrated in the field or can be demonstrated in the future through correlation studies across cohorts of patients or subjects and tissue from those patients or subjects once either previously established correlations or correlations derived in the future are confirmed by this assay then the assay method can be used to determine optimal treatment strategy a srm mrm assay to determine the amount of the fragment peptide s of the bim protein s that is are detected to determine the relative and or absolute amount of bim protein s in a protein lysate 1 determining increased or decreased presence of bim protein s by comparing the srm mrm signature peak area from one or more two or more or three or more given bim peptides detected in a liquid tissue u2122 lysate from one formalin fixed biological sample to the same srm mrm signature peak area of the same bim fragment peptide s in at least a second third fourth or more liquid tissue u2122 lysates from least a second third fourth or more formalin fixed biological samples 2 determining increased or decreased presence of the bim protein s by comparing the srm mrm signature.