asp39 are the catalytic residues fig1 b only the catalytic domain residue numbering is used the first residue is ala and the second one is thr seq id no 14 moreover the structural analysis showed that gly103 ser104 gly105 ser106 leu131 gly132 gly133 gly134 ala156 gly158 asn159 ser221 gly222 thr223 and met225 are involved in substrate interaction fig1 b the sequences shown in fig1 b 11b are produced from yeast system the structural analyses were performed using keratinase produced by e coli expression system the linker sequence used in yeast system expression system was gnlyfqs seq id no 16 in e coli system the residue g was replaced by e the foregoing description of the exemplary embodiments of the invention has been presented only for the purposes of illustration and description and is not intended to be exhaustive or to limit the invention to the precise forms disclosed many modifications and variations are possible in light of the above teaching the embodiments and examples were chosen and described in order to explain the principles of the invention and their practical application so as to enable others skilled in the art to utilize the invention and various embodiments and with various modifications as are suited to the particular use contemplated alternative embodiments will become apparent.