one at different concentrations 125 100 um for 1 hour and then were stimulated with tpa 25 ng ml after 3 hours the whole cell extracts were prepared and subjected to emsa electrophoretic mobility shift assay as described for nf u03bab in u zabel et al j biol chem 266 242 1991 and hsf in c amici et al cancer res 55 4452 1995 to determine nf u03bab and hsf activation respectively the positions of the complex nf u03bab dna nf u03bab and the non specific binding ns are indicated in fig1 a the positions of the complex hsf dna hsf the hsf dna constitutive activity chba and the proteins dna non specific interactions ns are indicated in fig1 b the line u201c control u201d indicates the non tpa stimulated cells as a control of non activated nf u03bab as evident 2 cyclopenten 1 one is able to inhibit nf u03bab activation by tpa even at the lower concentration of 125 um at the concentration of 500 um the nf u03bab band is absent fig a in correlation with nf u03bab inhibition in the same samples it is evident the activation of hsf starting from the concentration of 125 um fig1 b the cells were treated for 1 hour with the same concentration 500 um of 2 cyclopenten 1 one line 3 cyclopentanone line 4 or cyclopentene line 5 and then were stimulated with tpa 25 ng ml c represents the non tpa stimulated control after 3 hours the whole cell extracts were prepared and subjected to emsa to verify the activation of nf u03bab fig1 c and of hsf fig1 d respectively as evident i tpa activates nf u03bab line 2 ii 2 cyclopenten 1 one inhibits tpa induced nf u03bab activation line 3 cyclopentanone line 4 and cyclopentene.