water and then with a saturated aqueous solution of sodium chloride and then dried over anhydrous sodium sulfate during continuous addition of methanol the solution was then condensed by evaporation under reduced pressure using a rotary evaporator to obtain 10 ml of an oily substance this oily substance was dissolved in 100 ml of 60 v v aqueous methanol and the resulting solution was adsorbed on sep pak vac 20 cc c 18 cartridges a trade name for a product of waters co usa impurities were eluted with 30 ml of 60 v v aqueous methanol the leustroducsins were then eluted with 15 ml of 100 methanol and the eluate was condensed to obtain 800 mg of an oily substance this oily substance was used directly and without further purification in example 1 above in order to purify the material this oily substance was dissolved in 10 ml of methanol and subjected to high performance liquid chromatography the fractions showing peaks near 13 minutes 19 minutes and 24 minutes were collected and are referred to as 34 raw fraction a 34 34 raw fraction b 34 and 34 raw fraction c 34 respectively the conditions used for the chromatography are shown below eluting solvent 50 by volume aqueous acetonitrile containing.