amino acid from either end is unique the human genome contains the genes that encode all proteins the proteolytic cut sites within all these proteins can be predicted from the translated amino acid sequence the mass of the peptides that result from the predicting cut sites can be calculated similarly the fragmentation pattern from each hypothetical peptide can be predicted thus we can conceptually digest the proteins within the human proteome and fragment them when a peptide has been u201c sequenced u201d it is understood that the peptide fragment has been purified by one of the methods above i e time of flight tof or by chromatography before fragmenting it with gas to produce the peptide fragments the original peptide mass and fragmentation pattern obtained is then fit to those from the theoretical digestion and fragmentation of the genome the peptide that best matches the theoretical peptides and fragments and is biologically possible i e a potential human blood borne protein is thus identified it is possible to identify plural targets in this fashion following are exemplary but non limiting examples of preparatory protocols useful in the process of the instant invention any of these protocols may be selected from a column flow through.