reported as the mean of triplicate determinations fig3 shows the inhibition of calcein release by 3 deoxyflavonoids luteolin and tricetin the effect of luteolin treatment in chronic type i bbwor diabetic rats was examined in this study lean male diabetic rats were randomly assigned to 3 treatment groups 3 4 rats group each group received either 1 3 mg dose of luteolin intragastrically 2 a subcutaneous injection of pzi insulin 0 9 1 2 mu day or 3 no treatment blood glucose was evaluated from time 0 through 6 hours the data was expressed as average blood glucose relative to time post treatment the rats that received a single injection of insulin showed a 75 decrease in blood glucose levels 415 to 112 mg dl within 6 hours of injection this response was fully consistent with our prior work in the type 1 bbwor rat model rather remarkably diabetic rats that received luteolin showed a 31 drop in blood glucose levels 445 to 307 mg dl in 6 hours in comparison there was no reduction in the hyperglycemic state in the control group over the same interval 414 to 404 mg dl thus a single 3 mg dose of luteolin was able to reduce hyperglycemia within 6 hours as much as 31 in insulin dependent diabetic type i bbwor rats luteolin was also evaluated for its effect to reduce hyperglycemia in chronic type 2 diabetic rats in this study the dose and frequency of luteolin treatment was increased to compensate for the enhanced metabolism of.