8202 aminco bowman spectrophotofluorometer slm instru inc urbana ill equipped with a 9 u03bcl continuous flow cell separations were made on either a 4 6 mm analytical or 1 0 cm semi preparative u00d7 25 cm vydac 218tp 10 micron c 18 column the separations group hesperica calif by application of a linear gradient system of 0 17 acetonitrile from 10 to 97 min with either tfa or hfba as counterion at a flow rate of 1 analytical or 2 ml min semi preparative pyridoxamine sigma chem co st louis mo which autofluoresces was used as an internal standard for analytical purpose the hplc program was shortened such that a linear gradient of 10 17 acetonitrile was applied from 0 35 min with hfba as the counterion see fig5 sugars were incubated with l lysine and l arginine at 80 u00b0 c for 1 hour in pbs each sugar and amino acid were present at concentrations of 100 mm in a total volume of 2 ml in 13 u00d7 100 mm test tubes placed in a reacti therm heating block pierce chem co rockford ill a pool of young dura mater average age 15 years obtained at autopsy was homogenized in pbs and extracted for 24 hours in 2 1 chloroform methanol dry blotted tissue 0 5 g wet weight was incubated with 100 mm each of the pentose sugars l arginine and l lysine in 12 ml of pbs containing 10 u03bcl each of toluene and chloroform after incubation at 37 u00b0 c for 6 days 0 3 g wet tissue weight was withdrawn from each tube and washed three times with 5 ml portions of pbs and water respectively samples were acid hydrolyzed in 2 ml of 6 n hcl for 24.