the route of administration is intraperitoneal without wishing to be bound by theory it is thought that intraperitoneal administration actively and effectively recruits intraperitoneal macrophages that travel to the pancreas to provide prophylaxis and treatment of pancreatitis the studies presented elsewhere herein indicate that cd11b mac 1 does not play a role in the migration of ho 1 cells to the pancreas and the increased cells seen with the treatment may reflect the rise in circulating monocytes in addition ccr2 may be important for trafficking of u201c protective u201d monocyte macrophage into pancreas since in its absence potential u201c pro inflammatory u201d leukocyte may have advantage over the u201c protective u201d monocyte macrophage to migrate into the pancreas and cause injury hemin vehicle and antibodies hemin sigma st louis mo was dissolved in 10 ammonium hydroxide in 0 15 m nacl to prepare a stock solution of 100 mg ml then further diluted 1 40 with sterile 0 15 m nacl and injected into mice 10 u03bcl g vehicle injected mice received an identical nh 4 oh containing solution lacking hemin the antibodies used were directed to ho 1 and ho 2 stressgen victoria canada vimentin smooth muscle actin and luciferase neomarkers fremont calif allophycocyanin conjugated gr 1 and mac 1 bd pharmingen san diego calif and fitc conjugated f4 80 serotec raleigh n c panhematin was obtained from ovation pharmaceuticals inc deerfield ill mice and pancreatitis models experiments and animal care were.