above they were then applied by the insertion method described above to healthy male fertile plants of gossypium hirsutum as an additional test to demonstrate the effectiveness of this technique untreated and buffer only controls were also sprayed onto plants to quantify enviromental sterility and sterility caused by physical damage to plants during the insertion process beginning twenty four days after treatment and continuing for six days during the peak bloom cycle of the test plants the frequency of complete and partial sterility within the treated population averaged several times greater than in either control treatment the data are summarized in the following table which demonstrates statistically the efficacy of this method numbers indicate the number of sterile blooms observed among samples of 250 plants for each control group during days 24 29 a significantly larger than average number of sterile blooms were observed indicating that despite some plant damage and mortality as well as environmental factors and natural occurrence of the cytoplasmic male sterility trait the method of the present invention is effective in transforming cells in the target plants overall the previously described method of the present invention shows substantial advantages over traditional methods of both nuclear and organelle based transformations this version is cheap cost effective and requires little specialized equipment outside that ordinarily found in a general laboratory furthermore this version of the present invention produces rapid results with the transgene beginning to express within the time span of.