studies test substance is administered p o in olive oil 1 u00d7 daily for 3 days or in powder form in methylcellulose once prior to oa challenge on day 3 test animals receive test substance 1 5 hrs prior to and 6 hrs after oa challenge for inhalation studies test substance is micronised for delivery to test animals restrained within a flow past nose only inhalation chamber administration by inhalation is effected 15 mins prior to oa challenge efficacy of administered test substance is determined by bronchoalveolar lavage bal and cell counting for this purpose animals are sacrificed with na pento barbitone 100 mg kg i p and the trachea is exposed and cannulated 5 successive 10 ml aliqots of ca 2 and mg 2 free hank 39 s balanced salt solution hbss containing bovine serum albumin bsa 0 3 edta 10 mm and 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes 10 mm is then introduced into the lung and immediately aspirated by gentle compression of the lung tissue total cell counts in pooled eluates are determined using an automatic cell counter lavage fluid is centrifuged at 200 g for 10 minutes and the cell pellet resuspended in 1 ml of supplemented hbss 10 u03bcl of this cell suspension is added to 190 u03bcl of turk 39 s solution 1 20 dilution differential cell counts are made from smears stained by diff quick cells are identified and counted under oil immersion u00d7 1 000 a minimum of 500 cells per smear are counted and the total population of each cell type is calculated although the invention has been.