then heated at 100 u00b0 c or 30 minutes hydrolysis mixtures were diluted with water 1 9 and filtered millex hv 0 45 u03bc prior to hplc analysis separation of flower pigments was effected via gradient elution using the following system solvent a triethylamine conc h 3 po 4 h 2 o 3 2 5 1000 detection mwd with simultaneous data acquisition at 280 350 and 546 nm the anthocyanidin peaks were identified by reference to known standards an alternative method for the analysis of anthocyanin molecules present in petal extracts is to be found in brugliera et al 1994 hplc analysis is conducted to determine the presence of delphinidin pelargonidin and cyanidin pigments in samples of carnation chrysanthemum and rose tissues having been transformed with one or other of the plasmids pcgp90 pcgp485 pcgp484 pcgp628 pcgp653 or pcgp1458 representative data of pcgp90 pcgp485 and pcgp653 in transgenic carnation flowers are shown in table 1 table 1______________________________________hplc analysis of pcgp90 pcgp485 and pcgp653 transgenic flowers del pelar sample phinidin gonidin cyanidin______________________________________non transgenic carnation cultivar red sim 0 85 3 0 8transgenic carnation red sim pcgp90 i acc 1933 1 9 82 7 nd ii acc 2011 3 7 76 9 ndred sim pcgp485 i acc 3654b 13 0 75 1 2 3red sim pcgp653 i acc 3660 2 18 1 71 4 3 2 ii acc 3655 35 6 49 1 7 5______________________________________ acc plant accession number nd not detected.