agcl in actual measurement cholesterol solution of strength varying between 0 5 10 mm in a phosphate buffer of ph of 7 0 was used with the two electrodes as described above the current due to enzymetrically produced h 2 o 2 was measured every 100 seconds typically a response time of seconds was measured for a concentration of the reaction giving rise to current is due to the following scheme the results of the experiments are shown in fig1 in order to check if addition of any interfering agents in cholesterol like glucose or ascorbic acid will have any deleterious effect on the response of the enzyme electrode the experiment was repeated with cholesterol solution mixed with the interfering agents it was found that these interfering agents did not show any effect on the response to the enzyme electrode in an attempt to improve the shortcomings of the prior art disclosures of cholesterol measurement the bio molecules are immobilized in sol gel and have comparatively enhanced shelf lives this is because of fact that i a variety of enzymes may be encapsulated in sol gel matrices giving optical transparent glasses ii the enzymes are remarkable stable in such matrices iii these enzymes undergo characteristics reversible reaction in sol gel glasses and iv spectroscopic changes occurring in sol gel glasses can readily be quantified by optical spectroscopy the sol gel technique is advantageous since little or no heating is required such enzyme molecules become.