100 mg naa was weighed and dissolved in 1 ml 1 n potassium hydroxide for 5 minutes then 10 ml distilled water was added for complete dissolution the resulting solution was brought to 100 ml with distilled water and stored at 4 u00b0 c for later use 100 mg iaa was weighed and dissolved in 1 ml 1 n potassium hydroxide for 5 minutes then 10 ml distilled water was added for complete dissolution the resulting solution was brought to 100 ml with distilled water 125 g glucose was weighed and dissolved with distilled water the resulting solution was brought to 250 ml with distilled water sterilized and stored at 4 u00b0 c for later use 0 392 g as and 10 ml dmso were charged into a 1 5 ml centrifuge tube and stored at 4 u00b0 c for later use 5 6 g potassium hydroxide was weighed and dissolved in distilled water the resulting solution was brought to 100 ml with distilled water and stored at room temperature for later use 3 components and amounts of culture media for genetic transformation of rice distilled water was added to a volume of 900 ml and the ph value was adjusted to 5 9 with 1 n potassium hydroxide the resulting mixture was boiled and brought to 1000 ml the resulting medium was dispensed into 50 ml erlenmeyer flasks 25 ml flask and the flasks were sealed and sterilized at 121 u00b0 c for 12 minutes distilled water was added to a volume of 900 ml and the ph value was adjusted to 5 9 with 1 n potassium hydroxide the resulting mixture was boiled and brought to 1000 ml the resulting medium was dispensed into 50 ml erlenmeyer flasks 25 ml flask and the flasks were sealed.