the compositions and or methods disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure while the compositions and methods of this invention have been described in terms of preferred embodiments it will be apparent to those of skill in the art that variations may be applied to the compositions and or methods and in the steps or in the sequence of steps of the methods described herein without departing from the concept spirit and scope of the invention more specifically it will be apparent that certain agents that are both chemically and physiologically related may be substituted for the agents described herein while the same or similar results would be achieved all such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit scope and concept of the invention as defined by the appended claims the following references to the extent that they provide exemplary procedural or other details supplementary to those set forth herein are specifically incorporated herein by reference u s pat no 7 135 613 u s pat no 7 700 825 u s pat no 7 834 239 brem et al aquaculture 68 209 219 1988 chourrout et al aquaculture 51 143 150 1986 delvin et al nature 371 209 210 1994 draper and moens in the zebrafish book 5 th ed eugene university of oregon press 2007 du et al bio technology 10 176 181 1992 gross et al aquaculature 103 253 273 1992 khoo et al aquaculture.