of boc proamc boc asn was added along with coupling agent noc asn proamc was obtained repeat same process glp was coupled to the sequence since no protection was employed hf cleavage step was avoided however if boc asn xan had been used hf step would have been necessary after the hf cleavage extract the acn h 2 o crude peptide is purified with rp hplc preparative hplc shimadzu 8 lc analytical hplc shimadzu 10 lc analytical column ymc 5 micron c18 preparative column 3 inch varian 10 micron c18 rp hplc collect those fraction gt 95 dry it over virtis lyophilizer and white powder was obtained with good yield the material was finally tested and released by qc with that parameter specified in coa glp asn proamc prepared by this process exhibited the correct molecular weight in mass spectral analysis it had a solubility of 0 5 mg in 0 5 ml water derivatives of this type have been previously synthesised by well known solution solid phase procedures using boc chemistry zimmerman et al 1977 30 fujiwara amp tsuru 1978 31 glp asn proamc and other compounds of the invention can be prepared utilising such procedures which are readily understood by those of ordinary skill in.