antisense rna under the control of the fruit enhanced tomato polygalacturonase pg promoter this vector includes approximately 5 kb of promoter sequence and 1 8 kb of 3 39 sequence from the pg promoter separated by a multiple cloning site after synthesis the vectors with sense orientation of ptom41 sequence are identified by dna sequence analysis a the vector pjr141a is constructed using a sequence corresponding to a fragment of the insert of ptom41 synthesised by polymerase chain reaction using synthetic primers the ends of the fragment are made flush with t4 polymerase and it is cloned into the vector pjr1 which has previously been cut with smai pjr1 smith et al 1988 nature 334 724 726 is a bin19 bevan 1984 nucleic acids research 12 8711 8721 based vector which permits the expression of the antisense rna under the control of the camv 35s promoter this vector includes a nopaline synthase nos 3 39 end termination sequence after synthesis of the vector the structure and antisense orientation of the sequences are confirmed by dna sequence analysis b another vector is constructed using sequences corresponding to a restriction fragment obtained from ptom41 and is cloned into the vectors ga643 an et al 1988 plant molecular biology manual a3 1 19 or pdh51 pietrzak et al 1986 nucleic acids research 14 5875 5869 which have previously been out with a compatible restriction enzyme s a restriction fragment from the ptom41 pdh51 clone containing the promoter the.