were left at room temperature for 24 h in the dark the assay plates were read by using an envision perkinelmer reader with 505 dichroic mirrors with 485 nm excitation and 530 nm emission settings and the plate reads were from the bottom by following the above protocol ec 50 dd2 results for compounds 1 150 are shown in table 2 below strains of plasmodium falciparum dd2 3d7 d6 k1 nf54 v1 3 hb3 7g8 fcb tm90c2b were obtained from malaria research and reference reagent resource center mr4 p falciparum isolates were maintained with o positive human blood in an atmosphere of 93 n 2 4 co 2 3 o 2 at 37 u00b0 c in complete culturing medium 10 4 g l rpmi 1640 5 94 g l hepes 5 g l albumax ii 50 mg l hypoxanthine 2 1 g l sodium bicarbonate 10 human serum and 43 mg l gentamicin parasites were cultured in medium until the parasitemia reached 3 8 parasitemia was determined by checking at least 500 red blood cells from a giemsa stained blood smear for the compound screening a parasite suspension at 2 0 parasitemia and 2 0 hematocrit was created with medium.