chloride to obtain the desired mechanical characteristics after sterilization the polymer was stored in a desiccator until ready for use for each fascial sling about 32 confluent 25 cm plates of each cell type collagen secreting cells elastin secreting cells and fibroblast cells were processed for coating onto the polyglycolic acid matrix the cells were resuspended in culture medium and applied at a cell density of about 107 cells ml to one surface of the polymer matrix the coated polymer was incubated in dulbeccos 39 s modified eagles medium dmem sigma st louis mo supplemented with 10 fetal calf serum biowhittaker inc walkersville md the medium was changed at 12 hour intervals to ensure sufficient supply of nutrients the cells were cultured until they attached to the surface of the polymer and began to grow and develop a second suspension collagen secreting cells was then coated onto the existing collagen layer the cells were incubated until they grew and developed into a layer of collagen cells the process was repeated until a polylayer of collagen developed the elastin secreting cell population was coated onto the collagen polylayer the cells were incubated until they formed an interface with the collagen polylayer and developed into a monolayer of elastin cells a second suspension of elastin secreting cells was then applied to the elastin monolayer and allowed to develop into a second monolayer the process was repeated until a polylayer of elastin cells developed over the polylayer of collagen.