methylmorpholine 0 1 g and anhydrous dmf 2 ml to the residue stir 12 hours and evaporate the solvent suspend the residue in etoac and wash successively with saturated acueous nahco 3 10 citric acid and water dry the organic layer over mgso 4 filter and evaporate the solvent to obtain the title compound r f silica gel ch 2 cl 2 ch 3 oh 95 5 0 87 add 6m hcl in dioxane 10 ml to the product of part e 6 g stir 10 minutes and evaporate excess acid and solvent add to this residue n u03b1 t butyloxycarbonyl asparagine 0 23 g dec 0 19 g 1 hydroxybenzotriazole 0 15 g n methylmorpholine 0 1 g and 2 ml anhydrous dmf stir 12 hours and evaporate solvent triturate the residue with water 10 ml and collect the product by filtration wash with diethylether 3 u00d7 20 ml to obtain the title compound r f silica gel ch 2 cl 2 ch 3 oh 95 5 0 60 add 6m hcl in dioxane 10 ml to the product of part f 0 75 q stir 10 minutes and evaporate excess acid and solvent add to this residue n u03b1 t butyloxycarbonyl serine 0 2 g dec 0 19 g 1 hydroxybenzotriazole 0 15 g n methylmorpholine 0 1 g and 2 ml anhydrous dmf stir 12 hours and evaporate solvent triturate the residue with water 10 ml and collect the product by filtration wash with diethyl ether 3 u00d7 20 ml and dry the product in vacuo add 6m hcl in dioxane 10 ml stir 10 minutes and evaporate the solvent purify on reverse phase hplc c 18 column ch 3 cn h 2 o cf 3 cooh 399 599 2 add 1n acueous.