these flasks are used to inoculate the laboratory fermentors p shermanii was stored in sodium lactate stabs 10 g trypticase 10 g yeast extract 10 g na lactate 0 25 g k 2 hpo 4 12 g agar one 1 deionized water 50 ml 25 mm u00d7 150 mm screw capped tube stabs were inoculated incubated at 30 u00b0 c for 72 hours and stored at 4 u00b0 c for up to 6 months a stab was revived by overlaying with 10 ml hansen 39 s glucose broth hgb 20 g trypticase 5 g yeast extract 5 g glucose one 1 deionized water and incubating for 48 hours at 30 u00b0 c a shake flask containing 2 teklac medium was inoculated with 10 ml hgb from one revived stab p shermanii the flasks were incubated for 48 hours these cultures were then used to inoculate production fermentors medium for preparation of lactic acid producing bacteria and operation of the 14 liter laboratory fermentor fresh pasteurized whey at approximately 6 5 total solids t s was the medium used for the inoculum build up prior to use the microfirm glass jar fermentor new brunswick scientific edison n j was sterilized by autoclaving at 250 u00b0 f for 30 minutes twelve liters of pasteurized whey was inoculated with lactic acid.