physiological saline solution preferably the washing procedure is repeated three times using a 0 85 nacl solution and centrifugation the washed precipitate is then lysed as by vigorous mixing with a preferably equal volume of distilled water for approximately two minutes the resultant lysate is predominately a hemolysate although containing small amounts of other proteins lipids and cell membrane remnants the lysate may be further treated by mixing with a solvent preferably a 0 2 volume of ccl 4 carbon tetrachloride is vigorously mixed with one volume of the lysate then set aside for thirty minutes at room temperature and again finally centrifuged the resultant supernate after dilution is the test sample 10 of a red blood cell hemolysate solution for separation detection and measurement of the amount of a group of hemoglobin species present according to the invention a 10 b procedure will include collecting a relatively small volume whole blood sample as by use of a microhematocrit tube one volume of a whole blood sample is mixed with four volumes of water preferably one drop of whole blood and four drops of distilled water are vigorously shaken together in a small test tube then the test tube is set aside for five minutes at room temperature after another and final mixing the resultant suspension without dilution includes a test sample.