in this example two layers of white pigmented 40 hex tredegar apertured film including 5 masterbatch with wpa silver glass antimicrobial were utilized and the edges were thermally sealed with heat pressure this film offers a one way flow feature and the flow is inward into the absorbent pad the testing conditions and protocol denoted in detail in example 7 was also used to test the performance of the film which though is 40 hex tredegar film in the test can be manufactured from pla as mentioned in example 5 we measured the bacterial count translated to 449 460 000 4 5 u00d7 10 8 total colonies per box on average this was a large number but there is a very large surface area of lettuce and the concentrate per leaf is really quite low the risk however was the potential for explosive growth with a strain that is pathogenic and can cause illness or death with certain bacteria it can reportedly and is known to those of ordinary skill in the art take as few as 100 colonies to cause illness in humans 1 that the 500 g of lettuce sampled from the top layer is representative of the entire box 2 that a u201c worst case u201d is for 100 of those bacterial colonies to become exposed to the packaging materials 3 that the e coli strain selected atcc 8739 is representative of bacterial contamination found on the lettuce leaves 4 that the distribution of colonies is uniform and not highly concentrated a known number of colonies and a certain area were used in the u201c treated u201d materials with which to control these colonies 0 495 square.