plates three plates were used for three different incubation periods of 3 6 and 12 h the wells in each plate were divided into groups for corresponding samples 1 positive control group repeating 3 wells only test cells were added 2 grgds containing hydrogel group composed of three sub groups pcl3k peg2k grgds 85 15 pcl3k peg2k grgds 70 30 and pcl2k peg2k grgds 85 15 hydrogel films repeating 3 wells for each group and 3 negative control group further divided into three sub groups 3 wells for each group corresponding to pure pcl3k network film pcl3k mpeg2k 85 15 and pcl3k mpeg2k 70 30 hydrogel film in each well the fibroblasts suspension was seeded on the top of each sample at a density of 3 1 u00d7 10 4 cells cm 2 cell cultures were maintained under standard culture conditions for different specific times at each time point the cells on the sample surface were rinsed with pbs twice to remove non adherent cells and lifted using 50 u03bcl well of 0 25 trypsin edta solution then the number of fibroblasts attached to the films was counted using hemacytometer total cell number at each time point was divided by the number of initially seeded cells to normalize the cell attachment data the experiment was performed 5 times for n 5 independent comparison the long time cell attachment study was carried out for three continuous days by observing the cell morphology and viability at different time points specifically the aforementioned sterilized samples pure pcl3k network film pcl3k mpeg2k.