time the patient may also be supplied with a home use version of the oxidizing composition and the sealant as an option for continued improvement in tooth color the above steps were performed on extracted molars and premolars n 25 obtained through orthodontists with patient consent and stored refrigerated in phosphate buffered saline pbs solution at ph 6 8 until use individual teeth were removed from the pbs solution allowed to air dry for 60 seconds and the roots inserted up to the cementoenamel junction into a high viscosity aqueous gel to keep the roots hydrated during the procedure an initial tooth shade was taken using a minolta cm504i chromameter konica minolta and recorded steps 2 total treatment time of 10 minutes and 3 total treatment time of 120 seconds were performed on the extracted teeth and a 32 minute period was allowed to elapse during which the teeth were rinse with water every 8 minutes to simulate the rinsing process that typically occurs during the cleaning process after the simulated cleaning process time had elapsed the teeth were polished with a medium grit prophy paste using a slow speed handpiece and prophy cup teeth were rinse with water and a final tooth shade was taken using the method described above and recorded in table 2 below l a b initial color readings l a b final color readings the following whitening method was used to demonstrate the ability of a high viscosity tooth whitening composition to remove an artificial stain from the surface of a bovine enamel substrate in vitro when light energy is use to enhance penetration a 10.