added after incubation was carried out in a predetermined time the degree of color developing from spots was observed by a microscope as a result of the analysis it was found that both trk 62 and trk 49 recognize epitopes near the binding sites of a sugar chain specifically present in tracp 5b it was considered that specificity of a reaction with tracp 5b is enhanced by this fig8 in consideration of the above results epitopes of trk 126 and trk 127 were analyzed by the luminescent method more specifically the peptide slides were subjected to a blocking treatment using superblock pierce at room temperature for 60 minutes thereafter a primary antibody i e trk 126 or trk 127 a concentration of 1 u03bcg ml was allowed to react at room temperature for 60 minutes after the slides were washed three times with pbs t a secondary antibody attached to amersham ecl prime ge healthcare was added and a reaction was carried out at room temperature for 60 minutes the slides were washed three times with pbs t again a detection reagent attached to a kit was added after incubation was carried out for a predetermined time the degree of luminescent from spots was checked by imagequant las4000 ge healthcare as a result of the.