the target u201c marker u201d is the term used herein to denote a gene or sequence whose presence or absence conveys a detectable phenotype of the organism various types of markers include but are not limited to selection markers screening markers and molecular markers selection markers are usually genes that can be expressed to convey a phenotype that makes the organism resistant or susceptible to a specific set of conditions screening markers convey a phenotype that is a readily observable and a distinguishable trait molecular markers are sequence features that can be uniquely identified by oligonucleotide or antibody probing for example rflp restriction fragment length polymorphism ssr markers simple sequence repeat epitope tags and the like the term u201c isolated u201d refers to protein s polypeptide s nucleic acid s oligonucleotide s factor s cell or cells which are not associated with one or more protein s polypeptide s nucleic acid s oligonucleotide s factors cells or one or more cellular components that are associated with the protein s polypeptide s nucleic acid s oligonucleotide s factor s cell or cells in vivo an u201c effective amount u201d generally means an amount that provides the desired local or systemic effect and or performance as used herein u201c fragments u201d u201c analogues u201d or u201c derivatives u201d of the polypeptides nucleotides described include those polypeptides nucleotides in which one or more of the amino acid residues are substituted with a conserved or non conserved amino acid residue and which may be natural or unnatural in one embodiment variant derivatives and analogues of.