at reducing erythema the dose response kinetics indicated that between 0 03 and 0 3 ppm the relationship between dose and response was linear maximum response was obtained at gt 0 3 ppm of avenanthramide further to example 2 the permeate 270 ml was evaporated under reduced pressure and made up to 10 mls in 1 1 v v aqueous ethanol the solution was applied to a lh 20 column 100 ml pre equilibrated in ethanol water acetic acid 40 59 1 the column was washed with 2 vb of solvent and the resulting fraction discarded the avenanthramides were eluted from the column with two bed volumes of 80 aqueous acetone the sample was evaporated to dryness under reduced pressure and then redissolved in 100 mls of 90 aqueous butylene glycol the solution was filtered through a 0 45 u03bcm filter whatman inc before packaging the finished isolated avenanthramide fraction contained 15 ppm of total avenanthramide the results of testing the isolated avenanthramide fraction oat extract and untreated control are shown in table 5 high performance liquid chromatography hplc for total avenanthramides was conducted using a beckman binary solvent delivery system using 32 karat u2122 analytical software for microsoft windows nt u2122 beckman coulter inc avenanthramides were separated on a csc ods hypersil u2122 column 250 u00d7 4 6 mm 120 u00e5 3 u03bcm using a c18 guard column supelco sigma aldrich corporation at 22c a beckman photodiode array pda detector monitoring.