acid concentrations above 1 resulted in almost complete hydrolysis after two hours a concentration of 1 needed three hours to complete the hydrolysis and 0 5 was not finished until after six hours at 100 u00b0 c the hydrolysis was completed in one hour for all acid concentrations hydrolysis products were formed in yields 95 100 based on the sucrose hydrolyzed with less degradation of fructose to produce a media with high enough sugar concentration for fermentation purposes higher liquid solid ratios higher percentages of sucrose must be used using the same experimental setup as in example 2 but with liquid solid ratio 10 4 acid concentrations of 1 and 5 were investigated at 80 u00b0 c and 100 u00b0 c sucrose hydrolysis results are presented in table 14 yields after finished hydrolysis are shown in table 2 the yields are expressed on a weight basis as the mass of produced monosaccharides per mass of hydrolyzed sucrose from the yields it can be seen that the best results are achieved by low acid loadings and high temperatures after one hour the yields were close to 100 and heating for another hour did not generate any significant sugar degradation a 12 l fermenter was used a media constituted by the substances given in table 3 was mixed and sterilized in the fermenter 0 5 l inoculum of a pure afp184 an e coli mutant described in u s published application 200300 7559 to donnelly et al grown for 16 hours in sterile tryptic soy broth was added together with a 2 l solution with a concentration of 400 g l of.