a clarified solution having a protein content of u2018 b u2019 g l the clarified protein solution or a u2018 c u2019 aliquot of the protein extract solution was reduced in volume to u2018 d u2019 l on a ultrafiltration system using a u2018 e u2019 dalton molecular weight cut off membrane the resulting concentrated protein solution had a protein content of u2018 f u2019 g l the lots were given designation u2018 g u2019 the parameter u2018 a u2019 to u2018 g u2019 are given in the following table iv table iv g bw al011 j16 01a bw al017 d11 02a a 24 4 26 3 b 20 3 18 0 c 1 2000 d 152 e 3000 5000 f 287 285 9 50 ml retentate aliquots of lot bw al011 j16 01a were warmed to 30 u00b0 c and 60 u00b0 c before being diluted 1 10 into 4 u00b0 c water in each case a white cloud of protein micelles formed immediately and was allowed to settle the upper diluting water was removed and the precipitated viscous sticky mass pmm was dried the pmm was recovered from each experiment and the yield of the dilution step was calculated in the case of the retentate temperature being 30 u00b0 c the protein recovery was 57 1 wt while for 60 u00b0 c the yield was 23 7 wt 5 ml retentate aliquots of lot bw al017 d11 02a were warmed to various temperatures between 30 u00b0 c and 60 u00b0 c and then diluted at dilution ratio of 1 10 or 1 15 into 4 u00b0 c water in each case a white cloud of protein micelles formed immediately and was allowed to settle the upper diluting water was removed and the precipitated viscous.