lysis buffer then incubated with template primer hybrid and digoxigeninlabeled and biotin labeled nucleotides the resulting dna has biotin and digoxigenin groups attached the biotin binds to a streptavidin bound plate surface and the digoxigenin is bound by an antidigoxigenin linked to peroxidase the complexes are washed and reacted with substrate to give a colored product values greater than or equal to 10 u03bcg well were scored positive for virus production hematologic data was collected and analyzed as outlined in example 1 results fig4 a 4b list hematologic data for the two ptx treated animals wherein the date refers to days after treatment with negative values showing pretreatment points these data include absolute cell counts but are otherwise the same as for previous data virus isolation and viral dna load determination was evaluated in the animals of example 3 virus isolation was performed on isolated pbmc approximately 7 months after the ptx treatment specifically 4 7 ml of whole heparinized blood was carefully layered over 5 ml of ficoll solution histopaque 1077 sigma chemical company st louis mo ficoll tubes were spun in refrigerated centrifuge beckman tj 6 at 800 g for 30 minutes plasma was carefully drawn off and the cells at the interface washed with phosphate buffered saline pbs after pbs wash the cells were resuspended and treated with 2 ml of ack buffer to remove unwanted red blood cells after ack treatment the cells were washed again in.