2 mm connected to a supelco supelguard lc si 5 u03bc guard column 20 u00d7 4 6 mm procyanidins were eluted by linear gradient under the following conditions time a b 0 82 14 30 67 6 28 4 60 46 50 65 10 86 70 10 86 followed by an 8 min re equilibration mobile phase composition was a dichloromethane b methanol and c acetic acid water at a volume ratio of 1 1 a flow rate of 0 5 ml min was used components were detected by fluorescence where u03bb ex 276 nm and u03bb em 316 nm or by uv at 280 nm a representative hplc chromatogram showing the separation of the various procyanidins is shown in fig2 b for one genotype similar hplc profiles were obtained from other cocoa genotypes 250 u00d7 3 2 mm phenomenex lichrosphere silica 100 column 5 u03bc 20 u00d7 4 6 mm supelco supelguard lc si 5 u03bc guard column procyanidins were purified by liquid chromatography on sephadex lh 20 28 u00d7 2 5 cm columns followed by semi preparative hplc using a 10 u03bc u03bcbondapak c18 100 u00d7 8 mm column or by semi preparative hplc using a 5 u03bc supelcosil lc si 250 u00d7 10 mm column partially purified isolates were analyzed by fast atom bombardment mass spectrometry fab ms on a vg zab t high resolution ms system using a liquid secondary ion mass spectrometry lsims technique in positive and negative ion modes a cesium ion gun was used as the ionizing source at 30 kv and a u201c magic bullet matrix u201d 1 1 dithiothreitol dithioerythritol was used as the proton donor analytical investigations of these fractions by lsims revealed the presence of a number.