bacteriostasis and or preservative effectiveness testing the present apparatus comprises an autoclavable collection bottle enclosing a collection chamber and an autoclavable closure adaptor means for closing the collection bottle while collecting a sample the apparatus has at least two passageways each in open communication at one end with the collection chamber a first passageway comprises a means for receiving and attaching the uncapped top portion of a sample container such that the liquid or gel contents of the container can be dispensed through the first passageway and into the collection chamber of the bottle a second passageway is adapted for communication with a vacuum source preferably via a flexible conduit or hose the closure adaptor means may be integrally molded from a suitable plastic material able to withstand the temperatures used during autoclaving in a preferred embodiment the closure adaptor means is an assembly comprising a plastic retainer ring internally threaded for attachment to the externally threaded neck portion of a transparent collection bottle the top of the retainer ring having a circular opening for securely holding a flanged substantially cylindrical stopper in which the above mentioned first and second passageways are formed as indicated above the first passageway provides a means of transferring the internal contents of a sample container that is securely and sealingly attached.