2 micron to remove bacteria 160 g of the secondary water together with oxygen gas was filled into a sealed aerosol container the aerosol container was made of aluminum and was lined with a resin coating on the inside the maximum pressure in the aerosol container was 8 kg cm 2 g gauge at 356 degrees c and the sealed container was confirmed to be safe the oxygen in the aerosol container has a partial pressure of 0 5 atm at 25 degrees c which ensures that the oxygen was dissolved in the bacteriostatic water at 20 ppm the bacteriostatic water filled in the aerosol container in the above step was stored at 35 degrees c for 2 weeks after b 2 weeks at 35 degrees c the bacteriostatic water was then tested for the number of bacteria the testing was performed as specified by the japanese pharmacopoeia to count the number of bacteria of general types and colon bacilli it was found that bacteria of general types were present at below 30 ml that is below the measurable limit and the product water tested negative for colon bacilli the same detection procedure was used for bacteriostatic treated water stored at various room temperatures ranging from 5 degrees c to 35 degrees c for a year and the same results as above were obtained 30 ml or less with respect to the genera bacteria and negative with respect to colon bacilli the following test was conduct to investigate the sterilizing and bacteriostatic effects.