10 5 cpm in 2 5 u00d7 10 5 pfu a or non radiolabeled hsv 1 kos b were pretreated with no drug white bar 7 u03bcm duy11 striped bar or 100 u03bcg ml heparin black bar for 5 minutes at 37 u00b0 c the samples were then cooled on ice inocula were diluted 1 20 1 100 in ice cold serum free media supplemented with no drug 7 u03bcm duy11 or 100 u03bcg ml heparin vero cells were infected with the so pretreated virions at 4 u00b0 c for 1 hour washed three times with ice cold serum free medium and lysed bound virions were quantified by liquid scintillation error bars represent ranges of three experiments a based on these results 7 u03bcm duy11 did abrogate infectivity fig1 b but did not inhibit virion binding fig1 a duy11 was next evaluated for its ability to affect high affinity binding fig1 is a line graph showing competition of hsv 1 binding to cells by increasing concentration of heparin 35 s methionine radiolabeled hsv 1 approximately 3 5 u00d7 10 5 cpm in 2 5 u00d7 10 5 pfu was pretreated with 7 u03bcm duy11 u25aa or with no drug ue8a0 for 5 minutes at 37 u00b0 c inocula were cooled on ice and then diluted 1 10 in ice cold serum free media with or without 7 u03bcm duy11 following 15 minute adsorption onto vero cells unbound virions were washed away bound virions were then further washed for 1 hour with media supplemented with 0 to 1 000 u03bcg ml heparin to remove those virions bound by only low affinity binding virions stripped away by these washes were then quantified by liquid scintillation error.