3 coco 2 00 s 3h ch 3 co nmr 1 h in agreement with the formula and the theoretical nmr spectra software acd labs demo nmr 13 c 200 mhz varian solvent cdcl 3 reference tms u03b4 in ppm 188 9 188 7 2c carbonyls 170 2 1c acetyl 157 8 157 9 2c pyruvyl 68 9 1c ch 61 5 59 4 2c ch 2 o 24 6 2c ch 3 18 4 1c ch 3 in agreement with the formula and the theoretical nm spectra software acd labs demo ir cm 1 ccl 4 3537 oh bonds from hydrated c u2550 o 2984 1756 1751 1740 1736 1729 c u2550 o 1383 1231 the nmr and ir spectra show that two molecules of water are fixed on carbonyl groups to form stable hydrated keto esters incubation of dpag with pig liver esterase liberates the components of the ester which were determined by enzymatic assays thus confirming the formula of dpag pdag was prepared in 81 yield by reacting diacetyl glycerol with pyruvyl chloride using the above procedure the formula of pdag was verified by i nmr 1 h and 13 c ii infrared spectra iii enzymatic assay of the components of dpag after hydrolysis and iv hplc before and after hydrolysis.