of 20 cc of sterile air inflation of the pouches was maintained by re injection of sterile air every other day until the pouches were ready to use on day 7 suspensions of cultured glioma cells total number 1 u00d7 10 7 cells were injected into the air pouches the air pouches were re inflated with sterile air every other day for the first seven days after inoculation with tumor cells by the seventh day sufficient tumor growth had occurred and sterile air injection was stopped by day 14 the tumors had reached sufficient size for experiments with the proposed treatment with an average tumor weight of about 70 gm and overall individual rat weight of about 250 gm plap with phospholipase a 2 activation activity is described in 34 tumour necrosis factor cachectin induces phospholipase a 2 activity and synthesis of a phospholipase a 2 activating protein in endothelial cells 34 by mike a clark mann jy chen stanley t crooke and john s bomalaski biochem j vol 250 pp 125 132 1988 synthetic plap peptides have been described in 34 cloning of a phospholipase a 2 activating protein 34 by mike a clark lynne e ozg ur theresa m conway janice dispoto stanley t crooke.