factors guifoyle t j 1997 genetic engineering 19 15 47 meisel and lam 1997 genetic engineering 19 183 199 promoters exemplified herein are set forth in seq id nos 1 4 in one embodiment of the invention to further delineate the sequences required for root expression as well as those regulatory sequences that influence the overall level of expression deletions of the root specific promoter regions are made deletions are made in the 5 u2032 flanking region of each root specific clone in most promoters 500 1000 base pairs bp of 5 u2032 flanking sequence are sufficient for promoter activity including tissue specific activity deletions of the 5 u2032 flanking region can result in promoter regions of approximately 50 bp 100 bp 250 bp 500 bp 750 by and 1000 by or more these promoter deletion sequences serve a two fold purpose the deletions allow the further mapping of regulatory sequences within the 5 u2032 flanking sequence of each root specific genomic clone additionally the deletions provide a toolbox of promoter and regulatory sequences that vary in their expression levels and expression patterns thus providing additional flexibility in choosing promoter sequences for appropriate gene regulation exemplified herein are fully functional shorter fragments of a promoter designated mrs1 seq id no 1 and a promoter designated mrs2 seq id no 2 the fully functional shorter fragment of mrs1 comprises nucleotides 603 1392 of seq id no 1 and is designated mrs1s one fully functional.