a set point temperature of 300 degrees farenheit roll actually heated to 325 340 during processing and at a pressure of about 1100 u00b1 100 psi the fabrics were subsequently face finished on the non calendered side fabric weight fabric weight was tested in accordance with astm designation d3776 96 air permeability was tested in accordance with astm designation d 3776 96 moisture vapor transmission mvtr was tested in accordance with astm designation e96 93 it is generally considered that variations in mvtr must be u00b1 25 g sq m 24 hrs to have effective significance in comfort differentiation an apparatus based on the design reported by vaughan j w et al jaci 1999 103 227 231 was used to test allergen barrier properties of the fabrics airflow measurements were calibrated against a fabric control with a known airflow rate five hundred milligrams of a dust sample with known amounts of the indicated allergens were pulled across each fabric a filter cassette mounted downstream from the fabric collected any allergen that was able to penetrate the fabric the filter was extracted in 2 0 ml of 1 bsa in pbs tween 20 overnight the extract was assayed the following day with an elisa for the relevant allergen the allergens measured were mite allergen der p1 and der f1 and cat allergen fel d1 in the tests the dust samples included 8982 ng of fel d1 and 1015 ng of der f1 respectively it is generally considered that fabrics having a fel d1 measurement of lt 0 31 would be an effective barrier to cat dander allergen transfer smaller particle size.