by a single copy two exon gene near the telomeric region of chromosome 5 nucleotide sequences 64605 64133 and 64332 65489 it does not have discernible orthologues in other organisms blastp cut off e value of 10 u2212 15 both pfsa1 and pfsa2 are highly conserved in multiple strains of p falciparum from various geographic locations fig6 suggesting their potential utility in vaccine construction rabbit antisera were raised against synthetic peptides designed based on pfsa1 and pfsa2 the peptide sequence used for pfsa1 is nnskfskdgdnedfnnkndlynpsdklynn seq id no 5 the peptide sequence used for pfsa2 is yeimhkedeskesnqhnykegpsyedkknmyke seq id no 6 two specific antibodies denoted 108 and 112 recognized proteins corresponding to the theoretical mw of pfsa1 and pfsa2 respectively in the whole cell lysate and the biotin labeled fraction fig3 to confirm the surface location of the pfsa1 and pfsa2 we labeled the intact prbc in suspension with purified igg from antisera 108 and 112 followed by incubation with goat anti rabbit and chicken anti goat alexa fluor 488 as secondary and tertiary antibodies ethidium bromide was added to the incubation to stain the nuclei the cells were allowed to adhere to cover slips pre coated with polylysine and.