at 35 55 u00b0 c preferably 40 45 u00b0 c for 5 minutes the milled bone powder is treated with hcl cacl 2 and edta at 4 u00b0 c as described in detail below and the entire procedure takes approximately 48 hours no chloroform or methanol extraction process is used and no lithium chloride solution is used in the process for obtaining the novel isbg of this particular embodiment the isbg produced is useful in preparing impaction bone grafts in one embodiment the present invention includes the steps of screening and testing human bones to determine suitability for human transplantation preparing bone powder preferably having a particle size up to 1 0 millimeter more preferably 0 5 1 0 millimeter isolating and purifying insoluble bone gelatin from the bone powder and using the purified insoluble bone gelatin for example for impaction bone grafting non union fracture and dental use in one embodiment of the isolation and purification procedure according to the present invention bone powder prepared according to the present invention was demineralized using an acid such as hydrochloric acid or acetic acid then treated with a neutralizing salt such as calcium chloride or calcium phosphate and then treated with a stabilizer such as ethylene diamine tetraacetic acid edta the.