the words u201c comprise u201d and u201c contain u201d and variations of these words mean u201c including but not limited to u201d and they are not intended to and do not exclude other moieties additives components integers or steps throughout the description and claims of this specification the singular encompasses the plural unless the context otherwise requires in particular where the indefinite article is used the specification is to be understood as contemplating plurality as well as singularity unless the context requires otherwise disclosed herein are devices and methods of growing cells tissues and organs for use in preparation of surgical grafts including autografts and allografts an autograft is a graft prepared from the recipient 39 s own tissue for example from a healthy organ of the recipient an allograft is a graft of tissue between individuals who are not genetically identical an allograft may be prepared from tissue obtained from a cadaveric organ or living related individual for example the invention also relates to a method of expanding cells for example mesenchymal cells such as mesenchymal stem cells which can be activated and mobilised if needed mesenchymal stem cells are adult stem cells that can form a variety of cells including fat cells cartilage bone tendon and ligaments muscles cells skin cells and nerve cells for example expanded mesenchymal stem cells could be used to re generate endocrine tissue for example isles of langerhans cells for implanting into a patient suffering from diabetes in another example although damage to muscles heals very slowly if mesenchymal stem cells expanded in the disclosed system were activated and implanted in the damaged muscle tissue then such wounds would very likely heal much faster non limiting examples of three possible design variations of the disclosed.