nucleic acids to monocot species in the generation of transgenic plants for use in methods according to the invention smith et al crop science 2 301 309 1995 incorporated herein by reference other transformation methodologies may also be employed to generate transgenic plants as would be understood in the art for example direct dna transfer into plant cell protoplasts may be effected by the conventional techniques of calcium phosphate co precipitation the use of poly l ornithine liposome mediated transformation electroporation microinjection or fusagen mediated e g polyethylene glycol transformation and plants regenerated from the transformed protoplasts pct us84 00050 and christou euphytica 85 13 27 1995 each incorporated herein by reference other transfer methodologies such as biolistic transformation i e microprojectile or particle bombardment do not require plant cell protoplasts thereby simplifying the process of regenerating transgenic plants consequently biolistic transformation may be employed to introduce the coding region for a heterologous polypeptide into a wide variety of plants including both monocots and dicots christou 1995 jahne et al euphytica 85 35 44 1995 incorporated herein by reference regeneration of transgenic plants from transformed cells including transformed protoplasts may be accomplished using any one of several techniques known in the art several approaches to the regeneration of transgenic plants are disclosed in ep a 0 243 553 incorporated herein by reference these approaches include regeneration via embryogenic or organogenic routes alternatively plants may be regenerated following transformation by a method that incorporates a step for inducing meristem reorganization to improve.