was then added to all of the wells to stop the reaction the plate was then run through an elisa reader the results of this assay are provided in table 1 below these results indicate that when the incubation time is extended expression of orf2 into the supernatant of the centrifuged cells and media is greater than expression in the pellet of the centrifuged cells and media accordingly allowing the orf2 expression to proceed for at least 5 days and recovering it in the supernate rather than allowing expression to proceed for less than 5 days and recovering orf2 from the cells provides a great increase in orf2 yields and a significant improvement over prior methods this example provides data as to the efficacy of the invention claimed herein a 1000 ml spinner flask was seeded with approximately 1 0 u00d7 10 6 sf cells ml in 300 ml of excell 420 media the flask was then incubated at 27 u00b0 c and agitated at 100 rpm subsequently the flask was seeded with 10 ml of pcv2 orf2 bac p 6 the recombinant baculovirus containing the pcv2 orf2 gene passaged 6 additional times in the sf9 insect cells virus seed with a 0 1 moi after 24 hours of incubation the flask was then incubated at 27 u00b0 c for a total of 6 days after incubation the flask was then centrifuged and three samples of the resulting supernatant were harvested and inactivated the supernatant was inactivated by bringing its temperature to 37 u00b1.