after isolation in basal nutrient media media 199 containing 5 fetal bovine serum which may contain 10 ng ml platelet derived growth factor bb different types of substrate on tissue culture plates can be used mesothelial cells of this invention may be cultured in serum free nutrient media or serum containing nutrient media as is well known to those of ordinary skill in the art serum is commonly added to nutrient media to further enhance cell growth serum contains many serum biomolecules however the mesothelial cells of this invention may be grown in the absence of a plurality of these serum biomolecules cell growth of mesothelial cells may be enhanced by the addition of one or more proteins found in serum for example but not limited to bovine serum albumin or bsa platelet derived growth factor epidermal growth factor fibroblast growth factor insulin like growth factor 1 insulin hepatocyte growth factor and transforming growth factors the frequency of feeding mesothelial cells may be once a day or every other day in one embodiment mesothelial cells may be fed by replacing the entirety of the old nutrient media with new nutrient media in another embodiment mesothelial cells may be fed with conditioned media in which these cells were grown because the claimed mesothelial cells are unique to this invention and will secrete factors specific to these cells the conditioned media derived from.