the gel 62 with the tissue particles 71 to create a mixture 80 within the container 170 and thereafter to aspirate the mixture 80 from the container 170 in operation as shown in fig5 and 7 a 7 f the surgical blade 10 is brought into contact with a desired bodily tissue such as synovial or adipose tissue fig5 the operator cuts a desired amount of tissue from the donor site using the blade 10 the vacuum source 90 aspirates fluid and the cut tissue through the aspiration lumen 16 of the inner tubular member 14 to the tissue collection device 140 during aspiration the fluid and cut tissue flow through the filter 147 which removes undesirable cut tissue from the fluid pathway such as particles larger than for example about 0 6 mm to about 2 4 mm after passing through the filter 147 the remainder of the fluid and cut tissue pass through the filter 148 which removes undesirable cut tissue from the fluid pathway such as particles larger than for example about 0 6 mm to about 1 mm the remainder of the fluid and cut tissue pass through the filter 149 where tissue particles 71 fig7 b of a desired size such as particles larger than for example about 0 5 mm to about 50 u03bcm are isolated and or retained on the filter 149 the remainder of the cut tissue and fluid volume pass through the tissue collection device 140 and are aspirated to the collection apparatus not.