continuous exponential feeding strategy resulted in maintaining cells in stationary phase for a longer duration thereby increasing yield of n meningitidis x polysaccharide use of casamino acid as a nitrogen source was found to provide high yield amp high molecular weight men x polysaccharide with minimal impurities at 100 kda stage as compared to soya peptone as nitrogen source when only dextrose and sodium glutamate were used as feed solution fs 2 cell morphology was affected ultimately resulting in unfavorable polysaccharide characteristics whereas when fs 1 containing amino acids salts in addition to dextrose and sodium glutamate was used morphology of cells was found to be improved thereby increasing polysaccharide yield and providing polysaccharide with favorable characteristics study of growth profile of n meningitidis x 8210 reveals that during fermentation a ph drop resulting from accumulation of released cellular metabolites was observed that was adversely affecting rate of capsular polysaccharide production to avoid such ph drop naoh at a concentration between 0 25n and 0 5n and orthophosphoric acid at a concentration between 5 and 10 was continuously provided by the dosing pumps in cascade with the ph kept at set point wherein ratio of sodium carbonate 20 to naoh 0 5n is maintained at 1 3 for nmxm ca medium containing casamino acids at 100 kda stage polysaccharide yield was between 500 and 650 mg 1 with minimal load of wherein harvesting was carried at when od reached 50 of highest culture od whereas for nmxm sp medium containing soya peptone at 100 kda stage polysaccharide yield was comparatively low i e between 450 and 500 mg 1 with greater load of impurities the fermentation was terminated once drop in optical density was observed followed by inactivation using 1 formaldehyde for 2 hrs at 37 u00b0.