p2 5 u2032 ctaactaactaactacatacctcatcatatcc 3 u2032 seq id no 24 which is complementary to the 3 u2032 end of the first exon and to the 5 u2032 end of the intron and introduces a stop codon underlined three constructs lacking the sequence encoding the transit peptide were also produced with the oligonucleotide p3 5 u2032 cctctctttccatatgctcc cttctc 3 u2032 seq id no 25 which introduces an nde i restriction site underlined at the second atg codon m43 at position 127 the oligonucleotide p4 5 u2032 ccgccagccacct ccatatgaccgacaccatcc 3 u2032 seq id no 26 which introduces an atg initiating codon and an nde i restriction site underlined at position 174 from the first atg codon v58m and the oligonucleotide p5 5 u2032 cgccacccctcatatgcgtatcgcc 3 u2032 seq id no 27 which introduces an atg initiating codon and an nde i restriction site underlined at position 222 from the first atg codon l75m all the ocr fragments corresponding to the first exon which may or may not encode a transit peptide were cloned into the plasmid ppcr script stratagene nde i bamh i dna fragments containing the coding sequences with or without the transit peptide sequence were then cloned into the plasmid pet21 a.