same substrate to make the same product as method i r fascians atcc 12975 culture was grown in a 380 liter fermentor containing 250 liters of medium 1 growth consisted of inoculum development and fermentation stages inoculum development consisted of f1 and f2 stages in the f1 stage frozen vials of r fascians atcc 12975 culture were inoculated into 100 ml of medium 2 containing 2 glucose 1 yeast extracts 1 malt extracts and 0 1 peptone growth was carried out in 500 ml flasks at 28 u00b0 c 280 rpm for 48 hours in the f2 stage 100 ml of f1 stage culture were inoculated into 1 5 liters of medium 2 in a 4 liter flask and incubated at 28 u00b0 c 180 rpm for 24 hours a germinator containing 250 liters of medium a was inoculated with 1 5 l of f2 stage inoculum and grown for 24 hours at 28 u00b0 c 150 rpm 200 lpm liter per minute aeration fermentors containing 250 liters of medium 1 were inoculated with 13 liters of germinator growth inoculum fermentations were conducted for 40 hours at 28 u00b0 c 250 rpm and 200 lpm aeration and ph 6 8 7 0 compound xa 5 grams in 30 ml of dimethylformamide was added as an inducer after 22 hours of growth in a fermentor to determine the specific activity of cells during fermentation cells were periodically harvested by centrifugation from 200 ml of culture broth cell suspensions 10 w v wet cells were prepared in 0 1 m phosphate buffer ph 6 8 and 250 u03bcgm ml of compound xa in dimethylformamide was added the biotransformation was conducted in a 125 ml flask with a reaction volume of 25 ml at 37 u00b0 c 280 rpm for 2 hours periodically.