created by combining the maximal intensity profiles of each z stack image see fig2 5a because the maximal intensity of each section corresponds to in focus platelets the light intensity of the composite 3d image correlates with the overall shape of the observed aggregate with white representing the maximum height and black the minimum light intensity plots and 3d profiles of platelet aggregates were created using imagej plug in interactive 3d surface plot v2 33 see fig2 5 b d barthel 2011 a plateau was observed when the maximum intensity white was reached normalized and averaged light intensity profiles of nine aggregates show an asymmetric aggregate geometry aligned in the direction of the flow image analysis shows an abrupt increase in light intensity upstream of the aggregate linear increase with a slope of 9 6 with a more gradual decrease downstream linear decrease with a slope of 5 2 see fig2 6 the apex or maximal height of each aggregate was typically located at the proximal end of each aggregate upstream side the capacity of the flow chamber to support extended duration perfusion culture of endothelial cells was tested specifically we used the device to assess the potential of the decellularized huv to support an endothelial cell monolayer under physiological flow conditions primary endothelial cells were seeded onto.