plasmid pso124 u03b4c bacillus subtilis db104 transformed with this plasmid is designated and indicated as bacillus subtilis db104 pso124 u03b4c and deposited on may 16 1997 the date of the original deposit under budapest treaty at the national institute of bioscience and human technology agency of industrial science and technology ministry of international trade and industry 1 3 higashi 1 chome tsukuba shi ibaraki ken japan under accession number ferm bp 6294 the expression level of protease of this transformant is increased as compared with that of bacillus subtilis db104 pnaps1 the enzymological properties as well as the physical and chemical properties of the proteases produced by the transformants bacillus subtilis db104 pnaps u03b4c and bacillus subtilis db104 pspo124 u03b4c appear to be identical with those of the protease produced by bacillus subtilis db104 psnp1 the main properties of the proteases obtained from the cultures of the two transformants are as follows exhibits an enzymatic activity at 40 110 u00b0 c with the optimal temperature being 80 95 u00b0 c exhibits an enzymatic activity at ph 5 10 with the optimal ph being ph 6 8 retains 90 or more of its enzymatic activity after treatment at 95 u00b0 c for 8 hours retains 95 or more of its activity after treatment at ph 5 11 at 95 u00b0 c for 60 minutes thus proteases having.