nisshinbo and sold by aroka ltd co was used as a sequencing primer and a sequencing kit trade name sequitherm u2122 long read u2122 cycle sequencing kit lc manufactured by epicentre technologies company was used as reaction solution the base sequence was determined by a laser system for reading dna sequences trade name 4000l long readir u2122 dna sequencing system manufactured by li cor company the resulting 16s ribosomal rna gene sequence of the pectinatus sp dsm20764 is shown in sequence no 21 oligonucleotides which had the same sequence as that of no 630 to no 647 on the sequence of the 16s ribosomal rna gene of the pectinatus sp dsm20764 in sequence no 21 and oligonucleotide which had a complementary sequence to that of no 1004 to no 1022 were chemically synthesized the pcr of dna solution of each bacterium obtained by preparation of samples was conducted by using the synthesized oligonucleotides the pcr was repeated for 35 cycles and one cycle had the following temperature conditions after the conclusion of the pcr the solution was electrophoresed by agarose gel at 100v constant voltage for 30 minutes at the same time o slashed x174 hincii was electrophoresed as a molecular weight marker after the electrophoresis the gel was dyed in a ethidium bromide solution about 0 5 u03bcg ml for 15 minutes the gel was observed under ultraviolet irradiation and photographed by the observation.