mm dish thereby high titer of viruses was obtained then precipitates of viral particles were produced by centrifugation after adding 2 ml of 50 peg6000 to the cell culture medium next proteinase k was added to the mixture at 42 u00b0 c and left alone for 2 hours to dismantle the viral envelope the viral lysate was extracted twice using the same amount of phenol chloroform and ethanol was added thereto thereby precipitated and purified vbachts2 viral dna seq id no 8 the sequence of its polyhedrin locus were obtained then cell lines life technologies inc maryland u s a were transformed with the purified dna using micropulser bio rad california u s a which are in turn incubated in cm medium to form a bacterial colony the bacteria were incubated in 1 liter of 2xyt medium 10 g of yeast extract powder 16 g of tryptone 5 g of nacl within per liter then dna was purified and named as bbachts2 fig1 thus according to this method it is possible to reduce the required time period and cost significantly since the viral dna can be obtained from bacterial culture without the need of incubation in insect cells purified 10 u03bcg of dna was digested with 50 units of bsu36i at 37 u00b0 c for 5 hours which in turn was heat treated at 80 u00b0 c for 20 minutes to inactivate bsu361 enzyme likewise vbachts2_gfp seq id no 9 was prepared from pbachts2_gfp vector by homologous recombination and then was transferred to dh10b by electrophoration the dna was purified and named as bbachts2_gfp see.