babesiosis microti cryptosporidiosis cryptosporidium parvum dientamoebiasis dientamoeba fragilis giardiasis giardia lamblia helminthiasis and trichomonas trichomonas vaginalis the above lists are meant to be illustrative and by no means are meant to limit the invention to those particular bacterial viral or parasitic organisms in another embodiment the pathophysiological insult can be due to the development and progression of a disease state e g associated with cell death programmed cell death cell damage necrosis etc in one embodiment the pathophysiological insult is tumorigenesis or neoplasia in a subject in another embodiment the pathophysiological insult is substantial tumorigenesis or neoplasia at more than one location in a subject for example metastasis in lymph nodes etc examples of diseases resulting from tumorigenesis or neoplasia include but not limited to leukemia breast cancer prostate cancer liver cancer stomach cancer colon cancer melanoma lymphoma lung cancer pancreatic cancer brain tumor oral cancer etc in yet another embodiment the pathophysiological insult is an autoimmune disease examples of autoimmune disease include vertiligo scleroderma rheumatoid arthritis chagas disease diabetes mellitus type 1 hashimoto disease ankylosing spondylitis grave 39 s disease guillain barre syndrome etc in yet another embodiment the pathophysiological insult is a cardiovascular disease examples of cardiovascular disease include acute vascular obstruction such as pulmonary embolism and cardiac infarction resulting in apoptosis of heart cells which in turn causes the release of cell free nucleotides e g dna into circulation in yet another embodiment the pathophysiological insult is hepatic disease lung disease or kidney disease etc especially conditions associated with cell death and necrosis in still another embodiment the pathophysiological insult is a traumatic physical.