the method of the present invention by substituting the reaction buffer recommended for the specific thermostable polymerase being used provided that an adequate number of units of enzyme are added to the reaction mixture the present inventors have successfully carried out the claimed method using the thermosequenase supplied by amersham taq polymerase supplied by boehringer mannheim biochemicals the circum vent thermostable polymerase of new england biolabs and the taq polymerase supplied by promega in the fmol kit the sequencing reaction is carried out in four 0 2 ml pcr tubes which are labeled a t g and c 2 0 u03bcl of the appropriate chain termination reaction mixture such as the long read u2122 lc termination mix from epicentre technologies is added to the appropriate tube the termination mix contains appropriate amounts of dntps and the corresponding dideoxynucleotides four u03bcl of the labeling reaction mixture from step 1 above is added to each termination tube one of skill in the art will recognize that the concentrations should be adjusted to the optimum for the particular thermostable polymerase used the contents of the tubes are mixed well then overlaid with a drop of mineral oil the tubes are carefully transferred to a thermal cycler and subjected to cycling temperatures of a cycle sequencing program which is optimized for the thermal cycler and primers being used an optimized program for the perkin elmer geneamp pcr cycler is a 92 u00b0 c for 2 minutes 92 u00b0 c for 30 seconds c 50 u00b0 c for 15 seconds and d 70 u00b0 c for 15 seconds steps b to d are repeated for a total of 30 cycles then the block is cooled to 4 u00b0 c optimized programs.