incubator for 5 days the plate was then emptied by inverting and blotted dry room temperature dpbs containing mgcl 2 and cacl 2 was dispensed into each well from a multi channel squeeze bottle tapped three times emptied by inversion and then blotted dry 50 microliters of the fluorescent calcein dye diluted in dpbs containing mgcl 2 and cacl 2 was added to each well and incubated at 37 u00b0 c in a 5 percent co 2 incubator for 30 minutes the plates were read in a perkin elmer hts7000 fluorescence plate reader and the data was analyzed in microsoft excel and the results were tabulated in table 2 the data represented an average of four experiments tested in triplicate and presented qualitatively in the following fashion 4 4 experiments greater than threshold cytotoxicity 3 4 experiments greater than threshold cytotoxicity 2 4 experiments greater than threshold cytotoxicity unmarked cells in table 1 represent inconsistent or effects less than the threshold cytotoxicity the chemical cytotoxic agents induced their expected cytotoxicity while a number of other antibodies which were included for comparison also performed as expected given the limitations of biological cell assays the ar2a72 10 antibody demonstrated cytotoxicity in multiple ovarian cancer cell lines and was selective in its activity since not all cancer cell types were susceptible to antibody mediated cytotoxicity furthermore ar2a72 10 demonstrated functional specificity since it did not produce cytotoxicity against non cancer cell types which is important for the therapeutic utility of the antibody binding of ar2a72 10 to the above mentioned panel of cancer and normal cell lines.