flouorescence detection amperometric detection conductometric detection and radiometric detection galactomannans contain relatively high content of proteins some of the proteins are basic with pi above 9 at the ph of capillary electrophoresis of dna when galactomannans can be potentially used as a sieving matrix those proteins behave as cations that interact with dna by strong coulombic interactions these interactions lead to a peak broadening and loss of resolution because of that the protein content has to be minimized in galactomannans that are to be used as sieving matrix generally speaking we apply three or four steps to remove proteins from the galactomannan preparations i hydrolysis with naoh if it is desirable to reduce the molecular weight of the galactomannan ii treatment with cation exchanger iii treatment with anion exchanger iv precipitation of galactomannan with acetone which can be repeated if desired precipitation in acetone both concentrates the guaran from dilute solutions and fractionates because the larger molecules are precipitated the specific purification methods 1 3 shown in examples 1 3 below combine these individual steps dna analysis can be performed using the preferred capillary column having an interior cavity filled with a composition made of an aqueous solution of 15 g l guaran 7m urea 50 mm tris and 50 mm hepes by introducing an aliquot of a sample into the capillary column applying an electric field of about 150 v cm to the capillary column separating the sample components and detecting them protein analysis can be performed using.