the rates of time dependent turbidity change in the presence and absence of inhibitor respectively thus an inhibition index greater than 1 indicates that the charged species had an inhibitory effect on aggregate formation a value of 1 implies no effect while less than 1 indicates an enhancing effect on the aggregation process measurements were performed 3 5 times and standard derivations are reported initially conditions were established over which 8 representative proteins could be induced to aggregate over a one hour time period the resultant turbidity measured by the o d at 360 nm versus time curves were characterized by distinct delay times before aggregation could be detected as well as by rates and extent of aggregation to detect any effects of potential inhibitors of the various parameters of the aggregation process an aggregation index that was proportional to the delay time but inversely related to the rate and extent of aggregation was defined thus values greater than one are evidence of inhibitor activity while values of less than one are evidence of enhanced aggregation two types of libraries were selected for screening by this assay the libraries are listed below in table 1 in the first library charged moieties amino and carboxyl groups were sequentially spaced by methylene groups thus a series of dianions dications and non.