hours the catalyst was removed by filtration through a celite bed and the ch 3 oh stripped the crude oil 0 50 g was taken up in h 2 o and applied to an ag50wx8 h 10 ml column eluting first with h 2 o then with 10 pyridine h 2 o the desired fractions were combined the solvent stripped and the residual pyridine azeotropically removed with toluene the residue was taken up in h 2 o filtered millipore and lyophilized to give a glassy solid 350 mg the solid was passed through an ag50wx8 li 10 ml column eluting with h 2 o the desired fractions were combined taken to small volume and chromatographed on an hp 20 200 ml column eluting with a linear gradient h 2 o ch 3 cn 0 u2192 90 the desired fractions were combined stripped to dryness taken into h 2 o filtered millipore and lyophilized to give the title product 90 mg 0 28 mmole 15 yield as a glassy solid tlc 7 2 1 isopropanol conc nh 4 oh h 2 o single spot r f 0 3 analysis calcd for c 13 h 23 n 2 o 5 pli 1 3 moles h 2 o c 43 91 h 7 25 n 7 88 p 8 7 found c 43 93 h 7 25 n 7 88 p.