to a comparable level to that of the wild type mv h protein moreover the blot shows that the displayed domains are not spontaneously cleaved from the chimeric mv h glycoproteins mv h expression constructs pcg h pcg h egf r pcg h xegf r pcg h igf and pcg h xigf were co transfected into the u03b2 galactosidase expressing c170 and a431 cells along with the mv f expression construct pcg f the cells were stained with x gal substrate 72 hrs after transfection to allow ease of cell cell fusion detection results of the assays are shown in tables 1 and 2 and in fig5 the chimeric mv h proteins were potent inducers of cell cell fusion in c170 cells although their potency was slightly reduced compared to the unmodified h protein table 1 fig5 cell cell fusion in a431 was abolished for the chimeric h proteins compared to the unmodified mv h protein which was a potent inducer of cell cell fusion table 2 1 foreign polypeptides can be displayed as fusions to the extreme c terminus of the mv h protein 2 the chimeric h glycoproteins are efficiently expressed and are functional in cell cell fusion assays 3 the displayed ligand can target the specificity of cell cell fusion the expression constructs of measles virus mv f and mv h protein were encoded by the expression plasmids pcg f and pcg h respectively catomen et al virology 214 p628 1995 fbdelpgasaf encodes the wildtype galv envelope and fbdelpgasaf fus encodes a c terminally truncated galv envelope lacking the cytoplasmic tail see attached sequence fig6 human c170 durrant.