supra e coli strain hb101 was transformed by plasmid pbr322 which has been subcloned with fragments of the human tsh u03b2 gene using similar mechanisms e coli and other prokaryotes may be so transformed additionally the state of the art is such that eukaryotic cells may be transformed by appropriate vectors such as viruses containing the human tsh u03b2 gene this allows for production of this protein in glycosylated form in vitro by amplification means known to the art it is also possible to increase the production of the protein to high levels perhaps the most interesting use of the isolated gene is in diagnostics various endocrine disorders are characterized by overproduction or underproduction of hormones including thyroid stimulating hormone one could administer htsh made by recombinant dna technology to humans in order to determine whether thyroid gland failure is due to primary thyroid disease or central pituitary or hypothalamic disease the terms and expressions which have been employed are used as terms of description and not of limitation and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof it being recognized that various modifications are possible within the scope of the invention tgatttagc atg act gct ctc ttt ctg atg tcc atg ctt ttt ggc ctt gca 111 tgt ggg caa gcg atg tct ttt tgt att cca act gag tat aca atg cac 159 cys gly gln ala met ser phe cys ile pro thr glu tyr thr met his atc gaa agg aga gag tgt gct tat tgc cta acc atc aac acc acc atc 207 tgt gct gga tat tgt atg.