positive cd8 cd15 population would also be positive for cd4 as in kwt tri hybrid derived from cd4 effector t cells the cd19 cells represented 52 of the total cell population with 22 of them co expressing cd15 thus the entire cd15 cell population co expressed cd4 cd8 and cd19 meaning 22 23 a kwt cross lineage tri hybrid cell line established from one immortal myeloid cell k562 immortal lymphoid cell wil2ns and cd5 positive antigen experienced t cell when cd5 positive antigen experienced t cells were used to generate a kwt cross lineage tri hybrid line the expression of cd5 was analysed the kwt hybrid cells were labelled with mouse anti human cd19 htc and mouse anti human cd5 pe antibodies using the same protocol as used for analysis of wtm cross lineage tri hybrids except for reversion of fluorescent conjugates between mouse anti human cd19 and mouse anti human cd5 antibodies section 4 6 the results shown in fig2 showed that 90 of the kwt cross lineage tri hybrid cells were positive for cd5 originating from antigen experienced t cell and 49 of the cd5 cells were also positive for cd19 originating from wil2ns cells b cells the total percentage of cd19 cells among the cross lineage tri hybrid cells was around 56 4 5 cross lineage tri hybrid production from two immortal lymphoid cells wil2ns and one primary monocyte u2014 wwm this type of cross lineage tri hybrid production was created by somatic cell hybridisation.