am j physiol 271 c1494 c1503 densmore j j szabo g and gray l s 1992 a voltage gated calcium channel is linked to the antigen receptor in jurkat t lymphocytes febs lett 312 161 164 haverstick d m and gray l s increased intracellular ca2 induces ca2 influx in human t lymphocytes molecular biology of the cell 4 173 184 1993 haverstick d m densmore j j and gray l s 1998 calmodulin regulation of ca2 entry in jurkat t cells cell calcium 23 361 368 it has been shown recently that a prostate cancer line expresses the u03b11h isoform of t type ca2 channels at levels that vary with differentiation status mariot p vanoverberghe k lalevee n rossier m f and prevarskaya n 2002 overexpression of an alpha 1h cav3 2 t type calcium channel during neuroendocrine differentiation of human prostate cancer cells j biol chem 277 10824 10833 using the same primers we identified two different amplicons in cancer cell lines fig3 the 170 base amplicon found in the malignant t cell line jurkat is similar to t type ca2 channel isoform u03b11h unigene cluster hs 122359 with an expectation value of 3e 79 in both forward and reverse directions for jurkat cells for du145 the expectation value in the forward direction was 1e 67 and in the reverse was 1e 70 the 320 base amplicon from the neuroblastoma cell line sk n sh is similar to the 825 splice variant genbank accession number af223563 with an expectation value of 1e 141 in the reverse direction and 1e 135 in the forward direction also shown in fig3 is the result of the rt pcr assay using message obtained from the hl60 human leukemia cell line the lack of a detectable pcr product is concordant with.