bovine eyeballs 1 2 eye physiological saline was added to the retinae to prepare bovine retinal homogenates 2 ferrous chloride 0 5 mm dissolved in distilled water was added to each of the retinal homogenates obtained in the above further 2 o octadecylascorbic acid and u03b1 tocopherol were added to the resulting homogenates to final concentrations of the reaction mixtures of 10 4 m respectively further distilled water was added to each of the homogenates to adjust a total volume to 1 ml 2 o octadecylascorbic acid and u03b1 tocopherol were dissolved and diluted with ethanol thus the final concentrations of ethanol in all of the reaction mixtures were adjusted to 1 then the mixtures were subjected to reaction at 37 u00b0 c for 1 hour 3 0 35 thiobarbituric acid hereinafter referred to as tba reagent 50 aqueous acetic acid solution 1 ml was added to the reaction mixtures or standard solutions of the retinal homogenates obtained in the above 2 the mixtures were subjected to reaction in boiling water at 100 u00b0 c for 1 hour after water cooling n butanol 2 ml was added and the mixtures were shaken for 5 minutes and centrifuged at 3000 rpm for 10 minutes fluorescence intensities excitation wave length 515 nm emission wave length 553 nm were determined for the n butanol layer proteins were determined using bio rad protein assay kit trade name table 1______________________________________ retina tba value inhibitory nm malondialdehyde ratio mg protein ______________________________________blank 1 ethanol 0 34 u00b1 0 01 2 100ferrous chloride 5 65 u00b1 0 13 3 0 control 2 o octadecyl 10 sup 4 m 0 61 u00b1 0 04 3 94 9ascorbic acid u03b1 tocopherol 10 sup 4 m 2 38 u00b1.