fibre formation fibrillogenesis was performed as described above cs e from squid was purchased from calbiochem 0 13 u03bcg ml corresponding to the molar concentration of gag chains in previous experiments using recombinant perlecan domain i variants with hs cs pg ib 33 nm the results of the fibrillogenesis experiment is shown in fig2 as can be seen from this figure cs e but none of the other tested chondroitin sulphate variants cs d and cs 6 gives a positive result on collagen fibril formation cff fig2 cs e accelerated collagen fibril formation different types of purified cs chains were tested for effect in the collagen fibrillogenesis assay panel a the highly sulphated cs e had dramatic effects on fibril formation but neither cs 6 nor cs d had any effect the stimulatory effect of cs e was dose dependent reaching saturation at concentration of 30 u03bcg ml panel b groups of 5 icr male mice weighing 22 u00b1 2 gms are used under hexobarbital 90 mg kg i p anesthesia the shoulder and back region of each animal is shaved a sharp punch id 12 mm is used to remove the skin including panniculus carnosus and adherent tissues the wound area traced onto clear plastic sheets on days 3 5 7 9 and 11 are quantitated by use of an image analyzer life science resources vista version 3 0 test compound.