j 5 hz 4 99 1h t nch 2 c h 2 f j 5 hz 6 41 1h s c h c ch 3 ch 6 61 1h s chc ch 3 c h 6 99 7 05 2h m 2 ar 4 c h 7 08 1h m 2 ar 6 c h j 5 hz 7 23 1h m 2 ar 6 c h j 5 hz 7 74 2h m 2 ar 7 c h j 5 hz 35 mg was isolated from the same reaction set forth in example 5 lc ms m z calcd for c 24 h 26 fn 2 o 3 s 2 475 59 and found 475 6 m 1 h nmr 300 mhz dmso d6 2 58 3h s chc c h 3 ch 3 91 6h s oc h 3 4 85 4h bs nc h 2 c h 2 f 4 93 2h bs nch 2 c h 2 f 5 00 2h bs nc h 2 ch 2 f 6 58 2h s c h c ch 3 c h 7 09 2h dd 2 ar 6 c h j 3 9 hz 7 4 2h bs 2 ar 6 c h 7 95 2h d 2 ar 7 c h j 9 hz tau protein tau 412 with 2 nucleotide binding motifs and 4 repeat binding regions 42 9 kda 1 mg ml stratech was incubated in the presence of heparin 0 1 mg ml for 72 h at 37 u00b0 c with continuous shaking to generate tau aggregates a u03b2 1 40 protein 4 3 kda 1 mg ml invitrogen was incubated at.