invention the isolation of cellulose producer s was carried out using six different fruit residues apple pineapple orange sweet lime pomegranate and mixed here each fruit residue was mixed with sugar and water in the ratio of 1 0 2 3 respectively the mixture was kept in a wide mouthed plastic container and covered with a piece of cloth the container was kept at temperature of 30 degree c for 10 days undisturbed and observed for mc production after 10 days it was observed that at the top of the pineapple orange sweetlime and mixed fruit residue mixtures a mat like structure was deposited this mat like structure was analyzed for the presence of cellulose fibrils by calcofluor staining and electron microscopy the results showed that the mat like structure was composed of a network of ultrafine cellulose fibrils and it also contained rod shaped bacterial cells producing cellulose fig1 3 amp 4 screening of the bacterial isolates obtained from fruit residues for microbial cellulose production all the isolates obtained from different fruit residues were evaluated for their potential to produce microbial cellulose these isolates were inoculated individually in 250 ml erlenmeyer flasks containing 50 ml cellulose production medium hestrin schramm medium containing g l glucose 20 peptone 5 yeast extract 5 disodium hydrogen phosphate 2 7 and citric acid 1 15 and incubated for 15 days at 30 degree c under static conditions for cellulose production a compact mat was formed.