and examined by light microscopy scid mice were injected subcutaneously in the dorsolateral flank with 5 u00d7 10 6 h460 cells the mice bearing size matched lung cancer xenografts approximately 300 mm 3 were intravenously injected with 2 u00d7 10 9 pfu of the targeting phage or control phage after eight minutes of phage circulation the mice were sacrificed and perfused with 50 ml pbs to wash out unbound phage subsequently xenograft tumors and mouse organs were dissected and homogenized the phages bound to each tissue sample were recovered through the addition of er2738 bacteria and titered on iptg x gal agar plates for the in vivo whole body imaging hpc1 2 4 and control phages were labeled with the fluorescence dye hilyte u2122 fluor 750 acid nhs ester hl750 by nhs functional group same h460 xenograft model were intravenously injected with 5 u00d7 10 9 pfu of the hl750 labeled targeting phages or control phages fluorescence imaging of mice and tissues was captured using xenogen ivis200 imaging system excitation 710 760 nm emission 810 875 nm at indicated time points and the fluorescence intensity of tissues was calculated by subtracting background using living image software xenogen the peptide was coupled to nhs peg dspe n hydroxysuccinimido carboxyl polyethyleneglycol mw 3400 derived distearoylphosphatidyl ethanolamine in a 1 1 1 molar ratio the pegylated peptide peg dspe conjugates were purified by sephadex u00ae g 15 ge healthcare gel filtration chromatography and were then dried through lyophilization the conjugates were also analyzed by hplc quantitatively and by maldi tof ms bruker microflex u2122 qualitatively a lipid film hydration method was used to prepare.