the skeleton of flavonoid compound is constructed by c6 c3 c6 and chalcone also belongs to this genus of structure generally speaking the representative active flavonoid can be divided as six groups including flavonol flavanone flavan 3 ol flavone isoflavone and anthocyanin followings are the basic skeleton in each genus of flavonoid compounds and the examples used in the present invention for analyzing whether each genus of flavonoid compounds could inhibit the activity of hasmcs proliferation the proliferation and cell viability of hasmcs were determined using wst 1 reagent roche applied science penzberg germany wst 1 is a tetrazolium salt that will be cleaved to formazan by mitochondrial dehydrogenases in viable cells first hasmcs were seeded into 96 well plates after the density reaching 70 confluence the cells were u201c synchronized u201d for 24 hours by replacing the dulbecco 39 s modified eagle medium dmem as the flash dmem supplemented with 0 5 fetal bovine serum fbs hasmcs were stimulated with 30 u03bcg ml ox ldl with or without the addition of flavonoid compound for 24 hours at the end of incubation wst 1 was added into cell medium and the amount of formazan was measured at a wavelength of 450 nm by an elisa reader the experimental method that proliferation and cell viability of hasmcs were determined using wst 1 would be performed in the present invention and the same experimental procedures would not be repeatedly illustrated in the followings please refer to fig1 which is diagram showing that the various flavonoid compounds 5 u03bcg ml inhibit the activity of hasmcs proliferation induced by ox ldl 30 u03bcg ml for 24 hours incorporating the structural formulas of the aforementioned flavonoid compounds compound 1 catechin and genistein had significant inhibition activity and were non.