40 u00b0 c flow rate 25 ml min uv detection 210 nm method 9 preparative hplc column sunfire c18 5 u03bcm 19 mm u00d7 150 mm mobile phase a aqueous 0 2 strength trifluoroacetic acid mobile phase b acetonitrile gradient 0 0 min 95 a u2192 8 min 50 a u2192 8 01 min 95 a u2192 12 min 95 a rt flow rate 25 ml min uv detection 210 nm method 10 preparative hplc column sunfire c18 5 u03bcm 19 mm u00d7 150 mm mobile phase a aqueous 0 2 strength trifluoroacetic acid mobile phase b acetonitrile 0 min 90 a u2192 13 min 90 a oven 40 u00b0 c flow rate 25 ml min uv detection 210 nm method 11 preparative hplc column xbridge c18 5 u03bcm 19 mm u00d7 150 mm mobile phase a aqueous 0 2 strength formic acid mobile phase b acetonitrile 0 min 75 a u2192 6 min 75 a rt flow rate 25 ml min uv detection 210 nm method 12 preparative hplc column xbridge c18 5 u03bcm 19 mm u00d7 150 mm mobile phase a aqueous 0 2 strength formic acid mobile phase b acetonitrile 0 min 93 a u2192 4 min 93 a rt flow rate 25 ml min uv detection 210 nm method 13 preparative hplc column xbridge c18 5 u03bcm 19 mm u00d7 150 mm.