to chromatography purification to obtain highly pure protein d the final bulk product is obtained after pooling of the chromatographically purified fractions containing the desired proteins followed by diafiltration and e or sterile filtration steps the above steps of invention are more clearly depicted in the following examples for some recombinant and cell culture proteins the examples provided herein are only for the explonation of the invention in detail and is not to be construed that the provided examples limits the scope of the present invention varying options which are within the scope of the invention but are not covered in the description that are available to the persons skilled in the art are to be taken as included in the present invention the cell lysate after fermentation is subjected to centrifugation and the insoluble fraction is treated with detergent the supernatent after centrifugation was either subjected to aerosil adsorption and desorption traditional technology table 1 or to primairy capturing of hbsag by a batch procedure in which salts of divalent cations such as calcium magnesium and zinc are added at 0 2 to 10 w v in the presence of phosphates chlorides or acetates to form white insoluble matrix the insitu formation of the matrix further interact with the antigen and this process of protein capturing is referred as himax technology table 20 this matrix was separated by centrifugation between 7000 g to 10 000 g and bound antigen was desorbed repeatedly with this buffer of ph 8 5 the desorbate was further purified using an anion exchange matrix namely the deae the hbsag activity in all the intermediate steps is given in.