the denim or other fabric clothing items are added to the machine according to the machine capacity per the manufacturer 39 s instructions typically the clothes are added prior to introducing water into the drum but the clothes can be added to water in the machine or to the pre diluted treatment composition the clothing is contacted with the treatment composition and agitated in the machine for a sufficient period to ensure that the clothing has been fully wetted by the treatment composition and to ensure that the cellulase enzyme has had an opportunity to cleave cellulose in the fabric material at this time if the treatment composition is to be reused it is often drained from the tub and saved for recycle if the treatment composition is not to be reused it can remain on the clothing for as long as needed to produce color variation such treatment periods are greater than 5 minutes greater than 30 minutes and up to 720 minutes depending on amount of enzyme during all or part of the mechanical machine action used to produce in the cellulase treated fabric the variations in color density we believe that there is an interaction between the cellulase modified fabric and mechanical tumbling or action which removes cellulose from the fabric surface and the indigo dye to create a variation in color density from place to place on fabric panels and seams further the action of the enzyme appears to cause puckering in the seams and a creation of a soft.