sulphate the reaction mixture was evaporated under vacuum and checked using tlc using chloroform ethylacetate triethylamine 47 47 8 the r f value was determined to be 0 53 the crude product was purified by column chromatography silica gel 230 400 mesh using a solvent system of chloroform ethylacetate triethylamine 47 47 6 the pure fraction monitored by tlc was combined and concentrated on a rotary evaporator pure foamy product was obtained having a yield of 400 mg synthesis of 2 u2032 3 u2032 5 u2032 tri hydroxy 2 u2032 3 u2032 4 u2032 5 u2032 5 u2033 penta deuterium u03b2 d ribofuranosy n6 benzoyl adenosine compound having structure xxvii the preceding tribenzoyl compound 2 u2032 3 u2032 5 u2032 tri o benzoyl 2 u2032 3 u2032 4 u2032 5 u2032 5 u2033 penta deuterium u03b2 d ribofuranosyl n6 benzoyl adenosine xxvi 400 mg was placed in pyridine 4 8 ml and methanol 1 2 ml the mixture was stirred to bring the compound to solution the solution was then cooled to 0 u00b0 c with an ice bucket outside to the solution 2n naoh 1 16 ml was added and the basic reaction mixture was hydrolyzed to remove o benzoyl groups for a period of 20 minutes to the reaction mixture was then added 2n hcl cooled to 0 u00b0 c the addition was done carefully to neutralize the basic solution to ph.