with suction to collect the filtrate an aliquot of 0 1 cm 3 was placed in a test tube 0 1 cm 3 solution of 3 5 dinitrobenzonitrile in acetonitrile was added and used as an internal standard 0 1 cm 3 of 40 mol m 3 solution of 1 naphthyl isothiocyanate in pyridine was added and the mixture was allowed to react in a constant temperature water bath at 50 u00b0 c for 1 hour after the reaction an aliquot of 10 u03bcl was subjected to identification and analysis of glucosamin the same hplc was carried out to identify and analyze glucosamine the analytical conditions for hplc was described as in example 2 the peak area ratio of glucosamine hydrochloride to the internal standard was substituted in the glucosamine hydrochloride calibration curve of the peak area ration of glucosamine hydrochloride to the internal standard to obtain grams of glucosamine by intrapolation method and converted to glucosamine concentration glucosamine content and its yield by converting to carbon source used in the fermentation culturing and finally converted against working days to obtain productivity these results were shown in table 3 as shown in table 3 conditions for fermenting aspergillus sp bcrc 31742 to produce glucosamine described in reference 1 was compared with those used in this example when result obtained by culturing aspergillus sp with gp medium to produce glucosamine as described by hsieh et al in 2007 glucosamine concentration was 2 31 g dm 3 is compared with the result obtained by using the medium described in this example it is seen that under identical microorganism fermentation conditions wp medium used in this example produced a glucosamine concentration 3 91 g dm 3 at.