were grown in liquid media using cell culture flasks with constant slow shaking 60 rpm min in a humidified atmosphere at 37 u00b0 c and 5 co 2 the following liquid media were tested for the growth of bartonella henselae brain heart medium bh lb broth bartonella alpha proteobacteria growth medium bapgm supplemented with 10 vol fetal calf serum fcs sigma aldrich munich germany instead of 5 vol defribrinated sheep blood endothelial cell growth medium containing supplement mix promocell heidelberg germany and schneider 39 s medium in some experiments the liquid media were supplemented with 10 vol fcs the liquid media were inoculated with 1 u00d7 10 5 or with 1 u00d7 10 6 bacteria ml and the respective optical densities od 600 nm were assessed at 24 h intervals in triplicates additionally the number of cultivated viable bacteria were determined by plating serial dilutions on cba plates followed by an incubation period of 3 weeks schneider 39 s medium schneider 39 s drosophila powder medium revised serva heidelberg germany catalogue no 47521 is composed as follows for 1 l of medium approximately 800 ml distilled water was placed in a volumetric flask 24 45 g of the powder medium was added while stirring for a better dissolving the solution can be heated to 35 u00b0 c 0 4 g sodium bicarbonate and 0 6 g calcium chloride are each dissolved in approximately 10 ml distilled water and were slowly added to the medium while stirring a ph value of 6 5 was adjusted with 1 n naoh or 1 n hcl while stirring fill up with distilled water.