amount of sec needed to obtain a desired level of freeness a pulp produced by the methods described herein can be used in the production of paper products including for example cardboard paper towels newspaper and hygiene products the methods described herein can also be suitable for textile manufacturing the commercial enzyme product celluclast 1 5l u2122 was tested for several enzymatic activities and was found to have several different types of activities table 1 list all relevant and significantly measurable activities and protein concentration carboxymethyl cellulase cmc activity equivalent to endo u03b2 glucanase activity was determined following the cmc method described in measurement of cellulase activities by t k ghose pure amp appl chem vol 69 no 2 pp 257 268 1987 the amount of reducing sugars released from enzymatic hydrolysis of a 2 solution of a well characterized cmc during a 30 0 minute hydrolysis at ph 4 8 and 50 u00b0 c is used to determine the enzymes eg activity sugar concentration is determined by the well known 3 5 dinitrosalicylic acid dns solution method described by g l miller analytical chem no 31 p 426 1959 the addition of the dns solution to the hydrolysis filtrate stops the reaction the mixture was boiled for 5 0 minutes to allow for color formation after cooling the absorbency is measured at 540 nm and the concentration is determined against a standard curve mannanase activity was determined following the method describer by m.