flask equipped with a magnetic stirrer and fixed with a separatory funnel containing 9 35 ml 128 mmol of thionyl chloride and a water condenser is placed 24 90 ml 80 mmol of linoleic acid addition of the thionyl chloride is completed with heating to about 40 u00b0 c over the course of about 40 minutes when addition of the thionyl chloride is complete the mixture is heated and stirred for an additional 50 minutes the water condenser is then replaced with a distillation side arm condenser and the crude mixture is distilled the crude distillate in the receiving flask is then fractionally distilled to obtain the acyl chloride 9z 12z octadeca 9 12 dienoyl chloride separately in a single necked round bottomed flask equipped with a magnetic stirrer 7 87 g 60 mmol of creatine is dissolved in 600 ml of water to this is added 78 ml of 1m ammonium hydroxide with vigorous stirring until heat production ceases at this point the water is removed by evaporation to yield the carboxylate salt ammonium 2 1 methylguanidino acetate shown below finally in a dry 2 necked round bottomed flask fixed with a separatory funnel containing 17 93 g 60 mmol of the prepared 9z 12z octadeca 9 12 dienoyl chloride and side arm water condenser fixed with a dry receiving flask is placed 10 66 g 72 mmol of ammonium 2 1 methylguanidino acetate the round bottomed flask is placed in an ice bath and the 9z 12z octadeca 9 12 dienoyl chloride is added drop wise after addition is completed the mixture is shaken and the ice bath is replaced by a heating mantle the flask is then heated until no more.