0 u03bcl of assay buffer was added in the next aliquot and mixed and finally 9 0 u03bcl of 324 miu ml htsh standard and 1 0 u03bcl of assay buffer was added and mixed then 25 u03bcl samples from each test tubes were pipetted together with 1 0 u03bcl of labeled antibody 40 u03bcg ml in 50 mm tris hcl buffer ph 7 7 containing 0 05 nan 3 0 9 nacl 0 5 bsa 0 05 bovine gamma globulin and 0 01 tween 20 into the miniature adhesive tape wells after 15 minutes incubation the chip was washed with running wash solution 50 mm tris hcl ph 7 8 containing 0 09 nan 3 and 0 05 tween 20 and the tape was removed and replaced by a pdms slide with a thickness of 2 0 mm and a circular hole through the pdms chip providing the final measuring cell which was clamped onto the echip and 200 ml of measuring buffer was added 50 mm na 2 b 4 o 7 0 1 nan 3 0 003 tween 20 ph 7 8 adjusted with h 2 so 4 finally the hecl intensity from each working electrode was measured in turns using the same measuring parameters as in example 1 the results are presented in fig2 c reactive protein hcrp and thyroid stimulating hormone htsh were determined simultaneously one of the working electrodes was coated with both of the catching antibodies two of the working electrodes were coated with htsh catching antibody and the rest two with hcrp catching antibody anti htsh and anti hcrp were labeled with an isothiocyanate derivative of.