capan 1 spheres and adhesive capan 1 cells among them 55 spots which were increased more than two times in hpac spheres as compared to adhesive hpac cells were detected 41 spots in the 55 spots were subject to maldi tof to identify pancreatic cancer stem cell related secretory protein table 2 proteins including hsp90ab1 aldh and vimentin which have been reported to be associated with cancer stem cell were identified these results suggest that the secretory proteins described in table 1 have potential to be pancreatic cancer stem cell related secretory proteins expression verification of pancreatic cancer stem cell related secretory proteins in sphere cell media through western blot in order to verify expressions of pancreatic cancer stem cell related secretory proteins identified by maldi tof analysis the media of adhesive hpac cells hpac spheres adhesive capan 1 cells and capan 1 spheres were subject to western blot it was verified that 5 secretory proteins including glrx3 were increased in hpac spheres and capan 1 spheres verification on expressions of mrna and protein of glrx3 in pancreatic cancer cell lines to verify glrx3 expression mrna and protein were extracted in eight pancreatic cancer cell lines aspc 1 bxpc 3 capan 1 capan 2 cfpac 1 hpac panc 1 and miapaca 2 and hpde human pancreatic ductal epithelial cell as a result it was determined that both of mrna and.