baffles the composition of the 2 u00d7 ynb 1 5 is 0 28 g yeast nitrogen base 1 0 g ammonium sulfate 1 5 g glycerol and 100 ml water the cultures were incubated for about 24 h at 37 u00b0 c with 140 rpm shaking until an o d 600 of 3 5 is reached 2 u00d7 450 ml of autoclaved syn6 1 5 glycerol media in 2 u00d7 2000 shake flasks with baffles were inoculated with 20 50 ml of each of the above preculture the cultures were then incubated for 36 h at 30 u00b0 c and 140 rpm the composition of the syn6 1 5 glycerol medium is nh 4 h 2 po 4 u2014 13 3 g mgso 4 u00d7 7h 2 o u2014 3 0 g kcl u2014 3 3 g nacl u2014 0 3 g glycerol u2014 15 0 g in water 1000 ml the media was further supplemented with cacl 2 microelements vitamins and trace elements 10l of syn6 medium composition as of syn6 1 5 glycerol but not containing glycerol but containing 1 soya oil was autoclaved in a fermentor for 20 min at 121 u00b0 c after autoclaving the temperature ph aeration and agitation were set to the desired values ph to 4 0 agitation to 400 rpm aeration to 1 vvm the fermentor was inoculated with the seed culture.