to a bottom surface of a filter membrane of a culture dish to plant hbvps and has on the bottom surface filling a suspension liquid of human brain microvascular endothelial cells into a top surface of the filter membrane to plant hbmecs on the top surface placing the culture dish in a well plate containing a culture medium and placing the well plate in a carbon dioxide incubator replacing the culture medium with a condition medium and replacing the condition medium once daily for a plurality of days thereby is constructed an in vitro human bbb model of high medical research availability application_number US 201313974561 A description after subculture hbmecs hbvps and has are further cultured in a porous filter membrane of a culture dish such as a transwell hbvps and has are cultured on the bottom surface of the porous filter membrane and hbmecs are cultured on the top surface of the porous filter membrane after culture hbmecs interconnect closely to form tight junctions tjs functioning as bbb in the present invention hbmecs are modulated by hbvps and has to improve the integrity of the in vitro bbb model and increase the activity of p glycoprotein p gp the present invention uses hbmecs and different ratios of hbvps and has to construct a bbb model and measures teer.