daughter relationships between a single cell 39 s progeny for several generations adherent cells adherent cells are lifted from bulk culture with trypsin and then seeded in the traps using the same method as the suspension cells however shortly after loading these cells will begin to adhere to the channel surface as these cells grow and divide their progeny will remain adherent and thus not flow downstream as in the case of a suspension cells in this case we still conduct time lapse microscopy to determine cell cycle timing as well as lineage information however after a few rounds of division we introduce trypsin to the cells via the bypass channel in order to detach them from the channel surface by applying a slight pressure drop across the traps during this trypsinization we are able to flow these lifted cells downstream for secondary capture by imaging this process we can maintain the lineage information gathered by time lapse microscopy and once the cells are passed downstream can continue the proliferation measurements in order to effectively grow suspension cells in these devices without significant sticking we passivate the channel surfaces with pll g peg whereas for adherent cell growth we use.