buffers subsequently briefly dried and released once more from the glass fiber material after the addition of water or 10 mm tris hcl the detergent is not particularly limited nonionic detergents are preferred detergents such as tween 20 tween 80 triton x 100 etc optionally high concentration of at least 10 vol or a mixture of detergent alcohol also again high concentration of detergent can be used as nonionic detergent the detergent can be used in an amount of from 5 to 50 vol preferably 10 40 vol more preferably 10 20 vol if a salt of a polyvalent cation is added it may be possible to use a lower amount of detergent compared to the amount used if no salt of a polyvalent cation is added the low salt buffer used can be a known wash buffer an known elution buffer a buffer that acts as wash and elution buffer and combination of a wash buffer and an elution buffer a known low salt buffer is for example a 10 mm tris hcl this means that contrary to the previously described mechanisms and methodological solution approaches adsorption of nucleic acids to known mineral support materials can also be carried out in principle without salts in this way it is possible to carry out the lysis of complex biological samples with a simple and above all universally employable lysis buffer without the previously required high salt components if larger amounts of starting materials are used for the isolation of nucleic acids it then becomes clear however that the purity of the isolated nucleic acids measured as ratio 260 280 is often inadequate with the combination of the described lysis buffer.