the analysis is working properly both of these situations can be avoided by a particular use of the described invention fig9 shows a sample cell 18 where three liquids are introduced the sample containing the unknown concentration of analyte the reagent containing the marker and a standard of appropriate concentration baffles 19 may be used to prevent complete mixing of the constituents when the chamber has equilibrated as previously described readings along line 21 are used to generate a standard curve using the previously described method and readings along line 20 are used to find the appropriate sample dilution for the analysis thus a simultaneous standard curve and sample analysis can be performed in the same reaction chamber which ensures that the reaction conditions for the sample and standard are identical more than one sample could be run in a single chamber by altering the geometry as long as the appropriate mixing occurs what is being measured is light per pixel of the area scanned an agglutination assay is performed in the test chamber as described with the following features added to affect a serologic assay fig1 is a schematic cross sectional view of a test chamber having at least one transparent surface 101 of the general construction described above to one surface of the chamber are adhered particles 102 whose surfaces express or contain the antigen 103 to which the target antibody is directed the particles may be artificial such as latex latex styrene styrene polycarbonate or the like with antigen bonded to the surface by any of several means well known to the art or they may be natural such as pollen bacteria.