dodecyl sulphate polyacrylamide gel electrophoreses sds page and blotted onto a pvdf membrane immobilon millipore bedford mass usa membranes were incubated with sv40t antibody 1 400 dilution santa cruz biotechnology santa cruz calif usa and gapdh 1 5 000 dilution abcam cambridge uk as a house keeping antigen followed by incubation with goat anti mouse hrp conjugate dako and visualization using pierce ecl western blotting substrate thermo fisher scientific waltham mass usa cellular homogenates matured for 10 days at 37 u00b0 c were analyzed as described above using 6 or 12 sds page as indicated using the following antibodies rabbit anti aquaporin 1 aqp1 1 4000 12 chemicon intl millipore rabbit anti oct2 1 500 12 alpha diagnostics san antonio tex usa rabbit anti cd26 dipeptidyl peptidase iv dpp iv 1 200 12 santa cruz biotechnology rabbit anti multidrug resistance protein 4 27 mrp4 abcc4 1 5000 6 mouse anti pgp 1 200 6 dako goat anti mouse hrp conjugate dako and goat anti rabbit hrp conjugate dako human kidney homogenate in ripa buffer was used as control the ability of ciptec 14 4 to reabsorb albumin was investigated by the incubation of confluent monolayers in 24 well plates with 50 u03bcg ml bsa fitc sigma aldrich for 30 min at 37 u00b0 c unless described otherwise uptake was arrested using ice cold pbs and cells were detached using trypsin fixed by paraformaldehyde 0 5 in pbs and analyzed using flow cytometry or immuno fluorescence microscopy concentration and temperature dependent uptake was investigated using a concentration range bsa fitc 0 3 7 11 33 100.