or bolus may be in the same extremity as testing will be in order to improve testing due to higher concentrations of spirochete numbers at the provocation site if an infection is present the anterior of the lower arm is ideal in another embodiment the administrator may place the recessed needle injection platform pad on the arm gently but firmly and then trigger the top plunger to release the spring for a single use disposable injection of sge as a borrelia provocation procedure fig7 provides a flow chart showing steps of an embodiment of testing the method begins with the obtaining the salivary gland extract sge the sge may apply to either creating purifying or otherwise extracting or generating the sge or may simply refer to loading a syringe or injection device with pre prepared sge the obtained sge may then be injected into a patient test subject this may be a human or other mammal depending on application once injected there is a waiting period to provoke the lyme borrelia to leave its preferred tissue and enter the blood stream of the patient after this waiting period blood may be drawn and tested for the presence of the lyme borrelia a presence of it indicates that an infection is present while a lack of it indicates that there is likely no infection fig8 provides a flow chart showing steps of an embodiment of treatment the method begins with the obtaining the salivary gland extract sge the obtaining step may apply to either creating purifying or otherwise extracting or generating the sge or may simply refer to loading a syringe or injection device with pre prepared sge the.