the strains escherichia coli bl21 de3 e coli rv308 e coli dh5 u03b1 e coli jm109 e coli xl 1 or klebsiella planticola rf this is because these are important strains for molecular biological operations on genes for instance for cloning see examples and additionally important producer strains as alternative thereto in each case a microorganism which is a gram positive bacterium is preferred in particular one of the genera bacillus staphylococcus or corynebacterium very especially of the species bacillus lentus b licheniformis b amyloliquefaciens b subtilis b globigii or b alcalophilus staphylococcus carnosus or corynebacterium glutamicum and among these very particularly preferably b licheniformis dsm 13 this is because these are particularly important for the biotechnological production of valuable products and proteins because they are naturally able to secrete them into the surrounding medium on the other hand they are increasingly related to the b licheniformis employed for the present application so that the working steps described and derived from the sequences disclosed in each case should proceed more successfully as the extent of relationship to b licheniformis dsm 13 increases it is thus to be assumed for example that a gene indicated in the sequence listing can after point mutation be used in a related species directly for a deletion mutation without the need to isolate the homologous gene from the strain itself for this purpose the present invention is aimed in particular at improving fermentation.