a 1 wt aqueous methylcellulose solution the suspension was administered orally to beagles n 6 which had been subjected to fasting overnight as a result the compound b concentration of plasma over time was as shown in fig5 the difference in absorption among the samples was evaluated by comparing the area under the plasma drug concentration time curve auc the plasma drug concentration in the collected blood was quantitatively determined according roughly to the following method blood about 0 7 ml collected from the cephalic vein was centrifuged 4 u00b0 c about 9000 u00d7 g 10 min in the presence of heparin to obtain plasma methanol 400 u03bcl and an internal standard substance solution sodium salt of 7 methyl 4 5 h 10 dioxo 2 h 1 2 3 triazolo 4 5 c 1 benzazepine 100 ng ml 100 u03bcl were added to the plasma 100 u03bcl and the mixture was stirred and centrifuged 4 u00b0 c about 9000 u00d7 g 10 min the supernatant was evaporated to dryness at room temperature under a nitrogen gas stream and 150 u03bcl of a mixed solution composed of a 10 mmol l phosphate buffer ph 7 0 and a methanol 8 2 was added to the residue for.