yogurt and cheese would not be inhibited by the use of the described preparations to generate the reactive oxygen species of the present invention a 10 ml volume of milk was used a model to test the hypothesis that a chloroperoxidase enzyme could be used instead of lactoperoxidase to generate antimicrobial reactive oxygen species milk was again spiked with e coli u02dc 10 7 cells ml a suitable concentration of both beta galactosidase 2 3 u03bcl u2267 2 600 units ml and glucose oxidase 15 u03bcl of a 2 5 mg ml solution u02dc 200 units mg was supplemented to the milk a preparation of chloroperoxidase 0 5 u03bcl u2267 11 100 units ml was then added and resulted in a complete eradication of e coli cells within 24 hours at 37 u00b0 c control experiments without each or two of the enzymes resulted in the proliferation of a bacterial numbers within the same incubation conditions 10 8 9 cells ml a field trial was conducted using a supplemented glucose based enzymatic system to produce the reactive oxygen species and to assess the efficacy of such an approach to producing the antimicrobial agents a number of cows 8 were treated twice a day.