reared at 27 u00b0 u00b1 1 u00b0 c and 65 u00b0 u00b1 5 relative humidity under a photoperiodic regime of 12 12 light dark z subfasciatus and a obtectus were reared on a diet of dried pinto beans r dominica and s oryzae were reared upon a diet of 96 2 2 w w soft white wheat whole wheat flour brewers yeast quantification of linalool from ocimum canum leaves of ocimum canum were collected form the butare prefecture in rwanda air dried according to traditional practice and express shipped to montana state university where they were stored in a 20 u00b0 c freezer prior to bioassay and chemical analysis the leaves 1 0 g 4 replicates were milled in a waring blender for 2 minutes and extracted immediately in 60 ml of 1 4 isopropanol hexane containing 40 ng u03bcl decane as an internal standard in a 125 ml erlenmeyer flask for 24 hours the flasks were covered to prevent photodegradation and occasionally agitated the resulting solution was directly injected into a gas chromatograph for quantitative analysis all solvents were purchased from the sigma chemical co st louis mo gas chromatography was performed on a varian model 3700 equipped with a flame ionization detector containing a 50 m u00d7 0 25 mm i d hp 1 column with a 0 11 u03bc film thickness he carrier gas velocity was 31 cm 3 s 220 u00b0 c temperature program initial temperature 60 u00b0 c initial hold 8 min temperature increase 4 u00b0 c per minute final temperature 260 u00b0 c the linalool peak.