is 200 u03bcl sample error handling at the end of the sample pipetting step a custom dialog appears to inform the user that samples were not well pipetted user has 4 options a transfer samples with error manually u2014 those samples will be considered for next pipetting steps b skip samples with error u2014 those samples will not be considered for next pipetting steps c retry samples with error u2014 lh will retry to pipette those samples again d abort the method 15 custom dialog appears for putting a seal mat on intermediate plate 1 an email is sent to the user 16 intermediate plate 1 is shaken for 30 seconds at 1200 rpm 17 incubation occurs for 15 minutes in the cpac 2 in certain embodiments incubation occurs at 4 u00b0 c 18 process in parallel with step 17 balance plate 1 is filled with water volume per well can be 350 u03bcl 300 u03bcl or 0 u03bcl depending if the equal well in intermediate plate 1 has reagent 1 and sample only reagent 1 e g if sample was discarded in a previous step or it is empty no sample to prepare in that well 19 custom dialog appears for centrifugation and plate exchange in cpac 1 intermediate plate 1 must be centrifuged for 10 minutes at 6168 times gravity at 10 u00b0 c on manual centrifuge or 30 minutes at 1000 times gravity at rt with an agilent vspin sample rack must be replaced for intermediate plate 2 an email is sent to the user 20 custom dialog appears for removing the seal mat from intermediate plate 1 an email is sent to the user 21 transference of 150 u03bcl supernatant from intermediate plate 1 to intermediate plate 2 in certain embodiments tips.