35 80 20 the fraction eluted with 65 methanol was prepared by rp 18 hplc 70 methanol and the chromatography peak at 40 min rt was collected then drying the collection under reduced pressure to get ppd compound 100 mg phoysicochemical parameters of steroidal saponin compounds mpd and ppd synthesized in example 1 and 2 white powder mp 230 233 u00b0 c dec u03b1 25 d u2212 88 7 u00b0 c 0 80 pyridine ir max 3400 3450 oh 2950 1380 1040 glycosyl c u2014 o fab ms 1085 m na 1062 m h 1031 m h u2014 ch 3 oh 869 m u00d7 h u2014 ch 3 oh glc 723 m h u2014 ch 3 oh glc rha 577 m h u2014 ch 3 oh glc rha u00d7 2 415 m h u2014 ch 3 oh glc u00d7 2 rha u00d7 2 397 m h u2014 ch 3 oh u2014 h 2 o glc u00d7 2 rha u00d7 2 1 h nmr c 5 d 5 n u03b4 0 87 3h s ch 3 18 0 98 3h d ch 3 27 1 08 3h s ch 3 19 1 03 3h d ch 3 21 1 26 3h d j 6 2 hz 1 28 3h d j 6 2 hz 13 c nmr data please see table 2 ir max 3420 oh 2940 ch 1645 1450 1375 1335 1225 1115 1070 1045 920 890 esi ms 1053 m na 1029 m u2212 h u2212 883 m u2212 h u2212 146 u2212 737 m u2212 h 146 u00d7 2 u2212 1 h nmr c 5 d 5.