utilization of the barrier device of fig7 is shown high containment transfer cup assembly 6 whose interior is in a sterile state and carrying flexible tube 7 which is internally sterile is offered to the barrier guide tube 12 it is pushed therethrough by means of handle 6j and docking thereof at the active frame 11 and provision of communication between tube 7 and the interior of the sterile suite are achieved by rotation of handle 6j about its longitudinal axis in fig1 and 12 frame 11 can be seen to carry at a hinge 22 a bar 21 formed at its distal end with a clasp 23 engageable by a latch assembly 24 mounted on the frame 11 opposite the hinge 22 the bar 21 carries a bracket 25 to which is attached the door 10 which is a male fitting the male door has a peripheral surface shown here at 26 which is conical reducing in diameter towards its forward end and matching a like shaped internal surface 27 of the frame 11 at its front surface the door is provided with a plurality of holding cams 28 arranged around a circle at intervals as mate members and able to enter arcuate channels 48 as female members in a door 6a belonging to a docking unit of assembly 6 this enables the doors to engage disengage through appropriate rotation of the transfer vessel relative to the storage unit an intimate engagement between doors is achieved and ingress of material between door to contaminate their external surfaces is prevented by corner seals 6b and 49 carried by the respective doors frame 6c secured around the mouth of.