a rotator overnight and then spun down in a centrifuge the liquid was decanted off and the solid was washed with three 10 ml portions of dmf the resultant coated material included a free carboxylic acid group a 15 ml centrifuge tube with a cap was charged with the gold coated smco 5 particles having free carboxylic acid groups thereon from example 2 1 hydroxybenzotriazole hobt 2 1h benzotriazole 1 yl 1 1 3 3 tetramethyluronium hexafluorophosphate hbtu 0 5 m in dmf 3 ml and diisopropylethylamine 2 0 m in n methylpyrrolidone 1 5 ml the mixture was placed on a rotator for 30 minutes and then spun down in a centrifuge the liquid was decanted off and the solid was washed with three 10 ml portions of dmf n n dimethylformamide 5 ml was added to the solid followed by addition of fmoc ethylenediamine hydrochloride 2 27 g 8 04 mmol diisopropylethylamine 2 0 m in n methylpyrrolidone 5 ml the mixture was placed on a rotator overnight and then spun down in a centrifuge the liquid was decanted off and the solid was washed with three 5 ml portions of dmf the particles were dried overnight under a high vacuum the fmoc label was cleaved using piperidine and monitored by uv to give the loading factor i e the amount of accessible fmoc groups per gram of material the loading factor using this procedure.