on chip bioparticle separator as shown in fig2 a in accordance with an embodiment of the present invention the shape of the series of pressure pulses is an approximately triangular pulse which consists of magnitude 204 a rising section 206 a falling section 208 the on time of the pulse 210 the peak to peak duration between the pulses 202 and the number of the pressure pulses 212 in accordance with an embodiment of the present invention pressure pulses with magnitude 204 of approximately 5 kpa to 50 kpa kilo pascal and peak to peak duration 202 of approximately 20 msec to 200 msec milliseconds are used residual pressure between each pulse is bled off in this duration for a sequence of 10 to 50 pulses the desired separation can be achieved in 5 to 150 sec in accordance with another embodiment of the present invention the shape of the series of pressure pulses is rectangular which consists of the pressure magnitude 216 the duration of the pulses 214 the number of the pressure pulses 222 and the same and or different on time of pulse 218 220 as shown in fig2 b in yet another embodiment a more generic.