the cox model all statistical analysis were done using the procedures of the spss 10 02 package several parameters were selected for investigation cd1a and langerin two markers of langerhans type immature dc cd123 a marker of pdc and dc lamp a molecule expressed specifically by mature dc de saint vis et al 1998 immunity 9 3 325 36 were analyzed immunostaining was also performed for analyzing the expression of chemokine receptors ccr6 and ccr7 and of their ligands mip3 u03b1 and ccl19 mip 3 u03b2 or hccl21 6ckine which are known to drive immature and mature dc migration respectively dieu et al 1998 j exp med 188 2 373 86 in addition cd3 lymphocytes and cd68 macrophages infiltrates were studied table 1 describes the presence and phenotype of immune cells as well as the chemokine and chemokine receptor expression pattern in the 1996 series of 152 patients with non metastatic breast cancer tumors 112 tumors from this series were infiltrated by dendritic cells as can be seen in table 1 56 of the tumors contained dc lamp mature dendritic cells stewart 1995 lancet 346 8978 796 8 which were consistently located within cd3 t cell infiltrates indeed a strong correlation between dc lamp expression and cd3 t lymphocytes infiltrates was observed r 0 73 p lt.