recorded when an animal moves all four paws into the arm as described in simonin et al 1998 embo j 17 886 behavior is scored by an observer and or via a video camera over a 5 minute test session a greater amount of time spent or entries made by the animal in the open versus the closed arms is an indicator of anxiolytic activity the elevated zero maze is a modification of the elevated plus maze the elevated zero maze consists of a plexiglas apparatus in the shape of a circle i e a circular runway of 46 cm diameter and 5 5 cm runway width with two open and two wall enclosed sectors of equal size it is elevated up to a meter above the ground this apparatus is described in simonin et al supra and crawley supra complete protocol details can be found in kathuria et al 2003 nature medicine 9 76 briefly 30 minutes following intraperitoneal administration of test compound or control an animal is placed on one open sector in front of an enclosed sector time in a new sector is recorded as entry with all four paws behavior will be scored by an observer and or via a video camera over a 5 minute test session a greater amount of time spent or entries made by the animal in the open versus the walled sector is an indicator of anxiolytic activity compounds can be tested to determine if they.