polygalacturonase of erwinia carotovora expressed in e coli as prepared in example 4 the other pectinase used was novozyme pectinase polygalacturonase from aspergillus niger the following general method was used a reaction mixture containing 30 mg of treated fiber and 1 u of recombinant pectinase or 20 u03bcl diluted novozyme pectinase 50 u00d7 dilution in 400 u03bcl of a buffer 100 mm sodium citrate ph 5 0 for recombinant pectinase ph 4 5 for novozyme pectinase was heated at 40 u00b0 c after 1 hr 50 u03bcl of the reaction solution was removed and was added into 1 ml of 10 mm naoh to stop further hydrolysis the amount of reducing sugar was determined with a well established method involving the hydroxylbenzoic acid hydrazide reagent hbah lever 1972 analytical biochem 47 273 279 the disclosure of which is herein incorporated by reference after hbah treatment the solution turned yellow the quantity of reducing sugar can be determined though the reading of o d at 420 nm and read against a standard curve with o d versus known quantities of galacturonic acid table 1 provides results it is evident from table 1 that an extraction process of the present invention is effective at degumming hemp fiber according to analysis by the recombinant bacterial erwinia carotovora pectinase.