000 00048 1 100 000 000erythrose 0 36 000 4 lt 10 8 lt 1024 lt 1048 lt 10glyceraldehyde 0 23 000 4 lt 10 8 lt 1024 lt 1048 lt 10xylose aldrich 0 39 000 4 110 000 8 200 00024 460 000 00048 570 000 000xylose roquette 0 26 000 4 123 000 8 290 00024 680 000 00048 400 000 000______________________________________ this example demonstrates the dose efficacy relationship between the concentration of inhibitory carbohydrate and bacterial multiplication using the same methodology as above 5 grams of d erythrose and 10 grams of d l glyceraldehyde were dissolved and diluted to obtain four concentrations ranging between 0 10 and 2 0 this was done by preparing 10 stock solutions and diluting them according to the following schedule 2 0 grams of 10 solution was diluted with 8 0 grams of diluent to yield 2 0 solution 1 0 gram of 10 solution was diluted with 9 0 grams of diluent to yield 1 0 solution 2 0 grams of 2 solution was diluted with 6 0 grams of diluent to yield 0 5 solution 1 0 gram of 1 solution was diluted with 9 0 grams of diluent to yield 0 1 solution water was the diluent for the disc assay and tsb was the diluent for the broth assay two strains of s mutans atcc 25175 and atcc 27351 were prepared in tsb incubated 24 hours at 35 u00b0 c before inhibition testing in the disc inhibition test.