of such immunoadhesion molecules include use of the streptavidin core region to make a tetrameric scfv molecule kipriyanov s m et al 1995 human antibodies and hybridomas 6 93 101 and use of a cysteine residue a marker peptide and a c terminal polyhistidine tag to make bivalent and biotinylated scfv molecules kipriyanov s m et al 1994 mol immunol 31 1047 1058 antibody fragments such as fab and f ab u2032 2 fragments can be prepared from whole antibodies using conventional techniques such as papain or pepsin digestion respectively of whole antibodies moreover antibodies antibody portions and immunoadhesion molecules can be obtained using standard recombinant dna techniques as are well known in the art the term u201c humanized antibody u201d refers to an antibody that is composed partially or fully of amino acid sequences derived from a human antibody germline or a rearranged sequence and made by altering the sequence of an antibody having non human complementarity determining regions cdrs the framework regions of the variable regions are substituted by corresponding human framework regions the human framework regions include genomic framework regions as well as those containing one or more amino acid substitutions in particular such substitutions include mutations in which an amino acid at a particular position in the human framework is replaced with the amino acid from the corresponding position of the natural framework for the non human cdr for example a humanized antibody having mouse cdrs may contain one or more substitutions that replace a particular.