prepared standard compounds was used through three independent experiments results were obtained and represented by a mean u00b1 sd preparation of vector for producing escherichia coli with high productivity of u03b2 carotene and retinal conventional processes involving genome dna preparation restriction enzyme cleavage transformation and standard molecular biological technologies have been executed according to description in related documents sambrook and russell 2001 pcr was performed using pfu dna polymerase according to standard protocols solgent co korea blh gene of uncultured marine bacteria 66a03 genbank accession no aay68319 was synthesized into genofocus daejeon korea according to codon optimization by gene designer software dna 2 0 menlo park usa in order to express the above gene in escherichia coli according to the present example an enzyme involved in a velocity determination process that is a gene encoding dxp synthase was additionally introduced into escherichia coli having an mep path and at the same time a gene encoding an enzyme associated with a mevalonate path was selected from a variety of gene resources and introduced thus preparing escherichia coli with high productivity of u03b2 carotene 1 preparation of psna vector including a gene encoding an enzyme in a mevalonate path associated with synthesis of ipp from a carbon source genes encoding an enzyme in a mevalonate path associated with ipp synthesis from a carbon source used in the present experiment are shown in table 1 below.