851 855 the ratio of peaks at 1417 and 1451 cm u2212 1 was used to determine the type of pani based on sers profiles and using the spectra obtained by baibarac et al as a reference pani formed on dialyzed ncss was determined to be eb on au substrate baibarac m et al synthetic met 1998 96 63 70 sers spectrum of pani made in water under x ray irradiation without ncss was also obtained fig3 b dialysis removes free ions and other compounds in ncss after synthesis but it has been reported that ag ionic species may slowly depart ag nanoparticles in solution over time kittler s et al chem mat 2010 22 4548 4554 however immediately after dialysis the solution had a limited amount of ionic species as verified by stem inspections the amount of pani and atomic absorption aa measurements aa measurements showed free ag and au ions and small nanoparticles in dialyzed ncss between 0 1 detection limit to 0 25 ppm au or ag in the supernatant after centrifugation separation of dialyzed ncss taking into consideration that 10 min of 5000 rpm centrifugation could not pull down small nanoparticles e g lt 2 nm in water the amount of free ions in the dialyzed ncs solutions should be less than the measured 0 1 ppm au or 0 25 ppm ag ions which correspond to 0 6 u03bcm au ions or 2 3 u03bcm ag ions to further investigate the formation mechanisms and the origin of the unique sers profile for dialyzed ncss we tested pani formation in solutions of ag and au ions mixed with aniline and dialyzed ncss base on ncss sers measurements no additional pani formation with up to 1 u03bcm au and ag ions added to aniline and irradiated with.