4 oh midazolam after 24 hours induction with or without 1 mm phenobarbital and 10 um dexamethasone induced non induced during the 24 hours induction dexamethasone dmso and bio was left out from the medium composition m1 after induction the activity assay was performed as described elsewhere see example 6 verification of embryonic endoderm fate of hesc cells cultured on 2d prior to seeding on 3d scaffolds induction of endoderm markers foxa2 hhex cxcr4 cer1 and downregulation of pluripotency markers sox7 nanog oct4 in hesc cells cultured in 2d format for 7 days prior to seeding on 3d scaffolds two independent cell lines sa121 and sa181 cultured according to protocol a on matrigel coated 2d surface brightfield morphology of cells is also shown verification of embryonic endoderm fate of ips cells cultured on 2d prior to seeding on 3d scaffolds induction of endoderm markers foxa2 hhex cxcr4 cer1 in ips cells cultured in 2d format for 7 days prior to seeding on 3d scaffolds cell line imr90 cultured according to protocol h exp169a and f exp169b verification of hepatic progenitor fate of hesc cells cultured on 2d prior to seeding on 3d scaffolds induction of hepatic progenitor markers afp epcam foxa2 hnf4 a krt19 krt8 in hesc cells cultured in 2d format for 11 days prior to seeding on 3d scaffolds cell lines sa121 and sa181 cultured according to protocol g baharvand h et al 2006 differentiation of human embryonic stem cells into hepatocytes in 2d and 3d culture systems in vitro.