actual signal emerges from the baseline at the point exponential growth begins a normalized amplification profile based on a ratio to the baseline can create a more uniformly scaled amplification curve across different samples filters wells dyes or machines application_number US 2208708 A description various embodiments of the present teachings relate to systems and methods for baseline correction or adjustment of rt pcr or other amplification curves signatures graphs profiles or data using a non linear or non subtractive normalization process according to various embodiments an amplification curve signature graph profile or data can be received from detection of fluorescent emissions in a rt pcr or other instrument according to various embodiments the calibration systems and methods can be implemented in or applied to rt pcr scanning systems or rt pcr imaging systems or other systems or platforms in some embodiments systems and methods according to the present teachings can be applied to non real time pcr instruments according to various embodiments rt pcr or other processing can take place using a standard sample plate such as a 96 well or other capacity microtitre well or plate in some embodiments each well or other container or location in a plate or other platform can contain samples for example samples of dna fragments or other material to which one or more spectrally distinct dye is attached for detection and analysis according to various embodiments a calibration normalization or other adjustment can be performed to normalize adjust or otherwise increase the consistency and.