antisense molecules and ribozymes in the liver the methods find use in treating hepatic diseases or conditions the methods also find use in treating any disease or condition in which systemic administration of the therapeutic substance for example a secretory protein is desired the methods involve administering to a mammalian patient having a need for liver expression of a therapeutic molecule an aav vector containing a therapeutically effective amount of the therapeutic molecule also provided are novel vectors employable in these methods application_number US 10979902 A description in murine mammalian patients the fate of aav vectors was followed after direct intravenous injection and it was surprisingly found that the aav vectors possess organ tropism for liver our aav vectors contained the lacz reporter gene or the human globin gene in mice administered the lacz reporter gene containing aav vectors expression occured in hepatocytes but a cytotoxic t lymphocyte response against u03b2gal was not detected the recombinant aav vectors when directly injected intravenously in mice accumulated predominantly in liver cells the aav recombinant virus stocks containing the cmv promoter cmv p driven lacz gene vcmvp lacz cloned in between aav inverted terminal repeats itr and the aav recombinant virus stocks containing the genomic copy of the normal human a u03b3 globin gene driven by the human u03b2 globin promoter u03b2p plus an upstream hypersensitive site 2 enhancer element cloned in between aav itr were generated from their respective recombinant plasmids by the methods described in samulski helper free stocks of recombinant adeno associated viruses normal integration does not require viral gene expression j virol 63 1989 3822 3828 nahreini versatile adeno associated virus 2 based vectors for constructing recombinant virions gene 124 1993 257 262 zhou adeno associated virus 2 mediated high efficiency gene transfer into immature.