cuttings were then dipped in 1000 ppm iba dissolved in 50 ethyl alcohol for five seconds and the base then placed in a soil less mix of 1 part vermiculite and 2 parts perlite in propagation flats flats were placed under mist with the frequency of misting regulated by an artificial leaf rooting occurred in about two to three weeks materials were propagated using hardwood cuttings current year shoots were collected in the middle of november they were cut to 14 inch long and the basal ends soaked for 24 hours in a 100 ppm iba they were then placed in moist burlap bags which were then placed in plastic bags securely closed with a wire and incubated at about 60 u00b0 f cuttings were inspected every week starting after the second week of incubation when the bases of most cuttings were covered with callus they were planted in paper sleeves with soil less mix of three parts fir bark and one part sand they were placed under cover to protect from rain and watered whenever needed materials were propagated using tissue culture the procedures involved collecting young shoots usually in april and then sterilizing them with a surface sterilizing agent such as common household bleach the shoots were then rinsed several times with sterile water cut into small pieces each containing vegetative terminal or auxiliary buds these cuttings were then placed in special media for tissue establishment they were transferred into shoot multiplication medium where auxiliary shoots proliferate in numbers dependent on the type of rootstock these multiplied shoots were.