9 gene application_number US 20485003 A description the aim of this invention is to alter the functioning of one or more of the four proton pumping multiprotein complexes of the mitochondrial inner membrane nadh reductase cytochrome c reductase and ctochrome c oxidase or the atp synthase complex by using two forms of the same gene resulting in a decrease in cellular energy levels one form would be the naturally occurring functionally edited transcript and the other a non edited transcript resulting in a different rna and putative protein and thus impaired subunit alignment and loss of function of the complex a subunit nad9 of complex i nadh ubiquinone oxido reductase is the first complex of the respiratory chain leading to the reduction of ubiquinone an electron transporter in the mitochondrial inner membrane complex i occupies a strategic position in the electron flow amp mutations that perturb its assembly have been shown to induce male sterility this subunit nad9 corresponds to the 30 kda protein of the complex i of mammals in complex i nad9 is situated in the iron sulphur sub fraction facing the mitochondrial matrix its deduced amino acid composition indicates that it is an hydrophilic protein nad9 would be the best candidate for the purpose.