toluene methanol solvent system fraction p gave 8 6 mg and fraction q 11 3 mg of pure dolastatin 10 total yield 28 7 mg of amorphous colorless powder mp 107 u00b0 112 u00b0 from methylene chloride methanol tlc table iii visualization found best by uv light or iodine vapor u03b1 d 29 68 0 c 0 01 ch 3 oh uv ch 3 oh u03bbmax 209 u03b5 8 100 216 u03b5 20 180 and 242 u03b5 3 609 nm ir kbr u03b3max 2975 2940 2890 2870 1680 1650 1540 1460 1390 1100 750 and 700 cm 1 h and c nmr see table ii and hreims tables i and iii m z 784 4890 calcd for c 42 h 68 n 6 o 6 s 784 4922 esca for sulfur one the thin layer chromatographic properties of dolastatin 10 are shown in table iii the suprisingly remarkable anticancer activity of dolastatin 10 has been summarized in table iv table iii______________________________________thin layer chromatographic propertiesof dolastatin 10sub r sub fstrate solvent system value______________________________________silica ch sub 2 cl ch sub 3 oh h sub 2 o 90 10 0 8 0 38gelsilica ch sub 2 cl sub 2 ch sub 3 oh h sub 2 o nh sub 4 oh 90 10 0 8 0 2 0 43gelsilica etoac ch sub 3 oh h sub 2 o 90 10 1 0 0 31gelsilica etoac ch sub 3 oh h sub 2 o nh.