towards the enzyme phosphatase is different than the behavior of the 2 triphopsphate ester astp asmp releases l ascorbic acid much faster than astp when treated with a purified phosphatase from potato table i in addition astp is more resistant to acid catalyzed release of l ascorbate than is asmp see 34 blank 34 reactions in table i in ph 5 0 buffer astp released no l ascorbate after 20 hr compared to about 15 released by asmp table i______________________________________enzymic hydrolysis of l ascorbate 2 phosphate asmp and l ascorbate 2 triphosphate astp using acid phosphatase from potato l ascorbatereleased during enzymolysis sup 1 substratereaction asmp astptime h blank phosphatase blank phosphatase______________________________________0 25 0 9 3 0 00 50 0 18 6 0 01 0 0 35 5 0 02 0 2 9 60 5 0 03 0 4 6 77 3 0 0 44 0 4 6 88 4 0 0 88 0 8 1 100 0 3 520 0 14 5 100 0 8 5______________________________________ sup 1 enzymolysis was done at 25 u00b0 by stirring 200 micromoles of magnesium lascorbate 2phosphate or ammonium lascorbate 2triphosphate in 100 ml of 0 05 m acetate buffer ph 5 0 containing about 400 mg of bakers 39 compressed yeast and 2 units of phosphatase 1 unit activity hydrolyzes 1 u03bcmole of pnitrophenyl phosphate at ph 4 8 and 37 u00b0 at a given reaction time an aliquot 5 ml was removed mixed with 5 0 m of 12 trichloroacetic acid 4 metaphosphoric acid and the mixture filtered an aliquot 5 ml of.