used to seed 5 lit erlenmeyer flasks containing 1 lit of the same medium prepared similarly in replicates of four at room temperature 28 30 u00b0 c the flasks were kept stationary for 15 days at the end of 15 days fungal mycelia were removed by filtration and fermentation broth was extracted with chloroform the chloroform extract 30 mg after removal of the solvent in vacuum was fractionated through a column of silica gel using petroleum ether ethyl acetate mixture initially 200 ml of ethyl acetate petroleum ether in the ratio 1 99 was used this was followed by elution with 200ml of a mixture of ethyl acetate petroleum ether 2 98 the next percentage of ethyl acetate used was 5 and petroleum ether was 95 subsequently ethyl acetate percentage was increased by 5 the sub fractions obtained were spotted on silica gel tlc plates combined and concentrated after developing and spraying with cerric sulphate the final purification of the compound was obtained by chromatography using sephadex lh 20 as adsorbent and eluting the compound with chloroform methanol 1 1 approximately 9 mg of chrysogenazine was purified as a yellow solid in determining the structure of the compound correlation spectroscopy cosy heteronuclear multiple quantum correlation hmqc heteronuclear multiple bond correlations hmbc distortionless enhancement by polarization transfer dept 1 h and 13 c nmr spectral data were obtained using a brucker avance 300 spectrometer 1 h and 13 c nmr spectra was recorded at 300 mhz.