vessel and this is allowed to stand for 6 8 hours another 25 liters of extract are withdrawn and added to the 25 liters collected above to this combined 50 liters is added 2 liters of 27 solution of zinc chloride in methanol or 27 zinc chloride in water the precipitate thus formed is allowed to sit at room temperature for 16 18 hours the precipitate is collected and dried in an oven set at 70 u00b0 c in phase two the dried precipitate from phase one is weighed and redissolved in enough of a 2 5 solution of citric acid to effect a concentration of 1 gram of precipitate to 40 ml of solution this suspension is heated to about 70 u00b0 c and filtered hot the collected hot filtrate is then combined with enough nacl usp to effect a concentration of about 3 68 grams nacl to 100 ml filtrate alternatively potassium chloride can be used in the same amount as the sodium chloride the precipitate thus formed is allowed to stand at room temperature for 16 18 hours the precipitate is filtered and placed into an oven set at 60 u00b0 c to dry or is dried in vacuo the dried precipitate is then ground weighed and packaged for further use instead of zinc chloride in phase one step three other acid salts which are soluble in methanol can be used such salts include zinc sulfate zinc iodide zinc nitrate and other zinc acid salts which are soluble in the solvent used either ethanol or methanol if water alcohol is used as the extractant then sodium chloride may be used as the precipitating agent one kg of bloodroot is extracted with 3 0 liters of acidulated methanol 1 hcl.