for treatment of atopic dermatitis another humanized antibody suitable for treatment of atopic dermatitis is e25 rhumab e25 produced by genentech inc this antibody is described in presta et al j immunol 151 2623 2632 1993 tes c21 and tesc 2 were produced and screened as follows briefly male balb c mice were immunized several times with polyclonal human ige from sera provided by ventrex the ige was combined with a suitable adjuvant mice were sacrificed after the last injection of immunogen and the spleens were removed for preparing single cell suspensions for fusion with myeloma cells the spleen cells were fused with sp2 0 cells using a fusion mixture of polyethylene glycol 1450 kodak cmf pbs and dmso dmem was added after the cell suspensions were combined the hybridomas resulting from the fusion were then screened by enzyme linked immunosorbent assay elisa against human ige bound to an immulon 2 plate one of these hybridomas produced tes c21 sp 2 0 cells were co transfected with the variable regions of tes c21 h and l chains and human u03b31 and u03ba constant regions and aliquoted into 96 well plates for selection supernatants were screened for secretion of human igg which bound to human ige the transfectoma cells were adapted to growth in serum free medium tesc 2 was then purified from medium of confluent cultures using an immobilized protein a column tes c21 was further screened by elisa to be specific for human ige and not to react with igg igm iga igd human serum albumin transferrin or insulin tes c21 bound equally well to various human ige molecules tes c21 bound to the ige secreting cell lines sko 007 u266 and se44 in a dose dependent manner indicating binding to human membrane.