by the results shown in table iii aqueous isopropanol effectively destroys coliform organisms table iii______________________________________ number of coli coliforms forms surviving surviving exposure mpn mpn source of time isopropanol isopropanolbarley malt minutes 25 50 75 25 50 75______________________________________brand a 15 6 7 1 3 11 8 0 6 0 1 1 1brewer 39 s malt 30 12 9 0 2 1 6 1 2 0 2 0 2 60 0 5 0 0 6 0 1 0 0 1brand b 15 19 7 4 6 3 3 1 1 0 3 0 2distiller 39 s 30 12 3 5 9 0 7 0 7 0 7 0 3malt 60 2 1 3 9 2 2 0 1 0 2 0 1brand b 15 9 9 24 0 2 0 0 8 1 8 0 2brewer 39 s malt 30 7 9 6 8 5 3 0 6 0 5 0 4 60 1 2 0 6 0 3 0 1 0 1 0 2______________________________________ most probable number additional studies were conducted to determine the effect of the aqueous alcohol upon the alpha and beta amylase activity in particular barley malt was treated with an aqueous solution containing 70 isopropanol by volume at room temperature for both 1 and 24 hours the malt was rinsed thoroughly and dried at low temperatures which did not exceed 140 u00b0 f diastatic power determinations employing aacc method 22 10 and using 1 gram of malt demonstrated no significant loss of enzymatic activity in particular barley malt which had been subjected to the aforementioned concentrated isopropanol solution for 1 hour demonstrated a diastatic activity of 390 brabender units while the barley malt treated under similar circumstances for 24 hours was determined to have 410.