2 tumors and pnc prevalence below the median 37 74 had a higher survival rate than patients with tumors of higher pnc prevalence similarly patients with grade 3 tumors and pnc prevalence below the median 49 87 had a higher survival rate than patients with tumors of higher pnc prevalence moreover patents with a pnc prevalence rate above 37 74 had a lower overall survival rate than patients with a pnc prevalence rate of 28 35 fig6 and 7 these findings together show that the formation of the pnc correlates with the progression of the malignancy and high pnc prevalence in primary tumor is indicative of poor survival of patients these findings are consistent with the observations in breast cancer studies that higher pnc prevalence in primary tumor associates with advance stage of the malignancy pnc prevalence is correlated with the progression of prostate and colon cancer pnc prevalence is also found to be positively correlated with disease progression in prostate and colon cancers comparing the staining pattern of ptb in normal prostate samples and in malignant prostate samples fig9 shows significantly higher prevalence of pnc in the malignant samples compared to the normal samples similarly observations were made for colon cancer as well data not shown to examine the relationship between.