24 ms have been reported by the device this approach produced hundreds of zeocine resistant transformants per microgram of transformation construct used fig1 shows the sequence of an exemplary transformation construct the exemplary transformation construct 100 includes a promoter sequence 102 dna encoding a transcribed but untranslated 5 u2032 region u201c utr u201d 104 an intron 106 a ble gene 108 and dna encoding a 3 u2032 utr 110 the transformation construct may comprise any number of promoters genes and or other nucleic acid polymers naturally occurring or synthetic and or their analogs or other compounds that do not interfere with the ability of the transformation construct to enter the algal cell or the algal genome or to function in some embodiments additional nucleotides may appear in the transformation construct to facilitate or direct the insertion of the construct or any part thereof into a desired location in the genome the various exemplary methods described herein may be employed using the exemplary transformation vectors or transformation constructs pl90 h8 and b9 as described in u s non provisional patent application ser no ______ filed on jun 8 2009 titled u201c vcp based vectors for algal cell transformation u201d which is hereby incorporated by reference the difference between the three exemplary vector constructs is the type of selection marker gene sg used the sh ble gene pl90 the hygromycin b phosphotransferase gene h8 or the blastocidin s deaminase b9 gene fig2 illustrates an exemplary method for transforming an algal cell with a particle gun at step 205 a desired transformation construct is prepared according to one exemplary embodiment the transformation construct may be either the pl90 transformation vector the.