ischemia where the perfusate flow is completely shut off followed by 60 minutes of reperfusion hearts are treated with 10 u03bcm compounds of the formula ii wherein n is 1 or 2 and the chirality at the carbon atom is rs u00b1 r or s u2212 in the present examples compounds of the formula ii treated diabetic group dz hearts are subjected to 10 minutes of normoxic perfusion with normal krebs henseleit buffer and 10 minutes of normoxic perfusion with krebs henseleit buffer containing 10 u03bcm present compounds of formula ii the hearts are then subjected to 20 minutes of zero flow ischemia followed by 60 minutes of reperfusion in order to avoid any variability in reperfusion conditions both dc and dz hearts are reperfused with normal krebs henseleit buffer this example describes a procedure used for study of low flow ischemia in diabetic controls diabetic treated non diabetic treated and non diabetic control isolated hearts diabetic control hearts dc are subjected to 20 minutes of normoxic perfusion at a flow rate of 12 5 ml minute followed by 30 minutes of low flow ischemia where the perfusate flow is slowed down to 1 25 ml min that is about 10 of normal perfusion followed by 30 minutes of reperfusion at a normal flow rate 12 5 ml min in the compounds of the formula ii wherein n is 1 or 2 and the chirality at the carbon atom is rs u00b1 r or s u2212 treated diabetic or non diabetic groups dz or cz hearts are subjected to.