point the plates can be sealed and stored at 4 u00b0 c for up to one two months single stranded analyte dna is now fixed to the wells an alternative method to denature and then fix the analyte dna to the well is to add 50 ul of dna in te to wells at a concentration of 10 u2013 200 ng 50 ul after adding 25 ul at 0 9 n naoh and mixing the plates can be incubated for 10 minutes at room temperature after adding 25ul of 4 m nh 4 oac the open plate may be incubated at 37 u00b0 c for 4 hours or until dry and the plates sealed and stored at 4 u00b0 c until ready to use to prepare the plates for hybridization the wells would be rinsed twice with 0 3 m nacl 0 03 m sodium citrate 2x ssc 200 ul well buffer regardless of whether the plate was dried or not preferably the wells can be rinsed once with 2x ssc 1 triton x 100 after the two 2x ssc rinses plates should be blotted on absorbent paper before beginning each rinse to hybridize the fixed analyte with a probe the following protocol would be followed a nick translated probe would be heat denatured and added to a hybridization solution containing 30 formamide deionized 2x u2013 4x sspe 20x sspe 3 6 m nacl 0 2 m napo 4 ph 7 4 0 02 m edta depending on the gc content of probe 0 1 sds.