in a concentration of 40 mg ml by dry weight in 1 30 m phosphate buffer solution ph 8 0 in this step 200 mg of u00b1 cis 7 u03b2 phenylacetamido 4 u03b1 hydroxy 1 azabicyclo 4 2 0 oct 2 en 8 on 2 carboxylic acid obtained as in reference example 2 below is added into 9 ml of 1 30 m phosphate buffer ph 8 0 since the compound is not dissolved 2 n naoh is added in a small portion and the mixture is again adjusted at a ph of 8 0 to dissolve the compound finally deionized water is added to make 10 ml of a solution in this step 10 ml of the disrupted cell suspension mentioned above is added in 10 ml of a substrate solution and enzyme reaction is carried out at a temperature of 40 u00b0 c for 80 minutes time course of the reaction is illustrated in table 1 table 1______________________________________ the amount ofreaction period compound i 1 yield minutes produced mg ml mol ratio ______________________________________10 0 8 12 520 1 3 2040 1 8 2960 2 1 3380 2 2 35______________________________________ compound i1 wherein r sub 1 is h and r.