with 0 7 dnfb solution and boosted with 0 6 dnfb solution lldt 8 was tested to confirm whether it has immunosuppressive effect on cell mediated immune responses on the classical animal model of dnfb induced mouse delayed type hypersensitivity response dth the results indicated that lldt 8 showed a very potent inhibitory effect on dth when i p or p o administered to mice lldt 8 significantly suppressed dnfb induced mouse dth when intraperitoneally administered to mice at the dose of 1 mg kg d for 5 days the potency of inhibitory effect was even higher than that of cyclosporin a 10 mg kg there was no obvious influence on lymph organs spleen and body weight in dth mice when treated with lldt 8 at the dose of 1 mg kg d table 5 lldt 8 treatment with medication change in type the dosage and frequency 1 mg kg 0 2 mg kg and 0 04 mg kg once a day for 2 days still exerted suppressive effects to the same level as that of csa 10 mg kg table 6 lower concentration of lldt 8 at the dose of 0 4 u03bcg kg still markedly inhibits the dth response to the same level as those of ctx at 1 mg kg and lldt 2 at 40 u03bcg kg table 8 the.