curves in combination a template curve with the least statistical error or a combination of the template curves that give the linearly bound curve with the least statistical error should be defined as the sequence characteristics of a single stranded dna fragment prepared from the sample double stranded dna fragment fig9 depicts the derivative of the melting curve of a heterozygote sample of 0102 and 0301 representing dqa1 0102 and dqa1 0301 together with the characteristic parameters of the curve in such heterozygote sample the derivative is represented as the superposition of template curves of the individual dna type which indicates that typing can be carried out on the principle of spectral analysis on the basis of the results of fig9 table 2 summarizes the parameters of the individual dna types more specifically if a heterozygote dna has the parameter values of u03bc peak location and u03c3 standard deviation being almost the same as those represented as the regression values shown in table 2 the heterozygote dna is of a heterozygote of two dna types deduced from the values table 2______________________________________ regression regression u03bc values u03c3 values dna types______________________________________ u03bc1 16 9 u03c31 6 89 0301 u03bc2 19 0 u03c32 11 0 0102 u03bc3 28 9 u03c33 11 5 0301 u03bc4 35 9 u03c34 9 0 0102______________________________________ in the present example a satisfactorily accurate melting curve can be generated in a practical sense at a temperature elevation rate of 5 u00b0 c min at maximum in this case the analysis time is about 10 minutes per.