curve 25 illustrates the released fluorescence vs time of fluorescein tagged molecules of human serum albumin hsa which have been digested by the proteolytic enzyme bromelin point 34 a 34 depicts the initial level of fluorescence prior to the digestion of the hsa molecule about 25 of the maximum expected level of fluorescence for a free amount of unbound fluorescein as the hsa molecule is digested by the bromelin an increasing amount of fluorophores are released and the fluorescent level is increased as the hsa molecule is completely digested by the bromelin i e after a reaction time of approximately 150 minutes almost all of the fluorophores will be released to give a fluorescent level approaching 90 of maximum as shown by point 34 b 34 fig6 therefore shows that a carrier molecule 11 such as fluorescein labeled hsa can function to enhance a fluroroimmunoassay of an antigen or antibody according to the outlined method where the molecule can be lysed by more than one enzyme several enzymes may be simultaneously used to break up the carrier molecule into finer segments this will give rise to a greater fluorescence for example in a protein molecule carrier trypsin will cleave the protein.