tmac eight different serial dilutions are prepared from the stock target solution 20 u03bcl of each of the serially diluted target solutions are then added to the eight separate chips 2 a slide containing the chips is placed in a hybridization heater shaker and incubated at 55 u00b0 c for 20 minutes at 100 rpm 3 the slide is removed and cooled to room temperature and the hybridization solution is removed with the transfer pipette 4 20 u03bcl of 1 u00d7 tmac is added to each chip and the chip is washed by pipetting the solution 8 to 10 times 5 the washing solution is removed and 5 ml of mounting solution 1 u00d7 tmac is added to each chip and the assay signal cy5 is read under a fluorescent microscope using a coverslip 6 a titration curve is plotted of the hybridization signal cy5 vs dna probe concentration example of titration curves are shown in fig3 experiments were conducted to compare the effect of adding edac to the bead probe suspension twice edac is known to hydrolyze very quickly at acidic ph to assess whether this leads to an enhanced binding of probes to the bsa layer first the probe ms 508 n25 was coupled to bsa coated beads under each of the following condition 10 u03bcl 100 u03bcm probe 100 u03bcl 1 beads one half of the beads were removed from the.