0 001 dc antifoam a 0 05 ph 7 1______________________________________ after the corn steep liquor was mixed with the beet molasses and the ph was adjusted to 7 1 the sludge of the corn steep liquor was removed by filtration the fermenter was sterilized for 60 minutes at 121 u00b0 c fermentation was started by adding 10 seed culture into the medium in the fermenter aeration and agitation were set at 4 liter per minute or 0 4 vvm and 300 rpm respectively temperature was set at 33 u00b0 c the enzyme activity was determined according to the methods described in example no 1 table vi shows the results concerning the yields of cells and enzyme from bm sf medium and bm csl medium the yield of enzyme is greater using bm sf medium as compared to a bm csl medium the medium of this invention is preferred over a corn steep liquor medium for actinoplanes missouriensis because the medium does not have to be filtered to remove sludge as does a corn steep liquor medium table vi__________________________________________________________________________comparative study of enzyme yield in fermenter using beet molasses cornsteep liquor medium andbeet molasses soy flour medium as a fermentation medium __________________________________________________________________________ beet molasses corn steep liquor medium beet molasses soy flour medium solid enzyme solid enzyme harvested yields harvested sp act yieldsfermentation g dry wt sp act u ml g dry wt u g dry u ml periods hr 100 ml cult u g dry solid culture 100 ml cult solid culture __________________________________________________________________________24 0 63 3062 19 6 0 91 2665 24 648 0 94 3718 35 0 1 23 4400 54 060 0 90 4550 41 0 1 28.