or with branched chains cyclohexylamine bensylamine ethanolamine propanolamine aniline substituted anilines allylamine hydroxyamine etheramine as alcoxy propylamine secondary amines are for example dimethylamine diethylamine and so on with straight or branched chains being symmetric or asymmetric morpholines piperidines diethanolamine dicocoamine secondary etheramines or fatty acid alkylamines diamines are for example hydrazine ethylenediamines laurylamineethylamine piperazines triamines are diethylenetriamine or polyamines analysis method the sample is treated with bis trimethylsilyl trifluoroacetamide and chlorotrimethyl silane in methylene chloride in a tube with a tight fitting at 70 u00b0 c for 30 minutes the sample is then analyzed by gas chromatography in a capillary column se 30 25 m long phase thickness 0 25 u03bcm id 0 32 mm cholesterol 99 5 merck was used as internal standard water was removed from 1 kg soybean desodorisate by simple distillation 400 g of the residue were treated with 120 g of laurylamine dodecylamine at 200 u00b0 c for 18 hrs the reaction was chosen with respect to the completeness of the liberation of sterols where also the sensitivity to temperature of the sterol must be taken in consideration after purification we isolated 27 g of a mixture of sterols mainly containing beta sotosterol campesterol stigmasterol total contents of sterols in the product was about 90 400 g of pitch from tallow distillation were treated with 33 g of hydrazine hydrate in a three necked flask provided with a stirrer at 108 u00b0 c for 22 hrs after which time period about 75 of the sterols present were free after purification 18 g of a mixture of mainly cholesterol sitosterol campesterol and stigmasterol were obtained the total content of sterols in the product was about.