clontech ca usa were used in these plasmids gene expression is under the control of cytomegalovirus cmv promoter the plasmid dna was amplified in e coli xl1 blue stratgene tx usa and the amplified plasmid dna was used as a complex partner the dna was dissolved in distilled water free from dnase and rnase the following transfection reagents were used effectene transfection reagent cat no 301425 qiagen ca transfast u2122 transfection reagent e2431 promega wi tfx u2122 20 reagent e2391 promega wi superfect transfection reagent 301305 qiagen ca polyfect transfection reagent 301105 qiagen ca lipofectamine 2000 reagent 11668 019 invitrogen corporation ca jetpei u00d7 4 conc 101 30 polyplus transfection france and exgen 500 r0511 fermentas inc md lipofectamine 2000 is preferably used but is not limited thereto these transfection reagents were added to the above described dna and actin acting substance in advance or complexes thereof with the dna were produced in advance the thus obtained solution was used in an improvement test for transfection efficiency as described below in the present example an improvement in the transfection efficiency in solid phase was observed the protocol used in the present example was followed according to the manufacturer 39 s instructions for lipofectamine 2000 in the present example the effects of these substances in liquid transfection using pc12 cells were investigated in the presence or absence of the cellular adhesion related agents as prepared above pc12 rat pheochromocytoma cells atcc crl 1721 were cultured in dmem 10 calf serum gibco supplemented with l glutamine and penicillin streptomycin cellular adhesion related agents were stored as a stock of 200 u03bcg ml in pbs all dilutions were made using pbs ddh 2.