with pbs solution alternatively glass coverslips or plate wells can be pre coated with the nanoparticles allowing cells to be seeded on top of the nanoparticle layer wells of the plate will be filled with nanoparticle containing solution at the appropriate concentration the plate can be stored for several hours under the sterile conditions after the nanoparticles containing solution is washed away the coverslip will be transferred into a special microscope chamber 508sw ala scientific instruments westbury n y and maintained in buffered ebss solution during the experiment glass micropipettes for patch clamp experiments will be pulled from horosilicate glass capillaries sutter 1 2 mm no capillary glass using a sutter 2000 u2122 pipette puller model sutter 2000 sutter instruments novato calif using a prerecorded 4 step patch pipette pulling protocol the open diameter of the pipette tip will be 1 5 2 2 u03bcm the micropipettes will be filled with a solution containing 140 mm potassium aspartate 5 rum nacl and 10 mm hepes ph 7 35 voltages and currents will be recorded at room temperature using a axopatch 200b patch clamp amplifier molecular devices sunnyvale calif after establishing the successful giga seal brief pulses of suction will be used to.