in pbs for 1 hour at room temperature after incubation with appropriate secondary antibodies blots were incubated in enhanced chemiluminescence reagents amersham corp and exposed to photographic film all data are represented as mean u00b1 standard error se of the mean a two tailed paired student 39 s t test was used to compare same animal data while all other data were grouped according to treatment and analyzed using an unpaired student 39 s t test p values lt 0 05 were considered statistically significant for all comparisons 0055 fig2 shows representative results of northern blot analysis of inos and ho 1 mrna expression 24 hours following left ca balloon injury the lanes represent the ipsilateral injured left ca l the contralateral uninjured right ca r and ca from control c rats which were not subjected to balloon injury gapdh is included as a loading control between 8 and 10 animals were used per lane to obtain sufficient amounts of rna northern blot analyses demonstrate upregulation of both inos and ho 1 mrna in the ipsilateral left ca l 24 hours following balloon injury the contralateral right ca r also demonstrates induced ho 1 mrna at this time point however no detectable inos signal is evident in.