provided to further illustrate the advantages and features of the present invention but are not intended to limit the scope of the invention while they are typical of those that might be used other procedures methodologies or techniques known to those skilled in the art may alternatively be used this example illustrates how the present method can be used to generate a mutant adaptively evolved strain that synthesizes larger quantities of an antibiotic than the wild type strain in this case the producer organism was streptomyces clavuligerus atcc 27064 and the target organism was methicillin resistant staphylococcus aureus strain n315 mrsa n315 mrsa n315 is resistant to erythromycin and many u03b2 lactam antibiotics such as penicillin wild type s clavuligerus mycelia or spores are first inoculated into a triple baffled flask containing trypticase soy broth tsb and incubated at 28 30 u00b0 c a magnetic stir plate spun a stir bar inside the flask at approximately 1500 2000 rpm to aerate the liquid broth once the culture reached an optical density at 600 nanometers 0d600 between 0 08 and 0 13 2 u03bcl aliquots of the culture were spotted onto each of seven equidistant points on a trypticase soy agar tsa plate if the culture was denser than 0 13 it was diluted with tsb until the measured od600 value fell between 0 08 and 0 12 the use of an od600 culture between 0 08 and 0 13 the use of a 2 u03bcl dispensing volume and the choice of seven spots per plate versus.