serums were tested for igg induction against the immunogen mimotope peptide as well as the carrier klh alone by the elisa assay as already shown with mice igg directed specifically against mimotopes could also be achieved in rabbits this is shown by the following example her 2 neu is recognized in the cytomembranes of the cell line skbr3 in elisa by the humanized monoclonal antibody herceptin for the elisa inhibition assay one tries to prevent this bond by preincubation with mimotopes on phages or klh as carrier the wells of a 96 well elisa plate nunc were coated with membrane fractions of the her 2 neu overexpressing cell line skbr3 and as a control for non specific antibody bonds with membrane fractions of the her 2 neu negative cell line htb 132 in pbs overnight herceptin anti her 2 neu antibody was treated likewise overnight by preincubation with different antigens mimotopes control mimotopes or buffers the next day non specific binding sites were blocked by 3 percent bsa in pbs herceptin as well as herceptin after preincubation with relevant and irrelevant phage mimotopes or by mimotopes on klh as well as on klh alone were applied to the coated blocked elisa plate in a repeated test bound herceptin could be detected with a horseradish peroxidase hrp coupled monoclonal anti human igg hrp enters into a.