of the wound in addition to preventing infection in the area the sequence for the c myc gene has been determined and is available under accession l00038 from embl in a further embodiment specifically directed at improved wound healing or stimulation of cell growth the cassette comprises the c myc promoter linked to vishnu 1 or a lytic peptide analogue having similar properties vishnu 1 is a truncated version of shiva 1 which lacks the cytolytic and toxic properties of shiva 1 but still induces proliferation of mammalian and bacterial cells a pvui restriction enzyme digest of pilshi neo was performed to linearize the dna and remove a portion of the vector not required for mammalian cell expression the fragment was purified by agarose gel electrophoresis and separated from the gel slice using geneclean bio 101 la jolla calif 92038 2284 the dna was eluted from the glass beads using 10 u03bcl of injection buffer 10 mm tris 0 15 mm edta ph 7 4 the solution was then dialyzed through a 0 025 u03bcm pore size mixed esters of cellulose filter membrane millipore bedford mass 91013 against three changes of a 10 ml pool of injection buffer over 72 h the dna concentration of the recovered solution was determined by staining with ethidium bromide and comparing against standards then the concentration of the solution was adjusted to 2 5 ng u03bcl twenty five six week old female mice of strain b6sjlf1 j jackson laboratory were used as embryo donors twelve hours before the midpoint of the daily dark cycle they were given intraperitoneal ip injections of seven iu international units of pregnant mare serum gonadotropin pmsg forty seven.