cctccm200032 the depositor names it escherichia coli jm109 jh 5 pns tpa according to the above methods a novel cell line with the desired gene tnk tpa integrated in the short arms of human group d or group g chromosomes of an e coli ht1080 cell the cell line was accepted by china typical culture collection center wuhan university china wuhan 430072 on aug 18 2000 the collection number is cctcc c200006 the cell line is a human fibrosarcoma cell line which is human gene transformed cell the depositor names it human fibrosarcoma cell line jh 2 tpa the preserved cell line is a pure cultivative cell line the cells may be cultured in 10 calf serum in dmem ph 7 0 7 4 at 37 u00b0 c the above cell line can substitute for transformed cho transformed cells to prepare tnk tpa the purified protein expressed by the cell line of the invention has been confirmed to be the desired protein by western blotting and amino acid sequence analysis the invention provides a novel cell line by site directedly transferring a recombinant human source gene vector tnk tpa cdna into d group and or g group chromosomes of a human ht1080 cell and so provides a method to produce tnk tpa compared with the existing technology the invention dramatically decreases cost of tnk tpa because of its efficient and stable expression fig1 shows fish mapping of 120 kb bmsf fragment cloned in pac fig2.