were blotted onto the membrane to identify the gene encoding l41 protein without intron phaffia rhodozyma l41 cdna was isolated by the method of rapid amplification of cdna ends race with 3 u2032 race gibco brl usa and 5 u2032 race clontech usa kits total rna was prepared by the method of elion and warner elion et al cell 39 663 u2013 673 1984 then mrna was selected from the total rna using mrna isolation kit novagen and a 3 u2032 race reaction was performed with a synthetic oligonucleotide represented by seq id no 7 as a 3 u2032 race primer and 5 u2032 race reaction was ferformed with a synthetic oligonucleotide represented by seq id no 8 as a 5 u2032 race primer the sequencing of the 3 u2032 and 5 u2032 race products suggested that a putative open reading frame of 1 223 bp be interrupted by six introns the cloned gene encoding l41 protein was found to show high homology with those of other yeasts however the number of introns and their organization in the gene encoding the l41 protein of phaffia rhodozyma were quite different from those of the other yeast in fact they have only one intron gtpungt sequence and pyag sequence were conserved in 5 u2032 and 31 ends respectively of the gene encoding l41 protein of phaffia rhodozyma this conserved sequences were also reported in the intron of actin of phaffia rhodozyma the l41 gene encodes ribosomal protein comprising 106 amino acids and most notably proline at position 56 is identified to the amino acid residue responsible.