centrifugation and the insoluble fraction is treated with detergent the supernatant after centrifugation was either subjected to aerosil adsorption and desorption traditional technology table 1 or to primary capturing of hbsag by a batch procedure in which salts of divalent cations such as calcium magnesium and zinc are added at 0 2 to 10 w v in the presence of phosphates chlorides or acetates to form white insoluble matrix the insitu formation of the matrix further interact with the antigen and this process of protein capturing is referred as himax technology table 20 this matrix was separated by centrifugation between 7000 g to 10 000 g and bound antigen was desorbed repeatedly with this buffer of ph 8 5 the desorbate was further purified using an anion exchange matrix namely the deae the hbsag activity in all the intermediate steps is given in table i and table ii in another strategy the cell lysate is directly subjected to primary capturing of the antigen by cations at 0 2 to 10 in the presence of phosphates chlorides and acetates all subsequent steps are similar to earlier procedure the hbs ag activity in all the intermediate steps is given in table iii the major difference between table 2 and table 3 is the usage of detergent in the table 2 the insoluble fraction is treated with detergent and further processing is carried with adsorption and desorption technology while in the experiments.