mitotic cell cycle progression the condensin complex involved in chromosome condensation was shown to display high affinity for structured dna such as cruciform dna kimura and hirano cell 1997 90 625 634 to examine the dna binding activity of braf35 a comparison of the dna binding activity of braf35 to that of the baf57 hmg domain was made wang et al proc natl acad sci 1998 usa 95 492 498 recombinant braf35 displayed strong binding to 4wj dna in gel shift assays however in contrast to two other sequence specific hmg domain containing proteins lef1 and mtf1 braf35 did not display detectable dna binding activity when either of the duplex u201c arms u201d of the 4wj dna were used as probes these results demonstrate that the dna binding properties of braf35 are highly specific and similar to that of the condensin complex kimura and hirano cell 1997 90 625 634 microinjection of anti brca2 or anti braf35 antibodies into synchronized hela cells causes a g2 cell cycle delay to functionally assess the role of the brca2 braf35 complex in cell cycle progression synchronized hela cell nuclei double thymidine block were injected with either affinity purified anti braf35 or anti brca2 antibodies and the injected cells were analyzed 12 and 14 hours following release from the block cells were stained to identify the injected antibodies and counterstained with anti cenp f liao et al j cell biol 1995 130 507 518 cenp.