ml of 1 5 peracetic acid sigma solution with 1 5 triton x 100 sigma in distilled deionized water dih2o was added and the sample placed onto the rotary shaker at 200 rpm 37 u00b0 c for 4 hours the solution was discarded and two 100 ml washes with dih2o were performed each for 12 hours at 37 u00b0 c and 200 rpm on the rotary shaker at the end of the second wash the sample was removed and placed into a clean sterile 15 ml conical tube and frozen for 24 hours at u2212 80 u00b0 c the sample was then freeze dried labconco freeze dry system kansas city mo for 24 hours before being returned to the freezer and stored at u2212 80 u00b0 c until further use b histologic analysis of fresh frozen fdp tendons and decellularized oxidized fdp tendon scaffolds mid substance portions of fresh frozen fdp tendon and decellularized oxidized fdp tendon scaffold were placed in 10 phosphate buffered formalin at room temperature for 4 hours the tendons then were processed for histology embedded in paraffin and microtomed to obtain 5 0 u03bcm thick longitudinal sections the sections were mounted on slides and stained using hematoxylin and eosin h amp e sigma as well as 4 6 diamidino 2 phenylindole dapi vector burlingame calif to identify cellular and nuclear components respectively representative light h amp.