was prepared using the same ingredients and procedure as described in example 1 except that the amount of tetracycline used was 1 0 gram and the amount of sugar used was 9 0 grams the concentration of tetracycline in the final composition was 500 ppm a control composition was prepared using the same ingredients as stated in example 1 except that no tetracycline was included the diet compositions described in examples 1 3 were fed separately on a no choice basis to separate groups of laboratory mice swiss webster infected with the lyme disease spirochete borrelia burgdorferi eleven mice ten study and one control were used for each test composition the mice chosen had been previously infected with b burgdorferi by subcutaneous injection followed by a 21 day incubation period to confirm the mice were positive for the spirochete a modified ear punch biopsy was performed sinsky and piesman 1989 j of clin micro 27 1723 1727 cdca 1997 on each mouse and the biopsy incubated in modified bsk h media cdcb for 7 days and viewed under dark field microscopy the prepared diets were fed to individually housed mice on a no choice basis and water was offered ad libitum the control mice were offered an identical diet minus the active ingredient consumption was recorded each day and additional feed was added diet was presented to the mice for seven consecutive days on the day following the last day feed was offered mice were anesthetized with methoxyflurane and ear punch biopsy was performed the biopsies were incubated in modified bsk h media for seven days at 34 u00b1 1 u00b0 c dark field microscopy was then performed on an american optics 1031 microscope with an american.