pasteurized microfluidized nanoemulsion is presented in table 13 the original microfluidized nanoemulsion was freeze thaw tested at u2212 4 u00b0 c for 24 hours twenty four hours later the oil was still in solution and the particle diameter was stable as compared to the original nanoemulsion see fig1 the average particle diameter data for the freeze thaw microfluidized nanoemulsion is presented in table 14 the microfluidized nanoemulsion was incorporated into a chow based diet and fed to gerbils over a 4 week period a control group was fed a lycopene in oil enriched chow based diet at the end of 4 weeks blood was collected plasma harvested and plasma lycopene levels were determined by hplc in both gerbil groups fig1 demonstrates that control gerbils did not demonstrate detectable plasma lycopene levels the gerbils fed a chow comprising a microfluidized lycopene nanoemulsion however demonstrated elevated plasma lycopene levels see fig1 a microfluidized lycopene nanoemulsion was then prepared with grape juice instead of water and orally administered to two 2 human subjects over a 4 day period 125 mg serving 2 servings per day this administration raised plasma lycopene levels by approximately 38 data not shown this example presenting data showing that microfluidized nanoemulsions provide improved efficacy over that seen in traditional nanoemulsions specifically this example compares the ability of three plant sterol formulations to reduce plasma low density lipoprotein cholesterol ldl c levels in hypercholesterolemic hamsters a microfluidized mixed plant sterol 60 sitosterol nanoemulsion was prepared.