the solution was carefully adjusted to 5 9 u00b1 0 1 the hplc analysis of the product of the above process taken after storage of the reaction solution at ph 6 0 for 21 hours is presented in fig3 the presence of only a single peak relating to ring opened saccharide products namely the hexa aldehyde o raffinose is noteworthy and indicates homogeneity of the product and absence of alkaline hydrolysis products the only other significant peaks on the chromatogram pertain to salts and formic acid which are readily removable the o raffinose may be purified further by desalting on using a ion exchange mixed bed resins b size exclusion chromatography or c reverse osmosis a solution of o raffinose 20 ml ph 5 65 was adjusted to ph 1 6 by dropwise addition of 6 n hcl and then the solution was passed through 30 ml of biorad ag 501 8 d analytical grade mixed bed resin the eluent was collected and purified sample lyophilized to provide a solid white crystalline product modified method for controlled cross linking of hemoglobin by o raffinose followed by reduction with dimethylamine borane a 30 ml solution of purified human hemoglobin 8 w v in 50 mm bis tris buffer at ph 6 5 was.