34 an alkalophilic variant of bacillus licheniformis all commercially available from novo nordisk industries a s 34 mexatase 34 and 34 maxacal 34 from gift brocades 34 kuzusase 34 of showa denko and 34 bpn 34 protease from bacillus subtilus made by genincor or sigma the activity of the proteolytic enzyme included in the composition typically ranges from about 0 1 150 au g or its equivalent mixtures of different proteolytic enzymes may also be used standard measures of enzyme activity include the anson unit au the kilo novo protease unit knpu and the glycine unit gu these measures of enzyme activity are well known and defined as follows one anson unit is the amount of enzyme that digests hemoglobin at an initial rate such that there is liberated per minute an amount of tca soluble product which gives the same color with phenol reagent as one milliequivalent of tyrosine the reaction conditions for this measure are given in nias method af4 5 gb modified anson hemoglobin method for the determination of proteolytic activity one glycine unit is the amount of enzyme that produces the equivalent of one micromole of glycine per minute under assay conditions one kilo novo protease unit knpu is the amount of enzyme that hydrolyses casein at such a rate that the initial rate of formation of peptide minute corresponds to i mole of glycine minute the standard conditions for carrying out this test are given in nias method af 162 3 6b manual dmc dimethyl casein method for the determination of proteolytic activity the proteolytic enzyme of the invention should have an activity of 12 knpu gram plus or minus about 20 sequestering agents are added to the composition to maintain clarity and to promote calculus or.