initial concentration of atp is very low in one example the concentration of atp is amplified more than 5 fold by the three pathway system when compared to that of the pathway for luminescence only furthermore when the regenerated atp obtained from either the pathway for ppi reaction 112 or the pathway for amp reaction 114 repeats the pathway for luminescence 110 more light is produced and detectable therefore detection sensitivity can be increased without altering conventional luminometers except for luminescence detection one skilled in the art can appropriately design the three pathway system for relevant clinical or industrial uses in addition conventional luminescence detection uses atp in microbial cells as an initial substrate to detect the luminescence from the conversion of atp to light when the amount of microorganisms in a sample is minor the luminescence is relatively low as such the impurity in the sample easily leads to background noises and the sensitivity of detection is insufficient therefore the sensitivity of conventional luminescence detection is only up to 10 4 cfu ml of microorganisms the method for amplifying atp according to the application uses the three pathway system to amplify the amount of atp and produce more light for detection therefore the detection for the concentration of microorganisms by using the method of the application can effectively lower interference from background noises and the sensitivity can reach to less than 10 4 cfu ml of microorganisms the sensitivity and stability of the detection can be thereby increased in one example the sensitivity by using the method of the application is approximately 10 2 cfu ml of microorganisms which is nearly the detection limit of conventional luminometers therefore even if.