or isotype control antibody known not to bind mb 231 cells was administered intrapertioneally at a volume of 300 u03bcl after dilution from the stock concentration with a diluent that contained 2 7 mm kcl 1 mm kh 2 po 4 137 mm nacl and 20 mm na 2 hpo 4 the antibodies were then administered once per week for a period of 7 weeks in the same fashion tumor growth was measured roughly every 7th day with calipers for up to 10 weeks or until individual animals reached the canadian council for animal care ccac end points or day 120 body weights of the animals were recorded for the duration of the study at the end of the study all animals were euthanised according to ccac guidelines the data presented in this study is a typical example of a longitudinal data set usually in such data sets there are high correlations among time points and higher correlations are observed between closer time points because of this repeated measures analysis of variance rep anova was used to determine the differences among treatments and the method of analysis of covariance was used to determine the time points when differences occurred the latter is a suitable method when the differences among groups at each time point may not be just due to groups but may be due to the previous time points.