is performed in a final volume of 50 u03bcl containing 2 5 mm mgcl 2 75 mm tris hcl ph 9 0 50 mm kcl 20 mm nh 4 2 so 4 0 5 u03bcm of the 0 1 u03bcm of the primers 200 u03bcm of datp 200 u03bcm of dctp 200 u03bcm of dgtp 150 u03bcm of dttp 50 u03bcm of biotin 16 dutp 0 5 u of uracil dna glycosylase 1 25 u of taq dna polymerase 5 ng of plasmid containingfema gene the reactions are first denatured at 94 u00b0 c for 5 min and then cycled 40 times in a dna 9600 thermocycler using the following temperatures and cycle times 94 u00b0 c for 30 s 49 u00b0 c for 45 s 72 u00b0 c for 30 s a final extension step of 10 min at 72 u00b0 c is performed pcr products are directly used or frozen water controls are used as negative controls of the amplification the hybridization protocol was described in example 5 slides are incubated 45 min at room temperature with 800 u03bcl of streptavidin labeled with colloidal gold 1000 u00d7 diluted in buffer 2 after incubation slides are washed 5 times 1 min with buffer 1 then rinsed once with water gold catalyses silver precipitation using silver blue revelation solution aat namur belgium the slides are incubated 3 times 10 min with 800 u03bcl of revelation mixture then rinsed with water dried and analyzed using a microarray reader each spot are then quantified by a home made quantification software the results gave a hybridization value of 181 for the diaglass slides and 111 for the telechem detection of short target nucleotides on complementary capture nucleotide sequences fixed on glass slides.