in a soxhlet extractor 10 g of the defatted brine water washed residue was extracted in a soxhlet extractor with 200 ml methanol in a soxhlet extractor with 70 ml capacity up to siphoning level of the extractor the extraction continued for 16 hours the solvent was removed from the extract by distillation and finally in a rotary evaporator and dried at 60 u00b0 c for 2 hours cooled in desiccator and weighed the weight was 1 0 g about 80 of this extract could be redissolved in 100 ml of ethanol to have a stock solution of the antioxidant hplc analysis of antioxidant extract by reverse phase and quantification of separated peaks was performed as follows in an actual experiment a shimadzu make lc 10 a d analytical hplc equipped with a rheodyne injector with 20 u03bcl sample loop a uv visible detector and c r7ae model data analyser was used the column connected was u03bc bondapak column 4 6 mm u00d7 25 cm and solvent system tried was methanol water 70 30 the uv detector was set at 290 nm fig8 represents the hplc chromatogram quantitation of separated peaks was done by calibrating with standard sesamol sigma aldrich co usa the peaks.