control metabolite can be obtained in step b each ratio of the one or more than one metabolite marker to the internal control metabolite could then be compared to corresponding data obtained from one or more than one reference sample in step c the present invention also provides high throughput methods for diagnosis of prostate cancer the method may involve fragmentation of the parent molecule in a non limiting example this may be accomplished by a q trap u2122 system detection of the metabolites may be performed using one of various assay platforms including colorimetric chemical assays uv or other wavelength antibody based enzyme linked immunosorbant assays elisas chip based and polymerase chain reaction for nucleic acid detection assays bead based nucleic acid detection methods dipstick chemical assays or other chemical reaction image analysis such as magnetic resonance imaging mri positron emission tomography pet scan computerized tomography ct scan nuclear magnetic resonance nmr and various mass spectrometry based systems the hts method may involve the measurement of intensity peak area or summed scans of each selected daughter ion per biomarker as well as daughter ions for one or more internal standards added to each sample a ratio may then be generated by dividing each biomarker transition.