6 3 4 dichloro phenyl carbamic acid 4 oxo 4 phenyl butyl ester 4 47 g 95 was isolated as a white solid mp 141 143 u00b0 c elemental analysis for c 17 h 15 cl 2 no 3 3 4 dichloro phenyl carbamic acid 4 oxo 4 phenyl butyl ester 4 32 g 12 3 mmol prepared in the previous step was suspended in 200 ml of methanol and the mixture warmed to dissolve the solid while still warm 3 3 ml of 1 n hcl 3 3 ml of water and thiosemicarbazide 1 69 g 18 5 mmol were added and the reaction stirred under nitrogen for 19 hours the solid was collected by filtration and recrystallized from isopropyl alcohol to give the title compound 2 69 g 51 as a white solid mp 195 198 u00b0 c elemental analysis for c 18 h 18 c 12 n 4 o 2 s in vivo assay male sprague dawley rats weighing 200 225 g are housed two per cage and fed purina rodent chow special mix 5001 s supplemented with 0 25 cholic acid and 1 0 cholesterol and water ad libitum for 8 days each test substance is administered to a group of six rats fed the same diet with.