id no 2 nucleotides 3727 3751 seq id no 2 nucleotides 4840 4870 reverse compliment of seq id no 2 nucleotides 5054 5080 seq id no 2 nucleotides 3249 3274 reverse compliment of seq id no 2 nucleotides 3727 3751 seq id no 2 nucleotides 4840 4870 reverse compliment of seq id no 2 nucleotides 5054 5080 m10 att mlu1 seq id no 2 nucleotides 1 25 att ecor1 reverse compliment of seq id no 2 nucleotides3727 3751 seq id no 2 nucleotides 3940 3967 att ecor1 reverse compliment of seq id no 2 nucleotides4910 4935 m12 1a att mlu1 seq id no 2 nucleotides 3940 3967 reverse compliment of seq id no 2 nucleotides 4823 4842 seq id no 2 nucleotides 4863 4887 reverse compliment of seq id no 2 nucleotides 4910 4935 m13 amp m14 oligo anealing no pcr seq id no 2 nucleotides 4843 4862 reverse compliment of seq id no 2 nucleotides 4843 4862 the pcr fragments are restriction enzyme digested by mlui and hindiii and then subcloned into mlui and hindiii sites of pgl 2b or pgl 2p promega transient transfections are carried out using cultured hela cells by calcium phosphate precitation method after 40 hours cells are harvested lysed and assayed for luciferase activity exemplary mutants are shown to a range of.