3 73 k11r g31p in blocking the residual neutrophil chemotactic activities in the samples fig4 b the untreated lesional balf samples contained 3 215 u2212 275 pg ml cxcl8 while the immunoaffmity absorbed balf contained 24 u2212 17 pg ml cxcl8 in this series of experiments the neutrophil response to the cxcl8 depleted balf samples was 65 4 u2212 4 of their responses to the unabsorbed samples it is known that cxcl8 can contribute as little as 15 of the neutrophil chemotactic activities in pneumonic mannheimiosis balf obtained from an array of clinical cases caswell et al 2001 whereas the cxcl8 depletion treatments were 99 effective in removing cxcl8 there remained in these samples substantial amounts of neutrophil chemotactic activities and the addition of 1 ng ml cxcl8 3 73 k11r g31p fully abrogated their cumulative effects fig4 b this data unequivocally confirmed that cxcl8 3 73 k11r g31p also antagonizes the spectrum of non il 8 chemoattractants expressed in these samples cxcl8 3 73 k11r g31p is highly efficacious in blocking endotoxin induced neutrophilic inflammation in vivo in our last experiments we assessed the ability of cxcl8 3 73 k11r g31p to block endotoxin induced inflammatory responses in the skin of cattle as well as the time frames over which it was effective the animals were challenged intradermally with 1 u03bcg bacterial endotoxin 15 h before internal positive control response or at three different times after.