and goettingen germany and sam2 u00ae brand membranes described in the following paragraph likewise supports including diethylaminoethyl cellulose and polyvinylidene difluoride can also be used where the binding moiety is biotin the preferred binding matrix is a support having avidin or streptavidin immobilized thereon the support may be in the form of a filter membrane beads etc the most preferred matrices are sam2 u00ae brand biotin capture membranes or sam2 u00ae brand 96 biotin capture plates 96 well microtiter format from promega corporation madison wis the sam2 u00ae brand membrane binds biotinylated molecules based on their strong affinity for streptavidin the process by which the membrane is produced results in a high density of streptavidin on the membrane filter matrix promoting rapid quantitative capture of biotinylated substrates in addition the sam2 u00ae brand membrane has been optimized for low nonspecific binding using the 96 well plate format allows washes to be performed using a vacuum manifold or a commercially available plate washer where the binding moiety is an antibody or antigenic determinant the preferred binding matrix is an affinity matrix having immobilized thereon a compound which binds strongly and specifically with the binding moiety the subject assay can be used to detect and measure the presence of any lipid or phospholipid kinase or phosphatase in short any enzyme whose catalytic activity transfers a phosphate group to a lipid or phospholipid substrate or any enzyme whose catalytic activity removes a phosphate group from a lipid or phospholipid substrate can be assayed using the present invention more specifically the subject assay can be used to determine the presence and or activity of any lipid or phospholipid kinase falling within the enzyme classification ec 2 7 1 x where x is a variable including without limitation ec 2 7 1 1.