of the medium is adjusted to 7 0 then the sterilization was carried out at 121 u00b0 c for 45 min the fermentation substrate feeding and bioconversion were carried out according to the example 2 after finishing the bioconversion the pravastatin formed in the concentration of 620 u03bcg ml was isolated as follows the ph of 5 15 liters broth containing 620 u03bcg ml pravastatin was adjusted with 2m sodium hydroxide to 9 5 value then stirred at room temperature for 1 hour the broth was filtered and the mycelium was washed with suspension in 1 u00d7 2 liters and then 1 u00d7 0 5 liters water filtrates are combined and the ph of the aqueous solution was adjusted with 20 sulfuric acid to 3 7 value and extracted with 2 5 liters then with 1 5 liters ethyl acetate the ethyl acetate extracts were combined washed with 2 u00d7 0 5 liters water and 1 95 g dicyclohexylamine was added the ethyl acetate extract was concentrated at 40 u00b0 c to 200 ml under reduced pressure and 0 195 g dicyclohexylamine was added again into the concentrate which was then stirred at 15 u00b0 c for 6 hours the precipitated crystalline material was filtered washed with 20 ml and with 15 ml ethyl acetate and dried at 40 u00b0 c in this way 3 51 g crude product was obtained after the recrystallization of the crude product in an acetone ethanol mixture 3 05 g of pravastatin dicyclohexylamine salt was obtained melting point 162 168 u00b0 c which was converted to pravastatin according to the example 5 the fermentation substrate feeding and bioconversion were carried out with the mortierella maculata n spec e 97 ncaim.