and p 0 001 respectively and pmtor not contributing significantly fig1 2 creating an optimal model resulted in a highly significant model p 0 0004 with only pten mtor and pakt in the model both contributing p 0 002 p 0 007 and p 0 001 respectively significantly from all of these models a risk continuum can be generated whereby a individual patients based on their expression levels of these biomarkers can be placed on this continuum and clinical decisions made thereof see fig1 and 14 containing 110 primary glioblastomas at two fold redundancy were formalin fixed paraffin embedded tumor samples obtained at yale university new haven hospital from 1961 1983 and was constructed at the yale university tissue microarray facility the median follow up time is 13 2 months a modified indirect immunofluorescence protocol with heat induced epitope retrieval in tris edta buffer ph 9 0 as described previously camp et al automated subcellular localization and quantification of protein expression in tissue microarrays 2002 nature medicine 11 1323 all antibodies were from cell signaling technology danvers mass staining conditions for pten antibody clone 138g6 rabbit monoclonal at 1 25 mtor antibody clone 7c10 rabbit monoclonal pmtor antibody clone 49f9.