of 100 nm the compounds were incubated for 16 hours the activation of the fusion protein gal4 dbd mr lbd leads to expression of the reporter u03b2 lactamase which is detected by the conversion of a fret substrate in a ratiometric assay format this functional assay allows for measurement of receptor agonism by compounds and can be used to determine compound potency and selectivity assay reproducibility was determined by calculating z u2032 values for untreated versus maximum stimulation the z u2032 value was greater than 0 6 indicating good reproducibility of the assay format several compounds showed dose dependent stimulation of the mr signaling pathway table 3 a 58 year old male visits his ophthalmologist for a routine check up the physician discovers that the patient exhibits an elevated intraocular pressure and is at high risk for future complications the patient is instructed to apply a topical liquid formulation containing one of the compounds in table 1 once daily to each eye the patient returns for a follow up visit three months later upon measuring intraocular pressure it is noted that the patient now exhibits a reduced intraocular pressure a 38 year old male visits his ophthalmologist complaining of irritation in his right eye the physician discovers that the patient 39 s right eye is inflamed and red the patient is instructed to apply a topical liquid formulation containing one of the compounds in table 1.