dissolved in 15 ml of distilled water at 40 u00b0 50 u00b0 c and filtered through filter paper the solution was chilled while stirring and the resulting crystals obtained were filtered and washed with a few milliliters of 96 ethanol then dried in vacuum at 40 u00b0 c the dry white crystalline powder obtained was analyzed and had a combined menadione content of 50 8 the pyridoxamine content was 24 8 expressed as free base the elemental analysis gave the following results in percent by weight ______________________________________actual theoretical______________________________________c 53 13 c 53 24 h 4 78 h 4 77 n 4 12 n 4 14 o 28 31 o 28 37 s 9 44 s 9 44 ______________________________________ the recrystallized product corresponded to the empirical formula c 30 h 32 n 2 o 12 s 2 it had a melting point of 173 u00b0 175 u00b0 c was sparingly soluble in water at room temperature 1 3 g in 100 ml of water and was poorly soluble in 99 ethyl alcohol and in chloroform less than 0 1 g in 100 ml ir and uv spectra are shown in fig1 and 2 respectively the first new compound was 2 methyl 3 hydroxy 4 aminomethyl 5 hydroxymethylpyridine bis 1 2 3 4 tetrahydro 2 methyl 1 4 dioxo 2 naphthalenesulfonate it had the empirical formula c 30 h 32 n 2 o 12 s 2 and had the molecular weight of 676 69 its structural formula was str6 the first.