examples are provided for purposes of illustration only and are not intended to be limiting unless otherwise specified various dilutions of the peptide were incubated with fixed amounts of sheep red blood cells in serum or in fibrin free serum in a suitable assay plate the plate was incubated for a period between 2 and 12 hours and the lytic activity was observed and recorded lysed red blood cells appeared as clear fluid in wells whereas unlysed cells maintained red color as rbcs remained intact for e coli time kill assays cultures were inoculated 1 10 for 90 minutes then the starter culture was diluted 1 50 and incubated for 90 minutes the peptide and control were added at this juncture t 0 bacillus anthracis sterne and bacillus subtilis were grown in bs media bsm 1 l lb 5 g nutrient broth 1 ml 1m naoh in bacillus time kill assays approximately 10 5 cfu of the test organism at mid log phase was used to inoculate 2 mls bsm and pad 1 17 and controls added total viable bacterial counts colony forming units cfu were determined by serial 10 fold dilution of culture aliquots in sterile medium followed by spread plating of 100 ml samples of each dilution.