the ct values listed in the table are the average values from the two samples the viruses were concentrated as follows i transfer 1 8 ml of the virus containing cell culture sample into a 2 0 ml test tube add 25 u03bcl of an aqueous alginate solution 1 and 200 u03bcl 1 hydrochloric acid to the cell culture medium 2 ml vortex and incubate at rt for 10 minutes ii centrifuge at 14 000 rpm for 2 minutes remove supernatant completely iii resuspend the gel fragment with 500 u03bcl of a chaotropic salt solution 4 m guanidine isothiocyanate the viral nucleic acids were subsequently isolated on an automatic extraction system innupure u00ae c12 analytik jena ag for this purpose a commercial kit innuprep virus rna kit analytik jena ag was used using the commercial kit 200 u03bcl samples were also worked up directly on an automatic extraction system innupure u00ae c12 innuprep rna kit c12 analytik jena ag thereafter the isolated nucleic acid was used for detection of the virus by means of real time pcr results of the real time pcr u2014 comparison of the u201c standard method u201d with the inventive concentration method thus it is clear that a larger sample volume can be processed with the inventive concentration method this results in the advantage of higher sensitivity especially when the number of viruses to be detected is small in this way it is shown that the inventive method offers an option for processing larger sample volumes and therefore achieving higher detection sensitivity each document patent patent application or patent publication cited by or referred to in this disclosure is.