membrane fraction were determined by radioimmunoassay the calmodulin content in the soluble fraction was significantly decreased by adding the aqueous solution of calcium chloride to the hippocampal homogenate compared with the one treated with egta on the contrary the content of calmodulin in the membrane fraction was increased by adding the aqueous solution of calcium chloride the compound a 10 u03bcm significantly suppressed the change in calmodulin content caused by calcium ion table 1 shows the results on the other hand w 7 and the compound a exerted no effect on the behavior of calmodulin in the presence of egta table 1______________________________________effect on intracellular localizationof calmodulin in hippocampus 1 calmodulin content u03bcg mg protein test group soluble fraction membrane fraction______________________________________control 4 5 u00b1 0 13 8 8 u00b1 0 251 mm egta 12 3 u00b1 0 77 7 6 u00b1 0 401 mm cacl sub 2 0 83 u00b1 0 04 11 4 u00b1 0 34 1 mm cacl sub 2 1 75 u00b1 0 52 7 1 u00b1 0 50 10 u03bcm compound a______________________________________ p lt 0 01 vs control p lt 0 05 vs 1 mm cacl sub 2 both common carotid arteries of a gerbil were ligated for 10 minutes one hour and 24 hours after.