in fig4 a and compare sequential injection in fig4 b this is due to that a dendritic complex network is formed the more the complex grow the more epitopes are available for binding of more enhancement molecules if the enhancement reagents instead are injected after each other available binding sites are saturated see sensorgram in fig4 b dendritic amplification has the potential to increase sensitivity in spr systems such as biacore systems t100 with immunogenicity package and t200 with merged injections trace amounts of analyte or enhancement molecules left on the surface after the regeneration can also be amplified so it is important with a good regeneration the wash of the flow system after injections is also important a variant of the purposed method is to inject the analyte together with one of the enhancement solutions not a separate analyte injection the analyte could be mixed with the enhancement reagent that do not bind the analyte in the example above u03b22 u03bc could have been mixed outside the instrument with anti rabbit igg u03b22 u03bc anti rabbit igg could then be injected from one channel and anti u03b22 u03bc from the other channel another variant of the purposed method is possible if the analyte has multiple binding sites for the enhancement molecule and the enhancement molecule has multiple binding sites for the analyte the analyte and enhancement should then be injected simultaneous but separately in this case a second enhancement reagent is not needed power free.