rp hplc dna sequencing reactions were generated by either solid phase bst sequencing or taq cycle sequencing the results of the substitution experiment are shown in fig3 here three dye labeled termination products i e fam tamra and rox were generated combined with either joe or bodipy u00ae 530 550 termination products and analyzed by automated dna sequencing with the exception of bodipy u00ae 589 616 reactions bodipy u00ae 503 512 bodipy u00ae 530 550 and bodipy u00ae 530 550 and bodipy u00ae 564 570 labeled termination products migrated approximately 3 4 to 1 base pair faster through the gel than fam joe or tamra labeled termination products respectively the discrepancy between the two reactions is the result of the altered mobility of the different dye structures although software modifications could have been employed to correct dye primer mobility shifts chemical modification of the r865 primer was performed fig2 oligonucleotides r930 r931 and r932 were dye labeled with the bodipy u00ae dyes listed in fig1 and purified by rp hplc as shown in fig3 increasing the linker arm length from ch 2 6 to ch 2 12 r932 or addition of one 5 39 base plus ch 2 3 r930.