present invention is to culture the white rot fungus to provide a readily available active ingredient capable of biodegrading tnt such development should take place under sterile or semisterile conditions in a stationary liquid medium containing nitrogen and sufficient nutrients for germination and rapid growth glucose cellulose and inexpensive commercially available corn cobs or a source of cellulose work equally well as a source of nutritional requirements for fungal growth the white rot fungus was incubated in a liquid culture media composed of 56 millimolers mm glucose 1 2 mm ammonium tartrate nitrogen limited trace metals and thiamine 1 milligram per liter in a 20 mm 2 2 39 dimethylsuccinate buffer ph 4 2 the liquid culture media was first sterilized by filtration through a cellulose acetate membrane filter pore size 0 22 u03bcm culture bottles for the liquid culture media were sterilized by autoclaving at 121 u00b0 c and 15 psi for 20 minutes nine milliliter aliquots of the liquid culture medium were next dispensed into each of a plurality of 250 milliliter wheaton bottles equipped with a gas exchange manifold having a teflon seal to grow the fungus a one milliliter ml 0 5 absorbance unit at 650 nanometers spore suspension of phanerochaete chrysosporium was inoculated into the liquid culture medium and grown at 39 u00b0 c.