integral tamper evident seal the cap can be reapplied but provides a clear indication that a culture unit has been partially opened or inoculated the lid assembly is a composite unit housing several features which could be a u2018 sandwich u2019 construction where the u2018 filling u2019 is a moulded rubber membrane showing different features in different areas the outer casework can be coded in various ways to identify contents and application this can include colour coding with or without labelling possibly bar coding as a computer compatible identification code the smaller components foil seals perforators and filter elements are assembled into the lid casing prior to closure and bonding into the vessel followed by gamma irradiation to sterilise the complete assembly an aspect of the culture vessel design is the selection of appropriate materials avoidance of u201c sharps u201d and widely diverse materials is intended to simplify final disposal after use for safety reasons terminal autoclaving is advisable this would be followed by combustion using methods normally employed for disposables conventional culture containers create difficulties in disposal the use of appropriate materials is intended to improve the environmental impact of large scale usage and disposal in one aspect the container with associated components contents and pressure transfer diaphragm is made from materials selected for effective and environmentally sound disposal the durable part of the culture system takes the form of a compact dedicated instrument in.