systems deutschland gmbh amp co kg or emultop degussa texturant systems deutschland gmbh amp co kg the heat exchanger of a high pressure homogenizer max output 5 l hr highest pressure 1200 bar model hs 1002 manufactured by hwasung machinery co ltd south korea was placed in ice water such that the temperature of the outlet of the homogenizer did not exceed 30 u00b0 c in the meantime the inside of the homogenizer was then washed with distilled water so as to be ready to operate then hpf was dissolved in a buffer solution 20 mm nah 2 po 4 ph 6 5 7 5 1 mm edta at a concentration of 1 mg ml phospholipid was added at a ratio of 10 w v and sufficiently hydrated and stirred the stirred solution was passed through the homogenizer three times or more at room temperature and a low pressure of 0 bar to the solution passed through the homogenizer phospholipid was added to a ratio of 14 w v and sufficiently hydrated and stirred the stirred solution was passed through the homogenizer three times or more at 100 bar then to this solution phospholipid was added to a ratio of 18 w v sufficiently hydrated and stirred and passed through the homogenizer three times or more at 200 bar then to this solution phospholipid was added to a ratio of 20 w v sufficiently hydrated and stirred and passed through the homogenizer three times or more at 300 bar then to this solution phospholipid was added to a ratio of 22.