for 16 17 h with mixing on a rotisserie at 60 rpm following hybridization the solutions were removed and the arrays were rinsed with 1 u00d7 mes the arrays were washed and stained using the genechip fluidics station protocol eukge_ws2 which consists of 10 cycles of 2 mixes per cycle with non stringent wash buffer 6 u00d7 sspe 0 01 tween 20 at 25 u00b0 c followed by 4 cycles of 15 mixes per cycle with stringent wash buffer 100 mm mes 0 1 m na and 0 01 tween 20 at 50 u00b0 c the probe arrays were stained for 10 min in streptavidin phycoerythrin solution sape 1 u00d7 mes solution 10 u03bcg ml sape molecular probes eugene oreg and 2 u03bcg u03bcl acetylated bsa invitrogen at 25 u00b0 c then washed for 10 cycles of 4 mixes per cycle at 25 u00b0 c the probe arrays were treated for 10 min with an antibody solution 1 u00d7 mes solution 2 u03bcg u03bcl acetylated bsa 0 1 u03bcg u03bcl normal goat igg sigma chemical st louis mo 3 u03bcg u03bcl biotinylated goat anti streptavidin antibody vector laboratories burlingame calif at 25 u00b0 c followed by a second staining for 10 min in sape at 25 u00b0 c the final wash was 15 cycles of 4 mixes per cycle at 30 u00b0 c with non stringent wash buffer the probe arrays were then scanned once at 1 56 u03bcm resolution using the affymetrix genechip scanner 3000 or at 3 u03bcm resolution using.