in dry ice and are irradiated while frozen cell viability after the dispersion process is assessed with trypan blue dye solution five to ten million of live tumor cells are needed for one vaccination 4 estimation of the immune reaction to autologic cancer cells developing concomitantly with the vaccination to estimate the immune reaction to autologic cancer cells developing concomitantly with the vaccination the delayed type hypersensitivity reaction was used for this purpose gamma irradiated tumor cells 200 gy without virus were administered to patients following 24 hours post injection the diameter of erythema appearing after administration of the cells was measured the reaction was considered to be positive where the erythema diameter exceeded 7 mm while the number of the vaccinations increases for each patient and in accordance with the regular stimulation of the immune response to cancer cells the erythema should increase in size medical assessment report on surgical treatment combined with immunotherapy of iii iv stage cancer patients issued to chief researcher at the cancer research center of the russian academy of medical sciences v m senin md phd in medical science this assessment report presents results of monitoring cancer patients with solid tumors who were admitted to the thoraco abdominal department of the st petersburg city hospital no 26 in incurable and inoperable.