system does not allow proper post translational processing folding etc which would normally occur in cellular translation thus it may be that proper disulfide linkages do not always form in the in vitro system leading to formation of dimer in some cases labeled proteins generated in the above described in vitro expression system were next tested for lps binding activity wells of microtiter plates were coated with lps from salmonella minnesota r7 rd mutant 5 mg ml in methanol stock culture in 0 1 m na 2 co 3 20 mm edta ethylenediamine tetraacetic acid at ph 9 4 a total of 2 u03bcg lps in a 50 u03bcl well following overnight incubation at 4 u00b0 c the wells were rinsed with water and dried at 37 u00b0 c the wells were then blocked with 215 u03bcl dulbecco 39 s pbs 0 1 bsa for 3 hours at 37 u00b0 c the blocking solution was then discarded and the wells were washed with pbs 0 2 tween 20 the rbpi samples were then added 2 u03bcl of the translation reactant to a 50 u03bcl total volume in pbs 0 2 tween following overnight incubation at 4 u00b0 c the wells were washed 3 times with pbs 0 2 tween and the amount of labeled protein remaining in each well was determined by liquid scintillation counting the results demonstrated that approximately equivalent lps binding took place for all three bpi species referred to above rbpi 1 199 displayed binding of 48 690 cpm rbpi 1 199 ala 132 displayed binding of 59 911 cpm and rbpi 1 199 cys 135 displayed binding of 52 537 cpm each of the aforementioned values represents the average of triplicate determinations the average binding of the control no dna was 5 395 cpm.