gene we anticipate that the approach described above or other methods for measuring allelic variation in gene expression will play a major role in defining normal human variation and disease susceptibility in the future 3 lymphoblastoid cell lines representing two genetically unrelated individuals from each of 48 ceph references families were obtained from the national institute of general medical sciences repository maintained by the coriell institute for medical research cells were grown in rpmi with 10 fbs and mrna was isolated from 2 u00d7 10 6 cells using amersham pharmacia quickprep micro mrna purification kit rt pcr products from each allele of the gene of interest were distinguished using abi prism snapshot multiplex kit and analyzed on a spectrumedix sce9610 genetic analysis system sequences of the primers used for pcr amplification and snp determination are available upon request 5 the fractional allelic experiment for each sample was determined through seven replicates prior to subsequent statistical analyses obvious technical failures or statistical outliers were eliminated in no case did this result in elimination of more than three replicates and on average resulted in elimination of one in every 25 data points the data were then analyzed using the mixed procedure of the sas system version 8 0 for repeated measurements this analysis revealed that none of the 17 individuals tested for expression of apc had a fractional allelic expression value that exceeded the 95 confidence interval for the mean in contrast the.