process scalability and prove that the least water soluble compounds were the active compounds fig6 shows the chromatogram of the fast centrifugal partition chromatography of 10 g of agave syrup diluted in 10 ml of the same mobile phase of the previous experiment once more it was possible to separate the compounds contained in the agave syrup in two principal fractions furthermore it was proved that the compound separation by fast centrifugal partition chromatography was scalable even though there was a rising in the quantity of agave syrup the obtained partition coefficients were not affected in this experiment in order to carry out a bioassay of the fractions there were selected colon cancer cells in order to observe the inhibitory action of said fractions fig7 shows that the samples with the greatest partition coefficient were the samples that showed the greatest colon cancer cell inhibitory action in order to carry out a tentative identification of the active components of the fraction obtained by cpc it was analyzed by hplc ms using a column of c 18 zorbax 2 1 u00d7 30 mm 3 5 u03bcm the mobile phase comprised methanol and water which was modified with 5 mm of ammonium acetate at a flow of 0 5 ml min the column was balanced with 40 of methanol and a gradient was programmed in such way that at a time of 12 5 minutes the methanol percentage was incremented to 90 and maintained at said percentage during the last 5 minutes of the.