72 while specific sample beads have been described it is within the present invention that the chambers of the container 70 may be filled with sample beads of all forms and sizes which can be specifically manufactured for high affinity chromatography the beads can also have high affinity chromatography the chemical and mechanical stability of the support resin and its linkage to the ligand antigen play a key role in affinity chromatography preferably as has been previously described the module bead chambers hold high affinity resin beads with specific antibodies or antigens immobilized onto the solid phase resin e g actigel ald protein a protein g etc so that antigens in the sample can bind to their specific antibodies or alternately the antibody complexes can become bound while passing through the resin module and become immobilized as well the principle of affinity chromatography requires that a successful separation of a biospecific ligand is available and that it can be chemically immobilized to a chromatographic bed material the matrix numbers of methods well known in the art have been used to couple or immobilize the antigen to a variety of activated resins examples of immobilization techniques which exhibit variable linkage are those formed by the reaction of the reactive groups on the support with amino thiol hydroxyl and carboxyl groups on.