using a tri garb b2910tr liquid scintillation analyzer perkinelmer briefly brain slices or primary cortical cells were incubated with 0 8 u03bcm 14 c l cystine perkinelmer at 37 u00b0 c for 10 min the uptake was terminated by rapidly rising cells two times with ice cold unlabelled uptake buffer the cells were then lysed by adding 0 8 ml of 0 2 n naoh containing 1 sos for radioactivity determination using a tri garb b2910tr liquid scintillation analyzer perkinelmer apogsh u2122 glutathione detection kit biovision was used to evaluate cellular glutathione level according to the manufacturer 39 s instruction brain slices or primary cortical cells were incubated with 200 u03bcl of buffer solution containing 5 33 mm kcl 26 19 mm nahco 117 24 mm nacl 1 01 mm nah 2 po 4 2 0 mm cacl 2 5 56 mm d glucose 100 u03bcm cystine with or without 25 u03bcm imatinib 10 u03bcm sorafenib 10 u03bcm regorafenib 10 u03bcm erastin or 500 u03bcm sulfasalazine sas incubated in 95 o 2 and 5 co 2 for 1 h at 37 u00b0 c samples were diluted in 20 mm borate buffer at ph 9 0 and were derivatized for 1 min with n tert butyloxycarbonyl l cysteine and o phthaldialdehyde samples then were separated in a 5 mm c18 reverse phase column 220 u00d7 4 6 mm sheri 5 brownlee and glutamate was monitored by fluorescence 334 nm excitation and 433 nm emission using an rf 10axl fluorescence detector shimadzu standards of glutamate were assayed before and after the dialysis samples.