dilution the concentration of the components in the sealer protein solution was as follows the sealer protein solution was diluted in hank 39 s buffered saline solution hbss without ca 2 or mg 2 the presence of these two ions induced the formation of precipitates during the freezing and thawing process those skilled in the art will recognize that the tisseel sealant can be supplemented with other commercially available fibrinogen and aprotinin preparations to achieve similar results moreover fibrinogen may also be diluted to other concentrations depending on the mode of administration to enhance the polymerization characteristics following dilution the thrombin solution obtained from baxter contributes the following to the cryoprotected solution hbss with ca 2 and mg 2 was the chosen diluent the desired cell mixture ratio and concentration was resuspended in the cryoprotected solution to obtain the component 2 various keratinocyte fibroblast ratios have been considered including 1 1 1 3 1 4 1 9 to as high as 1 50 moreover various final cell concentrations in component 2 considered have been 1 million 2 5 million 5 million 10 million 20 million and 50 million cells ml final concentration previous work has shown the efficient concentrations of mitomycin c mmc for growth arrest of mouse 3t3 fibroblasts to be 2 u03bcg ml rheinwald and green cell 6 331 43 1975 and for growth arrest of human dermal fibroblasts to be 8 u03bcg ml limat et.