preparation for positive and negative samples to be used in the evaluation of the kit positive samples are obtained by volume of 5 ml by venipuncture into vacuum tubes with edta anticoagulant from patients with positive clinical diagnosis of malaria by p vivax or p falciparum whose density is not less than two crosses using the semi qualitative scoring method in crosses for diagnosis of malaria manual of laboratory procedures for the diagnosis of malaria standards technical series n u00b0 39 lima 2003 for either case p vivax and p falciparum the sample is obtained from the malaria infected patients before initiating treatment negative samples are also obtained by venipuncture into vacuum tubes with edta from clinically healthy persons negativity is confirmed through a test of thick film through microscopic observation to endorse him as a person without malaria positive blood samples are obtained in endemic areas of malaria by p vivax and p falciparum and transported by the analyst the place where the test is performed by maintaining the cold serie at 2 u00b0 c to 8 u00b0 c carriage conditions of samples are realized under bio security standards for the transportation of samples transportation is executed in bio security containers validated for 72 h at temperatures from 2 u00b0 c 8 u00b0 c samples should reach the laboratory where the assay sample is executed in a period no longer than 48 h from obtained.