t and b cell proliferative responses though not as many divisions were seen as with specific antigen stimulation likely because the cells were only cultured with the mitogen for 3 days the specificity of the c albicans stimulated b cells was demonstrated by elipsot fig2 this experiment suggests that a 1 u00d7 stimulation with c albicans did give rise to a small population of antibody producing cells u02dc 0 2 of total b cells that was not detected in untreated cultures or those stimulated with tt left and middle wells this discrepancy between the frequency of proliferating cells and c albicans specific b cells detected by elispot could be the result of several factors a likely explanation is that we used a crude c albicans whole antigen extract containing u02dc 19 carbohydrates by weight while c albicans polysaccharides are strong inducers of b cell responses only protein antigen specific responses would be detected in the elispot assay tetanus specific antibodies were detected in another elispot experiment where the cell donor 39 s serum anti tetanus level was higher 63 u03bcg ml and dcs were cultivated in xvivo 15 medium all other components concentrations and ratios were left unchanged except that of the number of cells deposited per elispot.