the container was completely filled with ringer 39 s solution using a sterile syringe 10 ml ringer 39 s solution were necessary in order to fill the free space between the explanted hip prostheses 5 and the inside wall of the container body 1 the container was vortexed for 30 seconds using a vortex mixer and then subjected to sonication frequency 40 u00b1 2 khz and power density 0 22 u00b1 0 04 kv cm 2 in an aqua sonic model 750 t ultrasound bath vwr scientific products for 5 min followed by additional vortexing for 30 seconds the resulting sonicated fluid was removed under sterile conditions with a sterile syringe from the container without opening the container the sonicated fluid was plated in 0 5 ml aliquots onto aerobic and anaerobic sheep blood agar plates for comparison synovial fluid was obtained from a subject and inoculated in 0 1 ml aliquots onto aerobic and anaerobic shape blood agar plates bd diagnostic systems the aerobic and anaerobic sheep blood agar plates from both sample types were incubated at 35 to 37 u00b0 celsius in 5 to 7 carbon dioxide aerobically and anaerobically for 5 days and 7 days respectively always 6 parallel samples were analysed from both sample groups synovial fluid samples and sonication samples respectively the above results show that with the container of the present invention a concentration of pathogens eluted from an explanted hip prostheses occurs some of the pathogens are either not detectable in synovial fluid or are detectable but without reliability in contrast due to the concentration effect achieved by using the container of the present invention in combination with ultrasound techniques it is possible to detect and diagnose pathogenic infections in almost each sample with high reliability to better illustrate the container and method.