beforehand as well as the isolator 12 the pass box 14 is also provided with a window 26 on its front face and a pair of gloves 28 for example is provided on the window 26 for manipulating objects inside the pass box 14 further on the side wall of the pass box 14 that faces the door 22 r a door 30 for transferring objects is provided when transferring objects inside the aseptic manipulation chamber 13 which is maintained in an aseptic condition the objects are temporarily housed inside the pass box 14 and decontaminated or sterilized before communication is allowed between the aseptic manipulation chamber 13 and the pass box 14 in order to transfer the object s inside the aseptic manipulation chamber 13 further the incubator 16 is detachable from the isolator 12 but the incubator 16 is in communication with the aseptic manipulation chamber 13 when it is attached to the isolator 12 so that cells can be placed inside the aseptic manipulation chamber 13 to cultivate for a predetermined period with reference to fig2 and 3 the structure of the doors 22 r and 22 l of the present embodiment will be explained fig2 is a front view of the closed door 22 r viewed from a position inside the aseptic manipulation chamber 13 of the isolator 12 fig3 a is a cross sectional view along line b b of fig2 and fig3 b is a cross sectional view along the line a a of fig2 incidentally the structure of the door 22 l is the same as that of the door 22 r except for reversing the right and left therefore the explanation for the door 22 l is omitted the aseptic manipulation chamber 13 of the.