to have the ability to inhibit the microtubule re assembly and interfere mitosis on cellular level the method was primarily described here below a549 cells were seeded in a confocal culture dish at 8 u00d7 10 4 cells dish grown for 24 h and divided into two groups dmso 0 5 was added to one group followed by culture for 24 h compound 3 31 nm was added to the other group followed by culture for 24 h then cells of two dishes were fixed directly 37 u00b0 c the remaining cells were placed in ice for 1 h then at 37 u00b0 c for 0 5 10 and 15 min cells were then fixed with paraformaldehyde 4 for 15 min then cells were subject to permeabilization buffer for 15 min primary u03b2 tubulin mouse antibody was added and incubated at room temperature for 1 h cells were incubated with dylight 549 conjugated goat anti mouse igg secondary antibody along with dapi stain nucleus for 30 min photographs were obtained by laser scanning confocal microscope fig1 showed the effect of compound no 3 on microtubules microtubule re assembly and mitosis a c f was the solvent control group 0 5 dmso added b g j was test group 31 nm compound no 3 added scale bars 10 u03bcm as shown in fig1 the filiform structure of microtubule was affected after the addition of compound 3 and microtubule depolymerized to form discreet tublin however microtubule was automatically deploymerized into an meshy state in the ice incubation system with the temperature increasing tublin was automatically re assemble into microtubule in the system without compound.