carbon after 15 minutes the mice were sacrificed and their intestines from the stomach to the cecum were dissected the total length of the intestine as well as the distance traveled from the stomach to the charcoal front was measured for each animal and the results are expressed as the percent of the total length of the intestine traveled by the charcoal front all results are reported as the average of 10 mice u00b1 standard deviation a comparison of the distance traveled by the charcoal between the mice treated with peptide versus the mice treated with vehicle alone was performed using a student 39 s t test and a statistically significant difference was considered for p lt 0 05 p values are calculated using a two sided t test assuming unequal variances as can be seen in fig3 a and fig3 b wild type st peptide seq id no 4 sigma aldrich st louis mo 0 1 mg kg synthetically manufactured seq id no 3 and zelnorm u00ae 0 1 mg kg a drug approved for ibs that is an agonist for the serotonin receptor 5ht4 increase gastrointestinal transit rate in this model fig4 a shows the result of a study demonstrating that intestinal transit rate increases with an increasing dosage of either recombinantly synthesized seq id no 4 or seq id no 5 fig4 b shows the results of a study demonstrating both chemically synthesized seq id no 4 or seq id no 3 peptide increase intestinal transit rates more.