throughout the entire 15 seconds of 1v application the normalized peak conductance for the triplex hybridization of the antiparallel pna probe with a 1 bp mismatched dsdna seq id no 2 probe no 1 and with the 2 bp mismatched dsdna seq id no 3 probe no 1 were respectively 79 and 96 lower than that observed with the perfectly matched dsdna pna triplex hybrid seq id no 1 probe no 1 during the first second of voltage application fig1 a similar percent decreases in conductance between perfectly complementary triplexes and triplexes containing base pair mismatches were obtained when the conductance values over the entire 15 seconds of voltage application were averaged in fig1 a the 1 bp and 2 bp mismatched dsdna pna hybrids resulted in average conductance values that were 65 and 91 lower respectively than those for the perfectly matched dsdna pna hybrid all experiments expressed in fig1 a were carried out at room temperature 23 u00b0 c as the degree of mismatch between the probe and the double stranded target increased the level of intercalation by yoyo 1 diminished and the level of conductance decreased these relationships were also observed when the experiments referred to above were repeated and a higher voltage 5v was applied during the 5v application the normalized average conductance values for the 1 bp mismatched dsdna pna triplex seq id no 2 probe no 1 and the 2 bp mismatched dsdna pna triplex seq id no 3 probe no 1 were respectively 52 and 67 lower than that observed for the perfectly matched dsdna pna triplex seq id no 3 probe no 1 fig1 b experiments expressed in fig1 b were performed at room temperature 23 u00b0 c when.