isolating the recombinant pnf034 plasmid and digestion with bamhi confirmed transformants were designated as tnf006 in yet another embodiment of the invention the ability of yeast transformants tnf006 to produce ethanol was verified under fermentative conditions and compared with that of the parent w303 cells the parent yeast strain was cultured in complete synthetic medium containing 0 67 yeast nitrogen base 10 glucose and supplemented with amino acids uracil and adenine hemisulphate as specified in gietz lab protocol available in public domain http www umanitoba ca faculties medicine biochem gietz media html while culturing tnf006 leucine was excluded from the culture media to ascertain auxotrophic nature of the transformant equal number of cells 5 u00d7 10 6 cells were cultured in sealed bottles containing 500 ml of media as specified above at 28 u00b1 2 u00b0 c for 48 hours aliquots 50 ml were withdrawn at specified time periods and evaluated for growth glucose consumption and alcohol production in order to obtain genetically transformed microorganisms endowed with simultaneous enhancement of reduction potential and reductive enzyme activities the recombinant plasmid pnf034 was constructed this plasmid contains the gene cassette encoding glyceraldehyde 3 phosphate dehydrogenase followed by alcohol dehydrogenase of saccharomyces cerevisiae both driven independently by adh1 promoter the attached gene sequence depicts the alignment of amino acid sequence deduced from nucleotide sequence of the excisable gene cassette encoding glyceraldehyde 3 phosphate dehydrogenase followed by alcohol dehydrogenase of saccharomyces cerevisiae both driven independently by adh1 promoter the nucleotide sequences 1 1516 denote adh1 promoter the nucleotide sequences 1517 1559 denote kozak sequences upstream to tdh3 gene the nucleotide sequences 1560 2558 denote the open reading frame orf of tdh3 gene the nucleotide sequences 2559.