in the presence of a laccase and a xylanase the activity of the xylanase pulpzyme hc novozymes a s is 1000 units per ml enzyme solution one xylanase unit is defined as the amount of enzyme which under standard conditions ph 9 0 50 u00b0 c 30 minutes of incubation release one micromole of the dye from a dyed rbb xylan n benzylidenebenzylamine was dissolved in ethanol and then mixed with a phosphate buffer and a chicago blue solution a solution of laccase ns51003 novozymes a s and xylanase pulpzyme hc novozymes a s was added to make 1 ml of the final solution containing 20 um of the mediator 20 mm buffer at ph 7 0 and chicago blue solution with absorbance at a610 nm between 0 6 to 0 8 after the enzyme was added the change in the absorbance was measured immediately using a uv spectrophotometer uv 1201 shimadzu scientific instruments the decrease in absorbance was recorded after 3 minutes and was used to estimate the results of bleaching the results are shown below concentration laccase xylanase ma610 mediator u03bcm v v v v 3 min none 0 0 5 0 5 71 n benzylidenebenzylamine 20 0 5 0 11 n benzylidenebenzylamine 20 0 0 5 112 n benzylidenebenzylamine 20 0 5 0 5 163 it is to be understood that the above described embodiments are illustrative only and that modification throughout may occur to one skilled in the art for example a person of skill in the art will recognize that the mediators.