injected subcutaneously 2 million cells site in 50 u03bcl culture medium for lk2 cells 0 5 million for b16 into 4 sites each on the flanks of nude mice for lk2 cells or c57bl 6 mice b16 mouse melanoma cells when tumors became visible 2 4 mm diameter five to 10 days after injection of cells 37 u03bcg of ebi 46 in 25 u03bcl of isopropanol gel was applied to each tumor site each day for 3 days tumor size was measured with calipers at appropriate intervals squamous cell carcinoma scc tumors were established by the same method as for topical treatment but were allowed to grow for 15 days before drug treatment 7 u03bcg ebi 46 in 20 u03bcl saline was injected into each of 3 sides of the tumor tumor size was measured with calipers at appropriate intervals in order that the invention may be readily understood and put into practical effect particular preferred embodiments will now be described by way of the following non limiting examples biomass samples including seeds leaves and bark from fontainea picrosperma where collected and subject to the following extraction process these samples and their subsequent fractions are referred in the below example as eb548 the biomass is generously covered with methanol and shaken u02dc 2 l overnight followed by filtration to give the first extract this process is repeated a second time u02dc 2 l u02dc 5 hours to generate the second extract each extract is examined by analytical hplc and bioassayed fig1 the sequential methanol extracts are combined and the solvent removed by rotary evaporation to afford an aqueous concentrate the aqueous concentrate from the extraction is diluted with.