so the endophyte can be detected by this procedure as a result neotyphodium endophyte was detected with almost no branching in the intercellular spaces of plants of the genera poa festuca agrostis milium elymus glyceria bromus brylkinia brachypodium phleum and digitaria gliocladium endophyte was detected with multiple branching in the intercellular spaces of genus lolium the endophyte in the aforesaid section 1 was isolated by the following method from the various plants in which it was detected to isolate the endophyte from the plant the leaf and leaf sheath were washed with water immersed in a 70 aqueous solution of ethanol for 10 seconds immersed in a 2 5 aqueous solution of sodium hypochlorite for 10 minutes washed three times with sterile water transferred to an endophyte isolation culture and cultivated in the dark at 25 u00b0 c the isolation culture was prepared by sterilizing pda potato dextrose agar adjusted to ph 5 6 at 121 u00b0 c for 15 minutes adding 100 mg l each of penicillin and streptomycin and pipefting 20 ml portions into each plastic petri dishes of diameter 9 cm after approx 3 8 weeks the mycelium was isolated from an explant and the colonies were removed by a cork borer of diameter 5 mm transferred to a pda culture and grown 3 classification and identification of endophytes by using the flat plate culture method the mycelium transplanted to the pda culture was grown in the dark at 25 u00b0 c and the colonies formed were examined on examination the colonies on the culture were all white filaments their growth was relatively slow and they grew to a radius of only about 3 cm in one month of these the 21 endophytes which were isolated were deposited at.