ch 2 ch 2 u2014 1 577 3h s c19 ch 3 1 650 1 780 4h m u2014 ch 2 ch 2 ch 2 u2014 1 797 1 845 1h dd j1 14 8 hz j2 4 63 hz h 12 2 063 3h s ch 3 co 2 090 2 200 1h r h 16 2 220 2 327 1h m h 15 2 370 2 426 3h m h 16 ch 2 ch 2 cooh 2 539 2 610 1h m h 12 2 794 3h broad nch 3 2 907 3 030 2h m h 14 ch 2 och 3 3 116 3 243 2h m h 15 ch 2 och 3 3 254 3h s ch 3 o 3 387 3 560 2h m broad nhch 2 4 051 2h broad oh 4 485 1h dd j1 2 hz j2 7 6 hz h 1 6 063 1h dd j1 4 8 hz j2 7 2 hz h 11 8 177 1h s h 20 this compound is 2a see fig4 wortmannin 42 8 mg 0 1 mmol 6aminohexanonic acid referred to as 6 nh hexanoic acid 65 5 mg 0 5 mmol and triethylamine 20 u03bcu were mixed in anhydrous dmso 1 ml the mixture was stirred at room temperature till complete tlc monitoring the final solution was purified by reverse phase hplc with acetonitrile and water as solvents the injection mixture was made by mixing reaction mixture with acetonitrile.