hour culture and then diluted at a 1 100 ratio this diluted culture was then grown for an additional two hours after which the silver diphenyl hydantoin was added evaluation of the culture took place 18 hours after the addition of the silver ammonium phenytoin the results are summarized in accompanying table 1 table 1______________________________________effect of silver ammonium phenytoin on variousmicroorganisms minimal inhibitory concentrationmicrograms of silver ammonium diphenyl hydantoinper millilterorganism 1 10 20 40______________________________________enterococcus group d strepcandida albicans klebsiella seratia pseudomonas aeruginosastaphloccoccus aereus______________________________________ inhibition organisms grown in millerhinton broth a 24 hour culture was diluted 1 100 and compound was added to a two hour culture of this dilution and evaluated at 18 hours antibacterial action was also demonstrated in pure cultures of organisms on blood agar plates experiments designed to compare the effect of sodium sulfadiazine silver sutfadiazine and silver ammonium phenytoin were performed the parameters of the experiments were identical to those set forth in example i and the results are set forth in table 2 table 2__________________________________________________________________________minimal inhibitory concentrationsenterococcus candida a klebsiella serratia m pseudomonas a strep as ss sp s ss sp s ss sp s ss sp s ss sp s ss sp__________________________________________________________________________micro gram ofagent 1 10 20 u00b1 40 u00b1 __________________________________________________________________________ organisms were grown in millerhinton broth a twentyfour hour culture was diluted 1 100 compounds were added to two hour cultures and examined for inhibition at 18 hours the magnitude of inhibition is estimated and indicated by s sodium sulfadimene ss silver sulfadiazine sp silver ammonium diphenyl hydantoin.