ph 7 0 the reaction was carried out for 10 min by addition of 3 cyanopyridine under standard conditions the enzyme was stable between ph 4 5 and ph 10 0 fig7 the reaction was carried out in a reaction mixture 30 ml comprising 500 mm 3 cyanopyridine 40 mm potassium phosphate buffer ph 7 0 and resting cells dry weight 2 3 mg during the reaction 3 cyanopyridine 500 mm was added 7 times as soon as it was consumed in this manner 4 0 m 3 cyanopyridine was added in the course of 15 h and 3 89 m 475 g l nicotinamide was formed corresponding to a yield of 97 3 nicotinic acid was not formed 5 fatty acid pattern iso anteiso branched and 2 hydroxy fatty acids small amounts of 10 methyl branched fatty acids were additionally detected this fatty acid pattern was found in all representatives of the genus amycolatopsis fatty acid pattern 3f the combination of these chemical features is diagnostic of all species of the genus amycolatopsis the fatty acid data of the two cultures were compared with the aid of main component analyses using the entries in the fatty acid database using this method it was possible to assign both ne31 and na40 to the genus amycolatopsis an identification of the species however was not possible since the correlation factor was too low the comparison of the fatty acid patterns of both strains showed however that they are two strains of different types the result was confirmed by the results of the 16s rdna sequence analysis here too assignment to the genus amycolatopsis took place but not to any of the amycolatopsis species described in this method the.