the forward displacement primer f and the nucleic acid target molecules to use the dna polymerase extension displacement primer f with the displacement function and the extension strand of the displacement forward amplification primer f to generate the fixed forward strand 5 u2032 end the hybridization may be carried out between the displacement amplification primer extension strand as the template and the backward amplification primer r at whose 5 u2032 end there is the hybridization identification sequence of the forward primer f the double stranded nucleic acid may be synthesized by the dna polymerase extension backward amplification primer r with displacement function the hybridization is carried out by using the backward displacement primer r and nucleic acid target molecules to use the dna polymerase extension displacement primer r with the displacement function and the extension strand of the backward amplification primer r to generate the fixed backward strand 5 u2032 ends both ends of the generated backward amplification primer r extension strand have been fixed at this time the hybridization sequences of the amplification primers f and r are introduced into the 3 u2032 end and the 5 u2032 end respectively so as to act as the templates for rapid.