a ym3 microcon spin filter amicon for 20 min at 10 000 rpm at 4 u00b0 c in a beckman microcentrifuge r model bench top centrifuge the filtrate was discarded and the retained solution which contained the two peptides of interest was analyzed further by tandem mass spectrometry to deduce their amino acid sequences tandem mass spectrometry was performed at the university of manitoba 39 s winnipeg manitoba canada mass spectrometry laboratory using the procedures that are well known to practitioners of the art as a result of these procedures the disease specific marker identified by the sequence sskithrihwesasll was found this marker is characterized as a c3f fragment from the complement system having a molecular weight of about 1865 daltons the characteristic profile of the marker is set forth in fig2 as easily deduced from the data set forth in fig1 this marker is indicative of an individual suffering from myocardial infarction intracerebral hemorrhage congestive heart failure or type ii diabetes in accordance with various stated objectives of the invention the skilled artisan in possession of the specific disease specific marker as instantly disclosed would readily carry out known techniques in order to raise purified biochemical materials e g monoclonal and or polyclonal antibodies which are useful in the production of methods and devices useful as point of care rapid assay diagnostic or risk assessment devices.