that abc 1 possesses broad spectrum anti biofilm activity against multiple bacterial pathogens abc 1 is active against v cholerae and p aeruginosa at nm concentrations the ic 50 to inhibit biofilm formation for both v cholerae and the p aeruginosa cf 145 was determined using the mbec assay by measuring biofilm formation of these bacteria in duplicate for a range of abc 1 concentrations the data were fit to a log inhibitor vs response curve using a nonlinear regression with variable slope analysis by the software prism the ic 50 value of abc 1 to inhibit biofilm formation of v cholerae was 32 3 nm with a 95 confidence interval from 14 1 to 74 4 nm similarly the calculated ic 50 value of abc 1 to inhibit biofilm formation of p aeruginosa was 45 9 nm with a 95 confidence interval of 44 3 to 47 5 nm fig4 thus abc 1 possesses anti biofilm activities at nm concentrations and inhibits biofilm formation at a similar concentration in both v cholerae and p aeruginosa abc 1 inhibits biofilm formation of v cholerae under flow conditions the results obtained thus far showed that abc 1 possessed broad spectrum anti biofilm activity towards inhibition of biofilm formation in static conditions however natural biofilms and biofilm associated with disease.