use or elution modes for each mobile phase tubing wound in a clockwise direction from the center or top can result in the stationary phase being retained in the conditions summarized in table 1 the non polar solvent systems used primarily for small molecules have different stationary phase retentions for the elution modes because of different hydrodynamic properties thus the method of use of the subject spiral tube support apparatus is different with respect to polar solvent systems which have heretofore been difficult to use in countercurrent chromatography the top of the spiral tube support can be the inner terminal into which the lower phase is introduced and the bottom of the spiral tube support can be the outer terminal into which the upper phase is pumped when used as the mobile phase the inner terminal is the head when the rotation is clockwise or in the same direction as the winding direction of the spiral the outer terminal is the tail under these conditions the head and tail ends are reversed when the rotation is counterclockwise the inner terminal becomes the tail when the rotation is counterclockwise there can be high retention of the stationary phase under those conditions the same elution modes can be used with the two phase aqueous solvent systems u201c tpas u201d such as polyethylene glycol phosphate solutions table 2 lists the results of using.