with the various markers were counted manually under multi parametric fluorescence microscopy sections of the fabric scaffold excised from cultures after termination on days 19 and 42 were processed by staining directly hema 3 stain or staining with h amp e after paraffin embedding and sectioning or by sem the progress of the cultures was assessed by glucose and lactate analyses shown in fig2 3 fig2 illustrates the rate of glucose consumption between sampling intervals for each test group defined in table 2 values for intervals through day 19 represent the average from duplicate cultures while values from intervals beyond day 19 are from single cultures only glucose consumption rates increased dramatically and to a similar extent across the entire duration of the study for cultures from all three test groups suggesting that scaffold type and orientation had minimal impact on the rate of culture establishment and expansion fig2 fig3 illustrates the concentration of lactate in the circulating culture medium for each test group defined in table 2 values for time points through day 19 represent the average from duplicate cultures while values from time points beyond day 19 are from a single culture only the vertical drops on days 7 13 17 21 23 28 33 and 40 represent dilution of lactate due to culture feeding partial medium exchanges likewise no difference was seen between test groups with respect to lactate production fig3 microscopic examination revealed that tumor cultures were successfully established in all six of the realbio d4 u2122 culture systems seeded with dissociated tumor tissue regardless of the variation of scaffold material or orientation observations on day 19 revealed an even distribution of cells across the.