37 1085 1 h nmr cdcl 3 u03b47 30 u2013 7 55 m ph u03b45 15 s ch u03b42 10 s ch 3 conducting the above reaction at different temperatures and pressures for 4 hours gave the following conversions to the following is a comparison of the supercritical fluid system using carbon dioxide with the organic solvent system disclosed in our earlier application benzaldehyde 0 137 g 1 3 mmol sodium pyruvate 2 168 g 19 7 mmol ph 6 citrate buffer 5 4 ml and yeast 5 4 g were placed into a 250 ml stainless steel pressure vessel this vessel was pressurised to 1500 psi by pumping dried liquid carbon dioxide into the vessel the vessel was then stirred in a 35 u00b0 c water bath for 3 h after 3 h the reaction vessel was cooled to room temperature and slowly de gassed the vessel contents and residue was washed three times with diethyl ether and filtered gas chromatography analysis revealed 84 conversion to phenylacetylcarbinol chiral gas chromatography gc showed a ratio of 87 13 74 ee 1 h nmr cdcl 3 u03b4 7 30 u2013 7 55 m ph u03b4 5 15 s ch u03b4 2 10 s ch 3 benzaldehyde 0 137 g 1 3 mmol sodium pyruvate 2 1685 g 19 7 mmol ph 6 citrate buffer 5 4 ml and yeast 5 4 g were placed into a 250 ml stainless steel pressure vessel this vessel was pressurised to 1500 psi by pumping dried liquid carbon dioxide into.