from donor and recipient archival blood samples in the hla lab would be used for sequencing sirp u03b1 using standard sequencing facilities the donor recipient frozen blood sample pairs would be given a unique anonymous id lacking any diagnosis or any outcome details particular attention would be paid to sequence exon 3 of this gene which encodes the igv domain that is critical to ligand binding and function and also to sequence two additional exons that encode the intracellular regions of the gene which include important amino acid residue for conveyance of signals emanating from sirp u03b1 one would create a sirp u03b1 sequence database of these individuals stripped of any patient identifiers and perform statistical analysis for association between sirp u03b1 variations and bone marrow engraftment outcomes in the donor host pairs referring to fig9 three snp assays given ds notations were established to detect variable nucleotides underlying several non synonymous coding polymorphisms we detected among the human hapmap samples polymerase chain reaction pcr amplification primers and detection probes were designed based on applied biosystems taqman system the discrimination between sirp u03b1 variants v1 v2 etc is shown at bottom of the table in fig9 fig9 shows the validation of the discrimination power of each snp assay by independent sequencing of the sirp u03b1 igv domain pcr amplified from each of the human hapmap samples shown at the left side of the table the extent of normal human polymorphism in sirp u03b1 igv domain sequence is to be determined and use of both snp assays and sequencing for use in clinical studies is to be optimized the methods and data employed are widely accessible and known by those of ordinary skill in the art see for example the international hapmap project fig1 a is a.