of the present invention is synthesized by standard techniques employing an expression vector containing in its genome the cloned complete coding sequence of ncf anti ncf monoclonal antibodies of the present invention are prepared by standard hybridoma technology and utilized for purification and assaying purposes following standard immunological methodologies well known in the art high performance liquid chromatography hplc in situ hybridization assays northern blotting analyses and the like are typical examples of the standard conventional techniques well known to one of ordinary skill in the art which can be employed for isolation localization differentiation detection or measurement of the mrna for ncf in biological samples it should be noted that the fact that chemically synthesized polypeptide of the present invention at 10 nanomolar concentrations acts as a neutrophil attracting factor is shown by the results in table 1 it may be pointed out that various stimuli cause the release or secretion of more than one chemoattractant without the cdna of the present invention it is clear of course that the presence specifically of the mrna for ncf as an involved factor in a particular clinicopathological situation could not be definitively identified and diagnosed cdna of the present invention due to its binding affinity for mrna for ncf for the first time makes it possible to analyze body samples such as joint fluid sputum alveolar lavage fluid tissue samples and the like to detect the presence or absence of mrna.