for a certain degree of color to appear reaction time and the endotoxin concentration is linear from 0 005 to 50 eu ml the concentration of endotoxin in a sample is calculated from its reaction time by comparison to the reaction time of solutions containing known amounts of endotoxin standard the endotoxin assay method based on kinetic procedures may be used to determine endotoxin in any fluids of pharmaceutical or medical interest where endotoxin determination is currently performed by any other method this includes but is not limited to water for injection water for irrigation lipid emulsions for intravenous feeding aqueous emulsions of vegetable oil salt solutions e g parenterally administered sodium chloride solutions including sodium chloride for injection usp sodium chloride for irrigation usp sodium chloride for inhalation and lactated ringer 39 s solution and blood derivatives e g normal serum albumin plasma protein fraction and anti hemophilic factor usp immune globulin rho d immune globulin and antihuman globulin serum this method may also be used to detect endotoxin in biological fluids where the presence of endotoxin may be diagnostic significance including but not limited to blood lymph urine serum acites fluid lung aspirants and the like all materials coming in contact with the specimen or test reagents must be pyrogen free materials may be.