9 cis r ac all trans r ac or vehicle groups of 48 hr dark adapted mice were analyzed for recovery of dark adaptation after light exposure purified rhodopsin opsin and retinoids in the eye and retinal morphology fig1 c methods mice were exposed to light of intensity 500 cd u00b7 m u2212 2 that bleached u02dc 90 of rhodopsin anesthetized and monitored by erg for one hr to evaluate recovery of dark adaptation rhodopsin was purified and rhodopsin opsin ratios was determined retinoid analyses were performed on dissected eyes removed at 0 10 30 and 60 min after exposure to the same amount of light mice used to evaluate retinal morphology were not exposed to photobleaching prior to analysis rhodopsin purification was done under dim red light as previously deserted zhu l et al j biol chem 279 53828 53839 2004 purified anti rhodopsin c terminus antibody 1d4 mackenzie d arendt a hargrave p mcdowell j h amp molday r s biochemistry 23 6544 6549 1984 was immobilized on cnbr activated sepharose 4b and a 4 6 u00d7 12 mm column was packed with 2 mg of 1 d4 antibody ml of sepharose beads mouse whole eyes were homogenized in 137 mm nacl 5 4 mm na 2 hpo 4 2 7 mm kcl and 1 8 mm kh 2 po 4 ph 7 5 with a glass to glass homogenizer soluble proteins in the supernatant were removed by.