to culture medium continuously still at 30 u03bcm human pluripotent stem cells hpsc were either treated with sc332 y27632 or left untreated before being enzymatically passaged in all experiments untreated cells had an equivalent volume of dmso added as a carrier control with cell survival being assayed 24 hrs post passage hpsc treated with either sc332 or y27632 had average cell survival that was significantly higher than that observed in untreated controls p lt 0 001 fig1 in particular the hesc lines h1 h9 and rc9 and the hipsc nmf ipsc6 had cell survival of 72 u00b1 2 9 73 u00b1 2 8 69 u00b1 1 9 and 74 u00b1 2 6 respectively when treated with sc332 in the same cell lines y27632 resulted in cell survival of 72 u00b1 3 6 73 u00b1 2 1 71 u00b1 1 2 and 74 u00b1 4 0 and dmso alone resulted in survival of 18 u00b1 2 2 18 u00b1 2 4 15 u00b1 3 0 and 19 u00b1 2 4 respectively this result was consistent in both hesc and ipsc data shown is the mean survival u00b1 sem n 3 these results confirm that compound sc332 supports the enzymatic passage of hpsc as effectively as the well established.