that isonicotinoyl chloride hydrochloride was substituted for nicotinoyl chloride hydrochloride 1 h nmr cdcl 3 ppm u03b4 2 47 d 3h 3 15 brs 4h 3 41 brs 2h 3 57 brs 4h 3 85 m 6h 5 42 s 1h 6 50 s 1h 6 91 brs 2h 7 15 brs 2h 7 28 m 2h 8 70 d 2h 3 g of 2 methylthio 6 4 4 morpholino anilino 4 piperazin 1 yl pyrimidine prepared in preparation 2 0 9 ml of ethyl chloroacetate and 1 3 ml of triethylamine were added in sequence to 50 ml of acetonitrile and heated the reaction mixture was stirred at 70 80 u00b0 c for 7 hours then the reaction mixture was slowly cooled to 20 u00b0 c and 30 ml of methanol was added stirred for 1 hour and filtered to give a solid product the product was washed with 15 ml of methanol and dried in vacuo at 35 45 u00b0 c to give 3 41 g of the desired compound 93 yield 1 h nmr dmso d 6 ppm u03b4 1 16 m 3h 2 39 d 3h 3 02 brs 4h 3 15 m 2h 3 35 d 4h 3 44 brs 4h 3 70 d 4h 4 04 m 2h 5 56 d 1h 6 87 m 2h 7 34 d 1h 7 37 d 1h 8 82 s 1h 2 5 g of 2 methylthio.