growth vessel mix and aerate cycle 1 6 hours u2014 run cold tap water for 3 seconds mix and aerate cycle 2 12 hours u2014 run cold tap water for 3 seconds mix and aerate cycle 3 16 hours u2014 run cold tap water for 3 seconds mix and aerate cycle 4 19 hours u2014 run cold tap water for 3 seconds mix and aerate cycle 5 22 hours u2014 run cold tap water for 3 seconds it should be understood that these water injection times can vary based on requirements for specific bacteria ambient temperatures or other conditions affecting dissolved oxygen requirements flow control system 48 which comprises at least one solenoid valve controls the mix aeration cycle as well as the u2018 run tap water for 5 minutes u2019 beginning of cycle which results in the dispensing of the live vegetative bacteria into the desired application this u2018 run tap water for 5 minutes u2019 cycle also replenishes the growth vessel with clean cold tap water just prior to the addition of the solid biological product initiating the preferred 24 hour operational cycle zero jet water inlets 116 118 mounted to or received through apertures in upper support plate 66 are preferably positioned diametrically opposite and equidistant from the geometric center of.