absorption data forthe mixture ofi e coli 0127 b8 50 ppme coli 055 b5 50 ppmbacto s absortus equi 50 ppmlipopolysaccharides enteritidis 50 ppms flexneri 50 ppmwavelength slit width absorb transmittancenm mm ance per cent______________________________________220 0 28 0 390 40 8225 0 24 0 287 51 7230 0 22 0 245 57 0233 0 20 0 250 56 2234 0 20 0 251 56 0235 0 20 0 260 55 0240 0 19 0 297 50 4245 0 18 0 350 44 6250 0 17 0 400 40 0255 0 16 0 416 37 7257 0 16 0 419 38 2258 0 16 0 420 38 0259 0 16 0 419 38 2260 0 16 0 418 38 4263 0 16 0 410 39 0265 0 16 0 400 40 0270 0 16 0 355 44 0275 0 16 0 300 50 0280 0 15 0 240 57 7285 0 15 0 185 65 2290 0 15 0 136 73 8295 0 15 0 088 81 820 ml of each solution were mixed to a final volumeof 1000 ml 4 5 ml of the 50 ppm solution of theabove mixture were diluted to 30 ml the finalconcentration is 7 5 ppm ______________________________________ the absorption maxima for all individual endotoxins are essentially the same i e 259 mm for the five endotoxin mixtures the absorption maxima shifted slightly toward a lower wavelength 258 mm and the peak broadened to some extent from this point on all of the absorption measurements were made at 258 mm in order to examine the linearity of the absorption vs concentration curve a 1 liter solution of the five endotoxins cited above containing a total of 50.