blr de3 the e coli strain xl1 blue transformed with pss5 was deposited in the spanish type culture collection on 29 oct 2003 located in the research building of valencia university burjassot campus burjassot 46100 valencia spain with the deposition number cect 5849 in the present invention ss was overexpressed a constitutively b specifically in leaves and c specifically in storage organs such as tubers for the production of plants that overexpress ss constitutively constructions were created that were controlled by the action of the 35s constitutive promoter of the tobacco mosaic virus successive insertion in pss of the 35s promoter and nos terminator in the 5 u2032 and 31 regions of ssx gave rise to the production of the plasmid p35s ss nos the restriction map of which is shown in fig4 b so as to be able to transfer this construction to the genome of the plants via agrobacterium tumefaciens it must first be cloned in a binary plasmid for this p35s ss nos was digested successively with the enzymes noti t4 dna polymerase and hindiii and was cloned within the binary plasmid pbin20 fig4 a hennegan k p danna k j 1998 pbin20 an improved binary vector for agrobacterium mediated transformation plant mol biol rep 16 129 131 which had previously been digested successively with the enzymes ecori t4 dna polymerase and hindiii the plasmid thus obtained was designated pbin35s ss nos fig4 c to overexpress ss specifically in illuminated leaves pcr was used for amplifying the promoter region designated rbcs of the gene that encodes the small subunit of rubisco ribulose.