of three transgenic lines had 5 to 14 viable pollen rest of the pollens of the plants were not showing fluorescence fig1 on the other hand control plants independent transgenic lines for an expression cassette comprising gus reporter gene driven by tapetum specific promoter showed 80 to 92 pollen viability table 2 fig1 invitro pollen germination test was performed using artificial liquid media proposed by kwack 1964 extensive pollen germination was observed in the cultured pollens of one anther of control plants independent transgenic lines for an expression cassette comprising gus reporter gene driven by tapetum specific promoter however pollens of transgenic lines either failed to germinate or if germinated showed severely retarded pollen tube growth in the transgenic plants independent transgenic lines for the expression cassette comprising plant beclin 1 atg6 gene driven by tapetum specific promoter of four different lines 0 1 pollen germination was observed in comparison to 62 75 in control plants table 1 amp table 2 further pollen grains were observed under scanning electron microscopy the pollen grains of transgenic lines showed difference in sculpturing pattern of exine and lack of germ pores fig3 fruit set was normal in the transgenic plants of all the six lines but the bulbs were of smaller size seed setting was severely affected in bulbs of the transgenic plants seed weight per pod of the transgenic plants of twelve different lines was nil to 32 07 mg whereas in control plants it was 36 6 mg to 113 34 mg table 3 amp table 4 2 it is advantageous to use plant beclin1 atg6 as male sterility gene because it has no product which is cytotoxic outside the target cell 3 the plant beclin1 atg6 as a male sterility dna.