mixed with 36 ml isopropanol 485 ml tap water and 55 ml of the soybean seed hull extract obtained in example i the pulp isopropanol soybean seed hull extract was poured into a jacketed round bottom flask and stirred at 300 rpm at 60 u00b0 c 5 ml of a 15 h 2 o 2 solution and added to the pulp extract solution at 60 u00b0 c and 300 rmp over 5 minutes at the end of the reaction the mixture was filtered on a whatman 4 filter paper by vacuum filtration and the damp pulp was separated from the filter example ii was repeated except that prior to reaction the soybean extract was placed in a 250 ml erlenmeyer flask and heated on a steam bath for 20 minutes to destroy enzymatic activity otherwise the reaction was the same as example ii example ii was repeated using fresh extract but without hydrogen peroxide table 2______________________________________enzymatic deinking of newsprintexample observed color black______________________________________ii light tan 1 2iii boiled enzyme gray black 2iv no peroxide gray black 2______________________________________ sup 1 visual black scale 0 10 a direct assay of peroxidase in the aqueous fraction of each reaction using 1 4 dimethoxybenzene as a substrate indicates that heating reduces the oxidation potential of the peroxidase as shown in table 2 table 3______________________________________peroxidase activity of deinking reactions.