mpnspd on polyamine pools were similar table 2 i e put was depleted below detectable limits and spermidine was reduced to 6 15 of controls while spermine levels were diminished to below 50 dpnspd was not as effective as the other norspermidine analogues in depletion of polyamine pools e g at 500 u03bcm put was only lowered to 68 spd to 71 and no effect on spm level the dipropyl analogue was similar in behavior to the parent norspermidine the corresponding norspermines were again more effective at 100 u03bcm the effect of dmnspm and denspm on polyamine pools was similar i e putrescine was depleted to below detectable limits and spermidine was reduced to around 5 of controls while spermine levels were diminished to 27 36 dmspd and despd at 100 u03bcm depleted put to below detectable limits spd to 5 spm to 58 and 74 of control respectively the monoalkylated spd analogues mespd n 1 mespd n 8 and mpspd n 1 gave a similar pattern of polyamine pool depletion at 100 u03bcm putrescine was depleted to below detectable levels spermidine to 25 and spermine to 80 84 and 90 of control value mpspd n 8 was slightly less active than mpspd n 1 at 500 u03bcm dpspd reduced put to below detectable level and spd to around 10 of control like dpnspd dpspd showed little suppression of spm levels and possibly even some up regulation at 100 u03bcm interestingly at the level of put and spd suppression mpspd n 1 and mpspd n 8 behave very much like their mespd counterparts however the propyl analogues are slightly less effective at spermine suppression the parent amine spd suppresses put but not spd or spm again the corresponding spermines are more effective than the triamines at.