has a low pka it could only be used as an auxiliary component with other mild builders in a detergent formulation since t1 lipase has an optimum ph of 9 0 and stable in between ph 6 0 and 11 0 6 carbonate and bicarbonate were chosen due to their high pka values however the buffering capacity of bicarbonate is only moderate and t1 lipase was greatly destabilized by carbonate fortunately a combination of carbonate glycine at a ratio of 30 70 in an aqueous solution which gave a resulting ph of 9 25 close to the t1 lipase optimum ph at ph 9 0 showed high enzymatic stability table 2 this might indicate that glycine has a stabilizing effect on t1 lipase compensating the effect of the reduction of ca 2 the dishwashing performance of detergent a in ion free water at various temperatures is shown in fig1 as expected the dishwashing performance improved as the temperature increased at 0 ppm of caco 3 a full detergency was almost achievable without the help of t1 lipase the improvement after adding t1 lipase also became smaller after each increment in temperature showing that elevated temperature lowered surface tension of water and promoted better soil removal in addition the dishwashing performance of the formulated detergent was quickly observable in the absence of ionic interference especially at 60 u00b0 c where 50 of soil removal was observed within half of the duration of the test fig2 compares the dishwashing performance of detergent b in hard water of 350 ppm caco 3 at various temperatures similar to the previous results the dishwashing performance improved as the temperature increased but not as much as that in water of 0 ppm caco 3 the performance of the nonionic.