for 1 hour the membrane was washed again with tbst three times 10 min each time and reacted with ecl solution followed by the development on film fig4 illustrates the changes of erk extracellular signal regulated kinase phosphorylation chat choline acetyltransferase i u03bab inhibitor of u03bab nf kappa b nuclear factor kappa light chain enhancer of activated b cells according to the treatment of the fructus mume extract of the present invention fig4 a illustrates the result of western blotting fig4 a illustrates the result of western blotting and fig4 b is a graph illustrating the result presenting the normalization of hippocampal damage normalizing erk phosphorylation and increasing chat by the administration of the fructus mume extract of the present invention fig4 c illustrates the result of western blotting and fig4 d is a graph illustrating the result presenting the normalization of hippocampal damage normalizing nf kappa b by the administration of the fructus mume extract of the present invention the bccao group animals demonstrated similar erk level but higher erk phosphorylation compared with the vehicle treated group animals the treatment of the fructus mume extract reduced erk phosphorylation level significantly p lt 0 05 hippocampal chat choline acetyltransferase the enzyme used for the generation of acetylcholine was reduced in the bccao group animals compared with the vehicle treated group and the reduced chat level.