mm nacl 0 1 gel 0 1 tween 15 ppm kathon ph 7 4 anti chlorpyrifos antibody was prepared by using a chlorpyrifos thyroglobulin porcine immunogen whose structure is str9 wherein the amine moiety on the thyroglobulin is contributed by a primary amine of that protein balb c mice were immunized by injecting them subcutaneously with 200 ug of the chlorpyrifos thyroglobulin immunogen in freund 39 s complete adjuvant three 100 ug subcutaneous injections with freund 39 s incomplete adjuvant followed 3 5 and 6 weeks after initial immunization three weeks after the last injection a 100 ug i p injection was given in phosphate buffer saline mice were sacrificed spleens were removed and their cells fused with peg polyethylene glycol to p3x myeloma cells following screening and recloning appropriate hybridomas were grown as ascites tumors in mice 1 added 200 ul microliters of chlorpyrifos standardizing compound or control 34 diluent zero standard 34 to a test tube 2 added 250 ul of chlorpyrifos hrp conjugate 1 70 000 dilution to the test tube of step 1 3 added 500 ul of magnetic particles coupled to anti chlorpyrifos antibodies to each tube and vortexed the test tube of step 2 4 incubated the test tube for 15 minutes at room temperature about 25 u00b0 c 5 magnetically separated the particles and substances bound thereto from the other contents.