experiment reaction media in all experiments consisted of 1 00 g l glucose 20 00 mg l magnesium sulfate 30 00 mg l dipotassium monohydrogen phosphate and 0 07 mg l vitamin b 12 as required filtered sterilized urea was added in the reaction media to make the urea concentration 100 mg dl plasma from a bovine source was used for the plasma urea and ammonia removal experiments and filter sterilized urea was added to the plasma this was done to mimic the uremic patient 39 s plasma characteristics in vitro two different surgical models were developed and identified as model a and model b in both the models male wister rats with weight range 300 340 g were used the details of the surgical procedure applied to prepare uremia rat models are as follows a two step procedure one to perform right nephrectomy and other to partially ligate the left artery vein and ureter is required the details of the procedure are as follows the anesthetized animal is placed in ventral recumbency with its tail towards the surgeon the hair in the right dorsal lumber area is clipped and the skin swabbed thoroughly with surgical scrub a 2 3 cm incision is made into the skin caudal to the rib cage on the right.