and developed with mixed solvents of chloroform methanol 1 0 19 1 9 1 3 1 1 1 and 0 1 to obtain fractions among these fractions the chloroform methanol 9 1 3 1 fractions were concentrated to obtain 150 2 mg of residue this residue was applied onto four tlc plates merck silica gel 60f 254 20 u00d7 10 cm and developed with a mixed solvent of hexane diethyl ether 1 2 separation was conducted using the uv absorption as an indicator whereby compounds 1 to 6 were isolated in amounts of 109 0 mg 0 5 mg 0 5 mg 1 0 mg 0 5 mg and 2 0 mg respectively physical properties of compounds 1 to 6 thus isolated were as follows the results of nmr spectra are shown in table 1 1 h nmr spectra 400 mhz cd 3 od and table 2 13 c nmr spectra 100 mhz cd 3 od from this information structures of compounds 1 to 6 were determined as follows measured value m z 343 2270 c 22 h 30 o 3 h 4 infrared absorption spectrum u03bb max kbr cm u2212 1 3431 2964 2925 2860 1701 1655 1648 1638 1560 1543 1113 measured value m z 359 2227 c 22 h 30 o 4 h 4 infrared absorption spectrum u03bb max kbr cm u2212 1 3422 2967 2929 2870 1645 1559 1454 1398 1060 measured value m z 359 2224 c 22 h 30 o 4 h 4 infrared absorption spectrum u03bb max kbr cm u2212 1 3421 2968 2923 2869.