cleaned and volunteers are randomly divided into two groups with 30 volunteers each which all the volunteers brush their teeth twice a day in the morning and prior to bed for 3 minutes each time the experimental group uses the igy toothpaste of this invention and the volunteers of control group brush their teeth with classic toothpaste bacterial samples were picked from plaque and tongue surface and inoculated on ts medium as the total anaerobic bacteria medium and tsb medium as a selective medium for s mutans cfu and the percentage s mutans in total anaerobic bacteria was calculated after 36 hrs anaerobic incubation samples were taken once a week for 9 weeks and the results are indicated in table 2 percentage s mutans in total anaerobic bacteria experimental group control group week saliva plaque saliva plaque 0 56 23 56 43 52 56 50 10 1 45 60 42 28 49 10 48 10 2 41 80 38 39 49 20 47 08 3 32 83 30 15 48 10 46 15 4 28 15 19 10 47 70 43 60 5 25 29 18 32 43 19 45 55 6 23 19 17 92 49 20 46 35 7 21 08 15 10 50 05 50 26 8 20 05 13 96 51 16 49 18 for weeks after the starting day the experimental group uses the igy toothpaste in this invention got a deep drop of percentage s mutans in total anaerobic bacteria for both saliva and plaque with statistical analysis result p lt 0 01 reflect that there is significant change before and after experiment on the other hand the control group which uses classic toothpaste remains unchanged statistical analysis.