cross talk u201d between endothelial trophoblast cells causing obvious differences in architecture as compared to the same cells stimulated under the same conditions with nps serum sample l1045 fig2 panel a photograph 1 pre incubation by mixing of recombinant ttr abd serotec purity gt 96 at different concentrations 0 1 1 0 10 u03bcg ml of serum substantially rescues the u201c abnormal endothelial trophoblast cross talk u201d and is shown in fig3 panels d photograph 14 16 amp panel e photograph 18 20 the number of vacuoles or tubes formed per sample were counted and represented in the graph fig4 mpe with and without ttr as compared to the control nps the cells co cultured with mild pe serum exhibited significant decreases in capillary tubes formation 60 versus 20 and 15 statistical significance of experimental differences was assessed using student 39 s paired t test the differences were considered to be statistically significant when the p value was lt 0 05 the in vitro studies conducted in example 2 were quantified by counting the number of tube like structures termed as u201c vacuole u201d under fluorescence microscope nikon eclipse ts 100 coupled with ccd camera in four different fields at 4 u00d7 magnification each vacuole in this context is the small cavity completely bound by elongated cellular structure as indicated in fig2 panel a and fig2 panel c shown by bold arrow as observed under a microscope vacuolization comprises thin walled vacuoles having few branch points the branch points are the points from which multiple vacuoles are initiated connected the quantity of tubes formed also means number of.