resulting admixture was diluted with an equal volume of water and the diluted admixture was extracted three times each time with 250 ml of ether and each time the ether phase was discarded the aqueous phase was made alkaline with 2m naoh and the alkaline admixture was extracted three times each time with 200 ml of chloroform each time the chloroform phase was recovered and the chloroform phases were combined the combined chloroform phases were dried over sodium sulfate and then were evaporated to leave a residue of the azamacrolides in the same relative amounts as occur in the initial drops in one case the capillary tube filled with droplets was used to charge a gas chromatograph and in a second case the residue from the sulfuric acid extraction was used to charge a gas chromatograph and in each case gas chromatography was used to isolate the active constituents in substantially pure form in the gas chromatography methyl silicone was used as the stationery phase and temperature was programmed to increase from 60 u00b0 c to 250 u00b0 c the volatilized material was trapped as it emerged in glass tubes cooled in an ice bath or with dry ice the gas chromatograph column was equipped with a splitter so that 10 of the volatiles were continuously routed to a detector which indicated when volatiles were emerging in both the.