black line in a are artifacts due to the silver staining protocol used nervous fibers dark brown lines invaded the injury site from the rostral side nervous fibers invaded the scaffold both laterally fig7 ab and rostrally fig8 a to the lesion site particularly it was possible to see regenerating nervous fiber extending processes significantly along the longitudinal spinal cord axis fig8 a providing support to the idea of using oriented microfibers to orient nervous regeneration in fig8 b making use of a morphology immuno histochemical staining ematoxilin eosin reaction we colored cytoplasms and cell nuclei the lesion site is artificially bordered by the red curve to note tissue parallel cavities are left by scaffold fibers effect due to the long biodegradation time of 100 esterified ha when implanted in cns tissues to date all the scaffolds whether seeded or not with nscs prevented the formation of aqueous cysts and glial scarring in all of the animal tested the outcome that was found in the injured only animals fig9 ab show a haematoxylin eosin and b neurofilament stainings of consecutive longitudinal sections of injured spinal cord control group the animal was sacrificed 6 months after surgery u201c r u201d points to the rostral side of the spinal cord left.