d 150 ecu g fibres and incubated at 50 u00b0 c for 2 hours the samples were mixed manually every 30 minutes then the samples were washed with de ionized water and the enzymes were then denaturated at 80 u00b0 c for 30 minutes at the end the pulp sample was washed with de ionized water again 3 the pre treated pulps were refined once again with the escher wyss refiner to u00b0 sr values shopper riegler between 90 and 95 average refining energy 90 kwh tonne specific edge load 1 ws m 4 subsequently the material was passed through a high pressure fluidizer homogenizer microfluidizer m 110eh microfluidics corp the 2 w w concentration pulp fibre slurry was passed through two differently sized chamber pairs each pair connected in series first the slurry passed three times through a chamber pair with a diameter of 400 u03bcm and 200 u03bcm the first chamber and the second chamber respectively and then 5 times through a chamber pair with a diameter of 200 u03bcm and 100 u03bcm the operating pressures were 105 mpa and 170 mpa respectively the material was also produced using different chambers and different number of passes through the chambers showing that if the pre treatment was done in a good fashion these parameters chamber type and number of passes did essentially not matter two cases were tried cases e and f in both these cases the production method was done according to case c with the exception of the choice of chambers and the number of passes in case e the material was passed one time through a chamber pair with a diameter of 200.