the biofuel according to the invention is an alcohol for example ethanol butanol and or isopropanol more preferentially the biofuel according to the invention is ethanol in another embodiment the product is a molecule of interest to the chemical industry for instance another alcohol such as 1 2 propanediol 1 3 propanediol 1 4 butanediol 2 3 butanediol organic acids such as acetic acid propionic acid acrylic acid butyric acid succinic acid malic acid fumaric acid citric acid or itaconic acid or hydroxy acids such as glycolic acid hydroxypropionic acid or lactic acid described below is an embodiment of production of an enzymatic cocktail that is useful for the hydrolysis of lignocellulose the strains of filamentous fungi preferably trichoderma more preferentially t reesei capable of expressing at least one polypeptide according to the invention are cultured in fermentors in the presence of a carbon based substrate such as lactose or glucose chosen for growth of the microorganism in one embodiment this carbon based substrate depending on its nature is introduced into the fermentor before sterilization or is sterilized separately and introduced into the fermentor after sterilization of the latter so as to obtain an initial concentration of 20 to 35 g l an aqueous solution containing the substrate chosen for the production of the enzymes is then added an enzymatic composition which acts on the lignocellulosic biomass produced by the fungi is finally recovered by filtration of the culture medium in this composition are in particular the u03b2 glucosidase the exoglucanase and the endoglucanase according to the invention in one embodiment the aqueous solution containing the substrate chosen for the production of the enzymes is prepared at the concentration of 200 250 g l this solution also preferably contains an inducer substrate.