refers to a test run with lithium formulated buffer 34 lin 34 refers to a test run with lithium formulated buffer containing 10 u03bcm nicotine 34 dtc 34 refers to a test run with lithium formulated buffer containing 10 u03bcm nicotine and 10 mm d tubocurarine 34 mec 34 refers to a test run with lithium formulated buffer containing 10 u03bcm nicotine and 10 u03bcm mecamylamine 34 stx 34 refers to a test run with lithium formulated buffer containing 10 u03bcm nicotine and 5 nm neosurogatoxin 34 u03b1bt 34 refers to a test run with lithium formulated buffer containing 10 u03bcm nicotine and 100 nm alpha bungarotoxin and 34 kbt 34 refers to a test run with lithium formulated buffer containing 10 u03bcm nicotine and 100 nm kappa bungarotoxin the data are normalized to the control value na of 100 transfected cells were cultured in 35 mm tissue culture dishes as described above to enhance expression of nachr subunits see leutje et al supra 10 mm sodium butyrate claudio et al science 238 1688 1694 1987 was added to the culture medium 48 hours prior to recording at this time cells which had become rounded with a spider like morphology were tested for their response to acetylcholine recordings were obtained on cells at room temperature using the whole cell patch clamp configuration and a daga 8900 patch clamp amplifier 0 1 g ohm probe pipettes were made from borosilicate glass dagen corp le 16 and coated within 100 u03bcm of the tip with sylgard the initial resistance of fire polished pipettes ranged from 4 to 8 megaohms the patch pipette contained 133 mm kcl 1 mm cacl 2 and 10 mm hepes ph 7 3 control bath solution.