but also appeared to have some beef and pork components fig5 a d lane 8 the mixed sausage contained beef and pork as labeled and also trace amounts of chicken fig5 a d lane 9 quantitative pcr analysis was undertaken for six meat samples using the same primers and sybr green fluorescence detection results are shown in fig6 in fig6 a through 6d the six products were labeled as follows a ground beef u2014 open triangles b ground pork u2014 open squares c ground lamb u2014 open circles d pork sausage u2014 filled triangles e chicken sausage u2014 filled squares f mixed beef and pork sausage u2014 filled circles the pcr cycle at which the fluorescent signal crosses baseline is considered to be the threshold cycle plotted on the y axis the fluorescent signal produced by a 10 fold dilution series of a bovine b porcine c chicken or d bovine and ovine dna is plotted as the mean of duplicates u00b1 1 standard deviation to form a standard curve dna 10 ng from each meat sample was analyzed in duplicate using each of the four quantitative assays values were calculated using the standard curves and plotted as the mean with x and y error bars equal to 1 standard deviation values significantly different from the no template control are marked with an asterisk p 0 05 the results from example 2 indicated that both the ground beef sample and the ground lamb sample contained trace amounts of pork 0 17 u00b1 0 10 pg u02dc 0 002 and 0 40 u00b1 0 00 pg u02dc 0 004 respectively see fig6 b these calculated values.