u00b0 37 u00b0 c and continuously shaken at 60 80 strokes per minute the incubation lasts for about 20 30 hours the broth from the cw charcoal culture 2 5 is transferred to a bottle containing 3 liters of modified cw or modified ss medium with 3 g of dowex u00ae 1 x8 resin or the like and with between 0 5 mg ml and 6 0 mg ml of u03b2 lactoglobulin this culture is incubated at 35 u00b0 37 u00b0 c while continuously being shaken at 60 80 strokes per minute the incubation lasts for about 20 30 hours the culture is used to innoculate a fermentation tank a fermentation tank containing approximately 300 liters of distilled water is sanitized for a minimum of one hour at 120 u00b0 123 u00b0 c once cooled it is drained charged with 400 g of dowex u00ae 1 x8 resin or the like and 400 liters of the modified cw or modified ss medium is pumped into the tank and sterilized for 15 35 minutes at 120 u00b0 123 u00b0 c the tank is cooled to 32 u00b0 38 u00b0 c and held overnight under 10 lbs sterile air pressure a sterile filtered solution of u03b2 lactoglobulin is added to the tank medium to arrive at a final concentration of 0 5 6 0 mg ml the culture from the liquid medium is inoculated into the fermentation tank and allowed to grow for about 20 48 hours at 32 38 u00b0 c with agitation and with approximately.