in order to facilitate infection and the positive control in which only conidia of the fungus inoculated suffered an infection by botrytis cinerea that covered practically 100 of the surface of the leaves both in the leaves protected by paenibacillus polymyxa schc33 and in those protected by bacillus subtilis qst 713 there were significant decreases in the degree of damage caused by the fungus in the case of paenibacillus polymyxa schc33 using a ratio of conidia bacteria of 1 10 the damage produced by the fungus only covered 20 5 of the leaf surface decreasing to 11 7 when the ratio was 1 100 in the trials with bacillus subtilis qst 713 plant tissue necrosis produced by the fungus covered 36 9 of the leaf surface for a ratio of 1 10 and decreased to 15 4 when the ratio was of 1 100 conidia bacteria respectively these results are shown in table 3 and fig5 showing that the bacterium has a great potential to be used as biofungicide by direct inoculation in the field of vegetables susceptible to be infected by botrytis cinerea protection bioassays against b cinerea were carried out on clusters of grapes of the thompson seedless variety using 2 known amounts of bacteria 10 7 and 10 8 cfu ml to evaluate their efficiency as a biocontrol agent the results at 30 days were of a protection comparable to that shown in vines leaves since the clusters inoculated with the bacteria by spray did not present evident growth of mycelium of b cinerea for both amounts of bacteria used the negative controls did not show b cinerea presence either in clusters inoculated with only bacteria 10 8 cfu ml or in those inoculated with sterile water alone in addition these results are clear evidence of the.