the incubation chamber 23 and the detection chamber 24 to facilitate the color development after the developing step the second disc 12 will be rotated at still another of the at least one release speed at this rotational speed the centrifugal force applied on the second magnetic units 1311 is greater than the magnetic force and the reagent i in the first main chamber 123 will surmount the first microvalve 1231 the second microvalve 1251 and the third microvalve 1271 and flow into the detection chamber 24 of the reaction unit 2 to inactive reactions and generate end products in the embodiments of fig7 the release speeds for the reagent i the reagent ii and the reagent iii are different and each is greater than another that is the release speed for the reagent i is greater than that of the reagent ii while the release speed for the reagent ii is greater than that of the reagent iii in the analyzing step the second disc 12 will be rotated at an analysis speed which is yet another rotational speed while under the detection of a spectrophotometer the spectrophotometer is used to obtain the optical density of each end product the embodiments of fig7 are used to explain the underlying mechanism of the centrifuge magnet based analyzers though the following embodiments are also based on elisa and the second disc 12 and the third disc 13 in fig7 are collectively referred to as the u201c combined discs u201d therein it should be obvious that the application of the centrifuge magnet based analyzers is not limit to elisa the first experiment was conducted to determine the concentration of human chorionic gonadotropin hcg in.