in 10 dmso and the mixture was incubated for 10 min at rt the kinase reaction was initiated by the addition of 10 u03bcl 4 u00d7 substrate cocktail containing the dye peptide substrate 0 or 5 u03bcm atp 20 u03bcm and 33 p u03b3atp 2 u03bcci rxn after incubation at 30 u00b0 c for 15 min the reaction was terminated by the transfer of 25 u03bcl pf the reaction sample to a 96 well 0 65 u03bcm millipore madvnob membrane plate containing 200 u03bcl 5 mm edta and 20 streptavidine coated beads in pbs the unbound radionucleotides were washed under vacuum with 3 u00d7 250 u03bcl 2m nacl 2 u00d7 250 u03bcl 2m nacl 1 phosphoric acid 1 u00d7 250 u03bcl h 2 o after the last wash membrane plates were transferred to an adaptor plate heat dried for 15 min at 60 u00b0 c and 50 u03bcl scintillation cocktail was added to each well and 4 h later the amount of radioactivity was counted in a top counter the percent inhibition was calculated based on the uninhibited enzyme rate inhibition 100 1 ic 50 inhibitor conc n the ic 50 was calculated using a non linear curve fit with xlfit software id business solution ltd guilford surrey uk the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity and understanding it will be obvious to one of skill in the art that changes and modifications may be practiced within the scope of the appended claims therefore it is to be understood that the above.