his klenow batch 1012 pcr plates 96 welll millipore nah 2 po 4 merck nacl merck imidazol usb the inventive apparatus pyrosequencing phast electrophoresis equipment amersham biosciences phast 10 u2013 15 gels amersham biosciences sds buffer strips amersham biosciences low molecular weight amersham biosciences calibration kit commassie brilliant blue stain amersham biosciences phast silver stain kit amersham biosciences edta merck deionized water nicl 2 x6 h 2 o sigma binding and wash buffer 10 mm nap i 0 5 m nacl 10 mm imidazol ph 7 4 elution buffer 10 mm nap i 0 5 m nacl 0 2 m imidazol ph 7 4 the gel was prepared according to instructions supplied by the manufacturer 1 the column was washed with 50 mm edta 2 ml 2 wash with water 5 ml 3 charging with 0 1 m nicl 2 2 ml 4 wash with water 5 ml 5 wash with binding buffer 5 ml subsequently the column was opened the gel recovered and made to 50 slurry by addition of binding buffer 1 25 u03bcl lysate was dispensed to 7 wells in a pcr plate column a 3 10 5 4 3 2 1 and 0 5 u03bcl gelslurry was added to well a g respectively 4 the plate was incubated for 5 min on a shaker 5 the inventive apparatus was used to aspirate the gel from the used wells 6 the gel was washed for 10 20 s by transfering the inventive apparatus to through containing binding buffer 7 bound proteins were eluted by transfering the inventive apparatus to a fresh pcr plate containing 50 u03bcl elution buffer 8 a sample was withdrawn from each well for analysis a sample of the starting material i e lysate was also taken 9.