the space below the membrane 62 that comprises a second compartment depending upon the particular test or experiment being conducted the same or a different solution m 2 may be disposed within the support 42 and in contact with the tissue sample t and the upper surface of the membrane 62 the fill height of the separate solutions m 1 and m 2 in the annular space 74 and within the support 42 the separate compartments may or may not be the same in order to promote the growth of a monolayer of cells on the upper surface of the membrane within the support 42 as suggested in fig2 the support 42 and the membrane may be treated by corona discharge or other technique so as to reduce surface tension of the surfaces when so treated the tissue sample t and the tissue culture cultivated in the system will attach firmly to the membrane and seal at the edges 80 of the membrane 62 where the membrane joins the radius 66 of lower end 60 of the support in fig2 a thin monolayer of tissue cells t 2 is suggested extending to the edges of the membrane during the growth of the tissue culture the lid 26 of the culture plate is placed over the base as suggested in fig1 and when desired the lid may be removed and a pipette inserted as shown in fig2 to either remove or add medium to the space 74 the tissue culture will receive a substantial portion of its nutrients required for growth through the permeable.