conducted every 12 hours pl 18 seq id no 1 freeze dried powder injection supplied by jiangsu protelight pharmaceutical amp biotechnology co ltd levofloxacin hydrochloride and sodium chloride injection supplied by yangtze river pharmaceutical group and 0 9 sodium chloride injection supplied by chifeng rongjitang pharmaceutical co ltd 1 the preparation of bacterial suspension is the same as above 2 the candida albicans suspension cultured overnight is diluted to 1 u00d7 10 6 cfu ml with sodium chloride and 5 u00d7 10 5 cfu of which is injected into abdominal cavity of every mouse the escherichia suspension cultured overnight is diluted to 2 u00d7 10 8 cfu ml and 1 u00d7 10 7 cfu of which is injected into abdominal cavity of every mouse to make model with mixed infections 3 the blank control group is injected with normal saline into their abdominal cavity the positive control group is injected with levofloxacin into their abdominal cavity 4 administration the drugs are administered according to weight right after the model is ready all the groups except the blank and the infected negative control group are injected with different dosage of different injections through caudal vena twice a day successively for three days and they are observed for fourteen days 5 observe death of the animals and record their survival time experimental result of pl 18 seq id no 1 injection takes on survival time of the mice with multiple mixed infection of candida albicans and escherichia table 16 experimental.