hybridization was as follows u201c rapid hyb buffer u201d amersham life science was used and after prehybridization at 68 u00b0 c for 30 min the labeled probes were added and the solution was incubated at 68 u00b0 c for 2 hours to perform hybridization then washing was conducted 3 times in 2 u00d7 ssc 0 01 sds at room temperature for 20 min then 3 times in 1 u00d7 ssc 0 1 sds at 37 u00b0 c for 20 min and twice in 1 u00d7 ssc 0 1 sds at 50 u00b0 c for 20 min detection was performed by autoradiography northern blot analysis showed that the 4 4 kb and 2 5 kb bands were weakly expressed in most tissues in human except for testis ovary and placenta in contrast in testis ovary and placenta very strong expression was observed fig4 higher expression of mrna in human cultured cells was observed than in tissues moreover in human normal cultured cells the expression amount of the 2 5 kb mrna was nearly 10 times as high as that for the mrna of 4 4 kb fig5 in mouse the 3 5 kb and 2 5 kb bands were weakly expressed in most tissues except for brain spleen and testis no expression was observed in brain or spleen and in testis only the 2 5 kb band was expressed in testis and ovary only the short type of gros1 mrnas was detected it was shown.