vials as shown in fig3 left vial the vials containing the medium with c albicans were incubated at 20 25 u00b0 c for one week and then 30 35 u00b0 c for a second week after two weeks of incubation the vials were visually observed for detection of growth of c albicans vials having headspace showed positive growth of c albicans as shown in fig3 left vial indicated in the photograph as u201c positive growth u201d vials filled by methods herein were found to have been filled with no headspace following incubation the c albicans cells were found to have showed no candida albicans growth as shown in fig3 right vial indicated in the photograph as u201c no growth u201d a trypticase soy broth solution that supports microbial growth was prepared and an inoculum of hyphal fungus a niger was added to the medium the medium containing the a niger was filled into vials a portion of the vials were filled using the process of the present invention no headspace no gas bubble was observed in an exemplary vial as shown in fig4 right vial control vials were filled using a different method production of a large headspace presence of a gas bubble was observed in the control vials as shown in fig4 left vial the vials containing the medium with a niger were incubated at 20.