c especially at least 75 u00b0 c molecules to which the oligonucleotide probe hybridizes under these conditions are detected using an x ray film the present invention also includes lipolytic enzymes and nucleotide sequences encoding same that have homology to the disclosed sequences more preferably the lipolytic enzymes and the nucleotide sequences of the invention may have homologies to the disclosed sequences of at least 70 at least 80 at least 85 at least 90 or at least 95 e g at least 96 at least 97 at least 98 for purposes of the present invention alignments of sequences and calculation of homology scores were done using a needleman wunsch alignment i e global alignment useful for both protein and dna alignments the default scoring matrices blosum50 and the identity matrix are used for protein and dna alignments respectively the penalty for the first residue in a gap is u2212 12 for proteins and u2212 16 for dna while the penalty for additional residues in a gap is u2212 2 for proteins and u2212 4 for dna alignment is from the fasta package version v20u6 w r pearson and d j lipman 1988 u201c improved tools for biological sequence analysis u201d pnas 85 2444 2448 and w r pearson 1990 u201c rapid and sensitive sequence comparison with fastp and fasta u201d methods in enzymology 183 63 98 a substrate for lipase is prepared an emulsion of 5 by volume of tributyrin glycerin tributyrate.