followed by installation of a polycarbonate bladder chamber 120 on the tip tilt plate 115 e g procedure 1720 in fig1 the exemplary tissue mounting device 100 for fresh tissue 105 shown in fig1 can have no force applied to the bladder 125 under this nominal condition the tissue surface e g a lower surface of tissue 105 which can be imaged may not be flattened on to the object plane fig2 shows a side cross sectional view of the exemplary tissue mounting device 100 of fig1 with an inserted piston 205 during operation thereof in particular as shown in fig4 which illustrates a perspective isometric e g 3d view of the exemplary tissue mounting device 100 of fig1 with separate exemplary components illustrated therein a polycarbonate piston 205 can be inserted into the bladder chamber 120 of the tissue mounting device 100 of fig1 e g procedure 1725 in fig1 and pressed down gently to apply pressure on the bladder 125 e g procedure 1730 in fig1 this operation and force can flatten a lower surface of the tissue 105 against the window 110 and thus provide the tissue 105 into a desired 2d plane the exemplary system apparatus which can be used to facilitate such flattening of the tissue is illustrated in fig4 which can facilitate the use of a microscope to analyze the flattened surface of the tissue according to an exemplary embodiment of the present disclosure position and orientation.