hpv16 l2 epitope 14 37 amino acids generated seq id no 5 encoding seq id no 6 when ligated to the 5 u2032 end of hev gene and seq id no 7 encoding seq id no 8 when it was ligated to the 3 u2032 end of the of the hev orf2 pcr fragment the ligation was achieved with a bamh1 site located at either end of the primers for hev or hpv l2 sequence the following primers were used for amplification of 14 37 amino acids of the hpv16 l2 sequence for fusion at the n terminus of the hev orf2 fragment 5 u2032 atgtcgaattcaccatggctgcaactcagttgtataaaac 3 u2032 and 5 u2032 atcgaggatccttgaacctttgggataatgtc 3 u2032 for amplification and fusion at the c terminus of the hev orf2 fragment the following primers were used for hpv16 l2 pcr of the above region 5 u2032 atgtcggatcctctgcaactcagttgtataaaac 3 u2032 and 5 u2032 atcgagcggccgctcattaaccttcaacctttgggataatgtc 3 u2032 pcr primers that were designed to amplify the 5 u2032 ttggttgaagaaacctcctttattgacgctggtgctcca 3 u2032 region of hpv16 l2 gene corresponding to the protein sequence lveetsfidagap also contained a bamh1 site for ligation of the two gene fragments chimeric fusion of the gene fragment encoding lveetsfidagap to the c terminus of hev orf2 resulted in seq id no 9.