a nut is 5 3 grams harvest the nuts are generally ready for harvest between september 30 october 10 no special conditions are needed for harvesting the nuts are simply picked up by hand as they mature and fall to the ground naturally once harvested the nuts are counted and the husks removed the nuts are weighed and then air dried disease resistance the tree retains its leaves late into the growing season and appears to be resistant to many foliar diseases isoelectric focusing isoelectric focusing was conducted on the parent tree of this invention as well as the parent tree of hpc 120 u s ser no 08 618 280 and stw 13 u s ser no 08 617 625 by isolab inc akron ohio to develop an enzyme profile for these trees the procedure used is described below sample preparation ten individual leaves from the parent tree was homogenized with an enzyme extraction solution the samples were allowed to incubate at 4 u00b0 8 u00b0 c overnight the samples were then centrifuged and the resulting supernant was ready for electrophoresis in a ph gradient isoelectric focusing the supernant was run through a broad ranged ph 3 10 hypure gel isolab inc akron.