in two step immunochromatography commercial oil based ink correction fluid made by zebra co ltd is applied in various patterns around the reaction site on the aforesaid chromatography strips a see fig2 and after drying the same mixtures of liquid specimen and colloidal gold marker antibodies as in 9 above are allowed to impregnate the strips and after 5 minutes of development the 34 34 or 34 34 indicators in the reaction sites are compared visually the results of the indicator appearances and shapes are illustrated in fig2 11 study of notches for 34 34 and 34 34 indicators in one step immunochromatography various notches are cut as in 9 above on the aforesaid immunochromatography strips b see fig3 and 500 u03bcl portions of each of the hcg containing specimens as in 9 above or a blank of pbs containing 0 1 wt bsa in test tubes are allowed to impregnate only the lower 5 mm of the strips and after 5 minutes of development the 34 34 or 34 34 indicators in the reaction sites are compared visually the results of the indicator appearances and shapes are illustrated fig3 12 study of applying oil based ink for 34 34 and 34 34 indicators in one step immunochromatography commercial oil based ink is.