be added to a reaction mixture alone or in combination with each other as appropriate a surfactant to be used may for example be sodium dodecylsulfate polyethylene glycol mono p isooctylphenylether cetylpyridinium bromide and the like and a coenzyme may for example be nicotinamideadenine dinucleotide nicotinamideadenine dinucleotide phosphate adenosine 5 u2032 phosphate flavine mononucleotide pyridoxal phosphate and coenzyme a and the like a metal salt may for example be monopotassium dihydrogen phosphate disodium monohydrogen phosphate magnesium sulfate heptahydrate ferrous sulfate heptahydrate zinc sulfate heptahydrate manganese sulfate trihydrate cobalt chloride hexahydrate and the like and a trace nutrition may for example be a yeast extract an organic solvent may for example be an alkane such as n heptane cyclohexane and isooctane an ether such as methyl tert butylether an alcohol such as methanol isopropanol and n octanol a sulfoxide such as dmso a ketone such as acetone a keto acid such as oxaloacetic acid pyruvic acid and u03b1 ketobutyric acid an alkaline metal salt of a keto acid such as sodium pyruvate an alkylester of a keto acid such as methyl pyruvate the other of the optical isomers optical isomer ii i e an l or d form of the amino acid 1 thus produced may be recovered from a reaction mixture by a known method for example the biological material of the present invention is separated from a reaction mixture by a centrifugation or an equivalent method to obtain a supernatant which are then applied to methods like ion exchange chromotography to yield the amino acid or which is then made acidic and extracted with an organic solvent such as diethylether and toluene to remove an organic layer and then an aqueous layer is made basic and extracted similarly with an organic solvent to remove an.