detection methods in other words in one embodiment since the aptamer can be used to replace the skeletal troponin i antibody the detection method for the skeletal troponin i protein in the invention can be any detection method adopting the binding principles of the skeletal troponin i antibody and antigen for example the skeletal troponin i protein is coupled on the microspheres via 1 ethyl 3 3 dimethylaminopropyl carbodiimide edc the aptamer is then incubated with skeletal troponin i coated beads in phosphate buffered saline pbs the aptamer bead complex will be formed after one hour incubation at room temperature in order to detect the presence of the complex the aptamer bead complex is incubated with the strepavidin phycoerythrin conjugates and the fluorescence signal which is expressed in term of mean fluorescent intensity mfi is detected using the luminex 200 3 1 xponent system the invention is directed to another detection method for the skeletal troponin i protein the detection method is suitable for detecting the skeletal troponin i protein in a sample and includes the following a plurality of beads is provided and the beads are covalently bonded with the skeletal troponin i protein the beads and the sample are.