u2014 ortho so 3 h another suitable column for use in the methods of the invention is the prometic biociences blue sa column which is one of a family of dye ligand columns having the resin structure shown in fig1 in one aspect the dye ligand column is a variant of the cibacron blue 3ga column e g a column wherein the r group is not a mixture but is all u2014 nh u2014 c 6 h 4 u2014 meta so 3 h or u2014 nh u2014 c 6 h 4 u2014 ortho so 3 h preferably the synthetic chemical resin has an affinity for hsa that is at least 2 5 10 20 50 or even 100 fold greater than its affinity for other serum albumins e g non human mammalian serum albumins e g bsa loading buffers appropriate for loading a sample containing hsa onto a column will depend upon the specific column in addition those skilled in the art will recognize that there are many different buffers suitable for loading an hsa sample onto any particular column a preferred loading buffer for the prometic biosciences blue sa column includes e g 50 to 250 mm salt e g nacl or kcl at ph 8 9 and a low concentration of a non ionic detergent e g 0 01 to 0 1 polysorbate 20 i e tween 20 a clarified hsa sample is preferably diafiltered prior to being loading onto a column in order to exchange the buffer of the clarified hsa sample for an appropriate column loading buffer a clarified hsa sample can also be filtered so.