The victim was trying to cross the A590 near the Heaves Hotel, Levens, near Kendal just after 19:30 GMT, Cumbria Police confirmed.
A spokesman for the force said the woman, who was in the east-bound lane, was pronounced dead at the scene.
Police are appealing for anyone who was in the area who may have witnessed the accident to come forward.
A woman killed crossing a road in the Lake District may have been struck by several vehicles, police have said.