messages.content: 请把下面的中文句子翻译成英文
messages.role: system
messages.content: 使用PGE2和前列腺素E受体(prostaglandin E receptor，EP)激动剂 、RNAi技术下调巨噬细胞EP4受体表达、抑制性表达DN.CREB质粒处理LPS诱导的小鼠腹腔巨噬细胞。并通过Western印迹测定HMGB1表达水平。
messages.role: user
messages.content: The PGE2, prostaglandin E receptor (EP) 4 agonist, EP4 RNAi, and DN.CREB inhibitory plasmid were used to interfere with the LPS-treated mouse peritoneal macrophage.
messages.role: assistant