Next, by using miRwalk 2.0 and TargetScan version 7.1, the microRNAs that target critical genes in the PI3k/AKT pathway were selected according to score. 
miRwalk
Next, by using miRwalk 2.0 and TargetScan version 7.1, the microRNAs that target critical genes in the PI3k/AKT pathway were selected according to score. 
TargetScan
The molecular features of glioblastoma were studied using KEGG and TCGA sites. 
KEGG
The molecular features of glioblastoma were studied using KEGG and TCGA sites. 
TCGA
The expression levels of miR-548x, miR-4698 and target genes were detected by qRT-PCR. 
qRT-PCR
The MTT, cell cycle, annexin and colony formation assay was used to detect the cell proliferation. 
colony formation assay
MiR-548x and miR-4698 predicted target genes (Rheb, AKT1, mTOR, PDK1) were also evaluated by luciferase assay. 
luciferase assay
The expression of AKT was detected by western blotting. 
western blotting