Serum and CP were collected and subjected to RT-qPCR analysis and western blots. 
RT-qPCR
Serum and CP were collected and subjected to RT-qPCR analysis and western blots. 
western blots
Serum was used to measure steroid levels using liquid chromatography tandem mass spectrometry (LC-MS/MS).



liquid chromatography tandem mass spectrometry (LC-MS/MS)