Improved in vitro assay of resistant starch in cross-linked phosphorylated starch.In the AOAC Method 2002.02 assay for resistant starch (RS), the enzyme-resistant starch is solubilized by cold 2 M KOH, so that it can be converted enzymatically to glucose. 
AOAC Method 2002.02
1) the directions in AOAC Method 996.11for total starch assay using hot DMSO or cold KOH; 
AOAC Method 996.11
2) two back-to-back incubations with thermal-stable α-amylase in a boiling water bath; and  3) a KOH treatment followed by two back-to-back incubations with thermal-stable α-amylase in a boiling water bath. 
thermal-stable α-amylase