A Single-Cell RNA-Seq-Based Approach for Genome-Wide Identification of Cell Essential Genes.Identification of genes essential for structure, function, and survival of a cell type is critical for understanding of the underlying mechanisms.  In this approach, mouse podocytes are subjected to ultra-deep single-cell RNA-seq, and the genes expressed in all single podocytes are sorted out and considered as the candidates of podocyte essential genes. 
RNA-Seq
Studies by single-cell RNA sequencing have shown that gene expressions of single cells of the same type are highly heterogeneous. 
single-cell RNA sequencing
In this approach, mouse podocytes are subjected to ultra-deep single-cell RNA-seq, and the genes expressed in all single podocytes are sorted out and considered as the candidates of podocyte essential genes. 
ultra-deep single-cell RNA-seq