Electrospun PLGA and PLGA-PEG nanofibers were fabricated and physicochemical properties were examined using the HNMR technique. 
HNMR
Scaffolds' features, cell morphology, attachment, and alignment were monitored by SEM imaging.  According to SEM analysis, the PLGA-PEG scaffold had less thick nanofibers compared with the PLGA scaffold. 
SEM
We performed MTT assay to measure cell survival rate.  MTT assay revealed that both scaffolds did not exert any neurotoxic effects on cell viability. 
MTT assay
To evaluate neurite formation and axonal outgrowth, cells were stained with an antibody against β-tubulin III using immunofluorescence imaging. 
Immunofluorescence imaging