Specific PCR-based detection of Phomopsis heveicola the cause of leaf blight of Coffee (Coffea arabica L.) in China.Coffee (Coffea arabica L.) is currently grown in many tropical and subtropical areas countries and is a major traded commodity for the developing world.  This study aimed to develop a PCR-based diagnostic method for detecting P. heveicola in planta.  The efficiency and specificity of CPHF/CPHR were established by PCR analysis of DNA from P. heveicola strains isolated from China and fungal isolates of other genera.  The specific primers designed and employed in PCR detected P. heveicola up to 3 pg from DNA isolated.  This is the first report on the development of a species-specific PCR assay for identification and detection of P. heveicola.  Thus, the PCR-based assay developed was very specific, rapid and sensitive tool for the detection of pathogen P. heveicola.
PCR
Specific PCR-based detection of Phomopsis heveicola the cause of leaf blight of Coffee (Coffea arabica L.) in China.Coffee (Coffea arabica L.) is currently grown in many tropical and subtropical areas countries and is a major traded commodity for the developing world.  This study aimed to develop a PCR-based diagnostic method for detecting P. heveicola in planta.  The efficiency and specificity of CPHF/CPHR were established by PCR analysis of DNA from P. heveicola strains isolated from China and fungal isolates of other genera.  The specific primers designed and employed in PCR detected P. heveicola up to 3 pg from DNA isolated.  This is the first report on the development of a species-specific PCR assay for identification and detection of P. heveicola.  Thus, the PCR-based assay developed was very specific, rapid and sensitive tool for the detection of pathogen P. heveicola.
PCR
Specific PCR-based detection of Phomopsis heveicola the cause of leaf blight of Coffee (Coffea arabica L.) in China.Coffee (Coffea arabica L.) is currently grown in many tropical and subtropical areas countries and is a major traded commodity for the developing world.  This study aimed to develop a PCR-based diagnostic method for detecting P. heveicola in planta.  The efficiency and specificity of CPHF/CPHR were established by PCR analysis of DNA from P. heveicola strains isolated from China and fungal isolates of other genera.  The specific primers designed and employed in PCR detected P. heveicola up to 3 pg from DNA isolated.  This is the first report on the development of a species-specific PCR assay for identification and detection of P. heveicola.  Thus, the PCR-based assay developed was very specific, rapid and sensitive tool for the detection of pathogen P. heveicola.
PCR
Specific PCR-based detection of Phomopsis heveicola the cause of leaf blight of Coffee (Coffea arabica L.) in China.Coffee (Coffea arabica L.) is currently grown in many tropical and subtropical areas countries and is a major traded commodity for the developing world.  This study aimed to develop a PCR-based diagnostic method for detecting P. heveicola in planta.  The efficiency and specificity of CPHF/CPHR were established by PCR analysis of DNA from P. heveicola strains isolated from China and fungal isolates of other genera.  The specific primers designed and employed in PCR detected P. heveicola up to 3 pg from DNA isolated.  This is the first report on the development of a species-specific PCR assay for identification and detection of P. heveicola.  Thus, the PCR-based assay developed was very specific, rapid and sensitive tool for the detection of pathogen P. heveicola.
PCR
Specific PCR-based detection of Phomopsis heveicola the cause of leaf blight of Coffee (Coffea arabica L.) in China.Coffee (Coffea arabica L.) is currently grown in many tropical and subtropical areas countries and is a major traded commodity for the developing world.  This study aimed to develop a PCR-based diagnostic method for detecting P. heveicola in planta.  The efficiency and specificity of CPHF/CPHR were established by PCR analysis of DNA from P. heveicola strains isolated from China and fungal isolates of other genera.  The specific primers designed and employed in PCR detected P. heveicola up to 3 pg from DNA isolated.  This is the first report on the development of a species-specific PCR assay for identification and detection of P. heveicola.  Thus, the PCR-based assay developed was very specific, rapid and sensitive tool for the detection of pathogen P. heveicola.
PCR