CRISPR loci-PCR as Tool for Tracking Azospirillum sp.  In this context, clustered regularly interspaced short palindromic repeats (CRISPR) loci structure is unique in the bacterial genome, including some Azospirillum species.  Here, we investigated the use of CRISPR loci to track specific Azospirillum strains in soils systems by PCR.  The CRISPRloci-PCR approach was standardized for Azospirillum sp.  The CRISPRloci-PCR approach was validated in assays with substrate and wheat seedlings.  The CRISPRloci-PCR approach was found to be a useful molecular tool for specific tracking of Azospirillum at the strain level.  This technique can be easily adapted to other microbial inoculants carrying CRISPR loci and can be used to complement other microbiological techniques.
CRISPR
CRISPR loci-PCR as Tool for Tracking Azospirillum sp.  Here, we investigated the use of CRISPR loci to track specific Azospirillum strains in soils systems by PCR.  strain B510 were designed and evaluated by colony and endpoint PCR.  The CRISPRloci-PCR approach was standardized for Azospirillum sp.  The CRISPRloci-PCR approach was validated in assays with substrate and wheat seedlings.  The CRISPRloci-PCR approach was found to be a useful molecular tool for specific tracking of Azospirillum at the strain level. 
PCR
CRISPR loci-PCR as Tool for Tracking Azospirillum sp.  Primer sets for Azospirillum sp.  The CRISPRloci-PCR approach was standardized for Azospirillum sp.  Azospirillum sp. 
Azospirillum sp.
Strain B510.Azospirillum-based plant and soil inoculants are widely used in agriculture.  strain B510 were designed and evaluated by colony and endpoint PCR.  strain B510, and its specificity was observed by testing against 9 different Azospirillum strains, and 38 strains of diverse bacterial genera isolated from wheat plants.  strain B510 was detected after of two weeks of inoculation in both sterile and nonsterile substrates as well as rhizosphere grown in sterile substrate. 
strain B510
In this context, clustered regularly interspaced short palindromic repeats (CRISPR) loci structure is unique in the bacterial genome, including some Azospirillum species. 
clustered regularly interspaced short palindromic repeats
In this context, clustered regularly interspaced short palindromic repeats (CRISPR) loci structure is unique in the bacterial genome, including some Azospirillum species. 
bacterial genome
In this context, clustered regularly interspaced short palindromic repeats (CRISPR) loci structure is unique in the bacterial genome, including some Azospirillum species. 
Azospirillum species
strain B510 were designed and evaluated by colony and endpoint PCR. 
endpoint PCR
Primer sets for Azospirillum sp. 
primer sets
The inoculated Azospirillum strains are commonly tracked by both culture-dependent and culture-independent methods, which are time-consuming or expensive.  Here, we investigated the use of CRISPR loci to track specific Azospirillum strains in soils systems by PCR.  strain B510, and its specificity was observed by testing against 9 different Azospirillum strains, and 38 strains of diverse bacterial genera isolated from wheat plants. 
Azospirillum strains
strain B510, and its specificity was observed by testing against 9 different Azospirillum strains, and 38 strains of diverse bacterial genera isolated from wheat plants. 
bacterial genera
strain B510, and its specificity was observed by testing against 9 different Azospirillum strains, and 38 strains of diverse bacterial genera isolated from wheat plants. 
wheat plants
The CRISPRloci-PCR approach was validated in assays with substrate and wheat seedlings.  strain B510 was detected after of two weeks of inoculation in both sterile and nonsterile substrates as well as rhizosphere grown in sterile substrate. 
substrate
The CRISPRloci-PCR approach was validated in assays with substrate and wheat seedlings. 
seedlings
strain B510 was detected after of two weeks of inoculation in both sterile and nonsterile substrates as well as rhizosphere grown in sterile substrate. 
rhizosphere
strain B510 was detected after of two weeks of inoculation in both sterile and nonsterile substrates as well as rhizosphere grown in sterile substrate. 
sterile substrate
strain B510 was detected after of two weeks of inoculation in both sterile and nonsterile substrates as well as rhizosphere grown in sterile substrate. 
nonsterile substrates