EWS-FLI1 induction and Stag2 knockdown were achieved in vitro by adenovirus-Cre and shRNA-bearing pGIPZ lentiviral infection, respectively. 
adenovirus-Cre
EWS-FLI1 induction and Stag2 knockdown were achieved in vitro by adenovirus-Cre and shRNA-bearing pGIPZ lentiviral infection, respectively. 
shRNA-bearing pGIPZ lentiviral infection
Cells were injected with Matrigel intramuscularly into C57/Bl6 mice to test for tumor formation.



Matrigel