CRISPR-based targeted haplotype-resolved assembly of a megabase region.Constructing high-quality haplotype-resolved genome assemblies has substantially improved the ability to detect and characterize genetic variants.  Here, using the 4.5 megabase, notoriously difficult-to-assemble major histocompatibility complex (MHC) region as an example, we demonstrated an approach to construct haplotype-resolved assembly of the targeted genomic region with the CRISPR-based enrichment. 
CRISPR
Here, using the 4.5 megabase, notoriously difficult-to-assemble major histocompatibility complex (MHC) region as an example, we demonstrated an approach to construct haplotype-resolved assembly of the targeted genomic region with the CRISPR-based enrichment. 
CRISPR-based enrichment