Mapping O-glycosylation Sites Using OpeRATOR and LC-MS.O-glycosylation is a difficult posttranslational modification to analyze.  OpeRATOR is an O-glycan specific protease that cleaves the protein backbone N-terminally of glycosylated serine and threonine residues.  In this chapter we demonstrate a simple workflow for in-depth analysis of O-glycosylation sites on heavily glycosylated proteins using OpeRATOR digestion and HILIC-MS/MS analysis.
OpeRATOR