Here we apply single-cell sequencing to a mouse model of kidney fibrosis. 
single-cell sequencing
Here we apply single-cell sequencing to a mouse model of kidney fibrosis. 
mouse model
Receptor-ligand interaction analysis and experimental validation indicate that CXCL1 secreted by profibrotic tubules recruits CXCR2+ basophils. 
CXCL1
Receptor-ligand interaction analysis and experimental validation indicate that CXCL1 secreted by profibrotic tubules recruits CXCR2+ basophils. 
CXCR2+ basophils
In mice, these basophils are an important source of interleukin-6 and recruitment of the TH17 subset of helper T cells. 
interleukin-6
In mice, these basophils are an important source of interleukin-6 and recruitment of the TH17 subset of helper T cells. 
TH17 subset of helper T cells
Single-cell analysis identifies the interaction of altered renal tubules with basophils orchestrating kidney fibrosis.Inflammation is an important component of fibrosis but immune processes that orchestrate kidney fibrosis are not well understood.  Receptor-ligand interaction analysis and experimental validation indicate that CXCL1 secreted by profibrotic tubules recruits CXCR2+ basophils.  In mice, these basophils are an important source of interleukin-6 and recruitment of the TH17 subset of helper T cells.  Genetic deletion or antibody-based depletion of basophils results in reduced renal fibrosis.  Human kidney single-cell, bulk gene expression and immunostaining validate a function for basophils in patients with kidney fibrosis.  Collectively, these studies identify basophils as contributors to the development of renal fibrosis and suggest that targeting these cells might be a useful clinical strategy to manage chronic kidney disease.
basophils