SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology.Phage display is an established method for the in vitro selection of recombinant antibodies and other proteins or peptides from gene libraries.  Here we describe SpyDisplay, a phage display method in which the display is achieved via SpyTag/SpyCatcher protein ligation instead of genetically fusing the displayed protein to a phage coat protein.  Furthermore, SpyDisplay streamlines additional applications that have traditionally been challenging for phage display: we show that it can be applied to N-terminal display of the protein of interest and it enables display of cytoplasmically folding proteins exported to periplasm via the TAT pathway.
SpyDisplay
SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology.Phage display is an established method for the in vitro selection of recombinant antibodies and other proteins or peptides from gene libraries. 
SpyTag/SpyCatcher technology
SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology.Phage display is an established method for the in vitro selection of recombinant antibodies and other proteins or peptides from gene libraries. 
phage display selection system