Comparison of skin biopsy sample processing and storage methods on high dimensional immune gene expression using the Nanostring nCounter system.BACKGROUND

Nanostring nCounter system
RNA yield and integrity was assessed following extraction via NanoDrop, QuantiFluor with RNA specific dye and a Bioanalyser (LabChip24, PerkinElmer). 
NanoDrop
RNA yield and integrity was assessed following extraction via NanoDrop, QuantiFluor with RNA specific dye and a Bioanalyser (LabChip24, PerkinElmer). 
QuantiFluor with RNA specific dye
RNA yield and integrity was assessed following extraction via NanoDrop, QuantiFluor with RNA specific dye and a Bioanalyser (LabChip24, PerkinElmer). 
Bioanalyser (LabChip24, PerkinElmer)
Immune gene expression was analysed using the NanoString Pancancer Immune Profiling Panel containing 730 genes.



NanoString Pancancer Immune Profiling Panel