PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions.Polycomb group (PcG) ring finger protein 6 (PCGF6), though known as a member of the transcription-repressing complexes, PcG, also has activation function in regulating pluripotency gene expression.  However, the mechanism underlying the activation function of PCGF6 is poorly understood.  Here, we found that PCGF6 co-localizes to gene activation regions along with pluripotency factors such as OCT4.  In addition, PCGF6 was recruited to a subset of the super-enhancer (SE) regions upstream of cell cycle-associated genes by OCT4, and increased their expression.  By combining with promoter capture Hi-C data, we found that PCGF6 activates cell cycle genes by regulating SE-promoter interactions via 3D chromatin. 
PCGF6
PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions.Polycomb group (PcG) ring finger protein 6 (PCGF6), though known as a member of the transcription-repressing complexes, PcG, also has activation function in regulating pluripotency gene expression.  However, the mechanism underlying the activation function of PCGF6 is poorly understood.  Here, we found that PCGF6 co-localizes to gene activation regions along with pluripotency factors such as OCT4.  In addition, PCGF6 was recruited to a subset of the super-enhancer (SE) regions upstream of cell cycle-associated genes by OCT4, and increased their expression.  By combining with promoter capture Hi-C data, we found that PCGF6 activates cell cycle genes by regulating SE-promoter interactions via 3D chromatin.  Our findings highlight a novel mechanism of PcG protein in regulating pluripotency, and provide a research basis for the therapeutic application of pluripotent stem cells.
PcG
Here, we found that PCGF6 co-localizes to gene activation regions along with pluripotency factors such as OCT4.  In addition, PCGF6 was recruited to a subset of the super-enhancer (SE) regions upstream of cell cycle-associated genes by OCT4, and increased their expression. 
OCT4
PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions.Polycomb group (PcG) ring finger protein 6 (PCGF6), though known as a member of the transcription-repressing complexes, PcG, also has activation function in regulating pluripotency gene expression.  In addition, PCGF6 was recruited to a subset of the super-enhancer (SE) regions upstream of cell cycle-associated genes by OCT4, and increased their expression. 
super-enhancer
PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions.Polycomb group (PcG) ring finger protein 6 (PCGF6), though known as a member of the transcription-repressing complexes, PcG, also has activation function in regulating pluripotency gene expression. 
chromatin interactions