AP profiling resolves co-translational folding pathway and chaperone interactions in vivo.Natural proteins have evolved to fold robustly along specific pathways.  We developed a high-throughput method to quantify co-translational folding in live cells that we term Arrest Peptide profiling (AP profiling).  We employed AP profiling to delineate co-translational folding for a set of GTPase domains with very similar structures, defining how topology shapes folding pathways. 
AP profiling
AP profiling resolves co-translational folding pathway and chaperone interactions in vivo.Natural proteins have evolved to fold robustly along specific pathways.  We developed a high-throughput method to quantify co-translational folding in live cells that we term Arrest Peptide profiling (AP profiling).  We employed AP profiling to delineate co-translational folding for a set of GTPase domains with very similar structures, defining how topology shapes folding pathways. 
AP profiling
AP profiling resolves co-translational folding pathway and chaperone interactions in vivo.Natural proteins have evolved to fold robustly along specific pathways.  We developed a high-throughput method to quantify co-translational folding in live cells that we term Arrest Peptide profiling (AP profiling).  We employed AP profiling to delineate co-translational folding for a set of GTPase domains with very similar structures, defining how topology shapes folding pathways. 
AP profiling