The expression level of miR-7-5p was confirmed by qRT-PCR in lung cancer cell lines.  Western blot assays and qRT-PCR were performed to demonstrate miR-7-5p's effect. 
qRT-PCR
Western blot assays and qRT-PCR were performed to demonstrate miR-7-5p's effect. 
Western blot assays
The binding sites of miR-7-5p in the OGT 3' UTR were verified by luciferase reporter assay. 
Luciferase reporter assay
To investigate the role of miR-7-5p in the cancer metabolism of non-small cell lung cancer (NSCLC) cells, mimic of miR-7-5p was transfected into NSCLC cells, and the effect of miR-7-5p on cancer metabolism was analyzed by LDH assays, glucose uptake, and mitochondrial ATP synthase inhibitor assay. 
LDH assays
To investigate the role of miR-7-5p in the cancer metabolism of non-small cell lung cancer (NSCLC) cells, mimic of miR-7-5p was transfected into NSCLC cells, and the effect of miR-7-5p on cancer metabolism was analyzed by LDH assays, glucose uptake, and mitochondrial ATP synthase inhibitor assay. 
Glucose uptake
To investigate the role of miR-7-5p in the cancer metabolism of non-small cell lung cancer (NSCLC) cells, mimic of miR-7-5p was transfected into NSCLC cells, and the effect of miR-7-5p on cancer metabolism was analyzed by LDH assays, glucose uptake, and mitochondrial ATP synthase inhibitor assay. 
Mitochondrial ATP synthase inhibitor assay
The role of miR-7-5p in lung cancer growth was measured by MTS assays. 
MTS assays