Patient data and serum samples were collected and liver disease related indicators were measured to detect ENO1 levels with enzyme-linked immunosorbent assay (ELISA). 
ELISA
Mann-Whitney U nonparametric test was used to analyze the differences between the data. 
Mann-Whitney U nonparametric test
A Spearman's correlation analysis was used for bivariate correlation analysis. 
Spearman's correlation analysis
The sensitivity and specificity of ENO1 and alpha-fetoprotein in the diagnosis of liver cancer were analyzed by ROC curve. 
ROC curve