Development and validation of multiplex PCR assay for differentiating tunas and billfishes.Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two.  Tunas and billfishes primers were designed on the cytochrome oxidase subunit I. Multiplex PCR bands obtained were 579 bp, 291 bp and 114 bp for tunas, billfishes and internal control.  The newly developed multiplex PCR assay is a time and cost effective technique for determining the authenticity of tunas and billfishes.
multiplex PCR
Development and validation of multiplex PCR assay for differentiating tunas and billfishes.Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two.  Tunas and billfishes primers were designed on the cytochrome oxidase subunit I. Multiplex PCR bands obtained were 579 bp, 291 bp and 114 bp for tunas, billfishes and internal control.  The newly developed multiplex PCR assay is a time and cost effective technique for determining the authenticity of tunas and billfishes.
PCR
Development and validation of multiplex PCR assay for differentiating tunas and billfishes.Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two.  Tunas and billfishes primers were designed on the cytochrome oxidase subunit I. Multiplex PCR bands obtained were 579 bp, 291 bp and 114 bp for tunas, billfishes and internal control.  The newly developed multiplex PCR assay is a time and cost effective technique for determining the authenticity of tunas and billfishes.
PCR
Development and validation of multiplex PCR assay for differentiating tunas and billfishes.Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two.  Tunas and billfishes primers were designed on the cytochrome oxidase subunit I. Multiplex PCR bands obtained were 579 bp, 291 bp and 114 bp for tunas, billfishes and internal control.  The newly developed multiplex PCR assay is a time and cost effective technique for determining the authenticity of tunas and billfishes.
PCR
Development and validation of multiplex PCR assay for differentiating tunas and billfishes.Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two.  Tunas and billfishes primers were designed on the cytochrome oxidase subunit I. Multiplex PCR bands obtained were 579 bp, 291 bp and 114 bp for tunas, billfishes and internal control.  The newly developed multiplex PCR assay is a time and cost effective technique for determining the authenticity of tunas and billfishes.
PCR
Development and validation of multiplex PCR assay for differentiating tunas and billfishes.Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two.  Tunas and billfishes primers were designed on the cytochrome oxidase subunit I. Multiplex PCR bands obtained were 579 bp, 291 bp and 114 bp for tunas, billfishes and internal control.  The newly developed multiplex PCR assay is a time and cost effective technique for determining the authenticity of tunas and billfishes.
PCR