Transcription factor chromatin profiling genome-wide using uliCUT&RUN in single cells and individual blastocysts.Determining chromatin-associated protein localization across the genome has provided insight into the functions of DNA-binding proteins and their connections to disease.  We recently described a protocol to profile chromatin-associated protein localization in as low as one cell: ultra-low-input cleavage under targets and release using nuclease (uliCUT&RUN).  Optimized from chromatin immunocleavage and CUT&RUN, uliCUT&RUN is a tethered enzyme-based protocol that utilizes a combination of recombinant protein, antibody recognition and stringent purification to selectively target proteins of interest and isolate the associated DNA.  Performed in native conditions, uliCUT&RUN profiles protein localization to chromatin with low input and high precision.  Compared with other profiling technologies, uliCUT&RUN can determine nonhistone protein chromatin occupancies in low cell numbers, permitting the investigation into the molecular functions of a range of DNA-binding proteins within rare samples.  From sample preparation to sequencing library submission, the uliCUT&RUN protocol takes <2 d to perform, with the accompanying data analysis timeline dependent on experience level.
uliCUT&RUN
Transcription factor chromatin profiling genome-wide using uliCUT&RUN in single cells and individual blastocysts.Determining chromatin-associated protein localization across the genome has provided insight into the functions of DNA-binding proteins and their connections to disease.  We recently described a protocol to profile chromatin-associated protein localization in as low as one cell: ultra-low-input cleavage under targets and release using nuclease (uliCUT&RUN).  Optimized from chromatin immunocleavage and CUT&RUN, uliCUT&RUN is a tethered enzyme-based protocol that utilizes a combination of recombinant protein, antibody recognition and stringent purification to selectively target proteins of interest and isolate the associated DNA.  Performed in native conditions, uliCUT&RUN profiles protein localization to chromatin with low input and high precision.  Compared with other profiling technologies, uliCUT&RUN can determine nonhistone protein chromatin occupancies in low cell numbers, permitting the investigation into the molecular functions of a range of DNA-binding proteins within rare samples.  From sample preparation to sequencing library submission, the uliCUT&RUN protocol takes <2 d to perform, with the accompanying data analysis timeline dependent on experience level.
uliCUT&RUN
Transcription factor chromatin profiling genome-wide using uliCUT&RUN in single cells and individual blastocysts.Determining chromatin-associated protein localization across the genome has provided insight into the functions of DNA-binding proteins and their connections to disease.  We recently described a protocol to profile chromatin-associated protein localization in as low as one cell: ultra-low-input cleavage under targets and release using nuclease (uliCUT&RUN).  Optimized from chromatin immunocleavage and CUT&RUN, uliCUT&RUN is a tethered enzyme-based protocol that utilizes a combination of recombinant protein, antibody recognition and stringent purification to selectively target proteins of interest and isolate the associated DNA.  Performed in native conditions, uliCUT&RUN profiles protein localization to chromatin with low input and high precision.  Compared with other profiling technologies, uliCUT&RUN can determine nonhistone protein chromatin occupancies in low cell numbers, permitting the investigation into the molecular functions of a range of DNA-binding proteins within rare samples.  From sample preparation to sequencing library submission, the uliCUT&RUN protocol takes <2 d to perform, with the accompanying data analysis timeline dependent on experience level.
uliCUT&RUN