Medium-throughput in vitro detection of DNA cleavage by CRISPR-Cas12a.Quantifying DNA cleavage by CRISPR-Cas nucleases is usually done by separating the cleaved products from the non-cleaved target by agarose gel electrophoresis. 
CRISPR-Cas12a
Medium-throughput in vitro detection of DNA cleavage by CRISPR-Cas12a.Quantifying DNA cleavage by CRISPR-Cas nucleases is usually done by separating the cleaved products from the non-cleaved target by agarose gel electrophoresis. 
agarose gel electrophoresis
Cleavage of the target will separate the dye from the biotin, and cause the dye to stay in solution when streptavidin beads are introduced. 
streptavidin beads