Sanger Gap Sequencing for Genetic Alphabet Expansion of DNA.Genetic alphabet expansion technology, creating new replicable and functional DNA molecules with unnatural base pairs (UBPs), is the novel promising research area of xenobiology.  Here, we present an improved Sanger sequencing method (Sanger gap sequencing) for DNAs containing our UBP, Ds-Px, which appears as gaps in the sequencing peak patterns. 
Sanger gap sequencing
Sanger Gap Sequencing for Genetic Alphabet Expansion of DNA.Genetic alphabet expansion technology, creating new replicable and functional DNA molecules with unnatural base pairs (UBPs), is the novel promising research area of xenobiology.  Here, we present an improved Sanger sequencing method (Sanger gap sequencing) for DNAs containing our UBP, Ds-Px, which appears as gaps in the sequencing peak patterns. 
Sanger gap sequencing
However, all of the conventional sequencing methods, such as Sanger methods, are for four-letter DNA molecules. 
Sanger methods
Sanger Gap Sequencing for Genetic Alphabet Expansion of DNA.Genetic alphabet expansion technology, creating new replicable and functional DNA molecules with unnatural base pairs (UBPs), is the novel promising research area of xenobiology.  Here, we present an improved Sanger sequencing method (Sanger gap sequencing) for DNAs containing our UBP, Ds-Px, which appears as gaps in the sequencing peak patterns. 
Sanger gap sequencing