Flash Nanoprecipitation Fabrication of PEI@Amorphous Calcium Carbonate Hybrid Nanoparticles for siRNA Delivery.RNA interference (RNAi) is a promising approach for disease treatments.  In this study, we prepared pH responsive amorphous calcium carbonate NPs (ACC NPs) using flash nanoprecipitation (FNP) and constructed hybrid NPs by coating ACC NPs with polyethyleneimine (PEI) for efficient siRNA delivery. 
Flash Nanoprecipitation
In this study, we prepared pH responsive amorphous calcium carbonate NPs (ACC NPs) using flash nanoprecipitation (FNP) and constructed hybrid NPs by coating ACC NPs with polyethyleneimine (PEI) for efficient siRNA delivery. 
FNP
Flash Nanoprecipitation Fabrication of PEI@Amorphous Calcium Carbonate Hybrid Nanoparticles for siRNA Delivery.RNA interference (RNAi) is a promising approach for disease treatments.  In this study, we prepared pH responsive amorphous calcium carbonate NPs (ACC NPs) using flash nanoprecipitation (FNP) and constructed hybrid NPs by coating ACC NPs with polyethyleneimine (PEI) for efficient siRNA delivery.  PEI/ACC NPs show robust pH responsiveness and stability as well as effective siRNA loading and protection.  Furthermore, siRNA-loaded PEI/ACC NPs demonstrate enhanced cellular uptake and efficient endosomal escape, mediating improved siRNA delivery compared to pure PEI.  These findings suggest that PEI/ACC NPs may have great potential in siRNA delivery for RNAi-based therapy. 
PEI
In this study, we prepared pH responsive amorphous calcium carbonate NPs (ACC NPs) using flash nanoprecipitation (FNP) and constructed hybrid NPs by coating ACC NPs with polyethyleneimine (PEI) for efficient siRNA delivery.  PEI/ACC NPs show robust pH responsiveness and stability as well as effective siRNA loading and protection.  Furthermore, siRNA-loaded PEI/ACC NPs demonstrate enhanced cellular uptake and efficient endosomal escape, mediating improved siRNA delivery compared to pure PEI.  These findings suggest that PEI/ACC NPs may have great potential in siRNA delivery for RNAi-based therapy. 
ACC NPs
PEI/ACC NPs show robust pH responsiveness and stability as well as effective siRNA loading and protection.  Furthermore, siRNA-loaded PEI/ACC NPs demonstrate enhanced cellular uptake and efficient endosomal escape, mediating improved siRNA delivery compared to pure PEI.  These findings suggest that PEI/ACC NPs may have great potential in siRNA delivery for RNAi-based therapy. 
PEI/ACC NPs