A competition smFRET assay to study ligand-induced conformational changes of the dengue virus protease.Ligand binding to proteins often is accompanied by conformational transitions.  Here, we describe a competition assay based on single molecule Förster resonance energy transfer (smFRET) to investigate the ligand-induced conformational changes of the dengue virus (DENV) NS2B-NS3 protease, which can adopt at least two different conformations. 
smFRET
Here, we describe a competition assay based on single molecule Förster resonance energy transfer (smFRET) to investigate the ligand-induced conformational changes of the dengue virus (DENV) NS2B-NS3 protease, which can adopt at least two different conformations. 
single molecule Förster resonance energy transfer