TRPV1 expression was examined in liver biopsies from patients with liver fibrosis using quantitative real-time PCR (qPCR) and immunostaining. 
qPCR
Its contribution to liver fibrosis was examined in Trpv1-/- mice, upon lentiviral delivery of the TRPV1 gene, and in human and mouse primary HSC, using patch clamp, intracellular Ca2+ mobilization determination, FACS analyses and gain/loss of function experiments. 
lentiviral delivery
Its contribution to liver fibrosis was examined in Trpv1-/- mice, upon lentiviral delivery of the TRPV1 gene, and in human and mouse primary HSC, using patch clamp, intracellular Ca2+ mobilization determination, FACS analyses and gain/loss of function experiments. 
patch clamp
Its contribution to liver fibrosis was examined in Trpv1-/- mice, upon lentiviral delivery of the TRPV1 gene, and in human and mouse primary HSC, using patch clamp, intracellular Ca2+ mobilization determination, FACS analyses and gain/loss of function experiments. 
FACS analyses
Binding of sterile alpha and Toll/interleukin-1 receptor motif-containing protein 1 (SARM1) to TRPV1 was determined using mass spectrometry, co-immunoprecipitation, surface plasmon resonance (SPR), bioluminescence resonance energy transfer (BRET), and NanoBiT.



mass spectrometry
Binding of sterile alpha and Toll/interleukin-1 receptor motif-containing protein 1 (SARM1) to TRPV1 was determined using mass spectrometry, co-immunoprecipitation, surface plasmon resonance (SPR), bioluminescence resonance energy transfer (BRET), and NanoBiT.



co-immunoprecipitation
Binding of sterile alpha and Toll/interleukin-1 receptor motif-containing protein 1 (SARM1) to TRPV1 was determined using mass spectrometry, co-immunoprecipitation, surface plasmon resonance (SPR), bioluminescence resonance energy transfer (BRET), and NanoBiT.



surface plasmon resonance (SPR)
Binding of sterile alpha and Toll/interleukin-1 receptor motif-containing protein 1 (SARM1) to TRPV1 was determined using mass spectrometry, co-immunoprecipitation, surface plasmon resonance (SPR), bioluminescence resonance energy transfer (BRET), and NanoBiT.



bioluminescence resonance energy transfer (BRET)
Binding of sterile alpha and Toll/interleukin-1 receptor motif-containing protein 1 (SARM1) to TRPV1 was determined using mass spectrometry, co-immunoprecipitation, surface plasmon resonance (SPR), bioluminescence resonance energy transfer (BRET), and NanoBiT.



NanoBiT