Using IRM, together with the image analysis software described in this paper, the field of view and the frame rate is limited only by the camera; with a sCMOS camera and wide-field illumination microtubule length can be measured with precision up to 20 nm with a bandwidth of 10 Hz.
image analysis software
In the case of microtubules, we have found that images of similar quality to TIRF can be obtained using interference reflection microscopy (IRM).  This suggests that IRM might be a general technique for visualizing the dynamics of large biomolecules and oligomers in vitro.  In this paper, we show how a fluorescence microscope can be modified simply to obtain IRM images.  IRM is easier and considerably cheaper to implement than other contrast techniques such as differential interference contrast microcopy or interferometric scattering microscopy.  Using IRM, together with the image analysis software described in this paper, the field of view and the frame rate is limited only by the camera; with a sCMOS camera and wide-field illumination microtubule length can be measured with precision up to 20 nm with a bandwidth of 10 Hz.
IRM
It is also less susceptible to surface defects and solution impurities than darkfield microscopy. 
darkfield microscopy
IRM is easier and considerably cheaper to implement than other contrast techniques such as differential interference contrast microcopy or interferometric scattering microscopy. 
interferometric scattering microscopy