Extracellular thiamine concentration influences thermogenic competency of differentiating neck area-derived human adipocytes.Introduction
 Therefore, finding novel agents that can stimulate the differentiation and recruitment of brown or beige thermogenic adipocytes are important subjects for investigation.  The current study investigated how the availability of extracellular thiamine (vitamin B1), an essential cofactor of mitochondrial enzyme complexes that catalyze key steps in the catabolism of nutrients, affects the expression of thermogenic marker genes and proteins and subsequent functional parameters during ex vivo adipocyte differentiation.


 mRNA and protein expression of thermogenic genes were analyzed by RT-qPCR and western blot, respectively.  Providing abundant amounts of thiamine further increased the thermogenic competency of the adipocytes.


 Our study raises the possibility of a novel strategy with long-term thiamine supplementation, which can enhance the thermogenic competency of differentiating neck area-derived adipocytes for preventing or combating obesity.
THERM