Novel ceRNA network construction associated with programmed cell death in acute rejection of heart allograft in mice.Background
 The biological functions of ceRNA networks was analyzed by GO and KEGG enrichment.  Differentially expressed ceRNA involved in programmed cell death were further verified with qRT-PCR testing.


 Based on the ceRNA theory, lncRNA-miRNA-mRNA regulatory networks were constructed in allograft acute rejection response.  Four lncRNA-mediated ceRNA regulatory pathways, Pvt1/miR-30c-5p/Pdgfc, 1700071M16Rik/miR-145a-3p/Pdgfc, 1700071M16Rik/miR-145a-3p/Tox, 1700071M16Rik/miR-145a-3p/Themis2, were finally validated. 
ceRNA
This study aimed to explore the novel lncRNA-miRNA-mRNA networks that contributed to AR in a mouse heart transplantation model.



miRNA-mRNA networks
The whole-transcriptome sequencing was performed to obtain differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs) and lncRNAs (DElncRNAs) in mouse heart allograft. 
whole-transcriptome sequencing
Differentially expressed ceRNA involved in programmed cell death were further verified with qRT-PCR testing.



qRT-PCR testing