A new MATLAB customized algorithm to measure the horizontal width of the four main zones of ortho-k fluorescein patterns (central bearing, tear reservoir, mid-peripheral bearing and edge lift) was designed. 
MATLAB
The agreement between two independent observers and the ImageJ measurements was determined.


 The new clinical tool provided similar measurements to ImageJ software for the central bearing (4.20 ± 0.74 and 4.27 ± 0.69 mm; P = 0.21), tear reservoir (1.69 ± 0.41 and 1.69 ± 0.45 mm; P = 0.69), mid-peripheral bearing (1.17 ± 0.11 and 1.13 ± 0.10 mm; P < 0.01) and edge lift (0.48 ± 0.06 and 0.48 ± 0.06 mm; P = 0.81) zones. 
ImageJ