Methylation-specific PCR (MSP) revealed that GAS5 negatively regulated the methylation of miR-124. 
MSP
LncRNA GAS5 upregulates miR-214 through methylation to participate in cell apoptosis of sepsis.It has been reported that lncRNA GAS5 can inhibit LPS-induced inflammation, indicating its involvement in sepsis.  Cell apoptosis showed that overexpression of GAS5 and miR-214 suppressed the apoptosis of cardiomyocytes induced by LPS. 
LPS