Using limma package, the differentially expressed genes (DEGs) between samples from AD and healthy control were selected. 
limma
Expression profile data (GSE75890) were obtained from the database of Gene Expression Omnibus. 
Gene Expression Omnibus
And quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the expressions patterns of key genes.


 Moreover, the upregulation of these genes, including CCNB1, CCNB2, CCNA2, CXCL10, and CXCL9 were confirmed by qRT-PCR.



qRT-PCR