Serum levels of circ_0000175 and miR-31-5p were determined by RT-qPCR, and the correlation between them was evaluated by Spearman correlation analysis. 
RT-qPCR
Serum levels of circ_0000175 and miR-31-5p were determined by RT-qPCR, and the correlation between them was evaluated by Spearman correlation analysis. 
Spearman correlation analysis
The subcellular localization of circ_0000175 was detected by FISH assay. 
FISH assay
Pyroptosis and inflammatory cytokines interleukin (IL)-1β, IL-18 and IL-6 were measured by flow cytometry and ELISA, respectively. 
flow cytometry
Pyroptosis and inflammatory cytokines interleukin (IL)-1β, IL-18 and IL-6 were measured by flow cytometry and ELISA, respectively. 
ELISA
RNA pull-down and luciferase reporter assays verified the interaction between circ_0000175 and miR-31-5p. 
RNA pull-down
RNA pull-down and luciferase reporter assays verified the interaction between circ_0000175 and miR-31-5p. 
luciferase reporter assays
Western blot was used to detect the differential expression of pyroptosis-related factors (GSDME-N, GSDMD-N, cleaved caspase-1 and cleaved caspase-3). 
Western blot