In this study, the comprehensive activity of representative ER ligands was evaluated by using BRET-based ERα and ERβ dimerization and ER transactivation assays to differentiate the specific binding and function of ERα and ERβ from 12 representative natural and synthetic estrogenic substances.  Overall, this study demonstrated consistency between BRET dimerization and transactivation responses, supporting potential supplementary application of mechanism-based BRET assays as high-throughput screening methods for evaluation of potential endocrine-disrupting activity of environmental agents. 
BRET