BG-flow, a new flow cytometry tool for G-quadruplex quantification in fixed cells.BACKGROUND
 Here, we assembled a novel protocol that uses the G4-specific antibody BG4 to quantify G4 structures by flow cytometry (BG-flow).


 We provide mechanistic proof that BG-flow is working in different kinds of cells ranging from mouse to humans.  We propose that BG-flow can be combined with additional antibodies for cell surface markers to determine G4 structures in subpopulations of cells, which will be beneficial to address the relevance and consequences of G4 structures in mixed cell populations. 
BG-flow
BG-flow, a new flow cytometry tool for G-quadruplex quantification in fixed cells.BACKGROUND

flow cytometry tool
BG-flow, a new flow cytometry tool for G-quadruplex quantification in fixed cells.BACKGROUND

G-quadruplex quantification
BG-flow, a new flow cytometry tool for G-quadruplex quantification in fixed cells.BACKGROUND

fixed cells