Generation of CoilR Probe Peptides for VIPER-labeling of Cellular Proteins.Versatile Interacting Peptide (VIP) tags are a new class of genetically-encoded tag designed for imaging cellular proteins by fluorescence and electron microscopy.  In 2018, we reported the VIPER tag ( Doh et al., 2018 ), which contains two elements: a genetically-encoded peptide tag (i.e., CoilE) and a probe peptide (i.e., CoilR).  This protocol is complemented by two other Bio-Protocols outlining the use of VIPER ( Doh et al., 2019a and 2019b).
VIPER
Generation of CoilR Probe Peptides for VIPER-labeling of Cellular Proteins.Versatile Interacting Peptide (VIP) tags are a new class of genetically-encoded tag designed for imaging cellular proteins by fluorescence and electron microscopy.  In 2018, we reported the VIPER tag ( Doh et al., 2018 ), which contains two elements: a genetically-encoded peptide tag (i.e., CoilE) and a probe peptide (i.e., CoilR).  In this Bio-Protocol, we describe our methods for expressing and purifying recombinant CoilR. 
CoilR
Generation of CoilR Probe Peptides for VIPER-labeling of Cellular Proteins.Versatile Interacting Peptide (VIP) tags are a new class of genetically-encoded tag designed for imaging cellular proteins by fluorescence and electron microscopy.  In 2018, we reported the VIPER tag ( Doh et al., 2018 ), which contains two elements: a genetically-encoded peptide tag (i.e., CoilE) and a probe peptide (i.e., CoilR).  This protocol is complemented by two other Bio-Protocols outlining the use of VIPER ( Doh et al., 2019a and 2019b).
VIPER