The type IIS restriction enzyme MmeI can cut across a double-strand break.BACKGROUND
 We studied the ability of the Type-IIS restriction enzyme MmeI, which recognizes an asymmetric sequence TCCRAC and cuts 20 bp downstream, to cut across a double-strand break (DSB).


 We used synthetic double-stranded oligos with MmeI recognition sites close to 5' end and different overhang lengths to measure digestion after different periods of time and at different temperatures.  We found that the MmeI binding and cutting sites can be situated on opposite sides of a DSB if the edges of the DNA molecules are held together by transient base-pairing interactions between compatible overhangs.


 We found that MmeI can cut across a DSB, and the efficiency of the cutting depends on both overhang length and temperature.
MmeI
The type IIS restriction enzyme MmeI can cut across a double-strand break.BACKGROUND
 We studied the ability of the Type-IIS restriction enzyme MmeI, which recognizes an asymmetric sequence TCCRAC and cuts 20 bp downstream, to cut across a double-strand break (DSB).


 We used synthetic double-stranded oligos with MmeI recognition sites close to 5' end and different overhang lengths to measure digestion after different periods of time and at different temperatures.  We found that the MmeI binding and cutting sites can be situated on opposite sides of a DSB if the edges of the DNA molecules are held together by transient base-pairing interactions between compatible overhangs.


 We found that MmeI can cut across a DSB, and the efficiency of the cutting depends on both overhang length and temperature.
MmeI
The type IIS restriction enzyme MmeI can cut across a double-strand break.BACKGROUND
 We studied the ability of the Type-IIS restriction enzyme MmeI, which recognizes an asymmetric sequence TCCRAC and cuts 20 bp downstream, to cut across a double-strand break (DSB).


 We used synthetic double-stranded oligos with MmeI recognition sites close to 5' end and different overhang lengths to measure digestion after different periods of time and at different temperatures.  We found that the MmeI binding and cutting sites can be situated on opposite sides of a DSB if the edges of the DNA molecules are held together by transient base-pairing interactions between compatible overhangs.


 We found that MmeI can cut across a DSB, and the efficiency of the cutting depends on both overhang length and temperature.
MmeI