Single-particle reconstruction statistics: a diagnostic tool in solving biomolecular structures by cryo-EM.In single-particle analysis (SPA), the aim is to obtain a 3D reconstruction of a biological molecule from 2D electron micrographs to the highest level of detail or resolution as possible. 
cryo-EM
Single-particle reconstruction statistics: a diagnostic tool in solving biomolecular structures by cryo-EM.In single-particle analysis (SPA), the aim is to obtain a 3D reconstruction of a biological molecule from 2D electron micrographs to the highest level of detail or resolution as possible. 
single-particle analysis (SPA)
Single-particle reconstruction statistics: a diagnostic tool in solving biomolecular structures by cryo-EM.In single-particle analysis (SPA), the aim is to obtain a 3D reconstruction of a biological molecule from 2D electron micrographs to the highest level of detail or resolution as possible.  This study revisits the theory of 3D reconstruction and demonstrates how the associated statistics can provide a diagnostic tool to improve SPA. 
3D reconstruction