Development of a simple PCR-RFLP technique for detection and differentiation of E. histolytica, E. dispar and E. moshkovskii.Epidemiological studies on amoebic infections are complicated by morphological overlap between the pathogenic E. histolytica, the commensal E. dispar and the amphizoic E. moshkovskii, necessitating molecular identification.  The present study developed a simple and economical 18S PCR-RFLP method for the simultaneous detection and differentiation of the three species.  Analysis indicated that the PCR-RFLP could successfully differentiate between the three species and was confirmed by sequence analysis. 
PCR-RFLP
Development of a simple PCR-RFLP technique for detection and differentiation of E. histolytica, E. dispar and E. moshkovskii.Epidemiological studies on amoebic infections are complicated by morphological overlap between the pathogenic E. histolytica, the commensal E. dispar and the amphizoic E. moshkovskii, necessitating molecular identification.  The present study developed a simple and economical 18S PCR-RFLP method for the simultaneous detection and differentiation of the three species.  Analysis indicated that the PCR-RFLP could successfully differentiate between the three species and was confirmed by sequence analysis. 
PCR-RFLP
Development of a simple PCR-RFLP technique for detection and differentiation of E. histolytica, E. dispar and E. moshkovskii.Epidemiological studies on amoebic infections are complicated by morphological overlap between the pathogenic E. histolytica, the commensal E. dispar and the amphizoic E. moshkovskii, necessitating molecular identification.  The present study developed a simple and economical 18S PCR-RFLP method for the simultaneous detection and differentiation of the three species.  Analysis indicated that the PCR-RFLP could successfully differentiate between the three species and was confirmed by sequence analysis. 
PCR-RFLP