Development and validation of SYBR Green- and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virus.Schmallenberg virus (SBV), discovered in Germany in 2011, causes congenital malformations in ruminants.  For SYBR Green-based detection of the S segment, the R2 value and efficiency of the developed assay were 0.99 and 99%, respectively. 
SYBR Green
Development and validation of SYBR Green- and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virus.Schmallenberg virus (SBV), discovered in Germany in 2011, causes congenital malformations in ruminants. 
probe-based reverse-transcription real-time PCR
Reverse-transcription real-time PCR (RT-rtPCR) assays based on various segments of SBV have been developed for molecular detection.  We developed alternative RT-rtPCR assays for SBV detection to avoid earlier reported mutations and hypervariable regions of the S and M segments of the viral genome.  Detection limits of the RT-rtPCR assays with new primer sets were 102 and 101 copies/µL for the S and M segments, respectively. 
RT-rtPCR
Development and validation of SYBR Green- and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virus.Schmallenberg virus (SBV), discovered in Germany in 2011, causes congenital malformations in ruminants.  Reverse-transcription real-time PCR (RT-rtPCR) assays based on various segments of SBV have been developed for molecular detection.  We developed alternative RT-rtPCR assays for SBV detection to avoid earlier reported mutations and hypervariable regions of the S and M segments of the viral genome.  Our assays should be suitable for SBV detection in ruminants and for in vitro studies of various SBV strains.
SBV
Development and validation of SYBR Green- and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virus.Schmallenberg virus (SBV), discovered in Germany in 2011, causes congenital malformations in ruminants. 
Schmallenberg virus