CCK-8 colorimetric method was used to detect cell proliferation percentage. 
CCK-8
The enzyme-linked immunosorbent assay (ELISA) was used to determine the content of tumor necrosis factor alpha (TNF-α), macrophage inflammatory protein-2 (MIP-2) and interleukin-10 (IL-10) in the supernatant. 
ELISA
Western blot was used to examine the expression levels of phosphorylated AKT (p-AKT) and total AKT protein. 
Western blot