Method: The gene expression (RNASeq) data of 375 patients with localized, locally advanced, and metastatic gastric cancer were extracted from The Cancer Genome Atlas. 
RNASeq
The Kruskal-Wallis test, Wilcoxon signed-rank test, and logistic regression were used to analyze the relationship between the clinicopathological characteristics and LINC01094 expression. 
Wilcoxon signed-rank test
The Kruskal-Wallis test, Wilcoxon signed-rank test, and logistic regression were used to analyze the relationship between the clinicopathological characteristics and LINC01094 expression. 
logistic regression
Cox regression analysis and the Kaplan-Meier method were used to assess prognostic factors of gastric cancer.  Result: LINC01094 expression was upregulated in gastric cancer tissues and strongly associated with overall survival using univariate Cox regression (hazard ratio [HR]  =  1.476, 95% CI  =  1.060-2.054, P  =  .021) and multivariate Cox regression analysis (HR  =  1.535, 95% CI  =  1.021-2.308, P  =  .039). 
Cox regression analysis
Cox regression analysis and the Kaplan-Meier method were used to assess prognostic factors of gastric cancer. 
Kaplan-Meier method
A nomogram based on Cox multivariate analysis was used to predict the impact of LINC01094 on gastric cancer prognosis. 
nomogram
Gene set enrichment analysis (GSEA) was used to identify key LINC01094-associated signaling pathways. 
Gene set enrichment analysis (GSEA)
Fluorescence in situ hybridization (FISH) was performed to detect the location of LINC01094 in the tissue, and a competing endogenous (ce)RNA network was constructed to identify LINC01094-related genes. 
Fluorescence in situ hybridization (FISH)