Material and Methods: We studied the EDS using high-resolution synchrotron phase contrast non-invasive imaging (SR-PCI), and micro-computed tomography (micro-CT).  Ten fresh human temporal bones underwent SR-PCI. 
SR-PCI
A Synchrotron and Micro-CT Study of the Human Endolymphatic Duct System: Is Meniere's Disease Caused by an Acute Endolymph Backflow? Material and Methods: We studied the EDS using high-resolution synchrotron phase contrast non-invasive imaging (SR-PCI), and micro-computed tomography (micro-CT).  One bone underwent micro-CT after fixation and staining with Lugol's iodine solution (I2KI) to increase tissue resolution.  The reunion duct was displayed with micro-CT. 
micro-CT
Data were processed using volume-rendering software to create 3D reconstructions allowing orthogonal sectioning, cropping, and tissue segmentation. 
volume-rendering software