EPB41L4A-AS1 and UNC5B-AS1 have diagnostic and prognostic significance in osteosarcoma.BACKGROUND
 This study explored the diagnostic and prognostic significance of EPB41L4A-AS1 and UNC5B-AS1 in osteosarcoma.


 Relative levels of EPB41L4A-AS1 and UNC5B-AS1 were detected in osteosarcoma tissue samples and cells.  The bioinformatics approach was used to identify targeting miRNA for EPB41L4A-AS1 and UNC5B-AS1.  In cell culture experiments, the influence of EPB41L4A-AS1 and UNC5B-AS1 on proliferation, migration, and invasion of the osteosarcoma cell line was examined by CCK-8 and Transwell assays.


 Levels of EPB41L4A-AS1 and UNC5B-AS1 were upregulated in osteosarcoma patients and cells compared with the healthy participants and normal cell lines.  EPB41L4A-AS1 and UNC5B-AS1 have a potent ability to distinguish the patients with osteosarcoma from the health.  EPB41L4A-AS1 and UNC5B-AS1 levels correlated with SSS stage.  Patients with high levels of EPB41L4A-AS1 and UNC5B-AS1 had significantly shorter survival times.  EPB41L4A-AS1 and UNC5B-AS1 were independent prognostic indexes for overall survival.  miR-1306-5p was a common target for EPB41L4A-AS1 and UNC5B-AS1.  A propulsive impact on cell proliferation, migration, and invasion by EPB41L4A-AS1 and UNC5B-AS1 was observed, but can be rescued by miR-1306-5p.


 It was concluded that upregulations of EPB41L4A-AS1 and UNC5B-AS1 expression were diagnostic and prognostic biomarkers for human osteosarcoma.  EPB41L4A-AS1 and UNC5B-AS1 contribute to the biological behavior of osteosarcoma via miR-1306-5p.
EPB41L4A-AS1
EPB41L4A-AS1 and UNC5B-AS1 have diagnostic and prognostic significance in osteosarcoma.BACKGROUND
 This study explored the diagnostic and prognostic significance of EPB41L4A-AS1 and UNC5B-AS1 in osteosarcoma.


 Relative levels of EPB41L4A-AS1 and UNC5B-AS1 were detected in osteosarcoma tissue samples and cells.  The bioinformatics approach was used to identify targeting miRNA for EPB41L4A-AS1 and UNC5B-AS1.  In cell culture experiments, the influence of EPB41L4A-AS1 and UNC5B-AS1 on proliferation, migration, and invasion of the osteosarcoma cell line was examined by CCK-8 and Transwell assays.


 Levels of EPB41L4A-AS1 and UNC5B-AS1 were upregulated in osteosarcoma patients and cells compared with the healthy participants and normal cell lines.  EPB41L4A-AS1 and UNC5B-AS1 have a potent ability to distinguish the patients with osteosarcoma from the health.  EPB41L4A-AS1 and UNC5B-AS1 levels correlated with SSS stage.  Patients with high levels of EPB41L4A-AS1 and UNC5B-AS1 had significantly shorter survival times.  EPB41L4A-AS1 and UNC5B-AS1 were independent prognostic indexes for overall survival.  miR-1306-5p was a common target for EPB41L4A-AS1 and UNC5B-AS1.  A propulsive impact on cell proliferation, migration, and invasion by EPB41L4A-AS1 and UNC5B-AS1 was observed, but can be rescued by miR-1306-5p.


 It was concluded that upregulations of EPB41L4A-AS1 and UNC5B-AS1 expression were diagnostic and prognostic biomarkers for human osteosarcoma.  EPB41L4A-AS1 and UNC5B-AS1 contribute to the biological behavior of osteosarcoma via miR-1306-5p.
UNC5B-AS1
miR-1306-5p was a common target for EPB41L4A-AS1 and UNC5B-AS1.  A propulsive impact on cell proliferation, migration, and invasion by EPB41L4A-AS1 and UNC5B-AS1 was observed, but can be rescued by miR-1306-5p.


 EPB41L4A-AS1 and UNC5B-AS1 contribute to the biological behavior of osteosarcoma via miR-1306-5p.
miR-1306-5p