The puncture resulting from cargo injection self-heals prior to ultraviolet (UV) curing.  UV curing and lyophilization were shown to enhance the stability of the polybubbles. 
UV
Synthesized poly(ε-caprolactone) and poly(ε-caprolactone) triacrylate were used to form stationary bubbles within an aqueous solution of 10% carboxymethylcellulose. 
poly(ε-caprolactone)
Synthesized poly(ε-caprolactone) and poly(ε-caprolactone) triacrylate were used to form stationary bubbles within an aqueous solution of 10% carboxymethylcellulose. 
poly(ε-caprolactone) triacrylate
Synthesized poly(ε-caprolactone) and poly(ε-caprolactone) triacrylate were used to form stationary bubbles within an aqueous solution of 10% carboxymethylcellulose. 
carboxymethylcellulose
BSA- CF 488 and HIV1 gp120/41 were used as the antigen in the study as a proof-of-concept. 
BSA-CF 488
BSA- CF 488 and HIV1 gp120/41 were used as the antigen in the study as a proof-of-concept. 
HIV1 gp120/41