Yeast transformation efficiency is enhanced by TORC1- and eisosome-dependent signaling.Transformation of baker's yeast (Saccharomyces cerevisiae) plays a key role in several experimental techniques, yet the molecular mechanisms underpinning transformation are still unclear.  Here, we show that target of rapamycin complex 1 (TORC1) activated by amino acids enhances transformation via ubiquitin-mediated endocytosis.  We created mutants of the TORC1 pathway, alpha-arrestins, and eisosome-related genes.  Our results demonstrate that the TORC1-Npr1-Art1/Rsp5 pathway regulates yeast transformation. 
TORC1
Yeast transformation efficiency is enhanced by TORC1- and eisosome-dependent signaling.Transformation of baker's yeast (Saccharomyces cerevisiae) plays a key role in several experimental techniques, yet the molecular mechanisms underpinning transformation are still unclear.  We created mutants of the TORC1 pathway, alpha-arrestins, and eisosome-related genes.  Additionally, we suggest DNA may be taken up by domains at the membrane compartment of Can1 (MCC) in the plasma membrane formed by eisosomes. 
eisosome
Our results demonstrate that the TORC1-Npr1-Art1/Rsp5 pathway regulates yeast transformation. 
TORC1-Npr1-Art1/Rsp5 pathway