Detection of Cytokine-Secreting Cells by Enzyme-Linked Immunospot (ELISpot). The enzyme-linked immunospot (ELISpot) is a highly sensitive immunoassay that measures the frequency of cytokine-secreting cells at the single-cell level.  The ELISpot assay is carried out in a 96-well plate and an automated ELISpot reader is used for analysis. 
ELISpot
The ELISpot assay is carried out in a 96-well plate and an automated ELISpot reader is used for analysis. 
ELISpot assay
The secreted molecules are detected by using a detection antibody system similar to that used in the enzyme-linked immunosorbent assay (ELISA). 
ELISA
The ELISpot assay is carried out in a 96-well plate and an automated ELISpot reader is used for analysis. 
ELISpot reader