Combined transcriptomics-metabolomics profiling of the heat shock response in the hyperthermophilic archaeon Pyrococcus furiosus.Pyrococcus furiosus is a remarkable archaeon able to grow at temperatures around 100 °C.  To gain insight into how this model hyperthermophile copes with heat stress, we compared transcriptomic and metabolomic data of cells subjected to a temperature shift from 90 °C to 97 °C.  The level of di-myo-inositol phosphate, a canonical heat stress solute among marine hyperthermophiles, increased considerably (5.4-fold) at elevated temperature.  This work provides the first metabolomic analysis of the heat shock response of a hyperthermophile.
THERM