The separation was carried out at room temperature by using Thermo Scientific ODS Hypersil C18 (250×4.6mm, 5µm) and mobile phase 70:30 (%v/v) mixture of ACN / H2O, 1.2 ml/min flow rate, detection on 253nm wavelength by PDA detector with run time of about 7 mins.  The method was also precise, accurate, robust and rugged and showed specificity in all applied stress conditions i.e. photolytic (200-800nm, 3h) thermal (70°C, 2h), oxidative (3% H2O2 , 70°C, 1h), acidic (0.1 N, 70°C, 1h), and basic (0.1 N, 60°C, 1h). 
THERM