The purification, identification and bioactivity study of a novel calcium-binding peptide from casein hydrolysate.In this study, a novel calcium-binding peptide from casein hydrolysate was purified using reversed-phase high performance liquid chromatography and sequenced by high-performance liquid chromatography-mass spectrometry (MS)/MS. 
reversed-phase high performance liquid chromatography
The purification, identification and bioactivity study of a novel calcium-binding peptide from casein hydrolysate.In this study, a novel calcium-binding peptide from casein hydrolysate was purified using reversed-phase high performance liquid chromatography and sequenced by high-performance liquid chromatography-mass spectrometry (MS)/MS. 
high-performance liquid chromatography-mass spectrometry (MS)/MS
The calcium binding characteristics of VLPVPQK were further investigated using UV absorption spectroscopy, zeta potential and isothermal titration calorimetry (ITC). 
UV absorption spectroscopy
The calcium binding characteristics of VLPVPQK were further investigated using UV absorption spectroscopy, zeta potential and isothermal titration calorimetry (ITC). 
zeta potential
The calcium binding characteristics of VLPVPQK were further investigated using UV absorption spectroscopy, zeta potential and isothermal titration calorimetry (ITC). 
isothermal titration calorimetry (ITC)