Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in Caenorhabditis elegans.Autophagy is being studied intensively in Caenorhabditis elegans in the context of protein homeostasis and aging.  Our new protocol for Western blot analysis is applicable for both the detection of transgenic and endogenous LGG-1 proteins and provides a quantifiable method to assess autophagic flux. 
Western blot analysis
However, in contrast to the yeast and mammalian autophagosomal membrane proteins Atg8 and LC3, lipidation of the C. elegans ortholog LGG-1 with phosphatidylethanolamine has rarely been investigated by western blotting. 
Western blotting
We attribute this shortcoming to technical problems with separating the nonlipidated from the lipidated LGG-1 protein by gel electrophoresis. 
gel electrophoresis