HPLC-MS/MS was used to assess the concentration of anlotinib in blood and brain samples. 
HPLC-MS/MS
Western blotting, immunohistochemistry, and immunofluorescent staining were performed to determine the underlying mechanism.



Western blotting
Western blotting, immunohistochemistry, and immunofluorescent staining were performed to determine the underlying mechanism.



Immunohistochemistry
Western blotting, immunohistochemistry, and immunofluorescent staining were performed to determine the underlying mechanism.



Immunofluorescent staining