In this study, Western blot was used to detect cytoplasmic and nuclear protein of p53, total protein of AMP-activated protein kinase (AMPK), mammalian target of rapamycin (mTOR) and Light chain 3 (LC3). 
Western blot
Quantitative Real-Time PCR (qRT-PCR) was used to detect p53 mRNA level after treated with IL-7. 
Quantitative Real-Time PCR (qRT-PCR)
Then using transmission electron microscopy to observe the morphological change of autophagosome. 
Transmission electron microscopy
123 cases of NSCLC were collected for survival analysis, immunohistochemistry staining and cox regression multivariate analysis. 
Immunohistochemistry staining