Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function.Tunicates are marine, non-vertebrate chordates that comprise the sister group to the vertebrates.  Here, we take advantage of the low-cost and simplicity of Ciona by using tissue-specific CRISPR/Cas9-mediated mutagenesis to screen for genes potentially involved in mechanosensation and metamorphosis, in the context of an undergraduate "capstone" research course. 
CRISPR/Cas9
Using CRISPR/Cas9 to identify genes required for mechanosensory neuron development and function.Tunicates are marine, non-vertebrate chordates that comprise the sister group to the vertebrates.  Here, we take advantage of the low-cost and simplicity of Ciona by using tissue-specific CRISPR/Cas9-mediated mutagenesis to screen for genes potentially involved in mechanosensation and metamorphosis, in the context of an undergraduate "capstone" research course. 
CRISPR/Cas9