Lysine long-chain fatty acylation regulates the TEAD transcription factor.TEAD transcription factors are responsible for the transcriptional output of Hippo signaling.  TEAD activity is primarily regulated by phosphorylation of its coactivators, YAP and TAZ.  In addition, cysteine palmitoylation has recently been shown to regulate TEAD activity.  Here, we report lysine long-chain fatty acylation as a posttranslational modification of TEADs.  Lysine fatty acylation, like cysteine palmitoylation, contributes to the transcriptional activity of TEADs by enhancing the interaction with YAP and TAZ, but it is more stable than cysteine acylation, suggesting that the lysine fatty-acylated TEAD acts as a "stable active form."  Significantly, lysine fatty acylation of TEAD increased upon Hippo signaling activation despite a decrease in cysteine acylation.  Our results provide insight into the role of fatty-acyl modifications in the regulation of TEAD activity.
TEAD
TEAD activity is primarily regulated by phosphorylation of its coactivators, YAP and TAZ.  Lysine fatty acylation, like cysteine palmitoylation, contributes to the transcriptional activity of TEADs by enhancing the interaction with YAP and TAZ, but it is more stable than cysteine acylation, suggesting that the lysine fatty-acylated TEAD acts as a "stable active form." 
YAP
TEAD activity is primarily regulated by phosphorylation of its coactivators, YAP and TAZ.  Lysine fatty acylation, like cysteine palmitoylation, contributes to the transcriptional activity of TEADs by enhancing the interaction with YAP and TAZ, but it is more stable than cysteine acylation, suggesting that the lysine fatty-acylated TEAD acts as a "stable active form." 
TAZ
Lysine long-chain fatty acylation regulates the TEAD transcription factor.TEAD transcription factors are responsible for the transcriptional output of Hippo signaling.  Significantly, lysine fatty acylation of TEAD increased upon Hippo signaling activation despite a decrease in cysteine acylation. 
Hippo