Development and validation of a highly sensitive HPLC-MS/MS method for the QAP14, a novel potential anti-cancer agent, in rat plasma and its application to a pharmacokinetic study.QAP14 is a novel anti-cancer compound exhibiting good efficacy and safety in preclinical study.  To investigate its pharmacokinetic properties, a rapid and sensitive HPLC-MS/MS method was developed and validated to quantify the concentration of QAP14 in rat plasma. 
HPLC-MS/MS
QAP14 was separated on ZORBAX Eclipse XDB-C8 column with a gradient elution. 
ZORBAX Eclipse XDB-C8 column
Erlotinib was selected as internal standard and plasma samples were prepared by precipitation with acetonitrile. 
acetonitrile