Application of metagenomic next-generation sequencing for bronchoalveolar lavage diagnostics in patients with lower respiratory tract infections.OBJECTIVE
 Metagenomic next-generation sequencing (mNGS) has the potential to overcome the shortcomings of traditional culture methods. 
metagenomic next-generation sequencing
Metagenomic next-generation sequencing (mNGS) has the potential to overcome the shortcomings of traditional culture methods.  This study aimed to assess the diagnostic value of mNGS in patients with lower respiratory tract infections (LRTIs).


 Pathogens in bronchoalveolar lavage fluid (BALF) samples were investigated to compare diagnoses by mNGS with culture methods.


 Compared with culture methods, mNGS had a diagnostic sensitivity of 80% and a specificity of 35.13% with an agreement rate of 44.68% between these two methods.  mNGS significantly increased the pathogen detection rate.


 mNGS may show some advantages in identifying a wide range of LRTI pathogens, improving the sensitivity for viruses and atypical pathogens. 
mNGS
Application of metagenomic next-generation sequencing for bronchoalveolar lavage diagnostics in patients with lower respiratory tract infections.OBJECTIVE
 Metagenomic next-generation sequencing (mNGS) has the potential to overcome the shortcomings of traditional culture methods. 
next-generation sequencing