GSE59247 dataset was downloaded from gene expression omnibus (GEO) database and analyzed with GEO2R software. 
GEO2R
The differential miRNA expressions in BC cells were evaluated by miRNome PCR array. 
miRNome PCR array
Venn diagram was used to reveal co-differentially expressed miRNAs between GSE59247 dataset and miRNome array.  Venn diagram analysis showed overlap of 11 miRNAs from in silico and in vitro analysis. 
Venn diagram
KEGG pathway enrichment analysis was performed to find miRNA targets and results were validated by TNM plot analysis and q-RT-PCR. 
KEGG pathway enrichment analysis
KEGG pathway enrichment analysis was performed to find miRNA targets and results were validated by TNM plot analysis and q-RT-PCR. 
TNM plot analysis
KEGG pathway enrichment analysis was performed to find miRNA targets and results were validated by TNM plot analysis and q-RT-PCR.  q-RT-PCR results confirmed that Lysine degradation pathway related genes SETD7, SETDB2, EHHADH, SETMAR, KMT2A and SUV39H2 were differentially expressed in BC cells. 
q-RT-PCR
TargetScan database was used to predict the association of miRNAs and 3'-untranslated regions of target genes and their expressions were visualized by human protein atlas database. 
TargetScan database
TargetScan database was used to predict the association of miRNAs and 3'-untranslated regions of target genes and their expressions were visualized by human protein atlas database. 
Human protein atlas database