Here, we created a circular fluorescent probe-mediated isothermal amplification (CFPA) method. 
CFPA
This novel method uses two circular fluorescent probes and Bst DNA polymerase to construct an overlapping structure that can be cut off by flap structure-specific endonuclease 1, separating the fluorescence and quenching groups on the probes. 
Bst DNA polymerase
This novel method uses two circular fluorescent probes and Bst DNA polymerase to construct an overlapping structure that can be cut off by flap structure-specific endonuclease 1, separating the fluorescence and quenching groups on the probes. 
flap structure-specific endonuclease 1
Here, we created a circular fluorescent probe-mediated isothermal amplification (CFPA) method. 
CFPA