PACmn for improved optogenetic control of intracellular cAMP.BACKGROUND
 The plasma membrane-anchored PAC (PACmn) had an undetectable dark activity which increased >4000-fold in the light.  PACmn does not raise resting cAMP nor, when expressed in hippocampal neurons, affect cAMP-dependent kinase (PKA) activity in the dark, but rapidly and reversibly increases cAMP and PKA activity in the soma and dendrites upon illumination.  PACmn is a valuable optogenetic tool for precise cell-autonomous and transient stimulation of cAMP signaling pathways in diverse cell types.
PACmn