Western blotting was performed to detect PRMT8, GDNF, cystine/glutamate transporter XCT, glutathione peroxidase 4, 4-hydroxynonenal, heme oxygenase-1, inducible nitric oxide synthase (iNOS), CD16, and arginase 1 (Arg1). 
Western blotting
ELISA was performed to measure the expression levels of IL-6, IL-1β, and TNF-α. 
ELISA
Flow cytometry and immunofluorescence were used to assess macrophage polarization. 
Flow cytometry
Transmission electron microscopy was used to reveal the morphological characteristics of mitochondria. 
Transmission electron microscopy
Basso, Beattie, and Bresnahan score, hematoxylin and eosin staining, and immunofluorescence were used, respectively, to detect changes in rat locomotion, spinal cord histopathology, and NeuN expression in the spinal cord.  The expression levels of iNOS and Arg1 in the spinal cord were visualized by immunofluorescence.  Flow cytometry and immunofluorescence were used to assess macrophage polarization. 
Immunofluorescence
Chromatin immunoprecipitation was used to identify the binding of PRMT8 to the GDFN promoter.



Chromatin immunoprecipitation