PacBio Iso-Seq Improves the Rainbow Trout Genome Annotation and Identifies Alternative Splicing Associated With Economically Important Phenotypes.Rainbow trout is an important model organism that has received concerted international efforts to study the transcriptome. 
PacBio Iso-Seq
This study reported a first full-length transcriptome assembly of the rainbow trout using single-molecule long-read isoform sequencing (Iso-Seq). 
single-molecule long-read isoform sequencing (Iso-Seq)
Extensive computational approaches were used to refine and validate the reconstructed transcriptome. 
computational approaches
The study identified 10,640 high-confidence transcripts not previously annotated, in addition to 1,479 isoforms not mapped to the current Swanson reference genome. 
Swanson reference genome