Enzyme-linked immunosorbent assays (ELISAs) have shown that urinary titin is a useful noninvasive biomarker for the diagnosis and screening of not only DMD, but also of neuromuscular diseases, for predicting the outcome of cardiomyopathy and for evaluating physical activities.  The development of ELISA systems to measure urinary titin has opened a door to studying muscle degradation directly and noninvasively. 
ELISAs
Enzyme-linked immunosorbent assays (ELISAs) have shown that urinary titin is a useful noninvasive biomarker for the diagnosis and screening of not only DMD, but also of neuromuscular diseases, for predicting the outcome of cardiomyopathy and for evaluating physical activities.  The development of ELISA systems to measure urinary titin has opened a door to studying muscle degradation directly and noninvasively. 
ELISA