Serum estradiol was measured using a Sciex Triple QuadTM 6500+ UHPLC-MS/MS (Sciex, Framingham, MA, USA). 
Sciex Triple QuadTM 6500+
Statistical analysis was performed using MedCalc (MedCalc, Ostend, Belgium) and Analyse-it (Analyse-it Software Ltd., Leeds, United Kingdom) software.



MedCalc
Statistical analysis was performed using MedCalc (MedCalc, Ostend, Belgium) and Analyse-it (Analyse-it Software Ltd., Leeds, United Kingdom) software.



Analyse-it Software Ltd.