Developing an improved UHPLC method for impurity profile analysis of ceftriaxone using analytical quality by design.In this study, we developed a new reversed-phase ultra-high-performance liquid chromatography (UHPLC) method for comprehensively measuring impurities in ceftriaxone.  Additionally, the developed UHPLC method could be transferred back to HPLC in a single step using a Columns Calculator, providing a new approach for the rapid and effective development of the HPLC method. 
UHPLC
The method was developed based on the Chinese Pharmacopoeia (ChP) HPLC method, which is limited by the lack of selectivity to potential impurities and a long running time. 
the Chinese Pharmacopoeia (ChP) HPLC method
Further optimisation and Monte-Carlo simulations were performed to map out the design space. 
Monte-Carlo simulations
Additionally, the developed UHPLC method could be transferred back to HPLC in a single step using a Columns Calculator, providing a new approach for the rapid and effective development of the HPLC method. 
Columns Calculator