Our method includes using "Enzymer," a software for designing RNA sequences with desired secondary structures that may include pseudoknots.  Enzymer implements an efficient stochastic search and optimization algorithm to sample RNA sequences from low ensemble defect mutational landscape of an initial design template to generate an RNA sequence that is predicted to fold into the desired target structure.
Enzymer
Our method includes using "Enzymer," a software for designing RNA sequences with desired secondary structures that may include pseudoknots.  Enzymer implements an efficient stochastic search and optimization algorithm to sample RNA sequences from low ensemble defect mutational landscape of an initial design template to generate an RNA sequence that is predicted to fold into the desired target structure.
Enzymer