Reproducibility of novel immune-inflammatory biomarkers over 4 months: an analysis with repeated measures design.Aim: Assessment of the feasibility and reliability of immune-inflammatory biomarker measurements.  Methods: The following biomarkers were assessed in 207 predominantly healthy participants at baseline and after 4 months: MMF, TGF-β, suPAR and clusterin.  Results: Intraclass correlation coefficients (95% CIs) ranged from good for TGF-β (0.75 [95% CI: 0.33-0.90]) to excellent for MMF (0.81 [95% CI: 0.64-0.90]), clusterin (0.83 [95% CI: 0.78-0.87]) and suPAR (0.91 [95% CI: 0.88-0.93]).  Measurement of TGF-β was challenged by the large number of values below the detection limit.  Conclusion: Single measurements of suPAR, clusterin and MMF could serve as feasible and reliable biomarkers of immune-inflammatory pathways in biomedical research.
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