This study investigated the presence of BTV and EHDV among cattle which had been found BTV-seropositive by ELISA method.  Ninety-four of the 227 animals tested by a BTV ELISA were positive for BTV antibodies (41.4%).  Of these 94 ELISA-positive cattle, only 83 EDTA-blood samples were available and therefore tested for BTV and EHDV genome detection by RT-PCR and sequencing. 
ELISA
Of these 94 ELISA-positive cattle, only 83 EDTA-blood samples were available and therefore tested for BTV and EHDV genome detection by RT-PCR and sequencing.  Of the cattle sampled in 2016, results revealed that two were RT-PCR-positive for BTV and seven for EHDV. 
RT-PCR
Of these 94 ELISA-positive cattle, only 83 EDTA-blood samples were available and therefore tested for BTV and EHDV genome detection by RT-PCR and sequencing.  Sequencing showed the presence of EHDV-1 and BTV-3 genome sequences. 
sequencing
Of the 66 blood samples tested following their collection in 2017, they were all EHDV-negative by RT-qPCR while five were BTV- positive by RT-qPCR. 
RT-qPCR