Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV.Human primary natural killer (NK) cells are being widely advanced for cancer immunotherapy.  Here, we developed a highly efficient method for site-specific gene insertion in NK cells using CRISPR (Cas9/RNP) and AAVs. 
CRISPR
Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV.Human primary natural killer (NK) cells are being widely advanced for cancer immunotherapy.  Here, we developed a highly efficient method for site-specific gene insertion in NK cells using CRISPR (Cas9/RNP) and AAVs.  We compared AAV vectors designed to mediate gene insertion by different DNA repair mechanisms, homology arm lengths, and virus concentrations. 
AAV
Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV.Human primary natural killer (NK) cells are being widely advanced for cancer immunotherapy.  We then validated the method for site-directed gene insertion of CD33-specific CARs into primary human NK cells.  CAR transduction was efficient, its expression remained stable after expansion, and it improved efficacy against AML targets.
CAR