The cell growth inhibition effect was monitored by the IncuCyte ZOOM Live-Cell Analysis System, and cell viability was determined by Cell Counting Kit-8 assays. 
IncuCyte ZOOM Live-Cell Analysis System
The cell growth inhibition effect was monitored by the IncuCyte ZOOM Live-Cell Analysis System, and cell viability was determined by Cell Counting Kit-8 assays. 
Cell Counting Kit-8 assays
Apoptosis was detected by Annexin V/Propidium Iodide double staining. 
Annexin V/Propidium Iodide double staining
Methylation-specific polymerase chain reaction (PCR) was applied to determine the methylation status of DR4. 
Methylation-specific polymerase chain reaction (PCR)
The messenger RNA (mRNA) and protein expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. 
quantitative real-time polymerase chain reaction (qRT-PCR)
The messenger RNA (mRNA) and protein expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. 
western blot
The expression of the DRs on the cell membrane surfaces was analyzed by flow cytometry.



flow cytometry