We devised a combinatorial imaging technique that combines deconvolution with stepwise optical saturation microscopy (DeSOS) to circumvent this issue and image cells in their native physiological environment.  Here, we provide an open-access application to obtain DeSOS images from conventional microscope images obtained at low excitation powers.  We show that DeSOS can be used in time-lapse imaging to generate super-resolution movies in zebrafish.  DeSOS was also validated in live mice. 
DeSOS
We devised a combinatorial imaging technique that combines deconvolution with stepwise optical saturation microscopy (DeSOS) to circumvent this issue and image cells in their native physiological environment.  Here, we provide an open-access application to obtain DeSOS images from conventional microscope images obtained at low excitation powers.  We show that DeSOS can be used in time-lapse imaging to generate super-resolution movies in zebrafish.  DeSOS was also validated in live mice. 
DeSOS