PZP knockout cells were generated utilizing the CRISPR-Cas9 approach in MCF7 and T47D (breast cancer) cell lines, and colony formation, cell proliferation, and migration assays carried out. 
CRISPR-Cas9
TGF-β and SMAD expression studies were performed using qRT-PCR and Western blot. 
qRT-PCR
TGF-β and SMAD expression studies were performed using qRT-PCR and Western blot. 
Western blot