(a) the steps required for the preparation of semisynthetic lipid vesicles (liposomes), including giant unilamellar vesicles, 
liposomes
(b) ultracentrifugation and fluorescence methods for assaying protein binding to membranes, and 
ultracentrifugation
(b) ultracentrifugation and fluorescence methods for assaying protein binding to membranes, and 
fluorescence
(a) the steps required for the preparation of semisynthetic lipid vesicles (liposomes), including giant unilamellar vesicles, 
lipid vesicles