A qPCR Method for AAV Genome Titer with ddPCR-Level of Accuracy and Precision.Recombinant adeno-associated virus (rAAV) is one of the main vectors used in gene therapy.  AAV genome titer is traditionally determined by qPCR; however, assay precision is not optimal despite extensive efforts.  However, currently ddPCR is not as widely available as qPCR and operates at a lower throughput and a higher cost.  In this paper, we introduce an improved qPCR method with two major optimizations: (1) using an AAV reference material as qPCR standard instead of plasmid DNA and (2) implementing a "digestion-free" method by adding 5% Tween 20 to standard and sample preparations. 
qPCR
A qPCR Method for AAV Genome Titer with ddPCR-Level of Accuracy and Precision.Recombinant adeno-associated virus (rAAV) is one of the main vectors used in gene therapy.  More recently, droplet digital PCR (ddPCR) emerged as a powerful alternative that offers excellent accuracy and precision.  However, currently ddPCR is not as widely available as qPCR and operates at a lower throughput and a higher cost. 
ddPCR