A genome-wide CRISPR functional survey of the human phagocytosis molecular machinery.Phagocytosis, the process by which cells engulf large particles, plays a vital role in driving tissue clearance and host defense.  To create a functional map in human cells, we performed a genome-wide CRISPRko FACS screen that identified 716 genes. 
CRISPR
To create a functional map in human cells, we performed a genome-wide CRISPRko FACS screen that identified 716 genes. 
FACS
Mapping those hits to a comprehensive protein-protein interaction network annotated for functional cellular processes allowed retrieval of protein complexes identified multiple times and detection of missing phagocytosis regulators. 
Protein-Protein Interaction Network