THRIFTY-A High-throughput Single Muscle Fiber Typing Method Based on Immunofluorescence Detection.Skeletal muscle consists of a mixture of fiber types with different functional and metabolic characteristics.  Here, we present the protocol for a new method, which we have named THRIFTY (high-THRoughput Immunofluorescence Fiber TYping), that enables rapid fiber type identification using antibodies towards the different myosin heavy chain (MyHC) isoforms of fast and slow twitch muscle fibers.  The THRIFTY protocol is approximately three times as fast as the dot blot method, which enables not only time-sensitive assays to be performed but also increases the feasibility to conduct large-scale investigations into fiber type specific physiology.  Graphical Overview Graphical overview of the THRIFTY workflow. 
THRIFTY
More recently, the introduction of the dot blot method significantly increased the speed of fiber typing.  The THRIFTY protocol is approximately three times as fast as the dot blot method, which enables not only time-sensitive assays to be performed but also increases the feasibility to conduct large-scale investigations into fiber type specific physiology. 
the dot blot method