Knocking out Analysis of the CpxP gene using Crispr/Cas9 in Escherichia coli MG1655.Based on the analysis of cpxP genes among Escherichia coli strains, cpxP gene-targeting short guide RNA (sgRNA) was designed and inserted into the pGL3-MGP-RNA. 
Crispr/Cas9
The donor sequences (MG-HR) for homologous repair were designed and cloned by PCR.  The cpxP gene expression cassette was amplified by PCR and subcloned into pBBR1MCS-2. 
PCR
Knocking out Analysis of the CpxP gene using Crispr/Cas9 in Escherichia coli MG1655.Based on the analysis of cpxP genes among Escherichia coli strains, cpxP gene-targeting short guide RNA (sgRNA) was designed and inserted into the pGL3-MGP-RNA.  MG-HR and pGL3-MGP-RNA were transformed into E. coli MG1655 (pCas9). 
pGL3-MGP-RNA
The donor sequences (MG-HR) for homologous repair were designed and cloned by PCR.  MG-HR and pGL3-MGP-RNA were transformed into E. coli MG1655 (pCas9). 
MG-HR
The cpxP gene expression cassette was amplified by PCR and subcloned into pBBR1MCS-2. 
pBBR1MCS-2