Biosynthesis of Low Molecular Weight Antifungal Protein from Aspergillus giganteus in Batch Fermentation and In-Vitro Assay.In present study, Taguchi's design of experiment L9 orthogonal array was created using Qualitek-4 software with four most critical factors namely, K2HPO4, MgSO4, CaCl2 and culture pH. Production of a new intracellular antifungal protein in submerged fermentation was optimized with yield of 0.98±0.1 mg/gram dry cell weight mycelia from Aspergillus giganteus MTCC 8408. 
Qualitek-4
The average molecular mass of the purified protein was figured as 5.122 kDa using Electro Spray Ionization-Mass Spectrometry. 
Electro Spray Ionization-Mass Spectrometry
Scanning electron microscopy was used to correlate the effect of selected factors on fungal cell morphology and its metabolite production. 
Scanning electron microscopy