Cell proliferation was measured by CCK-8 assay and cell colony formation. 
CCK-8 assay
Cell cycle and apoptosis was analyzed by flow cytometry. 
flow cytometry
The existence of mTOR signaling pathway was demonstrated by immunocytochemistry. 
immunocytochemistry
Western-blot and real time-PCR were used to test whether mTOR-signaling pathway was inhibited with rapamycin treatment.



Western-blot
Western-blot and real time-PCR were used to test whether mTOR-signaling pathway was inhibited with rapamycin treatment.



real time-PCR