%MinMax: A versatile tool for calculating and comparing synonymous codon usage and its impact on protein folding.Most amino acids can be encoded by more than one synonymous codon, but these are rarely used with equal frequency.  Here we describe the core functions and new features of %MinMax, a codon usage calculator freely available as a web-based portal and downloadable script (http://www.codons.org).  %MinMax evaluates the relative usage frequencies of the synonymous codons used to encode a protein sequence of interest and compares these results to a rigorous null model.  Crucially, for analyzing codon usage in common host organisms %MinMax requires only the coding sequence as input; with a user-input codon frequency table, %MinMax can be used to evaluate synonymous codon usage patterns for any coding sequence from any fully sequenced genome.  %MinMax makes no assumptions regarding the impact of transfer ribonucleic acid concentrations or other molecular-level interactions on translation rates, yet its output is sufficient to predict the effects of synonymous codon substitutions on cotranslational folding mechanisms.  A simple calculation included within %MinMax can be used to harmonize codon usage frequencies for heterologous gene expression.
%MinMax
%MinMax: A versatile tool for calculating and comparing synonymous codon usage and its impact on protein folding.Most amino acids can be encoded by more than one synonymous codon, but these are rarely used with equal frequency.  Here we describe the core functions and new features of %MinMax, a codon usage calculator freely available as a web-based portal and downloadable script (http://www.codons.org).  %MinMax evaluates the relative usage frequencies of the synonymous codons used to encode a protein sequence of interest and compares these results to a rigorous null model.  Crucially, for analyzing codon usage in common host organisms %MinMax requires only the coding sequence as input; with a user-input codon frequency table, %MinMax can be used to evaluate synonymous codon usage patterns for any coding sequence from any fully sequenced genome.  %MinMax makes no assumptions regarding the impact of transfer ribonucleic acid concentrations or other molecular-level interactions on translation rates, yet its output is sufficient to predict the effects of synonymous codon substitutions on cotranslational folding mechanisms.  A simple calculation included within %MinMax can be used to harmonize codon usage frequencies for heterologous gene expression.
%MinMax
%MinMax: A versatile tool for calculating and comparing synonymous codon usage and its impact on protein folding.Most amino acids can be encoded by more than one synonymous codon, but these are rarely used with equal frequency.  Here we describe the core functions and new features of %MinMax, a codon usage calculator freely available as a web-based portal and downloadable script (http://www.codons.org).  %MinMax evaluates the relative usage frequencies of the synonymous codons used to encode a protein sequence of interest and compares these results to a rigorous null model.  Crucially, for analyzing codon usage in common host organisms %MinMax requires only the coding sequence as input; with a user-input codon frequency table, %MinMax can be used to evaluate synonymous codon usage patterns for any coding sequence from any fully sequenced genome.  %MinMax makes no assumptions regarding the impact of transfer ribonucleic acid concentrations or other molecular-level interactions on translation rates, yet its output is sufficient to predict the effects of synonymous codon substitutions on cotranslational folding mechanisms.  A simple calculation included within %MinMax can be used to harmonize codon usage frequencies for heterologous gene expression.
%MinMax
%MinMax: A versatile tool for calculating and comparing synonymous codon usage and its impact on protein folding.Most amino acids can be encoded by more than one synonymous codon, but these are rarely used with equal frequency.  Here we describe the core functions and new features of %MinMax, a codon usage calculator freely available as a web-based portal and downloadable script (http://www.codons.org).  %MinMax evaluates the relative usage frequencies of the synonymous codons used to encode a protein sequence of interest and compares these results to a rigorous null model.  Crucially, for analyzing codon usage in common host organisms %MinMax requires only the coding sequence as input; with a user-input codon frequency table, %MinMax can be used to evaluate synonymous codon usage patterns for any coding sequence from any fully sequenced genome.  %MinMax makes no assumptions regarding the impact of transfer ribonucleic acid concentrations or other molecular-level interactions on translation rates, yet its output is sufficient to predict the effects of synonymous codon substitutions on cotranslational folding mechanisms.  A simple calculation included within %MinMax can be used to harmonize codon usage frequencies for heterologous gene expression.
%MinMax
%MinMax: A versatile tool for calculating and comparing synonymous codon usage and its impact on protein folding.Most amino acids can be encoded by more than one synonymous codon, but these are rarely used with equal frequency.  Here we describe the core functions and new features of %MinMax, a codon usage calculator freely available as a web-based portal and downloadable script (http://www.codons.org).  %MinMax evaluates the relative usage frequencies of the synonymous codons used to encode a protein sequence of interest and compares these results to a rigorous null model.  Crucially, for analyzing codon usage in common host organisms %MinMax requires only the coding sequence as input; with a user-input codon frequency table, %MinMax can be used to evaluate synonymous codon usage patterns for any coding sequence from any fully sequenced genome.  %MinMax makes no assumptions regarding the impact of transfer ribonucleic acid concentrations or other molecular-level interactions on translation rates, yet its output is sufficient to predict the effects of synonymous codon substitutions on cotranslational folding mechanisms.  A simple calculation included within %MinMax can be used to harmonize codon usage frequencies for heterologous gene expression.
%MinMax