Comparison of quantitative methods based on SYBR Green real-time qPCR to estimate pork meat adulteration in processed beef products.Quantitative real-time PCR (qPCR) is a modern technique that has been widely used for the detection of species used in meat products.  Binary pork-beef mixtures were used to construct standard curves of SYBR Green-based qPCR products using five quantification approaches, and they were validated and compared using in-house pork models. 
SYBR Green
Comparison of quantitative methods based on SYBR Green real-time qPCR to estimate pork meat adulteration in processed beef products.Quantitative real-time PCR (qPCR) is a modern technique that has been widely used for the detection of species used in meat products.  For obtaining accurate and reliable qPCR results, we assessed two common DNA quantification methods for isolated DNA and five quantification approaches for qPCR products.  DNA dilution based on spectrofluorometric results showed better qPCR results than those based on spectrophotometry in terms of linear correlation, amplification efficiency, and linear dynamic range.  Binary pork-beef mixtures were used to construct standard curves of SYBR Green-based qPCR products using five quantification approaches, and they were validated and compared using in-house pork models. 
qPCR
Binary pork-beef mixtures were used to construct standard curves of SYBR Green-based qPCR products using five quantification approaches, and they were validated and compared using in-house pork models. 
SYBR Green-based qPCR