Synthesis of new 4-anilinoquinazoline analogues and evaluation of their EGFR inhibitor activity.Thirteen of 4-anilinoquinazoline derivatives with imine groups at position 6 of quinazoline ring were synthesized and their antitumor activities were evaluated by MTT assay and Western blotting analysis.  The MTT assay was carried out on three human cancer cell lines (A549, HepG2 and SMMC7721) with EGFR highly expressed. 
MTT assay
Synthesis of new 4-anilinoquinazoline analogues and evaluation of their EGFR inhibitor activity.Thirteen of 4-anilinoquinazoline derivatives with imine groups at position 6 of quinazoline ring were synthesized and their antitumor activities were evaluated by MTT assay and Western blotting analysis.  Compounds 14 and 13j were chosen to perform Western blotting analysis on A549. 
Western blotting analysis