The amino acid sequences of all allergens of Glycine max and their isoallergens have been taken, and 3D structure of allergens (Gly m 3, Gly m 4, Gly m 5, Gly m 6 and Gly m 8) and their isoallergens were generated using Modeller v9.17. 
Modeller v9.17
The modeled structures were further validated using PSVS, ProSA, RAMPAGE, and PDBsum. 
PSVS
The modeled structures were further validated using PSVS, ProSA, RAMPAGE, and PDBsum. 
ProSA
The modeled structures were further validated using PSVS, ProSA, RAMPAGE, and PDBsum. 
RAMPAGE
The modeled structures were further validated using PSVS, ProSA, RAMPAGE, and PDBsum. 
PDBsum
HL domain of Fab region of human IgE (PDBID: 2R56) was generated using UCSFchimera. 
UCSFchimera
The HL domain was minimized by Schrodinger software using the OPLS_2005 force field. 
Schrodinger software
SiteMap identified epitope binding site of the minimized domain. 
SiteMap
All the predicted epitopes of different allergens were docked to the binding site of HL domain using the Patchdock server. 
Patchdock server