Updates on the web-based VIOLIN vaccine database and analysis system.The integrative Vaccine Investigation and Online Information Network (VIOLIN) vaccine research database and analysis system (http://www.violinet.org) curates, stores, analyses and integrates various vaccine-associated research data.  Starting from 211 vaccines annotated at the end of 2007, VIOLIN now includes over 3240 vaccines for 192 infectious diseases and eight noninfectious diseases (e.g. cancers and allergies).  Under the umbrella of VIOLIN, >10 relatively independent programs are developed.  VIOLIN also includes Vaxign, the first web-based vaccine candidate prediction program based on reverse vaccinology.  VIOLIN collects and analyzes different vaccine components including vaccine adjuvants (Vaxjo) and DNA vaccine plasmids (DNAVaxDB).  VIOLIN includes licensed human vaccines (Huvax) and veterinary vaccines (Vevax).  The Vaccine Ontology is applied to standardize and integrate various data in VIOLIN.  VIOLIN also hosts the Ontology of Vaccine Adverse Events (OVAE) that logically represents adverse events associated with licensed human vaccines.
VIOLIN
For example, Protegen stores over 800 protective antigens experimentally proven valid for vaccine development.  Specific patterns were identified from the genes collected in Protegen and VirmugenDB. 
Protegen
VirmugenDB annotated over 200 'virmugens', a term coined by us to represent those virulence factor genes that can be mutated to generate successful live attenuated vaccines.  Specific patterns were identified from the genes collected in Protegen and VirmugenDB. 
VirmugenDB
VIOLIN also includes Vaxign, the first web-based vaccine candidate prediction program based on reverse vaccinology. 
Vaxign
VIOLIN collects and analyzes different vaccine components including vaccine adjuvants (Vaxjo) and DNA vaccine plasmids (DNAVaxDB). 
Vaxjo
VIOLIN collects and analyzes different vaccine components including vaccine adjuvants (Vaxjo) and DNA vaccine plasmids (DNAVaxDB). 
DNAVaxDB
VIOLIN includes licensed human vaccines (Huvax) and veterinary vaccines (Vevax). 
Huvax
VIOLIN includes licensed human vaccines (Huvax) and veterinary vaccines (Vevax). 
Vevax
VIOLIN also hosts the Ontology of Vaccine Adverse Events (OVAE) that logically represents adverse events associated with licensed human vaccines.
Ontology of Vaccine Adverse Events (OVAE)
The Vaccine Ontology is applied to standardize and integrate various data in VIOLIN. 
Vaccine Ontology