Tuning the catalytic properties of lipases immobilized on divinylsulfone activated agarose by altering its nanoenvironment.Lipase from Thermomyces lanuginosus (TLL) and lipase B from Candida antarctica (CALB) have been immobilized on divinylsulfone (DVS) activated agarose beads at pH 10 for 72 h.  The blocking has generally positive effects on enzyme stability in both thermal and organic solvent inactivations.  For example, CALB improved 7.5-fold the thermal stability after blocking with imidazole. 
THERM