A total of 418 Han Chinese (249 overweight/obese and 169 healthy control subjects) in the Chengdu area were studied using PCR-RFLP analysis. 
PCR-RFLP
Total serum cholesterol (TC) and triglycerides (TGs) were measured using an enzymatic method. 
enzymatic method
High density lipoprotein cholesterol (HDL-C) was determined after sodium phosphotungstate/magnesium chloride precipitation of low-density lipoproteins by polyvinyl sulfate. 
polyvinyl sulfate
Serum apolipoproteins were quantified by radial immunodiffusion.



radial immunodiffusion