Unraveling DNA Organization with Single-Molecule Force Spectroscopy Using Magnetic Tweezers.Genomes carry the genetic blueprint of all living organisms.  Magnetic tweezers in particular have the unique ability to complement such force spectroscopy with the control over the linking number of the DNA molecule, which plays an important role when DNA organizing proteins form or release wraps, loops, and bends in DNA.  Here, we describe all the necessary steps to prepare DNA substrates for magnetic tweezers experiments, assemble flow cells, tether DNA to magnetics bead inside flow cell, and manipulate and record the extension of such DNA tethers. 
Magnetic tweezers
Unraveling DNA Organization with Single-Molecule Force Spectroscopy Using Magnetic Tweezers.Genomes carry the genetic blueprint of all living organisms.  The study of the structure and dynamics of the proteins that organize the genome has benefited tremendously from the development of single-molecule force spectroscopy techniques that allow for real-time, nanometer accuracy measurements of the compaction of DNA and manipulation with pico-Newton scale forces. 
single-molecule force spectroscopy