Development of a Method for Determination of Buckwheat Allergens Using Liquid Chromatography with Tandem Mass Spectrometry.An analysis technique using LC with tandem MS (MS/MS) has been developed for the determination of buckwheat proteins, including major allergens. 
LC with tandem MS
Peptide spectra were obtained using full-scan LC-MS/MS analysis, and peptide sequences were determined through a protein search.  These findings indicate that LC-MS/MS analysis may be applied to the detection of buckwheat food allergens.
LC-MS/MS
Peptide spectra were obtained using full-scan LC-MS/MS analysis, and peptide sequences were determined through a protein search.  These findings indicate that LC-MS/MS analysis may be applied to the detection of buckwheat food allergens.
LC-MS/MS