Prior to assay EXO1 on different DNA flap structures, we determined factors essential for the thermodynamic stability of EXO1.  Furthermore, we report novel mechanistic insights into the processing of flap structures, showing that EXO1 preferentially cleaves one nucleotide inwards in a double stranded region of a forked and nicked DNA flap substrates, suggesting a possible role of EXO1 in strand displacement.
THERM