Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9.Recent advances in gene editing techniques have not been exploited in farmed fishes.  We established a gene targeting technique, using the CRISPR/Cas9 system in Labeo rohita, a farmed carp (known as rohu).  We demonstrated that donor DNA was integrated via homologous recombination (HR) at the site of targeted double-stranded nicks created by CRISPR/Cas9 nuclease.  Altogether, this is the first evidence that the CRISPR/Cas9 system is a highly efficient tool for targeted gene disruption via HR in teleosts for generating model large-bodied farmed fishes.
CRISPR/Cas9
Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9.Recent advances in gene editing techniques have not been exploited in farmed fishes.  We established a gene targeting technique, using the CRISPR/Cas9 system in Labeo rohita, a farmed carp (known as rohu).  We demonstrated that donor DNA was integrated via homologous recombination (HR) at the site of targeted double-stranded nicks created by CRISPR/Cas9 nuclease.  Altogether, this is the first evidence that the CRISPR/Cas9 system is a highly efficient tool for targeted gene disruption via HR in teleosts for generating model large-bodied farmed fishes.
CRISPR/Cas9
Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9.Recent advances in gene editing techniques have not been exploited in farmed fishes.  We established a gene targeting technique, using the CRISPR/Cas9 system in Labeo rohita, a farmed carp (known as rohu).  We demonstrated that donor DNA was integrated via homologous recombination (HR) at the site of targeted double-stranded nicks created by CRISPR/Cas9 nuclease.  Altogether, this is the first evidence that the CRISPR/Cas9 system is a highly efficient tool for targeted gene disruption via HR in teleosts for generating model large-bodied farmed fishes.
CRISPR/Cas9