By applying an 80 % nucleotide cutoff value and the RotaC(2.0) automated genotyping tool, the Vp4 genotype of the new isolate was identified as P[7]. 
RotaC(2.0)
Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 10(6.125) TCID50/ml. 
MA-104 cells
Rotavirus-specific cytopathic effects were observed after 12 blind passages on MA-104 cells, yielding a virus titer of 10(6.125) TCID50/ml. 
TCID50/ml