Recombination-assisted megaprimer (RAM) cloning.No molecular cloning technique is considered universally reliable, and many suffer from being too laborious, complex, or expensive.  This new method, recombination-assisted megaprimer (RAM) cloning, significantly extends the application of restriction-free cloning, and allows efficient vector construction with much less time and effort when restriction-free cloning fails to provide satisfactory results. 
RAM
The following modifications were made to the protocol:•Limited number of PCR cycles for both megaprimer synthesis and the cloning reaction to reduce error propagation.
PCR
Recombination-assisted megaprimer (RAM) cloning.No molecular cloning technique is considered universally reliable, and many suffer from being too laborious, complex, or expensive.  This new method, recombination-assisted megaprimer (RAM) cloning, significantly extends the application of restriction-free cloning, and allows efficient vector construction with much less time and effort when restriction-free cloning fails to provide satisfactory results. 
RAM
The following modifications were made to the protocol:•Limited number of PCR cycles for both megaprimer synthesis and the cloning reaction to reduce error propagation.
PCR
Recombination-assisted megaprimer (RAM) cloning.No molecular cloning technique is considered universally reliable, and many suffer from being too laborious, complex, or expensive.  This new method, recombination-assisted megaprimer (RAM) cloning, significantly extends the application of restriction-free cloning, and allows efficient vector construction with much less time and effort when restriction-free cloning fails to provide satisfactory results. 
RAM