We took advantage of reverse transcription followed by droplet digital PCR (RT ddPCR), a highly sensitive and precise technology for determination of template concentrations without the use of a standard curve.  As it has been reported previously, we observed a lower coefficient of variation among technical replicates for ddPCR compared to qPCR.  Results furthermore established RT ddPCR as a sensitive and quantitative method for detecting DI gene segments within influenza A virus stocks.
ddPCR
We took advantage of reverse transcription followed by droplet digital PCR (RT ddPCR), a highly sensitive and precise technology for determination of template concentrations without the use of a standard curve.  Results furthermore established RT ddPCR as a sensitive and quantitative method for detecting DI gene segments within influenza A virus stocks.
RT ddPCR
Results were compared to those generated using standard RT qPCR.  As it has been reported previously, we observed a lower coefficient of variation among technical replicates for ddPCR compared to qPCR. 
qPCR