In the present study, we used Isobaric Tag for Relative and Absolute Quantification (iTRAQ) analysis to identify 1691 proteins, which can be used to determine the role of NO in regulating the molecular changes of proteome in cucumber leaves exposed to Cd stress.  Taken together, this iTRAQ analysis provides more comprehensive insights into the physiological and molecular mechanisms of NO against Cd toxicity in cucumber plants.
iTRAQ
In the present study, we used Isobaric Tag for Relative and Absolute Quantification (iTRAQ) analysis to identify 1691 proteins, which can be used to determine the role of NO in regulating the molecular changes of proteome in cucumber leaves exposed to Cd stress. 
Isobaric Tag for Relative and Absolute Quantification