Mapping antiretroviral drugs in tissue by IR-MALDESI MSI coupled to the Q Exactive and comparison with LC-MS/MS SRM assay.This work describes the coupling of the IR-MALDESI imaging source with the Q Exactive mass spectrometer.  In addition, a targeted MS(2) imaging experiment was also conducted to demonstrate the capabilities of the Q Exactive and to highlight the added selectivity that can be obtained with SRM or MRM imaging experiments.
Q Exactive
Mapping antiretroviral drugs in tissue by IR-MALDESI MSI coupled to the Q Exactive and comparison with LC-MS/MS SRM assay.This work describes the coupling of the IR-MALDESI imaging source with the Q Exactive mass spectrometer.  IR-MALDESI MSI was used to elucidate the spatial distribution of several HIV drugs in cervical tissues that had been incubated in either a low or high concentration.  Serial sections of those analyzed by IR-MALDESI MSI were homogenized and analyzed by LC-MS/MS to quantify the amount of each drug present in the tissue.  This correlation between these two techniques serves as a prerequisite to quantitative IR-MALDESI MSI. 
IR-MALDESI MSI
Mapping antiretroviral drugs in tissue by IR-MALDESI MSI coupled to the Q Exactive and comparison with LC-MS/MS SRM assay.This work describes the coupling of the IR-MALDESI imaging source with the Q Exactive mass spectrometer. 
LC-MS/MS SRM assay