Pseudomonas aeruginosa Inhibits the Growth of Scedosporium and Lomentospora In Vitro.In vitro bacterial-fungal interaction studies in cystic fibrosis (CF) have mainly focused on interactions between bacteria and Candida.  Here we investigated the effect of Pseudomonas aeruginosa on the growth of Scedosporium/Lomentospora spp.  Following incubation with XTT, inhibition of fungal growth was determined as the ratio of absorbance in liquid culture with Pseudomonas to that in control fungal cultures. 
Pseudomonas
Pseudomonas aeruginosa Inhibits the Growth of Scedosporium and Lomentospora In Vitro.In vitro bacterial-fungal interaction studies in cystic fibrosis (CF) have mainly focused on interactions between bacteria and Candida.  Here we investigated the effect of Pseudomonas aeruginosa on the growth of Scedosporium/Lomentospora spp.  Standard suspensions of P. aeruginosa (16 non-mucoid and nine mucoid isolates) were dropped onto paper disks, placed on lawns of Lomentospora prolificans (formerly Scedosporium prolificans) strain WM 14.140 or Scedosporium aurantiacum strain WM 11.78 on solid agar.  As P. aeruginosa undergoes phenotype transitions from non-mucoid to the mucoid form with progression of CF lung disease, this balance may influence the appearance of Scedosporium fungi in the airways.
Scedosporium
Pseudomonas aeruginosa Inhibits the Growth of Scedosporium and Lomentospora In Vitro.In vitro bacterial-fungal interaction studies in cystic fibrosis (CF) have mainly focused on interactions between bacteria and Candida.  Here we investigated the effect of Pseudomonas aeruginosa on the growth of Scedosporium/Lomentospora spp.  Standard suspensions of P. aeruginosa (16 non-mucoid and nine mucoid isolates) were dropped onto paper disks, placed on lawns of Lomentospora prolificans (formerly Scedosporium prolificans) strain WM 14.140 or Scedosporium aurantiacum strain WM 11.78 on solid agar. 
Lomentospora
Inhibition of both L. prolificans and S. aurantiacum by these strains was confirmed using the XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide) reduction assay.  Following incubation with XTT, inhibition of fungal growth was determined as the ratio of absorbance in liquid culture with Pseudomonas to that in control fungal cultures. 
XTT