NanoString nCounter technology and bioinformatics analyzed miRNA expression levels and their targets during mouse corneal epithelial wound healing. 
NanoString nCounter technology
NanoString nCounter technology and bioinformatics analyzed miRNA expression levels and their targets during mouse corneal epithelial wound healing. 
bioinformatics
Real-time RT-PCR was performed to detect miR-204 expression in mouse corneal epithelium. 
Real-time RT-PCR
Human corneal epithelial cells (HCECs) were transfected with miR-204 using transfection reagent. 
transfection reagent
MTS (3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]-2H-tetrazolium, inner salt) and a scratch wound-healing assay evaluated the effects of miR-204 expression on HCEC proliferation and migration, respectively. 
scratch wound-healing assay
Cell cycle analysis was performed by flow cytometry. 
flow cytometry
Expression of sirtuin 1 (SIRT1) was determined by Western blot analysis.



Western blot analysis