To investigate the antifibrotic effect of Pyk2-related non-kinase (PRNK) and explore the possibility of using adenovirus carrying PRNK gene for targeted inhibition of Pyk2 to treat myocardial fibrosis.


 Adenovirus carrying PRNK gene was constructed and the angiotention II (Ang II)-induced rat cardiac fibroblasts (CFs) were transfected with the adenovirus.  After the preparation of rat model of abdominal aortic stenosis, the rats were infected by the adenovirus expressing PRNK gene.  Adenoviral vector carrying PRNK gene was successfully constructed; rat CFs were effectively transfected by the adenovirus that expressed PRNK gene stably.  The adenoviruses were injected into rats with myocardial interstitial fibrosis via the tail vein. 
adenovirus
To investigate the antifibrotic effect of Pyk2-related non-kinase (PRNK) and explore the possibility of using adenovirus carrying PRNK gene for targeted inhibition of Pyk2 to treat myocardial fibrosis.


 Adenovirus carrying PRNK gene was constructed and the angiotention II (Ang II)-induced rat cardiac fibroblasts (CFs) were transfected with the adenovirus.  After the preparation of rat model of abdominal aortic stenosis, the rats were infected by the adenovirus expressing PRNK gene.  Adenoviral vector carrying PRNK gene was successfully constructed; rat CFs were effectively transfected by the adenovirus that expressed PRNK gene stably.  The adenoviruses were injected into rats with myocardial interstitial fibrosis via the tail vein. 
adenovirus
To investigate the antifibrotic effect of Pyk2-related non-kinase (PRNK) and explore the possibility of using adenovirus carrying PRNK gene for targeted inhibition of Pyk2 to treat myocardial fibrosis.


 Adenovirus carrying PRNK gene was constructed and the angiotention II (Ang II)-induced rat cardiac fibroblasts (CFs) were transfected with the adenovirus.  After the preparation of rat model of abdominal aortic stenosis, the rats were infected by the adenovirus expressing PRNK gene.  Adenoviral vector carrying PRNK gene was successfully constructed; rat CFs were effectively transfected by the adenovirus that expressed PRNK gene stably.  The adenoviruses were injected into rats with myocardial interstitial fibrosis via the tail vein. 
adenovirus
To investigate the antifibrotic effect of Pyk2-related non-kinase (PRNK) and explore the possibility of using adenovirus carrying PRNK gene for targeted inhibition of Pyk2 to treat myocardial fibrosis.


 Adenovirus carrying PRNK gene was constructed and the angiotention II (Ang II)-induced rat cardiac fibroblasts (CFs) were transfected with the adenovirus.  After the preparation of rat model of abdominal aortic stenosis, the rats were infected by the adenovirus expressing PRNK gene.  Adenoviral vector carrying PRNK gene was successfully constructed; rat CFs were effectively transfected by the adenovirus that expressed PRNK gene stably.  The adenoviruses were injected into rats with myocardial interstitial fibrosis via the tail vein. 
adenovirus
TUNEL method was used to detect myocardial cell apoptosis, and secretions of type I and IV collagen in myocardial tissues were detected by ELISA; expressions of PRNK and phosphorylated Pyk2 proteins were detected by Western Blot.



ELISA
TUNEL method was used to detect myocardial cell apoptosis, and secretions of type I and IV collagen in myocardial tissues were detected by ELISA; expressions of PRNK and phosphorylated Pyk2 proteins were detected by Western Blot.



Western Blot