Different statistical analyses were performed using SPSS v 22.0. 
SPSS
Sequence based variant effect was predicted using Variant Effect Predictor. 
Variant Effect Predictor
To evaluate whether variation in KIR3DL1 and HLA interaction changes the binding affinity structure based effect prediction was carried out using MutaBind and BindProf software.



MutaBind
To evaluate whether variation in KIR3DL1 and HLA interaction changes the binding affinity structure based effect prediction was carried out using MutaBind and BindProf software.



BindProf