Ribotrap analysis of proteins associated with FHL3 3'untranslated region in glioma cells.OBJECTIVE

Ribotrap
Western blot was adopted to detect the regulatory effect of poly(C)-binding protein 2 (PCBP2) on FHL3. 
Western blot
Biotin pull-down and sliver staining were employed to screen and verify the candidate binding proteins of FHL3 3'UTR. 
Biotin pull-down
Then liquid chromatography-tandem mass spectrometry (LC-MS/MS) and molecule annotation system were used to identify and analyze the candidate binding proteins.  Immuno- precipitation was conducted to study the interaction between PCBP2 and polypyrimidine tract-binding protein 1 (PTBP1), a binding protein identified by LC-MS/MS.



LC-MS/MS
Then liquid chromatography-tandem mass spectrometry (LC-MS/MS) and molecule annotation system were used to identify and analyze the candidate binding proteins. 
Molecule annotation system
Immuno- precipitation was conducted to study the interaction between PCBP2 and polypyrimidine tract-binding protein 1 (PTBP1), a binding protein identified by LC-MS/MS.



Immuno-precipitation