IL23R gene expressions in vitro intervened by different concentrations baicalin were detected using Q-PCR. 
Q-PCR
Expressions of signal transducer and activator of transcription 4 (STAT4) , STAT6, phosphorylated-STAT4 (p-STAT4), and p-STAT6 were detected using Western blot. 
Western blot
Serum levels of IFN-γ, IL-4, IL-6, and IL-10 were measured by ELISA. 
ELISA