The relative amounts of MUC5AC and MUC16 were quantified with densitometry using software and compared for statistically significant differences between tear collection methods using the Kruskal-Wallis test in SPSS 22 and GraphPad Prism 7.02. 
SPSS
The relative amounts of MUC5AC and MUC16 were quantified with densitometry using software and compared for statistically significant differences between tear collection methods using the Kruskal-Wallis test in SPSS 22 and GraphPad Prism 7.02. 
GraphPad Prism