The present study obtained a germ cell-specific marker dead end (dnd) in olive flounder (Paralichthys olivaceus) named Podnd.  The tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected.  SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells.  The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable.  The Podnd transcripts localization and migration were similar to zebrafish. 
Podnd
SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. 
SISH