Liquid chromatography/electrospray ionisation tandem mass spectrometric study of sitagliptin and its stressed degradation products.A sensitive UPLC positive ion electrospray tandem mass spectrometry method was developed and validated for identification of degradation products of sitagliptin formed during stress study. 
UPLC
The separation of sitagliptin and its degradation products was achieved on a Acquity BEH C-18 column (50×2.1 mm, 1.7 µm) using a gradient programme. 
Acquity BEH C-18 column
Suitability of this method for the quantitative determination of the drugs was proved by validation in accordance with International Conference on Harmonization (ICH) guidelines and can be used for routine analysis of sitagliptin formulations in quality control.
International Conference on Harmonization (ICH) guidelines