time-ChIP: A Method to Determine Long-Term Locus-Specific Nucleosome Inheritance.Understanding chromatin dynamics is essential to define the contribution of chromatin to heritable gene silencing and the long-term maintenance of gene expression.  Here we present a detailed protocol for time-ChIP, a novel method to measure histone turnover at high resolution across long timescales.  time-ChIP can define regions of high and low histone turnover and identify the location of pools of long lived histones.
time-ChIP
This method is based on the SNAP-tag, a self-labeling enzyme that can be pulse labeled with small molecules in cells.  Upon pulse biotinylation of a cohort of SNAP-tagged histones we can determine their abundance and fate across a chase period using a biotin-specific chromatin pulldown followed by DNA sequencing or quantitative PCR. 
SNAP-tag
Upon pulse biotinylation of a cohort of SNAP-tagged histones we can determine their abundance and fate across a chase period using a biotin-specific chromatin pulldown followed by DNA sequencing or quantitative PCR. 
biotin-specific chromatin pulldown