First, half-maximal inhibitory concentrations (IC50) of cisplatin, creatine and pioglitazone were calculated by RTCA system.  Afterwards creatine and pioglitazone was administered with serial dilutions under RTCA system. 
RTCA system
Real-time cell analyzer system (RTCA) was used for real-time and time-dependent analysis of the cellular response of HK-2 cells following incubation with cisplatin and combination with creatine or pioglitazone hydrochloride. 
Real-time cell analyzer system