Agilent microarray was used to detect changes in gene expression between CD8+ T cells cultured with and without the SKOV3 ovarian adenocarcinoma cell line. 
Agilent microarray
QRT-PCR was performed to determine glycolysis gene expression in CD8+ T cells from a transwell culturing system and OC patients. 
QRT-PCR
We also detected protein levels of glycolysis-related genes using Western blot analysis.



Western blot analysis