Here, we present a Multi-Animal Tracker (MAT) that provides a user-friendly, end-to-end solution for imaging, tracking, and analyzing complex behaviors of multiple animals simultaneously.  Focusing on C. elegans worms, we demonstrate the vast advantages of using this MAT in studying complex behaviors.  Beginning with chemotaxis, we show that approximately 100 animals can be tracked simultaneously, providing rich behavioral data.  Next, we show that worms can integrate environmental information and that directional changes mediate the enhanced chemotaxis towards richer environments. 
MAT
A multi-animal tracker for studying complex behaviors.BACKGROUND
 Here, we present a Multi-Animal Tracker (MAT) that provides a user-friendly, end-to-end solution for imaging, tracking, and analyzing complex behaviors of multiple animals simultaneously. 
Multi-Animal Tracker