In this study, we used small RNA sequencing and bioinformatic methods to identify novel microRNA regulating gene expression during hibernation in thirteen-lined ground squirrels, Ictidomys tridecemlineatus. 
small RNA sequencing
In this study, we used small RNA sequencing and bioinformatic methods to identify novel microRNA regulating gene expression during hibernation in thirteen-lined ground squirrels, Ictidomys tridecemlineatus. 
bioinformatic methods
A group of 17 novel microRNA was identified, and their relative expression was quantitated using real-time quantitative polymerase chain reaction in liver, skeletal muscle, and heart tissues over four experimental conditions that represent the torpor-arousal cycle. 
real-time quantitative polymerase chain reaction