We had identified differentially expressed genes (DEGs) in AS based on our previous RNA-sequencing results.  Based on the shared DEGs of AS in both integrated analysis and our previous RNA-sequencing results, we constructed the protein and protein interaction (PPI) network. 
RNA-sequencing
In this present study, we identified 1328 DEGs between AS and normal control by using integrated analysis of two datasets derived from the Gene Expression Omnibus (GEO) database. 
Gene Expression Omnibus (GEO)
Validation of gene expression by qRT-PCR were performed and the results were consistent with our integrated analysis generally. 
qRT-PCR