Strains were identified and re-identified using established methods, NEFERMtest 24 (MICROLATEST) biochemical microtest and API (bioMerieux) test systems were used. 
NEFERMtest 24
Strains were identified and re-identified using established methods, NEFERMtest 24 (MICROLATEST) biochemical microtest and API (bioMerieux) test systems were used. 
MICROLATEST
Strains were identified and re-identified using established methods, NEFERMtest 24 (MICROLATEST) biochemical microtest and API (bioMerieux) test systems were used. 
API (bioMerieux)
For all carbapenem-resistant strains a phenotype screening for MBL was performed using the double-disks method with EDTA. 
double-disks method with EDTA
In order to identify VIM-type and IMP-type MBL genes a real-time multiplex polymerase chain reaction was used. 
real-time multiplex polymerase chain reaction