Genotyping of exons 1 to 20 in Duchenne muscular dystrophy by universal multiplex PCR and short-end capillary electrophoresis.One rapid CE method was established to diagnose Duchenne muscular dystrophy (DMD).  In this study, the universal multiplex PCR was used to enhance the fluorescently labeling efficiency, which was performed only by one universal fluorescent primer.  After PCR, a short-end injection CE (short-end CE) speeded up the genotyping of the DMD gene. 
PCR
Genotyping of exons 1 to 20 in Duchenne muscular dystrophy by universal multiplex PCR and short-end capillary electrophoresis.One rapid CE method was established to diagnose Duchenne muscular dystrophy (DMD).  DMD is a severe recessive inherited disorder frequently caused by gene deletions.  Among them, exons 1-20 account for nearly 30% of occurrences.  In this study, the universal multiplex PCR was used to enhance the fluorescently labeling efficiency, which was performed only by one universal fluorescent primer.  After PCR, a short-end injection CE (short-end CE) speeded up the genotyping of the DMD gene.  The CE conditions contained a polymer solution of 1.5% hydroxylethylcellulose in 1× TBE buffer at 6 kV for separation. 
CE