The effects of AKBA on cell viability and its cytotoxicity were determined using CCK8 and LDH kits respectively. 
CCK8
The effects of AKBA on cell viability and its cytotoxicity were determined using CCK8 and LDH kits respectively. 
LDH kits
The EdU-DNA synthesis assay was used to evaluate inhibition of cell proliferation by AKBA. 
EdU-DNA synthesis assay
The role of AKBA in glioblastoma cell functions such as migration/invasion, and colony formation was evaluated using transwell chambers and soft agar, respectively. 
transwell chambers
The role of AKBA in glioblastoma cell functions such as migration/invasion, and colony formation was evaluated using transwell chambers and soft agar, respectively. 
soft agar
Flow cytometry and western blotting were used to detect AKBA-induced apoptosis. 
flow cytometry
Flow cytometry and western blotting were used to detect AKBA-induced apoptosis.  Potential mechanisms of AKBA action were explored by RNA sequencing and the identified hub genes were validated by real-time quantitative PCR and western blotting.  These findings were validated by qRT-PCR and western blotting. 
western blotting
Potential mechanisms of AKBA action were explored by RNA sequencing and the identified hub genes were validated by real-time quantitative PCR and western blotting. 
RNA sequencing
Potential mechanisms of AKBA action were explored by RNA sequencing and the identified hub genes were validated by real-time quantitative PCR and western blotting. 
real-time quantitative PCR