CRISPR-Cas Genome Surgery in Ophthalmology.Genetic disease affecting vision can significantly impact patient quality of life.  Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated systems (Cas) represent powerful tools for studying diseases through the creation of model organisms generated by targeted modification and by the correction of disease mutations for therapeutic purposes.  CRISPR-Cas systems have been applied successfully to the visual sciences and study of ophthalmic disease - from the modification of zebrafish and mammalian models of eye development and disease, to the correction of pathogenic mutations in patient-derived stem cells.  Recent advances in CRISPR-Cas delivery and optimization boast improved functionality that continues to enhance genome-engineering applications in the eye.  This review provides a synopsis of the recent implementations of CRISPR-Cas tools in the field of ophthalmology.
CRISPR
CRISPR-Cas Genome Surgery in Ophthalmology.Genetic disease affecting vision can significantly impact patient quality of life.  CRISPR-Cas systems have been applied successfully to the visual sciences and study of ophthalmic disease - from the modification of zebrafish and mammalian models of eye development and disease, to the correction of pathogenic mutations in patient-derived stem cells.  Recent advances in CRISPR-Cas delivery and optimization boast improved functionality that continues to enhance genome-engineering applications in the eye.  This review provides a synopsis of the recent implementations of CRISPR-Cas tools in the field of ophthalmology.
CRISPR-Cas