Three-dimensional (3D) structure of the local Eg14-3-3 Ag was modeled by EasyModeller software. 
EasyModeller
DNA samples were extracted from the protoscoleces of the infected sheep's liver, and then subjected to the polymerase chain reaction (PCR) with 14-3-3 specific forward and reverse primers. 
polymerase chain reaction (PCR)
The final designed construct was analyzed based on different physicochemical properties, which was then codon optimized for high-level expression in Escherichia coli k12. 
Escherichia coli k12