They were screened for the presence of Babesia/Theileria and Ehrlichia/Anaplasma genomic DNA using PCR and Reverse Line Blot (RLB) assays.  Positive B. rossi specimens were tested for the presence of the BrEMA1gene using an RT-PCR.  Results of RT-PCR screening for the BrEMA1 gene were negative. 
PCR
They were screened for the presence of Babesia/Theileria and Ehrlichia/Anaplasma genomic DNA using PCR and Reverse Line Blot (RLB) assays. 
Reverse Line Blot (RLB)
Positive B. rossi specimens were tested for the presence of the BrEMA1gene using an RT-PCR.  Results of RT-PCR screening for the BrEMA1 gene were negative. 
RT-PCR