Wheat germ systems for cell-free protein expression.Cell-free protein expression plays an important role in biochemical research. 
Wheat germ systems
Wheat germ systems for cell-free protein expression.Cell-free protein expression plays an important role in biochemical research.  However, only recent developments led to new methods to rapidly synthesize preparative amounts of protein that make cell-free protein expression an attractive alternative to cell-based methods.  In particular the wheat germ system provides the highest translation efficiency among eukaryotic cell-free protein expression approaches and has a very high success rate for the expression of soluble proteins of good quality.  Here I describe wheat germ cell-free protein expression systems and give examples how they have been used in genome-wide expression studies, preparation of labeled proteins for structural genomics and protein mass spectroscopy, automated protein synthesis, and screening of enzymatic activities. 
cell-free protein expression