Virus-induced gene silencing (VIGS) of tomato fruits have been used to down-regulate SlCHS expression during ripening and analyze the effects at the epidermal and cuticle level. 
Virus-induced gene silencing
CHS silencing suggests a negative cross-talk between wax and flavonoid pathways in tomato fruit cuticle.Tomato fruits (Solanum lycopersicum L.) accumulate flavonoids in their cuticle and epidermal cells during ripening.  These flavonoids come from de novo biosynthesis due to a significant increase in chalcone synthase (CHS) activity during ripening.  Virus-induced gene silencing (VIGS) of tomato fruits have been used to down-regulate SlCHS expression during ripening and analyze the effects at the epidermal and cuticle level.  These indicate a role for either flavonoids or CHS in the alteration of the expression levels of some genes involved in cuticle biosynthesis. 
CHS