Needleless emulsion electrospinning for the regulated delivery of susceptible proteins.In the present work, we developed a novel needleless emulsion electrospinning technique that improves the production rate of the core/shell production process.  Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase [i.e. 5-(4,6-dichlorotriazinyl) aminofluorescein -PF-68, horseradish peroxidase, Tetramethylrhodamine-dextran, insulin growth factor-I, transforming growth factor-β and basic fibroblast growth factor]. 
Needleless emulsion electrospinning
The nanofibres are based on poly-ε-caprolactone (PCL) as a continuous phase combined with a droplet phase based on Pluronic F-68 (PF-68).  The PCL-PF-68 nanofibres show a time-regulated release of active molecules.  Needleless emulsion electrospinning was used to encapsulate a diverse set of compounds to the core phase [i.e. 5-(4,6-dichlorotriazinyl) aminofluorescein -PF-68, horseradish peroxidase, Tetramethylrhodamine-dextran, insulin growth factor-I, transforming growth factor-β and basic fibroblast growth factor].  In addition, the PF-68 facilitates the preservation of the bioactivity of delivered proteins. 
PF-68
The PCL-PF-68 nanofibres show a time-regulated release of active molecules. 
PCL-PF-68 nanofibres