In this work, physical and chemical coupled modification method, that is the combination of nanoimprinting technique and graft polymerization, was adopted to modify FA on nanopatterned polydimethylsiloxane (PDMS) surface for possible application in micro-nanofluidic cytology. 
nanoimprinting technique
In this work, physical and chemical coupled modification method, that is the combination of nanoimprinting technique and graft polymerization, was adopted to modify FA on nanopatterned polydimethylsiloxane (PDMS) surface for possible application in micro-nanofluidic cytology. 
graft polymerization
The surface property of differently treated PDMS was characterized by FTIR, AFM and contact angle measurement. 
FTIR
The surface property of differently treated PDMS was characterized by FTIR, AFM and contact angle measurement. 
AFM
The surface property of differently treated PDMS was characterized by FTIR, AFM and contact angle measurement. 
contact angle measurement
AO/PI double staining, cell counting and MTT method were performed to examine the potential influence of FA modified nanopatterned PDMS on human cervical carcinoma (HeLa) cell behavior. 
AO/PI double staining
AO/PI double staining, cell counting and MTT method were performed to examine the potential influence of FA modified nanopatterned PDMS on human cervical carcinoma (HeLa) cell behavior. 
cell counting
AO/PI double staining, cell counting and MTT method were performed to examine the potential influence of FA modified nanopatterned PDMS on human cervical carcinoma (HeLa) cell behavior. 
MTT method