Candida albicans DBF4 gene inducibly duplicated by the mini-Ura-blaster is involved in hypha-suppression.The opportunistic human fungal pathogen Candida albicans is a natural diploid that does not have a complete sexual cycle.  We made a C. albicans strain, with one DBF4 allele deleted by the mini-Ura-blaster and the other controlled by a repressible promoter.  We conclude that the CaDBF4 gene is prone to being duplicated by the mini-Ura-blaster and that it suppresses hyphal growth in C. albicans.
mini-Ura-blaster
Candida albicans DBF4 gene inducibly duplicated by the mini-Ura-blaster is involved in hypha-suppression.The opportunistic human fungal pathogen Candida albicans is a natural diploid that does not have a complete sexual cycle.  We made a C. albicans strain, with one DBF4 allele deleted by the mini-Ura-blaster and the other controlled by a repressible promoter.  We conclude that the CaDBF4 gene is prone to being duplicated by the mini-Ura-blaster and that it suppresses hyphal growth in C. albicans.
mini-Ura-blaster