We propose a new image restoration method which we call 'Covariance Wiener Filtering' (CWF).  In CWF, the covariance matrix of the projection images is used within the classical Wiener filtering framework for solving the image restoration deconvolution problem.  We demonstrate the efficacy of CWF by applying it to restore both simulated and experimental cryo-EM images.  Results with experimental datasets demonstrate that CWF provides a good way to evaluate the particle images and to see what the dataset contains even without 2D classification and averaging.
CWF
We propose a new image restoration method which we call 'Covariance Wiener Filtering' (CWF). 
Covariance Wiener Filtering