Overexpression of the IbOr gene from sweet potato (Ipomea batatas 'Hoang Long') in maize increases total carotenoid and β-carotene contents.Nutritional quality of most maize varieties is very low due to the lack of lysine and tryptophan and extremely low provitamin A carotenoids including β-carotene, α-carotene, and β-cryptoxanthin.  In this study, we report the successful overexpression of the IbOr gene in H145 and H95 inbred maize lines under the control of maize seed-specific promoter globulin 1 (Glo1) for the purpose of improving β-carotene in maize.  The results showed that the total carotenoid and β-carotene content of all analyzed transgenic maize plants were significantly higher than those of wild-type lines.  For H145-IbOr transgenic maize, in the best line (H145-IbOr.10), the total carotenoid and β-carotene contents were increased up to 10.36- and 15.11-fold, respectively, compared to the wild type (H145-WT).  In the case of H95-IbOr transgenic plants, 5.58-fold increase in total carotenoid and 7.63-fold increase in β-carotene were achieved in the H95-IbOr.6 line compared to nontransgenic plants (H95-WT).  In all the transgenic plants derived from the wild-type maize line with less carotenoid content (H145-WT), the content of both total carotenoid and β-carotene was higher than in transgenic plants derived from the wild-type maize line having more carotenoid content (H95-WT).  Our research is the first in successful overexpression of IbOr gene in maize.
C