Frokaryotic ex pression vector pET30a-P450 was constructed and the prokaryotic expression conditions were optimized.  Consequently P450 gene of E. senticosus could be expressed successfully by prokaryotic expression vector pET30a-P450. 
pET30a
The recombinant accumulated the highest amount of recombinant protein af ter IPTG (1 mmol x L(-1)) at 27-37 degrees C for 24 h.  Induction temperature, IPTG concentration, medium type and amount of induction time could all influence the expression of target protein, but the impact strength was different.
IPTG
SDS-PAGE analysis showed that the recombinant protein was about 53 kDa. 
SDS-PAGE