The PCR-RFLP results showed only one restriction pattern for each enzyme.  The classical methods used in the mycological laboratories are time-consuming, the PCR-RFLP analysis of the ITS is a reliable tool for the identification of M. canis strains. 
PCR
The PCR-RFLP results showed only one restriction pattern for each enzyme.  The classical methods used in the mycological laboratories are time-consuming, the PCR-RFLP analysis of the ITS is a reliable tool for the identification of M. canis strains. 
PCR-RFLP
Molecular analysis was performed by amplification of the ITS region of rDNA, the amplified region was subjected to enzymatic digestion and sequenced to evaluate phylogenetic relationships.  The classical methods used in the mycological laboratories are time-consuming, the PCR-RFLP analysis of the ITS is a reliable tool for the identification of M. canis strains. 
ITS
Molecular analysis was performed by amplification of the ITS region of rDNA, the amplified region was subjected to enzymatic digestion and sequenced to evaluate phylogenetic relationships. 
rDNA