By taking advantage of the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system and the turquoise killifish genome, this platform enables the generation of knockout alleles via nonhomologous end joining (NHEJ) and knock-in alleles via homology-directed repair (HDR). 
CRISPR/Cas9
We also provide strategies for Tol2-based transgenesis and large-scale husbandry conditions that are critical for success. 
Tol2-based transgenesis