Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast.CRISPR-Cas9 loss of function (LOF) and base editing screens are powerful tools in genetics and genomics. 
CRISPR-Cas9
Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast.CRISPR-Cas9 loss of function (LOF) and base editing screens are powerful tools in genetics and genomics.  We developed a double selection strategy based on co-selection that increases LOF mutation rates using the Target-AID base editor.  We constructed the pDYSCKO vector, which is amenable to high throughput double selection experiments, and show that the improvement in Target-AID efficiency generalizes across loci. 
Target-AID
Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast.CRISPR-Cas9 loss of function (LOF) and base editing screens are powerful tools in genetics and genomics.  We show that double selection can also improve Cas9 mediated LOF rates, but that this multiplex genome editing causes programmable chromosomal translocations at high frequency. 
Cas9