Expression, purification, and characterization of bovine chymosin enzyme using an inducible pTOLT system.In recent years, various studies in the field of industrial enzymes of biotechnology have gained importance due to increasing development in enzyme technology.  In this study, the nucleic acid sequence encoding the optimized chymosin enzyme was used and cloned by Not I and Mlu I restriction enzymes into pTOLT vector system. 
pTOLT
In this study, the nucleic acid sequence encoding the optimized chymosin enzyme was used and cloned by Not I and Mlu I restriction enzymes into pTOLT vector system. 
Not I
In this study, the nucleic acid sequence encoding the optimized chymosin enzyme was used and cloned by Not I and Mlu I restriction enzymes into pTOLT vector system. 
Mlu I