Studying SIRT6 regulation using H3K56 based substrate and small molecules.SIRT6 is a modulator of chromatin structure having an important role in healthy ageing, and there is a crucial need to find specific modulators for it.  Therefore, the activity of SIRT6 should be studied using a variety of methods.  We examined the capability of SIRT6 to deacetylate a set of five fluorogenic substrates based on p53 and histone H3 sequences.  Nicotinamide is a known inhibitor for sirtuins, and it was found to be less potent inhibitor for SIRT6 than it is for SIRT1.  EX-527, quercetin and three pseudopeptidic compounds were found to be the most potent SIRT6 inhibitors, exhibiting over 50% deacetylation inhibition.  These findings describe the first modulators of SIRT6 activity at the physiologically important H3K56 deacetylation site.
SIRT6
Studying SIRT6 regulation using H3K56 based substrate and small molecules.SIRT6 is a modulator of chromatin structure having an important role in healthy ageing, and there is a crucial need to find specific modulators for it.  In addition, we studied 15 other small molecule sirtuin modulators using the H3K56 based substrate. 
H3K56 based substrate
Studying SIRT6 regulation using H3K56 based substrate and small molecules.SIRT6 is a modulator of chromatin structure having an important role in healthy ageing, and there is a crucial need to find specific modulators for it.  In addition, we studied 15 other small molecule sirtuin modulators using the H3K56 based substrate. 
small molecules
EX-527, quercetin and three pseudopeptidic compounds were found to be the most potent SIRT6 inhibitors, exhibiting over 50% deacetylation inhibition. 
EX-527
EX-527, quercetin and three pseudopeptidic compounds were found to be the most potent SIRT6 inhibitors, exhibiting over 50% deacetylation inhibition. 
quercetin
EX-527, quercetin and three pseudopeptidic compounds were found to be the most potent SIRT6 inhibitors, exhibiting over 50% deacetylation inhibition. 
pseudopeptidic compounds