Based on the single factor experiments, with encapsulation efficiency (EE) Size of TFDMCPL and polymey disperse index (PDI) as dependent variable, central composite design was adopted to optimize preparation technology by taking content of phospholipid and content of cholesterol as independent variables, fitting of various mathematical equations were performed using a statisitical software of Design-Expert 8.0.6. 
Design-Expert 8.0.6