Multi-site binding of epigallocatechin gallate to human serum albumin measured by NMR and isothermal titration calorimetry.The affinity of epigallocatechin gallate (EGCG) for human serum albumin (HSA) was measured in physiological conditions using NMR and isothermal titration calorimetry (ITC).  NMR estimated the Ka (self-dissociation constant) of EGCG as 50 mM.  NMR showed two binding events: strong (n1=1.8 ± 0.2; Kd1 =19 ± 12 μM) and weak (n2∼20; Kd2 =40 ± 20 mM). 
NMR
Multi-site binding of epigallocatechin gallate to human serum albumin measured by NMR and isothermal titration calorimetry.The affinity of epigallocatechin gallate (EGCG) for human serum albumin (HSA) was measured in physiological conditions using NMR and isothermal titration calorimetry (ITC).  ITC also showed two binding events: strong (n1=2.5 ± 0.03; Kd1 =21.6 ± 4.0 μM) and weak (n2=9 ± 1; Kd2 =22 ± 4 mM). 
ITC