In the current study, vitamin D3 1α-Hydroxylase ["1α(OH)ase"] knockout mice were generated to mimic vitamin D deficiency in vivo. 
1α(OH)ase
Significantly, such effects in the knockout mice were largely attenuated by supplement with anti-oxidant n-acetylcysteine (NAC).  Remarkably, insulin resistance and metabolic abnormalities in the knockout mice were largely alleviated after treatment of NAC.  Therefore, inhibition of oxidative stress by NAC alleviated insulin resistance in vitamin D-deficient mice. 
NAC
Significantly, such effects in the knockout mice were largely attenuated by supplement with anti-oxidant n-acetylcysteine (NAC).  Remarkably, insulin resistance and metabolic abnormalities in the knockout mice were largely alleviated after treatment of NAC.  Therefore, inhibition of oxidative stress by NAC alleviated insulin resistance in vitamin D-deficient mice. 
NAC
Significantly, such effects in the knockout mice were largely attenuated by supplement with anti-oxidant n-acetylcysteine (NAC).  Remarkably, insulin resistance and metabolic abnormalities in the knockout mice were largely alleviated after treatment of NAC.  Therefore, inhibition of oxidative stress by NAC alleviated insulin resistance in vitamin D-deficient mice. 
NAC