Three free software packages, geNorm, NormFinder and BestKeeper were used.  In geNorm analysis, the most stable gene was RPS7 (at a primer concentration 1000 nM) or YWHAZ (500 and 250 nM).  Based on geNorm results at least two genes from among RPS7, RPL30, ACTB or YWHAZ should be chosen for Real Time-PCR result normalization.
geNorm
Three free software packages, geNorm, NormFinder and BestKeeper were used.  According to NormFinder and BestKeeper, ACTB (at all examined primer concentrations) followed by RPS7 and CYC were the most stable genes. 
NormFinder
Three free software packages, geNorm, NormFinder and BestKeeper were used.  According to NormFinder and BestKeeper, ACTB (at all examined primer concentrations) followed by RPS7 and CYC were the most stable genes. 
BestKeeper