Binding mechanism of PicoGreen to DNA characterized by magnetic tweezers and fluorescence spectroscopy.Fluorescent dyes are broadly used in many biotechnological applications to detect and visualize DNA molecules.  In this work we employed magnetic tweezers and fluorescence spectroscopy to investigate the binding of PicoGreen to DNA at room temperature in a concentration-dependent manner.  PicoGreen is an ultrasensitive quinolinium nucleic acid stain exhibiting hardly any background signal from unbound dye molecules.  On the basis of our work, we could identify a concentration-dependent bimodal binding behavior, where, apparently, PicoGreen associates to DNA as an intercalator and minor-groove binder simultaneously.
PicoGreen
Binding mechanism of PicoGreen to DNA characterized by magnetic tweezers and fluorescence spectroscopy.Fluorescent dyes are broadly used in many biotechnological applications to detect and visualize DNA molecules.  In this work we employed magnetic tweezers and fluorescence spectroscopy to investigate the binding of PicoGreen to DNA at room temperature in a concentration-dependent manner.  The results of our magnetic tweezers single-molecule binding study were well supported through analyzing the fluorescent spectra of stained DNA. 
magnetic tweezers
Binding mechanism of PicoGreen to DNA characterized by magnetic tweezers and fluorescence spectroscopy.Fluorescent dyes are broadly used in many biotechnological applications to detect and visualize DNA molecules.  In this work we employed magnetic tweezers and fluorescence spectroscopy to investigate the binding of PicoGreen to DNA at room temperature in a concentration-dependent manner. 
fluorescence spectroscopy