Gut microbiota were determined on days 69, 96-99 and 120 by next generation sequencing of the V3-5 region of the 16S rRNA gene. 
next generation sequencing
The machine learning algorithm Random Forests was used to predict age based on the microbiota.


 Random Forests predicted chronological age based on the microbiota and indicated microbiota immaturity in the LF mice before, but not after, refeeding. 
Random Forests