Genome Sequencing of Steroid Producing Bacteria Using Ion Torrent Technology and a Reference Genome.The Next-Generation Sequencing technology has enormously eased the bacterial genome sequencing and several tens of thousands of genomes have been sequenced during the last 10 years.  Most of the genome projects are published as draft version, however, for certain applications the complete genome sequence is required.In this chapter, we describe the strategy that allowed the complete genome sequencing of Mycobacterium neoaurum NRRL B-3805, an industrial strain exploited for steroid production, using Ion Torrent sequencing reads and the genome of a close strain as the reference. 
Ion Torrent
Genome Sequencing of Steroid Producing Bacteria Using Ion Torrent Technology and a Reference Genome.The Next-Generation Sequencing technology has enormously eased the bacterial genome sequencing and several tens of thousands of genomes have been sequenced during the last 10 years. 
Ion Torrent Technology