Elemental bioimaging and speciation analysis for the investigation of Wilson's disease using μXRF and XANES.A liver biopsy specimen from a Wilson's disease (WD) patient was analyzed by means of micro-X-ray fluorescence (μXRF) spectroscopy to determine the elemental distribution.  First, bench-top μXRF was utilized for a coarse scan of the sample under laboratory conditions.  In order to obtain more detailed elemental information, this ROI was analyzed by synchrotron radiation (SR)-based μXRF with a beam size of 4 μm offering a resolution at the cellular level.  Distribution maps of additional elements to copper and iron like zinc and manganese were obtained due to a higher sensitivity of SR-μXRF. 
μXRF
Elemental bioimaging and speciation analysis for the investigation of Wilson's disease using μXRF and XANES.A liver biopsy specimen from a Wilson's disease (WD) patient was analyzed by means of micro-X-ray fluorescence (μXRF) spectroscopy to determine the elemental distribution.  In addition to this, X-ray absorption near edge structure spectroscopy (XANES) was performed to identify the oxidation states of copper in WD. 
XANES