Purification of RNA Mango Tagged Native RNA-protein Complexes from Cellular Extracts Using TO1-Desthiobiotin Fluorophore Ligand.A native purification strategy using RNA Mango for RNA based purification of RNA-protein complexes is described. 
TO1-Desthiobiotin Fluorophore Ligand
Purification of RNA Mango Tagged Native RNA-protein Complexes from Cellular Extracts Using TO1-Desthiobiotin Fluorophore Ligand.A native purification strategy using RNA Mango for RNA based purification of RNA-protein complexes is described.  The RNA Mango aptamer is first genetically engineered into the RNA of interest.  RNA Mango containing complexes obtained from cleared cellular native extracts are then immobilized onto TO1-Desthiobiotin saturated streptavidin agarose beads.  The beads are washed to remove non-specific complexes and then the RNA Mango containing complexes are eluted by the addition of free biotin to the beads. 
RNA Mango
RNA Mango containing complexes obtained from cleared cellular native extracts are then immobilized onto TO1-Desthiobiotin saturated streptavidin agarose beads. 
streptavidin agarose beads