Herein, we present a novel nanopore-based strategy for ultrasensitive detection of Ramos cells (human Burkitt's lymphoma cells), by combining the enzymatic signal amplification with an aerolysin nanopore sensor. 
aerolysin nanopore sensor
In this assay, an aptamer for Ramos cells was prehybridized with a short complementary DNA.  The presence of target cells causes the target-aptamer complex to unwind to free the complementary DNA, which would subsequently trigger the enzymatic cycling amplification. 
aptamer
In this assay, an aptamer for Ramos cells was prehybridized with a short complementary DNA.  The presence of target cells causes the target-aptamer complex to unwind to free the complementary DNA, which would subsequently trigger the enzymatic cycling amplification.  This process eventually generated a large number of output DNA, which could quantitatively produce characteristic current events when translocated through aerolysin. 
DNA
The presence of target cells causes the target-aptamer complex to unwind to free the complementary DNA, which would subsequently trigger the enzymatic cycling amplification. 
enzymatic cycling amplification