Scanning inverse fluorescence correlation spectroscopy.Scanning Inverse Fluorescence Correlation Spectroscopy (siFCS) is introduced to determine the absolute size of nanodomains on surfaces.  Using a confocal microscope of 270 nm resolution the size of 250 nm domains were estimated by siFCS to 257 ± 12 nm diameter, and 40 nm domains were estimated to 65 ± 26 nm diameter.  Applications of siFCS for sizing of nanodomains and protein clusters in cell membranes are discussed.
siFCS
Scanning inverse fluorescence correlation spectroscopy.Scanning Inverse Fluorescence Correlation Spectroscopy (siFCS) is introduced to determine the absolute size of nanodomains on surfaces.  Using a confocal microscope of 270 nm resolution the size of 250 nm domains were estimated by siFCS to 257 ± 12 nm diameter, and 40 nm domains were estimated to 65 ± 26 nm diameter.  Applications of siFCS for sizing of nanodomains and protein clusters in cell membranes are discussed.
siFCS