Functional characterization was undertaken using A-803467, a selective blocker of Nav1.8, and veratridine as an opener of the voltage gated sodium channels while molecular characterization was done using western blotting and indirect immunofluorescence assays.  In vitro capacitation was induced using heparin, and to study the functional involvement of Nav 1.8 in regulation of capacitation induced hyper sperm motility, A-803467 was used.  Selective blocking of NaV 1.8 by A-803467 at 6 and 8 μM concentration significantly (P < 0.05) decreased the forward progressive sperm motility in a time-dependent manner, while, blocking at higher concentrations (10 and 15 μM) resulted in fast forward motility in spermatozoa after 2 h of incubation and it was observed up to 3 h.  In vitro capacitated spermatozoa treated with A-803467 revealed significant (P < 0.05) reduction in forward progressive motility after 2 h of incubation.  Both A-803467 and veratridine altered the percentage of spermatozoa showing high mitochondrial transmembrane potential in concentration- and time-dependent manner.  High concentrations (10 and 15 μM) of A-803467 and veratridine resulted in bent neck condition in spermatozoa along with significant (P < 0.05) reduction in membrane integrity (HOST negative). 
A-803467
Functional characterization was undertaken using A-803467, a selective blocker of Nav1.8, and veratridine as an opener of the voltage gated sodium channels while molecular characterization was done using western blotting and indirect immunofluorescence assays.  Treatment of sperm cells with veratridine (6, 8, 10, 15, 20 μM) resulted in concentration- and time-dependent increase in forward progressive sperm motility and it persisted up to 4 h.  However, hyperactive motility was induced by veratridine at higher concentrations (10 and 15 μM) after 2 h of incubation.  Both A-803467 and veratridine altered the percentage of spermatozoa showing high mitochondrial transmembrane potential in concentration- and time-dependent manner.  High concentrations (10 and 15 μM) of A-803467 and veratridine resulted in bent neck condition in spermatozoa along with significant (P < 0.05) reduction in membrane integrity (HOST negative). 
veratridine