Bacteria engineered to express the lux gene cassette permit BLI detection of the bacteria and tumor sites concurrently.  This protocol outlines the procedure for studying lux-tagged Escherichia coli and Bifidobacterium breve in mice, demonstrating the spatial and temporal readout from 2D and 3D BLI achievable with whole-body in vivo luminescence imaging.
lux
In Vivo Bioluminescence Imaging of Intratumoral Bacteria.This chapter describes the use of whole-body bioluminescent imaging (BLI) for the study of bacterial trafficking in live mice, with an emphasis on the use of bacteria in therapy of cancer.  Bacteria engineered to express the lux gene cassette permit BLI detection of the bacteria and tumor sites concurrently.  This protocol outlines the procedure for studying lux-tagged Escherichia coli and Bifidobacterium breve in mice, demonstrating the spatial and temporal readout from 2D and 3D BLI achievable with whole-body in vivo luminescence imaging.
BLI
Bacteria engineered to express the lux gene cassette permit BLI detection of the bacteria and tumor sites concurrently. 
lux gene cassette