TR-FRET Assays for Endogenous Huntingtin Protein Level in Mouse Cells.High-throughput measurement of huntingtin (Htt) levels is useful for Huntington's disease research.  In the human cells, high-throughput measurement of Htt levels has been established based on the Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) technology, using the 2B7/MW1 antibody pair.  Unfortunately, application of this assay in the mouse cells has been problematic due to discrepancies between TR-FRET signals and Western-blots, possibly caused by non-specific antibody binding.  Here we report TR-FRET assays that are able to detect endogenous Htt levels of the mouse striatal cell line (STHdh).
TR-FRET
TR-FRET Assays for Endogenous Huntingtin Protein Level in Mouse Cells.High-throughput measurement of huntingtin (Htt) levels is useful for Huntington's disease research.  Here we report TR-FRET assays that are able to detect endogenous Htt levels of the mouse striatal cell line (STHdh).
TR-FRET Assays