Printed fluorescence imaging boxes (PFIboxes) cost approximately 200 US dollars to assemble, can be placed in incubators or hypoxic chambers, and accurately read high-density colony arrays of microorganisms.  We demonstrate the utility of PFIboxes using a time course gene expression approach, examining global Escherichia coli promoter activity using a fluorescent reporter library across a diverse panel of 15 antibiotics, each at several concentrations.  In all, PFIboxes provide an open solution to gene expression, for about 2 US dollars per treatment condition, including technical replicates.
PFIboxes
Printed fluorescence imaging boxes (PFIboxes) cost approximately 200 US dollars to assemble, can be placed in incubators or hypoxic chambers, and accurately read high-density colony arrays of microorganisms.  We demonstrate the utility of PFIboxes using a time course gene expression approach, examining global Escherichia coli promoter activity using a fluorescent reporter library across a diverse panel of 15 antibiotics, each at several concentrations.  In all, PFIboxes provide an open solution to gene expression, for about 2 US dollars per treatment condition, including technical replicates.
PFIboxes
Here, we present 3D-printed, open-source tools to acquire fluorescence phenotypes with high temporal resolution. 
3D-printed
Open-Source High-Throughput Phenomics of Bacterial Promoter-Reporter Strains.Open-source electronics are becoming more prevalent in biological sciences, enabling novel and unique means of data acquisition. 
open-source electronics
Printed fluorescence imaging boxes (PFIboxes) cost approximately 200 US dollars to assemble, can be placed in incubators or hypoxic chambers, and accurately read high-density colony arrays of microorganisms.  We demonstrate the utility of PFIboxes using a time course gene expression approach, examining global Escherichia coli promoter activity using a fluorescent reporter library across a diverse panel of 15 antibiotics, each at several concentrations.  In all, PFIboxes provide an open solution to gene expression, for about 2 US dollars per treatment condition, including technical replicates.
PFIboxes