Genotypes and allele frequencies were calculated using SPSS 16 software.



SPSS
Polymorphisms were genotyped by PCR-RFLP and the miRNA expression was measured using qRT-PCR. 
PCR-RFLP
Polymorphisms were genotyped by PCR-RFLP and the miRNA expression was measured using qRT-PCR. 
qRT-PCR