Estrogen Deficiency Leads to Further Bone Loss in the Mandible of CKD Mice.BACKGROUND
 Chronic kidney disease (CKD) has been regarded as a grave public health problem.  Our previous study demonstrated that CKD impairs the healing of titanium implants.  The aim of this study was to investigate the effects of estrogen deficiency on the mandibular bone in CKD mice.


 Serum measurements confirmed a significant increase in BUN in the CKD group that was further increased by OVX.  OVX led to significant decreases in both the BV/TV and cortical thickness of the mandibular bone in CKD mice.


 In summary, our findings indicate that estrogen deficiency leads to further mandibular bone loss in CKD mice.
CKD
Uremia and estrogen deficiency were induced by 5/6 nephrectomy and ovariectomy (OVX), respectively.  Serum measurements confirmed a significant increase in BUN in the CKD group that was further increased by OVX.  OVX led to significant decreases in both the BV/TV and cortical thickness of the mandibular bone in CKD mice.



OVX
After 8 weeks, the mice were sacrificed, and their mandibles were collected for micro-CT analysis and histological examination.



micro-CT analysis
After 8 weeks, the mice were sacrificed, and their mandibles were collected for micro-CT analysis and histological examination.



histological examination