NLRP3 mRNA expression was assessed by real time polymerase chain reaction. 
real time polymerase chain reaction
Serum 8-hydroxy-2'-deoxyguanosine (8-OHdG), interleukin 1β (IL-1β), and uHSP72 levels were estimated by enzyme-linked immunosorbent assay. 
enzyme-linked immunosorbent assay