Efficient TALEN-mediated gene targeting of chicken primordial germ cells.In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 (vasa) locus in chicken primordial germ cells (PGCs).  We show that TALENs mediate homology-directed repair of the DDX4 locus on the Z sex chromosome at high (8.1%) efficiencies.  Large genetic deletions of 30 kb encompassing the entire DDX4 locus were also created using a single TALEN pair.  TALEN-mediated gene targeting in avian PGCs is therefore an efficient process.
TALEN
Efficient TALEN-mediated gene targeting of chicken primordial germ cells.In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 (vasa) locus in chicken primordial germ cells (PGCs).  We show that TALENs mediate homology-directed repair of the DDX4 locus on the Z sex chromosome at high (8.1%) efficiencies. 
TALENs
Efficient TALEN-mediated gene targeting of chicken primordial germ cells.In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 (vasa) locus in chicken primordial germ cells (PGCs).  We show that TALENs mediate homology-directed repair of the DDX4 locus on the Z sex chromosome at high (8.1%) efficiencies.  Large genetic deletions of 30 kb encompassing the entire DDX4 locus were also created using a single TALEN pair.  TALEN-mediated gene targeting in avian PGCs is therefore an efficient process.
TALEN