Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks.Two-photon imaging in behaving animals has revealed neuronal activities related to behavioral and cognitive function at single-cell resolution.  Here we report the development of protocols to train head-fixed common marmosets to perform upper-limb movement tasks and simultaneously perform two-photon imaging. 
Two-photon imaging
Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks.Two-photon imaging in behaving animals has revealed neuronal activities related to behavioral and cognitive function at single-cell resolution.  Here we report the development of protocols to train head-fixed common marmosets to perform upper-limb movement tasks and simultaneously perform two-photon imaging. 
two-photon imaging
We conduct two-photon calcium imaging of layer 2/3 neurons in the motor cortex during this motor task performance, and detect task-relevant activity from multiple neurons at cellular and subcellular resolutions.  Two-photon calcium imaging in behaving marmosets may become a fundamental technique for determining the spatial organization of the cortical dynamics underlying action and cognition.
two-photon calcium imaging