Imaging transparent intact cardiac tissue with single-cell resolution.We developed a new method, SUT (Scheme Update on tissue Transparency), to view cardiac microstructures and unveil the molecular changes underlying cardiac diseases.  SUT is an effective method to clear whole-hearts from different species.  SUT, together with EAL and modern imaging techniques, were successfully used to visualize three-dimensional spatial distribution of various molecules in cardiac tissue. 
SUT
In addition, EAL (Electrophoretic Antibody Labeling) was used to achieve fast antibody labeling by electric force, which significantly reduced antibody incubation time from days to hours.  SUT, together with EAL and modern imaging techniques, were successfully used to visualize three-dimensional spatial distribution of various molecules in cardiac tissue.  We also observed changes in the number and phenotypes of fibroblasts during post-myocardial infarction in a stereoscopic pattern. 
EAL