High-throughput BioSorter quantification of relative mitochondrial content and membrane potential in living Caenorhabditis elegans.Mitochondrial respiratory chain disease is caused by a wide range of individually rare genetic disorders that impair cellular energy metabolism.  Relative fluorescence profiles of nematode populations co-labeled with MTG and TMRE were obtained and analyzed by BioSorter (Union Biometrica).  BioSorter analysis independently validated relative MTG and TMRE changes that we had previously performed by fluorescence microscopy in a variety of experimental conditions, with notably greater animal population sizes and substantially reduced experimental time.  Older, fragile animal populations that are difficult to study by microscopy approaches were readily amenable to analysis with the BioSorter method. 
BioSorter
A custom MATLAB script, which can be run on any computer with MATLAB runtime, was written to automatically quantify and analyze results in large animal populations. 
MATLAB