N-terminal truncation contributed to increasing thermal stability of mannanase Man1312 without activity loss.BACKGROUND
 To increase understanding of the influence of distal residue mutation, this study explored the thermostability and enzymatic activity of mannanase Man1312 deletion mutants.  The effects of N-terminal truncation on enzymatic activity and thermal dynamics were investigated by spectrophotometry, circular dichroism and differential scanning calorimetry assays.


 N-terminal truncation had positive effects on the thermostability and activity of mannanase.
THERM