Synthesis, antiproliferative and antimicrobial activity of new Mannich bases bearing 1,2,4-triazole moiety.Abstract This study presents the synthesis, antiproliferative and antimicrobial evaluation of a new series of Mannich base derivatives containing 1,2,4-triazole system.  New compounds were prepared by the reaction of 4,5-disubstituted 1,2,4-triazole-3-thiones with formaldehyde and various amines.  The structures of the prepared compounds were confirmed by means of (1)H NMR, (13)C NMR and elemental analyses.  Twelve compounds were evaluated for their in vitro antiproliferative activities against six chosen cancer cell lines.  All synthesized compounds were screened for their in vitro antimicrobial activity by using the agar dilution technique.  For 17 potentially active compounds, their antibacterial activity was confirmed on the basis of MIC (minimal inhibitory concentration) by broth microdilution method using the reference Gram-positive and Gram-negative bacterial strains.
C