Differential gene expression in ovaries of Qira black sheep and Hetian sheep using RNA-Seq technique.The Qira black sheep and the Hetian sheep are two local breeds in the Northwest of China, which are characterized by high-fecundity and low-fecundity breed respectively.  In the present study, we performed RNA-seq technology to compare the difference in ovarian mRNA expression profiles between Qira black sheep and Hetian sheep. 
RNA-seq
In the present study, we performed RNA-seq technology to compare the difference in ovarian mRNA expression profiles between Qira black sheep and Hetian sheep. 
RNA-seq technology
Eight differentially expressed genes were randomly selected for validation by real-time RT-PCR. 
real-time RT-PCR