Sulfated modification and anticoagulant activity of pumpkin (Cucurbita pepo, Lady Godiva) polysaccharide.Sulfated modification of pumpkin polysaccharide using CAS with pyridines as catalysts under different conditions was conducted to obtain different degrees of sulfation on a laboratory scale.  Results showed that addition of high ratio of CAS/pyridine under constant conditions could increase the degree of substitution. 
CAS
Sulfate substitution was further confirmed by the FT-IR and 13C NMR analysis. 
FT-IR
Sulfate substitution was further confirmed by the FT-IR and 13C NMR analysis. 
13C NMR