Vasohibin 2 as a potential predictor of aggressive behavior of triple-negative breast cancer.Triple-negative breast cancer (TNBC) is a subtype breast cancer with aggressive behavior, advanced disease status and poor prognosis.  Vasohibin 2 (VASH2) was previously identified as an angiogenic factor, but its role in TNBC tumorigenesis is unknown. 
Vasohibin 2
Using quantitative PCR and western blot analyses, we found that VASH2 is overexpressed in TNBC cells and tissues. 
quantitative PCR
Using quantitative PCR and western blot analyses, we found that VASH2 is overexpressed in TNBC cells and tissues. 
western blot analyses
Knockdown of VASH2 via siRNA inhibited the proliferation of the TNBC cell lines by delaying cell cycle progression and increasing apoptosis. 
siRNA
Further analyses showed that the VASH2-mediated increase in the transcription of fibroblast growth factor-2, vascular endothelial growth factor and vasohibin 1 may be the mechanism underlying these effects. 
Fibroblast growth factor-2
Further analyses showed that the VASH2-mediated increase in the transcription of fibroblast growth factor-2, vascular endothelial growth factor and vasohibin 1 may be the mechanism underlying these effects. 
vascular endothelial growth factor
Further analyses showed that the VASH2-mediated increase in the transcription of fibroblast growth factor-2, vascular endothelial growth factor and vasohibin 1 may be the mechanism underlying these effects. 
vasohibin 1