miR-34c-3p expression was evaluated by qPCR. 
qPCR
Cell viability was examined by MTT and proliferation by cell cycle analysis. 
MTT
Cell migration and invasion were tested using Transwells with/without Matrigel coating. 
Transwells
Western blot analysis was performed for eIF4E, c-Myc, Cyclin D1, survivin and Mcl-1 protein expression.



Western blot analysis
Target analysis using algorithms miRanda, TargetScan and DIANA identified eIF4E as a potential target of miR-34c-3p. 
miRanda
Target analysis using algorithms miRanda, TargetScan and DIANA identified eIF4E as a potential target of miR-34c-3p. 
TargetScan
Target analysis using algorithms miRanda, TargetScan and DIANA identified eIF4E as a potential target of miR-34c-3p. 
DIANA
Luciferase assay using the eIF4E 3'-UTR reporter carrying a putative miR-34c-3p target sequence revealed eIF4E to be a specific target of miR-34c-3p. 
Luciferase assay