bisFabs: Tools for rapidly screening hybridoma IgGs for their activities as bispecific antibodies.Bispecific antibodies are a growing class of therapeutic molecules.  We describe here a method to generate bispecific molecules from hybridoma IgGs in 3-4 d using chemical conjugation of antigen-binding fragments (Fabs) (bisFabs).  The resulting bisFabs showed no significant amounts of homodimers or aggregates.  The predictive value of murine bisFabs was tested by comparing the T-cell redirected cytotoxic activity of a panel of antibodies in either the bisFab or full-length IgG formats.  The activity observed for different murine bisFabs correlated with those observed for the full-length IgG format across multiple different antigen targets, supporting the use of bisFabs as a screening tool. 
bisFabs
bisFabs: Tools for rapidly screening hybridoma IgGs for their activities as bispecific antibodies.Bispecific antibodies are a growing class of therapeutic molecules.  We describe here a method to generate bispecific molecules from hybridoma IgGs in 3-4 d using chemical conjugation of antigen-binding fragments (Fabs) (bisFabs).  The resulting bisFabs showed no significant amounts of homodimers or aggregates.  The predictive value of murine bisFabs was tested by comparing the T-cell redirected cytotoxic activity of a panel of antibodies in either the bisFab or full-length IgG formats.  The activity observed for different murine bisFabs correlated with those observed for the full-length IgG format across multiple different antigen targets, supporting the use of bisFabs as a screening tool. 
bisFabs