Cell growth was measured in Matrigel™ 3D culture. 
Matrigel
Cytokine/chemokine concentrations in tumor tissue and plasma were measured by multiplexing laser bead technology. 
multiplexing laser bead technology
The expression of IL-6 in breast cancer cell lines was determined by RT-PCR. 
RT-PCR
The effects of doxycycline on NF-κB-dependent signaling were analyzed by Western blotting.



Western blotting