However, it is not clear whether MTX remediates SCI by inhibiting ERS.  Furthermore, the in vivo role of MTX in a rat model of SCI was evaluated.  Furthermore, levels of ERS-associated proteins, including caspase-3, activating transcription factor 6, serine/threonine-protein kinase/endoribonuclease inositol-requiring enzyme 1 α, eukaryotic initiation factor 2 α and GRP78, were significantly increased following SCI; however, administration of MTX for 7 days significantly reversed this effect (P<0.05, P<0.01 and P<0.001). 
THERM