Transcriptional Profiling of Newly Generated Dentate Granule Cells Using TU Tagging Reveals Pattern Shifts in Gene Expression during Circuit Integration.Despite representing only a small fraction of hippocampal granule cells, adult-generated newborn granule cells have been implicated in learning and memory (Aimone et al., 2011). 
TU tagging
Subsequent injection of 4-TU tagged nascent RNAs for analysis by RNA sequencing. 
RNA sequencing
We compared a selection of the enriched genes with steady-state levels of mRNAs using quantitative PCR. 
quantitative PCR