A rapid, efficient, and economical inverse polymerase chain reaction-based method for generating a site saturation mutant library.With the development of deep sequencing methodologies, it has become important to construct site saturation mutant (SSM) libraries in which every nucleotide/codon in a gene is individually randomized.  We describe methodologies for the rapid, efficient, and economical construction of such libraries using inverse polymerase chain reaction (PCR). 
inverse polymerase chain reaction
A rapid, efficient, and economical inverse polymerase chain reaction-based method for generating a site saturation mutant library.With the development of deep sequencing methodologies, it has become important to construct site saturation mutant (SSM) libraries in which every nucleotide/codon in a gene is individually randomized. 
deep sequencing methodologies