The contents of chlorogenic acid (CGA), caffeic acid (CA), and luteolin in FL were detected by high-performance liquid chromatography (HPLC).


 Furthermore, the results of HPLC demonstrated that the amount of CGA was the highest in FL.



HPLC
Enzyme-linked immunosorbent assay (ELISA) was used to detect serum vascular endothelial growth factor (VEGF) content. 
ELISA