Colonization and subsequent tissue dissemination was followed over a 24-h period using the IVIS spectrum imaging workstation. 
IVIS spectrum imaging workstation
A virulent Danish A. salmonicida strain was tagged with pAKgfplux1, a dual-labelled plasmid vector containing the mutated gfpmut3a gene from Aequorea victoria and the luxCDABE genes from the bacterium Photorhabdus luminescens.  Fish were infected with pAKgfplux1 tagged A. salmonicida via immersion bath. 
pAKgfplux1