Application of Zebrafish and Knockdown Technology to Define Progranulin Neuronal Function.The zebrafish (Danio rerio), a small tropical fish, has become a powerful model for the study of early vertebrate development, human diseases, and drug screening.  Zebrafish provides large numbers of optically clear embryos, and its development is very rapid.  We defined the expression pattern of zebrafish progranulin A (zfPGRN-A), an orthologue to the single human PGRN by whole-mount in situ hybridization (ISH) and immunofluorescence (IF). 
Zebrafish
Application of Zebrafish and Knockdown Technology to Define Progranulin Neuronal Function.The zebrafish (Danio rerio), a small tropical fish, has become a powerful model for the study of early vertebrate development, human diseases, and drug screening. 
Knockdown Technology