Bioinformatics software applications were utilized to exploit statistical algorithms to demonstrate protein structure and variants conservation. 
Bioinformatics software applications
Novel candidate genes may be possible predisposing factors revealed by whole exome sequencing in familial esophageal squamous cell carcinoma.Esophageal squamous cell carcinoma is one of the deadliest of all the cancers.  A more advanced method to identify new molecular biomarkers predicting disease prognosis can be whole exome sequencing.  Here, we report the most effective genetic variants of the Notch signaling pathway in esophageal squamous cell carcinoma susceptibility by whole exome sequencing.  Genomic DNA was extracted and whole exome sequencing performed to generate information about genetic variants in the coding regions. 
whole exome sequencing