DeepSAGE based differential gene expression analysis under cold and freeze stress in seabuckthorn (Hippophae rhamnoides L.). We employed DeepSAGE, a tag based approach, to identify differentially expressed genes under cold and freeze stress in seabuckthorn.  Expression of randomly selected 22 DEGs was validated using qRT-PCR that further supported our DeepSAGE results.  The DeepSAGE data could also serve as a valuable resource for further functional genomics studies aiming selection of candidate genes for development of abiotic stress tolerant transgenic plants.
DeepSAGE
In total 36.2 million raw tags including 13.9 million distinct tags were generated using Illumina sequencing platform for three leaf tissue libraries including control (CON), cold stress (CS) and freeze stress (FS). 
Illumina sequencing platform
Expression of randomly selected 22 DEGs was validated using qRT-PCR that further supported our DeepSAGE results. 
qRT-PCR