PrimerMapper: high throughput primer design and graphical assembly for PCR and SNP detection.Primer design represents a widely employed gambit in diverse molecular applications including PCR, sequencing, and probe hybridization.  To this end, I describe here, PrimerMapper, which provides a comprehensive graphical user interface that designs robust primers from any number of inputted sequences while providing the user with both, graphical maps of primer distribution for each inputted sequence, and also a global assembled map of all inputted sequences with designed primers.  PrimerMapper also enables the visualization of graphical maps within a browser and allows the user to draw new primers directly onto the webpage.  Other features of PrimerMapper include allele-specific design features for SNP genotyping, a remote BLAST window to NCBI databases, and remote sequence retrieval from GenBank and dbSNP.  PrimerMapper is hosted at GitHub and freely available without restriction.
PrimerMapper
PrimerMapper: high throughput primer design and graphical assembly for PCR and SNP detection.Primer design represents a widely employed gambit in diverse molecular applications including PCR, sequencing, and probe hybridization.  To this end, I describe here, PrimerMapper, which provides a comprehensive graphical user interface that designs robust primers from any number of inputted sequences while providing the user with both, graphical maps of primer distribution for each inputted sequence, and also a global assembled map of all inputted sequences with designed primers.  PrimerMapper also enables the visualization of graphical maps within a browser and allows the user to draw new primers directly onto the webpage.  Other features of PrimerMapper include allele-specific design features for SNP genotyping, a remote BLAST window to NCBI databases, and remote sequence retrieval from GenBank and dbSNP.  PrimerMapper is hosted at GitHub and freely available without restriction.
PrimerMapper