Cytoplasmic protein, mitochondria and mitochondrial proteins were extracted from the myocardial tissue, and other myocardial tissue was frozen for next analysis.  Furthermore, the levels of autophagy-related proteins such as microtubule-associated protein 1 light chain 3 (LC3), PTEN-induced kinase 1 (pink1), E3-ubiquitin ligase parkin were measured by Western blotting and fluorescent immunohistochemistry.



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