Body: <p>It is my first time using Chimera. I have uploaded a PDB file on to Chimera and opened it up on the software. I need to change the threshold of the EM map so that I can analyze what the EM map would look like with a threshold too high and too low.</p>
  <p>Please let me know if you have any suggestions.</p>
  <p>Thanks</p>
  
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2022-02-11T14:22:25.007Z
Comments.Text: Could you supply more details on the goal of your analysis?
Comments.UserDisplayName: M__
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2022-02-11T14:22:39.363Z
Comments.Text: https://www.cgl.ucsf.edu/chimera/current/docs/ContributedSoftware/volumeviewer/framevolumeviewer.html
Comments.UserDisplayName: pippo1980
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2022-02-11T14:32:18.127Z
Comments.Text: I am trying to view a protein with a threshold that Is too high, a threshold that is too low, and finally, the correct threshold to se the secondary structures.
Comments.UserDisplayName: microbeprincess
Tags: <proteins><protein-structure>
Title: Chimera- How to go about changing the threshold of the EM map?
ThreadText: # Chimera- How to go about changing the threshold of the EM map?
  
  (Asked by: username_0 on 2022-02-11)
  
  It is my first time using Chimera. I have uploaded a PDB file on to Chimera and opened it up on the software. I need to change the threshold of the EM map so that I can analyze what the EM map would look like with a threshold too high and too low.
  
  Please let me know if you have any suggestions.
  
  Thanks
  
  **Tags:** proteins, protein-structure
  
  > **Comments:**
  > **username_1**: Could you supply more details on the goal of your analysis?  
  > **username_2**: https://www.cgl.ucsf.edu/chimera/current/docs/ContributedSoftware/volumeviewer/framevolumeviewer.html  
  > **username_0**: I am trying to view a protein with a threshold that Is too high, a threshold that is too low, and finally, the correct threshold to se the secondary structures.  
  
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  ## 0 answers
  
  