Answers.Body: <p>Yes, just add up the coverage at each location; that's integration in a nutshell.</p>
  
  <p>Or, alternatively / equivalently, count the total number of reads within the region.</p>
  
Answers.ContentLicense: CC BY-SA 4.0
Answers.CreationDate: 2018-10-16T21:16:25.643Z
Answers.LastActivityDate: 2018-10-16T21:16:25.643Z
Answers.UserDisplayName: gringer
Body: <p>Is there a way to calculate the area under a curve in a coverage graph?</p>
  
  <p>Thank you in advance.</p>
  
  <p>EDIT:</p>
  
  <p>I'd like to calculate the AUC per strand per gene in a coverage graph. I have two wig files (sense and antisense) generated from a single bam (RNA-seq) file (using the <a href="https://github.com/pmenzel/stranded-coverage" rel="nofollow noreferrer">strand-coverage</a> software).</p>
  
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2018-10-17T14:16:37.643Z
Comments.Text: Welcome to the group.  Can you expand on your question to include what tools you are using, what you have tried.  How did you generate the curve and do you have any sample data to help answer your question?
Comments.UserDisplayName: Bioathlete
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2018-10-17T15:22:19.187Z
Comments.Text: Hi Bioathlete. I'm totally new in Bioinformatics field. I have not tried much yet due to lack of knowledge, but I edited my question. Thank you.
Comments.UserDisplayName: Guzto
Tags: <rna-seq><coverage>
Title: How to calculate AUC in coverage graph
ThreadText: # How to calculate AUC in coverage graph
  
  (Asked by: username_0 on 2018-10-16)
  
  Is there a way to calculate the area under a curve in a coverage graph?
  
  Thank you in advance.
  
  EDIT:
  
  I'd like to calculate the AUC per strand per gene in a coverage graph. I have two wig files (sense and antisense) generated from a single bam (RNA-seq) file (using the [strand-coverage](https://github.com/pmenzel/stranded-coverage) software).
  
  **Tags:** rna-seq, coverage
  
  > **Comments:**
  > **username_1**: Welcome to the group.  Can you expand on your question to include what tools you are using, what you have tried.  How did you generate the curve and do you have any sample data to help answer your question?  
  > **username_0**: Hi username_1. I'm totally new in Bioinformatics field. I have not tried much yet due to lack of knowledge, but I edited my question. Thank you.  
  
  ---
  
  ## 1 answers
  
  ### Answer by username_2 on 2018-10-16. Score: 4 | Accepted answer
  
  Yes, just add up the coverage at each location; that's integration in a nutshell.
  
  Or, alternatively / equivalently, count the total number of reads within the region.
  
  
  ---
  
  