Answers.Body: <p><em>Answer from @haci, converted from comment:</em></p>
  <p>You should run your command within that directory (the directory where the fastq file is), this does not seem to be the case as far as I see. Alternatively you can provide the absolute path of your fastq file.</p>
  <p><code>cd</code> into the directory where the fastq file is (you should be able to see your file of interest when you type <code>ls</code>) and then run the command in your question:</p>
  <pre><code>cd ./genome_diirt
  seqtk seq -a KF0124_1_1.fastq &gt; KF0124_1_1.fasta
  </code></pre>
  <p>Alternatively you can provide the absolute path of your fastq file:</p>
  <pre><code>seqtk seq -a ./genome_diirt/KF0124_1_1.fastq &gt; ./genome_diirt/KF0124_1_1.fasta
  </code></pre>
  
Answers.ContentLicense: CC BY-SA 4.0
Answers.CreationDate: 2021-07-23T21:47:52.403Z
Answers.LastActivityDate: 2021-07-23T21:47:52.403Z
Answers.UserDisplayName: gringer
Body: <p>I am new to bioinformatics and programming. I tried to convert my fastq files to fasta file, but got this. What could be wrong?</p>
  <pre><code>(base) jszheng@Greater:~/seqtk$ seqtk seq -a KF0124_1_1.fastq &gt; KF0124_1_1.fasta
  [E::stk_seq] failed to open the input file/stream.
  </code></pre>
  
Tags: <fasta><genome><sequence-analysis><fastq><linux>
Title: Converting Fastq files to Fasta files on Ubuntu
ThreadText: # Converting Fastq files to Fasta files on Ubuntu
  
  (Asked by: username_0 on 2021-07-23)
  
  I am new to bioinformatics and programming. I tried to convert my fastq files to fasta file, but got this. What could be wrong?
  
  ```
  (base) jszheng@username_0:~/seqtk$ seqtk seq -a KF0124_1_1.fastq > KF0124_1_1.fasta
  [E::stk_seq] failed to open the input file/stream.
  
  ```
  
  **Tags:** fasta, genome, sequence-analysis, fastq, linux
  
  
  ---
  
  ## 1 answers
  
  ### Answer by username_1 on 2021-07-23. Score: 2
  
  *Answer from @haci, converted from comment:*
  
  You should run your command within that directory (the directory where the fastq file is), this does not seem to be the case as far as I see. Alternatively you can provide the absolute path of your fastq file.
  
  `cd` into the directory where the fastq file is (you should be able to see your file of interest when you type `ls`) and then run the command in your question:
  
  ```
  cd ./genome_diirt
  seqtk seq -a KF0124_1_1.fastq > KF0124_1_1.fasta
  
  ```
  
  Alternatively you can provide the absolute path of your fastq file:
  
  ```
  seqtk seq -a ./genome_diirt/KF0124_1_1.fastq > ./genome_diirt/KF0124_1_1.fasta
  
  ```
  
  
  ---
  
  