Body: <p>I am planning to perform multiple routine NGS analyses for a hospital lab on patients' samples on an everyday basis.</p>
  <p>Before I used a new tuxedo approach with <code>ballgown</code> etc and was rather satisfied with it.</p>
  <p>I wonder if for the above-mentioned job it's ok to use a ballgown or, perhaps, that is not the best choice?</p>
  
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2021-06-21T15:45:26.010Z
Comments.Text: I'm fairly used to analyse NGS illumina data, but what is the New Tuxedo approach you mention? How do you measure best choice?
Comments.UserDisplayName: llrs
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2021-06-23T12:53:42.513Z
Comments.Text: Thanks I am talking about this approach https://www.nature.com/articles/nprot.2016.095
Comments.UserDisplayName: Mark
Tags: <bioconductor>
Title: Is New Tuxedo approach really better than the earlier ones?
ThreadText: # Is New Tuxedo approach really better than the earlier ones?
  
  (Asked by: username_0 on 2021-06-21)
  
  I am planning to perform multiple routine NGS analyses for a hospital lab on patients' samples on an everyday basis.
  
  Before I used a new tuxedo approach with `ballgown` etc and was rather satisfied with it.
  
  I wonder if for the above-mentioned job it's ok to use a ballgown or, perhaps, that is not the best choice?
  
  **Tags:** bioconductor
  
  > **Comments:**
  > **username_1**: I'm fairly used to analyse NGS illumina data, but what is the New Tuxedo approach you mention? How do you measure best choice?  
  > **username_0**: Thanks I am talking about this approach https://www.nature.com/articles/nprot.2016.095  
  
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  ## 0 answers
  
  