Body: <p>I have done a study on isoform switch between different tissue type. Now, many people asking me to study alternative splicing also. But, dont they will give me same results? If there is a significant splicing difference; that will definitely leads to isoform switch. Or my understanding is incorrect ?</p>
  
  <p>Thanks</p>
  
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2020-05-23T22:02:05.817Z
Comments.Text: Imaging isoform A uses exon 1, and isoform B uses exon 2. Now if you do an isoform switch analysis, you might see that in one condition isoform A (and exon 1) is preferred over isoform B (and exon 2). You might get similar results for alternative splicing. But if there is another isoform C that uses both exon 1 and 2, then the alternative splicing analysis of these exons are going to be affected by the expression level of isoform C as well.
Comments.UserDisplayName: Phoenix Mu
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2020-06-07T02:55:23.863Z
Comments.Text: Thanks @PhoenixMu
Comments.UserDisplayName: user3377241
Tags: <differential-expression><rna-splicing><isoform>
Title: Do I need to study alternative splicing and isoformswitch separately?
ThreadText: # Do I need to study alternative splicing and isoformswitch separately?
  
  (Asked by: username_0 on 2020-05-23)
  
  I have done a study on isoform switch between different tissue type. Now, many people asking me to study alternative splicing also. But, dont they will give me same results? If there is a significant splicing difference; that will definitely leads to isoform switch. Or my understanding is incorrect ?
  
  Thanks
  
  **Tags:** differential-expression, rna-splicing, isoform
  
  > **Comments:**
  > **username_1**: Imaging isoform A uses exon 1, and isoform B uses exon 2. Now if you do an isoform switch analysis, you might see that in one condition isoform A (and exon 1) is preferred over isoform B (and exon 2). You might get similar results for alternative splicing. But if there is another isoform C that uses both exon 1 and 2, then the alternative splicing analysis of these exons are going to be affected by the expression level of isoform C as well.  
  > **username_0**: Thanks @username_1  
  
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  ## 0 answers
  
  