Answers.Body: <p>Yes, Prokka will consider all six-frames, the gene prediction is actually done with Prodigal, paper:
  <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848648/" rel="noreferrer">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848648/</a></p>
  
  <p>github:
  <a href="https://github.com/hyattpd/Prodigal" rel="noreferrer">https://github.com/hyattpd/Prodigal</a></p>
  
Answers.ContentLicense: CC BY-SA 4.0
Answers.CreationDate: 2018-12-27T21:42:52.467Z
Answers.LastActivityDate: 2018-12-27T21:42:52.467Z
Answers.UserDisplayName: Chris_Rands
Body: <p>Does Prokka do six-frame genome annotations? The corresponding publication does not mention it.</p>
  
  <blockquote>
    <p>Seemann, Torsten. 2014. “Prokka: Rapid Prokaryotic Genome Annotation.” Bioinformatics  30 (14): 2068–69.</p>
  </blockquote>
  
Tags: <sequence-annotation>
Title: Does Prokka do six-frame translations?
ThreadText: # Does Prokka do six-frame translations?
  
  (Asked by: username_0 on 2018-12-27)
  
  Does Prokka do six-frame genome annotations? The corresponding publication does not mention it.
  
  > Seemann, Torsten. 2014. “Prokka: Rapid Prokaryotic Genome Annotation.” Bioinformatics 30 (14): 2068–69.
  
  **Tags:** sequence-annotation
  
  
  ---
  
  ## 1 answers
  
  ### Answer by username_1 on 2018-12-27. Score: 7 | Accepted answer
  
  Yes, Prokka will consider all six-frames, the gene prediction is actually done with Prodigal, paper:
  <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848648/>
  
  github:
  <https://github.com/hyattpd/Prodigal>
  
  
  ---
  
  