Answers.Body: <p>PacBio lima need both 5'end and 3' end barcodes. You can have a unique barcode in one end and multiple on the other end; but it need two barcodes.</p>
  
Answers.ContentLicense: CC BY-SA 4.0
Answers.CreationDate: 2024-01-03T01:04:31.737Z
Answers.LastActivityDate: 2024-01-03T01:04:31.737Z
Answers.UserDisplayName: user3377241
Body: <p>I was wondering if there is a way to provide pacbio lima demultiplexing only a barcode, and let  lima demux sample even if only one end has the barcode, not both?</p>
  
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2023-12-21T09:08:36.750Z
Comments.Text: Can you please explain more about the context of your problem? What are you trying to do with the demultiplexing; what is the ultimate aim of the analysis?
Comments.UserDisplayName: gringer
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2023-12-21T19:00:06.197Z
Comments.Text: Some examples of what your data look like might help
Comments.UserDisplayName: Maximilian Press
Tags: <pacbio><demultiplexing>
Title: pacbio demultiplexing issue
ThreadText: # pacbio demultiplexing issue
  
  (Asked by: username_0 on 2023-12-19)
  
  I was wondering if there is a way to provide pacbio lima demultiplexing only a barcode, and let lima demux sample even if only one end has the barcode, not both?
  
  **Tags:** pacbio, demultiplexing
  
  > **Comments:**
  > **username_1**: Can you please explain more about the context of your problem? What are you trying to do with the demultiplexing; what is the ultimate aim of the analysis?  
  > **username_2**: Some examples of what your data look like might help  
  
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  ## 1 answers
  
  ### Answer by username_3 on 2024-01-03. Score: -1
  
  PacBio lima need both 5'end and 3' end barcodes. You can have a unique barcode in one end and multiple on the other end; but it need two barcodes.
  
  
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