Body: <p>I'm a newcomer to the field of antibodies, and I'm seeking guidance on obtaining 3D structures for my molecular simulations. After browsing the PDB databank, I noticed that there are Fab and Fc structures available. My specific interest lies in investigating the structural dynamics in the entire antibody (AB) and on the hinge region.</p>
  <p>I have a few questions:</p>
  <ol>
  <li><p>Is it common practice to attempt building the entire antibody structure and hinge regions from PDB files for fragments, or are there recommended alternatives?</p>
  </li>
  <li><p>Are there comprehensive PDB structures available for different IgG and therapeutic antibodies that include information on the whole structure and hinge regions? Especially for Bevacizumab and Rituximab?</p>
  </li>
  <li><p>Does it make sense to select single segments, e.g., CH1, VH, VL, for analysis? Would one expect these structures to be reasonably stable?</p>
  </li>
  </ol>
  <p>Additionally, I'd appreciate any insights into common pitfalls and any recommendations you might have for someone new to this field.</p>
  <p>Thank you in advance for your time and assistance.</p>
  <p>Best regards,
  Albert</p>
  
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2023-11-20T21:11:52.027Z
Comments.Text: Please edit the question to limit it to a specific problem with enough detail to identify an adequate answer.
Comments.UserDisplayName: Community
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2023-11-21T07:21:59.403Z
Comments.Text: Hi, if you've got multiple questions, it's often better to split them up into separate question posts. However, exploratory posts like this are almost always going to produce better outcomes on discussion websites like [reddit](https://reddit.com/r/bioinformatics/submit)
Comments.UserDisplayName: gringer
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2023-11-21T19:56:59.670Z
Comments.Text: wait for cryomicroscopist to deliver https://www.nature.com/articles/s41467-022-34090-2  Here, we use cryo-EM to image the structure of the human full-length IgM pentamer, r
Comments.UserDisplayName: pippo1980
Tags: <pdb><structural-biology>
Title: (Complete) Stuctural Models of Antibodies: Combining Fc and Fab from pdb to Build Hinge Region? Or what?
ThreadText: # (Complete) Stuctural Models of Antibodies: Combining Fc and Fab from pdb to Build Hinge Region? Or what?
  
  (Asked by: username_0 on 2023-11-20)
  
  I'm a newcomer to the field of antibodies, and I'm seeking guidance on obtaining 3D structures for my molecular simulations. After browsing the PDB databank, I noticed that there are Fab and Fc structures available. My specific interest lies in investigating the structural dynamics in the entire antibody (AB) and on the hinge region.
  
  I have a few questions:
  
  1. Is it common practice to attempt building the entire antibody structure and hinge regions from PDB files for fragments, or are there recommended alternatives?
  2. Are there comprehensive PDB structures available for different IgG and therapeutic antibodies that include information on the whole structure and hinge regions? Especially for Bevacizumab and Rituximab?
  3. Does it make sense to select single segments, e.g., CH1, VH, VL, for analysis? Would one expect these structures to be reasonably stable?
  
  Additionally, I'd appreciate any insights into common pitfalls and any recommendations you might have for someone new to this field.
  
  Thank you in advance for your time and assistance.
  
  Best regards,
  Albert
  
  **Tags:** pdb, structural-biology
  
  > **Comments:**
  > **username_1**: Please edit the question to limit it to a specific problem with enough detail to identify an adequate answer.  
  > **username_2**: Hi, if you've got multiple questions, it's often better to split them up into separate question posts. However, exploratory posts like this are almost always going to produce better outcomes on discussion websites like [reddit](https://reddit.com/r/bioinformatics/submit)  
  > **username_3**: wait for cryomicroscopist to deliver https://www.nature.com/articles/s41467-022-34090-2  Here, we use cryo-EM to image the structure of the human full-length IgM pentamer, r  
  
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  ## 0 answers
  
  