Answers.Body: <p>Sequencing also requires single stranded DNA, otherwise the sequencing primers can’t bind. Sure there are steps in the protocol where double stranded material exists, but that’s transient. </p>
  
Answers.ContentLicense: CC BY-SA 4.0
Answers.CreationDate: 2018-05-20T06:47:44.827Z
Answers.LastActivityDate: 2018-05-20T06:47:44.827Z
Answers.UserDisplayName: Devon Ryan
Body: <p>Single stranded cDNA is used by race pcr and rt-pcr while next-gen sequencing (Illumina) requires double stranded cDNA. What is the reason behind this ? </p>
  
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2018-05-22T13:27:04.923Z
Comments.Text: I'm voting to close this question as off-topic because the question is not about bioinformatics, but for some specific methods of the wet lab. If you have a question about how to handle these sequences by computers, I think it would be better suited for the site.
Comments.UserDisplayName: llrs
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2018-05-22T14:41:19.513Z
Comments.Text: Can we migrate this to biology? It's a valid question and I think it's even somewhat relevant to bioinformatic (it's important to understand generation of the data), but it would suit better to Biology.SE I think. Anyway, could you link source of your information?
Comments.UserDisplayName: Kamil S Jaron
Tags: <phylogenetics>
Title: single stranded cDNA vs double-stranded cDNA
ThreadText: # single stranded cDNA vs double-stranded cDNA
  
  (Asked by: username_0 on 2018-05-19)
  
  Single stranded cDNA is used by race pcr and rt-pcr while next-gen sequencing (Illumina) requires double stranded cDNA. What is the reason behind this ?
  
  **Tags:** phylogenetics
  
  > **Comments:**
  > **username_1**: I'm voting to close this question as off-topic because the question is not about bioinformatics, but for some specific methods of the wet lab. If you have a question about how to handle these sequences by computers, I think it would be better suited for the site.  
  > **username_2**: Can we migrate this to biology? It's a valid question and I think it's even somewhat relevant to bioinformatic (it's important to understand generation of the data), but it would suit better to Biology.SE I think. Anyway, could you link source of your information?  
  
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  ## 1 answers
  
  ### Answer by username_3 on 2018-05-20. Score: 1 | Accepted answer
  
  Sequencing also requires single stranded DNA, otherwise the sequencing primers can’t bind. Sure there are steps in the protocol where double stranded material exists, but that’s transient.
  
  
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