Body: <p>I am in need to benchmark the variant identification pipeline which uses RNA seq data alone without any matched-normal. I would like to know the reference dataset (and the pipeline on which the dataset was run) that can I use to compare the pipeline results on my dataset. The reference dataset which I use should be comparable regardless of the dataset specific to certain disease. Thanks all in advance for your responses.</p>
  
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2022-10-20T07:57:32.770Z
Comments.Text: Please clarify your specific problem or provide additional details to highlight exactly what you need. As it's currently written, it's hard to tell exactly what you're asking.
Comments.UserDisplayName: Community
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2022-10-20T10:07:21.660Z
Comments.Text: Well, what is your dataset? What species? What kind of sample? You mention the absence of a matched normal, does that mean your sample is from a tumor? We need to understand what you have in order to help you validate.
Comments.UserDisplayName: terdon
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2022-10-21T08:57:17.863Z
Comments.Text: The samples come from human related to diabetic condition
Comments.UserDisplayName: Ahkam
Tags: <rna-seq><vcf><variant-calling><variation><benchmarking>
Title: Benchmarking for variant identification using RNA-seq data
ThreadText: # Benchmarking for variant identification using RNA-seq data
  
  (Asked by: username_0 on 2022-10-20)
  
  I am in need to benchmark the variant identification pipeline which uses RNA seq data alone without any matched-normal. I would like to know the reference dataset (and the pipeline on which the dataset was run) that can I use to compare the pipeline results on my dataset. The reference dataset which I use should be comparable regardless of the dataset specific to certain disease. Thanks all in advance for your responses.
  
  **Tags:** rna-seq, vcf, variant-calling, variation, benchmarking
  
  > **Comments:**
  > **username_1**: Please clarify your specific problem or provide additional details to highlight exactly what you need. As it's currently written, it's hard to tell exactly what you're asking.  
  > **username_2**: Well, what is your dataset? What species? What kind of sample? You mention the absence of a matched normal, does that mean your sample is from a tumor? We need to understand what you have in order to help you validate.  
  > **username_0**: The samples come from human related to diabetic condition  
  
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  ## 0 answers
  
  