Answers.Body: <p>With at least three samples, you can determine this by looking at the IBS0 output column (from either plink2 --make-king-table or KING): if the relationship in question has a very low IBS0 value compared to the other pairs, that's parent-child.  You can try to do the same thing with only two samples, but with no other IBS0 values to compare against it's easier to misjudge.</p>
  
Answers.ContentLicense: CC BY-SA 4.0
Answers.CreationDate: 2019-06-25T23:44:48.063Z
Answers.LastActivityDate: 2019-06-25T23:44:48.063Z
Answers.UserDisplayName: Christopher Chang
Body: <p>I am analyzing a family-based whole-genome sequence (WGS) dataset.  My identity-by-descent(IBD) analysis shows they share about 50% of their DNA. I think my pedigree structure may have gotten mixed up though and I want to see if one individual is the mother or the sister of a male individual.  Is there anyway to do this either with Plink or <a href="http://people.virginia.edu/~wc9c/KING/manual.html" rel="nofollow noreferrer">KING</a>? </p>
  
Tags: <wgs><plink>
Title: Sibling vs Parent/Child relationship detection using whole-genome sequence data
ThreadText: # Sibling vs Parent/Child relationship detection using whole-genome sequence data
  
  (Asked by: username_0 on 2019-06-24)
  
  I am analyzing a family-based whole-genome sequence (WGS) dataset. My identity-by-descent(IBD) analysis shows they share about 50% of their DNA. I think my pedigree structure may have gotten mixed up though and I want to see if one individual is the mother or the sister of a male individual. Is there anyway to do this either with Plink or [KING](http://people.virginia.edu/~wc9c/KING/manual.html)?
  
  **Tags:** wgs, plink
  
  
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  ## 1 answers
  
  ### Answer by username_1 on 2019-06-25. Score: 1 | Accepted answer
  
  With at least three samples, you can determine this by looking at the IBS0 output column (from either plink2 --make-king-table or KING): if the relationship in question has a very low IBS0 value compared to the other pairs, that's parent-child. You can try to do the same thing with only two samples, but with no other IBS0 values to compare against it's easier to misjudge.
  
  
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