Body: <p>How do you verify if a RNAseq dataset is unstranded, stranded or revesely stranded from a fastq file?</p>
  
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2021-01-27T09:15:15.067Z
Comments.Text: I was looking for a method that didn't involve quasi-mapping but I think this will solve my problem. Thank you!
Comments.UserDisplayName: indigoblueraspberry
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2021-01-27T09:18:09.283Z
Comments.Text: Unless the metadata is recorded somewhere you won’t get around mapping: the FASTQs themselves don’t record this information anywhere.
Comments.UserDisplayName: Konrad Rudolph
Tags: <rna-seq><fastq>
Title: How do you determine the strandedness of a RNAseq dataset?
ThreadText: # How do you determine the strandedness of a RNAseq dataset?
  
  (Asked by: username_0 on 2021-01-26)
  
  How do you verify if a RNAseq dataset is unstranded, stranded or revesely stranded from a fastq file?
  
  **Tags:** rna-seq, fastq
  
  > **Comments:**
  > **username_0**: I was looking for a method that didn't involve quasi-mapping but I think this will solve my problem. Thank you!  
  > **username_1**: Unless the metadata is recorded somewhere you won’t get around mapping: the FASTQs themselves don’t record this information anywhere.  
  
  ---
  
  ## 0 answers
  
  