Answers.Body: <pre><code>your_new_object &lt;- subset(your_object, subset = mapping.score &gt; 0.5)
  </code></pre>
  
Answers.ContentLicense: CC BY-SA 4.0
Answers.CreationDate: 2021-07-16T10:56:42.823Z
Answers.LastActivityDate: 2021-07-16T10:56:42.823Z
Answers.UserDisplayName: haci
Body: <p>I metadata slot of Seurat object I have mapping score of each cell to a reference PBMC data like</p>
  <pre><code>pbmc &lt;- subset(object, subset = object@meta.data$mapping.score &gt; 0.5)
  Error in FetchData(object = object, vars = unique(x = expr.char[vars.use]),  : 
    None of the requested variables were found: 
  &gt; 
  &gt; 
  &gt; 
  </code></pre>
  <p>I want to only keep cells with mapping score &gt; 0.5</p>
  <p>Any help please?</p>
  
Tags: <scrnaseq><seurat><10x-genomics>
Title: Filtering cells from values in metadata
ThreadText: # Filtering cells from values in metadata
  
  (Asked by: username_0 on 2021-07-16)
  
  I metadata slot of Seurat object I have mapping score of each cell to a reference PBMC data like
  
  ```
  pbmc <- subset(object, subset = object@meta.data$mapping.score > 0.5)
  Error in FetchData(object = object, vars = unique(x = expr.char[vars.use]),  : 
    None of the requested variables were found: 
  > 
  > 
  > 
  
  ```
  
  I want to only keep cells with mapping score > 0.5
  
  Any help please?
  
  **Tags:** scrnaseq, seurat, 10x-genomics
  
  
  ---
  
  ## 1 answers
  
  ### Answer by username_1 on 2021-07-16. Score: 1
  
  ```
  your_new_object <- subset(your_object, subset = mapping.score > 0.5)
  
  ```
  
  
  ---
  
  