Body: <p>I have two NGS files from an NGS company corresponding to the sequencing data from a tumor sample as follows:</p>
  <ul>
  <li>TB_7710391_R1.FASTQ.gz</li>
  <li>TB_7710391_R2.FASTQ.gz</li>
  </ul>
  <p>I have downloaded the genome for MCPyV as a file called “MCPYV_Sequence.fasta”</p>
  <p>I have downloaded the Hg38 as a file called “hg_38.fa”</p>
  <p>I have input the host genome as hg_38.fa, the viral genome as “ MCPYV_Sequence.fasta” and the sequence data as both FASTQ files.</p>
  <p>I run the assembly in the cloud, and I receive back an error stating that the assembly as failed.</p>
  <p>What am I doing wrong?</p>
  
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2023-02-11T23:28:07.307Z
Comments.Text: Please clarify your specific problem or provide additional details to highlight exactly what you need. As it's currently written, it's hard to tell exactly what you're asking.
Comments.UserDisplayName: Community
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2023-02-12T19:19:04.763Z
Comments.Text: This question needs more detail. What cloud service are you using? How are you using it? Why are you trying to do an assembly? What results are you looking for?
Comments.UserDisplayName: gringer
Tags: <ngs><assembly><fastq><virus>
Title: DNASTAR viral-host integration assembly keeps failing
ThreadText: # DNASTAR viral-host integration assembly keeps failing
  
  (Asked by: username_0 on 2023-02-11)
  
  I have two NGS files from an NGS company corresponding to the sequencing data from a tumor sample as follows:
  
  * TB\_7710391\_R1.FASTQ.gz
  * TB\_7710391\_R2.FASTQ.gz
  
  I have downloaded the genome for MCPyV as a file called “MCPYV\_Sequence.fasta”
  
  I have downloaded the Hg38 as a file called “hg\_38.fa”
  
  I have input the host genome as hg\_38.fa, the viral genome as “ MCPYV\_Sequence.fasta” and the sequence data as both FASTQ files.
  
  I run the assembly in the cloud, and I receive back an error stating that the assembly as failed.
  
  What am I doing wrong?
  
  **Tags:** ngs, assembly, fastq, virus
  
  > **Comments:**
  > **username_1**: Please clarify your specific problem or provide additional details to highlight exactly what you need. As it's currently written, it's hard to tell exactly what you're asking.  
  > **username_2**: This question needs more detail. What cloud service are you using? How are you using it? Why are you trying to do an assembly? What results are you looking for?  
  
  ---
  
  ## 0 answers
  
  