Answers.Body: <p>I don't know of any online tool enabling a search against TrEMBL. You probably will have to set up your own local BLAST environment, roughly following these steps:</p>
  
  <ol>
  <li><a href="https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&amp;PAGE_TYPE=BlastDocs&amp;DOC_TYPE=Download" rel="nofollow noreferrer">download blast+</a> - but there might be an easier way to install the software, depending on your operating system (e.g. on Ubuntu you can just <code>sudo apt install ncbi-blast+</code>)</li>
  <li><a href="https://www.uniprot.org/downloads" rel="nofollow noreferrer">download target databases</a></li>
  <li><a href="https://www.ncbi.nlm.nih.gov/books/NBK279688/" rel="nofollow noreferrer">build local database</a></li>
  </ol>
  
  <p>I hope this helps.</p>
  
Answers.ContentLicense: CC BY-SA 4.0
Answers.CreationDate: 2020-04-25T20:58:54.317Z
Answers.Text: thank you a lot for your answer!unfortunately my programming skills are not so strong!
Answers.UserDisplayName: marilu
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Answers.UserDisplayName: mrhd
Answers.Body: <p>UniProt provides a BLAST service (<a href="https://www.uniprot.org/blast" rel="nofollow noreferrer">https://www.uniprot.org/blast</a>) which you can run against several UniProt databases, or you could use the Expasy BLAST too, at <a href="https://web.expasy.org/blast" rel="nofollow noreferrer">https://web.expasy.org/blast</a>.</p>
  
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Answers.UserDisplayName: Elisabeth.Gasteiger
Body: <p>i need to search for one protein against UNIPROT in BLAST for a selected organism.
  How can I do it? (SWSISSPROT+TREMBL)
  THANKS!!</p>
  
Tags: <blast><uniprot>
Title: how can i search in p-blast against UNIPROT(not only swissprot)
ThreadText: # how can i search in p-blast against UNIPROT(not only swissprot)
  
  (Asked by: username_0 on 2020-04-25)
  
  i need to search for one protein against UNIPROT in BLAST for a selected organism.
  How can I do it? (SWSISSPROT+TREMBL)
  THANKS!!
  
  **Tags:** blast, uniprot
  
  
  ---
  
  ## 2 answers
  
  ### Answer by username_1 on 2020-04-25. Score: 1 | Accepted answer
  
  I don't know of any online tool enabling a search against TrEMBL. You probably will have to set up your own local BLAST environment, roughly following these steps:
  
  1. [download blast+](https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastDocs&DOC_TYPE=Download) - but there might be an easier way to install the software, depending on your operating system (e.g. on Ubuntu you can just `sudo apt install ncbi-blast+`)
  2. [download target databases](https://www.uniprot.org/downloads)
  3. [build local database](https://www.ncbi.nlm.nih.gov/books/NBK279688/)
  
  I hope this helps.
  
  > **Comments:**
  > **username_0**: thank you a lot for your answer!unfortunately my programming skills are not so strong!  
  
  ---
  
  ### Answer by username_2 on 2020-10-23. Score: 0
  
  UniProt provides a BLAST service (<https://www.uniprot.org/blast>) which you can run against several UniProt databases, or you could use the Expasy BLAST too, at <https://web.expasy.org/blast>.
  
  
  ---
  
  