Answers.Body: <p>Honestly, ask who made them.  If the reads are from an organism without splicing, it will not be trivial to figure it out with 100% certainty.</p>
  <p>If you are calling variants, I don't think it matters much, except that higher organisms have such a small % of their genome transcribed, it should be obvious very quickly if you have the desired coverage across the entire genome, or just the transcripts.</p>
  
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Answers.CreationDate: 2021-03-10T20:33:53.050Z
Answers.LastActivityDate: 2021-03-10T20:33:53.050Z
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Body: <p>I have (gave me) Illumina fastq files, which I want to use for variant calling, and I do not know if they are DNAseq or RNAseq data. How can I check this? I do not have any report or who to ask.</p>
  <p>Many thanks</p>
  
Tags: <rna-seq><phylogenetics><fastq>
Title: Fastq: how can I check if they are from DNA or RNAseq data?
ThreadText: # Fastq: how can I check if they are from DNA or RNAseq data?
  
  (Asked by: username_0 on 2021-03-10)
  
  I have (gave me) Illumina fastq files, which I want to use for variant calling, and I do not know if they are DNAseq or RNAseq data. How can I check this? I do not have any report or who to ask.
  
  Many thanks
  
  **Tags:** rna-seq, phylogenetics, fastq
  
  
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  ## 1 answers
  
  ### Answer by username_1 on 2021-03-10. Score: 4
  
  Honestly, ask who made them. If the reads are from an organism without splicing, it will not be trivial to figure it out with 100% certainty.
  
  If you are calling variants, I don't think it matters much, except that higher organisms have such a small % of their genome transcribed, it should be obvious very quickly if you have the desired coverage across the entire genome, or just the transcripts.
  
  
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