Answers.Body: <p>A subject is an individual or donor. You can take multiple samples from the same subject, e.g. from different tissues, different timepoints etc.</p>
  
Answers.ContentLicense: CC BY-SA 4.0
Answers.CreationDate: 2021-07-31T12:24:28.950Z
Answers.LastActivityDate: 2021-07-31T12:24:28.950Z
Answers.UserDisplayName: Chris_Rands
Body: <p>I am reading <a href="https://www.nature.com/articles/s41592-019-0576-7" rel="nofollow noreferrer">this</a> paper, where the authors mention</p>
  <blockquote>
  <p>We apply SAUCIE to the batch correcting, denoising and clustering of
  an 11-million cell mass cytometry dataset with 180 samples from 40
  subjects in a study of the dengue flavivirus</p>
  </blockquote>
  <p>What exactly is the difference between a “sample” and a “subject” in scRNA-Seq?</p>
  <p>PS: here’s a possibly relevant visual from the paper, but I still couldn’t understand the difference
  <a href="https://i.sstatic.net/DEUUU.jpg" rel="nofollow noreferrer"><img src="https://i.sstatic.net/DEUUU.jpg" alt="enter image description here" /></a></p>
  
Tags: <scrnaseq>
Title: Sample versus subject in scRNA-seq
ThreadText: # Sample versus subject in scRNA-seq
  
  (Asked by: username_0 on 2021-07-31)
  
  I am reading [this](https://www.nature.com/articles/s41592-019-0576-7) paper, where the authors mention
  
  > We apply SAUCIE to the batch correcting, denoising and clustering of
  > an 11-million cell mass cytometry dataset with 180 samples from 40
  > subjects in a study of the dengue flavivirus
  
  What exactly is the difference between a “sample” and a “subject” in scRNA-Seq?
  
  PS: here’s a possibly relevant visual from the paper, but I still couldn’t understand the difference
  [![enter image description here](https://i.sstatic.net/DEUUU.jpg)](https://i.sstatic.net/DEUUU.jpg)
  
  **Tags:** scrnaseq
  
  
  ---
  
  ## 1 answers
  
  ### Answer by username_1 on 2021-07-31. Score: 1 | Accepted answer
  
  A subject is an individual or donor. You can take multiple samples from the same subject, e.g. from different tissues, different timepoints etc.
  
  
  ---
  
  