Answers.Body: <p>There are several programs for simulating/predicting DNA melting but you could try them for RNA.
  You can look here <a href="http://bioinformatics.org/meltsim/wiki/Main/HomePage" rel="nofollow noreferrer">http://bioinformatics.org/meltsim/wiki/Main/HomePage</a>.</p>
  
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Answers.CreationDate: 2020-03-03T22:47:57.333Z
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Answers.Body: <p>Both <a href="https://biopython.org/docs/1.75/api/Bio.SeqUtils.MeltingTemp.html" rel="nofollow noreferrer">Bio.SeqUtils.MeltingTemp</a> of Biopython and <a href="https://primer3.org/" rel="nofollow noreferrer">Primer3</a> offers different methods/algorithms to calculate the Tm value.</p>
  
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Answers.CreationDate: 2021-07-27T10:44:00.697Z
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Body: <p>Is there any tool available to predict the melting temperature for rna/lna oligos with its perfect complement ? </p>
  
  <p>I know this amazing website from qiagen/geneglobe that estimates the tm value. </p>
  
  <p>But is there any command line tool that does this? </p>
  
  <p>I can estimate the normal tm value with RNAcofold and the -T option but there is no lna option. </p>
  
  <p>I searched for a while but i can only find online services. </p>
  
Tags: <rna>
Title: Tool for rna/lna melting temperature prediction
ThreadText: # Tool for rna/lna melting temperature prediction
  
  (Asked by: username_0 on 2020-03-03)
  
  Is there any tool available to predict the melting temperature for rna/lna oligos with its perfect complement ?
  
  I know this amazing website from qiagen/geneglobe that estimates the tm value.
  
  But is there any command line tool that does this?
  
  I can estimate the normal tm value with RNAcofold and the -T option but there is no lna option.
  
  I searched for a while but i can only find online services.
  
  **Tags:** rna
  
  
  ---
  
  ## 2 answers
  
  ### Answer by username_1 on 2020-03-03. Score: 1
  
  There are several programs for simulating/predicting DNA melting but you could try them for RNA.
  You can look here <http://bioinformatics.org/meltsim/wiki/Main/HomePage>.
  
  
  ---
  
  ### Answer by username_2 on 2021-07-27. Score: 1
  
  Both [Bio.SeqUtils.MeltingTemp](https://biopython.org/docs/1.75/api/Bio.SeqUtils.MeltingTemp.html) of Biopython and [Primer3](https://primer3.org/) offers different methods/algorithms to calculate the Tm value.
  
  
  ---
  
  