Body: <p>Is there a method to download all the sequences for a particular geographic region (Panama) for 'DNA barcode' sequences (ITS2, rbcL or trnL). Hopefully, the specimen collection location will include this information.</p>
  <p>I want to use the Genbank sequences to create a DNA reference database of local plants for eDNA studies.</p>
  
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2020-06-03T06:22:38.683Z
Comments.Text: Hi @uvnomad yes its easy, but what does your reference database do? Is it for taxonomy of all plants?
Comments.UserDisplayName: M__
Comments.ContentLicense: CC BY-SA 4.0
Comments.CreationDate: 2020-06-03T19:33:05.153Z
Comments.Text: Great, how is it done? The reference is used to compare sequences of unknown plants (eDNA) to the known sequences of locally occuring plants.
Comments.UserDisplayName: uvnomad
Tags: <phylogenetics><barcode>
Title: mining DNA barcodes from Genebank/BOLD per location
ThreadText: # mining DNA barcodes from Genebank/BOLD per location
  
  (Asked by: username_0 on 2020-06-02)
  
  Is there a method to download all the sequences for a particular geographic region (Panama) for 'DNA barcode' sequences (ITS2, rbcL or trnL). Hopefully, the specimen collection location will include this information.
  
  I want to use the Genbank sequences to create a DNA reference database of local plants for eDNA studies.
  
  **Tags:** phylogenetics, barcode
  
  > **Comments:**
  > **username_1**: Hi @username_0 yes its easy, but what does your reference database do? Is it for taxonomy of all plants?  
  > **username_0**: Great, how is it done? The reference is used to compare sequences of unknown plants (eDNA) to the known sequences of locally occuring plants.  
  
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  ## 0 answers
  
  