SQ1002 is a 191nt small RNA identified in "Clostridioides difficile" 630 by RNA deep sequencing and confirmed by Northern blot and RT-PCR.
Extremities were confirmed by 5’ and 3’-RACE.
This RNA is found in several "C. difficile" strains at syntenic positions between a putative acyltransferase gene and a ferrous iron transport gene. No homologs were found outside "C. difficile", although a putative paralog was found in "C. difficile" 630, located between a tryptophanyl-tRNA synthetase gene and a pseudogene.
Due to lack of sequence variation, the proposed secondary structure is not supported by any covariation and should be considered speculative.
References.
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