Enzyme
Pullulanase (EC 3.2.1.41, "limit dextrinase", "amylopectin 6-glucanohydrolase", "bacterial debranching enzyme", "debranching enzyme", "α-dextrin endo-1,6-α-glucosidase", "R-enzyme", "pullulan α-1,6-glucanohydrolase") is a specific kind of glucanase, an amylolytic exoenzyme, that degrades pullulan. It is produced as an extracellular, cell surface-anchored lipoprotein by Gram-negative bacteria of the genus "Klebsiella". Type I pullulanases specifically attack α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages. It is also produced by some other bacteria and archaea. Pullulanase is used as a processing aid in grain processing biotechnology (production of ethanol and sweeteners).
Pullulanase is also known as pullulan-6-glucanohydrolase (Debranching enzyme). Its substrate, pullulan, is regarded as a chain of maltotriose units linked by α-1,6-glycosidic bonds. Pullulanase will hydrolytically cleave pullulan (α-glucan polysaccharides).
Pullulanase enzyme in the food industry.
In the food industry, pullulanase works well as an ingredient. Pullulan can be applied directly to foods as a protective glaze or edible film due to its ability to form films. It can be used as a spice and flavoring agent for micro-encapsulation. It is used in mayonnaise to maintain consistency and quality. It is additionally used in low-calorie food formulations as a starch replacement.
Pullulanase can be used to convert starches in grains into fermentable sugars, which yeast can use to produce alcohol during fermentation.
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