Class of enzymes
Azobenzene reductase also known as azoreductase (EC 1.7.1.6) is an enzyme that catalyzes the chemical reaction:
N,N-dimethyl-1,4-phenylenediamine + aniline + NADP+ formula_1 4-(dimethylamino)azobenzene + NADPH + H+
The 3 substrates of this enzyme are N,N-dimethyl-1,4-phenylenediamine, aniline, and nicotinamide adenine dinucleotide phosphate ion, whereas its 3 products are 4-(dimethylamino)azobenzene, nicotinamide adenine dinucleotide phosphate, and hydrogen ion.
This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with NAD+ or NADP+ as acceptor.
Mechanism.
The reaction catalyzed by this enzyme proceeds via a ping-pong mechanism by using 2 equivalents of NAD(P)H to reduce one equivalent of the azo compound substrate (for example methyl red where Ar = "p"-dimethylaniline and Ar' = "o"-benzoic acid) into two equivalents of aniline product:
 Ar–N=N–Ar' + 2(NAD(P)H + H+) formula_1 Ar–NH2 + NH2–Ar' + 2NAD(P)+
Substrate specificity.
Most azoreductase isoenzymes can reduce methyl red, but are not able to reduce sulfonated azo dyes. The unique azoreductase isozyme from "Bacillus" sp. B29 has the ability to reduce sulfonated azo dyes however.
Nomenclature.
The systematic name of this enzyme class is N,N-dimethyl-1,4-phenylenediamine, aniline:NADP+ oxidoreductase. Other names in common use include:
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Structural studies.
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1NNI, 1V4B, and 2D5I. Please check the last updated data on RCSB PDB site.
References.
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Further reading.
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