The PTS Glucitol (Gut) Family (TC# 4.A.4)consists only of glucitol-specific porters, but these occur both in Gram-negative and Gram-positive bacteria. It is part of the PTS-GFL superfamily.
Structure.
IIGut of "Escherichia coli" consists of three proteins, a IIA protein, a putative 4 TMS IIC2 protein and a putative 4 TMS IIC1 protein. The N- and C-termini as well as the IIB domain may thereby be localized to the cell cytoplasm, but the topology has not been established experimentally. IIAGut is believed to be phosphorylated on a histidyl residue, while IIBGut is probably phosphorylated on a cysteyl residue. However, these possibilities have not been demonstrated experimentally.
References.
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